CN103805654A - Method for producing isomalto-oligosaccharide - Google Patents
Method for producing isomalto-oligosaccharide Download PDFInfo
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- CN103805654A CN103805654A CN201210452471.7A CN201210452471A CN103805654A CN 103805654 A CN103805654 A CN 103805654A CN 201210452471 A CN201210452471 A CN 201210452471A CN 103805654 A CN103805654 A CN 103805654A
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- oligomeric isomaltose
- transglucosidase
- production
- malt syrup
- saccharification
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Abstract
The invention discloses a method for continuously producing isomalto-oligosaccharide by using an ultrafiltration membrane. The method comprises the following steps: by taking maltose syrup as a raw material, performing raw material sterilization, saccharifying glycoside by a saccharification kettle, continuously recovering transglycosylase by the ultrafiltration membrane, performing ion exchange and vacuum concentration, and forming a product. The method has the advantages that 1, the saccharification glycoside reaction is continuously carried out, the production efficiency is high, and the required saccharification kettle is small in number in comparison with the traditional intermittent saccharification glycosides process; 2, a cross-flow filtration technology is adopted to filter by the ultrafiltration membrane, the transglycosylase can be directly returned to the saccharification kettle, can be recycled, and is high in unit enzyme production rate and low in production cost; 3, a reaction substrate is high in concentration, and low in energy consumption for a later evaporation procedure; 4, the traditional decoloring technology is not needed, activated carbon is saved, and the pollution to the environment is reduced; 5, the continuous production technology is stable, and easy to control, and the product quality can be effectively ensured; and 6, equipment is high in automatic degree, and low in labor intensity of a worker.
Description
Technical field
The present invention relates to a kind of method that oligomeric isomaltose is produced, be specifically related to utilize ultra-filtration membrane recovery transglucosidase to realize the method for serialization production oligomeric isomaltose.
Background technology
Oligomeric isomaltose is functional oligose, is called as prebiotics, has the effect of breeding bifidus bacillus, improving intestinal microflora, because oligomeric isomaltose cost performance is high, is widely used in the industries such as food, healthcare products, milk-product, and market demand is huge.
At present the traditional production technique of oligomeric isomaltose intermittently saccharification turn glycosides reaction, after sugar component meets the requirements, adopt and heat up or adjust the material pH value enzyme that goes out, key enzyme preparation transglucosidase is wherein from Japanese import, expensive, traditional technology transglucosidase only utilizes once, and enzyme utilization ratio is low; Because adopting intermittently saccharification, traditional technology turns glycosides, saccharification still enormous amount, and area of mill site is large, and production line investment is high; Traditional technology concentration of substrate only 30%, the concentrated energy consumption of subsequent evaporation is high, and production cost is high; Between traditional technology saccharification still, influence factor is many, is unfavorable for controlling, and sugar component is unstable; Material impurity is many, need to carry out activated carbon decolorizing processing, and environmental pollution is larger; Production process operation is loaded down with trivial details, level of automation is low, and labor strength is large.
Summary of the invention
The present invention one of is intended to solve the problems of the technologies described above at least to a certain extent or at least provides a kind of useful business to select.
The present invention be intended to solve prior art problem one of at least.One aspect of the present invention provides a kind of method of producing oligomeric isomaltose, comprises the following steps:
A, take malt syrup as raw material, heat up, constant temperature sterilizing, cooling;
B, add transglucosidase, stir;
C, use ultrafiltration membrance filter, retain the oligomeric isomaltose solution filtering, and transglucosidase returns to saccharification still with concentrated solution, adds the malt syrup after processing of step A, adds transglucosidase simultaneously, repeating step B-C;
D, the oligomeric isomaltose solution filtering is adopted to the desalination of sun-male-female ion exchange process;
E, vacuum concentration obtain finished product.
According to concrete example of the present invention, in described steps A, malt syrup concentration is 40-45%, pH value 4-6.
According to concrete example of the present invention, in described steps A, be warming up to 85-95 ℃, keep 30 minutes, be then cooled to 58-60 ℃.
According to concrete example of the present invention, in described steps A, malt syrup is refining malt syrup, maltose content >=50%.
According to concrete example of the present invention, in described step B, adding after processing of step A is 10000: 6 with the ratio of transglucosidase.
According to concrete example of the present invention, after the sugar component in the medium question response container of described step C meets the requirements, carry out ultrafiltration membrance filter.The wherein general preferred saccharification still of reaction vessel.Meet the requirements and refer to detection sugar component, " trisaccharide " (isomaltose+panose+Isomaltotriose) >=35%.Ultrafiltration membrance filter can be selected the ultra-filtration membrane of molecular retention amount at 1-3 ten thousand.
According to concrete example of the present invention, in described step C, hyperfiltration process is cross flow filter mode.
Wherein cross flow filter (cross flow filtration) refers to that under the promotion of pump feed liquid is parallel to face and flows, the shearing force that feed liquid that different from dead-end filtration (dead-end flow filtration) is produces while flowing through face is taken away the particle being detained on face, thereby makes pollution layer remain on a thinner level.Cross flow filter operates compared with dead-end filtration complexity, and solid content is adopted to cross flow filter conventionally higher than 0.5% feed liquid.Along with the development of cross flow filter operative technique, there is in a lot of fields the trend that replaces dead-end filtration.
According to concrete example of the present invention, in described step C, add malt syrup after treatment in steps A, add 40% transglucosidase by enzyme rate of loss alive simultaneously.Wherein refer to according to malt syrup per ton and fill into 0.24KG transglucosidase by enzyme rate of loss alive.
According to concrete example of the present invention, in described step C, the component requirement of the oligomeric isomaltose after described filtration is: " trisaccharide " (isomaltose+panose+Isomaltotriose) >=35%, IMO content >=50%.
According to concrete example of the present invention, feeding temperature 35-55 ℃ in described step D, input concentration 40-45%, discharging pH value 4-6.
According to concrete example of the present invention, in described step D, zwitterion resin is respectively strong acidic ion resin and weak anion resin.Wherein strong acidic ion resin include, but are not limited to 723, D72, D301, D001 etc.Weak anion resin includes, but are not limited to D135, D900, D301,335 etc.
According to concrete example of the present invention, in described step e, adopt the board-like vacuum-evaporator of quadruple effect to concentrate filtrate after ion-exchange, product sugar is dense >=and 75%.
The method has realized saccharification and has turned glycosides reaction and carry out continuously, and production efficiency is high; Ultrafiltration membrance filter is cross flow filter technology, and transglucosidase can directly return to saccharification still, recycles and reuses, and unit enzyme productive rate is high, and production cost is low; Reaction substrate concentration is high, and rear road evaporization process energy consumption is low; Without traditional decoloration process, save gac, reduce the pollution to environment; Continuous production technology is stable, is easy to control, and can effectively guarantee quality product; Automation degree of equipment is high, and labor strength is little.
Additional aspect of the present invention and advantage in the following description part provide, and part will become obviously from the following description, or recognize by practice of the present invention.
Accompanying drawing explanation
Fig. 1 produces oligomeric isomaltose process flow sheet
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example is only for the present invention is described, and should not be considered as limiting scope of the present invention.
Embodiment mono-
A) raw material sterilizing: malt syrup is adjusted to sugar dense 43%, pH value 5.1, preparation 240L, is warming up to 90 ℃, maintenance 30min.
B) saccharification of saccharification still turns glycosides: the syrup after sterilizing is cooled to 60 ℃, adds 0.6kg transglucosidase, saccharification to turn glycosides 24 hours in malt syrup per ton.
C) ultra-filtration membrane reclaims transglucosidase continuously: detect sugar component, " trisaccharide " 35.89%, starting device for ultrafiltration membrane filters, the 10L syrup that leaches per hour, the 10L malt syrup that adds again per hour, add by malt syrup 0.24kg transglucosidase per ton simultaneously, continue to stir the transglucosidase rate of recovery >=60%.
D) ion-exchange: the syrup after filtration is cooled to 45 ℃, enters positive cation and anion exchange system and carries out desalination, and discharging electricity is led 20 μ S/cm, discharging pH value 5.2.
E) vacuum concentration: vacuum tightness is-0.08MPa to be concentrated into 75.6%, pH value 5.16.
Embodiment 2
A) raw material sterilizing: malt syrup is adjusted to sugar dense 45%, pH value 6.0, preparation 240L, is warming up to 85 ℃, maintenance 30min.
B) saccharification of saccharification still turns glycosides: the syrup after sterilizing is cooled to 59 ℃, and malt syrup per ton adds 0.6kg transglucosidase, and saccharification turns glycosides 24 hours.
C) ultra-filtration membrane reclaims transglucosidase continuously: detect sugar component, " trisaccharide " 35.77%, starting device for ultrafiltration membrane filters, the 10L syrup that leaches per hour, the 10L malt syrup that adds again per hour, add by malt syrup 0.24kg transglucosidase per ton simultaneously, continue to stir the transglucosidase rate of recovery >=60%.
D) ion-exchange: the syrup after filtration is cooled to 55 ℃, enters positive cation and anion exchange system and carries out desalination, and discharging electricity is led 18 μ S/cm, discharging pH value 5.9.
E) vacuum concentration: vacuum tightness is-0.078MPa to be concentrated into 75.4%, pH value 5.82.
Embodiment 3
A) raw material sterilizing: malt syrup is adjusted to sugar dense 40%, pH value 4.2, preparation 240L, is warming up to 90 ℃, maintenance 30min.
B) saccharification of saccharification still turns glycosides: the syrup after sterilizing is cooled to 58 ℃, and malt syrup per ton adds 0.6kg transglucosidase, and saccharification turns glycosides 24 hours.
C) ultra-filtration membrane reclaims transglucosidase continuously: detect sugar component, " trisaccharide " 35.56%, starting device for ultrafiltration membrane filters, the 10L syrup that leaches per hour, the 10L malt syrup that adds again per hour, add by malt syrup 0.24kg transglucosidase per ton simultaneously, continue to stir the transglucosidase rate of recovery >=60%.
D) ion-exchange: the syrup after filtration is cooled to 38 ℃, enters positive cation and anion exchange system and carries out desalination, and discharging electricity is led 15 μ S/cm, discharging pH value 4.5.
E) vacuum concentration: vacuum tightness is-0.082MPa to be concentrated into 75.3%, pH value 4.4.
Above about specific descriptions of the present invention, only for being described, the present invention is not limited to the technical scheme that embodiment of the present invention rope is described, those of ordinary skill in the art should be appreciated that still and can modify or be equal to replacement the present invention, reached constructed effect; Use needs as long as meet, all within protection scope of the present invention.
Claims (10)
1. a method of producing oligomeric isomaltose, is characterized in that, comprises the following steps:
A, take malt syrup as raw material, heat up, constant temperature sterilizing, cooling;
B, add transglucosidase, stir;
C, use ultrafiltration membrance filter, retain the oligomeric isomaltose solution filtering, and transglucosidase returns to reaction vessel with concentrated solution, adds the malt syrup after processing of step A, adds transglucosidase simultaneously, repeating step B-C;
D, the oligomeric isomaltose solution filtering is adopted to the desalination of sun-male-female ion exchange process;
E, vacuum concentration obtain finished product.
2. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, in steps A, malt syrup concentration is 40-45%, pH value 4-6.
3. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, is warming up to 85-95 ℃ in steps A, keeps 30 minutes, is then cooled to 58-60 ℃.
4. the method for production oligomeric isomaltose according to claim 1, is characterized in that: the malt syrup adding in described steps A is refining malt syrup, maltose content >=50%.
5. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, in step B, adding malt syrup after processing of step A and the ratio of transglucosidase is 10000: 6.
6. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, needs, after " trisaccharide " >=35% in waiting step B, to carry out ultrafiltration membrance filter in step C.
7. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, in step C, hyperfiltration process is cross flow filter mode.
8. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, adds malt syrup after treatment in steps A in step C, adds 40% transglucosidase by enzyme rate of loss alive simultaneously.
9. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, feeding temperature 35-55 ℃ in step D, input concentration 40-45%, discharging pH value 4-6.
10. the method for production oligomeric isomaltose as claimed in claim 1, is characterized in that, in step e, adopts the board-like vacuum-evaporator of quadruple effect to concentrate filtrate after ion-exchange, product sugar is dense >=and 75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210452471.7A CN103805654A (en) | 2012-11-13 | 2012-11-13 | Method for producing isomalto-oligosaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210452471.7A CN103805654A (en) | 2012-11-13 | 2012-11-13 | Method for producing isomalto-oligosaccharide |
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| CN103805654A true CN103805654A (en) | 2014-05-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210452471.7A Pending CN103805654A (en) | 2012-11-13 | 2012-11-13 | Method for producing isomalto-oligosaccharide |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102676616A (en) * | 2012-05-16 | 2012-09-19 | 成都连接流体分离科技有限公司 | Efficient and environment-friendly malt syrup producing process |
| CN102690851A (en) * | 2011-03-23 | 2012-09-26 | 山东百龙创园生物科技有限公司 | Method for producing oligoisomaltose by maltose |
-
2012
- 2012-11-13 CN CN201210452471.7A patent/CN103805654A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102690851A (en) * | 2011-03-23 | 2012-09-26 | 山东百龙创园生物科技有限公司 | Method for producing oligoisomaltose by maltose |
| CN102676616A (en) * | 2012-05-16 | 2012-09-19 | 成都连接流体分离科技有限公司 | Efficient and environment-friendly malt syrup producing process |
Non-Patent Citations (1)
| Title |
|---|
| 卢庭婷: "低聚异麦芽糖的生产和发展应用研究", 《轻工科技》 * |
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Address after: 251200 Shandong province Dezhou Dexin Street (Yucheng) National hi tech Industrial Development Zone Applicant after: Shandong Bailong Park biological Polytron Technologies Inc Address before: Dickson street 251200 Shandong city of Dezhou province Yucheng city high tech Development Zone Applicant before: Shandong Bailong Chuangyuan Biotechnology Co.,Ltd. |
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