CN103877583A - Cellulosa membrane structure-like gene carrier and preparation method thereof - Google Patents
Cellulosa membrane structure-like gene carrier and preparation method thereof Download PDFInfo
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- CN103877583A CN103877583A CN201410136235.3A CN201410136235A CN103877583A CN 103877583 A CN103877583 A CN 103877583A CN 201410136235 A CN201410136235 A CN 201410136235A CN 103877583 A CN103877583 A CN 103877583A
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Abstract
The invention discloses a cellulosa membrane structure-like gene carrier which is prepared from monomers and polycation by using a radical polymerization method, wherein the monomers comprise a vinyl monomer and an aniline monomer containing zwitter-ion hydrophilia groups; the polycation is chitosan, polyethyleneimine, polylysine or arborization polyurethane. In addition, the invention further discloses a preparation method of the cellulosa membrane structure-like gene carrier. The particle size of the cellulosa membrane structure-like gene carrier is small, namely, about 300-600nm, the surface Zeta potential is high, and the carrying capability of the carrier to DNA (Deoxyribonucleic Acid) is remarkably improved. The protein adsorption and the blood platelet adhesion of the cellulosa membrane structure-like gene carrier are greatly reduced, the biocompatibility is greatly improved, the in-vivo circulation time of the gene carrier is prolonged, and the medicine effect is improved.
Description
Technical field
The invention belongs to nanotechnology and biological medical polymer material technical field, be specifically related to a kind of imitating cell outer-layer membrane structure genophore and preparation method thereof.
Background technology
Gene therapy is to adopt defective gene in the gene substitution of normal function or supplement patient body, thereby reaches the object for the treatment of disease, has become one and has treated the range gene disease effective way of (comprising infectious disease, cancer etc.).In numerous genophores, particularly hypotoxic polycation gene carrier (Langmuir in non-viral gene vector, 2009,25 (9): 5199-5208) enjoy various countries scholar's attention, there is important learning value and huge application prospect.
Chitosan has the advantages (Carbohydrate Polymers, 2010,79 (3): 724-730) such as degradability, antibiotic property, nontoxic, non-stimulated, pH response, has been widely used in the fields such as biomedical.Chitosan is natural polycation, can, with polyanion by electrostatic interaction, the two strand is wound around mutually, finally forms nanoscale aggregation (Carbohydrate Polymers, 2005,62 (2): 142-158).In field of gene, chitosan, as polycation, by electrostatic interaction, in carrying medicament, forms the nano-particle of surface band positive charge with electronegative DNA.This nano-particle is by slowing down DNA degradation, inactivation, and adherent cell film improves endocytosis, relies on proton sponge effect to flee from endosome, thereby reaches the object (Biomacromolecules, 2009,10 (9): 2436-2445) that improves transfection efficiency.Therefore, this class chitosan composite polyelectrolyte nano-particle has become one of emphasis that nanometer medicine-carried system studies aspect gene therapy.
But, the compound polyelectrolyte surface that polycation and DNA form is with the positive charge of part, easy adhesion protein in circulation in vivo, then cause platelet, cell adhesion, cause forming thrombosis and immunoreation (Biomaterials, 2009,30 (34): 6655-6664), easily by monokaryon macrophage phagocytic, thereby reduce drug effect.Therefore the biocompatibility nano material of design stability is the key subject of pharmaceutical carrier design.
Phosphorylcholine (phosphorylcholine, PC) be the terminal hydrophyllic group of composition cell membrane elementary cell lecithin, it is the outer functional group in the tunic of extracellular, simultaneously with positive and negative xenogenesis electric charge, there is ability and the hydrophilicity of stronger combination water, the surface of this structure and composition and physiological environment interact and not only can not adsorb and depositing proteins, also can not cause platelet activation, cause the untoward reaction such as blood coagulation, have good biocompatibility.Research in recent years shows, adopts Phosphorylcholine to have imitating cell outer-layer membrane structure at chitosan surface construction, can significantly improve the biocompatibility of material.But these modification mode are all approach (Carbohydrate Polymers, 2007,70 (1): 82-88 that adopt the grafting of Phosphorylcholine micromolecule; ZL200410054022.2; Biomacromolecules, 2007,8 (10), 3169-3176; Biomacromolecules, 2006,7 (11): 3151-3156; Journal of Applied Polymer Science, 2003,88 (2): 489-493; Polymer International, 2003,52 (1): 81-85; Journal of biomaterials science, Polymer edition, 2002,13 (5): 501-510; Colloids and Surfaces B:Biointerfaces, 2009,71 (2): 268-274), often not high in the density of the Phosphorylcholine group of material surface, and graft modification is subject to the impact of base material composition, shape and equipment, limit it in the application in bio-medical material modification field and the further raising of biocompatibility.
Research group (Biomaterials, 2009,30 (28): 4930-4938 of Japan Ishihara and Britain Lewis; Biomaterials, 2004,25 (19): 4785-4796) protein adsorption and cell adhesion with the polymer-modified material surface of Phosphorylcholine that contains cation, neutrality, anion are studied.Result shows, charged Phosphorylcholine base polymer all can be used for improving the biocompatibility of material.The imitating cell outer-layer membrane structure coating that the Phosphorylcholine class binary of kayexalate/polypropylene amine hydrochlorate (PSS/PAH) multilayer film and outermost layer oppositely charged builds by Electrostatic Absorption with organic polymer; all significantly improve the biocompatibility issues such as protein adsorption, the cell adhesion (Langmuir on electrostatic self-assembled coating surface; 2009,25 (6): 3610-3617; Soft Matter, 2010,6 (7): 1503-1512).
With methylacryoyloxyethyl Phosphorylcholine-butyl methacrylate bipolymer (poly (MPC-co-BM A), polylactic acid (PLA) nanoparticle surface of PMB30) modifying, can suppress the absorption to albumen, it is difficult for by macrophage phagocytic, reach improve drug effect effect (intelligent macromolecule material. Beijing: Chemical Industry Press, 2004,281-282).Waiting people quietly has been prepared into the randomcopolymer that contains Phosphorylcholine group to have had imitative membrane structure nano-micelle (number of patent application: 201010192087.9).The people such as Mansouri by the chitosan self-assembly system that contains Phosphorylcholine group for wrapping up erythrocyte, effectively avoid erythrocyte by antibody A identification, haemolysis, improve its biocompatibility (Langmuir, 2009,25 (24): 14071-14078).The surperficial directly permeates cell membranes of nano-particle prepared by Phosphorylcholine polymer that contains is found in the people such as Goda (Biomaterials, 2010,31 (8): 2380-2387) research, has opened up new approach for improving drug effect.
Summary of the invention
Technical problem to be solved by this invention is for above-mentioned the deficiencies in the prior art, and a kind of imitating cell outer-layer membrane structure genophore is provided.This imitating cell outer-layer membrane structure genophore is made up by solution free radical polymerization method of the vinyl monomer that contains amphion hydrophilic radical, phenyl amines monomer and polycation, protein adsorption, platelet adhesion reaction are obviously reduced, biocompatibility significantly improves, thereby extending structure gene circulation time in vivo, improves drug effect.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of imitating cell outer-layer membrane structure genophore, be made up by radical polymerization of monomer and polycation, it is characterized in that, described monomer is vinyl monomer and the phenyl amines monomer that contains amphion hydrophilic radical; Described polycation is chitosan, polymine, polylysine or branch polyurethane; Described phenyl amines monomer is aniline or p-phenylenediamine (PPD).
Above-mentioned a kind of imitating cell outer-layer membrane structure genophore, described vinyl monomer is methacrylic, acrylic compounds, methacryl amine or acrylamide monomers.
Above-mentioned a kind of imitating cell outer-layer membrane structure genophore, described amphion hydrophilic radical is Phosphorylcholine group.
Above-mentioned a kind of imitating cell outer-layer membrane structure genophore, the quality of described monomer is 60%～70% of monomer and polycation gross mass; In described monomer, contain the vinyl monomer of amphion hydrophilic radical and the mass ratio of phenyl amines monomer is 2～8:1.
Above-mentioned a kind of imitating cell outer-layer membrane structure genophore, described in contain amphion hydrophilic radical vinyl monomer be methylacryoyloxyethyl Phosphorylcholine, phenyl amines monomer is aniline, polycation is chitosan.
In addition, the present invention also provides a kind of method of preparing above-mentioned imitating cell outer-layer membrane structure genophore, it is characterized in that, the method is: polycation is dissolved in solvent, obtains said polycation solution; Then in described said polycation solution, add monomer and initiator, under nitrogen protection, carry out polyreaction; After polyreaction finishes, reaction system is carried out to sucking filtration and obtain filtrate, regulate filtrate pH value to 5.5 rear centrifugal, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore.
Above-mentioned method, described solvent is that concentration of volume percent is 1%～5% aqueous acetic acid.
Above-mentioned method, described initiator is potassium peroxydisulfate, the quality of initiator is 0.5%～2% of monomer and polycation gross mass.
Above-mentioned method, the reaction temperature of described polyreaction is 60 DEG C～80 DEG C, the response time is 3h～5h.
The present invention compared with prior art has the following advantages:
1, imitating cell outer-layer membrane structure genophore of the present invention is made up by solution free radical polymerization method of the vinyl monomer that contains amphion hydrophilic radical, phenyl amines monomer and polycation, protein adsorption, platelet adhesion reaction are obviously reduced, biocompatibility significantly improves, thereby extending structure gene circulation time in vivo, improves drug effect.
2, the particle diameter of imitating cell outer-layer membrane structure genophore of the present invention is less, is about 300nm～600nm.
3, imitating cell outer-layer membrane structure genophore of the present invention surface Zeta potential is higher, can significantly improve its load capacity to DNA.
4, simple, the mild condition of the preparation method of imitating cell outer-layer membrane structure genophore of the present invention, the genophore system for obtaining surface with imitating cell outer-layer membrane structure provides a kind of new approach.
5, imitating cell outer-layer membrane structure genophore of the present invention has huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
Below in conjunction with drawings and Examples, technical scheme of the present invention is described in further detail.
Brief description of the drawings
Fig. 1 is the particle size distribution figure of the imitating cell outer-layer membrane structure genophore prepared of the embodiment of the present invention 1.
Fig. 2 is the impact of pH value imitating cell outer-layer membrane structure genophore mean diameter prepared by the embodiment of the present invention 1.
Fig. 3 is the impact of pH value imitating cell outer-layer membrane structure genophore surface Zeta potential prepared by the embodiment of the present invention 1.
Detailed description of the invention
MPC can be by the method for bibliographical information (Polymer Journal, 1990,22 (5): 355-360; Colloids and Surfaces B:Biointerfaces, 2011,85 (1): 48-55) synthetic.Aniline (AN) Deng Junkecong domestic production company buys or Sigma company buys.
Embodiment 1
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by 2-methylacryoyloxyethyl Phosphorylcholine (MPC), aniline and branch polyurethane, wherein the quality of monomer (2-methylacryoyloxyethyl Phosphorylcholine and aniline) is 60% of monomer and polycation (branch polyurethane) gross mass, and the mass ratio of 2-methylacryoyloxyethyl Phosphorylcholine and aniline is 2:1.
The preparation method of the present embodiment is: it is in 1% aqueous acetic acid that 0.1g branch polyurethane is dissolved in to 50mL concentration of volume percent, stirs 24h, obtains branch polyurethane solution; Then to the potassium peroxydisulfate (K that adds 0.1g2-methylacryoyloxyethyl Phosphorylcholine (MPC), 0.05g aniline and 5mg in branch polyurethane solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 4h under the condition of 70 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 380nm～600nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by 2-methylacryoyloxyethyl Phosphorylcholine (MPC), aniline and chitosan, wherein the quality of monomer (2-methylacryoyloxyethyl Phosphorylcholine and aniline) is 65% of monomer and polycation (chitosan) gross mass, and the mass ratio of 2-methylacryoyloxyethyl Phosphorylcholine and aniline is 4:1.
The preparation method of the present embodiment is: it is in 5% aqueous acetic acid that 0.35g chitosan is dissolved in to 50mL concentration of volume percent, stirs 24h, obtains chitosan solution; Then to the potassium peroxydisulfate (K that adds 0.52g2-methylacryoyloxyethyl Phosphorylcholine, 0.13g aniline and 0.02g in chitosan solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 3h under the condition of 80 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 300nm～500nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
Embodiment 3
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by 2-methylacryoyloxyethyl Phosphorylcholine (MPC), aniline and chitosan, wherein the quality of monomer (2-methylacryoyloxyethyl Phosphorylcholine and aniline) is 70% of monomer and polycation (chitosan) gross mass, and the mass ratio of 2-methylacryoyloxyethyl Phosphorylcholine and aniline is 6:1.
The preparation method of the present embodiment is: it is in 3% aqueous acetic acid that 0.3g chitosan is dissolved in to 50mL concentration of volume percent, stirs 12h, obtains chitosan solution; Then to the potassium peroxydisulfate (K that adds 0.6g2-methylacryoyloxyethyl Phosphorylcholine (MPC), 0.1g aniline and 5mg in chitosan solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 3h under the condition of 70 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 400nm～600nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by acrylyl oxy-ethyl Phosphorylcholine, aniline and polylysine, wherein the quality of monomer (acrylyl oxy-ethyl Phosphorylcholine and aniline) is 60% of monomer and polycation (polylysine) gross mass, and the mass ratio of acrylyl oxy-ethyl Phosphorylcholine and aniline is 2:1.
The preparation method of the present embodiment is: it is in 1% aqueous acetic acid that 0.2g polylysine is dissolved in to 50mL concentration of volume percent, stirs 15h, obtains polylysine solution; Then to the potassium peroxydisulfate (K that adds 0.2g acrylyl oxy-ethyl Phosphorylcholine, 0.1g aniline and 5mg in polylysine solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 5h under the condition of 60 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 400nm～550nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by 2-methylacryoyloxyethyl Phosphorylcholine (MPC), p-phenylenediamine (PPD) and polymine, wherein the quality of monomer (2-methylacryoyloxyethyl Phosphorylcholine and p-phenylenediamine (PPD)) is 63% of monomer and polycation (polymine) gross mass, and the mass ratio of 2-methylacryoyloxyethyl Phosphorylcholine and p-phenylenediamine (PPD) is 8:1.
The preparation method of the present embodiment is: it is in 3% aqueous acetic acid that 0.37g polymine is dissolved in to 50mL concentration of volume percent, stirs 24h, obtains polyethyleneimine: amine aqueous solution; Then to the potassium peroxydisulfate (K that adds 0.56g2-methylacryoyloxyethyl Phosphorylcholine, 0.07g p-phenylenediamine (PPD) and 0.02g in polyethyleneimine: amine aqueous solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 4h under the condition of 70 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 400nm～600nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
The imitating cell outer-layer membrane structure genophore of the present embodiment, made by radical polymerization by 2-methylacryoyloxyethyl Phosphorylcholine (MPC), p-phenylenediamine (PPD) and chitosan, wherein the quality of monomer (2-methylacryoyloxyethyl Phosphorylcholine and p-phenylenediamine (PPD)) is 70% of monomer and polycation (chitosan) gross mass, and the mass ratio of 2-methylacryoyloxyethyl Phosphorylcholine and p-phenylenediamine (PPD) is 6:1.
The preparation method of the present embodiment is: it is in 5% aqueous acetic acid that 0.3g chitosan is dissolved in to 50mL concentration of volume percent, stirs 20h, obtains chitosan solution; Then to the potassium peroxydisulfate (K that adds 0.6g2-methylacryoyloxyethyl Phosphorylcholine, 0.1g p-phenylenediamine (PPD) and 0.01g in chitosan solution
2s
2o
8, KPS), under nitrogen protection, temperature is polyreaction 3h under the condition of 80 DEG C; After polyreaction finishes, with G3 funnel, reaction system is carried out to sucking filtration and obtain filtrate, regulate after filtrate pH value to 5.5 centrifugal 10min under 2 DEG C～4 DEG C, 9000rpm condition, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization at-50 DEG C after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore, its particle size range is about 290nm～500nm.
The imitating cell outer-layer membrane structure genophore of the present embodiment obviously reduces protein adsorption, platelet adhesion reaction, biocompatibility significantly improves, thereby extend genophore circulation time in vivo, improve drug effect, there is huge learning value and wide application prospect in fields such as organizational project, medicine controlled releasing, gene therapy and biosensors.
The particle size distribution of imitating cell outer-layer membrane structure genophore prepared by the embodiment of the present invention 1 to embodiment 6 detects, and the results are shown in Figure 1, and as can be seen from the figure, the particle diameter of imitating cell outer-layer membrane structure genophore of the present invention is less, is about 300nm～600nm.
Under condition of different pH, particle diameter and the Zeta potential of imitating cell outer-layer membrane structure genophore prepared by the embodiment of the present invention 1 detect, and result is shown in respectively Fig. 2 and Fig. 3.As can be seen from Figure 2, the particle diameter of imitating cell outer-layer membrane structure genophore reduces gradually with the rising of pH value, but still maintains within the scope of 300nm～600nm.As can be seen from Figure 3, under acid condition, imitating cell outer-layer membrane structure genophore keeps higher Zeta potential, Zeta potential reaches as high as 42mV, under the condition of pH=4.5, its Zeta potential reaches 38mV left and right, is 201210415034.8 apparently higher than application number, and denomination of invention is the Zeta potential of the patent application of " having nano-particle of imitating cell outer-layer membrane structure and preparation method thereof ".
Be 201210415034.8 by application number, it is 4.5 solution that genophore prepared by nano-particle prepared by the embodiment 1 that denomination of invention is the patent application of " having nano-particle of imitating cell outer-layer membrane structure and preparation method thereof " and the embodiment of the present invention 1 to embodiment 6 is mixed with respectively isocyatic pH value, with the Zeta potential of dynamic light scattering test solution at 25.0 DEG C, get the meansigma methods of measuring for 15 times, the results are shown in following table:
The Zeta potential (pH=4.5) on table 1 nano-particle and genophore surface
| Sample | Zeta potential (mV) |
| Nano-particle (application number 201210415034.8) | 18.3±0.4 |
| The embodiment of the present invention 1 | 38±0.5 |
| The embodiment of the |
37±0.3 |
| The embodiment of the present invention 3 | 50±0.7 |
| The embodiment of the |
42±0.6 |
| The embodiment of the |
45±0.5 |
| The embodiment of the |
41±0.3 |
As can be seen from Table 1, it is 201210415034.8 that the Zeta potential of imitating cell outer-layer membrane structure genophore of the present invention reaches application number, more than the twice of the Zeta potential of the patent application that denomination of invention is " having nano-particle of imitating cell outer-layer membrane structure and preparation method thereof ", can significantly improve its load capacity to DNA.
The above; it is only preferred embodiment of the present invention; not the present invention is imposed any restrictions, every any simple modification of above embodiment being done according to the technology of the present invention essence, change and equivalent structure change, and all still belong in the protection domain of technical solution of the present invention.
Claims (9)
1. an imitating cell outer-layer membrane structure genophore, is made up by radical polymerization of monomer and polycation, it is characterized in that, described monomer is vinyl monomer and the phenyl amines monomer that contains amphion hydrophilic radical; Described polycation is chitosan, polymine, polylysine or branch polyurethane; Described phenyl amines monomer is aniline or p-phenylenediamine (PPD).
2. a kind of imitating cell outer-layer membrane structure genophore according to claim 1, is characterized in that, described vinyl monomer is methacrylic, acrylic compounds, methacryl amine or acrylamide monomers.
3. a kind of imitating cell outer-layer membrane structure genophore according to claim 1, is characterized in that, described amphion hydrophilic radical is Phosphorylcholine group.
4. a kind of imitating cell outer-layer membrane structure genophore according to claim 1, is characterized in that, the quality of described monomer is 60%～70% of monomer and polycation gross mass; In described monomer, contain the vinyl monomer of amphion hydrophilic radical and the mass ratio of phenyl amines monomer for (2～8): 1.
5. a kind of imitating cell outer-layer membrane structure genophore according to claim 1, it is characterized in that, the described vinyl monomer that contains amphion hydrophilic radical is methylacryoyloxyethyl Phosphorylcholine, and phenyl amines monomer is aniline, and polycation is chitosan.
6. a method of preparing the imitating cell outer-layer membrane structure genophore as described in arbitrary claim in claim 1 to 5, is characterized in that, the method is: polycation is dissolved in solvent, obtains said polycation solution; Then in described said polycation solution, add monomer and initiator, under nitrogen protection, carry out polyreaction; After polyreaction finishes, reaction system is carried out to sucking filtration and obtain filtrate, regulate filtrate pH value to 5.5 rear centrifugal, supernatant discarded, to adding distilled water centrifugalize three times repeatedly in the solid after centrifugal, by the solid lyophilization after centrifugalize repeatedly, obtain imitating cell outer-layer membrane structure genophore.
7. method according to claim 6, is characterized in that, described solvent is that concentration of volume percent is 1%～5% aqueous acetic acid.
8. method according to claim 6, is characterized in that, described initiator is potassium peroxydisulfate, and the quality of initiator is 0.5%～2% of monomer and polycation gross mass.
9. method according to claim 6, is characterized in that, the reaction temperature of described polyreaction is 60 DEG C～80 DEG C, and the response time is 3h～5h.
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