CN103868942B - Semiconductor microactuator nanostructured photoproduction active oxygen species method for qualitative analysis - Google Patents
Semiconductor microactuator nanostructured photoproduction active oxygen species method for qualitative analysis Download PDFInfo
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Abstract
A kind of method that the invention discloses qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species.Comprise the following steps: semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO and deionized water mix the feature ESR spectrum A after utilizing electron spin resonance spectrometer to obtain irradiation；Granule to be checked, BMPO, superoxide dismutase SOD and deionized water are mixed to get the spectrum B after irradiation；Relatively ESR spectrum A, B, obtain the qualitative analysis of superoxide radical and hydroxyl radical free radical；Granule to be checked, spin trapping agent TEMP and deionized water are mixed to get spectrum C；Granule to be checked, TEMP, NaN3It is mixed to get spectrum D with deionized water；Relatively ESR spectrum C, D, obtain the qualitative analysis of single line oxygen.The method, in conjunction with Direct Acquisition technology and indirect clearance technique, distinguishes the kind of active oxygen species more accurately, particularly to the system producing different activities oxygen species simultaneously, compensate for the deficiency of conventional chemical luminescence method and fluorescence method.
Description
Technical field
A kind of method that the present invention relates to qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species.
Background technology
Semiconductor microactuator nanostructured is because of its superior optical electrical catalysis characteristics, getting more and more in environmental improvement and the field such as energy conversion and utilization on the one hand and cause the attention of scientific research and industrial quarters, this also excites the great interest of optical physics and photochemical mechanism comparison in scientists study conductor photocatalysis activity.But then, a large amount of uses of semiconductor microactuator nano-particle inevitably will cause new environment, biological and healthy and safe crisis, the safety evaluatio of these nano-particle is then needed to be grasped to the reaction mechanism of semiconductive light active.Light excites the generation of hole/electronics pair and electrodes transfer behaviour to be considered as the key factor determining semiconductor nanoparticle induced chemical reaction.As the possible product of electronics and hole, active oxygen species is species in the middle of the typical activity in conductor photocatalysis course of reaction.Active oxygen species (ReactiveOxygenSpecies, ROS), refers to one group of significantly high oxygen-containing molecules of chemism or species, mainly includes hydroxyl radical free radical (OH), Superoxide radical anion (O2 -), single line oxygen (1O2) and hydrogen peroxide (H2O2) etc..In vivo, these active oxygen species play Signal Regulation and maintain the critical function that stress balance, the side-product of they usually a lot of biological aerobic metabolisies.Active oxygen species has very active chemical characteristic, and such as hydroxyl radical free radical oxidisability is extremely strong, it is possible to the biologically active structure of surrounding is caused badly damaged by non-selectivity.The oxidative stress excessively producing to cause of reactive oxygen free radical is considered as the main cause causing cell senescence and many cancers and neurodegenerative disease.Additionally, with nanoparticle size, pattern, valence band structure and crystal structure importance classes in nanometer semiconductor structure photolytic activity seemingly, the generation of active oxygen species is also regarded as determining semiconductive light active catalysis characteristics, antibacterial activity or Cytotoxic inherent important parameter active oxygen species.Therefore, a kind of method setting up variety classes active oxygen species discriminatory analysis that can be used in and producing in semiconductor microactuator nanostructured photoirradiation, it will have important effect for understanding semiconductive light active feature and design and safety evaluatio to new material.
Currently for the detection of active oxygen species, mainly there are three kinds of methods: chemiluminescence spectra method (CL), fluorescent spectrometry (FL) and electron spin resonance (Electronspinresonancespectroscopy, ESR)).Comparatively speaking, but chemiluminescence spectra method can simply measure the existence of hydroxyl radical free radical, superoxide radical and hydrogen peroxide can not accurately distinguish them.Fluorescence method is the method for the indirect detection ROS employed up to, it is possible to the relatively easy existence detecting ROS, but this method is highly susceptible to the interference of other active oxide materials and needs extra instrument such as HPLC to distinguish the kind determining ROS.Electron spin resonance is qualitative and quantitative assay short life free radical and ROS the most reliable, most straightforward approach at present in conjunction with spin trapping technology, has been widely used in the field such as biomedicine, archaeological chemistry.This method is to utilize spin trapping molecule and radical reaction to form a kind of metastable spin adduct, is then based on the feature ESR signal that this adduct has and carries out identification and the mensuration of ROS.But this method exists problem rigorous not, can frequently result in the accuracy to ROS detection and reduce.Such as same kind nano material TiO2, some bibliographical information TiO2Superoxide radical can be produced, and some documents are thought and can not be produced.
Summary of the invention
The present invention is directed to the deficiency of existing detection active oxygen species technology, it is provided that a kind of spin trapping-clearance technique combines the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species.
The present invention solves above-mentioned technical problem, the technical scheme of employing is as follows:
A kind of method of qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species, it includes:
Mixed solution A is obtained, the ESR spectrum A after utilizing electron spin resonance spectrometer to obtain photoirradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO and deionized water being mixed；Mixed solution B is obtained, the ESR spectrum B after utilizing electron spin resonance spectrometer to obtain photoirradiation after separately semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO, superoxide dismutase SOD and deionized water being mixed；By comparing ESR spectrum A, B, carry out the qualitative analysis of superoxide radical, hydroxyl radical free radical；
Mixed solution C is obtained, the ESR spectrum C after utilizing electron spin resonance spectrometer to obtain photoirradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP and deionized water being mixed；Another by semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP, NaN3Mixed solution D is obtained, the ESR spectrum D after utilizing electron spin resonance spectrometer to obtain photoirradiation after mixing with deionized water；By comparing ESR spectrum C, D, carry out the qualitative analysis of single line oxygen-derived free radicals.
By such scheme, described to superoxide radical, the qualitative analysis concrete grammar of hydroxyl radical free radical is as follows: if ESR spectrum A does not have feature ESR signal, represents that namely semiconductor microactuator nano-particle to be detected does not have superoxide radical to produce also without hydroxyl radical free radical；If ESR spectrum A creates the feature ESR signal of BMPO and superoxide radical spin addition product and ESR blackout in ESR spectrum B, then it represents that semiconductor microactuator nano-particle to be detected creates superoxide radical under thermal effect；If ESR spectrum A and ESR spectrum B is consistent, show the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then it represents that semiconductor microactuator nano-particle to be detected creates hydroxyl radical free radical under thermal effect；If ESR spectrum A has ESR signal, ESR spectrum B and A compares, ESR signal weaker, Spectral Characteristic changes, create the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then represent that semiconductor microactuator nano-particle to be detected had both created hydroxyl radical free radical under thermal effect, create again superoxide radical.
By such scheme, the concrete grammar that described single line oxygen activity species carry out qualitative analysis is as follows: if ESR spectrum C does not have ESR signal to produce, then it represents that semiconductor microactuator nano-particle to be detected does not have single line oxygen to produce；If creating the feature ESR spectrogram of TEMP and single line oxygen spin addition product in ESR spectrum C, and in ESR spectrum D, TEMP disappears with single line oxygen spin addition product feature ESR spectrogram, then it represents that semiconductor microactuator nano-particle to be detected creates single line oxygen.
By such scheme, in described mixed solution A, mixed solution B, mixed solution C and mixed solution D, the concentration of semiconductor microactuator nano-particle to be detected is 0.1mg/mL.
By such scheme, in described mixed solution A, the concentration of spin trapping agent BMPO is 25mmol/L.
By such scheme, in described mixed solution C, the concentration of spin trapping agent TEMP is 10mmol/L.
By such scheme, in described mixed solution B, the concentration of superoxide dismutase SOD is 4U/mL.
By such scheme, NaN in described mixed solution D3Concentration be 10mmol/L.
By such scheme, the light path system of described electron spin resonance spectrometer uses Newport light path system 450W xenon lamp to produce the light of more than wavelength 350nm as light source plus WG320 optical filter.
By such scheme, described electron spin resonance spectrometer running parameter is set to: microwave power 20mW, central magnetic field 3518.5G, sweep interval 100G, magnetic field modulation 1G, sweep time 80s, light application time 5min.
Spin trapping agent BMPO full name is 5-tert-butyl carbonyl-5-methyl isophthalic acid-pyrrolin-N-oxide (5-tert-butoxycarbonyl5-methyl-1-pyrrolineN-oxide), BMPO catches superoxide radical and the most commonly used spin trapping molecule of hydroxyl radical free radical, BMPO itself does not have ESR signal, but spin adduct can be formed with superoxide radical or hydroxyl radical reaction, show the ESR collection of illustrative plates of uniqueness.Superoxide dismutase (SOD) can remove superoxide radical specially as a kind of enzyme.
Spin trapping agent TEMP full name is 4-oxygen-2,2,6,6-tetramethyl piperidines (4-oxo-2,2,6,6-tetramethylpiperidine), and TEMP molecule is the conventional trapping agent catching single line oxygen species, and NaN3For removing the chemical molecular of single line oxygen specially.TEMP itself is without ESR signal, but it can with three line ESR spectrums of single line oxygen effect formation feature.
Beneficial effects of the present invention:
1) the method combines directly spin capture technique and indirect clearance technique, the kind of active oxygen species can be distinguished more exactly, particularly to the system producing different activities oxygen species simultaneously, the various active oxygen species realizing quasiconductor micro nano structure is produced under thermal effect measure respectively, compensate for the deficiency of conventional chemical luminescence method and fluorescence method.
2) test speed test fast, each only needs a few minutes to complete；Oxygen consumption test few, each only needs a few microliters of sample；
3) generation of various active oxygen species can be monitored in real time and have wide range of applications, except semiconductor microactuator nanostructured, it may also be used for other various nanostructureds produce the mensuration of active oxygen species and electronics.
Accompanying drawing explanation
The ESR collection of illustrative plates that Fig. 1, embodiment 1 carry out CdS micro nano structure superoxide radical, hydroxyl radical free radical is analyzed；
Fig. 2, embodiment 1 carry out CdS micro nano structure and carry out the ESR collection of illustrative plates of single line oxygen analysis；
The ESR collection of illustrative plates that Fig. 3, embodiment 2 carry out ZnS micro nano structure superoxide radical, hydroxyl radical free radical is analyzed；
Fig. 4, embodiment 2 carry out the ESR collection of illustrative plates that ZnS micro nano structure single line oxygen is analyzed；
Fig. 5, embodiment 3 carry out In2S3The ESR collection of illustrative plates that micro nano structure superoxide radical, hydroxyl radical free radical are analyzed.
Detailed description of the invention
For making those skilled in the art be more fully understood that technical scheme, explain technical scheme further below in conjunction with accompanying drawing and following example, but not as limiting the scope of the invention.
The method of spin trapping-clearance technique qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species:
Mixed solution A is obtained, the ESR spectrum A after utilizing electron spin resonance spectrometer to obtain irradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO and deionized water being mixed；Mixed solution B is obtained, the ESR spectrum B after utilizing electron spin resonance spectrometer to obtain irradiation after separately semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO, superoxide dismutase SOD and deionized water being mixed；By comparing ESR spectrum A, B, carry out the qualitative analysis of superoxide radical, hydroxyl radical free radical；
If ESR spectrum A does not have feature ESR signal, represent that namely semiconductor microactuator nano-particle to be detected does not have superoxide radical to produce also without hydroxyl radical free radical；If ESR spectrum A creates the feature ESR signal of BMPO and superoxide radical spin addition product and ESR blackout in ESR spectrum B, then it represents that semiconductor microactuator nano-particle to be detected creates superoxide radical under light radiation；If ESR spectrum A and ESR spectrum B is consistent, show the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then it represents that semiconductor microactuator nano-particle to be detected creates hydroxyl radical free radical under thermal effect；If ESR spectrum A has ESR signal, ESR spectrum B and A compares, ESR signal weaker, Spectral Characteristic changes, create the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then it represents that semiconductor microactuator nano-particle to be detected had both created hydroxyl radical free radical, created again superoxide radical.
This mainly make use of following principle: BMPO catches superoxide radical and the most commonly used spin trapping molecule of hydroxyl radical free radical, BMPO itself does not have ESR signal, but spin adduct can be formed with superoxide radical or hydroxyl radical reaction, show the ESR collection of illustrative plates of uniqueness.Superoxide dismutase (SOD) can remove superoxide radical specially as a kind of enzyme, and hydroxyl radical free radical is not then affected.Present invention Demonstration Method directly or indirectly accurately can analyze the presence or absence of semiconductor microactuator nanostructured photoproduction active oxygen species superoxide radical and hydroxyl radical free radical qualitatively.
Mixed solution C is obtained, the ESR spectrum C after utilizing electron spin resonance spectrometer to obtain photoirradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP and deionized water being mixed；Another by semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP, NaN3Mixed solution D is obtained, the ESR spectrum D after utilizing electron spin resonance spectrometer to obtain photoirradiation after mixing with deionized water；By comparing ESR spectrum C, D, carry out the qualitative analysis of single line oxygen-derived free radicals.
If ESR spectrum C does not have ESR signal to produce, then it represents that semiconductor microactuator nano-particle to be detected does not produce single line oxygen；If creating the feature ESR spectrogram of TEMP and single line oxygen spin addition product in ESR spectrum C, and in ESR spectrum D, TEMP disappears with single line oxygen spin addition product feature ESR spectrogram, then it represents that semiconductor microactuator nano-particle to be detected creates single line oxygen.
This mainly make use of following principle: spin trapping agent TEMP catches the spin trapping molecule that single line oxygen is the most commonly used, and TEMP itself does not have ESR signal, but can react formation spin adduct with single line oxygen, shows the ESR collection of illustrative plates of uniqueness.NaN3For removing the chemical molecular of single line oxygen specially.Present invention Demonstration Method directly or indirectly accurately can analyze the presence or absence of semiconductor microactuator nanostructured photoproduction active oxygen species single line oxygen qualitatively.
In the implementation process of the present invention, the embodiment that each concentration of component optimizes is as follows:
The concentration of semiconductor microactuator nano-particle to be detected is 0.1mg/mL.
Spin trapping agent BMPO and TEMP working concentration respectively 25mmol/L and 10mmol/L.
The working concentration of superoxide dismutase SOD is 4U/mL.
NaN3Working concentration be 10mmol/L.
Embodiment 1
The mensuration of CdS micro nano structure photoproduction active oxygen species, CdS micro-nano granules to be measured is 1.5 μm of hexagonal wurtzite structures.
Preparation: ESR instrument designing: ESR measures the BrukerEMX electron spin resonance spectrometer of Brooker company.Light source is the light of more than the 350nm that Newport light path system 450W xenon lamp produces plus WG320 optical filter.ESR test parameter is: temperature (room temperature), microwave power (20mW), central magnetic field (3518.5G), sweep interval (100G), magnetic field modulation (1G), sweep time (80s), light application time (0,1,3,5 and 8min).Configuration storing solution: 250mMBMPO, 100mMTEMP, 100mMNaN3, 40U/mLSOD, 1mg/mLCdS aqueous solution.
1) mensuration of superoxide radical: take the aqueous solution of 5 microlitre 1mg/mLCdS micro-nano granules, 5 microlitre 250mM spin trapping agent BMPO and 40 microliters of deionized water mix homogeneously, be transferred in the quartz capillary that internal diameter is 0.9mm, sealing.Being inserted by capillary tube in ESR test chamber, recording light is according to the ESR spectrum after front and illumination 5 minutes；
2) superoxide dismutase clean-up effect to superoxide radical: take the aqueous solution of 5 μ L1mg/mLCdS micro-nano granules, 5 microlitre 250mM spin trapping agent BMPO, 5 μ L40U/mLSOD and 35 microliters of deionized water mix homogeneously, it is transferred in the quartz capillary that internal diameter is 0.9mm, sealing.Capillary tube is inserted in ESR test chamber, recording light photograph ESR spectrum after 5 minutes；
3) when accompanying drawing 1 is adopt BMPO as spin trapping agent, CdS micro nano structure produces the feature ESR collection of illustrative plates of active oxygen species under illumination effect.BMPO catches superoxide radical and the most commonly used spin trapping molecule of hydroxyl radical free radical, and BMPO itself does not have ESR signal, but can form spin adduct with superoxide radical or hydroxyl radical reaction, show the ESR collection of illustrative plates of uniqueness.As can be seen from Figure 1, compared with control experiment, the sample containing CdS micro-nano granules produces very strong ESR signal under light illumination, and (its TuPu method is four line spectrums of relative intensity respectively 1:1:1:1, hyperfine splitting parameter is aN=13.4,aβ H=12.1G), this is the ESR collection of illustrative plates of typical BMPO/ OOH spin addition product, illustrates to produce superoxide radical under CdS illumination effect.Superoxide dismutase (SOD) can remove superoxide radical specially as a kind of enzyme.Fig. 1 shows, after adding SOD, the ESR signal that CdS produces is eliminated substantially, again shows that CdS generates superoxide radical.
4) mensuration of single line oxygen: take the aqueous solution of 5 μ L1mg/mLCdS micro-nano granules, 5 μ L100mM spin trapping agent TEMP and 40 μ L deionized water mix homogeneously, be transferred in the quartz capillary that internal diameter is 0.9mm, sealing.Being inserted by capillary tube in ESR test chamber, recording light is according to the ESR spectrum after front and illumination 5 minutes；
5) NaN3Scavenging action to single line oxygen: take the aqueous solution of 5 μ L1mg/mLCdS micro-nano granules, 5 μ L100mM spin trapping agent TEMP, 5 μ L100mMNaN3With 35 microliters of deionized water mix homogeneously, be transferred in the quartz capillary that internal diameter is 0.9mm, sealing.Capillary tube is inserted in ESR test chamber, recording light photograph ESR spectrum after 5 minutes.
6) accompanying drawing 2 measures CdS micro nano structure when being adopt TEMP as trapping agent and produces the ESR collection of illustrative plates of single line oxygen under illumination effect.TEMP molecule is the conventional trapping agent catching single line oxygen species, and NaN3For removing the chemical molecular of single line oxygen specially.TEMP itself is without ESR signal, but it can with three line ESR spectrums of single line oxygen effect formation feature.As shown in Figure 2, when creating strong ESR signal under illumination after TEMP and CdS mixes, the generation of single line oxygen is described.NaN3After addition, ESR signal is eliminated substantially, produces single line oxygen species under side light CdS illumination effect.
Embodiment 2
The mensuration of ZnS micro nano structure photoproduction active oxygen species, ZnS micro-nano granules to be measured is 2.0 μm of hexagonal wurtzite structures.
Repeating the operating procedure of embodiment 1, micro-nano granules to be detected changes above-mentioned ZnS micro-nano granules into.
With reference to accompanying drawing 3, adopt BMPO to measure ZnS micro nano structure as spin trapping agent and produce the feature ESR collection of illustrative plates of active oxygen species under light illumination.As can be seen from Figure 3, compared with control experiment, sample containing ZnS micro-nano granules produces very strong ESR signal under light illumination, and Spectral Characteristic relatively meets the feature ESR collection of illustrative plates of BMPO/ OOH, after adding SOD, ESR signal weaker, and Spectral Characteristic changes, occurs in that TuPu method be four line spectrums of relative intensity respectively 1:2:2:1, hyperfine splitting parameter is aN=13.56,aβ H=12.30,aγHThe ESR figure of=0.66G, this is the feature ESR signal of BMPO and hydroxyl radical free radical spin addition product (BMPO/ OH), in combination with showing that ZnS has been simultaneously generated superoxide radical and hydroxyl radical free radical.Wherein, the ESR From Spectral Signal of ZnS+BMPO illumination 5min more meets the feature of BMPO/ OOH, and to be primarily due to the former signal without the feature ESR signal demonstrating BMPO/ OH relatively strong, covers the Spectral Characteristic of the latter.Under illumination effect, the ESR collection of illustrative plates of single line oxygen is produced with reference to accompanying drawing 4, ZnS micro nano structure.As shown in Figure 4, all illustrate produce ZnS illumination effect single line oxygen species from Direct Acquisition and indirect result of removing.
Embodiment 3
In2S3The mensuration of micro nano structure photoproduction active oxygen species, In to be measured2S3Micro-nano granules is 4.0 μm of tetragonal crystal system indium sulfides.
Repeating the operating procedure of embodiment 1, micro-nano granules to be detected changes above-mentioned In into2S3Micro-nano granules.
With reference to accompanying drawing 5, In2S3Generation superoxide radical it is detected under illumination effect.But it does not have observe In2S3Produce the ESR signal of single line oxygen, the In prepared by surface2S3Micro nano structure can not produce single line oxygen.
Embodiment 4
Bi2S3The mensuration of micro nano structure photoproduction active oxygen species, Bi to be measured2S3Micro-nano granules is 2.0 μm of rhombic bismuthine structures.
Repeating the operating procedure of embodiment 1, micro-nano granules to be detected changes above-mentioned Bi into2S3Micro-nano granules.
Result shows, Bi2S3Micro-nano granules can not produce hydroxyl radical free radical, superoxide radical, can not produce single line oxygen.
Claims (8)
1. the method for a qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species, it is characterised in that comprise the following steps:
Mixed solution A is obtained, the ESR spectrum A after utilizing electron spin resonance spectrometer to obtain photoirradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO and deionized water being mixed；Mixed solution B is obtained, the ESR spectrum B after utilizing electron spin resonance spectrometer to obtain photoirradiation after separately semiconductor microactuator nano-particle to be detected, spin trapping agent BMPO, superoxide dismutase SOD and deionized water being mixed；By comparing ESR spectrum A, B, carry out the qualitative analysis of superoxide radical, hydroxyl radical free radical；
If ESR spectrum A does not have feature ESR signal, represent that namely semiconductor microactuator nano-particle to be detected does not have superoxide radical to produce also without hydroxyl radical free radical；If ESR spectrum A creates the feature ESR signal of BMPO and superoxide radical spin addition product and ESR blackout in ESR spectrum B, then it represents that semiconductor microactuator nano-particle to be detected creates superoxide radical under light radiation；If ESR spectrum A and ESR spectrum B is consistent, show the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then it represents that semiconductor microactuator nano-particle to be detected creates hydroxyl radical free radical under thermal effect；If ESR spectrum A has ESR signal, ESR spectrum B and A compares, ESR signal weaker, Spectral Characteristic changes, create the feature ESR spectrogram of BMPO and hydroxyl radical free radical spin addition product, then it represents that semiconductor microactuator nano-particle to be detected had both created hydroxyl radical free radical, created again superoxide radical；
Mixed solution C is obtained, the ESR spectrum C after utilizing electron spin resonance spectrometer to obtain photoirradiation after semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP and deionized water being mixed；Another by semiconductor microactuator nano-particle to be detected, spin trapping agent TEMP, NaN3Mixed solution D is obtained, the ESR spectrum D after utilizing electron spin resonance spectrometer to obtain photoirradiation after mixing with deionized water；By comparing ESR spectrum C, D, carry out the qualitative analysis of single line oxygen-derived free radicals；
If ESR spectrum C does not have ESR signal to produce, then it represents that semiconductor microactuator nano-particle to be detected does not have photoproduction active oxygen species single line oxygen；If creating the feature ESR spectrogram of TEMP and single line oxygen spin addition product in ESR spectrum C, and in ESR spectrum D, TEMP disappears with single line oxygen spin addition product feature ESR spectrogram, then it represents that semiconductor microactuator nano-particle to be detected creates single line oxygen.
2. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that the concentration of described semiconductor microactuator nano-particle to be detected is 0.1mg/mL.
3. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that in described mixed solution A, the concentration of spin trapping agent BMPO is 25mmol/L.
4. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that in described mixed solution C, the concentration of spin trapping agent TEMP is 10mmol/L.
5. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that in described mixed solution B, the concentration of superoxide dismutase SOD is 4U/mL.
6. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that NaN in described mixed solution D3Concentration be 10mmol/L.
7. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterised in that the light path system of described electron spin resonance spectrometer uses Newport light path system 450W xenon lamp to produce the light of more than wavelength 350nm as light source plus WG320 optical filter.
8. the method for qualitative analysis semiconductor microactuator nanostructured photoproduction active oxygen species as claimed in claim 1, it is characterized in that described electron spin resonance spectrometer running parameter is set to: microwave power 20mW, central magnetic field 3518.5G, sweep interval 100G, magnetic field modulation 1G, sweep time 80s, light application time 5min.
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| JPH06324132A (en) * | 1993-05-12 | 1994-11-25 | Katsumasa Koike | Method for forming water-soluble oxidized lipid and method for confirming and measuring oxidized lipid |
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| JPH06324132A (en) * | 1993-05-12 | 1994-11-25 | Katsumasa Koike | Method for forming water-soluble oxidized lipid and method for confirming and measuring oxidized lipid |
| CN101565453A (en) * | 2008-04-22 | 2009-10-28 | 中国科学院化学研究所 | Nitrone compound capable of capturing free radicals, preparation method thereof and use thereof |
| CN101525342A (en) * | 2009-04-03 | 2009-09-09 | 中国科学院化学研究所 | Surface self-assembly gold nanoprobe with free radical capture performance and preparing method and application thereof |
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