CN1038020A

CN1038020A - Experimental thrombus in vivo forms analyzer

Info

Publication number: CN1038020A
Application number: CN 88103071
Authority: CN
Inventors: 覃建民; 石山; 李增晞; 付洁民
Original assignee: Individual
Current assignee: Individual
Priority date: 1988-05-28
Filing date: 1988-05-28
Publication date: 1989-12-20
Anticipated expiration: 2003-05-28
Also published as: CN1041598C

Abstract

The present invention＜experimental thrombus in vivo forms analyzer〉be to measure thrombotic optical viewer in the animal body.When this instrument adopts unidirectional current to continue to stimulate the animal arteries to make it form the thrombosis plug flow, blood vessel far-end temperature bust, temperature sensor monitors blood vessel surface variations in temperature report to the police by this instrument and automatically record begin to the required correct time of temperature bust (being thrombosis) from stimulation, thereby inquire into medicine to the thrombotic effect and the mechanism of action of inquiring into its treatment cerebrovascular.Be applicable to each universities and colleges, hospital, scientific research institution.General instrument and meter factory and radio factory all can produce.

Description

Experimental thrombus in vivo forms analyzer

The present invention＜experimental thrombus in vivo forms analyzer〉belong to the device of physical therapy appliance moderate stimulation body internal reflection point.

The generation of artery thrombosis and cardiovascular and cerebrovascular disease is closely related with development, measures in the body that to form with external thrombus be a significant observation index in the experimentation.Because thrombosis is subjected to multiple factor affecting, and thrombus in vivo formation is difficult to direct observation again.In recent years, obtaining bigger progress aspect the antithrombotic research, form but observation index is limited to external thrombus, and the research that relevant thrombus in vivo forms report is being less more.1971 by J.Hladovec deliver＜continuous record rat experiment artery thrombosis (Thromb Diath Haemor-rh.26:407,1971) only provide following report: " be applicable to the method for a kind of rat experiment artery thrombosis of drug test; experimental result shows has antithrombotic to form effect; but form with electricity irritation carotid artery hyperamization bolt, but a thermometer continuous record thrombosis process connected simultaneously." but this type of equipment and instrument does not have manufacturer production.In February, 1987; 1(2) Chinese J Pharmacol Toxicol has been delivered the pharmacology group Zhang Shizhen of Shandong Medical University, Wei Xinbing, Wu Baojie, yellow Wen Xingwen chapter:＜alkali is put forward each component of heparinoid to the thrombotic influence of rat experiment〉in mention test and the time can test with stimulator, stimulating electrode, thermister, the result shows when intra-arterial is filled out blood flow because of thrombosis is stifled, blood vessel far-end temperature bust.Begin to be called duration of congestion (Occlusition time is called for short OT) from stimulation to temperature bust required time.With OT length as judging pharmacodynamics index.Do the morphological examination of this section tremulous pulse at last.

More than report ， And does not have special automatic assay instrument, and just test with various single-pieces various countries at present, and test complexity is loaded down with trivial details, and accuracy is relatively poor, operates very not aspect, and the application that especially can not automatization cooperatively interacts brings burden to experiment.

The present invention＜experimental thrombus in vivo forms analyzer〉and method can reach the automatization that this experiment is controlled.

This instrument causes the tunica intima damage with the lasting stimulation of unidirectional current blood vessel, activates platelet and blood coagulation system, and the PGI2 of while vascular endothelial cell injury synthesizes and release reduces, and causes and forms platelet thrombus and fibrinous thrombus in the arteries gradually.When in the arteries during because of the thrombosis plug flow, make blood vessel far-end temperature bust, temperature sensor can be monitored the blood vessel surface variations in temperature, begin to the required time of temperature bust from stimulation by instrument warning and automatic record, i.e. the thrombotic time (Occlusition time duration of congestion OT)

The present invention is made up of the circuit that clocks (Fig. 1), temperature control circuit (Fig. 2), stimulation delayed alarm circuit (Fig. 3), power circuit (Fig. 4), circuit structure diagram (Fig. 5), temperature sensor and stimulating electrode etc., it is characterized in that when in the arteries during because of the thrombosis plug flow, make blood vessel far-end temperature bust, the blood vessel surface variations in temperature is then monitored in temperature sensor, and reporting to the police and write down automatically by instrument begins to the required time of temperature bust from stimulation.The feature of circuit (Fig. 1) of clocking is formed relaxation oscillator at timing circuit by BT33E, produces the second signal of per second 1HZ, and input is by 5G657 after shaping, and four decade computers that 5G632 and YS-13-3 form clock.Temperature control circuit (Fig. 2) is characterised in that and adopts BTS100 series of temperatures pick off that after two F007 amplification filtering, trigger the controllable silicon guide alarm circuit, warning circuit adopts music integrated circuit.Stimulate delayed alarm circuit (Fig. 3) to be characterised in that by 3CG7D and form common-base circuit, high output impedance has guaranteed the requirement of constant current output, regulates the W(Current Regulation) the may command output current intensity.Delay circuit is made of BT33E and controllable silicon, relay, and output current regularly being exported be divided into is 7 grades, is respectively 2,3,4,5,6,7 and 8 fens.The feature of power circuit (Fig. 4) be by 78XX and 79XX three end integrated stable voltage circuits constitute+24V ± 12V and+the 3V power supply.It is that 1.5～2.0 millimeters stainless steel bar constitutes that stimulating electrode is characterised in that with 1～2 millimeter spacing of two diameters.Circuit structure diagram (Fig. 5) is characterized in that realizing this instrument master control and finishes automatic control.

Through enforcement, selected following best-of-breed technology index:

1. stimulation delay selection: be divided into 7 grades, be respectively 2,3,4,5,6,7 and 8 fens.

2. stimulating current: 0-10mA is adjustable continuously, and linearity error is ± 10%.

3. timing range 0-9999 second, error 2%.

4. temperature control sensitivity is 0.3 ℃.

5. running voltage 50HZ, 220V, ± 5%.

6. but the working time continuous operation is more than 10 hours.

7. the working condition ambient temperature is more than 20 ℃.

During use: 1. earlier power supply, clock and sting energizing switch and pull out position to the pass, plug temperature probe and stimulating electrode.

2. the regulated power supply of attaching plug and 220V is connected, turned on the power switch the charactron display digit.Press the zero clearing knob, the numeral that makes demonstration is zero.Regulating zeroing turn-knob (coarse adjustment or fine tuning) makes the pointer of temperature control current table be displaced to 50 places to the right.Regulate Current Regulation turn-knob and delay switch, make Current Regulation arrive desirable strength, time-delay is to the required time.

3. open time switch, begin to clock, as timing just often, close time switch and zero clearing.Then stimulating electrode and temperature probe hook on the arteries of separator well, open timing and thorn energizing switch simultaneously, promptly beginning stimulates and clocks.

4. after stimulation stops, closing the thorn energizing switch and take off stimulating electrode.Regulate the zeroing turn-knob and make the pointer of temp controlled meter slowly get back to zero-bit, with the formation of intra-arterial thrombosis, the pointer of temp controlled meter is by zero-bit deflection to the left gradually, and instrument is reported to the police when reaching critical localisation, closes the switch instrument that clocks and stops to report to the police and timing.The time that record shows, be thrombus formation time.

The present invention changes a little also can transform blood coagulation self-clocking instrument and two pass automatic timing unit as.

Significance of the present invention is to utilize the effect of various medicines to the rat experiment artery thrombosis of measuring accurately of the function of this instrument, to inquire into the mechanism of action of its treatment cerebrovascular, will and give anti-human body artery thrombosis to further treatment human body cerebrovascular and have important value.

The present invention＜experimental thrombus in vivo forms analyzer〉range of application: be applicable to that each medical colleges and schools of junior college, medical scientific mechanism, each big-and-middle-sized hospital and outlying district utilize the unit or the small hospital of local medical material research drug action mechanism.

The in addition accessory that the present invention is used, domestic equal can buying made also simplyr, and generally instrucment and meter plant or radio factory all can make.

Claims

1, experimental thrombus in vivo formation analyzer is characterized in that by the circuit that clocks (Fig. 1), temperature control circuit (Fig. 2), stimulates delayed alarm circuit (Fig. 3), power circuit (Fig. 4) circuit structure diagram (Fig. 5), temperature sensor and stimulating electrode etc. to form, its characteristics are when in the arteries during because of the thrombosis plug flow, blood vessel far-end temperature is descended, the blood vessel surface variations in temperature is then monitored in temperature sensor, effect by each circuit of this instrument and manufacture method reach report to the police and automatically record begin to the required time of temperature bust from stimulation.

2, the circuit that clocks according to claim 1 (Fig. 1), temperature control circuit (Fig. 2) stimulate delayed alarm circuit (Fig. 3), power circuit (Fig. 4), circuit structure diagram (Fig. 5) it is characterized in that by above line construction reach make clock, controls automatically such as temperature control, stimulation, warning.

3, according to claim 1, the 2 described circuit that clock (Fig. 1), it is characterized in that timing circuit forms relaxation oscillator by BT33E, produce the second signal of per second 1HZ, four decade computers that input is made up of 5G657,5G632 and YS-13-3 after shaping clock and this line construction.

4, according to claim 1,2 described temperature control circuits (Fig. 2), it is characterized in that temperature control circuit adopts BTS100 series of temperatures pick off, after two F007 amplification filtering, trigger the controllable silicon guide alarm circuit, warning circuit adopts music integrated circuit.

5, according to claim 1,2 described stimulation delayed alarm circuits (Fig. 3), it is characterized in that stimulation circuit forms common-base circuit by 3CG7D, high output impedance has guaranteed the requirement of constant current output, regulate the W(Current Regulation) the may command output current intensity, delay circuit is made of BT33E and controllable silicon, relay, output current is regularly exported, and being divided into is 7 grades, is respectively 2,3,4,5,6,7 and 8 fens.

6, according to claim 1,2 described power circuits (Fig. 4), it is characterized in that power circuit by 78XX and 79XX three end integrated stable voltage circuits constitute+24V, ± 12 and+the 3V power supply.

7, stimulating electrode according to claim 1 is characterized in that stimulating electrode with 1～2 millimeter of two diameter, and the stainless steel bar that spacing is 1.5～2.0 millimeters constitutes.

8, the described temperature sensor of  Huan ɡ  is characterized in that making its temperature control sensitivity in 0.3 ℃ by the circuit adjustment.

9, according to claim 1, the 2 described circuit that clock (Fig. 1), temperature control circuit (Fig. 2), the integrated circuit that stimulates delayed alarm circuit (Fig. 3), power circuit (Fig. 4) etc. all to be painted among the employing figure.