CN103806115B - A kind of luminous nano fibre with biocompatibility and preparation method thereof - Google Patents
A kind of luminous nano fibre with biocompatibility and preparation method thereof Download PDFInfo
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- CN103806115B CN103806115B CN201210456323.2A CN201210456323A CN103806115B CN 103806115 B CN103806115 B CN 103806115B CN 201210456323 A CN201210456323 A CN 201210456323A CN 103806115 B CN103806115 B CN 103806115B
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Abstract
The present invention relates to a kind of preparation method with the luminous nano fibre of biocompatibility, carbon nano-particles is dissolved in solvent, under agitation, add macromolecular material, make itself and carbon nano-particles mass ratio 0.5:1 ~ 1:2, stir 1 ~ 3 hour, finally obtain uniform spinning solution; Above-mentioned spinning solution is poured into syringe, regulates electrostatic spinning process, the uniform white fiber of one deck collected by reception aluminium foil; Above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, passes into nitrogen, be warming up to 600 ~ 1000 DEG C, be incubated 1 ~ 5 hour, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.Obtained fiber had both had good biocompatibility, had both luminescent properties again simultaneously, was more suitable for biomedical sector, as a kind of timbering material, can be convenient to later observations especially.Preparation technology of the present invention is simple, and production cost is low, can meet the demand of producing and applying further.
Description
Technical field
The present invention relates to a kind of nanofiber and preparation method thereof, be specifically related to excellent luminous nano fibre of a kind of biocompatibility and preparation method thereof.This method belongs to the technology of preparing of fluorescent nano-fiber, and prepared material can be widely used in biology, medicine and other fields.
Background technology
Electrostatic spinning technique is one and prepares the simple and effective technology of nanometer stage material.Polymer melt or solution, under high-pressure electrostatic effect, utilize electric field force to overcome polymer solution surface tension and form one charged injection stream, and solvent evaporates solidification afterwards, nano-scale fiber is arranged on collecting board disorderly, forms similar non-woven fabrics fiber film.Electrostatic spinning technique has been advantageously applied to prepares silk fibroin nano-fiber film, and prepared tunica fibrosa has fibre number is thin, surface area is large, porosity is high Morphological Features and can be used as the porous support of Growth of Cells, promotes migration and the propagation of cell; Also can be used as artificial graft's blood vessel, surface of a wound cladding material and pharmaceutical carrier, be widely used as organizational project and bio-medical engineering material.
In recent years, prepared many functional nano fibers by electrostatic spinning technique, made it be applied to technical field of biological material better.Up to the present, prepared as antibacterial nano fiber, functional fibre such as hemostasis nanofiber, fluorescent nano-fiber etc.Wherein, Chinese scholars prepares fluorescent nano-fiber by electrostatic spinning technique, has achieved some achievements in research.In addition, there was reported multi-functional magnetic ~ fluorescent nano-fiber.But these are semi-conducting material, rare earth material etc. for the fluorescent nano material of filling, and these materials do not possess good biological safety, and therefore these fluorescent nano-fibers are not suitable for biological field.
In recent years, there is a kind of novel fluorescent carbon nano material---fluorescent carbon nano-particles, there is abundant photoluminescent property, and compare with quantum dot, up-conversion luminescence nano particle, fluorescent carbon nano-particles is except good stability, luminous intensity advantages of higher, surface has abundant oxy radical, easy functionalization.The most important thing is to have low toxicity characteristic, scientist extracts carbon nanometer from copolymerization sugar, in zooblast, then check the toxicity of these nano particles, found that, even if under very high concentration, these nano particles also only have very little toxicity, or even nontoxic.Thus, develop carbon nano-particles, in the application of biomedicine field, there is important practical significance.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of luminous nano fibre with biocompatibility and preparation method thereof.Carbon nano-particles is scattered in solvent by the method, with macromolecular material compound, is mixed to form spinning solution, then prepares the excellent luminous nano fibre of biocompatibility by electrostatic spinning technique.
There is a preparation method for the luminous nano fibre of biocompatibility, it is characterized in that, comprise the following steps:
1) carbon nano-particles is dissolved in solvent, ultrasound condition 0.5 ~ 4 hour, makes its mass concentration be 0.01 ~ 0.5 grams per milliliter; Under agitation, add macromolecular material, make itself and carbon nano-particles mass ratio 0.5:1 ~ 1:2, stir 1 ~ 3 hour, finally obtain uniform spinning solution;
2) above-mentioned spinning solution is poured into syringe, regulate electrostatic spinning process, the uniform white fiber of one deck collected by reception aluminium foil;
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 600 ~ 1000 DEG C, be incubated 1 ~ 5 hour, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.
The particle diameter of described carbon nano-particles is 50 ~ 300 nanometers.
Described solvent is one in water, ethanol, chloroform, N, N ~ dimethyl formamide, dimethyl sulfoxide (DMSO), ether, hexafluoroisopropanol, formic acid or its combination.
Described macromolecular material is the one in carboxymethyl cellulose, polyvinylpyrrolidone, chitin, shitosan, PLA, polyglycolic acid, polycaprolactone, poly-hydroxy fatty acid, fibroin albumen, collagen.
Described electrostatic spinning process is voltage 10 ~ 20 kilovolts, liquid inventory 0.1 ~ 0.5 ml/hour, and receiving range is 10 ~ 20 centimetres.
A kind of luminous nano fibre with biocompatibility is prepared by said method.
The invention has the advantages that: by electrostatic spinning technique, prepare nano level composite fibre.This fiber had both had good biocompatibility, had both luminescent properties again simultaneously, was more suitable for biomedical sector, as a kind of timbering material, can be convenient to later observations especially.Preparation technology of the present invention is simple, and production cost is low, can meet the demand of producing and applying further.
Accompanying drawing explanation
The stereoscan photograph of the nano fibrous membrane of Fig. 1 prepared by embodiment 1.
The stereoscan photograph of the nano fibrous membrane of Fig. 2 prepared by embodiment 2.
The emission spectrum of the nano fibrous membrane of Fig. 3 prepared by embodiment 3.
The emission spectrum of the nano fibrous membrane of Fig. 4 prepared by embodiment 4.
Detailed description of the invention
Below by way of specific embodiment, technical scheme of the present invention is further described.Following embodiment further illustrates of the present invention, and do not limit the scope of the invention.
Embodiment 1:
1) by 0.5 gram, the carbon nano-particles of 100 nanometers is dissolved in 10 ml waters, ultrasound condition 0.5 hour, makes its mass concentration be 0.05 grams per milliliter.Under agitation, add 1 gram of polyvinylpyrrolidone, stir 1 hour, finally obtain uniform spinning solution.
2) above-mentioned spinning solution is poured into syringe, regulation voltage 10 kilovolts, liquid inventory 0.1 ml/hour, receiving range is 10 centimetres, and the uniform white fiber of one deck collected by reception aluminium foil.
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 600 DEG C, be incubated 3 hours, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.
Fig. 1 is the stereoscan photograph of prepared nano fibrous membrane.As seen from the figure, the diameter of nanofiber is 500 nanometers.
Embodiment 2:
1) by 1 gram, the carbon nano-particles of 300 nanometers is dissolved in the ethanol/chloroform of 10 milliliters of 1:1, ultrasound condition 1 hour, makes its mass concentration be 0.1 grams per milliliter.Under agitation, add 0.5 gram of PLA, stir 2 hours, finally obtain uniform spinning solution.
2) above-mentioned spinning solution is poured into syringe, regulation voltage 15 kilovolts, liquid inventory 0.1 ml/hour, receiving range is 10 centimetres, and the uniform white fiber of one deck collected by reception aluminium foil.
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 800 DEG C, be incubated 2 hours, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.
Fig. 2 is the stereoscan photograph of prepared nano fibrous membrane.As seen from the figure, the diameter of nanofiber is 1000 nanometers.
Embodiment 3:
1) by 0.1 gram, the carbon nano-particles of 200 nanometers is dissolved in 10 milliliters of ethanol, ultrasound condition 1 hour, makes its mass concentration be 0.01 grams per milliliter.Under agitation, add 0.2 gram of shitosan, stir 3 hours, finally obtain uniform spinning solution.
2) above-mentioned spinning solution is poured into syringe, regulation voltage 20 kilovolts, liquid inventory 0.2 ml/hour, receiving range is 15 centimetres, and the uniform white fiber of one deck collected by reception aluminium foil.
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 600 DEG C, be incubated 2 hours, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.
Fig. 3 is take 800nm as the emission spectrum of excitation wavelength, nano fibrous membrane.As seen from the figure, the emission wavelength of nano fibrous membrane is 530nm, and intensity is 1040.
Embodiment 4:
1) by 0.2 gram, the carbon nano-particles of 50 nanometers is dissolved in 10 ml waters, ultrasound condition 2 hours, makes its mass concentration be 0.02 grams per milliliter.Under agitation, add 0.2 gram of fibroin albumen, stir 3 hours, finally obtain uniform spinning solution.
2) above-mentioned spinning solution is poured into syringe, regulation voltage 15 kilovolts, liquid inventory 0.2 ml/hour, receiving range is 10 centimetres, and the uniform white fiber of one deck collected by reception aluminium foil.
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 600 DEG C, be incubated 1 hour, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles.
Fig. 4 is take 800nm as the emission spectrum of excitation wavelength, nano fibrous membrane.As seen from the figure, the emission wavelength of nano fibrous membrane is 540nm, and intensity is 105.
Claims (2)
1. there is a preparation method for the luminous nano fibre of biocompatibility, it is characterized in that, comprise the following steps:
1) carbon nano-particles is dissolved in solvent, ultrasound condition 0.5 ~ 4 hour, makes its mass concentration be 0.01 ~ 0.5 grams per milliliter; Under agitation, add macromolecular material, make itself and carbon nano-particles mass ratio 1:2, stir 1 ~ 3 hour, finally obtain uniform spinning solution;
2) above-mentioned spinning solution is poured into syringe, regulate electrostatic spinning process, the uniform white fiber of one deck collected by reception aluminium foil;
3) above-mentioned white fiber is put into high-temperature tubular atmosphere furnace, pass into nitrogen, be warming up to 600 ~ 1000 DEG C, be incubated 1 ~ 5 hour, be finally cooled to room temperature, obtain the luminous nano fibre containing carbon nano-particles;
The particle diameter of described carbon nano-particles is 50 ~ 300 nanometers;
Described solvent is one in water, ethanol, chloroform, N, N ~ dimethyl formamide, dimethyl sulfoxide (DMSO), ether, hexafluoroisopropanol, formic acid or its combination;
Described macromolecular material is the one in carboxymethyl cellulose, polyvinylpyrrolidone, chitin, shitosan, PLA, polyglycolic acid, polycaprolactone, poly-hydroxy fatty acid, fibroin albumen, collagen;
Described electrostatic spinning process is voltage 10 ~ 20 kilovolts, liquid inventory 0.1 ~ 0.5 ml/hour, and receiving range is 10 ~ 20 centimetres.
2. have a luminous nano fibre for biocompatibility, it is characterized in that, method according to claim 1 prepares.
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| CN106947466B (en) * | 2017-03-30 | 2019-11-15 | 延边大学 | Carbon dots-porous inorganic oxide composite nano fiber preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876902A (en) * | 2006-07-10 | 2006-12-13 | 东华大学 | Atmosphere controllable static spinning device and industrial application thereof |
| CN101244902A (en) * | 2007-06-15 | 2008-08-20 | 东华大学 | Fluorescence fibre for reinforcing concrete, production and application thereof |
| JP2010236117A (en) * | 2009-03-31 | 2010-10-21 | Unitika Ltd | Luminescent fibrous structural material |
| CN101962818A (en) * | 2010-09-08 | 2011-02-02 | 黑龙江大学 | Preparation method of doping type fluorescent micron-nano fibers |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1876902A (en) * | 2006-07-10 | 2006-12-13 | 东华大学 | Atmosphere controllable static spinning device and industrial application thereof |
| CN101244902A (en) * | 2007-06-15 | 2008-08-20 | 东华大学 | Fluorescence fibre for reinforcing concrete, production and application thereof |
| JP2010236117A (en) * | 2009-03-31 | 2010-10-21 | Unitika Ltd | Luminescent fibrous structural material |
| CN101962818A (en) * | 2010-09-08 | 2011-02-02 | 黑龙江大学 | Preparation method of doping type fluorescent micron-nano fibers |
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