CN103865837A - Streptomyces having antagonistic effect on penicillium viridicatum - Google Patents
Streptomyces having antagonistic effect on penicillium viridicatum Download PDFInfo
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- CN103865837A CN103865837A CN201310712781.2A CN201310712781A CN103865837A CN 103865837 A CN103865837 A CN 103865837A CN 201310712781 A CN201310712781 A CN 201310712781A CN 103865837 A CN103865837 A CN 103865837A
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- jxnu02
- streptomyces
- penicillium viridicatum
- streptomycete
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Abstract
The invention relates to Streptomyces having antagonistic effect on penicillium viridicatum. The bacterial strain disclosed by the invention is preserved in a preservation authority appointed by national intellectual property office; the preservation authority is a typical culture collection center in China; the preservation date is 18th, November 2013; the preservation number is CCTCCNO:M2013575. The Streptomyces is identified to be Streptomyces anandii named treptomyces anandii JXNU02, and the Latin name is StreptomycesanandJXNU02. The Streptomyces anandii JXNU02 fermenting liquid has strong inhibition action on penicillium viridicatum, is stable in performance, and has good application value for development of new biological preservatives.
Description
Technical field
The present invention relates to microbial technology field, particularly relate to a kind of streptomycete that penicillium viridicatum is had to antagonistic action.
Background technology
Penicillium viridicatum (
penicillium viridicatum) belong to Penicillium fungi, growth and breeding in the food maturations such as the cereal of being everlasting, fruit, vegetables, nut and meat and storage.Penicillium viridicatum easily produces multiple mycotoxins in growth and breeding process, as ochratoxin A (ochratoxin A, OTA).OTA has strong carinogenicity and teratogenecity, kidney and liver is had to grievous injury simultaneously, is one of food primary pollution source, and human health and animal farming industry are had to great harm.Therefore the control of penicillium viridicatum is subject to scholar's extensive concern.
Go bad because food is vulnerable to the infringement of various harmful microbes in the process of processing, storage, consumption, food antiseptic is fresh-keeping has just become the major issue in foodstuffs industry.Along with the raising of people's living standard, and the enhancing of environmental consciousness, traditional chemical preservative has been proved has potential hazardness.Therefore, develop efficient, nontoxic, safe bio-preservative imperative.
Summary of the invention
The object of the present invention is to provide and there is antifungic action, particularly penicillium viridicatum is had the streptomycete of antagonistic action.
Realize technical scheme of the present invention as follows: the streptomycete that penicillium viridicatum is had to antagonistic action of the present invention is that the soil sample gathering from suburb, Nanchang City of Jiangxi Province prepares through separating, and its formal name used at school is A Nandeshi streptomycete JXNU02(
streptomyces anandiijXNU02).
The bacterial strain of the streptomycete that penicillium viridicatum is had to antagonistic action of the present invention has been kept at depositary institution's preservation that State Intellectual Property Office specifies, and depositary institution is Chinese Typical Representative culture collection center, and preservation address is Wuhan, China, Wuhan University; Preservation date is on November 18th, 2013, and deposit number is CCTCC NO:M 2013575.
The morphological specificity of A Nandeshi streptomycete JXNU02 bacterial strain:
Substrate mycelium physically well develops, without tabula, do not rupture; Aerial hyphae is at ISP2 substratum (yeast extract 4 g, malt extract 10 g, glucose 4 g, agar 20 g, distilled water 1 L, pH 7.3) upper well-grown, multi-branched, can form spore chain, fibrillae of spores twist, spore ellipse, smooth surface (seeing Fig. 1).
The physiological and biochemical property of A Nandeshi streptomycete JXNU02 bacterial strain:
A Nandeshi streptomycete JXNU02 can make Starch Hydrolysis, can produce hydrogen sulfide, can utilize tyrosine to produce melanochrome, can make gelatine liquefication, can make nitrate reduction, can not decomposition of cellulose, can not make milk peptonize; Glucose, sucrose, D-Fructose, L-arabinose, D-wood sugar, raffinose, inositol and PEARLITOL 25C can be utilized, but L-rhamnosyl can not be utilized; Cell walls type belongs to I type, in full cell hydrolyzed solution, contains ribose, does not detect characteristic sugar.
Penicillium viridicatum is had to the mensuration of the A Nandeshi streptomycete JXNU02 fermented liquid antimicrobial spectrum of antagonistic action.
Adopt agar diffusion method to measure A Nandeshi streptomycete JXNU02 fermented liquid antimicrobial spectrum.The target bacterium adopting is respectively penicillium viridicatum, Penicillium citrinum, yeast, Candida albicans, rice blast fungus, aspergillus niger, Mucor, cotton-wilt fusarium, Fusarium oxysporum, Rhizoctonia solani Kuhn, Rhizopus oryzae, sclerotinite, streptococcus aureus and intestinal bacteria.Result shows, A Nandeshi streptomycete JXNU02 fermenation raw liquid all has restraining effect to above-mentioned 13 kinds of target bacterium.
The impact of A Nandeshi streptomycete JXNU02 fermented liquid on the growth of penicillium viridicatum mycelia
A Nandeshi streptomycete JXNU02 fermented liquid can suppress the mycelial growth of penicillium viridicatum completely, and inhibiting rate reaches 100%, and result is as Fig. 2.
Beneficial effect of the present invention: the strain the present invention relates to has the bacterial strain of antagonistic action to penicillium viridicatum, and to the evaluation of classifying of this bacterial strain, determine that it is A Nandeshi streptomycete JXNU02(
streptomyces anandiijXNU02); Antimicrobial spectrum to A Nandeshi streptomycete JXNU02 fermented liquid is studied, and determines that it all has restraining effect to multiple fungi and bacterium; A Nandeshi streptomycete JXNU02 fermented liquid is studied the impact of penicillium viridicatum mycelia growth, determine that its fermented liquid suppresses the growth of penicillium viridicatum mycelia, and inhibiting rate reaches 100%.These data will lay the foundation for the development and application of foodstuffs biological preservative from now on.
Accompanying drawing explanation
Fig. 1 A Nandeshi streptomycete JXNU02 mycelia and spore shape (X10000).
The impact (A be fermented liquid, B be blank) of Fig. 2 A Nandeshi streptomycete JXNU02 fermented liquid on the growth of penicillium viridicatum mycelia.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated
The soil sample that embodiment 1 gathers from suburb, Nanchang City of Jiangxi Province, separates by the following method, cultivates and can obtain A Nandeshi streptomycete JXNU02, and adopting the method for shaking table liquid fermentation and culture to obtain has restraining effect fermented liquid to penicillium viridicatum.
(1) screening of streptomycete, preservation
The soil sample gathering from suburb, Nanchang City of Jiangxi Province, soil sample natural air drying, grinding is sieved, and 121 ℃ of dry heat treatment 1 h take 2 g soil in 18 ml sterile physiological water, 15 min that vibrate under 150 r/min conditions, then carry out gradient dilution, by 10
-2, 10
-3soil supension is coated respectively containing 75 mg/L K
2cr
2o
7gause I substratum (Zulkovsky starch 20 g, KNO
31 g, NaCl 0.5 g, K
2hPO
43H
2o 0.5 g, MgSO
47H
2o 0.5 g, FeSO
47H
2o 0.01g, agar powder 20g, distilled water 1 L, pH 7.4 ~ 7.6) on flat board, cultivate 7-14 d, bacterium colony separation and purification on Gause I culture medium flat plate that picking colony form is different for 28 ℃, purifying culture is inoculated in to Gause I inclined-plane, and for subsequent use in 4 ℃ of preservations.
Adopt dull and stereotyped face-off method primary dcreening operation Antagonistic Actinomycetes, with potato dextrose agar (potato 200g, glucose 20g, agar 20g, water 1 L) dull and stereotyped center is initial point, draws " ten ", be that 5 mm actinomycetes bacterium pieces are positioned over 2.5 cm places, anomaly plate center by diameter, cultivate after 2 d for 28 ℃, then the penicillium viridicatum piece that is 5 mm by diameter is inoculated in dull and stereotyped central authorities, observations after 28 ℃ of cultivation 5 d.
Adopt cup-plate method to sieve again Antagonistic Actinomycetes, take penicillium viridicatum as target bacterium, the bacterial strain that can produce inhibition zone is Antagonistic Actinomycetes, and adopts right-angled intersection method to measure antibacterial circle diameter with vernier callipers.
(2) preparation of A Nandeshi streptomycete JXNU02 fermented liquid
A Nandeshi streptomycete is seeded on Gause I flat board and cultivates after 7 d, gets 4 diameters, 5 mm actinomycetes bacterium cakes and is placed in seed culture medium (sucrose 22.5 g, soybean cake powder 12.5 g, K
2hPO
43H
2o 0.2 g, NaCl 1 g, Na
2sO
40.1 g, FeSO
47H
2o 0.01 g, CaCO
32 g, distilled water 1 L, pH 7.2), liquid amount 50 mL/ 250 mL triangular flasks are cultivated 48 h, i.e. seed liquor under 200 r/min, 28 ℃ of conditions.With 6 %(v/v) inoculum size in 50 mL/250 mL fermention mediums (Zulkovsky starch 10 g, soybean cake powder 15 g, KH
2pO
40.3 g, MgSO
47H
2o 0.3 g, distilled water 1 L, pH 7.2), under 200 r/min, 28 ℃ of conditions, cultivate 5 d.Fermented liquid is centrifugal 20 min under 10000 r/min conditions, collect supernatant liquor, and adopt 0.22 μ m membrane filtration degerming, are placed in 4 ℃ of preservations for subsequent use without fermented liquid.
(3) mensuration of A Nandeshi streptomycete JXNU02 fermented liquid antimicrobial spectrum
With appropriate stroke-physiological saline solution, the slant activation culture of target bacterium is suspended, obtain cell suspending liquid, control cell concn is 1X10
6cFU/mL.Get this bacterium suspension 15 μ l and join in the substratum that 7 mL are chilled to 50 ℃ of left and right in advance, after fully mixing, be laid on the agar bottom platform (diameter 15 mm) of 20 mL 2%.After condensing, place aseptic Oxford cup, then add 200 μ l fermented liquids, cultivate 24 h(bacteriums for 37 ℃) or 28 ℃ of cultivation 48 h(fungies), surveying antibacterial circle diameter is antibiotic activity.
(4) impact of A Nandeshi streptomycete JXNU02 fermented liquid on the growth of penicillium viridicatum mycelia
Measure respectively 1 mL A Nandeshi streptomycete JXNU02 fermented liquid and physiological saline (blank), add 10 mL to be chilled in advance in the potato dextrose agar of 50 ℃ of left and right, after fully mixing, pour in culture dish, the penicillium viridicatum piece that by diameter is again 5 mm is inoculated in dull and stereotyped central authorities, observations after 28 ℃ of cultivation 5 d.
CGCGCGGCGCGTGCTTACCATGCAGTCGAACGATGAACCACTTCGGTGGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATATGAGCCGCCCAGGCATCTGGGTGGCTGTAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAACGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCGCGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGGTGTGGGCAACATTCCACGTTGTCCGTGCCGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCCGTGTTGCCAGCAGGCCCTTGTGGTGCTGGGGACTCACGGGAGACCGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGAGCTGTCGAATGGTGTAAGCTGCAGGTCC。
Claims (3)
1. a streptomycete that penicillium viridicatum is had to antagonistic action, is characterized in that, its called after A Nandeshi streptomycete JXNU02(
streptomyces anandiijXNU02), this bacterial strain has been kept at depositary institution's preservation that State Intellectual Property Office specifies, and depositary institution is Chinese Typical Representative culture collection center, and preservation date is on November 18th, 2013, and deposit number is CCTCC NO:M 2013575.
2. as described in claim 1, penicillium viridicatum is had to the streptomycete bacterial strain of antagonistic action, it is characterized in that, its 16S rRNA gene order is:
CGCGCGGCGCGTGCTTACCATGCAGTCGAACGATGAACCACTTCGGTGGGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTGCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATATGAGCCGCCCAGGCATCTGGGTGGCTGTAAAGCTCCGGCGGTGCAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGAGGTAACGGCTCACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGAGAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCGCGTCGGTTGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCAGTCGATACGGGCAGGCTAGAGTTCGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCGATACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGGTGGGCACTAGGTGTGGGCAACATTCCACGTTGTCCGTGCCGCAGCTAACGCATTAAGTGCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATACACCGGAAAACCCTGGAGACAGGGTCCCCCTTGTGGTCGGTGTACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCCGTGTTGCCAGCAGGCCCTTGTGGTGCTGGGGACTCACGGGAGACCGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATACCGCGAGGTGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTTGTGGGAGGGAGCTGTCGAATGGTGTAAGCTGCAGGTCC。
3. as described in claim 1, penicillium viridicatum is had to the streptomycete bacterial strain of antagonistic action, it is characterized in that, it is for the control of penicillium viridicatum.
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|---|---|---|---|---|
| US11197457B2 (en) | 2015-05-01 | 2021-12-14 | Indigo Ag, Inc. | Designed complex endophyte compositions and methods for improved plant traits |
| EP3629742A4 (en) * | 2017-04-27 | 2022-01-05 | Flinders University Of South Australia | Bacterial inoculants |
| US11254908B2 (en) | 2013-12-24 | 2022-02-22 | Indigo Ag, Inc. | Plants containing beneficial endophytes |
| US11425912B2 (en) | 2014-06-20 | 2022-08-30 | The Flinders University Of South Australia | Inoculants and methods for use thereof |
| US11516989B2 (en) | 2017-03-01 | 2022-12-06 | Indigo Ag, Inc. | Endophyte compositions and methods for improvement of plant traits |
| US11570993B2 (en) | 2014-06-26 | 2023-02-07 | Indigo Ag, Inc. | Endophytes, associated compositions, and methods of use |
| US11747316B2 (en) | 2014-06-26 | 2023-09-05 | Ait Austrian Institute Of Technology Gmbh | Plant-endophyte combinations and uses therefor |
| US11753618B2 (en) | 2013-12-24 | 2023-09-12 | Indigo Ag, Inc. | Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds |
| US11751571B2 (en) | 2015-05-01 | 2023-09-12 | Indigo Ag, Inc. | Isolated complex endophyte compositions and methods for improved plant traits |
| US11751515B2 (en) | 2015-12-21 | 2023-09-12 | Indigo Ag, Inc. | Endophyte compositions and methods for improvement of plant traits in plants of agronomic importance |
| US11754553B2 (en) | 2013-09-04 | 2023-09-12 | Indigo Ag, Inc. | Agricultural endophyte-plant compositions, and methods of use |
| US11766045B2 (en) | 2016-12-01 | 2023-09-26 | Indigo Ag, Inc. | Modulated nutritional quality traits in seeds |
| US11771090B2 (en) | 2013-11-06 | 2023-10-03 | The Texas A&M Unversity System | Fungal endophytes for improved crop yields and protection from pests |
| US11793202B2 (en) | 2013-06-26 | 2023-10-24 | Indigo Ag, Inc. | Methods of use of seed-origin endophyte populations |
| US11807586B2 (en) | 2016-12-23 | 2023-11-07 | The Texas A&M University System | Fungal endophytes for improved crop yields and protection from pests |
| US11819027B2 (en) | 2015-06-08 | 2023-11-21 | Indigo Ag, Inc. | Streptomyces endophyte compositions and methods for improved agronomic traits in plants |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577985A (en) * | 2012-02-27 | 2012-07-18 | 武威市金绿源农业科技发展有限公司 | Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof |
-
2013
- 2013-12-23 CN CN201310712781.2A patent/CN103865837A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102577985A (en) * | 2012-02-27 | 2012-07-18 | 武威市金绿源农业科技发展有限公司 | Pig-raising fermentation bacterial agent for biological and environment-friendly fermentation bed and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| LI,L.M.,ET AL.: "GenBank accession number:KF595303.1", 《GENBANK》 * |
| 林虹等: "放线菌抗真菌活性筛选及其中一菌株的初步研究", 《暨南大学学报( 自然科学版)》 * |
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| US11793202B2 (en) | 2013-06-26 | 2023-10-24 | Indigo Ag, Inc. | Methods of use of seed-origin endophyte populations |
| US11754553B2 (en) | 2013-09-04 | 2023-09-12 | Indigo Ag, Inc. | Agricultural endophyte-plant compositions, and methods of use |
| US11771090B2 (en) | 2013-11-06 | 2023-10-03 | The Texas A&M Unversity System | Fungal endophytes for improved crop yields and protection from pests |
| US11753618B2 (en) | 2013-12-24 | 2023-09-12 | Indigo Ag, Inc. | Method for propagating microorganisms within plant bioreactors and stably storing microorganisms within agricultural seeds |
| US11254908B2 (en) | 2013-12-24 | 2022-02-22 | Indigo Ag, Inc. | Plants containing beneficial endophytes |
| US11445729B2 (en) | 2014-06-20 | 2022-09-20 | The Flinders University Of South Australia | Inoculants and methods for use thereof |
| US11425912B2 (en) | 2014-06-20 | 2022-08-30 | The Flinders University Of South Australia | Inoculants and methods for use thereof |
| US11570993B2 (en) | 2014-06-26 | 2023-02-07 | Indigo Ag, Inc. | Endophytes, associated compositions, and methods of use |
| US11747316B2 (en) | 2014-06-26 | 2023-09-05 | Ait Austrian Institute Of Technology Gmbh | Plant-endophyte combinations and uses therefor |
| US11751571B2 (en) | 2015-05-01 | 2023-09-12 | Indigo Ag, Inc. | Isolated complex endophyte compositions and methods for improved plant traits |
| US11197457B2 (en) | 2015-05-01 | 2021-12-14 | Indigo Ag, Inc. | Designed complex endophyte compositions and methods for improved plant traits |
| US11819027B2 (en) | 2015-06-08 | 2023-11-21 | Indigo Ag, Inc. | Streptomyces endophyte compositions and methods for improved agronomic traits in plants |
| US11751515B2 (en) | 2015-12-21 | 2023-09-12 | Indigo Ag, Inc. | Endophyte compositions and methods for improvement of plant traits in plants of agronomic importance |
| US11766045B2 (en) | 2016-12-01 | 2023-09-26 | Indigo Ag, Inc. | Modulated nutritional quality traits in seeds |
| US11807586B2 (en) | 2016-12-23 | 2023-11-07 | The Texas A&M University System | Fungal endophytes for improved crop yields and protection from pests |
| US11516989B2 (en) | 2017-03-01 | 2022-12-06 | Indigo Ag, Inc. | Endophyte compositions and methods for improvement of plant traits |
| EP3629742A4 (en) * | 2017-04-27 | 2022-01-05 | Flinders University Of South Australia | Bacterial inoculants |
| US11882838B2 (en) | 2017-04-27 | 2024-01-30 | The Flinders University Of South Australia | Bacterial inoculants |
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Application publication date: 20140618 |