CN103817322A - PS@Au core-shell structure material, manufacturing method of PS@Au core-shell structure material, and application of PS@Au core-shell structure material - Google Patents
PS@Au core-shell structure material, manufacturing method of PS@Au core-shell structure material, and application of PS@Au core-shell structure material Download PDFInfo
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Abstract
The invention discloses a PS@Au core-shell structure material, a manufacturing method of the PS@Au core-shell structure material, and application of the PS@Au core-shell structure material. The PS@Au core-shell structure material, namely, PS@Au, is composed of a shell and a core. The shell is made of gold, and the core is formed by a PS ball. The shell is connected with the core through polyetherimide with amidogens. A surface active agent with amidogens is connected with the core through a silicon oxygen bond or through physical absorption and connected with the shell through positive and negative electrostatic acting force. The core is located at the center of the shell and surrounded by the shell completely. The PS@Au core-shell structure material is cultured together with cells, and growth of MSC cells can be promoted within a certain concentration range from 0 microgram per milliliter to 90 micrograms per milliliter.
Description
Technical field
The present invention relates to a kind of PS@Au nucleocapsid structure material and preparation method thereof and application.
Background technology
Nano material, due to its unique character, receives much concern as skin effect, small-size effect, quantum size effect, macro quanta tunnel effect etc.Wherein the nanogold particle of various shapes (AuNPs) becomes study hotspot in recent years.Due to its size pattern be easy to control synthetic, chemical stability is high, good biocompatibility, be easy to carry out finishing with and unique photoelectric property, as the surface plasma body resonant vibration of AuNPs (SPR), the character such as SERS (SERS) have a wide range of applications it in biological medicine.AuNPs has report widely at aspects such as bio-imaging technology, bio-sensing, cancer diagnosis, therapy application, medicine transmission.
From the Ancient books of China and Japan, can find that gold can be used as medicine, especially goldleaf.The row of food additives are formally classified goldleaf as in the 16 meeting of the food additive regulations committee of the nineteen eighty-three World Health Organization (FAO/WHO) (CCFA) (Hague, Detch meeting).In recent work more both domestic and external, also propose, nm of gold is at some difficult diseases for the treatment of, as senile dementia (Dingbin Liu et al.A Highly Sensitive Gold-Nanoparticle-Based Assay for Acetylcholinesterase in Cerebrospinal Fluid of Transgenic Mice with Alzheimer's Disease.Advanced Healthcare Materials, 2012, 1, 90 – 95), cosmetic applications (Patel Anuradha et al.OVERVIEW ON APPLICATION OF NANOPARTICLES IN COSMETICS.Asian Journal of Pharmaceutical Sciences and Clinical Research, 2011, Vol.1, Issue2, 40 – 55) and organizational project (Michal Shevach et al.Nanoengineering gold particle composite bers for cardiac tissue engineering.J.Mater.Chem.B, 2013, DOI:10.1039/c3tb20584c) in the middle of, there is important application.The prospect that AuNPs likely becomes future drugs has caused people's interest widely.
In addition,, due to developing rapidly of nano science, excited people to study the huge enthusiasm of various nano particles for organism and cytosis.Wherein about nano particle, the research of the impact of the toxicity on cell and Promote cell's growth has great attraction, has in recent years a large amount of article reports about this work on the one hand.
In the middle of these reports, we can see that gold nano grain has the effect of potential promotion cell proliferation, therefore we can utilize this effect of gold nano grain, realize some and be difficult to fast breeding propagation or benign cell, as liver cell, pancreaticβ-cell, stem cell etc.This is medicinal significant for AuNPs's.Therefore find a kind of method rapidly and efficiently increasing that realizes seed cell on the basis of original cell seed easier, that cost is lower particularly important for organizational project.
Summary of the invention
The object of this invention is to provide a kind of PS@Au nucleocapsid structure material and preparation method thereof and application.
The material with nucleocapsid structure provided by the invention, is also PS Au, is made up of the surfactant of shell, core and band amino;
The material that forms described shell is gold;
The material that forms described core is polystyrene microsphere;
Between described shell and core, be connected by the amino surfactant of described band; Wherein, between the surfactant that described band is amino and core, be connected by silicon oxygen bond or physisorption, and be connected by positive and negative electrostatic force between shell.
In above-mentioned material, the particle diameter of described core is 200nm-3.2 μ m, is specially 250nm, 300nm, 3.2 μ m, 300nm-3.2 μ m or 250-300nm;
The thickness of described shell is 0-30nm, is specially 16nm, 20nm, 25nm, 16-20nm or 20-25nm, and the thickness of described shell is not 0;
The amino surfactant of described band is selected from (3-sulfydryl propyl group) trimethoxy silane, (3-sulfydryl propyl group) triethoxysilane, vinyltrimethoxy silane, octyl group trimethoxy silane, 3-aminopropyl triethoxysilane, 3-aminopropyl trimethoxysilane and N, at least one in N-bis-(amino-ethyl acyl Methylethyl) octadecylamine and PEI;
In the described material with nucleocapsid structure, the mass concentration of gold is 0-90 μ g/ml, is specially 0-50 μ g/ml, is more specifically 10-25 μ g/ml, and is not 0.
Above-mentioned core-shell material be prepare by the following method and.
Preparation provided by the invention has the method for the material of nucleocapsid structure, comprises the steps:
1) surface amination that the aqueous solution stirring of surfactant amino with described band the aqueous solution of aforementioned polystyrene microballoon is carried out to polystyrene microsphere reacted after 20-40 minute, centrifugal collecting precipitation, gained is precipitated to Eddy diffusion in water, add the aqueous solution as the gold nano grain of seed, stirring was reacted after 0.5-1.5 hour, centrifugally remove unnecessary gold nano grain, collecting precipitation obtains PS@AuNP particle;
2) by step 1) gained PS@AuNP particle Eddy diffusion in water, then add growth-promoting media, reducing agent, protective agent and ammoniacal liquor to mix the growth response of carrying out golden shell, react the material described in complete dry obtaining with nucleocapsid structure.
In the step 1) of said method, the concentration of the aqueous solution of polystyrene microsphere is 0-1.5mg/mL, is specially 1mg/mL;
The concentration of the aqueous solution of the amino surfactant of described band is 0-2mg/mL, be specially 1mg/mL, and concentration is not 0;
In above-mentioned surface amination reactions steps, the time is 30 minutes;
The surfactant that described band is amino and the mass ratio of polystyrene microsphere are 0-140:1, are specially 120:1, and quality with amino surfactant is not 0;
In described centrifugal collecting precipitation step, rotating speed is 5000-10000r/min, is specially 5000r/min, 9000r/min, 10000r/min or 5000-9000r/min; Time is 15-20min;
The mass ratio of the described gold nano grain as seed and PS ball is 0-50:1, and the quality of seed gold grain is not 0, is specially 10-30:1; The particle diameter of described gold nano grain is 2-5nm;
Described stirring is carried out in reactions steps, and the time is 1 hour.
In described step 1), be prepare in accordance with the following steps and obtain as the aqueous solution of the gold nano grain of seed:
The HAuCl that is 2% by 125 μ L mass percentage concentration
4the aqueous solution joins 15ml containing in protectant aqueous solution, stirs after 10 minutes, then adds 1ml to contain 0.0013g NaBH
4the aqueous solution carry out reduction reaction after 6 hours, product is dried to after dry powder water-soluble, obtain the described gold nano grain aqueous solution as seed.
Wherein, described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone (K-30); The molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol.
Step 2) in, described growth-promoting media is according to ShiW.; SahooY.; SwihartM.and PrasadP.Gold Nanoshells on Polystyrene Cores for Control of Surface Plasmon Resonance.Langmuir, 2005,21, method that 1610-1617 provides preparation and obtain, the method is specially: by the reactant aqueous solution of the aqueous solution of potash and gold chloride after 30 minutes lucifuge deposit 24 hours and obtain;
Wherein, the concentration of the aqueous solution of described potash is specially 0.25-0.30g/L, is specially 0.27g/L;
The concentration of the aqueous solution of described gold chloride is specially 5mM;
The amount ratio of the aqueous solution of described potash and gold chloride is specially 0.05g:15ml;
Described reducing agent is selected from least one in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride, hydrazine hydrate and formaldehyde;
Described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone; Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol;
The mass percentage concentration of described ammoniacal liquor is 10-35%, is specially 25%;
The volume ratio of described growth-promoting media and polystyrene microsphere is 100-200:1, is specially 100:1;
The volume ratio of the aqueous solution of described growth-promoting media, reducing agent, protectant aqueous solution and ammoniacal liquor is 200:2:2:1;
In the growth response step of described golden shell, temperature is 0-50 ℃, is specially 25 ℃; Time is 8-15 hour, is specially 12 hours.
In addition, the material of what the invention described above provided have nucleocapsid structure promotes the application in people's the product of mescenchymal stem cell propagation in preparation, also belongs to protection scope of the present invention.Wherein, described promotion cell proliferation comprises: have the material of nucleocapsid structure and described people's a mescenchymal stem cell co-incubation in culture medium by described;
In described altogether incubation step, described in there is the concentration of gold element in described culture medium in the material of nucleocapsid structure and be specially 0-90 μ g/ml, be more specifically 0-50 μ g/ml, then be specially 10-25 μ g/ml, and be not 0;
Described culture medium is specially α-MEM culture medium.
The above-mentioned PS@Au nucleocapsid structure material preparing promotes the method for cell proliferation, specifically can comprise the steps: the PS@Au nucleocapsid structure material after freeze drying to be dissolved in culture medium, be configured to certain density solution, after cell 24 hours is adherent, add PS@Au solution and co-culture of cells.
The above-mentioned PS@Au nucleocapsid structure preparing promotes the function of cell proliferation to have concentration dependent, and its concentration is 0-90 μ g/ml, and the larger facilitation of size is better.
The spherical layering Nano composite granules of one that PS@Au core-shell material provided by the invention is made up of golden shell and PS core.The present invention finds nanogold particle to be fixed on while forming PS@Au nucleocapsid structure on the PS ball that diameter is 200nm-3.2 μ m, on the basis that keeps cell primary characteristic, can effectively strengthen the multiplication capacity of mescenchymal stem cell, delay MSC cell ageing, promote the growth of MSC cell.Experimental result shows, the larger promotion proliferation function for MSC cell of size of PS@Au nucleocapsid structure is better, and extends in time facilitation and strengthen.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the PS@Au of preparation in embodiment 1.
Fig. 2 is the stereoscan photograph of the PS@Au of preparation in embodiment 2.
Fig. 3 is the stereoscan photograph of the PS@Au of preparation in embodiment 3.
Fig. 4 is the cytoactive curve after PS@Au nucleocapsid structure and the MSC cytosis of preparing in embodiment 1.
Fig. 5 is the cytoactive curve after PS@Au nucleocapsid structure and the MSC cytosis of preparing in embodiment 2.
Fig. 6 is the cytoactive curve after PS@Au nucleocapsid structure and the MSC cytosis of preparing in embodiment 3.
The specific embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
The material, the reagent etc. that in following embodiment, use, if no special instructions, all can obtain from commercial channels.Following embodiment MSC cell used is purchased from American Type Culture Collection (American Type Culture Collection, ATCC).
In following embodiment, the aqueous solution of the gold nano grain of the seed that is used as is all prepared in accordance with the following steps and obtains:
Get 125 μ L2%HAuCl
4join 15ml containing PVP(K-30) in the aqueous solution of 0.0144g, stir after 10 minutes, add 1ml to contain 0.0013g NaBH
4the aqueous solution, vigorous stirring, reacts and stops for 6 hours, by sample concentration, freeze drying becomes dry powder for subsequent use.The particle diameter of this gold nano grain is 2nm-5nm.
When use, gained dry powder is soluble in water according to proportioning again, as the nm of gold seed solution of preparation PS@Au nucleocapsid structure.
The preparation of the PS@Au nucleocapsid structure material of embodiment 1,250nm
1) prepare PS@AuNP particle:
The aqueous solution of getting 200 μ L particle diameters and be 250nm PS (1mg/ml) joins 24ml PEI (PEI) (1mg/ml) in the aqueous solution, slowly stir 30 minutes, 10000r/min is centrifugal, and unnecessary PEI (PEI) is removed in 20min washing for three times, obtains PS/PEI precipitation.Then PS/PEI precipitation is resuspended in the 3ml aqueous solution, adds the 6ml aqueous solution as the gold nano grain of seed containing 0.006g, slowly stir and centrifugally after 1 hour remove unnecessary gold seeds, obtain PS@AuNP particle;
2) prepare PS@Au nucleocapsid structure material:
Step 1) gained PS@AuNP particle is resuspended in water; obtain 5ml solution; add 20ml growth-promoting media, 200 μ L5% reducing agent Vitamin C aqueous acids; the ammoniacal liquor that the aqueous solution of 200 μ L1% protective agent METHYLPYRROLIDONEs and 100 μ L mass percentage concentration are 25%; in 25 ℃ of growth responses of carrying out shell after 12 hours; the centrifugal 5min washing of 3000r/min three times, freeze drying becomes dry powder, obtains PS@Au nucleocapsid structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 1.As seen from the figure, in this material, the particle diameter of core is 250nm; The thickness of shell is 25nm;
And this material is made up of the surfactant of shell, core and band amino;
The material that forms shell is gold;
The material that forms core is polystyrene microsphere;
Between shell and core, be connected by the surfactant PEI (PEI) with amino; Wherein, with being connected by silicon oxygen bond or physisorption between amino surfactant PEI and core, and be connected by positive and negative electrostatic force between shell.
The preparation of the PS@Au nucleocapsid structure of embodiment 2,300nm.
1) according to the step of embodiment 1, be only that in the aqueous solution of 250nm PS (1mg/ml), the particle diameter of PS replaces with 300nm by particle diameter in step 1), will be wherein " 10000r/min centrifugal 20min washing ", centrifugal rotational speed replaces with 9000r/min, time replaces with 15min, PS@AuNP particle;
2) prepare PS@Au nucleocapsid structure material:
Step 1) gained PS@AuNP particle is resuspended in water, obtain 5ml solution, add 40ml growth-promoting media, 40 μ L formaldehyde (aqueous solution), 400 μ L1%PVP(K30) and the 200 μ L mass percentage concentration ammoniacal liquor that is 25%, 25 ℃ of growth responses of carrying out shell are after 12 hours, the centrifugal 5min washing of 2500r/min three times, freeze drying becomes dry powder, obtains PS@Au nucleocapsid structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 2.As seen from the figure, in this material, the particle diameter of core is 300nm; The thickness of shell is 20nm;
And this material is made up of the surfactant of shell, core and band amino;
The material that forms shell is gold;
The material that forms core is polystyrene microsphere;
Between shell and core, be connected by the surfactant PEI (PEI) with amino; Wherein, with being connected by silicon oxygen bond or physisorption between amino surfactant PEI and core, and be connected by positive and negative electrostatic force between shell.
The preparation of the PS@Au nucleocapsid structure material of embodiment 3,3.2 μ m
1) according to the step of embodiment 1, only by the aqueous solution of 250nm PS (1mg/ml) in step 1), the particle diameter of PS replaces with 3.2 μ m, the consumption of the aqueous solution of PS (1mg/ml) replaces with 800 μ L, and the consumption of PEI (1mg/ml) aqueous solution replaces with 30mL; In " the centrifugal 20min washing of 10000r/min ", centrifugal rotational speed replaces with 5000r/min; The 6ml aqueous solution containing 0.006g seed gold grain replaces with the 6ml aqueous solution containing 0.009g seed gold grain, obtains PS@AuNP particle;
2) prepare PS@Au nucleocapsid structure material:
Step 1) gained PS@AuNP particle is resuspended in water, obtain 5ml solution, add 80ml growth-promoting media, the 800 μ L2% hydroxylamine hydrochloride aqueous solution, the ammoniacal liquor that the 800 μ L1%2-pyrrolidones aqueous solution and 400 μ L mass percentage concentration are 25%, 25 ℃ of growth responses of carrying out shell are after 12 hours, the centrifugal 5min washing of 1500r/min three times, freeze drying becomes dry powder, obtains PS@Au nucleocapsid structure material provided by the invention.
The electromicroscopic photograph of this material as shown in Figure 3.As seen from the figure, in this material, the particle diameter of core is 3.2 μ m; The thickness of shell is 16nm;
And this material is made up of the surfactant of shell, core and band amino;
The material that forms shell is gold;
The material that forms core is polystyrene microsphere;
Between shell and core, be connected by the surfactant PEI (PEI) with amino; Wherein, with being connected by silicon oxygen bond or physisorption between amino surfactant PEI and core, and be connected by positive and negative electrostatic force between shell.
PS@Au nucleocapsid structure material prepared by embodiment 4, embodiment 1 is for promoting MSC cell proliferation
(1) MSC cell kind plate
Vitellophag in the time reaching 90% bottom cell covers blake bottle, digestive juice used is trypsase, the then centrifugal cell suspension that is mixed with 25000/ml, 100 μ L cell suspensions are inoculated in the 96 every holes of orifice plate, and complete inoculation orifice plate is placed in to 37 ℃ containing CO
2in 5% bio-incubator, 24 hour cells are adherent completely rear stand-by.
(2) the PS@Au nucleocapsid structure material dry powder of being prepared by above-described embodiment 1 is dissolved in α-MEM culture medium, is prepared into highly concentrated solution.
Afterwards this concentrated solution is continued, with the dilution of α-MEM culture medium, to be prepared into five containing the dilution of Au mass concentration between 0-50 μ g/ml again, after cell 24 hours is adherent, add 50 these dilutions of μ L, with co-culture of cells.
Acquired results as shown in Figure 4.
As seen from the figure, when the mass concentration of PS@Au surface gold is during at 0-50 μ g/ml, PS@Au nucleocapsid structure has the effect that promotes growth for MSC cell, and when golden mass concentration facilitation maximum during at 25 μ g/ml, and extend in time, facilitation strengthens.
According to upper identical step, the PS@Au nucleocapsid structure material of only being prepared by embodiment 1 replaces with PS@Au nucleocapsid structure material prepared by embodiment 2, gained promote cell proliferation result as shown in Figure 5.
As seen from the figure, when the mass concentration of PS@Au surface gold is during at 0-50 μ g/ml, PS@Au nucleocapsid structure has the effect that promotes growth for MSC cell, and when golden mass concentration facilitation maximum during at 10 μ g/ml, and extend in time, facilitation strengthens.
According to upper identical step, the PS@Au nucleocapsid structure material of only being prepared by embodiment 1 replaces with PS@Au nucleocapsid structure material prepared by embodiment 3, gained promote cell proliferation result as shown in Figure 6.
As seen from the figure, when the mass concentration of PS@Au surface gold is during at 0-40 μ g/ml, PS@Au nucleocapsid structure has the effect that promotes growth for MSC cell, and when golden mass concentration facilitation maximum during at 25 μ g/ml, and extend in time, facilitation strengthens.
Claims (10)
1. there is a material for nucleocapsid structure, formed by the surfactant of shell, core and band amino;
The material that forms described shell is gold;
The material that forms described core is polystyrene microsphere;
Between described shell and core, be connected by the amino surfactant of described band; Wherein, between the surfactant that described band is amino and core, be connected by silicon oxygen bond or physisorption, and be connected by positive and negative electrostatic force between shell.
2. material according to claim 1, is characterized in that: the particle diameter of described core is 200nm-3.2 μ m;
The thickness of described shell is 0-30nm, and the thickness of described shell is not 0;
The amino surfactant of described band is selected from (3-sulfydryl propyl group) trimethoxy silane, (3-sulfydryl propyl group) triethoxysilane, vinyltrimethoxy silane, octyl group trimethoxy silane, 3-aminopropyl triethoxysilane, 3-aminopropyl trimethoxysilane and N, at least one in N-bis-(amino-ethyl acyl Methylethyl) octadecylamine and PEI; Wherein, the molecular weight of described PEI is 10000-30000g/mol, is specially 25000g/mol.
3. according to the arbitrary described material of claim 1-2, it is characterized in that: described in there is nucleocapsid structure material be according to the arbitrary described method preparation of claim 4-8 and obtain.
4. prepare the method that claim 1-2 has the material of nucleocapsid structure described in arbitrary, comprise the steps:
1) the aqueous solution stirring of surfactant amino with described band the aqueous solution of claim 1 or 2 arbitrary described polystyrene microsphere being carried out to surface amination reacted after 20-40 minute, centrifugal collecting precipitation, gained is precipitated to Eddy diffusion in water, add the aqueous solution as the gold nano grain of seed, stirring was reacted after 0.5-1.5 hour, and collecting precipitation obtains PS@AuNP particle;
2) step 1) gained PS@AuNP particle Eddy diffusion is obtained to suspension in water; add again the aqueous solution of growth-promoting media, reducing agent, protectant aqueous solution and ammoniacal liquor to mix the growth response of carrying out golden shell, react the material described in complete dry obtaining with nucleocapsid structure.
5. method according to claim 4, is characterized in that: in described step 1), the concentration of the aqueous solution of polystyrene microsphere is 0-1.5mg/mL, is specially 1mg/mL;
The concentration of the aqueous solution of the amino surfactant of described band is 0-2mg/mL, be specially 1mg/mL, and concentration is not 0;
The surfactant that described band is amino and the mass ratio of polystyrene microsphere are 0-140:1, are specially 120:1, and quality with amino surfactant is not 0;
In described surface amination reactions steps, the time is 30 minutes;
In described centrifugal collecting precipitation step, rotating speed is 5000-10000r/min; Time is 15-20min;
The mass ratio of described gold nano grain and polystyrene microsphere is 0-50:1, and the quality of described gold nano grain is not 0, is specially 10-30:1; The particle diameter of described gold nano grain is 2-5nm;
Described stirring is carried out in reactions steps, and the time is 1 hour.
6. according to the method described in claim 4 or 5, it is characterized in that: described step 2) in, described growth-promoting media be by the reactant aqueous solution of the aqueous solution of potash and gold chloride after 30 minutes lucifuge deposition within 24 hours, obtain;
Wherein, the concentration of the aqueous solution of described potash is specially 0.25-0.30g/L, is specially 0.27g/L;
The concentration of the aqueous solution of described gold chloride is specially 5mM;
The amount ratio of the aqueous solution of described potash and gold chloride is specially 0.05g:15ml;
Described reducing agent is selected from least one in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride, hydrazine hydrate and formaldehyde;
Described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone; Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol;
The mass percentage concentration of described ammoniacal liquor is 10-35%, is specially 25%;
The volume ratio of described growth-promoting media and polystyrene microsphere is 100-200:1, is specially 100:1;
The volume ratio of the aqueous solution of described growth-promoting media, reducing agent, protectant aqueous solution and ammoniacal liquor is 200:2:2:1;
In the growth response step of described golden shell, temperature is 0-50 ℃, is specially 25 ℃; Time is 8-15 hour, is specially 12 hours.
7. according to the arbitrary described method of claim 4-6, it is characterized in that: in described step 1), be to prepare in accordance with the following steps and obtain as the aqueous solution of the gold nano grain of seed:
The HAuCl that is 2% by 125 μ L mass percentage concentration
4the aqueous solution joins 15ml containing in protectant aqueous solution, stirs after 10 minutes, then adds 1ml to contain 0.0013g NaBH
4the aqueous solution carry out reduction reaction after 6 hours, product is dried to after dry powder water-soluble, obtain the described gold nano grain aqueous solution as seed.
8. method according to claim 7, is characterized in that: described protective agent is selected from least one of 2-Pyrrolidone, METHYLPYRROLIDONE, polyethylene glycol, polypropylene glycol and polyvinylpyrrolidone;
Wherein, the molecular weight of described polyethylene glycol is 200-900g/mol; The molecular weight of polypropylene glycol is 400-800g/mol.
9. the material that claim 1-3 has nucleocapsid structure described in arbitrary promotes the application in people's the product of mescenchymal stem cell propagation in preparation.
10. application according to claim 9, is characterized in that: described promotion cell proliferation comprises: have the material of nucleocapsid structure and described people's a mescenchymal stem cell co-incubation in culture medium by described;
In described altogether incubation step, described in there is the concentration of gold element in described culture medium in the material of nucleocapsid structure and be specially 0-90 μ g/ml, be more specifically 0-50 μ g/ml, then be specially 10-25 μ g/ml, and be not 0;
Described culture medium is specially α-MEM culture medium.
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| CN105081345A (en) * | 2015-08-28 | 2015-11-25 | 东北大学 | Preparation method of ultra-thin metal shell layer nano-particles |
| CN106346019A (en) * | 2016-10-26 | 2017-01-25 | 华中科技大学 | Graphene supported micron-gold core-shell structure and preparation method thereof |
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