CN103864767A - Puerarin refining process - Google Patents
Puerarin refining process Download PDFInfo
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- CN103864767A CN103864767A CN201410117256.0A CN201410117256A CN103864767A CN 103864767 A CN103864767 A CN 103864767A CN 201410117256 A CN201410117256 A CN 201410117256A CN 103864767 A CN103864767 A CN 103864767A
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- puerarin
- refining
- soluble
- glacial acetic
- acetic acid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D407/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00
- C07D407/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings
- C07D407/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having oxygen atoms as the only ring hetero atoms, not provided for by group C07D405/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
Abstract
The invention discloses a puerarin refining process comprising the steps of removing water-soluble, fat-soluble and acid-soluble impurities by using methanol and ethanol as dissolving agents of the puerarin, distilled water and glacial acetic acid as precipitants, and chloroform, anhydrous ether, distilled water and glacial acetic acid as impurity removal reagents; then removing impurities such as pigments and the like by using aluminum oxide and fly ash as stationary phases during column chromatography; finally refining the puerarin by using macro-porous adsorption resin. The puerarin refining process disclosed by the invention organically combines a variety of methods to purify the puerarin so as to ensure that the purity of the puerarin is more than 99%, and the yield of the puerarin is between 42% and 45%; the puerarin refining process can greatly reduce the production cost of enterprises, and has excellent social benefits and application prospects.
Description
Technical field
The invention belongs to pharmaceutical technology field, relate in particular to a kind of puerarin process for refining.
Background technology
Up to now, on domestic market, the visible drug product containing Radix Puerariae isoflavone has Radix Puerariae extractum preparation pill for curing apoplexy and relieving heart and the puerarin injection take puerarin as main component.Show according to Shandong medical courses in general institute clinical data, puerarin medicine series reaches 97.6% to cardiovascular and cerebrovascular diseases curative ratio, is cure difficult and complicated cases and lead to the new drug of morbidity, welcome by patient.At present domestic except Shandong, Shaanxi, Beijing, the built puerarin finished medicines production line take Radix Puerariae extract as raw material in Guangzhou, some larger pharmacy groups are good pueraria root series product one after another, along with the research that deepens continuously to root of kudzu vine pharmaceutical use, come out having more medicines containing kudzu vine root.The method of puerarin extraction separation and purification mainly contains solvent method (comprising water extraction, acid-hydrolysis method, alcohol extracting method, propyl carbinol method), lead salt method, column chromatography (alumina column chromatography, silica gel column chromatography, polyamide column chromatography, macroporous adsorbent resin) etc.Although adopted at present some more advanced technology as: effective ingredient in the microwave auxiliary extraction root of kudzu vine, adopt absorption with macroporous adsorbent resin puerarin to be equal to, separate the puerarin purity obtaining lower, do not reach medicinal object.Therefore be necessary that the puerarin crude product that separation is obtained carries out purifying and makes its content reach more than 97% (national standard), and reach medical grade standard.
Summary of the invention
The object of the present invention is to provide a kind of puerarin process for refining, be intended to solve the puerarin purity that the method for existing puerarin extraction separation and purification obtains lower, do not reach the problem of medicinal object.
The present invention is achieved in that a kind of puerarin process for refining comprises the following steps:
Step 1, methyl alcohol, ethanol, as the solvating agent of puerarin, using distilled water, glacial acetic acid as precipitation agent, using chloroform, anhydrous diethyl ether, distilled water and glacial acetic acid as cleaner, are removed water miscible, fat-soluble, acid soluble impurity.
Step 2, stationary phase using aluminum oxide, flyash during as column chromatography, remove the impurity such as pigment.
Step 3, use macroporous adsorbent resin carry out the refining of puerarin.
Further, the solvating agent described in step 1 is made up of 20% methyl alcohol and 80% ethanol, and specific inductivity is 35 left and right.
Further, propose puerarin removing after pigment, after purity acquires a certain degree, just can adopt macroporous adsorbent resin to refine.
Effect gathers
Beneficial effect of the present invention is as follows:
(1) several different methods is organically combined to puerarin purification, make the purity of puerarin exceed 99%, yield is 42%～45%.
(2) exceed 27%～30% than the yield of Zhang Zhongjing modern Chinese herbal medicine company limited (12%～18%), the production per kilogram sterling puerarin only raw materials cost of use can reduce by 1500 yuan, the comprehensive costs such as water power, fuel, solvent can reduce by 100 yuan, this enterprise produces 3 tons of meters of sterling puerarin at present per year, this project can increase income 5,400,000 yuan after using every year.
(3) the effective constituent Radix Puerariae flavone in the root of kudzu vine (take puerarin as representative), there is reduction myocardial consumption of oxygen, increase the pharmacological actions such as coronary artery, brain vessel blood amount, allevating angina pectoris and anti-arrhythmia,, develop medicinal highly purified puerarin and will produce larger society and environmental benefit.
Accompanying drawing explanation
Fig. 1 is the puerarin process for refining schema that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Fig. 1 shows puerarin process for refining flow process of the present invention, and as shown in the figure, the present invention is achieved in that a kind of puerarin process for refining comprises the following steps:
S101: methyl alcohol, ethanol, as the solvating agent of puerarin, using distilled water, glacial acetic acid as precipitation agent, using chloroform, anhydrous diethyl ether, distilled water and glacial acetic acid as cleaner, are removed water miscible, fat-soluble, acid soluble impurity.
S102: the stationary phase using aluminum oxide, flyash during as column chromatography, remove the impurity such as pigment.
S103: use macroporous adsorbent resin to carry out the refining of puerarin.
Further, the solvating agent described in S101 is made up of 20% methyl alcohol and 80% ethanol, and specific inductivity is 35 left and right.
Further, propose puerarin removing after pigment, after purity acquires a certain degree, just can adopt macroporous adsorbent resin to refine.
The present invention just several different methods organically combines puerarin purification, makes the purity of puerarin exceed 99%, and yield, 42%～45%, can reduce the production cost of enterprise greatly, has good social benefit and application prospect.
By reference to the accompanying drawings the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various modifications that performing creative labour can make or distortion still within protection scope of the present invention.
Claims (3)
1. a puerarin process for refining, is characterized in that, described puerarin process for refining comprises the following steps:
Step 1, methyl alcohol, ethanol, as the solvating agent of puerarin, using distilled water, glacial acetic acid as precipitation agent, using chloroform, anhydrous diethyl ether, distilled water and glacial acetic acid as cleaner, are removed water miscible, fat-soluble, acid soluble impurity;
Step 2, stationary phase using aluminum oxide, flyash during as column chromatography, remove pigment impurity;
Step 3, use macroporous adsorbent resin carry out the refining of puerarin.
2. puerarin process for refining as claimed in claim 1, is characterized in that, in step 1, solvating agent is made up of 80% methyl alcohol and 80% ethanol, and specific inductivity is 35.
3. puerarin process for refining as claimed in claim 1, is characterized in that, proposes puerarin and is removing after pigment, after purity reaches, adopts macroporous adsorbent resin to refine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410117256.0A CN103864767A (en) | 2014-03-26 | 2014-03-26 | Puerarin refining process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410117256.0A CN103864767A (en) | 2014-03-26 | 2014-03-26 | Puerarin refining process |
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| CN103864767A true CN103864767A (en) | 2014-06-18 |
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| CN201410117256.0A Pending CN103864767A (en) | 2014-03-26 | 2014-03-26 | Puerarin refining process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184163A (en) * | 2019-07-12 | 2019-08-30 | 劲牌有限公司 | A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function |
| CN111393422A (en) * | 2020-05-07 | 2020-07-10 | 四川轻化工大学 | Method for producing puerarin from radix Puerariae |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100799468B1 (en) * | 2006-06-30 | 2008-01-30 | 경남대학교 산학협력단 | Method for extracting active components of Pueraria radix using far-infrared ray |
| CN102936242A (en) * | 2012-11-28 | 2013-02-20 | 云南省农业科学院药用植物研究所 | Method for comprehensively utilizing arrowroot |
| CN103224491A (en) * | 2013-05-22 | 2013-07-31 | 天津道谷生物科技有限公司 | Method for extracting high-purity puerarin by using water as solvent |
| CN103254185A (en) * | 2013-05-25 | 2013-08-21 | 湖北省三鑫生物科技有限公司 | Novel process for producing puerarin by utilizing root of kudzu vine |
-
2014
- 2014-03-26 CN CN201410117256.0A patent/CN103864767A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100799468B1 (en) * | 2006-06-30 | 2008-01-30 | 경남대학교 산학협력단 | Method for extracting active components of Pueraria radix using far-infrared ray |
| CN102936242A (en) * | 2012-11-28 | 2013-02-20 | 云南省农业科学院药用植物研究所 | Method for comprehensively utilizing arrowroot |
| CN103224491A (en) * | 2013-05-22 | 2013-07-31 | 天津道谷生物科技有限公司 | Method for extracting high-purity puerarin by using water as solvent |
| CN103254185A (en) * | 2013-05-25 | 2013-08-21 | 湖北省三鑫生物科技有限公司 | Novel process for producing puerarin by utilizing root of kudzu vine |
Non-Patent Citations (1)
| Title |
|---|
| 刘玉洁,等: "葛根素的分离纯化方法研究", 《武警医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110184163A (en) * | 2019-07-12 | 2019-08-30 | 劲牌有限公司 | A kind of preparation method with the kudzuvine root wine for reducing hepatic injury function |
| CN111393422A (en) * | 2020-05-07 | 2020-07-10 | 四川轻化工大学 | Method for producing puerarin from radix Puerariae |
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Application publication date: 20140618 |
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