CN103820511B - A kind of method improving xylitol fermentation output - Google Patents
A kind of method improving xylitol fermentation output Download PDFInfo
- Publication number
- CN103820511B CN103820511B CN201410060877.XA CN201410060877A CN103820511B CN 103820511 B CN103820511 B CN 103820511B CN 201410060877 A CN201410060877 A CN 201410060877A CN 103820511 B CN103820511 B CN 103820511B
- Authority
- CN
- China
- Prior art keywords
- fermentation
- xylitol
- sunmorl
- dissolved oxygen
- initial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention provides a kind of method improving xylitol fermentation output, take candida tropicalis as fermentation strain, by adding Sunmorl N 60S, improves xylitol fermentation output.In the inventive method, the Sunmorl N 60S of 5-10g/L is added in initial medium, the flux of phosphopentose pathway in fermenting process can be improved, increase the supply of DPNH I (NADP), make to be that fermenting substrate is produced the transformation efficiency of Xylitol and reached 96.5% with wood sugar, Xylitol output reaches 96.5g/L.The inventive method is simple, and cost is low, less energy consumption, can be used for industrialization scale operation Xylitol.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to a kind of method improving xylitol fermentation output.
Background technology
Xylitol is a kind of important five-state controller, is to extract from other natural plants such as corn cob and through the sweeting agent of chemical process.Estimate according to the insiders, current global Xylitol turnout has reached 5-6 ten thousand tons, and wherein China account for more than 60%, about 3-4 ten thousand tons.And global Xylitol market demand may reach more than 100,000 tons in next three years, potential market demand will up to 40%.But the competition of Xylitol industry is also more and more fierce, controlling cost and ensuring the quality of products is the basic assurance obtaining competitive edge.
Xylose mother liquid is the saturate thick liquid stayed after xylose crystalline, the inside is containing assorted sugar such as wood sugar, L-arabinose, glucose, semi-lactosi, seminoses, wherein the ratio of wood sugar, pectinose, glucose is generally 2:1:1, and other assorted sugar (semi-lactosi seminose) content is below 8%.The total reducing sugar amount that the contains difference of the different manufacturers of xylose mother liquid and different lot number, general total reducing sugar is between 60-75%.Wood sugar accounts between the 50-70% of total reducing sugar.Xylose mother liquid is high due to the assorted sugared content of the inside, causes the solubleness of wood sugar wherein cannot use conventional methods greatly and crystallizes out.If adopt the method for chromatographic separation, sugar different for the inside can be separated.Adopt chromatographic separation technology to be separated various monose, comprise Xylitol, this explained hereafter cost is high.
Current production Xylitol has chemical hydrogenation method and biological fermentation process two kinds.Because xylose mother liquid is containing multiple assorted sugar, chemical hydrogenation method cannot be utilized to produce Xylitol.Production by Microorganism Fermentation Xylitol can avoid violent chemical reaction, and fermentation condition is gentle, does not need special corrosion-resistant anti-high-tension apparatus; The anti-adversity ability of cell can adapt to more extensive raw material; The Substratspezifitaet of biological catalyst ensures to only have Xylitol in product, and purification procedures is simple, significantly can reduce production cost, be considered to the Xylitol production method having development potentiality most.Occurring in nature can utilize wood sugar to produce the microorganism of Xylitol a lot, and from conversion capability, the ability of yeast production Xylitol is the most superior, candida tropicalis (C.tropicalis) especially wherein.
Summary of the invention
The object of this invention is to provide a kind of Sunmorl N 60S that adds in the fermentation medium to improve the method for Xylitol output.The present invention take wood sugar as fermentation substrate, uses candida tropicalis strain, adds Sunmorl N 60S, carried out the production of Xylitol by batch fermentation in fermentation culture.
The party's ratio juris is that gluconic acid can the synthesis of induced glucose acid kinase, gluconic acid is made to synthesize 6-p-gluconic acid under the effect of gluconokinase, 6-p-gluconic acid generates ribose 5-phosphate again under the effect of 6-p-gluconate dehydrogenase, increases the flux of phosphopentose pathway (HMP approach).And the key enzyme Xylose reductase in xylitol fermentation is DPNH I (NADPH) single-minded dependent form, and NADPH is mainly generated in a large number by HMP approach.The enhancing of HMP approach considerably increases the supply of NADPH, thus facilitates the fermentation of Xylitol.
In the present invention, the formula of fermentation culture is: corn steep liquor 3-6g/L, yeast powder 3-6g/L, potassium primary phosphate 6-9g/L, MgSO
47H
2o0.1-0.4g/L, urea 3-6g/L, wood sugar 90-120g/L, Sunmorl N 60S 5-10g/L, prepares with water.
Further, the formula of fermentation culture is: corn steep liquor 3-6g/L, yeast powder 3-6g/L, potassium primary phosphate 6-9g/L, MgSO
47H
2o0.1-0.4g/L, urea 3-6g/L, wood sugar 90-120g/L, Sunmorl N 60S 5-8g/L, prepares with water.
Preferably, the formula of fermentation culture is: corn steep liquor 3.5g/L, yeast powder 3.5g/L, potassium primary phosphate 6g/L, MgSO
47H
2o0.2g/L, urea 3g/L, wood sugar 110g/L, Sunmorl N 60S 6g/L, prepares with water.
Yeast described in the inventive method is the yeast in mycocandida.
Preferably, described yeast is candida tropicalis (C.tropicalis).
In the inventive method, leavening temperature is 30-35 DEG C, initial pH4-5, OD
600nmit is 20%-30%, OD that value controls dissolved oxygen before reaching 20
600nmreduce dissolved oxygen after value reaches 20 to 0%-5%, fermentation period is 60-70 hour.
Preferably, leavening temperature is 33 DEG C, initial pH4-5, OD
600nmit is 20%-30%, OD that value controls dissolved oxygen before reaching 20
600nmreduce dissolved oxygen after value reaches 20 to 0%-5%, fermentation period is 60-65 hour.
The invention provides Sunmorl N 60S and improve the application in xylitol fermentation output.
Above-mentioned application refers to that in fermentation culture, gluconic acid na concn is 5-10g/L.
Particularly, following methods can be adopted to verify, and in real attenuation, Sunmorl N 60S is on the impact of Xylitol output:
Utilize 50L fermentor tank, carry out fermenting experiment using candida tropicalis as fermentation strain, in initial medium, add Sunmorl N 60S, make its final concentration reach 0 respectively, 3,6,9g/L, other nutritive ingredients are identical, are 4-5 at fermentation initial stage NaOH adjust pH, temperature controls as 30-35 DEG C, OD
600nmcontrolling dissolved oxygen by adjustment stirring velocity and air flow before reaching 20 is 20-30%, works as OD
600nmreach after 20, control by reduction stirring velocity and air flow the hypoxia that dissolved oxygen is reduced to 0-5%, and be maintained to fermentation ends, fermentation period is 60-70 hour.
Experimental result shows, does not add Sunmorl N 60S, and when initial Xylose Content is 100g/L, last 64h Determination of Xylitol is only 82.1g/L, and sugar alcohol transformation efficiency is 82.1%.In initial incubation liquid, the Sunmorl N 60S of 6g/L has best effect to xylitol fermentation, makes fermentation period foreshorten to 61 hours, and finally when initial Xylose Content is 100g/L, Xylitol output reaches 96.5g/L, and sugar alcohol transformation efficiency reaches 96.5%.In addition, as shown in Figure 1, the addition (9g/L) increasing Sunmorl N 60S further is not significantly improved to Xylitol output and transformation efficiency, but adds additional production cost.In sum, add the fermentation of 6g/L Sunmorl N 60S to Xylitol and there is best effect.
The method improving Xylitol output by adding Sunmorl N 60S in the fermentation medium provided by the invention, the method is simply effective, because industrial glucose acid sodium consumption is less, to scale operation cost without obvious increase, be therefore applicable to the heavy industrialization fermentative production of Xylitol.
Accompanying drawing explanation
Fig. 1 adds different glucose acid sodium to the effect diagram of xylitol fermentation output.
Embodiment
Following examples further illustrate content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the amendment do the inventive method, step or condition or replacement, all belong to scope of the present invention.
If do not specialize, the conventional means that technique means used in embodiment is well known to those skilled in the art, in embodiment 1-4, adds each raw material unless otherwise indicated, is commercially available.Candida tropicalis (C.tropicalis) deposit number used in the present invention is CICC1316, purchased from Chinese industrial Culture Collection.
Embodiment 1 Sunmorl N 60S addition is 0g/L
In 50L fermentor tank, Preliminary fermentation nutrient solution is settled to 27L, does not add Sunmorl N 60S in nutrient solution.Nutrient solution formula is: corn steep liquor 5g/L, yeast powder 4g/L, potassium primary phosphate 8g/L, MgSO
47H
2o0.2g/L, urea 5g/L, wood sugar 100g/L, prepare with water.
By candida tropicalis kind liquid according to the inoculum size (about 3L) of 10%, access in sterilized initial incubation liquid and ferment.Initial Xylose Content presses 100g/L preparation, and first regulate initial pH value to be 4.5 with NaOH before kind, during the fermentation, leavening temperature maintains 34 DEG C, the not automatic control of fermented liquid pH value.Initial sterile air ventilating ratio is 1:0.6, initial mixing speed is 400rpm, and initial tank pressure is 0.04Mpa, and initial dissolved oxygen is set to 100%, along with the carrying out of fermentation, dissolved oxygen starts to decline, and cell concentration starts continuous increase, when the dense OD of bacterium (600nm) reaches before 20, control dissolved oxygen about 27%, when the dense OD of bacterium (600nm) reaches 20, reduce and be stirred to 150rpm, make dissolved oxygen be reduced to about 3%.Through 64 hours fermentation, Determination of Xylitol reaches 82.1g/L, and wood sugar reaches 82.1% to the mass transitions rate of Xylitol.
Embodiment 2 Sunmorl N 60S addition is 6g/L
In 50L fermentor tank, Preliminary fermentation substratum is settled to 27L, wherein adds Sunmorl N 60S and makes concentration reach 6g/L.Nutrient solution formula is: corn steep liquor 5g/L, yeast powder 4g/L, potassium primary phosphate 8g/L, MgSO
47H
2o0.3g/L, urea 3-6g/L, wood sugar 100g/L, Sunmorl N 60S 6g/L, prepares with water.
By candida tropicalis kind liquid according to the inoculum size (about 3L) of 10%, access in sterilized initial medium and ferment.Initial Xylose Content presses 100g/L preparation, and first regulate initial pH value to be 4.5 with NaOH before kind, during the fermentation, leavening temperature maintains 32 DEG C, the not automatic control of fermented liquid pH value.Initial sterile air ventilating ratio is 1:0.6, initial mixing speed is 400rpm, and initial tank pressure is 0.04Mpa, and initial dissolved oxygen is set to 100%, along with the carrying out of fermentation, dissolved oxygen starts to decline, and cell concentration starts continuous increase, when the dense OD of bacterium (600nm) reaches before 20, control dissolved oxygen about 26%, when the dense OD of bacterium (600nm) reaches 20, reduce and be stirred to 150rpm, make dissolved oxygen be reduced to 2%.Through 60 hours fermentation, Determination of Xylitol reaches 95.1g/L, reaches 95.1% by wood sugar to the mass transitions rate of Xylitol.
Embodiment 3 Sunmorl N 60S addition is 5g/L
In 500L fermentor tank, Preliminary fermentation substratum is settled to 270L, wherein adds Sunmorl N 60S and makes concentration reach 5g/L.The formula of fermentation culture is: corn steep liquor 3g/L, yeast powder 3g/L, potassium primary phosphate 6g/L, MgSO
47H
2o0.1g/L, urea 3g/L, wood sugar 100g/L, Sunmorl N 60S 5g/L, prepares with water.
By candida tropicalis kind liquid according to the inoculum size (about 30L) of 10%, access in sterilized initial medium and ferment.Initial Xylose Content presses 100g/L preparation, and first regulate initial pH value to be 4.5 with NaOH before kind, during the fermentation, leavening temperature maintains 35 DEG C, the not automatic control of fermented liquid pH value.Initial sterile air ventilating ratio is 1:0.4, initial mixing speed is 300rpm, and initial tank pressure is 0.04Mpa, and initial dissolved oxygen is set to 100%, along with the carrying out of fermentation, dissolved oxygen starts to decline, and cell concentration starts continuous increase, when the dense OD of bacterium (600nm) reaches before 20, control dissolved oxygen about 22%, after the dense OD of bacterium (600nm) reaches 20, reduce and be stirred to 100rpm, make dissolved oxygen be reduced to 5%.Through 62 hours fermentation, Determination of Xylitol reaches 94.5g/L, and wood sugar reaches 94.5% to the mass transitions rate of Xylitol.
Embodiment 4 Sunmorl N 60S addition is 9g/L
At 60m
3in fermentor tank, Preliminary fermentation substratum is settled to 27m
3, wherein add Sunmorl N 60S and make concentration reach 9g/L.The formula of fermentation culture is: corn steep liquor 6g/L, yeast powder 6g/L, potassium primary phosphate 9g/L, MgSO
47H
2o0.4g/L, urea 6g/L, wood sugar 100g/L, Sunmorl N 60S 9g/L, prepares with water.
By candida tropicalis kind liquid according to the inoculum size (about 30L) of 10%, access in sterilized initial medium and ferment.Initial Xylose Content presses 100g/L preparation, and first regulate initial pH value to be 4.5 with NaOH before kind, during the fermentation, leavening temperature maintains 33 DEG C, the not automatic control of fermented liquid pH value.Initial sterile air ventilating ratio is 1:0.2, initial mixing speed is 250rpm, and initial tank pressure is 0.02Mpa, and initial dissolved oxygen is set to 100%, along with the carrying out of fermentation, dissolved oxygen starts to decline, and cell concentration starts continuous increase, when the dense OD of bacterium (600nm) reaches before 20, control dissolved oxygen about 20%, when the dense OD of bacterium (600nm) reaches 20, reduce and be stirred to 100rpm, make dissolved oxygen be reduced to about 3%.Through 61.5 hours fermentation, Determination of Xylitol reaches 95.5g/L, and wood sugar reaches 95.5% to the mass transitions rate of Xylitol.
Embodiment 5 Sunmorl N 60S addition is 3g/L
In 500L fermentor tank, Preliminary fermentation substratum is settled to 270L, wherein adds Sunmorl N 60S and makes concentration reach 3g/L.The formula of fermentation culture is: corn steep liquor 5g/L, yeast powder 4g/L, potassium primary phosphate 5g/L, MgSO
47H
2o0.2g/L, urea 4g/L, wood sugar 100g/L, Sunmorl N 60S 3g/L, prepares with water.
By candida tropicalis kind liquid according to the inoculum size (about 30L) of 10%, access in sterilized initial medium and ferment.Initial Xylose Content presses 100g/L preparation, and first regulate initial pH value to be 4.5 with NaOH before kind, during the fermentation, leavening temperature maintains 30 DEG C, the not automatic control of fermented liquid pH value.Initial sterile air ventilating ratio is 1:0.4, initial mixing speed is 300rpm, and initial tank pressure is 0.04Mpa, and initial dissolved oxygen is set to 100%, along with the carrying out of fermentation, dissolved oxygen starts to decline, and cell concentration starts continuous increase, when the dense OD of bacterium (600nm) reaches before 20, control dissolved oxygen about 29%, when the dense OD of bacterium (600nm) reaches 20, reduce and be stirred to 100rpm, make dissolved oxygen be reduced to 0.Through 66 hours fermentation, Determination of Xylitol reaches 86.5g/L, and wood sugar reaches 86.5% to the mass transitions rate of Xylitol.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (5)
1. improve a method for xylitol fermentation output, it is characterized in that, be fermentation substrate with wood sugar, in fermentation culture, add Sunmorl N 60S, produce Xylitol by yeast fermentation;
Wherein, the formula of fermentation culture is: corn steep liquor 3-6g/L, yeast powder 3-6g/L, potassium primary phosphate 6-9g/L, magnesium sulfate heptahydrate 0.1-0.4g/L, urea 3-6g/L, wood sugar 90-120g/L, and Sunmorl N 60S 5-10g/L, prepares with water;
Wherein, described yeast is candida tropicalis (C.tropicalis);
Wherein, leavening temperature is 30-35 DEG C, initial pH4-5, OD
600nmit is 20%-30%, OD that value controls dissolved oxygen before reaching 20
600nmreduce dissolved oxygen after value reaches 20 to 0%-5%, fermentation period is 60-70 hour.
2. the method for claim 1, it is characterized in that, the formula of fermentation culture is: corn steep liquor 3-6g/L, yeast powder 3-6g/L, potassium primary phosphate 6-9g/L, magnesium sulfate heptahydrate 0.1-0.4g/L, urea 3-6g/L, wood sugar 90-120g/L, and Sunmorl N 60S 5-8g/L, prepares with water.
3. the method for claim 1, it is characterized in that, the formula of fermentation culture is: corn steep liquor 3.5g/L, yeast powder 3.5g/L, potassium primary phosphate 6g/L, magnesium sulfate heptahydrate 0.2g/L, urea 3g/L, wood sugar 110g/L, and Sunmorl N 60S 6g/L, prepares with water.
4. the method for claim 1, is characterized in that, leavening temperature is 33 DEG C, initial pH4-5, OD
600nmcontrolling dissolved oxygen before reaching 20 is 20%-30%, OD
600nmreduce dissolved oxygen after value reaches 20 to 0%-5%, fermentation period is 60-65 hour.
5. Sunmorl N 60S is improving the application in xylitol fermentation output,
Wherein, in described fermenting process, be fermentation substrate with wood sugar, in fermentation culture, add Sunmorl N 60S, produce Xylitol by yeast fermentation;
Wherein, the formula of fermentation culture is: corn steep liquor 3-6g/L, yeast powder 3-6g/L, potassium primary phosphate 6-9g/L, magnesium sulfate heptahydrate 0.1-0.4g/L, urea 3-6g/L, wood sugar 90-120g/L, and Sunmorl N 60S 5-10g/L, prepares with water;
Wherein, described yeast is candida tropicalis (C.tropicalis);
Wherein, leavening temperature is 30-35 DEG C, initial pH4-5, OD
600nmit is 20%-30%, OD that value controls dissolved oxygen before reaching 20
600nmreduce dissolved oxygen after value reaches 20 to 0%-5%, fermentation period is 60-70 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060877.XA CN103820511B (en) | 2014-02-21 | 2014-02-21 | A kind of method improving xylitol fermentation output |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410060877.XA CN103820511B (en) | 2014-02-21 | 2014-02-21 | A kind of method improving xylitol fermentation output |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103820511A CN103820511A (en) | 2014-05-28 |
| CN103820511B true CN103820511B (en) | 2016-02-17 |
Family
ID=50755799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410060877.XA Active CN103820511B (en) | 2014-02-21 | 2014-02-21 | A kind of method improving xylitol fermentation output |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103820511B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357339A (en) * | 2014-11-05 | 2015-02-18 | 江南大学 | Strain for producing xylitol and method for producing xylitol |
| CN106148428A (en) * | 2015-03-23 | 2016-11-23 | 河南工业大学 | A kind of immobilized cell multibatches produces the new technique of xylitol |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643753B (en) * | 2009-09-11 | 2012-12-05 | 安徽丰原发酵技术工程研究有限公司 | Preparation method for klinint |
-
2014
- 2014-02-21 CN CN201410060877.XA patent/CN103820511B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103820511A (en) | 2014-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hernández et al. | Saccharification of carbohydrates in microalgal biomass by physical, chemical and enzymatic pre-treatments as a previous step for bioethanol production | |
| Brethauer et al. | Continuous hydrolysis and fermentation for cellulosic ethanol production | |
| Bai et al. | Ethanol fermentation technologies from sugar and starch feedstocks | |
| EP1581617B1 (en) | Methods for producing end-products from carbon substrates | |
| Zhou et al. | Fermentative production of high titer citric acid from corn stover feedstock after dry dilute acid pretreatment and biodetoxification | |
| San-Valero et al. | Influence of electron acceptors on hexanoic acid production by Clostridium kluyveri | |
| US10900016B2 (en) | Method and system for propagating a microorganism | |
| CN101638673B (en) | Method for manufacturing alcohol by utilizing fermentation of plant straws | |
| CN101608192B (en) | Method for producing succinic acid employing corn cob | |
| Shen et al. | Application of low-cost algal nitrogen source feeding in fuel ethanol production using high gravity sweet potato medium | |
| CN103627740B (en) | A kind of microorganism cells conversion method produces the method for 2-KDG | |
| CN103820511B (en) | A kind of method improving xylitol fermentation output | |
| Germec et al. | Kinetic modeling, sensitivity analysis, and techno-economic feasibility of ethanol fermentation from non-sterile carob extract-based media in Saccharomyces cerevisiae biofilm reactor under a repeated-batch fermentation process | |
| CN101880692A (en) | Alcohol fermentation method capable of improving raw material utilization rate | |
| CN103184243A (en) | Fermentation production method for xylitol | |
| EP3041943B1 (en) | A process for microbial fermentation of sugary substrates (wort) by using hydrogen | |
| CN106893683B (en) | Method for expanding culture of saccharomycetes, application of saccharomycetes and method for fermenting ethanol | |
| CN101709309B (en) | Method for combined fermentation of ethanol and xylitol | |
| CN111394397A (en) | Method for producing caproic acid by fermenting kitchen waste | |
| CN104561141A (en) | Acetic fermentation nutrient and use method thereof | |
| AU2014311204A1 (en) | A process for microbial fermentation of sugary substrates and use of the hydrogen in atomic, ionic or gaseous state in said process | |
| CN105624212B (en) | A method of 2,3- butanediol is produced by raw material of microalgae | |
| Gao et al. | An innovative strategy of recycling miscellaneous waste carbohydrates from high-fructose syrup production for Pichia pastoris fermentation | |
| CN105624213B (en) | A method of 2,3- butanediol is produced using microalgae for raw material | |
| CN101643753B (en) | Preparation method for klinint |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |