CN103784738A - Extraction method and application of plant composition containing Maca - Google Patents
Extraction method and application of plant composition containing Maca Download PDFInfo
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- CN103784738A CN103784738A CN201410084330.3A CN201410084330A CN103784738A CN 103784738 A CN103784738 A CN 103784738A CN 201410084330 A CN201410084330 A CN 201410084330A CN 103784738 A CN103784738 A CN 103784738A
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- meyenii walp
- lepidinm meyenii
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- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 238000000605 extraction Methods 0.000 title claims abstract description 16
- 240000000759 Lepidium meyenii Species 0.000 title abstract description 6
- 235000000421 Lepidium meyenii Nutrition 0.000 title abstract description 6
- 235000012902 lepidium meyenii Nutrition 0.000 title abstract description 6
- 241000196324 Embryophyta Species 0.000 title abstract 4
- 239000003814 drug Substances 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 13
- 238000000874 microwave-assisted extraction Methods 0.000 claims abstract description 8
- 229940079593 drug Drugs 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 45
- 235000013311 vegetables Nutrition 0.000 claims description 26
- 208000032612 Glial tumor Diseases 0.000 claims description 12
- 206010018338 Glioma Diseases 0.000 claims description 12
- 101100496572 Rattus norvegicus C6 gene Proteins 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 10
- 239000002671 adjuvant Substances 0.000 claims description 7
- 230000000274 adsorptive effect Effects 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 230000006837 decompression Effects 0.000 claims description 7
- 239000003480 eluent Substances 0.000 claims description 7
- 238000010828 elution Methods 0.000 claims description 7
- 238000004064 recycling Methods 0.000 claims description 7
- 229920005989 resin Polymers 0.000 claims description 7
- 239000011347 resin Substances 0.000 claims description 7
- 239000008187 granular material Substances 0.000 claims description 6
- 239000002775 capsule Substances 0.000 claims description 5
- 239000003826 tablet Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 4
- 239000012567 medical material Substances 0.000 abstract description 2
- 241000213006 Angelica dahurica Species 0.000 abstract 1
- 235000017276 Salvia Nutrition 0.000 abstract 1
- 240000007164 Salvia officinalis Species 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 230000000452 restraining effect Effects 0.000 abstract 1
- 229960004756 ethanol Drugs 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 229920002334 Spandex Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000010165 autogamy Effects 0.000 description 1
- FCPVYOBCFFNJFS-LQDWTQKMSA-M benzylpenicillin sodium Chemical compound [Na+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)CC1=CC=CC=C1 FCPVYOBCFFNJFS-LQDWTQKMSA-M 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012738 dissolution medium Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004900 laundering Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 239000004759 spandex Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 238000009495 sugar coating Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an extraction method of a plant composition containing Maca. The plant composition is prepared from the raw medical materials of 100g of Maca, 80g of sealwort, 60g of Chinese angelica and 50g of red-rooted salvia roots through a microwave extraction method, so that the content of the Maca is greatly improved, and the use amount is reduced. The invention further provides an application of the plant composition containing the Maca in preparation of rat neurospongioma C6 cell proliferation restraining drugs.
Description
Technical field
The present invention relates to Chinese medicine preparation technical field, be specifically related to a kind of vegetable composition extracting method and application containing Lepidinm meyenii Walp.
Background technology
Contain the vegetable composition of Lepidinm meyenii Walp by Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g makes as crude drug, and current extracting method is to decoct with water secondary, each 2-3 hour, collecting decoction, filters, and is condensed into thick paste, granulates, dry, be pressed into 1000, sugar coating, to obtain final product, can resisting fatigue.
In prior art, not yet have containing the vegetable composition of Lepidinm meyenii Walp and extracting the report that adopts microwave technology aspect preparation, and the method that adopts decocting to boil, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide a kind of vegetable composition extracting method containing Lepidinm meyenii Walp.
Another object of the present invention is to provide a kind of vegetable composition containing Lepidinm meyenii Walp to suppress the application in rat C6 glioma cells propagation medicine in preparation.
Technical scheme: the object of the invention is to realize by following scheme:
A kind of vegetable composition extracting method containing Lepidinm meyenii Walp, vegetable composition is by Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into tablet, capsule or granule.
Above-mentioned a kind of vegetable composition extracting method containing Lepidinm meyenii Walp, in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
A kind of vegetable composition containing Lepidinm meyenii Walp suppresses the application in rat C6 glioma cells propagation medicine in preparation, contain the vegetable composition of Lepidinm meyenii Walp by Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into tablet, capsule or granule.
Above-mentioned a kind of vegetable composition containing Lepidinm meyenii Walp suppresses the application in rat C6 glioma cells propagation medicine in preparation, and the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
In prior art, containing every 0.25g of vegetable composition of Lepidinm meyenii Walp, each 4,3 times on the one, the every 0.25g of vegetable composition containing Lepidinm meyenii Walp that adopts the present invention to be prepared into, but the medical material amount containing is original 2 times, therefore only needs 2 at every turn, within 1st, take 3 times, under the condition with more active component, greatly reduced dose.
The specific embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract each 4-8 minute, combining extraction liquid 2 times, concentrated, be added on D101 macroporous adsorptive resins 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrates and is dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into tablet, every 0.25g.
Embodiment 2
Get Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into capsule.
Embodiment 3
Get Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extracts 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into granule.
In the present invention, Lepidinm meyenii Walp is the underground part root division of crucifer Lepidium meyenii, and all the other are pharmacopeia kind.
Embodiment 4: experimentation data 1 experiment material that suppresses rat C6 glioma cells propagation containing the vegetable composition of Lepidinm meyenii Walp
1.1 experiment cell strains
Rat C6 glioma cells, Nanjing Medical University's Experimental Animal Center laboratory cell bank, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: the present invention contains the vegetable composition of Lepidinm meyenii Walp: press embodiment 1 method preparation.
Medicinal liquid liquid storage: take the vegetable composition of 100mg containing Lepidinm meyenii Walp, be dissolved in 5ml dehydrated alcohol, 0.2 μ m filter filters, 500 μ l doff pipe subpackages ,-20 ℃ of storages, 0.2 μ m filter filters the use of dehydrated alcohol in order to matched group simultaneously.1.3 experiment reagent
The Cat.No.12100-061Lot.No.758137 of DMEM(GIBCO company); Hyclone (Lot.No.100419 of Tian Hang bio tech ltd, Zhejiang); NaHCO3(Shanghai hundred million Cat.No.11810-033Lot.No.1088387 of chemical reagent company limited of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); Penicillin G Sodium Salt(AMRESCO company lot number: 2010242); Streptomycin Sulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS(laboratory autogamy); 1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-ultraviolet light microwell plate detector (MD company of U.S. model: SPECTRA MAX190); CO2 incubator (FORMA model: 3111); Super-clean bench (safe and sound company of Su Jing group manufactures model: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (Shanghai accurate experimental facilities company model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μ m filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, pipet, Tips are some.
2 experimental techniques
1) rat C6 glioma cells carries out cellar culture (10cm culture dish) with DMEM+10%FBS in 37 ℃, 5%CO2, when Growth of Cells is during to logarithmic (log) phase, collecting cell, discards culture fluid, PBS fine laundering 3 times, add 3ml0.25% trypsin-0.04%EDTA, after 37 ℃ of digestion 2min, add wherein 5ml complete medium neutralization reaction, after piping and druming cell, proceeded in centrifuge tube, the centrifugal 5min of 1000rpm, adjusts 3 × 104/ml of concentration of cell suspension.2) cell kind is entered in 96 well culture plates, every hole adds cell suspension 180 μ l, and culture plate is put into cell culture incubator (37 ℃, 5%CO2) cellar culture.
3) according to Growth of Cells situation, generally grow to 50%-70%, add the vegetable composition solution containing Lepidinm meyenii Walp, continue to cultivate 24h.
4) after 24h, add 20 μ l MTT solution (5mg/ml, i.e. 0.5%MTT), continue to cultivate 4h.
5) after 4h, buckle method is removed supernatant, pats dry gently with absorbent paper, and every hole adds 200 μ l dimethyl sulfoxide, puts low-speed oscillation 10min on shaking table, and crystal is fully dissolved.Measure the light absorption value in each hole at enzyme-linked immunosorbent assay instrument 490nm place.6) background (do not add cell, only add culture fluid) is set simultaneously, control wells (the medicine dissolution medium of cell, same concentrations, culture fluid, MTT, dimethyl sulfoxide), sets 6 multiple holes for every group.
7) result represents the suppression ratio of cell with medicine:
Cell increment suppression ratio (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repeats 3 times.
3 statistical dispositions
Adopt correlation analysis and Student t check in Microsoft Excel2003 software, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, with matched group comparison, in the time that dosage reaches 5mg/ml, rat C6 glioma cells propagation is suppressed to variant (P<0.05), dosage this difference in the time of 10mg/ml has significance (P<0.01), has utmost point significant difference (P<0.001) in the time that dosage reaches 15-20mg/ml.
Table 1 is studied rat C6 glioma cells propagation inhibitory effect containing the vegetable composition of Lepidinm meyenii Walp
Note: with matched group comparison, * P<0.01; * P<0.001
5 experiment conclusion
Can suppress rat C6 glioma cells propagation containing the vegetable composition of Lepidinm meyenii Walp, reduce the Growth of Cells number of rat C6 glioma cells, this effect is dose dependent.
Claims (4)
1. the vegetable composition extracting method containing Lepidinm meyenii Walp, it is characterized in that vegetable composition is by Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into tablet, capsule or granule.
2. a kind of vegetable composition extracting method containing Lepidinm meyenii Walp according to claim 1, is characterized in that in preparation method, microwave extracting power is 500W, extracts 6 minutes at every turn.
3. the vegetable composition containing Lepidinm meyenii Walp suppresses the application in rat C6 glioma cells propagation medicine in preparation, it is characterized in that the vegetable composition that contains Lepidinm meyenii Walp is by Lepidinm meyenii Walp 100g, Rhizoma Polygonati 80g, Radix Angelicae Sinensis 60g, Radix Salviae Miltiorrhizae 50g makes as crude drug, preparation method is made up of the following step: get above-mentioned raw materials medicine, pulverize, add 70% ethanol of 2L, drop in microwave extracting apparatus and carry out microwave extracting, extraction power is 400-600W, extract 2 times, each 4-8 minute, combining extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collect 5 times of amount column volume eluents, decompression recycling ethanol, concentrate and be dried, obtain microwave extraction thing, add conventional adjuvant and be prepared into tablet, capsule or granule.
4. a kind of vegetable composition containing Lepidinm meyenii Walp suppresses the application in rat C6 glioma cells propagation medicine in preparation according to claim 3, it is characterized in that the power of microwave extracting described in preparation method is 500W, extracts 6 minutes at every turn.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104920762A (en) * | 2015-06-30 | 2015-09-23 | 福建德广中草药有限公司 | Huangjing Maka tablet sugar and production method thereof |
| CN105106264A (en) * | 2015-10-03 | 2015-12-02 | 陈爱华 | Extraction method and application of plant composition containing shiitake |
| CN105106331A (en) * | 2015-10-02 | 2015-12-02 | 陈爱华 | Extraction method and application of plant composition containing oenothera biennis |
| CN105125943A (en) * | 2015-10-03 | 2015-12-09 | 陈爱华 | Extracting method for plant composition containing American ginseng and application |
| CN105125989A (en) * | 2015-10-02 | 2015-12-09 | 陈爱华 | Extracting method and application of plant composite containing hawthorns |
| CN105147842A (en) * | 2015-10-02 | 2015-12-16 | 南京多宝生物科技有限公司 | Extracting method and application of plant composition containing radix pseudostellariae |
| CN105147869A (en) * | 2015-10-03 | 2015-12-16 | 陈爱华 | Extracting method and application of plant composition containing green tea |
| CN105193967A (en) * | 2015-09-12 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing cimicifugae foetidae and application |
| CN105194002A (en) * | 2015-09-16 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing echinacea and application of plant composition |
| CN105582132A (en) * | 2016-03-07 | 2016-05-18 | 南京多宝生物科技有限公司 | Preparation method and application of liquorice root formulation granules |
| CN105581333A (en) * | 2015-12-30 | 2016-05-18 | 重庆骄王天然产物股份有限公司 | Fully-nutritional Maca powder and preparation method thereof |
| CN106108008A (en) * | 2016-06-24 | 2016-11-16 | 丽江师范高等专科学校 | A kind of extracting method of effective component in Maka |
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| CN104920762A (en) * | 2015-06-30 | 2015-09-23 | 福建德广中草药有限公司 | Huangjing Maka tablet sugar and production method thereof |
| CN105193967A (en) * | 2015-09-12 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing cimicifugae foetidae and application |
| CN105194002A (en) * | 2015-09-16 | 2015-12-30 | 南京正亮医药科技有限公司 | Method for extracting plant composition containing echinacea and application of plant composition |
| CN105106331A (en) * | 2015-10-02 | 2015-12-02 | 陈爱华 | Extraction method and application of plant composition containing oenothera biennis |
| CN105125989A (en) * | 2015-10-02 | 2015-12-09 | 陈爱华 | Extracting method and application of plant composite containing hawthorns |
| CN105147842A (en) * | 2015-10-02 | 2015-12-16 | 南京多宝生物科技有限公司 | Extracting method and application of plant composition containing radix pseudostellariae |
| CN105106264A (en) * | 2015-10-03 | 2015-12-02 | 陈爱华 | Extraction method and application of plant composition containing shiitake |
| CN105125943A (en) * | 2015-10-03 | 2015-12-09 | 陈爱华 | Extracting method for plant composition containing American ginseng and application |
| CN105147869A (en) * | 2015-10-03 | 2015-12-16 | 陈爱华 | Extracting method and application of plant composition containing green tea |
| CN105581333A (en) * | 2015-12-30 | 2016-05-18 | 重庆骄王天然产物股份有限公司 | Fully-nutritional Maca powder and preparation method thereof |
| CN105582132A (en) * | 2016-03-07 | 2016-05-18 | 南京多宝生物科技有限公司 | Preparation method and application of liquorice root formulation granules |
| CN106108008A (en) * | 2016-06-24 | 2016-11-16 | 丽江师范高等专科学校 | A kind of extracting method of effective component in Maka |
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| CN103784738B (en) | 2016-04-13 |
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