CN103828837A - Application of novel phosphorus nitrogen mycin A in inhibiting tobacco anthrax germs - Google Patents
Application of novel phosphorus nitrogen mycin A in inhibiting tobacco anthrax germs Download PDFInfo
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- CN103828837A CN103828837A CN201410081348.8A CN201410081348A CN103828837A CN 103828837 A CN103828837 A CN 103828837A CN 201410081348 A CN201410081348 A CN 201410081348A CN 103828837 A CN103828837 A CN 103828837A
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- mycin
- phosphorus nitrogen
- anthracnose
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Abstract
The invention aims at providing application of novel phosphorus nitrogen mycin A in inhibiting tobacco anthrax germs. The molecular formula of the novel phosphorus nitrogen mycin A is C31H51N2O10P, and the molecular weight is 642.72 Dalton. The novel phosphorus nitrogen mycin A is a fermented product of Stfeptomycestauricus. The application has the beneficial effects that the novel phosphorus nitrogen mycin A as a secondary metabolite product of actinomycetes is low in toxicity, high in fermentation yield, easily available in raw materials and high in inhibition on tobacco anthrax germ mycelial growth, and new study and research are developed on applications of preparations aiming at target fungal diseases, in particular tobacco anthracnose.
Description
Technical field
The invention belongs to tobacco pest control techniques field, relate to new phosphorus nitrogen mycin A in the application suppressing in anthracnose of tobacco bacterium.
Background technology
In recent years, from microorganism, isolating biologically active material becomes one of focus of numerous novel pesticide development persons, at present, the antibiotic of extensive use approximately 70% is to come from actinomycetes to produce, have the features such as widely distributed, antimicrobial spectrum is wide, screening technique is various, its research and development have very important scientific value and practical value.New phosphorus nitrogen mycin A comes from the farm antibiotics noval chemical compound of a kind of brand-new anti-crop fungal disease of bull streptomycete (Streptomyces tauricus) tunning, and its low toxicity, to person poultry safety has good DEVELOPMENT PROSPECT.This research invention is on the basis of experiment, adopt mycelial growth rate method, measure new phosphorus nitrogen mycin A relative inhibition to anthracnose of tobacco under different quality concentration, and it has been carried out to the regression analysis of Toxicity Determination, for a new purposes of this antibiotics provides the theoretical foundation that configures novel germicide.
Summary of the invention
The object of the present invention is to provide new phosphorus nitrogen mycin A anthracnose of tobacco bacterium to be produced to the purposes of inhibition.
Feature of the present invention is also that the molecular formula of new phosphorus nitrogen mycin A is C
31h
5ln
2o
l0p, molecular weight is 642.72 dalton Diaton.New phosphorus nitrogen mycin A is bull streptomycete (Streptomyces tauricus) tunning.
New phosphorus nitrogen mycin A is that inventor is in the research work of novel pesticide initiative project, utilize the actinomycetes resource in Yunnan Province, by primary dcreening operation, multiple sieve and the deeply screening of high flux (the sampling point screenings of annual several ten thousand parts), and test, of obtaining has the compound of novel structure.Through TLC, HPLC, IR, UV,
13a large amount of structure elucidation test and studies such as C NMR, 1H NMR, 31P NMR, HLQC and HMBC, the molecular formula of having determined new phosphorus nitrogen mycin A is C
31h
51n
2o
l0p, molecular weight is 642.72.Molecular formula, molecular weight and substituting group and phosphorus azomycin class (Phoslactomycins) compound at C-4, C-8, C-9, C-11, C-19 and C-20 of this compound have obvious difference, belong to the noval chemical compound of the pyranone with phosphate and ammonium salt.In Seiving Tests, we have found that anthracnose of tobacco is had to obvious bactericidal activity first, then carried out the toxicologic study of tobacco to anthrax bacteria, various repeated tests show: new phosphorus nitrogen mycin A is strong to the inhibitory action of anthracnose of tobacco bacterium mycelial growth, the EC the most always obtaining
50for 64.92mg/L, illustrate that anthracnose of tobacco bacterium is strong to the susceptibility of new phosphorus nitrogen mycin A.New phosphorus nitrogen mycin A separates as Yunnan Province the secondary metabolite that the actinomycetes that obtain produce, and raw material is easy to get, and can be used as active substance and kill from now on the core compound of the novel pesticide of anthracnose of tobacco.Therefore need to apply for the protection of this compound for anthracnose of tobacco.At present, the medicament that mostly is chemosynthesis for the method for the control of anthracnose of tobacco bacterium is more, the present invention adopts the new phosphorus nitrogen mycin A of compound one of a kind of brand-new anti-crop fungal disease that comes from bull streptomycete (Streptomyces tauricus) tunning, anthracnose of tobacco bacterium has been carried out to Toxicity Determination, found that new phosphorus nitrogen mycin A is the strongest to the inhibitory action of anthracnose of tobacco bacterium mycelial growth, and anthracnose of tobacco bacterium is stronger to the susceptibility of new phosphorus nitrogen mycin A.
Usefulness of the present invention is that new phosphorus nitrogen mycin A is as actinomycetic secondary metabolite, its toxicity is low, fermentation yield is higher, raw material is easy to get, inhibitory action to anthracnose of tobacco bacterium mycelial growth is strong, can emphasis be the application exploration that main target fungal disease is carried out new invention research and trituration for anthracnose of tobacco.
Embodiment
Application following examples illustrate the application of new phosphorus nitrogen mycin A in inhibition anthracnose of tobacco bacterium:
Embodiment 1: the toxicity test of new phosphorus nitrogen mycin A to anthracnose of tobacco bacterium:
Adopt mycelial growth rate method to measure the Mycelial growth effect of new phosphorus nitrogen mycin A to anthracnose of tobacco bacterium.Taking trial test as basis, the concentration of determining new phosphorus nitrogen mycin A and water is 400,200,100,60, a 20mg/L5 gradient.Take the mother liquor that the former medicine of 0.2g is dissolved in 20mL sterile water and is made into 10000mg/L, then it is made into 5 gradient mass concentrations (40000,20000,10000,6000,2000mg/L) from high to low, compare with sterile distilled water.Get respectively in the triangular flask of PSA medium that 1mL medicament adds the sterilized 100mL of containing, fully shake up, equivalent is poured in the culture dish that 5 diameters are identical respectively, makes band medicine flat board, repeats for three times.Be the dull and stereotyped central authorities of bacterium cake access that 7mm activates in advance and processed with card punch by diameter, mycelia faces down, and is placed in 25 DEG C of incubators and cultivates.Adopt right-angled intersection method to measure pathogen colony diameter and relative inhibition, analyze the virulence size of new phosphorus nitrogen mycin A to pathogen according to the EC50 value of virulence regression equation.Relative inhibition formula is as follows:
Relative inhibition (%)=(contrast bacterium colony average diameter one is processed bacterium colony average diameter)/contrast bacterium colony average diameter × 100.
The measurement result of new phosphorus nitrogen mycin A to anthracnose of tobacco bacterium:
The inhibition difference of the new phosphorus nitrogen mycin A of different quality concentration to anthracnose of tobacco bacterium, under different significances, between each concentration gradient, reached significance level, new phosphorus nitrogen mycin A to the growth inhibition effect of anthracnose of tobacco bacterium in table 1, obvious to the inhibition of pathogen.
Table 1
Note: there were significant differences to be illustrated in 0.05 level with different lowercases after column of figure; Different capitalizations are illustrated in 0.01 level utmost point significant difference.Lower same.
The regression analysis of toxicity test:
The regression analysis of the toxicity test result of new phosphorus nitrogen mycin A to anthracnose of tobacco bacterium is in table 2, regression curve correlation coefficient and the corresponding EC of this former medicine to anthracnose of tobacco bacterium
50value is respectively 0.9906,64.92mg/L; The slope 1.6248 of the regression equation to anthracnose of tobacco bacterium, anthracnose of tobacco bacterium is strong to the susceptibility of new phosphorus nitrogen mycin A.
Table 2
Measurement result: the present invention adopts the new phosphorus nitrogen mycin A of compound one of a kind of brand-new anti-crop fungal disease that comes from bull streptomycete (Streptomyces tauricus) tunning to carry out Toxicity Determination to it, find that new phosphorus nitrogen mycin A is strong to the inhibitory action of anthracnose of tobacco bacterium mycelial growth, for 64.92mg/L, anthracnose of tobacco bacterium is strong to the susceptibility of new phosphorus nitrogen mycin A.New phosphorus nitrogen mycin A is actinomycetes secondary metabolite, and raw material is easy to get, and can be used as active substance and kill from now on the core compound of the novel pesticide of anthracnose of tobacco.
Claims (3)
1. new phosphorus nitrogen mycin A is in the application suppressing in anthracnose of tobacco bacterium.
2., it is characterized in that: the molecular formula of described new phosphorus nitrogen mycin A is C in the application suppressing in anthracnose of tobacco bacterium according to new phosphorus nitrogen mycin A described in claim 1
31h
5ln
2o
10p, molecular weight is 642.72 dalton.
3., it is characterized in that: described new phosphorus nitrogen mycin A is bull streptomycete fermentation product in the application suppressing in anthracnose of tobacco bacterium according to new phosphorus nitrogen mycin A described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410081348.8A CN103828837A (en) | 2014-03-07 | 2014-03-07 | Application of novel phosphorus nitrogen mycin A in inhibiting tobacco anthrax germs |
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| CN201410081348.8A CN103828837A (en) | 2014-03-07 | 2014-03-07 | Application of novel phosphorus nitrogen mycin A in inhibiting tobacco anthrax germs |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107541475A (en) * | 2017-04-25 | 2018-01-05 | 云南中烟工业有限责任公司 | One breeding oxen streptomycete and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720781A (en) * | 2008-10-13 | 2010-06-09 | 云南大学 | New phosphorus and nitrogen mycin A for preventing and controlling fungal disease of crop and preparation process thereof |
-
2014
- 2014-03-07 CN CN201410081348.8A patent/CN103828837A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720781A (en) * | 2008-10-13 | 2010-06-09 | 云南大学 | New phosphorus and nitrogen mycin A for preventing and controlling fungal disease of crop and preparation process thereof |
Non-Patent Citations (1)
| Title |
|---|
| 李铭刚等: "公牛链霉菌EC0 O0008产生的新磷氮霉素A：分离纯化、结构解析及其抗植物病源真菌活性", 《第八届全国新农药创制学术交流会论文集》, 12 March 2010 (2010-03-12), pages 345 - 351 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107541475A (en) * | 2017-04-25 | 2018-01-05 | 云南中烟工业有限责任公司 | One breeding oxen streptomycete and its application |
| CN107541475B (en) * | 2017-04-25 | 2020-06-16 | 云南中烟工业有限责任公司 | Streptomyces bulleyi and application thereof |
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Application publication date: 20140604 |
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