CN103852456A - Equipment and method for quantitatively and rapidly detecting lead at constant temperature in real time - Google Patents
Equipment and method for quantitatively and rapidly detecting lead at constant temperature in real time Download PDFInfo
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- CN103852456A CN103852456A CN201310291702.5A CN201310291702A CN103852456A CN 103852456 A CN103852456 A CN 103852456A CN 201310291702 A CN201310291702 A CN 201310291702A CN 103852456 A CN103852456 A CN 103852456A
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Abstract
The invention relates to equipment for quantitatively and rapidly detecting lead at constant temperature in real time. The equipment comprises a timing temperature controller (1), a lead detection kit (2) and a heavy metal lead detector (3) which are sequentially connected with one another. After a sample to be detected is cultivated in the timing temperature controller, the sample is mixed and reacted with a detection reagent to generate signal change, the heavy metal lead detector monitors the signal change at the constant temperature and transmits the detected data to a built-in information processing device, and the information processing device analyzes the detected data and obtains the lead content of the sample. The invention also relates to a method for quantitatively and rapidly detecting lead at constant temperature in real time. The method comprises the following steps: starting detection equipment, and putting a quartz trace cuvette (7) into the detection equipment; putting the sample to be detected into a slot of the timing temperature controller (1), and cultivating at the constant temperature of 37 DEG C for 5 minutes; sucking a sample solution through a sample injector (6), connecting the tail end of the sample injector with a reagent reaction tube (5) in which a lead detection reagent (4) is arranged, injecting the sample into the quartz trace cuvette (7) through the reagent reaction tube (5), starting the test, and accurately displaying the detection result in real time. According to the equipment and the method, the problem that the heavy metal lead content cannot be rapidly detected in the field can be solved.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of field quick detection equipment that can be used for heavy metal lead.
Background technology
Lead is a kind of to all virose heavy metals of the many systems in human body, it is generally acknowledged that the comparatively safe standard of blood lead should not exceed 10-14 μ g/dl, but in recent years, along with the accelerated development of China's industrialization, urbanization process, lead contamination problem is also more and more serious.At present, lead contamination has become the merciless killer of human health, and the victim that lead contamination stands in the breach is children.Lead contamination has at present received people's concern, and national departments concerned has started to have taked corresponding measure.For security consideration, to content of heavy metal lead in underground water, surface water, industrial waste water, soil, gasoline, food carry out real-time on-site monitoring become in the urgent need to.But current existing technology, still can only depend on laboratory main equipment detects heavy metal lead as Atomic absorption, ICP-MS etc., device therefor is not only expensive, and cannot carry out fast detecting to the metallic lead in sample in actual field, thus real-time on-site monitoring lead content.
The present invention uses heavy metal enzymatic and alpha-non-natural amino acid fluorescence specific mark know-why, and a kind of checkout equipment and method that can in situ quantitation detects content of heavy metal lead is provided.
Summary of the invention
The object of the invention is the problem in order to solve field condition fast detecting content of heavy metal lead, and the apparatus and method for of a kind of real-time constant-temperature quantitative fast detecting heavy metal lead proposing.
The present invention by the following technical solutions, an equipment for real-time constant-temperature quantitative fast detecting lead, comprises the timing temp controller connecting successively, plumbous detection kit, heavy metal lead detector, after testing sample and detection reagent mix, react, produce signal intensity, heavy metal lead detector is monitored this signal intensity, and being sent to the built-in signal conditioning package of equipment by detecting data, this signal conditioning package, to detecting data analysis, obtains sample lead content;
Described plumbous detection kit comprises plumbous reagent, reagent reacting pipe, injector, the quartzy micro-cuvette of detecting;
Described lead detects pack containing a kind of specific substrate and a kind of fluorophor, and this fluorophor is connected on this specific substrate, forms fluorescence labeling specific substrate, and the lead in sample detects reagent at this lead and does the used time, and fluorophor is released, and causes signal intensity;
Described heavy metal lead detector comprises the light source connecting successively, the first monochromator, colorimetric pool, the second monochromator, detecting device;
Described monochromator is by entrance slit, quasi-optical device, dispersion element, and beam condensing unit, exit slit composition, quasi-optical device and beam condensing unit are lens, dispersion element is prism or grating;
Described heavy metal lead detector also comprises thermostat, for controlling temperature of reaction system, also comprises LED display, and for showing detection data, heavy metal lead detector also comprises USB interface.
Object of the present invention is also achieved through the following technical solutions, and a kind of method of real-time constant-temperature quantitative fast detecting lead, comprises the following steps:
S1, testing sample is placed in timing temp controller hole slot, 37 degrees Celsius of constant temperature are cultivated 5 minutes, and testing sample is carried out to constant temperature pre-service;
S2, start heavy metal lead detector, micro-quartz cuvette is put into checkout equipment, draw sample liquid with injector;
S3, injector end is connected with the built-in plumbous reagent reacting pipe that detects reagent, the sample reagent reacting pipe of flowing through, is injected in quartzy micro-cuvette;
S4, remove reagent reacting pipe and injector, cover the lid of sample test cup;
S5, press and start key and start test, sample detects reagent effect with plumbous, and signal intensity occurs, and in 2 minutes, shows testing result, record data, and detection completes;
Described lead detects pack containing a kind of specific substrate and a kind of fluorophor, this fluorophor is connected on this specific substrate, form fluorescence labeling specific substrate, the plumbous reagent that detects also comprises a kind of stabilizing agent, lead in sample detects reagent at this lead and does the used time, fluorophor is released, and causes signal intensity;
It is 0.5ml that described injector is drawn sample size in sample hose.
Technique scheme of the present invention has the following advantages compared to existing technology:
1. the present invention is by heavy metal enzyme technology principle, and design can be to the substrate of heavy metal specific recognition.
2. the present invention has adopted advanced fluorescence labeling technology, has guaranteed sensitivity, the accuracy of testing result.Very micro-when plumbous when having in sample, can there is catalytic reaction, equipment can detect the variation of fluorescence signal.
3. the data that the built-in signal conditioning package Real-time Collection of checkout equipment detects, the line number of going forward side by side is processed according to one's analysis, and the display screen that result can carry by equipment directly shows, and scene can obtain testing result accurately.In addition, equipment storing data automatically, and can at any time data be derived by USB interface.
4. the present invention uses a kind of built-in thermostat, can accurately control the temperature of reaction system and carry out under 37 degrees Celsius, to avoid the inconsistent impact on reaction system of temperature.
Accompanying drawing explanation
For content of the present invention is more likely to be clearly understood, below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is the device structure schematic diagram of real-time constant-temperature quantitative fast detecting lead;
Fig. 2 is plumbous detection kit structural representation;
Fig. 3 is heavy metal lead detector structural representation;
Fig. 4 is monochromator structural representation.
In figure, Reference numeral is expressed as:
1, plumbous detection kit 2, heavy metal lead detector 3, signal conditioning package 4, plumbous reagent 5, injector 6, sample hose 7, quartzy micro-cuvette 8, light source 9, monochromator 10, colorimetric pool 11, monochromator 12, detecting device 13, entrance slit 14, quasi-optical device 15, dispersion element 16, beam condensing unit 17, the exit slit of detecting
Embodiment
Referring to accompanying drawing 1-4, the equipment of this real-time constant-temperature quantitative fast detecting lead, comprises the timing temp controller (1) connecting successively, plumbous detection kit (2), heavy metal lead detector (3), plumbous detection kit comprises the plumbous reagent 4 that detects, reagent reacting pipe 5, injector 6, quartzy micro-cuvette 7; Heavy metal lead detector 2 comprises light source 8, the first monochromators 9 that connect successively, colorimetric pool 10, the second monochromators 11, detecting device 12; Monochromator is by entrance slit 13, quasi-optical device 14, dispersion element 15, and beam condensing unit 16, exit slit 17 forms.
Testing sample is placed in timing temp controller hole slot, 37 degrees Celsius of constant temperature are cultivated 5 minutes, testing sample is carried out to constant temperature pre-service, start heavy metal lead detector, micro-quartz cuvette is put into checkout equipment, carry out preheating, draw sample liquid with injector, injector end is connected with the built-in plumbous reagent reacting pipe that detects reagent, and the sample reagent reacting pipe of flowing through, is injected in quartzy micro-cuvette.Because detecting in reagent 4, lead comprises a kind of specific substrate and a kind of fluorophor, this fluorophor is connected on this specific substrate, forms fluorescence labeling specific substrate, and the lead in sample detects reagent at this lead and does the used time, fluorophor is released, thereby causes signal intensity.After testing sample and detection reagent mix, react, generation fluorescence signal changes, monitored by heavy metal lead detector, light source is entered by entrance slit 13, be that lens transfer directional light to through quasi-optical device 14, be that prism or grating decompose complex light by dispersion element 15, be that the monochromatic light of separation is focused to exit slit 17 outgoing by lens by focalizer 16 again, relatively change in fluorescence, and detection data are sent to signal conditioning package, signal conditioning package analysis of fluorescence signal intensity data, at the lead content of display screen show sample.
Equipment Inspection object:
Can be directly used in Measurement accuracy potable water and have natural surface water or the underground water sample of many interfering materials, also can be used for matrix, seawater, sewage and the solid sample of Analysis of Complex as samples such as rock, soil sample, biologies.
Technical properties of plant is as follows:
1, detect lower limit: 0.5ng/ml.
2, sounding lead range: 0-200ng/ml, can suitably dilute for the sample of higher concentration.
3, sample size: 0.1-0.5ml sample
4, water resistance: water resistance is good
Embodiment 1: detect certain industrial waste water lead content
Sample pre-treatments:
Get 50 milliliters of water samples, after filtering, dilute 100 times, regulate pH to be about 5.
Determination step:
Start real-time quantitative fluorescence heavy metal lead fast detector, micro-quartz cuvette is inserted in instrument, carry out preheating.Draw 0.5 ml sample liquid with injector, injector is inserted on reagent reacting pipe, be expelled in quartzy micro-cuvette by reagent reacting pipe, rapid moving is driven injector, and covers the lid of sample chamber, press beginning key and start test, sample starts after detection, within 1 minute, can show the test result take ppb as unit, records testing result, after having tested, micro-quartz cuvette is taken away.
Result: it is 150ppb that the equipment by real-time constant-temperature quantitative fast detecting lead and method detect this Pb In Exhausted Water content, and ICP-MS testing result is 145ppb.Real-time quantitative fluorescence heavy metal lead method for quick of the present invention and ICP-MS testing result are basically identical.
Embodiment 2: Site Detection somewhere tap water lead content
Determination step:
Start real-time quantitative fluorescence heavy metal lead fast detector, micro-quartz cuvette is inserted in instrument, carry out preheating.Draw 0.5 ml tap water sample with injector, injector is inserted on reagent reacting pipe, be expelled in quartzy micro-cuvette by reagent reacting pipe, rapid moving is driven injector, and covers the lid of sample chamber, press beginning key and start test, sample starts after detection, within 1 minute, can show the test result take ppb as unit, records testing result, after having tested, micro-quartz cuvette is taken away.
Result: this tap water sample, does not detect lead by equipment and the method for real-time constant-temperature quantitative fast detecting lead.It is 0 that ICP-MS detects lead content, and plumbous detection reagent testing result is consistent with ICP-MS testing result.
The present invention uses synthetic biology albumen or DNA specific site labelling technique, Dynamics of Enzyme Catalysis, in conjunction with photoelectric detecting technology and automatic control module fractional analysis system, real-time quantitative detects lead content, has guaranteed stability, sensitivity and the specificity of system, has realized accurate detection.Can be applicable to the fast detecting such as the chemical residue such as heavy metal, toxin, the examination of pathogenic microorganisms drug resistance.Can also help enterprise to monitor in real time physics and chemistry residue, the microbes contamination of starting material, environment, finished product, find in time potential pollution situation, reduce risk, can improve business capital running rate, promote the expansion of turnout, particularly should rotten product for the fugitive phase, goods putting can be accelerated whole logistics chain fast, controls and reduces enterprise-quality security risk.
Obviously, above-described embodiment is only for example is clearly described, and the not restriction to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also giving exhaustive to all embodiments.And the apparent variation of being extended out thus or variation are still among the protection domain in the invention.
Claims (19)
1. the equipment of a real-time constant-temperature quantitative fast detecting lead, it is characterized in that, comprise the timing temp controller (1) connecting successively, plumbous detection kit (2), heavy metal lead detector (3), after testing sample is cultivated in timing temp controller, with the generation signal intensity that reacts after detection reagent mix, heavy metal lead detector is monitored this signal intensity under steady temperature, and detection data are sent to built-in signal conditioning package, this signal conditioning package, to detecting data analysis, obtains sample lead content.
2. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 1, is characterized in that, comprises a kind of timing temp controller (1).
3. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 2, is characterized in that, the timing temp controller (1) comprising, and accurately the temperature of Quality control is 37 degrees Celsius.
4. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 3, is characterized in that, comprises a kind of plumbous detection kit (2), and kit comprises a kind of plumbous reagent (4) that detects, and is placed in reagent reacting pipe (5).
5. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 4, it is characterized in that, described in right 4 detection reagent (4) in contain special alpha-non-natural amino acid fluorescence labeling substrate, in the time not containing lead in sample, do not send fluorescence signal, in the time that the lead in sample and this substrate are had an effect, fluorophor is released, and causes signal intensity.
6. according to the equipment of a kind of real-time constant-temperature quantitative fast detecting lead one of claim 2-5 Suo Shu, it is characterized in that, described plumbous detection kit also comprises injector (6), quartzy micro-cuvette (7).
7. according to the equipment of a kind of real-time constant-temperature quantitative fast detecting lead one of claim 1-6 Suo Shu, it is characterized in that, described heavy metal lead detector (3) comprises the light source (8) connecting successively, the first monochromator (9), colorimetric pool (11), the second monochromator (10), detecting device (12), thermostat (18).
8. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 7, it is characterized in that, described monochromator is by entrance slit (13), quasi-optical device (14), dispersion element (15), beam condensing unit (16), exit slit (17) composition.
9. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 8, is characterized in that, described quasi-optical device and beam condensing unit are lens.
10. the equipment of a kind of real-time constant-temperature quantitative fast detecting lead according to claim 9, is characterized in that, described dispersion element is prism or grating.
The equipment of one of 11. according to Claim 8-10 described a kind of real-time constant-temperature quantitative fast detecting lead, is characterized in that, described heavy metal lead detector also comprises LED display, for showing detection data.
The equipment of 12. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 7, it is characterized in that, described heavy metal lead detector also comprises a kind of built-in thermostat (18), can accurately control the temperature of reaction system and carry out under 37 degrees Celsius.
The equipment of 13. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 12, is characterized in that, described heavy metal lead detector also comprises USB interface.
The method of 14. 1 kinds of real-time constant-temperature quantitative fast detecting lead, is characterized in that, comprises the following steps:
S1, testing sample is placed in timing temp controller (1) hole slot, 37 degrees Celsius of constant temperature are cultivated 5 minutes, and testing sample is carried out to constant temperature pre-service;
S2, startup heavy metal lead detector, put into checkout equipment by micro-quartz cuvette (7), is preheating to 37 degrees Celsius;
S3, draw sample liquid with injector (6), injector end is connected with the reagent reacting pipe (5) of built-in plumbous detection reagent (4), the sample reagent reacting pipe (5) of flowing through, is injected in quartzy micro-cuvette (7);
S4, remove reagent reacting pipe (5) and injector (6), cover the lid of sample test cup;
S5, press start key start test, sample with detect reagent (4) effect, occur signal intensity, in 2 minutes, show testing result, detection completes.
The method of 15. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 14, is characterized in that, the pre-service of described testing sample is 30 degree constant temperature 5 minutes, carries out under same temperature to ensure to react.
The method of 16. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 15, it is characterized in that, described lead detects reagent (4) and comprises a kind of specific substrate and a kind of alpha-non-natural amino acid fluorophor, this fluorophor is connected on this specific substrate, forms alpha-non-natural amino acid fluorescence labeling substrate.
The method of 17. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 16, is characterized in that, described lead detects reagent (4) and also comprises a kind of stabilizing agent, ensures the stability of reaction system.
The method of 18. a kind of real-time constant-temperature quantitative fast detecting lead according to claim 17, is characterized in that, substrate fluorophor is quenched, and does the used time when the lead in sample and this lead detect reagent, and fluorophor is released, and causes signal intensity.
19. according to the method for a kind of real-time constant-temperature quantitative fast detecting lead one of claim 14-18 Suo Shu, it is characterized in that, it is 0.5ml that described injector (5) is drawn sample size in sample hose.
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Cited By (3)
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| CN106153587A (en) * | 2016-06-21 | 2016-11-23 | 何文 | A kind of detection method of soil activation state cadmium |
| CN109164092A (en) * | 2018-09-12 | 2019-01-08 | 淮安安福科技有限公司 | The fast quantitative measurement method for detecting and device of specific viable bacteria in a kind of sample |
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| CN105866093A (en) * | 2016-06-21 | 2016-08-17 | 何文 | Detection method of soil active-state lead |
| CN106153587A (en) * | 2016-06-21 | 2016-11-23 | 何文 | A kind of detection method of soil activation state cadmium |
| CN109164092A (en) * | 2018-09-12 | 2019-01-08 | 淮安安福科技有限公司 | The fast quantitative measurement method for detecting and device of specific viable bacteria in a kind of sample |
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Inventor after: Zhou Juanzuo Inventor after: Lu Yi Inventor after: Huang Fang Inventor after: Wang Jiangyun Inventor after: Jin Jinghua Inventor before: Zhou Juanzuo Inventor before: Lu Yi Inventor before: Wang Jiangyun Inventor before: Jin Jinghua |
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