CN103861124A - Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents - Google Patents
Preparation method of platinum nanoclusters and application of platinum nanoclusters in tumor imaging agents and apoptosis agents Download PDFInfo
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Abstract
The invention discloses a preparation method of platinum nanoclusters and application of the platinum nanoclusters in tumor imaging agents and apoptosis agents. The preparation method comprises the following specific steps of: mixing a platinum salt solution and ascorbic acid (VC) and reduced glutathione (GSH), and heating in a water bath, wherein the synthesis speed and the particle size of the platinum nanoclusters are controlled by adjusting the pH and temperature of a solution and a ratio of components. The prepared platinum nanoclusters have a small particle size, good permeability, and excellent biocompatibility and stable fluorescence characteristics, can be used for high spatial and temporal resolution imaging on tumors and other lesion sites, and can effectively promote tumor cell apoptosis and tumor tissue ablation; the method and the application are simple and easy to implement, the toxicity is low, the sensitivity is high, the multi-mode real-time dynamic monitoring can be carried out, and the medical clinical application value and prospect are broad.
Description
Technical field
The present invention relates to a kind of preparation method of platinum cluster and the application in preparation and the apoptosis agent of tumor.The relevant platinum cluster preparation that this invention utilization makes is as the probe pathological changes sites such as tumor of precisely leading, and can, further combined with the photo-thermal therapy by Imaging Guidance, realize highly sensitive diagnosis and treatment to tumor.
Background technology
At present, the death of malignant tumor (cancer) still occupies first of all kinds of causes of the death in the world, becomes a large killer who captures now people's life.Because cancer early symptom is not obvious, be difficult to be found early, thereby the early diagnosis and therapy of cancer is still one of most important medical problem urgently to be resolved hurrily, very crucial to the healing of cancer.
In recent years, be accompanied by the development of Nanometer scale science and technology, some special natures that nano material possesses because of himself get most of the attention in the research for the treatment of of cancer.Nano material is because its particle diameter is little, easily pass through barrier cell, and the enhanced permeability and retention effect hyperfunction due to tumor tissues microvascular permeability and unsound lymphatic drainage system produces, make it be easy to preferentially assemble at tumor locus, in addition metal nano material can absorbing light and the raw free radical of delivery in hot weather or temperature raise, thereby be extremely conducive to the effect such as imaging and photo-thermal treatment of tumor.But in photo-thermal treatment process, the biocompatibility of nano material is a maximum challenge all the time, platinum cluster has good biocompatibility, uses platinum cluster to carry out the imaging of tumor and by reports such as the photo-thermal therapies of Imaging Guidance but up to the present have not yet to see.
Summary of the invention
For the defect of currently available technology, the invention provides preparation method and the application thereof of a kind of quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch.Glutathion is wrapped in platinum cluster surface and has greatly reduced cytotoxicity, under ultraviolet lighting, can carry out fast imaging detection to tumor locus, can kill again tumor cell under infrared light heat effect.
Technical scheme is:
The preparation method of platinum cluster, comprises the steps: by reductive glutathione (GSH) aqueous solution and ascorbic acid (vitamin C, VC) aqueous solution, then adds platinum saline solution, mix homogeneously, reacts and get final product.
Preferably, described platinum salt is the mixing of one or any two kinds in chloroplatinic acid, potassium chloroplatinate, potassium chloroplatinite, ammonium chloroplatinite, ammonium chloroplatinate, potassium chloroplatinite, platinum nitrate, dichloro four ammino platinum, dinitroso diammonia platinum.
Preferably, the mol ratio between reductive glutathione, ascorbic acid and platinum salt is (1～2): (1～2): 1.
Preferably, the concentration of reductive glutathione aqueous solution is 0.01～0.1 mol/L;
Preferably, the concentration of aqueous ascorbic acid is 0.01～0.1mol/L;
Preferably, platinum saline solution concentration is 0.01～0.1mol/L;
Preferably, become when orange and stop when reaction proceeds to solution;
Preferably, reaction temperature is 5～80 ℃.
Another aspect, the invention provides the application of above-mentioned platinum cluster in the preparation of tumor cell;
Another aspect, the invention provides the application of above-mentioned platinum cluster in the apoptosis agent of tumor cell.
Described tumor cell can be the tumor cell lines such as hepatocarcinoma, pulmonary carcinoma, cervical cancer, leukemia, osteosarcoma.
beneficial effect
1. the present invention utilizes the synergism of ascorbic acid (vitamin C) and reductive glutathione, and in conjunction with the corrasion of sulfydryl on glutathion, set up the preparation method of quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch, wherein ascorbic acid add the reduction that is conducive to reductive glutathione.
2. the glutathion that the present invention uses is required composition in target cell, and platinum cluster has wrapped up this kind of glutathion can greatly be reduced cytotoxicity and strengthen its targeting.
3. the present invention has realized the accurate fast imaging of tumor based on platinum cluster and the photo-thermal therapy by Imaging Guidance, for the disease early discoverys such as cancer and treatment provide new method and foundation.
4. this invention can realize not damaged, original position, Real-time and Dynamic neoplasm targeted therapy, further combined with fluorescence, Raman, ultrasonic, CT and nuclear-magnetism etc., can carry out polymorphic with multi-modal diagnosis and accurately the targeting location and treatment of synchronizeing, there is wide medical application prospect.
5. the present invention finds and has set up the new method of quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch, and apply to the fast imaging of tumor and the photo-thermal therapy by Imaging Guidance, for the disease early discoverys such as cancer and treatment provide new method and foundation.
Accompanying drawing explanation
Fig. 1 is the synthetic fluorescence spectrum figure afterwards of platinum cluster in embodiment 1;
Fig. 2 is that the ascorbic acid of different amounts in embodiment 1 reference examples is on the synthetic impact of platinum cluster.
Fig. 3 a-Fig. 3 c is embodiment 7 fluorescence tomoscan photos, comprises matched group and experimental group, and wherein, Fig. 3 a is the photo that does not add the hepatoma carcinoma cell of platinum cluster; Fig. 3 b is the photo that adds the hepatoma carcinoma cell of platinum cluster; Fig. 3 c is the photo of the normal cell (L02) that adds platinum cluster.
The specific embodiment
The reductive glutathione of this experiment, ascorbic acid, the reagent such as chloroplatinic acid, potassium chloroplatinate, potassium chloroplatinite, ammonium chloroplatinite, ammonium chloroplatinate, potassium chloroplatinite are purchased from Chemical Reagent Co., Ltd., Sinopharm Group.This tests tumor cell used is the tumor cell lines such as hepatocarcinoma, pulmonary carcinoma, cervical cancer, leukemia, osteosarcoma.
the preparation method of 1 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting chloroplatinic acid in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.05mol/L of 0.05 mol/L, adding concentration is the platinum reagent water solution of 0.05mol/L again, the volume ratio of three kinds of solution is 1:1:1, fully mix, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.2～2.0nm of good stability uniform particle diameter.
The fluorescence spectrum figure of the platinum fluorescence nano that the present embodiment prepares bunch as shown in Figure 1, in figure, curve 1 is the fluorescent spectrum curve of platinum cluster, curve 2 is fluorescent spectrum curves of the mixed solution of reductive glutathione (GSH) and ascorbic acid, the 3rd, the fluorescent spectrum curve of platinum acid chloride solution, as can be seen from the figure made platinum cluster emission wavelength is in 456nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
reference examples changes the building-up process of ascorbic acid content in component
Be with the difference of embodiment 1: in synthetic process, do not add or add the ascorbic acid solution of different content, specifically as follows:
1) reductive glutathione, ascorbic acid and the platinum reagent water solution of preparation different proportion.
2) reductive glutathione and the aqueous ascorbic acid that in the chloroplatinic acid aqueous solution that is 0.05mol/L in concentration, add respectively variable concentrations, the volume ratio of three kinds of aqueous solutions is 1:1:1, fully mixes, then 37 ℃ of heating in water bath are placed; Carry out altogether 5 kinds of variable concentrations ratios batch, in each batch of corresponding diagram 2, be numbered 1～5 curve, concentration is as shown in the table:
The ascorbic acid (numeral in Fig. 2 in the legend of each curve is the relative amount of representative ascorbic acid in glutathion and ascorbic acid mole summation) of the amount mark of the different material after having reacted on the synthetic impact figure of platinum cluster as shown in Figure 2, does not as can be seen from the figure have glutathion or ascorbic acid all can not obtain platinum fluorescence nano bunch.Contrast with embodiment 1, can find out, ascorbic acid has played good reduction process synergism, and reaction is normally carried out.The corresponding proportioning raw materials optimum of curve 3, obtains the fluorescence intensity maximum of product.
the preparation method of 2 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting potassium chloroplatinite in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.01mol/L of 0.1 mol/L, adding concentration is the platinum reagent water solution of 0.05mol/L again, the volume ratio of three kinds of solution is 2:2:1, three fully mixes, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.4～2.2nm of good stability uniform particle diameter.
The emission wavelength of the platinum fluorescence nano that the present embodiment prepares bunch is in 455nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
the preparation method of 3 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting ammonium chloroplatinite in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.1mol/L of 0.01 mol/L, adding concentration is the platinum reagent water solution of 0.05mol/L again, the volume ratio of three kinds of solution is 2:1:1, three fully mixes, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.4～2.3nm of good stability uniform particle diameter.
The emission wavelength of the platinum fluorescence nano that the present embodiment prepares bunch is in 457nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
the preparation method of 4 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting ammonium chloroplatinate in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.05mol/L of 0.05 mol/L, adding concentration is the platinum reagent water solution of 0.01mol/L again, the volume ratio of three kinds of solution is 1:2:1, three fully mixes, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.3～2.1nm of good stability uniform particle diameter.
The emission wavelength of the platinum fluorescence nano that the present embodiment prepares bunch is in 456nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
the preparation method of 5 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting chloroplatinic acid in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.05mol/L of 0.05 mol/L, adding concentration is the platinum reagent water solution of 0.1mol/L again, the volume ratio of three kinds of solution is 1:1:1, three fully mixes, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.1～2.4nm of good stability uniform particle diameter.
The emission wavelength of the platinum fluorescence nano that the present embodiment prepares bunch is in 457nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
the preparation method of 6 one kinds of the embodiment quick one-step synthesis platinum fluorescence nano based on reductive glutathione bunch:
1) preparation reductive glutathione (GSH) and platinum saline solution (adopting chloroplatinic acid in the present embodiment).
2) by concentration be ascorbic acid (vitamin C) aqueous solution of reductive glutathione aqueous solution and the 0.05mol/L of 0.05 mol/L, adding concentration is the platinum reagent water solution of 0.05mol/L again, three is fully mixed, then 37 ℃ of heating of water-bath, become when orange and have reacted until solution.
The platinum fluorescence nano preparing in the present embodiment bunch about 1.5～2.5nm of good stability uniform particle diameter.
The emission wavelength of the platinum fluorescence nano that the present embodiment prepares bunch is in 457nm left and right, with the Pt of bibliographical information
0absorbing wavelength consistent.
the accurate fast imaging of the tumor of embodiment 7 based on platinum cluster
1) after platinum cluster high speed centrifugation embodiment 1 being prepared, sterilizing, is arrived in PBS (pH=7.2) and is prepared against and use by ultrasonic dissolution, and concentration is 100 μ g/ml.
2) using hepatoma carcinoma cell (HepG2) target cell as object of study, experimental group by the hepatoma carcinoma cell in exponential phase (HepG2) according to 1.6 × 10
5the density of individual cells/well is inoculated in 6 orifice plates, cultivates the platinum cluster PBS solution that adds step 1) gained after 24 h, hatch altogether 12 ~ 72 hours (37 ℃, 5 % CO
2, RH 95%), as experimental group.
3) matched group by the hepatoma carcinoma cell in culture medium (HepG2) target cell according to 1.6 × 10
5the density of individual cells/well is inoculated in 6 orifice plates, cultivates 24 h.
4) every hole that incubation time stops backward experimental group and matched group adds phosphate buffer solution (PBS, pH=7.2) to rinse 2-3 time.
5) with confocal fluorescent microscope etc., platinum cluster is carried out to qualitative and quantitative analysis.Cell is placed under laser focusing fluorescence microscope, adopting wavelength is that 405 nm ultraviolet lights excite the blue-fluorescence image that can collect cell, can observe clearly this platinum nano-cluster mainly concentrate on tumor cell by fluorescence layer scanning technology.Scanned picture as shown in Fig. 3 a-Fig. 3 c, as can be seen from the figure, experimental group (Fig. 3 b) can demonstrate tumor cell significantly, and matched group (Fig. 3 fails to demonstrate cellularity in a); In Fig. 3 c, normal cell and platinum cluster binding ability are bad, can not demonstrate image clearly simultaneously.
application in the photo-thermal therapy of the accurate fast imaging guidance of the tumor of embodiment 8 based on platinum cluster
The platinum cluster that the present invention makes can be in organism some pathological changes site of targeting, as tumor cell/tissue, realize high time-space resolution fluorescent labeling to lesions positions such as tumors and by the multi-modal photo-thermal therapy of Imaging Guidance, effectively promote apoptosis of tumor cells and tumor tissues to melt.Relevant platinum cluster is as follows in the imaging of tumor cell and the concrete steps of photo-thermal therapy application:
1), after the platinum cluster high speed centrifugation that embodiment 1 is prepared, sterilizing, is arrived in PBS (pH=7.2) and is prepared against and use by ultrasonic dissolution, and concentration is 100 μ g/ml.
2), as experimental group, select hepatoma carcinoma cell (HepG2) target cell as object of study, experimental group by the hepatoma carcinoma cell in exponential phase (HepG2) according to 1.6 × 10
5the density of individual cells/well is inoculated in 6 orifice plates, adds the platinum cluster solution of step 1 gained after cultivation 24 h, jointly hatches after 8 ~ 12 hours, uses near infrared light 10 ~ 30 minutes, and then continues to cultivate 12 ~ 24 hours.
3), as a control group 1, by the hepatoma carcinoma cell in culture medium (HepG2) according to 1.6 × 10
5the density of individual cells/well is inoculated in 6 orifice plates, and experimental group carries out identical incubation time section, with near infrared light 10 ~ 30 minutes, and then continues to cultivate 12 ~ 24 hours.
4), as a control group 2, by the hepatoma carcinoma cell in culture medium (HepG2) according to 1.6 × 10
5the density of individual cells/well is inoculated in 6 orifice plates, adds the platinum cluster solution of step 1 gained after cultivation 24 h, jointly hatches after 8 ~ 12 hours, different from experimental group: do not carry out Infrared irradiation, and then continue to cultivate 12 ~ 24 hours.
5) every hole that incubation time stops backward experimental group and matched group adds phosphate buffer solution (PBS, pH=7.2) rinse 2-3 time, with trypsinization, Trypan Blue, finally use confocal fluorescent microscope observing cell state, fluorescence distribution situation and its fluorescence intensity by fluorescence imaging are carried out qualitative or quantitative analysis to platinum cluster component, and dyeing counting apoptosis amount.
Data result shows: use Infrared irradiation to add the apoptosis of tumor cells of this platinum cluster obvious, and do not add illumination or add irradiation under ultraviolet ray, cell is not almost affected.
The above is only the preferred embodiment of the present invention; be noted that for those skilled in the art; under the premise without departing from the principles of the invention, can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a preparation method for platinum cluster, is characterized in that, comprises the steps: reductive glutathione aqueous solution to mix with aqueous ascorbic acid, then adds platinum saline solution, and mix homogeneously, reacts and get final product.
2. the preparation method of platinum cluster according to claim 1, is characterized in that: described platinum salt is the mixture of one or any two kinds in chloroplatinic acid, potassium chloroplatinate, potassium chloroplatinite, ammonium chloroplatinite, ammonium chloroplatinate, potassium chloroplatinite, platinum nitrate, dichloro four ammino platinum, dinitroso diammonia platinum.
3. the preparation method of platinum cluster according to claim 1, is characterized in that: the concentration of reductive glutathione aqueous solution is 0.01～0.1 mol/L.
4. the preparation method of platinum cluster according to claim 1, is characterized in that: the concentration of aqueous ascorbic acid is 0.01～0.1mol/L.
5. the preparation method of platinum cluster according to claim 1, is characterized in that: platinum saline solution concentration is 0.01～0.1mol/L.
6. the preparation method of platinum cluster according to claim 1, is characterized in that: reaction temperature is 5～80 ℃.
7. the application of the platinum cluster described in claim 1～6 any one in the preparation of tumor cell.
8. the application of platinum cluster according to claim 7 in the preparation of tumor cell, is characterized in that: described tumor cell refers to hepatocarcinoma, pulmonary carcinoma, cervical cancer, leukemia, osteosarcoma cell line.
9. the application of the platinum cluster described in claim 1～6 any one in the apoptosis agent of tumor cell.
10. the application of platinum cluster according to claim 9 in the apoptosis agent of tumor cell, is characterized in that: described tumor cell refers to hepatocarcinoma, pulmonary carcinoma, cervical cancer, leukemia, osteosarcoma cell line.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106619568A (en) * | 2016-12-09 | 2017-05-10 | 浙江大学 | Preparation method of platinum nanocrystal composite nanomaterial, and product of preparation method |
| CN108042800A (en) * | 2017-11-16 | 2018-05-18 | 华中科技大学 | Bivalent state platinum cluster of temperature sensitive polymer modification and its preparation method and application |
| CN108841385A (en) * | 2018-04-27 | 2018-11-20 | 中原工学院 | A kind of preparation method and application of the fluorescence platinum nanoclusters of hematological system suspension cell optical imagery |
| CN108855240A (en) * | 2018-06-25 | 2018-11-23 | 厦门大学 | A method of nano-platinum particle catalytic activity is protected using glycerol |
| CN110125434A (en) * | 2019-05-14 | 2019-08-16 | 东南大学 | A kind of preparation method of photo-thermal gold nano-material |
| CN110237254A (en) * | 2019-06-05 | 2019-09-17 | 大连工业大学 | A kind of preparation method and applications of the food-borne anti-oxidation peptide optothermal material of multi-metal oxygen cluster- |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2226082A2 (en) * | 2009-03-05 | 2010-09-08 | Universität Duisburg-Essen | Control of the toxicity of gold nanoparticles |
| CN103083687A (en) * | 2013-01-16 | 2013-05-08 | 东南大学 | Application of silver and platinum nano-cluster in tumor targeted imaging |
-
2014
- 2014-03-03 CN CN201410075034.7A patent/CN103861124B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2226082A2 (en) * | 2009-03-05 | 2010-09-08 | Universität Duisburg-Essen | Control of the toxicity of gold nanoparticles |
| CN103083687A (en) * | 2013-01-16 | 2013-05-08 | 东南大学 | Application of silver and platinum nano-cluster in tumor targeted imaging |
Non-Patent Citations (2)
| Title |
|---|
| M. MANIKANDAN ET AL.: "Platinum nanoparticles for the photothermal treatment of Neuro 2A cancer cells", 《BIOMATERIALS》, vol. 34, 30 April 2013 (2013-04-30), XP028541472, DOI: doi:10.1016/j.biomaterials.2013.03.077 * |
| XAVIER LE GUÉVEL ET AL.: "Synthesis of Yellow-Emitting Platinum Nanoclusters by Ligand Etching", 《THE JOURNAL OF PHYSICAL CHEMISTRY》, vol. 116, 21 February 2012 (2012-02-21) * |
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| CN106619568A (en) * | 2016-12-09 | 2017-05-10 | 浙江大学 | Preparation method of platinum nanocrystal composite nanomaterial, and product of preparation method |
| CN106619568B (en) * | 2016-12-09 | 2020-01-17 | 浙江大学 | Preparation method of platinum nanocrystalline composite nanomaterial and product |
| CN108042800A (en) * | 2017-11-16 | 2018-05-18 | 华中科技大学 | Bivalent state platinum cluster of temperature sensitive polymer modification and its preparation method and application |
| CN108042800B (en) * | 2017-11-16 | 2020-05-26 | 华中科技大学 | Temperature-sensitive polymer modified bivalent platinum nano-cluster and preparation method and application thereof |
| CN108841385A (en) * | 2018-04-27 | 2018-11-20 | 中原工学院 | A kind of preparation method and application of the fluorescence platinum nanoclusters of hematological system suspension cell optical imagery |
| CN108841385B (en) * | 2018-04-27 | 2020-12-25 | 中原工学院 | Preparation method and application of fluorescent platinum nanocluster for optical imaging of blood system suspension cells |
| CN108855240A (en) * | 2018-06-25 | 2018-11-23 | 厦门大学 | A method of nano-platinum particle catalytic activity is protected using glycerol |
| CN110125434A (en) * | 2019-05-14 | 2019-08-16 | 东南大学 | A kind of preparation method of photo-thermal gold nano-material |
| CN110237254A (en) * | 2019-06-05 | 2019-09-17 | 大连工业大学 | A kind of preparation method and applications of the food-borne anti-oxidation peptide optothermal material of multi-metal oxygen cluster- |
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