CN103808712A - Liquid reagent kit applied to acid phosphatase detection and detection method - Google Patents
Liquid reagent kit applied to acid phosphatase detection and detection method Download PDFInfo
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- CN103808712A CN103808712A CN201210436202.1A CN201210436202A CN103808712A CN 103808712 A CN103808712 A CN 103808712A CN 201210436202 A CN201210436202 A CN 201210436202A CN 103808712 A CN103808712 A CN 103808712A
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Abstract
The invention discloses a liquid reagent kit applied to acid phosphatase detection. The liquid reagent kit comprises a matrix solution containing a substrate of acid phosphatase, and a chromogenic solution containing a chromogenic agent which produces chromogenic reaction on a product produced by the reaction of the substrate. The matrix solution and the chromogenic solution are separately stored in the respective suitable pH conditions. When in use, the matrix solution and the chromogenic solution are mixed according to proportion to acquire a work solution. The liquid reagent kit has the advantages of easy use and good stability.
Description
Technical field
The present invention relates to biochemical analysis field.More particularly, the present invention relates to the liquid reagent box of acid phosphatase in detection of biological sample, and apply the method for this kit detection of acidic phosphatase activity.
Background technology
Acid phosphatase (ACP) be a kind of under acid condition catalysis phosphate monoester hydrolysis generate the hydrolytic enzyme of inorganic phosphate, it is present in the multiple human organs such as prostate, liver, spleen and haemocyte, but the abundantest with prostate content.In the time of these histocyte damaged, ACP is released in blood plasma.Therefore, the content of ACP in human body liquid, particularly serum or blood plasma, is common biochemistry detecting item.
At present, the method mainly adopting for detection of the external diagnosis reagent case of ACP is on the market take Alpha-Naphthyl phosphoric acid as substrate, under acid condition, serum ACP catalysis Alpha-Naphthyl phosphoric acid hydrolysis generates alpha-Naphthol and phosphoric acid, alpha-Naphthol reacts with diazo reagent and generates azo-compound, azo-compound has special absorption at 405nm place, calculates the activity of ACP by being determined at the rate of change of 405nm place absorbance.The method adopts continuous detecting method, and operation is relatively simple, convenient, but Alpha-Naphthyl phosphoric acid and diazo reagent poor stability, very easily hydrolysis in the damping fluid of the optimum pH pH5.0 left and right of ACP in reagent causes liquid reagent poor stability.Although most producers adopt the method for freeze-drying to release powdered reagent box, thereby solve the problem of storage of finished products and transportation, but be in use still subject to redissolving the impact of difference between the bottle that the impact of rear stability and the application of sample error of water of redissolving cause.And in use, if packing is too large, just can cause waste later stationary phase after redissolving; If pack too littlely, price own is high, increases chemical examination cost.
Therefore this area need to develop a kind of stable, be convenient to storage with transportation, ACP tracer liquid kit easy to use.
Summary of the invention
One aspect of the present invention provides a kind of liquid reagent box detecting for acid phosphatase, comprise matrix liquid and nitrite ion, matrix liquid and nitrite ion are separately deposited, the substrate that matrix liquid contains acid phosphatase, and nitrite ion contains the developer that the product after described substrate reactions is had to chromogenic reaction.
The present invention also provides the method for activity of acid phosphatase in a kind of detection of biological sample on the other hand, comprises
Provide matrix liquid, the substrate that matrix liquid contains acid phosphatase;
Nitrite ion is provided, and nitrite ion contains the developer that the product after described substrate reactions is had to chromogenic reaction;
Matrix liquid and nitrite ion are mixed to get to working fluid in proportion, and the pH value of described working fluid is at 4-6;
The to be measured biological specimen that contains acid phosphatase is mixed with working fluid, after incubation reaction, detect absorbance;
Calculate the activity of acid phosphatase according to absorbance.
Kit disclosed in this invention, the reagent obtaining after mixing has the character of single reagent, easy to use, and precision of measurement is high.Particularly, have the following advantages:
1, compared with existing dry powder series products, the ACP immue quantitative detection reagent box in the present invention is full liquid reagent, mixed-matrix liquid and nitrite ion before using, redissolution powdered reagent is easy relatively in operation, composition is homogeneous more, and it is poor to reduce between bottle, improves the repeatability that reagent is measured.
2, how many users can prepare appropriate working fluid by a certain percentage according to each specimen amount, joins i.e. use, so just can reduce reagent loss, reduces chemical examination cost.
3, substrate and developer are stored in separately respectively to the most stable pH scope, utilize the difference of pH value, be mixed with in proportion before use working solution, improve the stability of reagent.
Accompanying drawing explanation
What Fig. 1 showed is the range of linearity of the ACP liquid reagent box of embodiment.
Embodiment
Other aspects and advantages of the present invention will become apparent from reading the following description.
definition
Unless otherwise indicated, term used herein has following implication.
Term used herein " substrate ", refers to and can, by the compound of acid phosphatase enzyme hydrolysis, include but not limited to the phosphate compounds such as Alpha-Naphthyl phosphate.
Term used herein " developer ", refer to can with acid phosphatase hydrolysis substrate after the compound of product generation chromogenic reaction, include but not limited to the diazols compounds such as 4-chloro-2-methyl benzene diazotising salt.
Term used herein " biological specimen ", refers to the sample from biosome, includes but not limited to body fluid, serum and plasma.
Term used herein " buffering to ", refers to two class materials of the Conjugate Acid-Base Pairs of composition buffer solution, and they are collectively referred to as buffering to (buffer pair), include but not limited to that citric acid-sodium citrate buffering is right, and acetic acid-sodium acetate buffering is right.
Term used herein " alkyl ", refers to the complete saturated acyclic monoradical of carbon containing and hydrogen, and it can be side chain or straight chain.The example of alkyl is methyl, ethyl, normal-butyl, the tert-butyl group, n-heptyl and isopropyl.Except as otherwise noted, otherwise alkyl described herein is preferably at main chain and contains 1-10 carbon atom and the low alkyl group of 20 carbon atoms at the most, take methyl, ethyl, normal-butyl, isobutyl, the tert-butyl group, n-pentyl and isopentyl as example.
The invention provides the quantitative tracer liquid kit of a kind of ACP, comprise matrix liquid and nitrite ion, matrix liquid and described nitrite ion are separately deposited, the substrate that matrix liquid contains acid phosphatase, and nitrite ion contains the developer that the product after substrate reactions is had to chromogenic reaction.What is called is separately deposited, and is that basidigitale liquid and nitrite ion are spatially separate, can be placed on respectively in two containers, and can be to have in the container of two mutual cavitys.
1) matrix liquid, contains acid phosphatase zymolyte, and this substrate can carry out catalytic reaction with ACP, for example, be hydrolyzed by ACP, is generally phosphate, preferably Alpha-Naphthyl phosphate.
2) nitrite ion, contains developer, for example diazo salt.Substrate, for example Alpha-Naphthyl phosphate, is hydrolyzed by ACP in sample, and the phenol of generation can react with diazo salt and generate coloured azo-compound, and therefore certain wave strong point absorbance changes, and can calculate the activity of ACP by detecting the rate of change of this absorbance.The preferred 4-chloro-2-methyl of described diazo salt benzene diazotising salt (4-chloro-2-methylbenzenediazonium salt, have another name called Fast Red TR Salthemi (zinc chloride) salt), also can adopt other easily and the diazo salt replacement of phenol generation azo-compound.
Preferably, in matrix liquid, also contain damping fluid, matrix liquid is maintained to the stable pH scope of substrate.If substrate is selected Alpha-Naphthyl phosphate, the pH value of matrix liquid can be maintained to 8-11, preferably 10.Can select glycocoll-sodium hydrate buffer solution of pH10.Other damping fluids also can, require as long as can meet pH buffering.If substrate is selected other compound, according to the stable pH scope of this compound solution, the damping fluid that corresponding selection is suitable.
Preferably, nitrite ion also contains damping fluid, and nitrite ion is maintained to the stable pH scope of substrate.If developer is selected the diazo salt of 4-chloro-2-methyl benzene diazotising salt and so on, the pH value of nitrite ion can be maintained and is less than or equal to 3, preferred 1-2, can select glycocoll-hydrochloride buffer of pH1.Other damping fluids also can, require as long as can meet pH buffering.If developer is selected other compound, according to the stable pH scope of this compound solution, the damping fluid that corresponding selection is suitable.
Preferably, in matrix liquid and nitrite ion, containing the pH value that both can be mixed to the working fluid of rear formation, to maintain the buffering of 4-6 right.In measuring, matrix liquid and nitrite ion are mixed to form after working fluid, sample and working fluid mixed determining, and the suitable pH of ACP catalytic hydrolysis reaction is between pH4-6, in matrix liquid and nitrite ion, add respectively a kind of material of pH4-6 buffering centering, so that after matrix liquid and nitrite ion mix, this buffering is to producing suitable surge capability, the pH value of maintenance work liquid.Particularly, if the substrate in matrix liquid is Alpha-Naphthyl phosphate, can in matrix liquid, add the alkaline matter of buffering centering, for example trisodium citrate, sodium acetate or sodium hydrogen phosphate, in nitrite ion, add the acidic materials of buffering centering, for example citric acid, acetic acid.
Preferably, matrix liquid and/or nitrite ion also contain non-ionic surfactant, preferred alkyl phenol polyethenoxy class non-ionic surfactant, and general formula is
r
1be selected from H, C
1-22alkyl or C
2-22alkyl chain thiazolinyl, n is the integer of 5-100.More preferably Triton X-100.The existence of non-ionic surfactant can allow Alpha-Naphthyl phosphate more stable in solution, is conducive to extend the reagent effect phase.Consumption, at 0.01-1%, can be adjusted consumption according to concrete non-ionic surfactant.Can also contain antiseptic, for example Sodium azide (NaN
3), suppress growth of microorganism in solution, extend the reagent effect phase.
Preferably, nitrite ion also contains anionic surfactant, makes diazo salt more stable, preferably has general formula R-SO
3na(II), R is C
8-C
20the alkyl sulfonate of alkyl, more preferably SDS.The consumption of anionic surfactant, at 0.1%-0.01%, can be adjusted consumption according to concrete anionic surfactant.
The method that the invention also discloses activity of acid phosphatase in a kind of detection of biological sample, comprising:
Prepare liquid reagent box as the aforementioned, matrix liquid is mixed in certain proportion with nitrite ion, obtain working fluid, concrete blending ratio is determined according to the concentration that cushions right alkaline matter and acidic materials in these two kinds of solution conventionally, can be according to the pH value fine setting of these two kinds of solution, pH scope 4-6 after guaranteeing to mix, with pH4.8 the best.For example, the matrix liquid of Alpha-Naphthyl phosphoric acid (containing 100mM trisodium citrate, pH10), with the nitrite ion (containing 100mM citric acid, pH1-2) of diazo salt, after mixing, can obtain the working fluid of pH4.8 by 2:1.It will be appreciated by those skilled in the art that substrate in matrix liquid and nitrite ion and the concentration of developer should regulate according to mixing ratio, so that within the concentration range separately of the concentration of the substrate in working fluid and developer in ACP detection method.
By the working fluid preparing and biological specimen, for example contain the serum sample of ACP to be measured, mix, reagent sample volume is than 200:20,37 ℃ of incubation time 5min, analytical approach is KINETIC METHOD, predominant wavelength 412nm, commplementary wave length 700nm, read point time 1-4min, obtain absorbance, go out the activity of ACP according to the absorbance change calculations between the photometry point of specifying.
Embodiment
Below will make further description to the present invention by specific embodiment.Described embodiment only makes exemplary illustration to the present invention, not the present invention is made to the restriction of going up in all senses.
Unless specified otherwise, the chemical reagent that the embodiment of the present invention is used, is analysis pure, purchased from Sigma-aldrich company.Biochemical Analyzer used is to step the BS series automatic clinical chemistry analyzer that auspicious biologic medical electronics incorporated company produces.
Embodiment 1:
By following formulated ACP immue quantitative detection reagent box A, comprise matrix liquid and nitrite ion:
| Matrix liquid | ? |
| Alpha-Naphthyl phosphate | 5.283g |
| Glycocoll | 5.63g |
| Trisodium citrate | 29.41g |
| Triton?X-100 | 1mL |
| Sodium azide | 1g |
| By NaOH adjustment pH value to 10.0 | Constant volume is to 1L |
| Nitrite ion | ? |
| 4-chloro-2-methyl benzene diazotising salt | 5.144g |
| Glycocoll | 5.63g |
| Citric acid | 21.01g |
| Triton?X-100 | 1mL |
| Lauryl sodium sulfate (SDS) | 0.5g |
| By HCl adjustment pH value to 1.2 | Constant volume is to 1L |
Matrix liquid is mixed and obtains working fluid with 2:1 with nitrite ion, and working fluid pH value is 4.8.
Set automatic clinical chemistry analyzer parameter: analytical approach is KINETIC METHOD, predominant wavelength 412nm, commplementary wave length 700nm, reagent sample volume is than 200:20, incubation time 5min, read point time 1-4min, the U/L of result unit, 37 ℃ of reaction cup temperature.Use ACP calibration object to calibrate, after calibration, detecting instrument can calculate pattern detection result automatically.
Get two parts of fresh serums (a ACP normal level concentration, a ACP abnormal level concentration), by upper sample measuring method repeated test 20 times, calculate the mean value of 20 measured values with above-mentioned kit A
and standard deviation (s).Press formula
calculate the coefficient of variation (CV), be batch interior repeatability.Result of calculation is low value sample CV ﹤ 2%, and high value sample CV ﹤ 0.5%, meets the requirements.The results are shown in Table 1.
Separately get the check reagent for the treatment of of 3 batches, test respectively ACP normal level concentration and the fresh patients serum of ACP abnormal level concentration, each lot number test 3 times, calculates respectively every batch of average of measuring for 3 times at each concentration level
(i=1,2,3), calculate relative deviation (CV) by formula below, are difference between batch.
In formula:
in maximal value;
Result of calculation is low value sample CV ﹤ 1%, and high value sample CV ﹤ 1%, meets the requirements, and the results are shown in Table 1.。Above result shows, in batch and batch between repeatability better, show to use this kit to detect the active reliable results of serum ACP, repeatability is high.
Table 1 liquid A CP kit criticize interior and batch between reperformance test
Embodiment 2:
Take appropriate amount of acid acid phosphatase dry powder sterling, with distilled water dilution, preparation ACP activity is respectively the titer of 30U/L, 60U/L, 90U/L, 120U/L, uses the every sample repeated test of mentioned reagent box A 3 times, calculates its mean value (y).Concrete method of testing is with embodiment 1.
Take concentration of standard solution as horizontal ordinate (x), measurement result average is that ordinate (y) is done linear regression, calculate linear regression correlation coefficient r, require linear regression correlation coefficient r >=0.99, simultaneously (x) substitution equation of linear regression of concentration of standard solution, calculate linear regression value (y1), calculate relative deviation or the absolute deviation (S) of each mensuration mean value (y) and linear regression value (y1) according to following formula.
Absolute deviation=(y-y1)
Relative deviation S=(y-y1)/y1 × 100%
Concrete outcome is shown in Fig. 1, and ACP activity linear relationship between 0-100U/L is good as can be seen from the results, R
2=0.9986, each concentration level S value is all less than 5%.It is wide that above result shows that this kit detects the active range of linearity of serum ACP, excellent performance.
Embodiment 3:
Get the above-mentioned kit A preparing some, be stored in respectively 4 ℃ and room temperature (25 ℃), regularly opening encapsulation reagent carries out outward appearance and measures and range of linearity test with proterties, quality-control product deviation, investigate 4 ℃ with room temperature under the stability of reagent, the results are shown in Table 2.
Concrete method of testing is with embodiment 1.
Result shows, reagent room temperature preservation the 0th, 1, February outward appearance do not change, be colourless transparent liquid, pH value is constant; Room temperature preservation the 0th, 1, February measure Quality Control deviation and are all less than 10%; Room temperature preservation the 60th day, range of linearity 0-100U/L.Be stored under 4 ℃ of environment, the the the the 0th, 2,4,6,8,10, Dec measure, outward appearance is colourless transparent liquid, pH value is substantially unchanged; Quality Control deviation is all less than 10%; Preserve 12 months range of linearity 0-100U/L for 4 ℃.Show that liquid A CP kit is more stable under 4 ℃ of environment, the term of validity is for being no less than 1 year.
The test of table 2 liquid A CP reagent stability
Embodiment 4:
By following formulated ACP immue quantitative detection reagent box B, comprise matrix liquid and nitrite ion:
| Matrix liquid | ? |
| Alpha-Naphthyl phosphoric acid | 5.283g |
| Glycocoll | 5.63g |
| Trisodium citrate | 29.41g |
| Sodium azide | 1g |
| By NaOH adjustment pH value to 10.0 | Constant volume is to 1L |
| Nitrite ion | ? |
| 4-chloro-2-methyl benzene diazotising salt | 5.144g |
| Glycocoll | 5.63g |
| Citric acid | 21.01g |
| By HCl adjustment pH value to 1.2 | Constant volume is to 1L |
Matrix liquid is mixed and obtains working fluid with 2:1 with nitrite ion, and working fluid pH value is 4.8.
Embodiment 5
By following formulated ACP immue quantitative detection reagent box C, comprise matrix liquid and nitrite ion:
| Matrix liquid | ? |
| Alpha-Naphthyl phosphoric acid | 5.283g |
| Glycocoll | 5.63g |
| Trisodium citrate | 29.41g |
| Triton?X-100 | 1mL |
| Sodium azide | 1g |
| By NaOH adjustment pH value to 10.0 | Constant volume is to 1L |
| Nitrite ion | ? |
| 4-chloro-2-methyl benzene diazotising salt | 5.144g |
| Glycocoll | 5.63g |
| Citric acid | 21.01g |
| Triton?X-100 | 1mL |
| By HCl adjustment pH value to 1.2 | Constant volume is to 1L |
Matrix liquid is mixed and obtains working fluid with 2:1 with nitrite ion, and working fluid pH value is 4.8.
Embodiment 6
Get mentioned reagent box A, B, C are some, be stored in respectively 4 ℃ and 37 ℃, regularly opening encapsulation reagent carries out quality-control product test bias, detect same quality-control product with refrigeration reagent and acceleration reagent simultaneously, measure average as reference value (y) to refrigerate the sample of reagent, measure average as measured value (y1) to accelerate the sample of reagent, calculate the sample mensuration relative deviation that accelerates reagent according to following formula, with the stability of more several different reagent, the less reagent of deviation is more stable, the results are shown in Table 3.Concrete method of testing is with embodiment 1.
Relative deviation S=(y-y1)/y1 × 100%
Result shows, compared with not adding the kit B of any surfactant, add the kit C stability of non-ionic surfactant better, adding in addition the kit A stability of anionic surfactant is three's optimum, illustrates and is of value to the stable of reagent adding of non-ionic surfactant and anionic surfactant.
The test of table 3 liquid A CP reagent stability
Embodiment 7
By following formulated ACP immue quantitative detection reagent box D, comprise matrix liquid and nitrite ion:
| Matrix liquid | ? |
| Alpha-Naphthyl phosphate | 5.283g |
| Glycocoll | 5.63g |
| Sodium acetate | 24.09g |
| Triton?X-100 | 0.1mL |
| Sodium azide | 1g |
| By NaOH adjustment pH value to 8.0 | Constant volume is to 1L |
| Nitrite ion | ? |
| 4-chloro-2-methyl benzene diazotising salt | 5.144g |
| Glycocoll | 5.63g |
| Acetic acid | 41mL |
| Triton?X-100 | 0.1mL |
| Lauryl sodium sulfate (SDS) | 0.1g |
| By HCl adjustment pH value to 3.0 | Constant volume is to 1L |
Matrix liquid is mixed and obtains working fluid with 2:1 with nitrite ion, and working fluid pH value is 4.7.
Embodiment 8
By following formulated ACP immue quantitative detection reagent box E, comprise matrix liquid and nitrite ion:
| Matrix liquid | ? |
| Alpha-Naphthyl phosphate | 5.283g |
| Glycocoll | 5.63g |
| Sodium hydrogen phosphate | 20.99g |
| Triton?X-100 | 10mL |
| Sodium azide | 1g |
| By NaOH adjustment pH value to 11.0 | Constant volume is to 1L |
| Nitrite ion | ? |
| 4-chloro-2-methyl benzene diazotising salt | 5.144g |
| Glycocoll | 5.63g |
| Citric acid | 31.96g |
| Triton?X-100 | 10mL |
| Lauryl sodium sulfate (SDS) | 1g |
| By HCl adjustment pH value to 1.0 | Constant volume is to 1L |
Matrix liquid is mixed and obtains working fluid with 2:1 with nitrite ion, and working fluid pH value is 5.0.
Embodiment 9
Get mentioned reagent box A, D, E are some, be stored in respectively 4 ℃ and 37 ℃, regularly opening encapsulation reagent carries out quality-control product test bias, detect same quality-control product with refrigeration reagent and acceleration reagent simultaneously, press the method for embodiment 6 and calculate the sample mensuration deviation of refrigeration reagent relatively of accelerating reagent, the less reagent of deviation is more stable, the results are shown in Table 4.Concrete method of testing is with embodiment 1, and computing method are with embodiment 6.Result shows, ACP reagent colour development liquid and matrix liquid are all better at suitable pH scope internal stability.
The test of the different pH liquid A of table 4 CP reagent stability
From above embodiment, acid phosphatase tracer liquid kit of the present invention, is separately formulated as matrix liquid and nitrite ion by substrate and developer, preserves separately.When use, the working fluid that is mixed in proportion pH4-6 is measured, and has good precision, detects linearity and stability, and commercial application value is high.In matrix liquid and nitrite ion, add non-ionic surfactant, in nitrite ion, add anionic surfactant, can improve the stability of reagent.
All data, image, instrument, reagent and step is herein interpreted as illustrative and is nonrestrictive.Although described the present invention in conjunction with above-mentioned specific embodiments, many modifications and other variation are apparent for those skilled in the art.All this modifications and other variation also fall in the spirit and scope of the present invention.
Claims (12)
1. the liquid reagent box detecting for acid phosphatase, comprise matrix liquid and nitrite ion, described matrix liquid and described nitrite ion are separately deposited, the substrate that described matrix liquid contains acid phosphatase, and described nitrite ion contains the developer that the product after described substrate reactions is had to chromogenic reaction.
2. liquid reagent box according to claim 1, is characterized in that: described substrate is selected from phosphate, preferably Alpha-Naphthyl phosphate.
3. liquid reagent box according to claim 1, is characterized in that: described developer is selected from diazo salt, preferably 4-chloro-2-methyl benzene diazotising salt.
4. liquid reagent box according to claim 1, is characterized in that: the damping fluid that described matrix liquid contains pH value 8-11, the damping fluid that preferably pH value is 10.
5. liquid reagent box according to claim 1, is characterized in that: described nitrite ion contains the damping fluid that pH value is less than 3, preferably the damping fluid of pH value 1-2.
6. liquid reagent box according to claim 1, it is characterized in that: described matrix liquid and described nitrite ion obtain working fluid after mixing, in described matrix liquid, also contain the class in the two class materials that buffering is right, described nitrite ion also contains another kind of in the two class materials that described buffering is right, and described buffering is to maintaining pH4-6 by the pH value of described working fluid.
7. liquid reagent box according to claim 6, is characterized in that: in described matrix liquid, contain the alkaline matter in the two class materials that described buffering is right, described nitrite ion contains the acidic materials in the two class materials that described buffering is right.
8. liquid reagent box according to claim 1, is characterized in that: described matrix liquid and/or nitrite ion also contain non-ionic surfactant, and preferred described non-ionic surfactant has the structure of general formula I
R
1be selected from H, C
1-22alkyl or C
2-22alkyl chain thiazolinyl, n is the integer of 5-100.
9. liquid reagent box according to claim 1, is characterized in that: described nitrite ion also contains anionic surfactant, preferred described anionic surfactant has the structure of general formula I I
R-SO
3Na（II）
R is C
8-C
20alkyl.
10. a method for activity of acid phosphatase in detection of biological sample, comprises
Provide matrix liquid, the substrate that described matrix liquid contains acid phosphatase;
Nitrite ion is provided, and described nitrite ion contains the developer that the product after described substrate reactions is had to chromogenic reaction;
Described matrix liquid and described nitrite ion are mixed to get to working fluid in proportion, and the pH value of described working fluid is at 4-6;
The to be measured biological specimen that contains acid phosphatase is mixed with described working fluid, after incubation reaction, detect absorbance;
Calculate the activity of described acid phosphatase according to described absorbance.
11. methods according to claim 10, is characterized in that: the substrate of described matrix liquid is Alpha-Naphthyl phosphoric acid, and the pH value of described matrix liquid is 8-11.
12. methods according to claim 10, is characterized in that: the developer of described nitrite ion is diazo salt, and the pH value of described nitrite ion is less than 3.
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| CN109576343A (en) * | 2018-12-29 | 2019-04-05 | 重庆博蓝鹰生物技术有限公司 | A kind of formula of continuity method measurement activity of acid phosphatase kit |
| CN112326639A (en) * | 2020-11-25 | 2021-02-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
| CN114457141A (en) * | 2022-02-16 | 2022-05-10 | 北京九强生物技术股份有限公司 | Quantitative detection kit for prostatic acid phosphatase |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109576343A (en) * | 2018-12-29 | 2019-04-05 | 重庆博蓝鹰生物技术有限公司 | A kind of formula of continuity method measurement activity of acid phosphatase kit |
| CN112326639A (en) * | 2020-11-25 | 2021-02-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
| CN112326639B (en) * | 2020-11-25 | 2024-01-05 | 迈克生物股份有限公司 | Kit and method for detecting fructosamine |
| CN114457141A (en) * | 2022-02-16 | 2022-05-10 | 北京九强生物技术股份有限公司 | Quantitative detection kit for prostatic acid phosphatase |
| CN114457141B (en) * | 2022-02-16 | 2024-05-24 | 北京九强生物技术股份有限公司 | Quantitative detection kit for prostatic acid phosphatase |
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| CN103808712B (en) | 2017-12-19 |
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Application publication date: 20140521 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: A liquid kit and detection method for acid phosphatase detection Granted publication date: 20171219 License type: Common License Record date: 20220804 |