CN103881819A - Process for enriching linolenic acid in linseed oil by beta-cyclodextrin clathration method - Google Patents
Process for enriching linolenic acid in linseed oil by beta-cyclodextrin clathration method Download PDFInfo
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- CN103881819A CN103881819A CN201210557157.5A CN201210557157A CN103881819A CN 103881819 A CN103881819 A CN 103881819A CN 201210557157 A CN201210557157 A CN 201210557157A CN 103881819 A CN103881819 A CN 103881819A
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- linolenic acid
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Abstract
The invention discloses a process for enriching linolenic acid in linseed oil by a beta-cyclodextrin clathration method, which comprises the following steps: preparing linseed oil, preparing aliphatic acid by mixing with linseed oil, separating alpha-linolenic acid by the beta-cyclodextrin clathration method, and extracting linolenic acid. The process for enriching the linolenic acid in linseed oil by the beta-cyclodextrin clathration method has the advantages of simple process, easy operation, low cost and high yield, and is suitable for large-scale industrial production.
Description
Technical field
The invention belongs to biological technical field, be specifically related to linolenic technique in beta-cyclodextrin inclusion compound method enrichment oleum lini.
Background technology
Flax is annual herbaceous plant, for one of the world today ten large oil crops, contains rich in protein and fat in linseed oil.Oleum lini is a kind of lipid acid mixture, wherein contains the multiple lipid acid such as palmitinic acid, stearic acid, linolic acid, oleic acid, linolenic acid, and wherein alpha-linolenic acid content accounts for the 40%-60% of total fatty acids.
Alpha-linolenic acid is essential fatty acid, still forms the basic substance of cytolemma and biological enzyme, simultaneously in the balance that maintains fat egg, fish tallow falls, the metabolism of cholesterol regulating, step-down, antithrombotic, the aspect tools such as prevention canceration play a very important role, and are a kind of good pure natural healthcare products.Beta-cyclodextrin (β-CD) be have D type Glucopyranose to join end to end by α Isosorbide-5-Nitrae-glycosidic link and circlewise sugar.There is hydrophobic truncated cone-shaped cavity and hydrophilic surface.By the interaction of intramolecular reactive force (as Van der Waals force, hydrophobic interaction power and hydrogen bond etc.), guest molecule polarity size, ionized state, the three-dimensional construction of hand-type etc., embody the recognition capability of cyclodextrine to object.Prior art separates linolenic acid complex process from oleum lini, and cost is high, and yield is low, is not suitable for suitability for industrialized production.
Summary of the invention
The above-mentioned defect existing in order to overcome prior art field, the object of the invention is to, and linolenic technique in a kind of beta-cyclodextrin inclusion compound method enrichment oleum lini is provided, solve prior art and from oleum lini, separate linolenic acid complex process, cost is high, and yield is low, is not suitable for the problem of suitability for industrialized production.
Linolenic technique in beta-cyclodextrin inclusion compound method enrichment oleum lini provided by the invention, comprises the following steps:
One, the preparation of linseed oil, linseed oil is after pulverizing, filter paper parcel, filter paper packet is placed in Soxhlet extraction flask and adds sherwood oil to Florence flask 2/3 place, and Soxhlet extraction device is placed in 45 ℃ of thermostat water baths, extract 3 hours, stop heating, after cooling, take out filter paper packet, by Florence flask and by reclaiming sherwood oil in inner rotary furnace pot, surplus full grease is linseed oil;
Two, the preparation of linseed oil mixed fatty acid, in adding 5%NaOH ethanolic soln to mix in the linseed oil of known quality to be placed on water-bath, saponification refluxes 1 hour, in saponified, add appropriate hot water dissolving, with a small amount of petroleum ether extraction 2 times, remove saponified, lower floor's solution is adjusted PH to 2-3 with 10%HCl, put shunting funnel stratification, get top oil reservoir, be washed till neutrality with distilled water, put into 60 ℃ of ancient customs loft drier dry 1 hour, remove moisture content and obtain oleum lini mixed fatty acid;
Three, beta-cyclodextrin inclusion compound method separating alpha-linolenic acid, take a certain amount of beta-cyclodextrin and add appropriate distilled water, saturated solution is made in heating, constant temperature is stirred to 50 ℃, adds linolenic acid mixed fatty acid and dehydrated alcohol, stirs constant temperature to 50 ℃ 2 hours, cooling rearmounted refrigerator freezing 20 hours, dissolve filtering and washing, put 80 ℃ of thermostat containers dry 3 hours, obtain white powder β-linolenic acid-Benexate Hydrochloride;
Four, linolenic acid extracts, and by white powder inclusion compound, puts in flask, after adding 40 ℃ of a certain amount of sherwood oils to reflux 1 hour, and decompress filter, sherwood oil is reclaimed in evaporation, and the non-volatile lipid acid in the bottle end be the abundant lipid acid containing alpha-linolenic acid.
Linolenic technique in beta-cyclodextrin inclusion compound method enrichment oleum lini provided by the invention, its beneficial effect is, and technique is simple to operation, and cost is lower, and yield is high, is applicable to large-scale industrialization and produces.
Embodiment
Below in conjunction with an embodiment, linolenic technique in beta-cyclodextrin inclusion compound method enrichment oleum lini provided by the invention is described in detail.
Embodiment
Linolenic technique in the beta-cyclodextrin inclusion compound method enrichment oleum lini of the present embodiment, comprises the following steps:
One, the preparation of linseed oil, linseed oil is after pulverizing, filter paper parcel, filter paper packet is placed in Soxhlet extraction flask and adds sherwood oil to Florence flask 2/3 place, and Soxhlet extraction device is placed in 45 ℃ of thermostat water baths, extract 3 hours, stop heating, after cooling, take out filter paper packet, by Florence flask and by reclaiming sherwood oil in inner rotary furnace pot, surplus full grease is linseed oil;
Two, the preparation of linseed oil mixed fatty acid, in adding 5%NaOH ethanolic soln to mix in the linseed oil of known quality to be placed on water-bath, saponification refluxes 1 hour, in saponified, add appropriate hot water dissolving, with a small amount of petroleum ether extraction 2 times, remove saponified, lower floor's solution is adjusted PH to 2-3 with 10%HCl, put shunting funnel stratification, get top oil reservoir, be washed till neutrality with distilled water, put into 60 ℃ of ancient customs loft drier dry 1 hour, remove moisture content and obtain oleum lini mixed fatty acid;
Three, beta-cyclodextrin inclusion compound method separating alpha-linolenic acid, take a certain amount of beta-cyclodextrin and add appropriate distilled water, saturated solution is made in heating, constant temperature is stirred to 50 ℃, adds linolenic acid mixed fatty acid and dehydrated alcohol, stirs constant temperature to 50 ℃ 2 hours, cooling rearmounted refrigerator freezing 20 hours, dissolve filtering and washing, put 80 ℃ of thermostat containers dry 3 hours, obtain white powder β-linolenic acid-Benexate Hydrochloride;
Four, linolenic acid extracts, and by white powder inclusion compound, puts in flask, after adding 40 ℃ of a certain amount of sherwood oils to reflux 1 hour, and decompress filter, sherwood oil is reclaimed in evaporation, and the non-volatile lipid acid in the bottle end be the abundant lipid acid containing alpha-linolenic acid.
Claims (1)
1. a linolenic technique in beta-cyclodextrin inclusion compound method enrichment oleum lini, is characterized in that: comprise the following steps:
One, the preparation of linseed oil, linseed oil is after pulverizing, filter paper parcel, filter paper packet is placed in Soxhlet extraction flask and adds sherwood oil to Florence flask 2/3 place, and Soxhlet extraction device is placed in 45 ℃ of thermostat water baths, extract 3 hours, stop heating, after cooling, take out filter paper packet, by Florence flask and by reclaiming sherwood oil in inner rotary furnace pot, surplus full grease is linseed oil;
Two, the preparation of linseed oil mixed fatty acid, in adding 5%NaOH ethanolic soln to mix in the linseed oil of known quality to be placed on water-bath, saponification refluxes 1 hour, in saponified, add appropriate hot water dissolving, with a small amount of petroleum ether extraction 2 times, remove saponified, lower floor's solution is adjusted PH to 2-3 with 10%HCl, put shunting funnel stratification, get top oil reservoir, be washed till neutrality with distilled water, put into 60 ℃ of ancient customs loft drier dry 1 hour, remove moisture content and obtain oleum lini mixed fatty acid;
Three, beta-cyclodextrin inclusion compound method separating alpha-linolenic acid, take a certain amount of beta-cyclodextrin and add appropriate distilled water, saturated solution is made in heating, constant temperature is stirred to 50 ℃, adds linolenic acid mixed fatty acid and dehydrated alcohol, stirs constant temperature to 50 ℃ 2 hours, cooling rearmounted refrigerator freezing 20 hours, dissolve filtering and washing, put 80 ℃ of thermostat containers dry 3 hours, obtain white powder β-linolenic acid-Benexate Hydrochloride;
Four, linolenic acid extracts, and by white powder inclusion compound, puts in flask, after adding 40 ℃ of a certain amount of sherwood oils to reflux 1 hour, and decompress filter, sherwood oil is reclaimed in evaporation, and the non-volatile lipid acid in the bottle end be the abundant lipid acid containing alpha-linolenic acid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210557157.5A CN103881819A (en) | 2012-12-20 | 2012-12-20 | Process for enriching linolenic acid in linseed oil by beta-cyclodextrin clathration method |
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| CN201210557157.5A CN103881819A (en) | 2012-12-20 | 2012-12-20 | Process for enriching linolenic acid in linseed oil by beta-cyclodextrin clathration method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106942359A (en) * | 2017-03-15 | 2017-07-14 | 合肥华创现代农业科技有限公司 | A kind of edible fruit and vegetable fresh-keeping agent and preparation method thereof |
| CN107556187A (en) * | 2017-08-31 | 2018-01-09 | 江苏科技大学 | Beta cyclodextrin embedding combined simulation mobile bed chromatic partition method prepares the linolenic method of high-purity alpha |
| CN109090702A (en) * | 2018-06-29 | 2018-12-28 | 郑州轻工业学院 | The preparation method and applications of Luo Han Guo Paste In Foods maillard reaction product |
| CN113331366A (en) * | 2021-03-27 | 2021-09-03 | 西藏双湖县普若岗日生物科技有限公司 | High-activity artemia cyst oil for improving sleep disorder and preparation method thereof |
-
2012
- 2012-12-20 CN CN201210557157.5A patent/CN103881819A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106942359A (en) * | 2017-03-15 | 2017-07-14 | 合肥华创现代农业科技有限公司 | A kind of edible fruit and vegetable fresh-keeping agent and preparation method thereof |
| CN107556187A (en) * | 2017-08-31 | 2018-01-09 | 江苏科技大学 | Beta cyclodextrin embedding combined simulation mobile bed chromatic partition method prepares the linolenic method of high-purity alpha |
| CN109090702A (en) * | 2018-06-29 | 2018-12-28 | 郑州轻工业学院 | The preparation method and applications of Luo Han Guo Paste In Foods maillard reaction product |
| CN109090702B (en) * | 2018-06-29 | 2021-08-24 | 郑州轻工业学院 | Preparation method and application of Maillard reaction product of fructus momordicae extract |
| CN113331366A (en) * | 2021-03-27 | 2021-09-03 | 西藏双湖县普若岗日生物科技有限公司 | High-activity artemia cyst oil for improving sleep disorder and preparation method thereof |
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Application publication date: 20140625 |