CN103785063B - Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof - Google Patents
Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof Download PDFInfo
- Publication number
- CN103785063B CN103785063B CN201210431868.8A CN201210431868A CN103785063B CN 103785063 B CN103785063 B CN 103785063B CN 201210431868 A CN201210431868 A CN 201210431868A CN 103785063 B CN103785063 B CN 103785063B
- Authority
- CN
- China
- Prior art keywords
- collagen
- hyaluronic acid
- type
- titanium coating
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
Titanium coating of a kind of type i collagen/hyaluronic acid modified and preparation method thereof, described type i collagen/hyaluronic acid modified titanium coating adopts biochemistry method of modifying, layer-layer technology and covalently immobolization method are combined, has type i collagen/hyaluronic acid composite bed on titanium coating surface with covalently immobolization.Compared with the collagen/hyaluronic acid composite bed of physical absorption, while the present invention adopts layer-layer technique construction type i collagen/hyaluronic acid composite bed, impart composite bed more stable structure by covalently immobolization method, make the Modified Titanium coating of preparation have better, more effective biology performance.Preparation method technique of the present invention is simple, efficiency is high, favorable repeatability, for medical bio coating technology provides new method.
Description
Technical field
The present invention relates to titanium coating of a kind of type i collagen/hyaluronic acid modified and preparation method thereof, be specifically related to a kind of employing biochemistry method of modifying, layer-layer technology and covalently immobolization method are combined, titanium coating surface with covalently immobolization Modified Titanium coating having type i collagen/hyaluronic acid composite bed and preparation method thereof, belong to medical bio coating technology field.
Background technology
Biochemistry modification is a kind of biomaterial surface method of modifying that development in recent years is got up, and its mode is that bioactive molecule (as extracellular matrix components, enzyme, polypeptide etc.) is fixed on biomaterial surface, gives the biology performance that material is good.Compared with traditional surface modifying method, through the bone implant material of biochemistry modification, by being fixed on bioactive molecule and the cell direct effect on its surface, better biological function can be played.Collagen, hyaluronic acid and other extracellular matrix components can be applicable to the biochemical surfaces modification of titanio bone alternate material.
Extracellular matrix components is more, is the synergistic result of various composition on the impact of cell behavior.At the bone implant material surface preparation two kinds even various kinds of cell epimatrix composite bed that becomes to be grouped into, better biology performance can be obtained.Report in document that maximum methods preparing composite bed is layer-layer technology.Chen etc. (J.L.Chen, et al.Applied SurfaceScience.2009 (255): 6894-6900.) utilize layer-layer technology to secure collagen/heparin composite bed on titanium surface, obtain good effect.But this composite bed is prepared by physical adsorption process, less stable.(the Ying Huang such as Huang, on this basis et al.Acta Biomateriatia.2012 (8): 866-877.), construct the collagen/hyaluronic acid/RGD composite bed with half interpenetrating network structure, improve the stability of composite bed to a certain extent, but composite bed is still and is fixed on substrate surface by physical absorption, within a short period of time can slowly dissolve.
Summary of the invention
The object of this invention is to provide titanium coating of a kind of type i collagen/hyaluronic acid modified and preparation method thereof.In view of utilize covalently immobolization method can titanium surface fixing-stable bio-molecule layer, the present invention is on the basis of layer-layer technology, introduce covalently immobolization method, at titanium coating surface construction type i collagen/hyalomitome acids extracellular matrix, to obtain the excellent Modified Titanium coating of Stability Analysis of Structures, biology performance.
At this, the invention provides the titanium coating of a kind of type i collagen/hyaluronic acid modified, namely biochemistry method of modifying is adopted, layer-layer technology and covalently immobolization method are combined, has the titanium coating of the type i collagen of type i collagen/hyaluronic acid composite bed/hyaluronic acid modified on titanium coating surface with covalently immobolization.
Compared with the collagen/hyaluronic acid composite bed of physical absorption, while the present invention adopts layer-layer technique construction type i collagen/hyaluronic acid composite bed, impart composite bed more stable structure by covalently immobolization method, make the Modified Titanium coating of preparation have better, more effective biology performance.
On the other hand, the present invention also provides the preparation method of described titanium coating, comprises alkali treatment, silanization treatment and covalently immobolization type i collagen and covalently immobolization hyaluronic acid.
Preferably, described alkali treatment comprises: titanium coating being immersed concentration is at 60 ~ 80 DEG C, be incubated 6 ~ 12h in the NaOH solution of 5 ~ 10M.
Preferably, described silanization treatment adopts the aminopropyl triethoxysilane of 5 ~ 10% to be silanizing agent.
Described silanization treatment can adopt toluene to be solvent, heating reflux reaction 6 ~ 12h.
Preferably, described covalently immobolization type i collagen comprises and to be immersed in by the titanium coating through silanization treatment in type i collagen/acetic acid solution and to adopt 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide to be carboxyl activator.
More preferably, described covalently immobolization type i collagen can be in 1mg/mL type i collagen/5mM acetic acid solution in concentration, drips 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide is respectively 2 ~ 3.5mg/mL and 0.25 ~ 0.875mg/mL with the concentration making it in reaction system.The response time of described covalently immobolization type i collagen is preferably 0.25 ~ 1h.
Again, preferably, described covalently immobolization hyaluronic acid comprises and to be immersed in by the titanium coating through silanization treatment in hyaluronic acid/acetic acid solution and to adopt 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide to be carboxyl activator.
More preferably, described covalently immobolization hyaluronic acid can be in 1mg/mL hyaluronic acid/5mM acetic acid solution in concentration, drips 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide is respectively 2 ~ 3.5mg/mL and 0.25 ~ 0.875mg/mL with the concentration making it in reaction system.The described covalently immobolization hyaluronic response time is preferably 0.25 ~ 1h.
Preferably, described covalently immobolization type i collagen and covalently immobolization hyaluronic acid alternate cycles are carried out.
It is 0.5 ~ 2 hour that described alternating cyclical process is preferably each circulation time, and cycle-index is 3 ~ 10 times.
Preferably, before described silanization treatment, deionized water ripening is also comprised.
Described deionized water ageing can be incubated 3 ~ 7 days by the titanium coating through alkali treatment in deionized water 40 ~ 80 DEG C.
Present invention process is simple, efficiency is high, favorable repeatability.The titanium coating of type i collagen provided by the present invention/hyaluronic acid modified, has good stability and biology performance, solves problem existing in prior art, can improve the clinical effectiveness of titanium coating, for medical bio coating technology provides new method.
Accompanying drawing explanation
Fig. 1 is the surface topography of Modified Titanium coating: the titanium coating (TC-A) of (a) alkali treatment; B () utilizes simple layer-layer technology at the titanium coating (TC-A (C/H) of its surface construction type i collagen/hyaluronic acid (C/H) layer
6); C () binder course-layer technology and covalently immobolization method are at the titanium coating (TC-AA (C/H) of its surface construction C/H layer
6); Amplify 1000 times;
Fig. 2 is the infrared spectrum of Modified Titanium coating;
Fig. 3 is the fixed effect analysis of type i collagen: (a) fixing glue commercial weight measures; The stability maintenance analysis of (b) collagen;
Fig. 4 is the biology performance experiment of covalently immobolization type i collagen/hyaluronic acid titanium coating: the cell adhesion experiments of (a) hMSCs; The cell proliferation experiment of (b) hMSCs; The alkaline phosphatase activities test experience of (c) hMSCs cell differentiation.
Detailed description of the invention
Further illustrate the present invention below in conjunction with accompanying drawing and following detailed description of the invention, should be understood that following embodiment and/or accompanying drawing are only for illustration of the present invention, and unrestricted the present invention.
The present invention adopts layer-layer technology and covalent bond grafting method, type i collagen and hyaluronic acid is fixed on plasma spray coating titanium coating surface, thus obtains the titanium coating with the type i collagen of good biological performance/hyaluronic acid modified.Exemplarily, its concrete technology is as follows.
(1) preparation of titanium coating: utilize plasma spray technology to prepare titanium coating.
(2) alkali treatment: described titanium coating being immersed concentration is be incubated 6 ~ 12h in the NaOH solution of 5 ~ 10M at 60 ~ 80 DEG C, and available deionized water ultrasonic cleaning after taking out, ultrasonic cleaning can continue about 5 minutes.Here adopt the alkali liquor of NaOH as alkali treatment of 5 ~ 10M, but should to understand other suitable alkali liquor be also applicable, such as potassium hydroxide.
(3) deionized water ageing: be incubated 3 ~ 7 days by described alkali treatment titanium coating in deionized water 40 ~ 80 DEG C.A water can be changed every day, dry at last 40 DEG C.
(4) silanization: dry titanium coating being immersed in concentration is in aminopropyl triethoxysilane (the APS)/toluene solution of 5 ~ 10%, solution is heated to boiling, condensing reflux, reaction 6 ~ 12h, after cooling, use ethanol, deionized water ultrasonic cleaning 1-2 time respectively, vacuum drying.Here the aminopropyl triethoxysilane (APS) adopting 5 ~ 10% is silanizing agent, and employing toluene as solvent, but should understand other suitable silanizing agents, solvent is also applicable.
(5) covalently immobolization type i collagen/hyaluronic acid: described silanization titanium coating is immersed in type i collagen/acetic acid solution (such as concentration is 1mg/mL type i collagen/5mM acetic acid solution), be added dropwise to 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) aqueous solution (such as 120mg/mL) and N-hydroxy-succinamide (NHS) aqueous solution (such as 30mg/mL), EDC ultimate density is made to be 2 ~ 3.5mg/mL, NHS ultimate density is 0.25 ~ 0.875mg/mL, take out after reaction 0.25 ~ 1h, deionized water rinsing; And then be immersed in hyaluronic acid/acetic acid solution (such as concentration is 1mg/mL hyaluronic acid/5mM acetic acid solution).Be added dropwise to 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) aqueous solution (such as 120mg/mL) and N-hydroxy-succinamide (NHS) aqueous solution (such as 30mg/mL), EDC ultimate density is made to be 2 ~ 3.5mg/mL, NHS ultimate density is 0.25 ~ 0.875mg/mL, take out after reaction 0.25 ~ 1h, deionized water rinsing.
(6) layer-layer is fixed: above-mentioned covalently immobolization type i collagen/hyaluronic acid process alternate cycles carried out repeatedly, such as 3 ~ 10 times, each response time is preferably 0.25 ~ 1 hour, and each circulation time is preferably 0.5 ~ 2 hour, last washed with de-ionized water vacuum drying.
Exemplify embodiment below further to describe the present invention in detail.Should understand equally; following examples are only used to further illustrate the present invention; can not be interpreted as limiting the scope of the invention, some nonessential improvement that those skilled in the art's foregoing according to the present invention is made and adjustment all belong to protection scope of the present invention.The amount of the reagent that following example is concrete and concentration, response time and temperature are also only examples in OK range, namely, those skilled in the art can be done in suitable scope by explanation herein and select, and do not really want the concrete numerical value being defined in Examples below.
Embodiment 1
[preparation based on the Modified Titanium coating of surface construction type i collagen/hyaluronic acid (C/H) class extracellular matrix]
Utilize plasma spray technology to prepare titanium coating (being labeled as TC), prepared titanium coating immerses in 5M NaOH solution, at 80 DEG C, be incubated 12h, after deionized water ultrasonic cleaning, ageing 7 days at 60 DEG C in deionized water, changes a water every day, dry at 40 DEG C.Alkali treatment modifying titanium coating is labeled as TC-A.The sample TC-A of drying being immersed in concentration is in the APS/ toluene solution of 10%, and solution is heated to boiling, condensing reflux, reaction 12h.With ethanol ultrasonic cleaning 1 time after cooling, deionized water ultrasonic cleaning 2 times, each 5 minutes, vacuum drying.The titanium coating of silanization is labeled as TC-AA.Sample TC-AA being immersed in concentration is in 1mg/mL type i collagen/5mM acetic acid solution.Instill 120mg/mL EDC aqueous solution and each 1mL of 30mg/mL NHS aqueous solution in every 38mL collagen solution, making the final solubility of EDC be the final solubility of 3mg/mL, NHS is 0.75mg/mL.Take out after reaction 0.5, deionized water rinsing.Then being immersed in concentration is in 1mg/mL hyaluronic acid/5mM acetic acid solution.Be added dropwise to 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide (EDC) aqueous solution (120mg/mL) and N-hydroxy-succinamide (NHS) aqueous solution (30mg/mL), making the final solubility of EDC be the final solubility of 3mg/mL, NHS is 0.75mg/mL.Take out after reaction 0.5h, deionized water rinsing.Be immersed in the type i collagen solution containing EDC/NHS again, so circulation 6 times.After washed with de-ionized water, vacuum drying.Identified as samples is designated as TC-AA (C/H)
6.
Comparative example 1
[adopting simple layer-layer technology to prepare type i collagen/hyaluronic acid composite bed]
Sample TC-A is immersed in 1mg/mL type i collagen/5mM acetic acid solution, take out after 0.5h, deionization rinses, then being immersed in concentration is in 1mg/mL hyaluronic acid/5mM acetic acid solution, take out after 0.5h, then be immersed in collagen solution, so circulation 6 times, vacuum drying, identified as samples is designated as TC-A (C/H)
6.
Fig. 1 is the surface topography of Modified Titanium coating.As can be seen from the figure, TC-A (C/H)
6and with TC-AA (C/H)
6surface all defines one deck biomacromolecule thin film.
Fig. 2 is the infrared spectrum of Modified Titanium coating.In figure, the change of Si-O-Si and amino characteristic peak further discloses titanium coating surface construction C/H composite bed.
[the collagen fixed effect experiment of type i collagen/hyaluronic acid modified titanium coating]
Utilize sirius red staining, investigate collagen fixed effect from quantitative and stability maintenance two aspect: by sample TC-A (C/H)
6with TC-AA (C/H)
6be immersed in sirius red-saturated picric acid solution that concentration is 1mg/mL, take out after 2 hours, deionized water rinsing, vacuum drying.By the NaOH solution of 0.1M, dyestuff is eluted, then measure the absorbance of dyestuff-NaOH solution under 540nm with ultraviolet spectrophotometer, with absorbance-collagen quantity standard curve control, draw the amount of fixing collagen.By sample TC-A (C/H)
6with TC-AA (C/H)
6each 4 are immersed in tris-HCl solution, hatch 1,3,5,7 day respectively under 37 degree.With sirius red staining measuring samples surface residual collagen quantity.
It is the fixed effect analysis of type i collagen shown in Fig. 3.As can be seen from Fig. 3 (a): the fixing type i collagen amount of two kinds of modes is all at 600 μ g/cm
2above, and both be more or less the same.In Fig. 3 (b), TC-A (C/H)
6collagen in 6 days with regard to desorption totally, and TC-AA (C/H)
6collagen still remain with more than 86% after 10 days, show TC-AA (C/H)
6fixing Stability of Collagen is better.
[the biology performance experiment of type i collagen/hyaluronic acid modified titanium coating]
The biology performance of covalently immobolization type i collagen titanium coating is investigated: investigate hMSCs in the adhesion of material surface and propagation behavior with mtt assay with human marrow mesenchymal stem cell (hMSCs); Quantitative assay is carried out by the activity of BCA method to the alkali phosphatase (ALP) of hMSCs.
Fig. 4 is the biology performance experiment of type i collagen/hyaluronic acid modified titanium coating.As can be seen from the figure: compared with TC, the surface construction Modified Titanium coating of C/H class extracellular matrix, the adhesion (Fig. 4 (a)) of hMSCs can be promoted, breed the activity expression (Fig. 4 (c)) of (Fig. 4 (b)) and alkali phosphatase, there is better biology performance.And with TC-A (C/H)
6compare, TC-AA (C/H)
6facilitation effect better.Result shows: utilize layer-layer technology and covalently immobolization method, at the constitutionally stable C/H composite bed of titanium coating surface construction, can improve the biology performance of titanium coating better.
Industrial applicability: the present invention is by layer-layer technology and covalent bond grafting mode, type i collagen and hyaluronic acid are fixed on titanium coating surface, obtain the titanium coating of a kind of type i collagen/hyaluronic acid modified, there is good stability and biology performance, have broad application prospects in medical biotechnology coating technology field.
Claims (10)
1. the titanium coating of a type i collagen/hyaluronic acid modified, it is characterized in that, adopt biochemistry method of modifying, layer-layer technology and covalently immobolization method are combined, type i collagen/hyaluronic acid composite bed is had with covalently immobolization on titanium coating surface, described biochemistry method of modifying comprises alkali treatment, silanization treatment, covalently immobolization type i collagen and covalently immobolization hyaluronic acid, wherein, described covalently immobolization type i collagen and covalently immobolization hyaluronic acid alternate cycles are carried out, the aminopropyl triethoxysilane of described silanization treatment employing 5 ~ 10% is silanizing agent, described silanization treatment adopts toluene to be solvent, heating reflux reaction 6 ~ 12h.
2. the preparation method of a titanium coating according to claim 1, it is characterized in that, comprise alkali treatment, silanization treatment, covalently immobolization type i collagen and covalently immobolization hyaluronic acid, wherein, described covalently immobolization type i collagen and covalently immobolization hyaluronic acid alternate cycles are carried out, the aminopropyl triethoxysilane of described silanization treatment employing 5 ~ 10% is silanizing agent, and described silanization treatment adopts toluene to be solvent, heating reflux reaction 6 ~ 12h.
3. preparation method according to claim 2, it is characterized in that, described covalently immobolization type i collagen comprises and to be immersed in by the titanium coating through silanization treatment in type i collagen/acetic acid solution and to adopt 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide to be carboxyl activator.
4. preparation method according to claim 3, it is characterized in that, drip 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide and be respectively 2 ~ 3.5mg/mL and 0.25 ~ 0.875mg/mL with the concentration making it in reaction system, the response time of described covalently immobolization type i collagen is 0.25 ~ 1h.
5. the preparation method according to any one of claim 2 ~ 4, it is characterized in that, described covalently immobolization hyaluronic acid comprises and to be immersed in by the titanium coating through silanization treatment in hyaluronic acid/acetic acid solution and to adopt 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide to be carboxyl activator.
6. preparation method according to claim 5, it is characterized in that, drip 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide and N-hydroxy-succinamide and be respectively 2 ~ 3.5mg/mL and 0.25 ~ 0.875mg/mL with the concentration making it in reaction system, the described covalently immobolization hyaluronic response time is 0.25 ~ 1h.
7. preparation method according to claim 2, is characterized in that, each circulation time is 0.5 ~ 2 hour, and cycle-index is 3 ~ 10 times.
8. the preparation method according to any one of claim 2 ~ 4, is characterized in that, described alkali treatment comprises: titanium coating being immersed concentration is at 60 ~ 80 DEG C, be incubated 6 ~ 12h in the NaOH solution of 5 ~ 10M.
9. the preparation method according to any one of claim 2 ~ 4, is characterized in that, before described silanization treatment, also comprise deionized water ripening.
10. preparation method according to claim 9, is characterized in that, described deionized water ageing comprises: be incubated 3 ~ 7 days by the titanium coating through alkali treatment in deionized water 40 ~ 80 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210431868.8A CN103785063B (en) | 2012-11-01 | 2012-11-01 | Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210431868.8A CN103785063B (en) | 2012-11-01 | 2012-11-01 | Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103785063A CN103785063A (en) | 2014-05-14 |
| CN103785063B true CN103785063B (en) | 2015-08-19 |
Family
ID=50661394
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210431868.8A Expired - Fee Related CN103785063B (en) | 2012-11-01 | 2012-11-01 | Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103785063B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7031980B2 (en) * | 2017-03-06 | 2022-03-08 | 多木化学株式会社 | Collagen-titanium complex |
| CN114796623B (en) * | 2022-03-08 | 2023-04-07 | 四川大学 | Fully-degradable cardiac occluder coating for promoting myocardial tissue repair and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5037677A (en) * | 1984-08-23 | 1991-08-06 | Gregory Halpern | Method of interlaminar grafting of coatings |
| CN1173824A (en) * | 1995-02-07 | 1998-02-18 | 菲迪亚高级生物多聚合物有限公司 | Process for the coating of objects with hyaluronic acid, derivatives thereof, and natural and semisynthetic polymers |
| CN101433736A (en) * | 2007-11-16 | 2009-05-20 | 英特尔公司 | Biocompatible coatings for medical devices |
| CN102008751A (en) * | 2010-11-24 | 2011-04-13 | 北京道淼浩博科技发展有限公司 | Biodegradable stent composite material and preparation method thereof |
-
2012
- 2012-11-01 CN CN201210431868.8A patent/CN103785063B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5037677A (en) * | 1984-08-23 | 1991-08-06 | Gregory Halpern | Method of interlaminar grafting of coatings |
| CN1173824A (en) * | 1995-02-07 | 1998-02-18 | 菲迪亚高级生物多聚合物有限公司 | Process for the coating of objects with hyaluronic acid, derivatives thereof, and natural and semisynthetic polymers |
| CN101433736A (en) * | 2007-11-16 | 2009-05-20 | 英特尔公司 | Biocompatible coatings for medical devices |
| CN102008751A (en) * | 2010-11-24 | 2011-04-13 | 北京道淼浩博科技发展有限公司 | Biodegradable stent composite material and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| Fabrication and in vitro evaluation of the collagen/hyaluronic acid PEM coating crosslinked with functionalized RGD peptide on titanium;Ying Huang 等;《Acta Biomaterialia》;20111020;第8卷;第866-877页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103785063A (en) | 2014-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kazimierczak et al. | Novel chitosan/agarose/hydroxyapatite nanocomposite scaffold for bone tissue engineering applications: Comprehensive evaluation of biocompatibility and osteoinductivity with the use of osteoblasts and mesenchymal stem cells | |
| CN109675104B (en) | Preparation method of mineralized hydrogel and biomimetic mineralized bone repair material | |
| Nagao et al. | Decellularized human kidney cortex hydrogels enhance kidney microvascular endothelial cell maturation and quiescence | |
| Choi et al. | Decellularized extracellular matrix derived from porcine adipose tissue as a xenogeneic biomaterial for tissue engineering | |
| Silva et al. | Compositional and structural analysis of glycosaminoglycans in cell-derived extracellular matrices | |
| Visser et al. | Crosslinkable hydrogels derived from cartilage, meniscus, and tendon tissue | |
| Bruyneel et al. | Ambiguity in the presentation of decellularized tissue composition: the need for standardized approaches | |
| Li et al. | Effects of flow shear stress and mass transport on the construction of a large-scale tissue-engineered bone in a perfusion bioreactor | |
| Kang et al. | In vitro 3D model for human vascularized adipose tissue | |
| Johns et al. | On the subtle tuneability of cellulose hydrogels: implications for binding of biomolecules demonstrated for CBM 1 | |
| CN104874015B (en) | A kind of bacteria cellulose dressing with antibacterial bacteriostatic function and preparation method and application | |
| Ao et al. | Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings | |
| CN102108130A (en) | Surface biological functionalization method for hydrophobic medical high polymer materials | |
| CN101531740A (en) | Method for forming simulated cell outer layer membrane structure on surface of cross-linked chitosan | |
| Merna et al. | Differential β3 integrin expression regulates the response of human lung and cardiac fibroblasts to extracellular matrix and its components | |
| EP3967750A1 (en) | Three-dimensional tissue construct and method for producing same, and method for producing cell-containing composition | |
| CN103785066B (en) | Type I collagen modified porous titanium coat and making method thereof | |
| CN103785063B (en) | Titanium coating of type i collagen/hyaluronic acid modified and preparation method thereof | |
| CN104028434B (en) | A kind of method at the laminin/heparin/SDF-1 α anti-freezing of titanium surface construction and inducing endothelial Multifunctional layered | |
| Bosetti et al. | Poly‐L‐lactide acid‐modified scaffolds for osteoinduction and osteoconduction | |
| CN101905034A (en) | Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material | |
| CN102068965A (en) | Method for preparing chitosan separation medium suitable for protein purification | |
| Li et al. | Apatite formation induced by chitosan/gelatin hydrogel coating anchored on poly (aryl ether nitrile ketone) substrates to promote osteoblastic differentiation | |
| CN108359407A (en) | A kind of modified vegetable protein adhesive and preparation method thereof | |
| Yuan et al. | Self‐Healing, High Adherent, and Antioxidative LbL Multilayered Film for Enhanced Cell Adhesion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150819 Termination date: 20201101 |