CN103805509A - Simple and easy cell culture room - Google Patents
Simple and easy cell culture room Download PDFInfo
- Publication number
- CN103805509A CN103805509A CN201210519172.0A CN201210519172A CN103805509A CN 103805509 A CN103805509 A CN 103805509A CN 201210519172 A CN201210519172 A CN 201210519172A CN 103805509 A CN103805509 A CN 103805509A
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- China
- Prior art keywords
- top board
- cell culture
- round holes
- room
- board
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a simple and easy cell culture room. The simple and easy cell culture room comprises a bottom board, a top board, fixing bolts, an anti-release pin, a glass slide, a cell room, a pressurizing sleeve and a sealing cover, wherein the bottom board and the top board are of a groove-shaped structure; two round holes are formed in the bottom face of each groove-shaped structure; furthermore, a thread is arranged on the bottom face of each groove-shaped structure; two holes are respectively formed in each of the both ends of the two lateral faces of each groove-shaped structure; when the bottom board is buckled with an opening face of the groove-shaped structure, the round holes of the bottom faces of the bottom board and the top board are coaxial round holes, and the round holes on the lateral faces of the bottom board and the top board are coaxial round holes; the fixing bolts pass through the round holes so that the top board and the bottom board are combined; furthermore, the fixing bolts are prevented from releasing due to the anti-release pin; the pressurizing sleeve and the cell room are cylindrical; the pressurizing sleeve is sleeved outside the cell room; the thread matched with the round holes on the bottom face of the top board is arranged on the outer surface of the pressurizing sleeve; the outer diameter of the end, which is in contact with the glass slide, of the cell room, is large, so as to form a ring shape. Therefore, the cell room can be pushed to the glass slide, so that a cell culture fluid is prevented from being leaked; and the sealing cover covers the pressurizing sleeve. The simple and easy cell culture room provided by the invention has the advantages of simple structure and low manufacture cost, and can be repeatedly used.
Description
Technical field
The present invention relates to biological study instrument field, particularly Tissue Culture Dish.
Background technology
Cell culture chamber provides convenience for biological detection, but because of its disposable use and expensive its range of application that affected.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Simple cell culture compartment, detachable after using, cleaning, sterilization, Reusability.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme: comprise base plate, top board, gim peg, locking pin, slide glass, Cytology Lab, compressive sleeve, screw shell, nut, gasket, capping and slide glass.Described base plate and top board are bathtub construction, and base plate cross section is less than top board, and can by its contain into, the bottom surface of bathtub construction is provided with 2 circular holes, base plate and top board are that the two ends, two sides of bathtub construction are equipped with two circular holes, in the time of base plate and top board bathtub construction opening surface fastening, wherein base plate is coaxial circular hole with the circular hole of the bottom surface of top board, base plate is coaxial circular hole with the circular hole at the two ends of two sides of top board, after gim peg can pass, can be by top board and base slab stability combination, locking pin inserts gim peg and prevents that it from deviating from; Described screw shell, compressive sleeve and Cytology Lab are round shape, all have screw thread inside and outside screw shell, and its outside screw mates with nut, and its internal thread mates with compressive sleeve outer surface thread; Screw shell passes the bottom hole of top board and fixes by nut locking, compressive sleeve is inserted in screw shell, Cytology Lab is placed in screw shell, there is ring structure one end down, have gasket between between Cytology Lab and slide glass, compressive sleeve moves up and down in screw shell by helicitic texture, and compressive sleeve can be pushed Cytology Lab crush seal and be padded on slide glass, prevent that cell culture fluid from spilling, capping will be placed on compressive sleeve.The present invention is simple in structure, low cost of manufacture, can Reusability.
Preferably, the cross section of described bathtub construction is rectangle.
Accompanying drawing explanation
Fig. 1 is the assembly drawing of a kind of Simple cell culture compartment of the present invention.
Fig. 2 is the exploded view of a kind of Simple cell culture compartment of the present invention.
Embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described:
The easy cell culture chamber of one shown in Fig. 1 comprises base plate 1, top board 2, gim peg 3,4, locking pin 5,6, Cytology Lab 7,8, compressive sleeve 9,10, screw shell 13,14, nut 15,16, gasket 17,18, capping 11,12 and slide glass 13.The preferred structure of base plate and top board is: the cross section of its bathtub construction is rectangle, and the bottom surface of bathtub construction is provided with two isometrical symmetric offset spread circular holes.
The present invention also comprises a gim peg, and its preferred structure is cylindrical, and its diameter is consistent with base plate and top board bathtub construction side circular hole, but one end expands, and the other end is porose for inserting locking pin.
The present invention also comprises a screw shell 13,14, is round shape, inside and outside screw shell 13,14, all has screw thread, and its outside screw mates with nut 15,16, and its internal thread mates with compressive sleeve 9,10 outer surface threads.
The present invention also comprises a gasket 17,18, and its preferred structure is annular, between between Cytology Lab and slide glass.
Mounting means of the present invention is as follows: by two screw shells 13, 14 penetrate respectively in two circular holes of top board 2 bottom surfaces, and with nut 15, 16 are locked, compressive sleeve 9, 10 are screwed into screw shell 13, in 14, Cytology Lab 7, 8 are placed on compressive sleeve Nei Qinei, there is ring structure one end down, by gasket 17, 18 are pressed on contact slide glass 13, after top board 2 and base plate are fastened, by gim peg 3, 4 penetrate in base plate 1 and top board 2 two ends, two sides of bathtub construction circular holes the base plate of fastening 1 top board 2 are fixedly connected with, locking pin 5, 6 insert gim peg 3, slip-off preventing in 4, by compressive sleeve 9, 10 with screw shell 13, screwed between 14, further push Cytology Lab 7, 8 densification packings 17, 18 on slide glass 13, by capping 11, 12 are placed on compressive sleeve 9, on 10.
Claims (3)
1. an easy cell culture chamber, is characterized in that comprising base plate 1, top board 2, gim peg 3,4, locking pin 5,6, Cytology Lab 7,8, compressive sleeve 9,10, screw shell 13,14, nut 15,16, gasket 17,18, capping 11,12 and slide glass 13.Described base plate 1 and top board 2 are by gim peg 3,4 and the stable connection of locking pin 7,8; Screw shell 13,14 is placed in the hole of top board 2 bottom surfaces, and is locked with nut 15,16, and compressive sleeve 9,10 is pushed Cytology Lab 7,8 densification packings 17,18 on slide glass by being threaded into pressurization, prevents that cell culture fluid from spilling.
2. the easy cell culture chamber of one according to claim 1, is characterized in that: inside and outside described screw shell 13,14, all have screw thread, its outside screw mates with nut 15,16, and its internal thread mates with compressive sleeve 9,10 outer surface threads; Screw shell 13,14 is fixed on the bottom hole of top board 2 by nut 15,16 lockings, and compressive sleeve 9,10 moves up and down in screw shell 13,14 by helicitic texture.
3. the easy cell culture chamber of one according to claim 1, is characterized in that: described gasket 17,18 is between between Cytology Lab 7,8 and slide glass 13, and in the time that Cytology Lab 7,8 is pressurized, densification packing 17,18, on slide glass, plays sealing function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210519172.0A CN103805509A (en) | 2012-11-08 | 2012-11-08 | Simple and easy cell culture room |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210519172.0A CN103805509A (en) | 2012-11-08 | 2012-11-08 | Simple and easy cell culture room |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103805509A true CN103805509A (en) | 2014-05-21 |
Family
ID=50702900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210519172.0A Pending CN103805509A (en) | 2012-11-08 | 2012-11-08 | Simple and easy cell culture room |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103805509A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1923369A (en) * | 2006-09-02 | 2007-03-07 | 江西特康科技有限公司 | Test tube rack |
| CN2878406Y (en) * | 2006-03-28 | 2007-03-14 | 兰州民科生物技术中心 | Animal cell culture rotary bottle |
| CN201186220Y (en) * | 2008-03-21 | 2009-01-28 | 于水清 | Racks for test tubes |
| CN102443540A (en) * | 2010-10-11 | 2012-05-09 | 蒋秀英 | Detachable cell culture chamber |
| CN202214366U (en) * | 2011-08-30 | 2012-05-09 | 李郁松 | Simple cell culture compartment |
-
2012
- 2012-11-08 CN CN201210519172.0A patent/CN103805509A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2878406Y (en) * | 2006-03-28 | 2007-03-14 | 兰州民科生物技术中心 | Animal cell culture rotary bottle |
| CN1923369A (en) * | 2006-09-02 | 2007-03-07 | 江西特康科技有限公司 | Test tube rack |
| CN201186220Y (en) * | 2008-03-21 | 2009-01-28 | 于水清 | Racks for test tubes |
| CN102443540A (en) * | 2010-10-11 | 2012-05-09 | 蒋秀英 | Detachable cell culture chamber |
| CN202214366U (en) * | 2011-08-30 | 2012-05-09 | 李郁松 | Simple cell culture compartment |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858285A (en) * | 2017-12-13 | 2018-03-30 | 刘延群 | Split type bioreactor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140521 |