CN103877612B - Cytoskeleton of a kind of carbon nanotubes and preparation method thereof - Google Patents

Abstract

The invention discloses cytoskeleton of a kind of carbon nanotubes and preparation method thereof.By being mixed according to a certain ratio with CNT by PLGA, improve the agglomeration of CNT.By electrostatic spinning technique, the PLGA solution that with the addition of CNT is made micro-nano three-dimensional cell support.Add the PLGA support of CNT, its mechanical property and the purer PLGA support of hydrophilic have had and have significantly improved.Cell experiment shows, cell has good adhesiveness and grows vigorous on the material of carbon nanotubes, and the cell on simple PLGA support then more easily comes off, and all can not keep its normal form.Therefore the PLGA cytoskeleton of carbon nanotubes is with a wide range of applications in field of tissue engineering technology particularly nervous tissue, heart tissue, bone tissue engineering scaffold.

Description

Cytoskeleton of a kind of carbon nanotubes and preparation method thereof

Technical field

The invention belongs to tissue engineering technique field, relate to cytoskeleton of a kind of carbon nanotubes and preparation method thereof, Poly(D,L-lactide-co-glycolide three-dimensional cell support being specially carbon nanotubes and preparation method thereof.

Background technology

Organizational project can realize the regeneration of tissue.Tissue engineering bracket can provide the basis needed for growth for the cell be damaged because of reasons such as disease, damage, birth defects.This timbering material must possess the performances such as biocompatibility, biodegradability, high-voidage, mechanics adaptability.Electrostatic spinning technique can prepare the identical three-dimensional porous fiber with natural extracellular matrix structure, and this fibrous membrane as desirable tissue engineering bracket, can be convenient to the adhesion of target cell, propagation and migration.

Along with the research of electrostatic spinning technique is goed deep into gradually, multiple synthesis macromolecule and natural polymer raw material is had to prepare nanofiber for electrostatic spinning technique at present ^[1].Poly(D,L-lactide-co-glycolide (PLGA) is a kind of degradable functional polymer organic compound, has good biological degradability, cell compatibility and processability, is widely used in tissue engineering material ^[2].High polymer PLGA is ratified by U.S. FDA, can be applicable to the biomaterial that alternative human body is organized originally.The PLGA fibrous framework utilizing electrostatic spinning technique to prepare, is widely used in load biological source cell or human archeocyte to rebuild engineered nerve ^[3], engineered skeleton ^[4], tissue engineering skin ^[5]etc. the research of engineered tissue.But current simple PLGA timbering material existing defects, such as first often there is the single uncontrollable shortcoming of mechanical property in its engineered organizing built, and tissue-engineered bone can not be widely used in, tissue engineering skin, the hardness of the tissue substitute that engineered muscle etc. are different and intensity need, secondly cannot meet when building tissue engineered peripheral nerve and organizing, need the electrical performance requirements such as the bioelectric current conduction between nerve synapse link, limit the range of application of simple PLGA biologic bracket material.

CNT (CNTs) has unique structure, strengthens, has outstanding performance in semi-conducting material catalyst carrier etc. in the nanometer of engineering material.In addition, CNTs has the photoelectric property of high stretch modulus, good heat conduction and electric conductivity and uniqueness, makes it have wide practical use improving to gather around in the mechanics, electricity, calorifics, optics etc. of polymeric material.Along with the maturation of technology of preparing and the continuous reduction of cost, the applied research of CNTs more and more comes into one's own.

Taking into account both advantages simultaneously, thus show that the material being more applicable to organizational project use is very important, utilizing electrostatic spinning blending technology just can be provided important approach by the mechanics of the PLGA material of FDA certification and biology performance for improving.But when carrying out different materials mixed processed, the impact of positive and negative both direction can be produced on original material system, the feature of the high-specific surface area that CNTS itself has should be retained, overcome its nanometer toxicity again, how selective response raw material and reaction system, on the basis determining reaction raw materials and system, how adopting full-bodied PLGA to wrap up it and modify and effective could reduce this nanometer toxicity to the impact etc. of formative tissue, is all those skilled in the art's technical barriers urgently to be resolved hurrily.

Summary of the invention

In order to improve the deficiency of simple PLGA biologic bracket material, CNTs and PLGA bi-material combines by the present invention, is applicable to the cytoskeleton of engineered tissue and nervous tissue by electrostatic spinning technique manufacture.First, invention describes a kind of method preparing even CNTs suspension simply and easily, for the electrostatic spinning of subsequent bio support provides guarantee.Secondly, the PLGA biological support containing CNTs prepared by the present invention, has excellent mechanical property, electric conductivity performance and biological property concurrently.Along with the changes of contents of CNT in composite material, the different performance of material monolithic can be made to produce corresponding change, this main manifestations is for when CNTs content is higher, the impact for material monolithic electric conductivity occurred, and when CNTs content is lower, the impact for material monolithic mechanical property of generation.Meanwhile, the high-specific surface area ratio that CNTs itself has, also makes it be used alone the controversial nanometer toxicity of tool, adopts full-bodied PLGA to wrap up it and modifies and effectively can reduce this nanometer toxicity to the impact of formative tissue.The present invention is directed to the impact of CNT on material mechanical performance, obtain a kind of preparation component and method of biological support, this biological support is expected to the construction work being effectively applied to the engineered tissues such as skeleton, skin and nerve.

The present invention is with Poly(D,L-lactide-co-glycolide (PLGA), hexafluoroisopropanol (HFIP), CNT (CNTS) is raw material, first CNTs powder is made uniform suspension, after mixing with the PLGA parent spinning liquid under same solution system again, drop into electrostatic spinning.

A cytoskeleton preparation method for carbon nanotubes, it comprises following operating procedure:

A. PLGA is dissolved in obtained PLGA solution in HFIP;

B. CNTs is added obtained CNTs suspension in HFIP; The proportion of the PLGA:CNTs:HFIP in described step a and b is 0.7g ~ 2.1g:0.007 ~ 0.21g:7ml; The scope of further optimization is 0.7g ~ 1.4g:0.07 ~ 0.14g:7ml.

C., after PLGA solution and the mixing of CNTs suspension being obtained electrostatic spinning liquid, electrostatic spinning is carried out.

In technique scheme, the proportion of the PLGA:HFIP in described step a is 0.7g ~ 2.1g:2 ~ 5ml; The proportion of the CNTs:HFIP in described step b is 0.007 ~ 0.21g:2 ~ 5ml;

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, the ratio of the PLGA:CNTs:HFIP in step a described in it and b is 1.05g:0.014g:7ml.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, the CNTs suspension in step b described in it is through supersound process at least 1h.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, the PLGA solution of step a described in it is prepared through heating in water bath 4 ~ 5h.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, PLGA solution and the CNTs suspension hybrid mode of step c described in it are under room temperature, magnetic stirring apparatus stir at least 1h.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, the electrostatic spinning of step c described in it is be 15kv at electrostatic high-pressure, and the distance between dash receiver and shower nozzle is 10cm, and electrostatic spinning flow velocity is carry out under the condition of 1ml/h.Select No. 9 flattened metal pins.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, is characterized in that, described CNTs is CNT, unimolecule length 10 ~ 20 μm, and this kind of cytoskeleton preparation method is applicable to all carbon nano-tube materials.Unimolecule length 10-20 μm is embodiment employing, is equally suitable for this kind of method for other CNTS materials.

In technique scheme, the cytoskeleton preparation method of described carbon nanotubes, is characterized in that, described PLGA is LA/GA=85/15, M=1 × 10 ⁵, and this kind of cytoskeleton preparation method is applicable to existing all PLGA macromolecular materials.

Another aspect of the present invention is the cytoskeleton protecting a kind of carbon nanotubes utilizing said method to prepare.

Beneficial effect:

1 cytoskeleton utilizing the method for the invention to prepare, combines the advantage of PLGA and CNTs bi-material, improves the deficiency of simple PLGA biologic bracket material, has the advantages such as excellent mechanical property and biological property concurrently.In biomechanics, hydrophilic, porosity, cell adhesion and growth etc., all there is good effect, in embodiment, select mechanical stretch experiment, hydrophilicity experiment, scanning electron microscope and cell experiment data analysis all to demonstrate the effect utilizing described method to prepare biological support.

2 CNTs preprocess methods of the present invention, difficult dissolved material is successfully applied in electrostatic spinning technique, describe a kind of preparation method of CNTs biological support, this biological support is expected to the construction work being effectively applied to the engineered tissues such as skeleton, skin and nerve simultaneously.

Accompanying drawing explanation

Accompanying drawing 3 width of the present invention:

Fig. 1 is to be measured group of sample P LGA15%(W/V)+CTNs0.2%(W/V) and the simple composition PLGA15%(W/V of control sample) scanning electron microscope (Fig. 1-a and 1-b) of bi-material, diameter Distribution (Fig. 1-c and 1-d), porosity (Fig. 1-f, wherein P refers to the simple composition timbering material of PLGA15%, C refers to PLGA15%+CNTS0.2% blending constituent material) and hydrophilic (Fig. 1-e is after wherein 5s refers to water drop contact material 5 second, measure contact angle, after 30s refers to water drop contact material 30s, measure contact angle) laboratory test results figure.Material wherein containing CNTs0.2% is more former not containing the pure PLGA material of CNTs, and aggregate performance is: PLGA15%+CNTs0.2% fibre diameter average is 1734.02 native 201.423nm, PLGA15% fibre diameter averages is 849.43 native 421.648nm.Increase CNTs composition in the fiber of simple PLGA15% after, material monolithic fibre diameter is caused to increase.The mono-composition fiber diameter of former PLGA15% mainly concentrates in 600-800nm interval, and after adding CNTs0.2% composition, allow fibre diameter mainly distribute and concentrate on 1600-1800nm, diameter Distribution concentrated area improves 1 times; Hydrophilic CNTs adds, and increases during 5s containing CNTs composite material hydrophilic more simple composition PLGA15% material hydrophilic, increases CNTs and the contact angle of material and water can be allowed to be reduced to about 80 ° (Fig. 1-e is left) from 120 °; Porosity, pure PLGA porosity is 57%, PLGA15%+CNTS0.2% material porosity is 81%, increases CNTS and can improve material porosity (Fig. 1-f).

Fig. 2 is to be measured group of sample P LGA15%(W/V)+CTNs0.2%(W/V) and the simple composition PLGA15%(W/V of control sample) the Experiments of Machanics testing result of bi-material, Fig. 2-A:PLGA and Fig. 2-B:PLGA+CNTs is the stress-strain diagram that bi-material shows in tensile failure experiment, and Fig. 2-C is the physical index being tested this material drawn by mechanical stretch of bi-material.As can be seen from result, increase the PLGA15%(W/V after CNTs composition 0.2% in the material)+CTNs0.2%(W/V) the overall mechanical property raising of material property, aggregate performance is: containing CNTs material in Young's modulus, yield strength, all apparently higher than pure PLGA material in breaking strain 3 physical indexs, former PLGA15% young modulus of material is 135.7MPa, PLGA15%(W/V)+CTNs0.2%(W/V) material, Young's modulus is 191.88MPa, and the strength of materials increases (Fig. 2-C, left); Yield strength is increased to 5.27Pa from 2.07Pa, and material becomes tough (Fig. 2-C, in); And breaking strain is increased to 212.6% by 120% of simple PLGA15% material, show mechanical strength and the toughness (Fig. 2-C, right) that overallly can improve fibrous material containing CNTs.

Fig. 3 is to be measured group of sample P LGA15%(W/V)+CTNs0.2%(W/V) and the simple composition PLGA15%(W/V of control sample) bioassay results of bi-material compound PC12 cell, Fig. 3 A-B is the burnt photo of copolymerization after living cells dyeing, Fig. 3 C-D is after cell compound, fixing also stereoscan photograph.Meanwhile, Fig. 3 A-C is for containing PLGA15%(W/V)+CTNs0.2%(W/V) content material, Fig. 3 B-D is pure PLGA material.By observing photo, can find to grow the PC12 cell containing CNTs composite material surface, show the characteristic of higher division quantity and more affinitive material, cell can stretch out normal projection sticking and fiber (Fig. 3-A white arrow) containing CNTs material surface at edge, fiber also can be allowed simultaneously to pass cell space gap and to participate in cell own growth and fixing (Fig. 3-C white arrow) directly, containing CNT electrospinning material really with active somatic cell compound in show advantage.Demonstrating increases CNTs and has very large effect for the biocompatibility of material monolithic.

Detailed description of the invention

Following nonlimiting examples can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.

The present invention is raw materials used to be mainly contained:

Solute: Poly(D,L-lactide-co-glycolide; CNT (CNTs): unimolecule length 10 ~ 20 μm; It is the nano material comprising many walls and single tube material.

Dicyandiamide solution: hexafluoroisopropanol (HFIP) analytical pure.

Embodiment 1

One. prepare to be measured group of sample: PLGA15%(W/V)+CTNs0.2%(W/V)

The preparatory stage of electrostatic spinning liquid, that is: the dicyandiamide solution dissolving the wider HFIP of spectrum and jointly exist as the different high molecular polymer of carrying is adopted, respectively PLGA and CNTs is prepared into hexafluoro b propanol macromolecular solution and suspension, remerge both, magnetic agitation mixing obtains the process of the spinning liquid of uniform composition.

1. be first dissolved in 3mlHFIP(half volume total solvent amount using taking 1.05g as the PLGA solid particle of electrostatic spinning main body high molecular polymer main body) in, 4 ~ 5h is to dissolving completely in water-bath (37 DEG C) heating, obtained PLGA solution, i.e. PLGA macromolecule spinning liquid matrix.

2. again the CNTs of 0.014g is added 4mlHFIP(half volume total solvent amount) in supersound process 2 hours, form the CNTs suspension that dispersion is homogeneous.After magnetic stirrer 1h, liquid appearance is rendered as carbon nanotube powder and is dispersed in suspension in hexafluoroisopropanol solution, and after ultrasonic 1h, liquid appearance is rendered as homogeneous black suspension.

3. finally two-component liquid (PLGA solution and CNTs suspension) that is good for pretreatment in previous step 1 and 2, that carried by HFIP is mixed obtained PLGA15%(W/V)+CTNs0.2%(W/V), be put on magnetic stirring apparatus, room temperature lower magnetic force forms the homogeneous PLGA spinning liquid containing CNTs0.2wt% after stirring 1h, outward appearance is rendered as homogeneous black thick liquid.Along with blend step carries out, CNTs is progressively dispersed in overall spinning precursor liquid.Stir and within 4 hours, make fully to be uniformly dispersed between composition, obtain electrostatic spinning liquid.

4. in the electrostatic spinning stage: electrostatic spinning liquid step 3 handled well adds in 10ml syringe, front end is connected with electrostatic spinning machine by No. 9 flattened metal syringe needles, is added on the incident pump of constant current and controls liquid inventory.The electrostatic high-pressure be carried between shower nozzle and receiving pole regulating high-pressure electrostatic output instrument to control in tele-release process is 15kv.The distance determination receiving range changed between dash receiver and shower nozzle is 10cm, and adjustment solution flow rate is 1ml/h.Spin 2 hours, after spinning terminates, the material be attached on reception aluminium foil is placed in fume hood together with aluminium foil and takes off after room temperature air dried overnight, be placed in large culture dish and preserve.The cytoskeleton of obtained carbon nanotubes of the present invention, i.e. to be measured group of sample: PLGA15%(W/V)+CTNs0.2%(W/V).

Two. preparation control sample: simple composition PLGA15%(W/V)

1. be dissolved in 7mlHFIP(half volume total solvent amount using taking 1.05g as the PLGA solid particle of electrostatic spinning main body high molecular polymer main body) in, 4 ~ 5h is to dissolving completely in water-bath (37 DEG C) heating, obtained simple composition PLGA15%(W/V) electrostatic spinning liquid.

2. prepare the simple composition PLGA15%(W/V of control sample material according to step 4 electrospinning process of embodiment 1).

Embodiment 2 surface topography and diameter Distribution

By to be measured group and control sample material: PLGA15%(W/V)+CTNs0.2%(W/V) and simple composition PLGA15%(W/V) cut out the square sample of 1cm × 1cm respectively, metal spraying half an hour, detect microscopic appearance under scanning electron microscope.Metal spraying and irradiating after scanning electron microscope, often kind of material selection 3 1000 × magnification fields are taken pictures, and accomplish that the visual field as far as possible can the fiber micro Distribution of representational embodiment selected materials.Often open stereoscan photograph and select 6 visuals field at random, 5 fiber measurement diameters are selected in each visual field at random, and often kind of concentration C NTs material selection 90 fibers, utilize imageJ software to carry out diameter measurement analysis to these fibers altogether.

Result and analysis: above-mentioned experimental data proves adding of CNTs, and the diameter of simple PLGA material fiber is increased.PLGA15%+CNTs0.2% fibre diameter average is: 1734.02 native 201.423nm, PLGA15% fibre diameter averages are: 849.43 native 421.648nm.Increase CNTs composition in the fiber of simple PLGA15% after, material monolithic fibre diameter is caused to increase.The mono-composition fiber diameter of former PLGA15% mainly concentrates in 600-800nm interval, and after adding CNTs0.2% composition, allow fibre diameter mainly distribute and concentrate on 1600-1800nm, diameter Distribution concentrated area improves 1 times.This also demonstrates in from macroscopic view to microscopic ranges, CNTs participates in in overall fibrous framework, the change of this material character, at it as biologic bracket material application is, be conducive to the porosity improving all materials, and provide the growing space be more suitable for after subsequent cell growth.

Embodiment 3 mechanics properties testing

The Young's modulus of material is obtained, yield strength, breaking strain by the tensile failure experiment of INSTRON5948 mechanical stretch detector.All material sample all have passed through following pretreatment before carrying out tension test: by be measured group and control sample material: PLGA15%(W/V)+CTNs0.2%(W/V) and simple composition PLGA15%(W/V) respectively cutting become the dumbbell structure of 5cm × 1cm, every part of material thickness spiral micrometer is measured.Two ends respectively reserve 1cm × 1cm square clip position, and work area is 3cm × 1cm, and often kind of material does 5 groups of parallel laboratory tests.

Tensile failure is tested: be clipped on hydraulic clip by material strips two ends, after fixing with 10mm/min speed constant speed shaft to two ends tractive material to Materials Fracture.Draw the stress-strain curves of often kind of material, drawn the Young's modulus (slope of curve during elastic properties of materials deformation) of this material by calculated curve, yield strength (i.e. maximum load/original area), and breaking strain (material maximum strain).

Result and analysis: above-mentioned experimental data proves adding of CNTs, significantly improves the mechanical property of simple PLGA material.Former PLGA15% young modulus of material is 135.7MPa, and after adding CNTs0.2% composition, material monolithic Young's modulus is increased to 191.88MPa, and the strength of materials is increased.Yield strength is increased to 5.27Pa from 2.07Pa, and after adding CNTs, material becomes tough.And breaking strain is increased to 212.6% by 120% of simple PLGA15% material, in sum, adding of CNTs0.2% makes the mechanical property of overall Electrospun Nanofibrous Materials: An obtain very large enhancing, and 3 mechanical index all increase.The mechanical strength that CNTs adds the material made increases, and electrospinning material can be made to enter the tissue engineering field of more high strength on the one hand, on the other hand in the just regulation and control of the strength of materials, also plays an important role.

Embodiment 4 hydrophilic detects

To be measured group and control sample material: PLGA15%(W/V)+CTNs0.2%(W/V) and simple composition PLGA15%(W/V) often organize random selecting three respectively, be 0.2mlPBS aqueous solution in material surface volume injected, each sample 5 contact angles.Single-lens reflex camera is utilized to irradiate the 5s of water droplet in the 30s of material surface, the photo of 30s two timing nodes.Calculate contact angle size.

Result and analysis: above-mentioned experimental data proves adding of CNTs, during water drop contact material (5s), increase containing CNTs composite material hydrophilic more simple composition PLGA15% material hydrophilic, increase CNTs and the contact angle of material and water can be allowed to be reduced to about 80 ° from 120 °.Can the strong hydrophobic surface (being obviously the microenvironment being unfavorable for that most of active somatic cell sticks and survives) that simple PLGA material list reveals be corrected to some extent and be improved.

Embodiment 5 porosity detects

To be measured group and control sample material: PLGA15%(W/V)+CTNs0.2%(W/V) and simple composition PLGA15%(W/V) respectively after metal spraying and scanning electron microscope, often kind of material selection 3 1000 × magnification fields are taken pictures, and accomplish that the visual field as far as possible can the fiber micro Distribution of representational embodiment selected materials.According to the method proposed in document, measure respectively often open Vtotal(in photo be 3D depth reconstruct after X, Y, Z axis coordinate product), Vsolid(scanning of materials Electronic Speculum 2D integrated value), according to formula porosity θ=1-(Vsolid/Vtotal) often opened the porosity value of material shown in SEM, obtain porosity meansigma methods and error.

Result and analysis: because CNTs adds, and load in each root fiber of microcosmic, electrospinning material fiber diameter Distribution after compound is increased, the porosity of integral material is so just made to increase, experimental result also provides checking, make material porosity increase after adding CNTs, to be exactly material be like this cell adhesion and further growth provide space and ensure.

Embodiment 6 biology performance detects

To be measured group and control sample material: PLGA15%(W/V)+CTNs0.2%(W/V) and simple composition PLGA15%(W/V) before biological experiment, need each 1h of positive and negative ultraviolet sterilization, with aseptic line tighten in the frozen mouth of pipe removing bottom surface.Salt balance soaked overnight in aseptic PBS, soaked overnight in basic NB culture medium, can be used for the outer dimensional culture of the neuron implantation body that extracts and test.

The cell of PC12 and material composite that stick 3 days are taken out from culture medium, 1 group is carried out after live cell fluorescent dyeing (calcein--hoechst33342) in copolymerization pyrocellulose Microscopic observation cell growth state, another organizes after 4% formaldehyde room temperature fixes half an hour, and metal spraying irradiates scanning electron microscope.

Result and analysis: above-mentioned experimental result shows, containing CNT electrospinning material PLGA15%(W/V)+CTNs0.2%(W/V) really with active somatic cell compound in show advantage, show as growth cell adhesion rate thereon to increase, biological behaviour is normal, and has the trend being grown to engineered tissue.PC12 cell is compounded in the material PLGA15%(W/V containing CNTs)+CTNs0.2%(W/V) on, there is good adhesion, cell can be attached on material preferably, substantially material surface can be paved with, stereoscan photograph can showed cell form, and live cell fluorescent result can show the activity of PC12 cell on material and animation.And growth all can not keep the fusiformis form (Fig. 3-A white arrow) of its normal condition at the PC12 cell of the mono-composition fiber material of PLGA, do not appear at containing CNTs material PLGA15%(W/V)+CTNs0.2%(W/V) surface PC12 cell wrap up fiber (Fig. 3-C white arrow) like that, and allow fiber to pass extracellular matrix, but be more gathered in material surface.This phenomenon is given prominence to and is indicated the feature that CNTs improves the fiber biological compatibility.

List of references

[1]LIboDeng,RobertJ,Young,IanA.SupercapacitancefromCelluloseandCarbonNanotubeNanocompositeFibers,ACSAppliedmeterialsandinterfaces,2013/59983-9990.

[2]SharonJ,Sequeira,DavidA,Soscia,TheregulationoffocaladhesioncomplexformationandsalivaryglandepithelialcellorganizationbynanofibrousPLGAscaffolds,Biomaterials4/2012,3175-3186

[3]Fatemehmottaghitalab,MehdiFarokhi,ArashZaminy,ABiosyntheticNerveGuideConduitBasedonSilk/SWNT/FibronectinNanocompositeforPeripheralNerveRegeneration.PLUSone30/9/2013.

[4]AnandkumarNandakuar,AnaBarradas,Jandeboe,LorenzoMoroni,Combiningtechnologiestocreatebioactivehybridscaffoldsforbonetissueengineering,Biomatter3:2,7/2013

[5]SangameshG.Kumbar,SyamPrasadNukavarapu,RoshanJames,ElectrospunPoly(lacticacid-co-glycolicacid)ScaffoldsforSkinTissueEngineering,Biomaterials,10/2008,4100-4107。

Claims (5)

1. a cytoskeleton preparation method for carbon nanotubes, is characterized in that, comprises following operating procedure:

A. PLGA is dissolved in obtained PLGA solution in HFIP;

B. CNTs is added obtained CNTs suspension in HFIP; The proportion of the PLGA:CNTs:HFIP in described step a and b is 0.7g ~ 2.1g:0.007 ~ 0.21g:7ml;

C., after PLGA solution and the mixing of CNTs suspension being obtained electrostatic spinning liquid, electrostatic spinning is carried out;

Wherein: the PLGA solution of described step a is prepared through heating in water bath 4 ~ 5h; CNTs suspension in described step b is through supersound process at least 1h; PLGA solution and the CNTs suspension hybrid mode of described step c are under room temperature, magnetic stirring apparatus stir at least 1h; The electrostatic spinning of described step c is be 15kv at electrostatic high-pressure, and the distance between dash receiver and shower nozzle is 10cm, and electrostatic spinning flow velocity is carry out under the condition of 1ml/h.

2. the cytoskeleton preparation method of carbon nanotubes according to claim 1, it is characterized in that, the ratio of the PLGA:CNTs:HFIP in described step a and b is 1.05g:0.014g:7ml.

3. the cytoskeleton preparation method of carbon nanotubes according to claim 1, it is characterized in that, described CNTs is CNT, unimolecule length 10 ~ 20 μm.

4. the cytoskeleton preparation method of carbon nanotubes according to claim 1, it is characterized in that, described PLGA is LA/GA=85/15, M=1 × 10 ⁵.

5. the cytoskeleton of the carbon nanotubes utilizing method described in claim 1 to prepare.