CN103849579B - 净化水质用自然循环微生物菌剂及其制备方法 - Google Patents
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- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
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Abstract
本发明公开了净化水质用自然循环微生物菌剂及其制备方法,由闪杆菌属(Allochromatium)、闪囊菌属(Lamprocystis)、嗜盐红螺旋菌属(Halorhodospira)、着色菌属(Chromatium)、亚硝化毛杆菌属(Nitrosomonas)、硝酸细菌属(Nitrobacter)、亚硝酸菌属(Nitrosomonas)和假单胞菌属(Pseudomonas)8种细菌的发酵液组成,以上8种细菌在发酵液中的有效活菌数分别都≧1.0×108CFU/ml。本发明的微生物菌剂对各种污水、废水都有较好的净化作用。
Description
技术领域
本发明属于环保技术领域,涉及一种微生物菌剂,具体涉及一种净化水质用自然循环微生物菌剂及其制备方法。
背景技术
近几十年以来,随科学技术、社会经济迅速发展带来了环境严重污染和生态破坏。微生物降解污染物的巨大潜力在控制污染、修复环境中发挥重要作用。人类生产和生活活动产生的污水(废水)、废气及固体废弃物都可以用生物方法进行处理。现在技术中,还缺乏一种对各种生产、生活废水的都具有良好水质净化作的微生物菌剂。
发明内容
本发明的目的就是针对现有技术的不足,提供一种净化水质用自然循环微生物菌剂。
本发明另外提供一种净化水质用自然循环微生物菌剂的制备方法。
为了实现上述目的,本发明提出如下技术方案:
一种净化水质用自然循环微生物菌剂,该微生物菌剂由闪杆菌属(Allochromatium)、闪囊菌属(Lamprocystis)、嗜盐红螺旋菌属(Halorhodospira)、着色菌属(Chromatium)、亚硝化毛杆菌属(Nitrosomonas)、硝酸细菌属(Nitrobacter)、亚硝酸菌属(Nitrosomonas)和假单胞菌属(Pseudomonas)8种细菌的发酵液组成,以上8种细菌在发酵液中的有效活菌数分别都≧1.0×108CFU/ml。
优选的,所述闪杆菌属(Allochromatium)为嗜中盐闪杆菌(Lamprobactermodestohalophilus);所述闪囊菌属(Lamprocystis)为桃红色闪囊菌(Lamprocystisroseopersicina);所述嗜盐红螺旋菌属(Halorhodospira)为嗜盐嗜盐红螺菌(Halorhodospirahalophila);所述着色菌属(Chromatium)为奥氏着色菌(Chromatiumokenii)或酒色着色菌(Chromatiumvinosum);所述亚硝化毛杆菌属(Nitrosomonas)为欧洲亚硝化毛杆菌(Nitrosomonaseuropaea);所述硝酸细菌属(Nitrobacter)为维氏硝酸细菌(Nitrobacterwinogradskyi)或活跃硝酸细菌(N.agilis);所述亚硝酸菌属为亚硝化单胞菌(Nitrosomonaseutropha)或亚硝化球菌(Nitrosococcussp.);所述假单胞菌属(Pseudomonas)为恶臭假单胞菌(Pseudomonasputida)。
优选的,所述发酵液所用的培养基为每1L水中加入0.5g磷酸二氢钾(KH2PO4)、0.2g七水硫酸镁(MgSO47H2O)、0.4g氯化钠(NaCI)、1.2g氯化铵(NH4CI)、0.05g二水合氯化钙(CaCI22H2O)、0.3g酵母提取物(yeastextract)、1ml矿液(mineralsolution(每1L中含有25%(v/v)氯化氢(HCI)1ml、氯化锌(ZnCI2)70mg、四水氯化锰(MnCI24H2O)100mg、硼酸(H3BO3)60mg、氯化钴(CoCI26H2O)200mg、氯化铜(CuCI2H2O)20mg、钼酸钠(NaMoO42H2O)40mg))充分溶化后,水溶液的PH调节到7.0,进行高压灭菌处理。
本发明的净化水质用自然循环微生物菌剂的制备方法,步骤如下:
(1)培养基的制造
本培养液的制造方法如下,每1L水中加入0.5g磷酸二氢钾(KH2PO4)、0.2g七水硫酸镁(MgSO47H2O)、0.4g氯化钠(NaCI)、1.2g氯化铵(NH4CI)、0.05g二水合氯化钙(CaCI22H2O)、0.3g酵母提取物(yeastextract)、1ml矿液(mineralsolution(每1L中含有25%(v/v)氯化氢(HCI)1ml、氯化锌(ZnCI2)70mg、四水氯化锰(MnCI24H2O)100mg、硼酸(H3BO3)60mg、氯化钴(CoCI26H2O)200mg、氯化铜(CuCI2H2O)20mg、钼酸钠(NaMoO42H2O)40mg))充分溶化后,水溶液的PH调节到7.0,进行高压灭菌处理。
(2)培养基准
将上面的培养基5L、8种等量的原菌放入装有100L水的透明玻璃容器中维持水的温度在36-40℃,距离水面12-18cm用4个36w三波长灯泡进行照明,培养6-8天。
上述方法中,优选的,所述步骤(2)中水的温度为38℃,距离水面15cm,培养7天。
上述方法中,优选的,所述放入的原菌为8种菌株经步骤(1)所述培养基培养后经检测菌落数量≧1.0×108CFU/ml后的菌液,8种菌珠的菌液共5L。
本发明微生物菌剂的用途及适用范围
用途 | 适用范围 |
高浓度、低浓度废水的水质净化处理 | 垃圾处理厂污水、人粪尿、养猪废水、皮革废水、屠宰废水、化工废水、金属废水、染色废水、造纸废水、食品废水、水产加工废水、家庭饮食生活废水等 |
本发明微生物菌剂的使用量及使用方法
废水种类 | 废水量 | 需投入自然循环微生物菌剂的量 |
1.人粪废水/养猪废水 | 30-40吨 | 约20kg |
2.垃圾处理厂污水 | 2000-10000吨 | 约20kg |
3.皮革废水 | 100-500吨 | 约20kg |
4.染色/造纸废水 | 1000-5000吨 | 约20kg |
5.屠宰废水 | 100-500吨 | 约20kg |
6.生产食品废水(泡菜、豆腐等) | 200-1000吨 | 约20kg |
7.水产加工废水 | 100-500吨 | 约20kg |
8.家庭饮食生活废水 | 20-50吨 | 约20kg |
9.堤坝/河流/湖(蓄水池)等 | 根据富营养化的程度调节 | 约20kg |
上述使用量以及使用方法根据废水发生地以及废水的组成有显著地差异。本发明的微生物菌剂对各种污水、废水都有较好的净化作用。
本发明的附加方面和优点将在下面的描述中部分给出,部分将从下面的描述中变得明显,或通过本发明的实践了解到。
具体实施方式
下面通过实施例对本发明的技术方案作进一步说明,但不能理解为对本发明的限制。
实施例1
一种用于净化水质的自然循环微生物菌剂,品质基准:
经实验,本发明所涉及菌株均为公知菌株,且采用该菌珠的菌剂均具有相同的水质净化效果。
所述的净化水质用自然循环微生物菌剂的制备方法,步骤如下:
(1)培养基的制造
本培养液的制造方法如下,每1L水中加入0.5g磷酸二氢钾(KH2PO4)、0.2g七水硫酸镁(MgSO47H2O)、0.4g氯化钠(NaCI)、1.2g氯化铵(NH4CI)、0.05g二水合氯化钙(CaCI22H2O)、0.3g酵母提取物(yeastextract)、1ml矿液(mineralsolution(每1L中含有25%(v/v)氯化氢(HCI)1ml、氯化锌(ZnCI2)70mg、四水氯化锰(MnCI24H2O)100mg、硼酸(H3BO3)60mg、氯化钴(CoCI26H2O)200mg、氯化铜(CuCI2H2O)20mg、钼酸钠(NaMoO42H2O)40mg))充分溶化后,水溶液的PH调节到7.0,进行高压灭菌处理。
(2)培养基准
将上面的培养基5L、8种原菌等量菌液共5L放入装有100L水的透明玻璃容器(水族箱)中维持水的温度在约38℃,距离水面约15cm用4个36w三波长灯泡进行照明,培养约7天。
实验例1:废水的水质净化实验结果
将垃圾处理厂污水、人粪废水、养猪废水、皮革废水各取20L分别装入圆柱形水槽中,分别投入实施例1制备的自然循环微生物菌剂0.2、10、10、4ml后搅拌,分别过4小时、1天、1天、8小时添加自然循环微生物菌剂。为了检测水质净化处理的效果,在不同时间测试BOD、COD、SS、T-N、T-P。
(1)垃圾处理厂污水
时间(hour) | BOD(ppm) | COD(ppm) | SS(ppm) | T-N(ppm) | T-P(ppm) |
0 | 138.8 | 156.5 | 135.3 | 25.6 | 2.489 |
15 | 8.5 | 7.8 | 8.3 | 8.0 | 0.330 |
24 | 4.3 | 4.5 | 5.0 | 4.1 | 0.101 |
(2)人粪废水
时间(day) | BOD(ppm) | COD(ppm) | SS(ppm) | T-N(ppm) | T-P(ppm) |
0 | 24,000 | 13,570 | 20,800 | 5,560 | 780.0 |
3 | 3,590 | 1,678 | 3,219 | 346 | 69 |
6 | 120 | 90 | 110 | 54 | 5.3 |
10 | 11 | 25 | 13 | 15 | 1.5 |
(3)养猪废水
时间(day) | BOD(ppm) | COD(ppm) | SS(ppm) | T-N(ppm) | T-P(ppm) |
0 | 22,000.0 | 18,700.0 | 25,000.0 | 3,500.0 | 480.0 |
3 | 1,055.0 | 680.0 | 1,211.0 | 900.5 | 65.4 |
5 | 140.3 | 100.1 | 145.9 | 45.2 | 15.0 |
10 | 9.8 | 7.3 | 10.9 | 8.4 | 1.0 |
(4)皮革废水
时间(day) | BOD(ppm) | COD(ppm) | SS(ppm) | T-N(ppm) | T-P(ppm) |
0 | 2,300.0 | 1,850.0 | 3,800.0 | 415.0 | 16.0 |
1 | 1,432.0 | 899.2 | 1,678.2 | 175.4 | 10.8 |
3 | 112.5 | 81.3 | 80.3 | 42.5 | 3.5 |
5 | 8.9 | 9.1 | 10.1 | 10.6 | 0.3 |
废水的净化作用原理
检测基准
(1)取1L上述所讲的培养基加入1-3g琼脂(agar)搅拌均匀、高压灭菌后,放在培养碟(petridish)中凝固。
(2)在冷却的培养碟中均匀喷洒100倍或1000倍稀释后的自然循环微生物菌剂。
(3)培养碟上方10cm处放36W三波长灯泡照明,保持温度在约38℃,培养约7天后可以检测着色菌的菌落数量。
(4)每种着色菌菌落数量≧1.0×108CFU/ml。
Claims (4)
1.一种净化水质用自然循环微生物菌剂,其特征在于,该微生物菌剂由闪杆菌属(Lamprobacter)、闪囊菌属(Lamprocystis)、嗜盐红螺旋菌属(Halorhodospira)、着色菌属(Chromatium)、亚硝化单胞菌属(Nitrosomonas)中的两个不同种、硝酸细菌属(Nitrobacter)和假单胞菌属(Pseudomonas)8种细菌的发酵液组成,以上8种细菌在发酵液中的有效活菌数分别都≧1.0×108CFU/ml;
所述闪杆菌属(Lamprobacter)为嗜中盐闪杆菌(Lamprobactermodestohalophilus);所述闪囊菌属(Lamprocystis)为桃红色闪囊菌(Lamprocystisroseopersicina);所述嗜盐红螺旋菌属(Halorhodospira)为嗜盐嗜盐红螺菌(Halorhodospirahalophila);所述着色菌属(Chromatium)为奥氏着色菌(Chromatiumokenii)或酒色着色菌(Chromatiumvinosum);所述亚硝化单胞菌属(Nitrosomonas)为欧洲亚硝化单胞菌(Nitrosomonaseuropaea)和亚硝化单胞菌Nitrosomonaseutropha;所述硝酸细菌属(Nitrobacter)为维氏硝酸细菌(Nitrobacterwinogradskyi)或活跃硝酸细菌(Nitrobacteragilis);所述假单胞菌属(Pseudomonas)为恶臭假单胞菌(Pseudomonasputida);
所述发酵液所用的培养基为每1L水中加入0.5g磷酸二氢钾(KH2PO4)、0.2g七水硫酸镁(MgSO4·7H2O)、0.4g氯化钠(NaCl)、1.2g氯化铵(NH4Cl)、0.05g二水合氯化钙(CaCl2·2H2O)、0.3g酵母提取物(yeastextract)、1ml矿液(mineralsolution),所述矿液每1L中含有25%(v/v)氯化氢(HCl)1ml、氯化锌(ZnCl2)70mg、四水氯化锰(MnCl2·4H2O)100mg、硼酸(H3BO3)60mg、六水合氯化钴(CoCl2·6H2O)200mg、一水合氯化铜(CuCl2·H2O)20mg、二水合钼酸钠(NaMoO4·2H2O)40mg,充分溶化后,水溶液的PH调节到7.0,进行高压灭菌处理,得培养基母液,将培养基母液5L放入100L水中即得。
2.权利要求1所述的净化水质用自然循环微生物菌剂的制备方法,其特征在于,步骤如下:
(1)培养基的制造
本培养基的制造方法如下,每1L水中加入0.5g磷酸二氢钾(KH2PO4)、0.2g七水硫酸镁(MgSO4·7H2O)、0.4g氯化钠(NaCl)、1.2g氯化铵(NH4Cl)、0.05g二水合氯化钙(CaCl2·2H2O)、0.3g酵母提取物(yeastextract)、1ml矿液(mineralsolution),所述矿液每1L中含有25%(v/v)氯化氢(HCl)1ml、氯化锌(ZnCl2)70mg、四水氯化锰(MnCl2·4H2O)100mg、硼酸(H3BO3)60mg、六水合氯化钴(CoCl2·6H2O)200mg、一水合氯化铜(CuCl2·H2O)20mg、二水合钼酸钠(NaMoO4·2H2O)40mg,充分溶化后,水溶液的PH调节到7.0,进行高压灭菌处理;
(2)培养基准
将上面的培养基5L、8种等量的原菌放入装有100L水的透明玻璃容器中维持水的温度在36-40℃,距离水面12-18cm用4个36w三波长灯泡进行照明,培养6-8天。
3.根据权利要求2所述的方法,其特征在于,所述步骤(2)中水的温度为38℃,所述三波长灯泡距离水面15cm,培养7天。
4.根据权利要求2所述的方法,其特征在于,所述放入的原菌为8种菌株经步骤(1)所述培养基培养后经检测菌落数量≧1.0×108CFU/ml后的菌液,8种菌株的菌液共5L。
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