CN103787481A - Bioflocculation treatment method for tailing water - Google Patents

Bioflocculation treatment method for tailing water Download PDF

Info

Publication number
CN103787481A
CN103787481A CN201410019727.4A CN201410019727A CN103787481A CN 103787481 A CN103787481 A CN 103787481A CN 201410019727 A CN201410019727 A CN 201410019727A CN 103787481 A CN103787481 A CN 103787481A
Authority
CN
China
Prior art keywords
paenibacillus polymyxa
treatment
tail coal
water
coal water
Prior art date
Application number
CN201410019727.4A
Other languages
Chinese (zh)
Inventor
高雪明
程志宏
刘文革
田驰
Original Assignee
山西潞安环保能源开发股份有限公司
广东罗尔科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 山西潞安环保能源开发股份有限公司, 广东罗尔科技有限公司 filed Critical 山西潞安环保能源开发股份有限公司
Priority to CN201410019727.4A priority Critical patent/CN103787481A/en
Publication of CN103787481A publication Critical patent/CN103787481A/en

Links

Abstract

The invention discloses a bioflocculation treatment method for tailing water, and relates to the field of microbial technologies. The method comprises the following steps: 1, selecting paenibacillus polymyxa as a strain, inoculating the strain into a liquid medium, and culturing the strain in an oscillating incubator so as to obtain a formulated concentrate of a paenibacillus polymyxa culture solution; 2, centrifuging the formulated concentrate of the paenibacillus polymyxa culture solution on a centrifugal machine so as to obtain centrifugal liquid supernatant of the formulated concentrate of the paenibacillus polymyxa culture solution; adding 1-3 mL of the centrifugal liquid supernatant of the formulated concentrate of the paenibacillus polymyxa culture solution and 3-5 mL of a coagulant aid into tailing water, stirring the obtained product, and adjusting the pH value of the tailing water to 5-7. According to the invention, a coal bioflocculant prepared by using a paenibacillus polymyxa culture solution has a good treatment effect on tailing water of coal preparation plants, and has no side effect on people and animals; the flocculant can be self-degraded, so that no secondary pollution problem exists.

Description

A kind of biofloculation treatment process of tail coal water

Technical field

The invention belongs to microbial technology field, particularly a kind of biofloculation treatment process of tail coal water, especially suitable coal separating plant is used.

Background technology

Tail coal water claims again black water, belongs to the waste water after coal separation.Coal separating plant's tail coal water purification is a difficult problem for puzzlement coal separating plant always.Along with the raising of coal preparation machine degree, the shared proportion of Bed for Fine Coal is increasing.Tail coal water has been concentrated in raw coal the most carefully, the most unmanageable subparticle, because these grain graininess are thin, ash content is high, viscosity is large, be difficult to sedimentation, thereby conventional precipitation, recovery and dehydration equipment processing for extremely difficult.For this reason, flocculation agent is applied and has been produced certain effect in tail coal water treatment.

In tail coal water treatment, conventional flocculation agent has inorganic polymer flocculant, natural macromolecule flocculating agent, synthetic polymeric flocculant at present, but all there is certain deficiency in these flocculation agents: inorganic flocculating agent exist processing efficiency not good, expend large, have certain harm, and bring again the problems such as another kind of pollutent in eliminating a kind of pollutent; Polymeric flocculant, as polyacrylamide residual monomer in water is difficult for being degraded, has strong neurotoxicity, and also has very strong " three cause " effect.And coal biological flocculant is the combination of biotechnology and flocculation technique, it is to utilize biotechnology, extraction from microbe or its secretory product, purifying and the one safety that obtains, efficient, and New Type Water Treatment Chemicals that can natural degradation.Because microbial flocculant not only can overcome the inorganic and intrinsic deficiency of Syn-Organic flocculants itself, and have produce bacterium be easy to get, efficient, nontoxic, to environment non-secondary pollution, the advantages such as easy to use, safe and flocculation is in extensive range, are more and more subject to various countries efforts at environmental protection person's attention.

Summary of the invention

In order to overcome the existing inorganic and above-mentioned deficiency of Syn-Organic flocculants in tail coal water treatment, the invention provides a kind of biofloculation treatment process of tail coal water.Especially suitable coal separating plant is used.

For this reason, the technical scheme of the biofloculation treatment process of a kind of tail coal water of the present invention is as follows:

A biofloculation treatment process for tail coal water, comprising:

Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 29 ℃~31 ℃, cultivates 35h~37h, obtains the stoste of Paenibacillus polymyxa nutrient solution;

Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution;

Step 3, to the centrifuged supernatant and the 3mL~5mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 1mL~3mL in tail coal water, stirs, and to adjust the pH value of tail coal water be 5~7.

Technique scheme is further elaborated:

The formula of described liquid nutrient medium is: peptone 4g~6g, extractum carnis 2g~4g, distilled water 900mL~1100mL; And adjusting pH value is 7~9.

Paenibacillus polymyxa is inoculated in Erlenmeyer flask, and is placed in incubator, and this incubator is placed in the shaking table vibration that rotating speed is 150r/min~170r/min.

Described coagulant aids is 2% CaCl by mass 2solution.

Beneficial effect of the present invention is: the processing to coal preparation waste water (tail coal water) has high efficiency, and tail coal water absorbancy is 0.393~0.752; This coal biological flocculant composition is mainly polysaccharide, through test, nontoxic, without any side effects to humans and animals; This coal biological flocculant can be degraded voluntarily, non-secondary pollution problem.

Embodiment

Below, by an embodiment to introduce the specific embodiment of the present invention.

Embodiment

A biofloculation treatment process for tail coal water, comprising:

Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 30 ℃, cultivates 36h, obtains the stoste of Paenibacillus polymyxa nutrient solution.The formula of described liquid nutrient medium is: peptone 5g, extractum carnis 34g, distilled water 1000mL; And adjusting pH value is 8.As Paenibacillus polymyxa being inoculated in the Erlenmeyer flask of 250ml of 100ml liquid nutrient medium, and be placed in incubator, this incubator is placed in the shaking table vibration that rotating speed is 160r/min.

Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution.

Step 3, to the centrifuged supernatant and the 4mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 2mL in tail coal water, low rate mixing, and to adjust the pH value of tail coal water be 6.Described coagulant aids is 2% CaCl by mass 2solution.

Introduce the present invention and be applied to the comparative determination data of coal separating plant's tail coal purifying water process and natural subsidence below.

1, tail coal water is taken from Shanxi Sima coal separating plant of Lu An eco-friendly power source limited-liability company floating tail-coal water.Coal slime size composition and ash content thereof are as table 1.Get 100mL black water and in 100mL graduated cylinder, leave standstill 30min, utilize the absorbance that UV-5100 ultraviolet can spectrophotometric determination black water supernatant liquor.The results are shown in Table 2.

 

2, the mensuration of coal separating plant's tail coal aquatic organism flocculating settling effect.

Being inoculated in the 250ml Erlenmeyer flask that fills accordingly 100ml liquid nutrient medium with inoculating needle picking Paenibacillus polymyxa from slant medium, is 160rpm at rotating speed, in 30 ℃ of constant-temperature shaking incubators, cultivates 36h.

90mL black water is placed in to beaker, adds 4mL W (CaCl 2)=2% coagulant aids, low rate mixing 1min, then add the broken liquid of 2mL Paenibacillus polymyxa nutrient solution, add tail coal water to 100mL, regulating the pH value of tail coal water is 6 o'clock again, with after magnetic stirring apparatus low rate mixing 1min, tail coal water is poured in 100mL graduated cylinder, observe tail coal water flocculation sedimentation situation, after standing 30min, draw a certain amount of supernatant liquor, under 660nm, survey its absorbancy with spectrophotometer.Using absorbancy as tail coal water flocculation effect of settling deliberated index.Revision test the results are shown in Table 3, table 4.

The obvious clarification that adds the tail coal-water ratio after biological flocculant not add, measures through aforesaid method, and its absorbancy is 0.393 ~ 0.752.Synopsis 2 tail coal water natural subsidence test-results, biofloculation successful.This flucculation process can be used as a kind of effectively biofloculation treatment process of coal separating plant's tail coal water.

Embodiments of the invention just illustrate its embodiment, do not lie in and limit its technical scheme.The technician of the industry can make some and revise under the inspiration of the present embodiment, and the equivalence of doing according to the scope of the claims of the present invention therefore all changes or modifies, and all belongs within the scope of Patent right requirement of the present invention.

Claims (4)

1. a biofloculation treatment process for tail coal water, is characterized in that, comprising:
Step 1, selecting Paenibacillus polymyxa is bacterial classification, and this bacterium is inoculated in liquid nutrient medium, in the shaking culture case of 29 ℃~31 ℃, cultivates 35h~37h, obtains the stoste of Paenibacillus polymyxa nutrient solution;
Step 2, the stoste of Paenibacillus polymyxa nutrient solution is centrifugal on whizzer, thus make the centrifuged supernatant of the stoste of Paenibacillus polymyxa nutrient solution;
Step 3, to the centrifuged supernatant and the 3mL~5mL coagulant aids that add the stoste of the above-mentioned Paenibacillus polymyxa nutrient solution of 1mL~3mL in tail coal water, stirs, and to adjust the pH value of tail coal water be 5~7.
2. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, the formula of described liquid nutrient medium is: peptone 4g~6g, extractum carnis 2g~4g, distilled water 900mL~1100mL; And adjusting pH value is 7~9.
3. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, Paenibacillus polymyxa is inoculated in Erlenmeyer flask, and is placed in incubator, and this incubator is placed in the shaking table vibration that rotating speed is 150r/min~170r/min.
4. the biofloculation treatment process of a kind of tail coal water according to claim 1, is characterized in that, described coagulant aids is 2% CaCl by mass 2solution.
CN201410019727.4A 2014-01-17 2014-01-17 Bioflocculation treatment method for tailing water CN103787481A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410019727.4A CN103787481A (en) 2014-01-17 2014-01-17 Bioflocculation treatment method for tailing water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410019727.4A CN103787481A (en) 2014-01-17 2014-01-17 Bioflocculation treatment method for tailing water

Publications (1)

Publication Number Publication Date
CN103787481A true CN103787481A (en) 2014-05-14

Family

ID=50663606

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410019727.4A CN103787481A (en) 2014-01-17 2014-01-17 Bioflocculation treatment method for tailing water

Country Status (1)

Country Link
CN (1) CN103787481A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104326576A (en) * 2014-11-25 2015-02-04 安徽理工大学 Biological flocculation treatment method for coal preparation waste water
CN110643654A (en) * 2019-10-17 2020-01-03 浙江大学 Method for producing polysaccharide by fermenting starch wastewater

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870958A (en) * 2010-05-10 2010-10-27 新疆农业科学院微生物应用研究所 Paenibacillus and application thereof to environmental engineering
CN102628066A (en) * 2012-04-17 2012-08-08 山西大学 Preparation method and application of microbial flocculant
CN103031262A (en) * 2012-12-14 2013-04-10 浙江大学 Paenibacillus sp. strain and microbial exopolysaccharide flocculant generated by same as well as application of flocculant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870958A (en) * 2010-05-10 2010-10-27 新疆农业科学院微生物应用研究所 Paenibacillus and application thereof to environmental engineering
CN102628066A (en) * 2012-04-17 2012-08-08 山西大学 Preparation method and application of microbial flocculant
CN103031262A (en) * 2012-12-14 2013-04-10 浙江大学 Paenibacillus sp. strain and microbial exopolysaccharide flocculant generated by same as well as application of flocculant

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
侯志翔 等: ""煤泥水处理用微生物絮凝剂培养的优化试验研究"", 《煤炭加工与综合利用》, no. 6, 30 June 2012 (2012-06-30) *
杨朝晖 等: ""多粘类芽孢杆菌GA1产絮凝剂的培养基和分段培养工艺"", 《环境科学》, vol. 27, no. 7, 31 July 2006 (2006-07-31) *
王涛: ""微生物多菌种对煤泥水的絮凝试验及絮凝机理研究"", 《中国优秀硕士学位论文全文数据库》, 19 December 2011 (2011-12-19) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104326576A (en) * 2014-11-25 2015-02-04 安徽理工大学 Biological flocculation treatment method for coal preparation waste water
CN110643654A (en) * 2019-10-17 2020-01-03 浙江大学 Method for producing polysaccharide by fermenting starch wastewater

Similar Documents

Publication Publication Date Title
Acién et al. Wastewater treatment using microalgae: how realistic a contribution might it be to significant urban wastewater treatment?
Gutiérrez et al. Harvesting microalgae from wastewater treatment systems with natural flocculants: effect on biomass settling and biogas production
Jia et al. Removal of nitrogen from wastewater using microalgae and microalgae–bacteria consortia
Kong et al. Culture of microalgae Chlamydomonas reinhardtii in wastewater for biomass feedstock production
Bhatia et al. Coagulation–flocculation process for POME treatment using Moringa oleifera seeds extract: optimization studies
de la Noüe et al. Biological tertiary treatment of urban wastewaters with chitosan-immobilized Phormidium
Pu et al. Preparation and application of a novel bioflocculant by two strains of Rhizopus sp. using potato starch wastewater as nutrilite
Kurane et al. Screening for and characteristics of microbial flocculants
US8308944B2 (en) System and method for treating wastewater via phototactic heterotrophic microorganism growth
Şirin et al. Harvesting the microalgae Phaeodactylum tricornutum with polyaluminum chloride, aluminium sulphate, chitosan and alkalinity-induced flocculation
Su et al. Comparison of nutrient removal capacity and biomass settleability of four high-potential microalgal species
De la Noüe et al. Biotreatment of anaerobically digested swine manure with microalgae
Yang et al. A novel bioflocculant produced by K lebsiella sp. and its application to sludge dewatering
CN101503709B (en) Method for preparing bioflocculation by Bacillus licheniformis
Lee et al. Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris
Deng et al. Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment
Wang et al. Kinetics of nutrient removal and expression of extracellular polymeric substances of the microalgae, Chlorella sp. and Micractinium sp., in wastewater treatment
De Godos et al. Coagulation/flocculation-based removal of algal–bacterial biomass from piggery wastewater treatment
Garg et al. Flotation of marine microalgae: effect of algal hydrophobicity
CN104230004B (en) A kind of biotechnological formulation processing glutamic acid fermentation waste water
Sam et al. Flocculating performances of exopolysaccharides produced by a halophilic bacterial strain cultivated on agro-industrial waste
Escapa et al. Nutrients and pharmaceuticals removal from wastewater by culture and harvesting of Chlorella sorokiniana
Lananan et al. Optimization of biomass harvesting of microalgae, Chlorella sp. utilizing auto-flocculating microalgae, Ankistrodesmus sp. as bio-flocculant
Udom et al. Harvesting microalgae grown on wastewater
Riaño et al. Optimization of chitosan flocculation for microalgal-bacterial biomass harvesting via response surface methodology

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20140514