CN103787481A - Bioflocculation treatment method for tailing water - Google Patents
Bioflocculation treatment method for tailing water Download PDFInfo
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- CN103787481A CN103787481A CN201410019727.4A CN201410019727A CN103787481A CN 103787481 A CN103787481 A CN 103787481A CN 201410019727 A CN201410019727 A CN 201410019727A CN 103787481 A CN103787481 A CN 103787481A
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- paenibacillus polymyxa
- tail coal
- coal water
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Abstract
The invention discloses a bioflocculation treatment method for tailing water, and relates to the field of microbial technologies. The method comprises the following steps: 1, selecting paenibacillus polymyxa as a strain, inoculating the strain into a liquid medium, and culturing the strain in an oscillating incubator so as to obtain a formulated concentrate of a paenibacillus polymyxa culture solution; 2, centrifuging the formulated concentrate of the paenibacillus polymyxa culture solution on a centrifugal machine so as to obtain centrifugal liquid supernatant of the formulated concentrate of the paenibacillus polymyxa culture solution; adding 1-3 mL of the centrifugal liquid supernatant of the formulated concentrate of the paenibacillus polymyxa culture solution and 3-5 mL of a coagulant aid into tailing water, stirring the obtained product, and adjusting the pH value of the tailing water to 5-7. According to the invention, a coal bioflocculant prepared by using a paenibacillus polymyxa culture solution has a good treatment effect on tailing water of coal preparation plants, and has no side effect on people and animals; the flocculant can be self-degraded, so that no secondary pollution problem exists.
Description
Technical field
The invention belongs to microbial technology field, particularly a kind of biofloculation treatment process of tail coal water, especially suitable coal separating plant is used.
Background technology
Tail coal water claims again black water, belongs to the waste water after coal separation.Coal separating plant's tail coal water purification is a difficult problem for puzzlement coal separating plant always.Along with the raising of coal preparation machine degree, the shared proportion of Bed for Fine Coal is increasing.Tail coal water has been concentrated in raw coal the most carefully, the most unmanageable subparticle, because these grain graininess are thin, ash content is high, viscosity is large, be difficult to sedimentation, thereby conventional precipitation, recovery and dehydration equipment processing for extremely difficult.For this reason, flocculation agent is applied and has been produced certain effect in tail coal water treatment.
In tail coal water treatment, conventional flocculation agent has inorganic polymer flocculant, natural macromolecule flocculating agent, synthetic polymeric flocculant at present, but all there is certain deficiency in these flocculation agents: inorganic flocculating agent exist processing efficiency not good, expend large, have certain harm, and bring again the problems such as another kind of pollutent in eliminating a kind of pollutent; Polymeric flocculant, as polyacrylamide residual monomer in water is difficult for being degraded, has strong neurotoxicity, and also has very strong " three cause " effect.And coal biological flocculant is the combination of biotechnology and flocculation technique, it is to utilize biotechnology, extraction from microbe or its secretory product, purifying and the one safety that obtains, efficient, and New Type Water Treatment Chemicals that can natural degradation.Because microbial flocculant not only can overcome the inorganic and intrinsic deficiency of Syn-Organic flocculants itself, and have produce bacterium be easy to get, efficient, nontoxic, to environment non-secondary pollution, the advantages such as easy to use, safe and flocculation is in extensive range, are more and more subject to various countries efforts at environmental protection person's attention.
Summary of the invention
In order to overcome the existing inorganic and above-mentioned deficiency of Syn-Organic flocculants in tail coal water treatment, the invention provides a kind of biofloculation treatment process of tail coal water.Especially suitable coal separating plant is used.
For this reason, the technical scheme of the biofloculation treatment process of a kind of tail coal water of the present invention is as follows:
A biofloculation treatment process for tail coal water, comprising:
Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 29 ℃～31 ℃, cultivates 35h～37h, obtains the stoste of Paenibacillus polymyxa nutrient solution;
Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution;
Step 3, to the centrifuged supernatant and the 3mL～5mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 1mL～3mL in tail coal water, stirs, and to adjust the pH value of tail coal water be 5～7.
Technique scheme is further elaborated:
The formula of described liquid nutrient medium is: peptone 4g～6g, extractum carnis 2g～4g, distilled water 900mL～1100mL; And adjusting pH value is 7～9.
Paenibacillus polymyxa is inoculated in Erlenmeyer flask, and is placed in incubator, and this incubator is placed in the shaking table vibration that rotating speed is 150r/min～170r/min.
Described coagulant aids is 2% CaCl by mass
2solution.
Beneficial effect of the present invention is: the processing to coal preparation waste water (tail coal water) has high efficiency, and tail coal water absorbancy is 0.393～0.752; This coal biological flocculant composition is mainly polysaccharide, through test, nontoxic, without any side effects to humans and animals; This coal biological flocculant can be degraded voluntarily, non-secondary pollution problem.
Embodiment
Below, by an embodiment to introduce the specific embodiment of the present invention.
Embodiment
A biofloculation treatment process for tail coal water, comprising:
Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 30 ℃, cultivates 36h, obtains the stoste of Paenibacillus polymyxa nutrient solution.The formula of described liquid nutrient medium is: peptone 5g, extractum carnis 34g, distilled water 1000mL; And adjusting pH value is 8.As Paenibacillus polymyxa being inoculated in the Erlenmeyer flask of 250ml of 100ml liquid nutrient medium, and be placed in incubator, this incubator is placed in the shaking table vibration that rotating speed is 160r/min.
Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution.
Step 3, to the centrifuged supernatant and the 4mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 2mL in tail coal water, low rate mixing, and to adjust the pH value of tail coal water be 6.Described coagulant aids is 2% CaCl by mass
2solution.
Introduce the present invention and be applied to the comparative determination data of coal separating plant's tail coal purifying water process and natural subsidence below.
1, tail coal water is taken from Shanxi Sima coal separating plant of Lu An eco-friendly power source limited-liability company floating tail-coal water.Coal slime size composition and ash content thereof are as table 1.Get 100mL black water and in 100mL graduated cylinder, leave standstill 30min, utilize the absorbance that UV-5100 ultraviolet can spectrophotometric determination black water supernatant liquor.The results are shown in Table 2.
2, the mensuration of coal separating plant's tail coal aquatic organism flocculating settling effect.
Being inoculated in the 250ml Erlenmeyer flask that fills accordingly 100ml liquid nutrient medium with inoculating needle picking Paenibacillus polymyxa from slant medium, is 160rpm at rotating speed, in 30 ℃ of constant-temperature shaking incubators, cultivates 36h.
90mL black water is placed in to beaker, adds 4mL W (CaCl
2)=2% coagulant aids, low rate mixing 1min, then add the broken liquid of 2mL Paenibacillus polymyxa nutrient solution, add tail coal water to 100mL, regulating the pH value of tail coal water is 6 o'clock again, with after magnetic stirring apparatus low rate mixing 1min, tail coal water is poured in 100mL graduated cylinder, observe tail coal water flocculation sedimentation situation, after standing 30min, draw a certain amount of supernatant liquor, under 660nm, survey its absorbancy with spectrophotometer.Using absorbancy as tail coal water flocculation effect of settling deliberated index.Revision test the results are shown in Table 3, table 4.
The obvious clarification that adds the tail coal-water ratio after biological flocculant not add, measures through aforesaid method, and its absorbancy is 0.393 ~ 0.752.Synopsis 2 tail coal water natural subsidence test-results, biofloculation successful.This flucculation process can be used as a kind of effectively biofloculation treatment process of coal separating plant's tail coal water.
Embodiments of the invention just illustrate its embodiment, do not lie in and limit its technical scheme.The technician of the industry can make some and revise under the inspiration of the present embodiment, and the equivalence of doing according to the scope of the claims of the present invention therefore all changes or modifies, and all belongs within the scope of Patent right requirement of the present invention.
Claims (4)
1. a biofloculation treatment process for tail coal water, is characterized in that, comprising:
Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 29 ℃～31 ℃, cultivates 35h～37h, obtains the stoste of Paenibacillus polymyxa nutrient solution;
Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution;
Step 3, to the centrifuged supernatant and the 3mL～5mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 1mL～3mL in tail coal water, stirs, and to adjust the pH value of tail coal water be 5～7.
2. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, the formula of described liquid nutrient medium is: peptone 4g～6g, extractum carnis 2g～4g, distilled water 900mL～1100mL; And adjusting pH value is 7～9.
3. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, Paenibacillus polymyxa is inoculated in Erlenmeyer flask, and is placed in incubator, and this incubator is placed in the shaking table vibration that rotating speed is 150r/min～170r/min.
4. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, described coagulant aids is 2% CaCl by mass
2solution.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104326576A (en) * | 2014-11-25 | 2015-02-04 | 安徽理工大学 | Biological flocculation treatment method for coal preparation waste water |
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
| CN111979154A (en) * | 2020-08-31 | 2020-11-24 | 天津市晶品环保工程有限公司 | Microbial flocculant component and preparation method thereof |
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| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102628066A (en) * | 2012-04-17 | 2012-08-08 | 山西大学 | Preparation method and application of microbial flocculant |
| CN103031262A (en) * | 2012-12-14 | 2013-04-10 | 浙江大学 | Paenibacillus sp. strain and microbial exopolysaccharide flocculant generated by same as well as application of flocculant |
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| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN102628066A (en) * | 2012-04-17 | 2012-08-08 | 山西大学 | Preparation method and application of microbial flocculant |
| CN103031262A (en) * | 2012-12-14 | 2013-04-10 | 浙江大学 | Paenibacillus sp. strain and microbial exopolysaccharide flocculant generated by same as well as application of flocculant |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104326576A (en) * | 2014-11-25 | 2015-02-04 | 安徽理工大学 | Biological flocculation treatment method for coal preparation waste water |
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
| CN110643654B (en) * | 2019-10-17 | 2021-05-04 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
| CN111979154A (en) * | 2020-08-31 | 2020-11-24 | 天津市晶品环保工程有限公司 | Microbial flocculant component and preparation method thereof |
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Application publication date: 20140514 |