CN103859141B - A kind of physical modification preparation method of high dissolubility rice protein - Google Patents
A kind of physical modification preparation method of high dissolubility rice protein Download PDFInfo
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- CN103859141B CN103859141B CN201410109957.XA CN201410109957A CN103859141B CN 103859141 B CN103859141 B CN 103859141B CN 201410109957 A CN201410109957 A CN 201410109957A CN 103859141 B CN103859141 B CN 103859141B
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Abstract
The present invention discloses a kind of physical modification preparation method of high dissolubility rice protein.Raw Materials Rice albumen is scattered in water by the method, through base extraction, freezing, pulverizes, and after acid neutralization, centrifuging and taking supernatant, spraying dry, obtains high dissolubility rice protein finished product.The rice protein product with high dissolubility can be prepared by the inventive method, its protein solubility >=98%, emulsibility >=0.50, foaming characteristic >=17.39, substantially increase utilization rate and the added value of rice protein, be with a wide range of applications.
Description
Technical field
The invention belongs to rice protein finishing technology field, be specifically related to the soluble rice protein that a kind of physical modification preparation method of high dissolubility rice protein and the method prepare.
Background technology
Rice is China's staple food grain.The protein efficiency value (PER) of rice protein is between 2.0 and 2.5, digestibility is more than 90%, meanwhile, the characteristic such as the reduced immunogenicity of rice protein, antitumor, anti-hypertension, anti-angiogenic atherosis and anti-cholesterol has been proved, has very wide application prospect.But rice protein is poorly soluble is the main cause of its non-broad commercial applications always.Dissolubility is the important indicator of the processing of measurement product protein and application performance, decides the critical function such as emulsibility and foaming characteristic characteristic.
The current deliquescent method of rice protein of improving mainly contains chemical method and enzyme process: chemical method is structure, electrostatic charge and hydrophobic group by changing protein, thus improve its dissolution properties, but the external source group that chemical modification is introduced changes original composition of rice protein, causes nutrient protein to lose; Enzyme process utilizes protease to carry out catalyzing hydrolysis to protein molecular under certain condition, protein macromolecule is made to be degraded into the Small molecular of different chain length, protein is after enzyme hydrolysis, and except its functional character obviously changes, the peptide class that enzymolysis generates more easily is absorbed by the body digestion, but hydrolysis degree is wayward, and cost is relatively high, if hydrolysis degree is less, performance improvement is not obvious, if hydrolysis degree is comparatively large, the peptide class of generation makes product with bitter taste.Physical method is also used to the solubilising modification of protein, such as ultrasonic, the processing method such as super-pressure, jet cooking method, microjet method, but these methods are quite limited for the improvement effect of rice protein solubility, are not suitable for suitability for industrialized production.
Summary of the invention
For the above-mentioned defect that the existing rice protein method that has improved solubility exists, the applicant is through Improvement, a kind of physical modification preparation method of high dissolubility rice protein is provided, rice protein dissolubility >=98% prepared by this method, emulsibility >=0.50, foaming characteristic >=17.39, substantially increase utilization rate and the added value of rice protein.
Technical scheme of the present invention is as follows:
A physical modification preparation method for high dissolubility rice protein, is scattered in water by Raw Materials Rice albumen, through base extraction, freezing, pulverizes, and acid neutralization, centrifuging and taking supernatant, spraying dry, obtains high dissolubility rice protein finished product.
Preferably, the inventive method comprises the following steps:
(1) dispersion and alkali treatment: be scattered in by rice protein in water, solid-liquid ratio 1:15 ~ 25, stir and evenly mix, and by NaOH solution, above-mentioned rice protein dispersion liquid pH value are adjusted to 10.5 ~ 12.5, is preferably pH12.5;
(2) freezing: step (1) gained feed liquid is carried out freezing in-20 ~-10 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until this solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 ~ 7.5 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 10 ~ 20min of 1000g, collect supernatant;
(6) repeat: by (5) gained sediment by step (1) ~ (5) reprocessing 2 ~ 3 times;
(7) dry: to carry out spraying dry after step (5) and step (6) gained supernatant being merged, obtain high dissolubility rice protein finished product.Particularly, step (7) described spray drying control system spray dryer inlet temperature 110 ~ 140 DEG C, outlet temperature 80 ~ 100 DEG C.
The high dissolubility rice protein prepared by the inventive method is with a wide range of applications, and can apply to food, medicine, cosmetics and high-end health products trade.
For the rice protein adopting the inventive method to prepare, adopt Kjeldahl's method method to measure protein recovery and dissolubility, adopt turbidity method to measure emulsibility, adopt expansion rate method to measure foaming characteristic, concrete assay method is as follows:
(1) product protein recovery
According to the present invention, the protein recovery albumen quality be construed as in soluble rice protein product accounts for the percentage of albumen quality in Raw Materials Rice albumen.
With the albumen quality m in soluble rice protein product after Kjeldahl nitrogen determination spraying dry and the albumen quality m0 in material protein.
The protein recovery of soluble rice protein represents with following formula:
Protein recovery (%)=(m/m
0) × 100
In formula: m---the albumen quality after spraying dry in soluble rice protein product;
M
0---albumen quality in material protein.
(2) dissolubility
According to the present invention, dissolubility is construed as in the soluble rice protein product of per unit mass, and soluble albumen quality accounts for the percentage of albumen quality in product.
Soluble rice protein sample 50mmol/L potassium dihydrogen phosphate-sodium hydrate buffer solution (pH7.0) is made into 1%(W/V) solution, 1h is stirred at 25 DEG C, the centrifugal 10min of 3000r/min, then measure the albumen quality m1 in supernatant and the albumen quality m in soluble rice protein product respectively with Kjeldahl's method.
Soluble rice protein solubility following formula represents:
Solubility (%)=(m of protein
1/ m) × 100
In formula: m
1---albumen quality in supernatant;
M---the albumen quality in soluble rice protein product.
(3) emulsibility
According to the present invention, emulsibility is construed as the character that protein can form emulsion.
Get the protein solution 30ml of 1% concentration, add 10ml soybean salad oil, 10000r/min stirs 1min; 0.1ml emulsion is got from bottom, rapidly with 5ml0.1%(w/v with syringe) lauryl sodium sulfate (SDS) solution mixes, colorimetric under 500nm wavelength, record absorbance A 0.
The emulsifying capacity of protein represents with following formula:
EA=A
0
In formula: EA---emulsibility;
A
0---the absorbance after emulsion dilution.
(4) foaming characteristic
According to the present invention, foaming characteristic is construed as the character of the formation helping dispersion gas phase.
Rice protein sample 50mmol/L potassium dihydrogen phosphate-NaOH (pH7.0) buffer solution is made into 0.3% strength solution, gets 100ml and pour in high-speed tissue mashing machine, 10000r/min stirs 1min; Proceed to 250ml graduated cylinder, record foam volume V as early as possible
0.
Protein foamability calculates according to following formula:
FC=V
0
In formula: FC---foaming characteristic;
V
0---foam volume.
The present invention has following Advantageous Effects:
The present invention's whole process adopts physical method (dispersion and alkali treatment, freezing-to pulverize, acid neutralizes, centrifugal, spraying dry etc.) to carry out modification to rice protein, first, dispersion and alkali treatment suitably can reduce the aggregation extent of rice protein, increase the action site of protein molecule and hydrone; Under alkali condition (pH10.5 ~ 12.5) freezing-pulverization process can change aggregation extent and the protein conformation of rice protein, protein aggregation degree reduces, and protein conformation launches, and the hydrophilic radical of albumen is exposed, reaches a kind of effect of dissolving rice protein.Rice protein product (protein solubility >=98% with high dissolubility can be prepared by various processes synergy of the present invention, emulsibility >=0.50, foaming characteristic >=17.39), compared with Raw Materials Rice albumen, rice protein solubility, emulsibility and foaming characteristic best result can not improve 79 times, 132% and 112%, substantially increase utilization rate and the added value of rice protein; Especially, when alkali treatment pH value is 12.5, protein recovery can reach 50.43%.Present invention, avoiding in chemical modification or enzyme modification process and introduce foreign gene or change protein composition, modified effect is remarkable, made high-quality, high dissolubility rice protein can be applicable to food, medicine, cosmetics and high-end health products trade, are with a wide range of applications.
Accompanying drawing explanation
Fig. 1 is present invention process flow chart.
Detailed description of the invention
Below in conjunction with accompanying drawing, and by embodiment, the present invention is specifically described.
Preparation embodiment
Embodiment 1
The present embodiment method step is as follows:
(1) dispersion and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 15L water, stirs and evenly mixs, by NaOH solution, gained rice protein dispersion liquid pH value is adjusted to 10.5;
(2) freezing: step (1) gained feed liquid is carried out freezing processing in-10 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 10min of 1000g, collect supernatant;
(6) repeat: by step (5) gained sediment by step (1) ~ (5) reprocessing 2 times;
(7) dry: carry out spraying dry after step (5) and step (6) gained supernatant being merged, the inlet temperature controlling spray dryer in spray-drying process is 110 DEG C, and outlet temperature is 80 DEG C, obtains high dissolubility rice protein finished product 53.7g.
Embodiment 2
The present embodiment method step is as follows:
(1) dispersion and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 20L water, stirs and evenly mixs, by NaOH solution, gained rice protein dispersion liquid pH value is adjusted to 11.5;
(2) freezing: step (1) gained feed liquid is carried out freezing processing in-15 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 15min of 1000g, collect supernatant;
(6) repeat: by step (5) gained sediment by step (1) ~ (5) reprocessing 2 times;
(7) dry: carry out spraying dry after step (5) and step (6) gained supernatant being merged, the inlet temperature controlling spray dryer in spray-drying process is 125 DEG C, and outlet temperature is 90 DEG C, obtains high dissolubility rice protein finished product 140.6g.
Embodiment 3
The present embodiment method step is as follows:
(1) dispersion and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 20L water, stirs and evenly mixs, by NaOH solution, gained rice protein dispersion liquid pH value is adjusted to 11.5;
(2) freezing: step (1) gained feed liquid is carried out freezing processing in-15 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 15min of 1000g, collect supernatant;
(6) repeat: by step (5) gained sediment by step (1) ~ (5) reprocessing 2 times;
(7) dry: carry out spraying dry after step (5) and step (6) gained supernatant being merged, the inlet temperature controlling spray dryer in spray-drying process is 125 DEG C, and outlet temperature is 90 DEG C, obtains high dissolubility rice protein finished product 140.6g.
Embodiment 3
The present embodiment method step is as follows:
(1) dispersion and alkali treatment: 1000g Raw Materials Rice albumen is scattered in 25L water, stirs and evenly mixs, by NaOH solution, gained rice protein dispersion liquid pH value is adjusted to 12.5;
(2) freezing: step (1) gained feed liquid is carried out freezing processing in-20 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 20min of 1000g, collect supernatant;
(6) repeat: by step (5) gained sediment by step (1) ~ (5) reprocessing 2 times;
(7) dry: carry out spraying dry after step (5) and step (6) gained supernatant being merged, the inlet temperature controlling spray dryer in spray-drying process is 140 DEG C, and outlet temperature is 100 DEG C, obtains high dissolubility rice protein finished product 504.3g.
Rice protein end properties measures
Measure the dissolubility of the made rice protein finished product of embodiment 1 ~ embodiment 3, emulsibility and emulsibility and analyze as stated above, result is as shown in table 1.
Table 1 high dissolubility rice protein performance measurement result
Can be obtained by table 1 test data analysis, the rice protein product prepared by physical modified method described in embodiment 1 ~ embodiment 3 has high dissolubility, protein solubility >=98%, emulsibility >=0.50, foaming characteristic >=17.39, compared with Raw Materials Rice albumen, rice protein solubility, emulsibility and foaming characteristic best result can not improve 79 times, 132% and 112%, especially, its protein recovery of rice protein product prepared by method described in embodiment 3 reaches 50.43%, is optimum embodiment.
Involved by above embodiment and Application Example, raw material and reagent are commercially available prod, and institute's use industrial equipment is this area conventional equipment.
The above is only the preferred embodiment of the present invention, the invention is not restricted to above embodiment.Be appreciated that the oher improvements and changes that those skilled in the art directly derive without departing from the spirit and concept in the present invention or associate, all should think and be included within protection scope of the present invention.
Claims (3)
1. a physical modification preparation method for high dissolubility rice protein, is characterized in that: be scattered in water by Raw Materials Rice albumen, through base extraction, freezing, pulverizes, after acid neutralization, and centrifuging and taking supernatant, spraying dry, obtains high dissolubility rice protein finished product;
Specifically comprise the following steps:
(1) dispersion and alkali treatment: be scattered in by rice protein in water, solid-liquid ratio 1:15 ~ 25, stir and evenly mix, and by NaOH solution, above-mentioned rice protein dispersion liquid pH value are adjusted to 10.5 ~ 12.5;
(2) freezing: step (1) gained feed liquid is carried out freezing in-20 ~-10 DEG C, until feed liquid fully charge;
(3) pulverize: freezing for step (2) gained solid feed liquid pulverizer is pulverized, until this solid becomes liquid completely;
(4) acid neutralization: step (3) gained material liquid pH value is adjusted to 7.0 ~ 7.5 with HCl solution;
(5) centrifugal: by step (4) gained feed liquid in the centrifugal 10 ~ 20min of 1000g, collect supernatant;
(6) repeat: by (5) gained sediment by step (1) ~ (5) reprocessing 2 ~ 3 times;
(7) dry: to carry out spraying dry after step (5) and step (6) gained supernatant being merged, obtain high dissolubility rice protein finished product.
2. the physical modification preparation method of high dissolubility rice protein as claimed in claim 1, is characterized in that: in step (1) described alkali treatment, described rice protein dispersion liquid pH value is adjusted to 12.5.
3. the physical modification preparation method of high dissolubility rice protein as claimed in claim 1, is characterized in that: step (7) described spray drying control system spray dryer inlet temperature 110 ~ 140 DEG C, outlet temperature 80 ~ 100 DEG C.
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| CN105660986A (en) * | 2016-01-19 | 2016-06-15 | 福建农林大学 | Method for improving dissolvability of rice protein isolates by supersonic wave-assisted alkali treatment |
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| CN107568410B (en) * | 2017-10-12 | 2020-12-01 | 江南大学 | Method for preparing rice protein with high-solubility common-frame structure |
| CN109892471B (en) * | 2017-12-08 | 2022-04-15 | 中粮集团有限公司 | Rice protein processing method and product |
| CN109757602B (en) * | 2019-01-29 | 2020-12-01 | 惠州华大辐照科技有限公司 | Method for improving rice protein solubility by combining alkaline method and electron beam irradiation |
| CN110200124B (en) * | 2019-07-02 | 2021-01-29 | 江南大学 | Preparation method of high-solubility rice protein |
| CN110810617A (en) * | 2019-11-06 | 2020-02-21 | 江西省科学院应用化学研究所 | Preparation method of rice gluten with high emulsifying activity and high emulsifying stability |
| CN112790270B (en) * | 2020-12-31 | 2023-04-11 | 江西金农米业集团有限公司 | Preparation method of soluble rice protein |
| CN113508863B (en) * | 2021-07-14 | 2023-04-14 | 江南大学 | Full-soluble edible fungus protein and preparation method thereof |
| CN114933630A (en) * | 2022-05-20 | 2022-08-23 | 华中农业大学 | Rice protein modification method and application |
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| CN102165989A (en) * | 2011-04-12 | 2011-08-31 | 江南大学 | Preparation method of soluble rice protein |
| CN103283934A (en) * | 2013-06-17 | 2013-09-11 | 安徽农业大学 | Preparation method of high-purity rice protein |
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| CN102165989A (en) * | 2011-04-12 | 2011-08-31 | 江南大学 | Preparation method of soluble rice protein |
| CN103283934A (en) * | 2013-06-17 | 2013-09-11 | 安徽农业大学 | Preparation method of high-purity rice protein |
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