CN103845276A - Bacteriorhodopsin biological gel and preparation method thereof - Google Patents
Bacteriorhodopsin biological gel and preparation method thereof Download PDFInfo
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- CN103845276A CN103845276A CN201210495597.2A CN201210495597A CN103845276A CN 103845276 A CN103845276 A CN 103845276A CN 201210495597 A CN201210495597 A CN 201210495597A CN 103845276 A CN103845276 A CN 103845276A
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- bacteria rhodopsin
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Abstract
The present invention relates to a bacteriorhodopsin (BR) biological gel and a preparation method thereof. According to the present invention, a polymer compound with thickening property is suspended in water to form a gel substrate for skin so as to provide viscosity, and the gel substrate is combined with a BR culture solution obtained through halophilic bacteria culture to prepare the BR biological gel, wherein the BR biological gel comprises beneficial vitamins, minerals, amino acids and the like, can be applied in skin beautifying and treatment, and can be expected to develop great commercial potential in a plurality of fields.
Description
Technical field
The present invention relates to bacteria rhodopsin, particularly relate to a kind of bacteria rhodopsin biogel and preparation method thereof.
Background technology
Why interested in this quasi-microorganism of halophilic bacteria (halobacteria) people are, mainly because of purple cell membrane (the purple membrane from halophilic bacteria, PM) in, contain with vision in the similar protein of rhodopsin, and in purple cell membrane, only has this kind of unique protein, so be called bacteria rhodopsin (bacteriorhodopsin, BR).Bacteria rhodopsin has single direction light proton pump (light-drivenproton pump) function of tending to act, both can utilize luminous energy synthesizing adenosine triphosphoric acid (Adenosine triphosphate, ATP), be similar to photosynthetic function, also can in unglazed situation, carry out oxidative phosphorylation, carry out bacterial growth breeding.Because bacteria rhodopsin has unique 26S Proteasome Structure and Function in purple cell membrane, be acknowledged as the simplest light energy conversion biomolecule the chlorophyll in green plants, and there is one of green light electric material of application potential most.Its application category is extremely wide, such as artificial retina, three-dimensional optical internal memory, photoswitch, photoelectric sensor, as many-sides such as the exploitations of edge detector, the dull filtering system of optical imagery, anti-fake material, biochip and raw matter Hydrogen Energy and fuel cell.
Concentrate on studies all of this halophilic bacteria of the application's case inventor, especially about in the research by cultivating the bacterial rhodopsin that these halophilic bacterias produce and application exploitation, we have learnt that the culture fluid for producing bacterial rhodopsin is very useful, the liquid of the composition such as its useful vitamin, mineral, aminoacid, can, not only as a part for any cosmetics, also can in other application, use.
Summary of the invention
In view of this, main order of the present invention is to propose a kind of bacteria rhodopsin biogel and preparation method thereof, be that bacteria rhodopsin culture fluid is incorporated into the gel substrate with vicidity, the physiological function benefit that bacteria rhodopsin culture fluid is produced, the pathological changes that can be used to promote the function of skin or improve some skins has greatest help for the common people.
For achieving the above object, the present invention proposes a kind of bacteria rhodopsin biogel, include gel substrate and bacteria rhodopsin culture fluid, wherein gel substrate is to be mixed in water and to be formed by the macromolecular compound with thickening property, bacteria rhodopsin culture fluid is scattered in gel substrate, and bacteria rhodopsin culture fluid comprises by cultivating the bacteria rhodopsin that halophilic bacteria produces in exhaustion culture fluid.
In addition, the present invention proposes a kind of preparation method of bacteria rhodopsin biogel, its step includes: first, obtain gel substrate by the step that the macromolecular compound with thickening property is mixed in water, then, by by exhausting that cultivating the bacteria rhodopsin culture fluid that halophilic bacteria obtains in culture fluid adds in gel substrate, can make bacteria rhodopsin biogel of the present invention.
Because bacteria rhodopsin culture fluid comprises many nutrient substance, such as vitamin, mineral and aminoacid etc., not only contribute to keep the health of skin with young, and have the effect of preventing and improving disease.In addition, in specific embodiment, macromolecular compound can be Carbopol 980 NF or xanthan gum, xanthan gum also can carry out combination with other aqueous colloidal, for example carob, can make bacteria rhodopsin biogel can keep suitable vicidity and become the gel dosage form being suitable for use on skin, moreover, this bacteria rhodopsin biogel is not only made fast, do not block pore, can rapidly and be evenly distributed on skin, and can add easily other composition, for example, can further add essence or hydrophilic pharmaceutical substance, offer product that meets demand and there is superperformance of consumer.
Therefore, bacteria rhodopsin biogel provided by the present invention, is expected to become a breakthrough medical cosmetology product, and can, in response to different users's demand, develop other function, has great business potential.
Under by specific embodiment coordinate Figure of description illustrate in detail so that the effect that is easier to understand object of the present invention, technology contents, feature and reaches.
Brief description of the drawings
Fig. 1 is the flow chart of the preparation method of bacteria rhodopsin biogel of the present invention.
Detailed description of the invention
The flow chart of the preparation method of disclosed bacteria rhodopsin biogel according to the present invention, as shown in Figure 1.First, carry out step S10, one gel substrate is provided, this gel substrate is to suspend in water prepared by the macromolecular compound with thickening property substantially, and macromolecular compound can be for example Carbopol 980 NF (Carbopol) or xanthan gum (Xanthan), below for how to prepare gel substrate of the present invention with these two kinds of macromolecular compounds be illustrated respectively.
(1) prepare gel substrate by Carbopol 980 NF:
A. first, Carbopol 980 NF is weighed.The content of Carbopol 980 NF be gel substrate 0.2 to 0.5wt%(, in every 100 grams of gel substrates, have the Carbopol 980 NF of 0.2 to 0.5 gram).
B. Carbopol 980 NF is sieved and add in low pH value cold water, wait for quietly, about one day, making Carbopol 980 NF imbibition.
C. obtained solution is carried out to the Homogenization Process of 30 minutes.
D. add nertralizer, constantly stir with 500 revs/min, carry out N-process (neutralizationstep), the pH value of regulator solution reaches between 6 to 7, to obtain the clear gel with high viscosity and applications well characteristic, i.e. gel substrate of the present invention.
Nertralizer, can be selected from group's combination of alkali (alkali), sodium bicarbonate (soda), carbonate (carbonate), hydrogen ammonium carbonate (ammonium hydrogen carbonate), ammonia (ammonia), triethanolamine (triethanolamine), sodium hydroxide (NaOH) and potassium hydroxide (KOH).
Wherein, if use triethanolamine, the ratio of Carbopol 980 NF and triethanolamine is 1.0:1.5.If use hydroxide (sodium hydroxide or potassium hydroxide), the ratio of Carbopol 980 NF and sodium hydroxide or potassium hydroxide is 1.0:2.3.
Above-mentioned N-process is very important.Owing to there being a free carboxy (carboxyl group ,-COOH) molecule in Carbopol 980 NF used in the present invention, the pH value that makes the aqueous solution that is distributed with Carbopol 980 NF is 3 to 4.When pH value reaches while being greater than 6, the macromolecular chain of the tight irregular winding of Carbopol 980 NF just can start charged and mutual exclusion to be broken up and comes, and strand is entangled with and is reticulated structure and viscosity is increased.Therefore, by N-process, will be that very small amount of nertralizer adds with respect to water, can make mixture retrogradation to form sticky gel.
(2) prepare gel substrate by xanthan gum:
A. xanthan gum is weighed.Xanthan gum, in low concentration, can be just very sticky gel, its use content be probably gel substrate 0.5 to 2wt%.
B. water is heated to the temperature of approximately 36 to 38 DEG C.Xanthan gum all can dissolve well in hot water and cold water, but at this temperature, its dissolution velocity can be faster.
C. to allow xanthan gum easily mix thoroughly in water and not form lump, and preferably can easily separate (dispersion).Accomplish this point, just must fully mix with other composition, then add water.It can be dry composition, as corn starch, clay or oatmeal.Or, also can, in nonaqueous solvent, as glycerol, ethanol or vegetable oil (being several), xanthan gum be mixed, wait for one or two minute quietly, then stir.
D. in water, add xanthan gum, and strong stirring, to make gel substrate.
In addition, can in gel substrate, add other composition, can first other composition be added in water, then add xanthan gum.Also can add antiseptic, in order to preservation.Moreover, also can use xanthan gum in conjunction with other aqueous colloidal, such as carob.
After said process, just can obtain gel substrate, and any other composition of use of can arranging in pairs or groups, for example adding essence provides fragrance, or further adds hydrophilic pharmaceutical substance, and a product that meets consumer demand can be provided by this.
Then, we use it to complete the preparation of bacteria rhodopsin biogel in conjunction with bacteria rhodopsin culture fluid, as the step S20 of Fig. 1.And the preparation of this bacteria rhodopsin culture fluid is by cultivating halophilic bacteria and produce bacteria rhodopsin exhausting in (spent) culture fluid, and this liquid that includes bacteria rhodopsin desired bacteria rhodopsin culture fluid that is exactly us.
The present invention is to be applied to treatment psoriasis (psoriasis) as example, we use 25% sodium chloride solution as exhausting that culture fluid is (in practical application, exhaust that culture fluid can be the sodium chloride solution of concentration 26 to 31%), carry out the cultivation of halophilic bacteria and the production of bacteria rhodopsin, and the living cells content of bacteria rhodopsin culture fluid sample is 0.3 to 4 grams per liter, we pour this bacteria rhodopsin culture fluid and gel substrate in a container into, mix 5 minutes, make color even, complete the preparation of bacteria rhodopsin biogel, as step S30.
Table one to table four is the chromatography result of bacteria rhodopsin culture fluid composition in example of the present invention.
Table monoamino-acid (amino acid) composition
| Aminoacid | Content (mg/litre) |
| Aspartic acid (Aspartic acid) | 134.4 |
| Glutamic acid (Glutamic acid) | 2.2 |
| Serine (serine) | 27,3 |
| Histidine (histidine) | 120,4 |
| Glycine (glycine) | 101,6 |
| Threonine (threonine) | 15,7 |
| Arginine (arginine) | 15,5 |
| Alanine (alanine) | 220,3 |
| Tyrosine (tyrosine) | 103,4 |
| Valine (valine) | 136,8 |
| Methionine (methionine) | 19,7 |
| Tryptophan (tryptophan) | 23,0 |
| Phenylalanine (phenylalanine) | 37,9 |
| Isoleucine (isoleucine) | 35,7 |
| Leucine (leucine) | 58,2 |
| Lysine (lysine) | 414,3 |
| Proline (proline) | 61,3 |
Table two active metal cation constituent
| Cation | Content (mg/litre) |
| Lithium ion (Li +） | N/A |
| Ammonium radical ion (NH 4 +） | N/A |
| Potassium ion (K +） | 42,81 |
| Calcium ion (Ca 2+） | 6,85 |
| Magnesium ion (Mg 2+） | 21,72 |
Table three Trace Elements (being no more than)
| Trace element | Content (mg/litre) |
| Copper (Cu) | 0,009 |
| Ferrum (Fe) | 0,191 |
| Manganese (Mn) | 0,0165 |
| Phosphorus (P) | 59,5 |
| Sulfur (S) | 80,36 |
| Zinc (Zn) | 0,411 |
Table four vitamin composition (being no more than)
| Vitamin | Content (mg/litre) |
| B7(H) | 2,398 |
| Choline chloride (choline chloride) | 2665,4 |
| B12 | 0,1742 |
| Folic acid (folic acid) | 146,665 |
| B8 | 7365 |
| B3(PP) | 3161 |
| B5 | 659，35 |
| B10 | 211,45 |
| B6 | 185,585 |
| B2 | 738,7 |
| B1 | 42,2 |
Combine it, the disclosed bacteria rhodopsin biogel of the present invention is very useful, by bacteria rhodopsin vitamin that culture fluid comprises, mineral, aminoacid etc. useful nutrient substance, can be used as a part for any medical cosmetology product, keep the health of skin with young by contributing to, and can improve the pathological changes of some skins, or, also can in other application, use, for the common people, have greatest help.
Moreover, this bacteria rhodopsin biogel is for keeping suitable vicidity to be suitable for use in the gel dosage form on skin, and make fast, do not block pore, can rapidly and be evenly distributed on skin, also can add easily other composition, for example, can further add essence or hydrophilic pharmaceutical substance, offer product that meets demand and there is superperformance of consumer.
Therefore, in the near future, bacteria rhodopsin biogel provided by the present invention, is expected to become a breakthrough medical cosmetology product, and can, in response to different users's demand, develop other function, has great business potential.
Although the present invention discloses as above with aforesaid embodiment, so it is not in order to limit the present invention.Without departing from the spirit and scope of the present invention, the change of doing and retouching, all belong to scope of patent protection of the present invention.
Claims (27)
1. a bacteria rhodopsin biogel, it comprises:
One gel substrate is to be mixed in water and to be formed by the macromolecular compound with thickening property; And
One bacteria rhodopsin (bacteriorhodopsin, BR) culture fluid, is scattered in this gel substrate, and this bacteria rhodopsin culture fluid comprises by cultivating the bacteria rhodopsin that halophilic bacteria produces in an exhaustion (spent) culture fluid.
2. bacteria rhodopsin biogel as claimed in claim 1, wherein this macromolecular compound is Carbopol 980 NF (Carbopol).
3. bacteria rhodopsin biogel as claimed in claim 2, wherein the content of this Carbopol 980 NF be this gel substrate 0.2 to 0.5wt%.
4. bacteria rhodopsin biogel as claimed in claim 2, wherein this gel substrate also comprises the nertralizer of the group's combination that is selected from alkali (alkali), sodium bicarbonate (soda), carbonate (carbonate), hydrogen ammonium carbonate (ammoniumhydrogen carbonate), ammonia (ammonia), triethanolamine (triethanolamine), sodium hydroxide (NaOH) and potassium hydroxide (KOH), to neutralize.
5. bacteria rhodopsin biogel as claimed in claim 4, wherein the part by weight of this Carbopol 980 NF and this triethanolamine is 1.0:1.5.
6. bacteria rhodopsin biogel as claimed in claim 4, wherein the part by weight of this Carbopol 980 NF and this sodium hydroxide or this potassium hydroxide is 1.0:2.3.
7. bacteria rhodopsin biogel as claimed in claim 1, wherein this macromolecular compound is xanthan gum (Xanthan).
8. bacteria rhodopsin biogel as claimed in claim 7, wherein the content of this xanthan gum be this gel substrate 0.5 to 2wt%.
9. bacteria rhodopsin biogel as claimed in claim 7, wherein this gel substrate more comprises the dry composition of the group's combination that is selected from corn starch, clay and oatmeal, mixes this xanthan gum.
10. bacteria rhodopsin biogel as claimed in claim 7, wherein this gel substrate more comprises the nonaqueous solvent of the group's combination that is selected from glycerol, ethanol and vegetable oil, mixes this xanthan gum.
11. bacteria rhodopsin biogels as claimed in claim 7, wherein this macromolecular compound more comprises carob.
12. bacteria rhodopsin biogels as claimed in claim 1, wherein this exhaustion culture fluid is 26 to 31% sodium chloride solution.
13. bacteria rhodopsin biogels as claimed in claim 1, wherein this bacteria rhodopsin culture fluid keeps every liter and has the living cells of 0.3 to 4 gram.
The preparation method of 14. 1 kinds of bacteria rhodopsin biogels, its step comprises:
One gel substrate is provided, and this gel substrate is to obtain by the step that the macromolecular compound with thickening property is mixed in water; And
By a bacteria rhodopsin (bacteriorhodopsin, BR) culture fluid adds in this gel substrate to make this bacteria rhodopsin biogel, and this bacteria rhodopsin culture fluid is to produce by the step of cultivating halophilic bacteria and produce bacteria rhodopsin in an exhaustion (spent) culture fluid.
The preparation method of 15. bacteria rhodopsin biogels as claimed in claim 14, wherein this macromolecular compound is Carbopol 980 NF (Carbopol).
The preparation method of 16. bacteria rhodopsin biogels as claimed in claim 15, wherein the content of this Carbopol 980 NF be this gel substrate 0.2 to 0.5wt%.
The preparation method of 17. bacteria rhodopsin biogels as claimed in claim 15, after wherein this Carbopol 980 NF being mixed in to the step in water, more comprise the nertralizer that adds the group's combination that is selected from alkali (alkali), sodium bicarbonate (soda), carbonate (carbonate), hydrogen ammonium carbonate (ammonium hydrogen carbonate), ammonia (ammonia), triethanolamine (triethanolamine), sodium hydroxide (NaOH) and potassium hydroxide (KOH), neutralize.
The preparation method of 18. bacteria rhodopsin biogels as claimed in claim 17, wherein the part by weight of this Carbopol 980 NF and this triethanolamine is 1.0:1.5.
The preparation method of 19. bacteria rhodopsin biogels as claimed in claim 17, wherein the part by weight of this Carbopol 980 NF and this sodium hydroxide or this potassium hydroxide is 1.0:2.3.
The preparation method of 20. bacteria rhodopsin biogels as claimed in claim 14, wherein this macromolecular compound is xanthan gum (Xanthan).
The preparation method of 21. bacteria rhodopsin biogels as claimed in claim 20, wherein the content of this xanthan gum be this gel substrate 0.5 to 2wt%.
The preparation method of 22. bacteria rhodopsin biogels as claimed in claim 20, is wherein mixed in the step in water by this xanthan gum, is water is calorified at the temperature of 36 to 38 DEG C, makes this xanthan gum be easy to dissolve.
The preparation method of 23. bacteria rhodopsin biogels as claimed in claim 20, before wherein this xanthan gum being mixed in to the step in water, be first by this xanthan gum be selected from corn starch, clay and mix with the dry composition of group's combination of oatmeal.
The preparation method of 24. bacteria rhodopsin biogels as claimed in claim 20, before wherein this xanthan gum being mixed in to the step in water, be first by this xanthan gum be selected from glycerol, ethanol and mix with the nonaqueous solvent of group's combination of vegetable oil.
The preparation method of 25. bacteria rhodopsin biogels as claimed in claim 20, wherein this macromolecular compound more comprises carob.
The preparation method of 26. bacteria rhodopsin biogels as claimed in claim 14, wherein this exhaustion culture fluid concentration is 26 to 31% sodium chloride solution.
The preparation method of 27. bacteria rhodopsin biogels as claimed in claim 14, wherein after the step of this cultivation halophilic bacteria, keeps in every liter of bacteria rhodopsin culture fluid, having the living cells of 0.3 to 4 gram.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105456042A (en) * | 2015-12-14 | 2016-04-06 | 诺斯贝尔化妆品股份有限公司 | Compound gel |
| CN108348569A (en) * | 2015-09-03 | 2018-07-31 | 亚历山大·什内拉 | Bacteria rhodopsin as cosmetics and/or pharmaceutical preparation skin disease complex treatment application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6461594B1 (en) * | 1999-01-28 | 2002-10-08 | Syracuse University | Photochromic materials suitable for cosmetic and sunblocking effects |
| CN101254213A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Conditioned medium and its uses |
| CN101254164A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Cosmetic or dermatological composition comprising a cell culture medium |
| CN101268998A (en) * | 2007-03-19 | 2008-09-24 | 胡轩铭 | Active microecological herb amino acid complex and preparation method thereof |
| CN101648010A (en) * | 2008-08-13 | 2010-02-17 | 北京中科颐诺生物技术有限公司 | Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof |
-
2012
- 2012-11-28 CN CN201210495597.2A patent/CN103845276A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6461594B1 (en) * | 1999-01-28 | 2002-10-08 | Syracuse University | Photochromic materials suitable for cosmetic and sunblocking effects |
| CN101254213A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Conditioned medium and its uses |
| CN101254164A (en) * | 2007-02-26 | 2008-09-03 | 莱雅公司 | Cosmetic or dermatological composition comprising a cell culture medium |
| CN101268998A (en) * | 2007-03-19 | 2008-09-24 | 胡轩铭 | Active microecological herb amino acid complex and preparation method thereof |
| CN101648010A (en) * | 2008-08-13 | 2010-02-17 | 北京中科颐诺生物技术有限公司 | Preparation for promoting fibroblasts to secrete extracellular matrix components and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108348569A (en) * | 2015-09-03 | 2018-07-31 | 亚历山大·什内拉 | Bacteria rhodopsin as cosmetics and/or pharmaceutical preparation skin disease complex treatment application |
| CN105456042A (en) * | 2015-12-14 | 2016-04-06 | 诺斯贝尔化妆品股份有限公司 | Compound gel |
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Application publication date: 20140611 |