CN103768700B - Antibacterial catheter of albumen and preparation method thereof - Google Patents
Antibacterial catheter of albumen and preparation method thereof Download PDFInfo
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Abstract
Present invention is disclosed the antibacterial catheter of a kind of albumen, it has one deck non-dispersive nanoscale on the surface of catheter bulk material lubricious, the bulk composition of this coating is harmless native protein, the thickness of coating is 10nm ~ 500nm, and the r.m.s. roughness of coating is not more than 100nm.Present invention further teaches the preparation method of this catheter, key step is: carry out pretreatment to catheter bulk material, preparation protein glue, with it, single or multiple coating is carried out to the surface of catheter bulk material, finally adopt the methods such as spontaneous curing, microwave curing, heat cure, ultraviolet light polymerization or crosslinking curing, the protein glue being attached to catheter bulk material surface is cured.By the catheter that technical solution of the present invention is obtained, it is antibacterial, antibacterial effect is excellent, can alleviate financial burden and physical pain that patient frequently changes catheter, and good stability in vivo, toxicity is low, can not bring some malaise symptoms, Clinical practice safety to patient.
Description
Technical field
The present invention relates to the preparation method of a kind of pharmaceutical antibacterial catheter and this catheter, specifically refer to use Antagonistic protein matter glue, make it form protein nano coating and be attached to catheter surface, and then obtained antibacterial catheter, thus use in vivo in this catheter process and effectively suppress the adhesion of various bacteria and mycete and grow.
Background technology
Medical urethral catheter is one of clinical the most frequently used medical device, though have a wide range of applications, but the infections relating that indwelling catheter causes is general stubborn problem again in clinical treatment, more easily there are the problems referred to above in the Preserving time type catheter especially accounting for total amount 10%, further, 75% ~ 80% is had to be caused by catheter in urinary tract infection.Tradition reply catheter cause infect method as: shorten the catheter intracorporeal indwelling time, periodic replacement; Strengthen hospital to Related Nursings such as catheters; Coordinate antibiotic etc take prevent or treat urinary tract infection etc.Said method not only makes patient economy burden and physical pain increase the weight of, and also increases treatment time and takies hospital's ample resources.
Existing antibacterial or antibacterial catheter, the coating mostly had by its surface and realize antibacterial or bacteria resistance function, mainly comprises metal ion/alloy coat or antibiotic medicine coating.Wherein, the catheter of surface attachment metal ion/alloy coat is mainly used for reaching antibacterial by the distinctive physical sterilization of metal, antibacterial object, when above-mentioned metal/alloy exists with nano-particle state, its germicidal efficiency is especially outstanding, as relevant report document " Self-sterilizingcatheterswithtitaniumdioxidephotocatalys tthinfilmsforcleanintermittentcatheterization:Basisandst udyofclinicaluse.InternationalJournalofUrology, 5 (14): 426-430 ", " Self-sterilizingandself-cleaningofsiliconecatheterscoate dwithTiO
2photocatalystthinfilms:apreclinicalwork.JournalofBiomedi calMaterialsResearch, 1 (58): 97-101 " and " Therelationshipbetweentheantimicrobialeffectofcatheterco atingscontainingsilvernanoparticlesandthecoagulationofco ntactingblood.Biomaterials, 22 (30): 3682-3690 ", the nanometer silver or disclosed in Chinese patent CN100518853C and CN1736504A or nano-titanium coating catheter.But the catheter manufacturing cost of such metal ion/alloy coat is high, expensive, easily causes urine mineralization on catheter, form hackly surface, bring misery to patient; And the medical safety of nano metal/alloy particle, especially nano silver particles exists dispute always, if it remains accumulation in vivo may cause heavy metal poisoning.
In addition, another kind of antibacterial, antibacterial antibiotic medicine coating catheter is that antibiotic medicine is direct or is indirectly coated in catheter surface by carrier, relies on antibiotic medicine to be released in body and produces antibacterial, fungistatic effect.Disclosed in Chinese patent CN101628133A, the antibiotic such as penicillin, head roe deer rhzomorph, macrolide are made antibacterial, antibiotic catheters as slow releasing pharmaceutical in conjunction with polyglycolic-polylactic acid carrier.Although this kind of catheter price is lower than metal ion/alloy coat catheter, but still there is following shortcoming: the antibiolics power persistence of slow release is more weak, life-time service may cause antibiotic resistant bacteria produce and affect its antibacterial, antibacterial efficacy.
Except above-mentioned two kinds antibacterial, antimicrobial coating catheter, also just like Chinese patent CN201375746, US Patent No. 7597903 and document " Chitosan/poly (vinylalcohol) blendinghydrogelcoatingimprovesthesurfaceCharacteristics ofsegmentedpolyurethaneurethralcatheters.JournalofBiomed icalMaterialsResearchPartB-AppliedBiomaterials, 2 (83B): 304-313 " disclosed polymer antimicrobial coating catheter, as glucamine-chitosan coat catheter, chitosan-polyvinyl alcohol hydrogel coating catheter, with novel antibacterial coating catheters such as organosilicon quaternary ammonium salt coating catheters.But these are antibacterial, antimicrobial coating catheter still also exists surface coated treatment complex process, may bring noxious substance into and affect the shortcomings such as health.
Summary of the invention
The object of this invention is to provide the preparation method of a kind of pharmaceutical antibacterial catheter of solving the problems of the technologies described above and this catheter, by using Antagonistic protein matter glue, making it form protein nano coating and be attached to catheter surface, and then obtained antibacterial catheter, thus use in vivo in this catheter process and effectively suppress the adhesion of various bacteria and mycete and grow.Object of the present invention is realized by following technical proposals:
The antibacterial catheter of a kind of albumen, have one deck non-dispersive nanoscale on the surface of catheter bulk material lubricious, the thickness of coating is 10nm ~ 500nm, and the r.m.s. roughness of coating is not more than 100nm; Described coating is processed to form through following steps: carry out pretreatment to catheter bulk material; Preparation protein glue; Use this protein glue, adopt one or more methods in infusion process, brushing method, rotary spraying method or ultrasonic spraying process, single or multiple coating is carried out to the surface of the catheter bulk material after process; And then one or more methods in employing spontaneous curing, microwave curing, heat cure, ultraviolet light polymerization or crosslinking curing, the protein glue being attached to catheter bulk material surface is cured, thus it is lubricious to form non-dispersive nanoscale.
Further, described native protein is one or more in serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin or lysozyme to the antibacterial catheter of albumen of the present invention.
Further, also containing non-protein class natural goods in the middle of the composition of described coating, described non-protein class natural goods is chitin, chitosan to the antibacterial catheter of albumen of the present invention, or their derivant.
Further, described catheter bulk material is latex, silicone rubber, polyurethane or polrvinyl chloride to the antibacterial catheter of albumen of the present invention.
The present invention also provides the preparation method of the antibacterial catheter of a kind of above-mentioned albumen, comprises the following steps:
Pretreatment is carried out to catheter bulk material; Preparation protein glue; Use one or more methods in the employing of this protein glue infusion process, brushing method, rotary spraying method or ultrasonic spraying process, single or multiple coating is carried out to the surface of the catheter bulk material after process; And then one or more methods in employing spontaneous curing, microwave curing, heat cure, ultraviolet light polymerization or crosslinking curing, the protein glue being attached to catheter bulk material surface is cured, thus it is lubricious to form non-dispersive nanoscale.
The preparation method of the antibacterial catheter of albumen of the present invention further, carrying out pretreated method to catheter bulk material is clean and/or modification, comprises one or more processing methods in washing, pickling, organic/inorganic solvent or solution washing, heat treatment, supersound process, Cement Composite Treated by Plasma, Modification of Photo-grafting Copolymerization, the modification of supramolecular chemistry surface adsorption.
The preparation method of the antibacterial catheter of albumen of the present invention further, the compound method of described protein glue is, initial glue is formed after being mixed with solvent by harmless native protein, add one or more in non-protein class natural materials, cross-linking agent, thickening agent or dispersant again as auxiliary element, secondary glue is formed after stirring, then described secondary glue is filtered, centrifugalize, removing bubble and solid particle after obtain clarify protein glue.
The preparation method of the antibacterial catheter of albumen of the present invention further, described native protein be serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin, lysozyme one or more; Described solvent is the aqueous solution of water or ethanol, ethylene glycol, glycerol, mannitol, sodium chloride, calcium chloride, sodium bicarbonate; Described non-protein class natural materials is chitin, chitosan or derivatives thereof, and its addition is 1% ~ 5% of initial glue gross weight; Described cross-linking agent is one or more combinations of carbodiimide, N-hydroxy-succinamide or genipin, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described thickening agent is one or more combinations of sodium carboxymethyl cellulose, xanthan gum or sodium alginate, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described dispersant is one or more combinations of sodium tripolyphosphate, PEG400 or polysorbate60, and its addition is 0.5% ~ 1.5% of initial glue gross weight.
The preparation method of the antibacterial catheter of albumen of the present invention further, adopt the method for heat cure to be cured by the protein glue being attached to catheter bulk material surface, condition of cure be temperature 75 DEG C ~ 180 DEG C, pressure 1MPa ~ 2MPa, hardening time 10min ~ 45min.
Technique effect of the present invention is mainly reflected in:
1. the antibacterial catheter containing protein nano coating provided by the invention, it is antibacterial, antibacterial effect is excellent, respond well during for preventing urinary tract infection caused by catheter, financial burden and physical pain that patient frequently changes catheter can be alleviated, and treatment duration can be reduced, economize on resources;
2. the present invention is attached to the coating of catheter surface is that nanoscale non-dispersive based on native protein is lubricious, this coating can be combined steadily in the long term with catheter matrix material, the pliability of catheter body is not almost affected, it also not easily dissociates or peels off, good stability in vivo, toxicity is low, can not to patient bring some malaise symptoms (as to the scratch of tissue or fray, residue is irritated), can ensure that product is used safely clinically;
3. antibacterial catheter provided by the invention is because containing protein nano coating, and its hydrophilic is better than former catheter surface, can show extraordinary lubricity in water environment, has both been convenient to doctor's operation, has felt comfortably cool when patient can be allowed again to use;
4. the preparation method of the antibacterial catheter of the present invention is simple, and raw material is easy to get, and the protein nano coating related to is not only applicable to the antibacterial, antibacterial of catheter, also can be applicable to other antibacterial product surface clinically.
Accompanying drawing explanation
Figure 1A is the 2D figure of embodiment 1 antibacterial catheter surface protein nano coating under atomic force microscope (AFM);
Figure 1B is the 3D figure of embodiment 1 antibacterial catheter surface protein nano coating under atomic force microscope (AFM);
Fig. 2 A is the 2D figure of Figure 1A embodiment 2 antibacterial catheter surface protein nano coating under atomic force microscope (AFM);
Fig. 2 B is the 3D figure of Figure 1A embodiment 2 antibacterial catheter surface protein nano coating under atomic force microscope (AFM);
Fig. 3 is embodiment 1 and embodiment 2 antibacterial catheter surface protein nano coating, and the water contact angle of the former catheter surface of contrast separately;
Fig. 4 is x-ray photoelectron power spectrum (XPS) figure of embodiment 1 and embodiment 2 antibacterial catheter surface protein nano coating;
Fig. 5 A is embodiment 1 antibacterial catheter biocidal property experiment microphotograph figure;
Fig. 5 B is contrast catheter biocidal property experiment microphotograph figure;
Fig. 6 is protein glue uv absorption spectra.
Detailed description of the invention
Below in conjunction with accompanying drawing and specific embodiment, the present invention will be described, for embodiment be only make generality to product of the present invention or method to illustrate, contribute to understanding the present invention better, but can't the scope of the invention be limited.Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all can obtain from commercial channels.
The antibacterial catheter of albumen provided by the invention, mainly have one deck non-dispersive nanoscale on the surface of catheter bulk material lubricious, the bulk composition of this coating is harmless native protein, the thickness of coating is 10nm ~ 500nm, and the r.m.s. roughness of coating is not more than 100nm.The thickness of this protein nano coating, generally between 10nm ~ 500nm, does not almost affect the pliability of catheter body; This protein nano coating r.m.s. roughness is handed over low, and surface is more smooth, and hydrophilic is better than the surface of former catheter raw material, and can show extraordinary lubricity in water environment, facilitate surgical operation, patient's use sense is comfortable.
Particularly, described native protein is one or more in serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin or lysozyme.The composition of above-mentioned coating also comprises non-protein class natural goods, particularly, described non-protein class natural goods is chitin, chitosan, or their derivant, and catheter bulk material of the present invention includes but not limited to latex, silicone rubber, polyurethane or polrvinyl chloride.Nanoscale due to catheter bulk material surface of the present invention is lubricious is non-dispersive coating, and be combined steadily in the long term with catheter substrate, not easily dissociate or peel off, in vivo good stability, toxicity is low, ensures product safe handling clinically.
Present invention also offers the preparation method of the antibacterial catheter of above-mentioned albumen, details are as follows for its step:
Pretreatment is carried out to catheter bulk material, this processing method carries out cleaning and/or modification, comprises one or more processing methods in washing, pickling, organic/inorganic solvent or solution washing, heat treatment, supersound process, Cement Composite Treated by Plasma, Modification of Photo-grafting Copolymerization, the modification of supramolecular chemistry surface adsorption;
Preparation protein glue, the compound method of this protein glue is, initial glue is formed after being mixed with solvent by harmless native protein, add one or more in non-protein class natural materials, cross-linking agent, thickening agent or dispersant again as auxiliary element, secondary glue is formed after stirring, then described secondary glue is filtered, centrifugalize, removing bubble and solid particle after obtain clarify protein glue;
Then this protein glue is used, adopt infusion process, brushing method, one or more methods in rotary spraying method or ultrasonic spraying process, single or multiple coating is carried out to the surface of the catheter bulk material after process, certainly, general knowledge as known to the skilled person, the tube cavity body inwall of catheter bulk material surface is not easy to adopt be coated with and brushes hair the coating carrying out protein glue, therefore above-mentioned brushing method is not comprised in the method for catheter tube cavity body intine-held protein matter glue-coating, but the pipe outer wall of catheter bulk material surface to adopt brushing method to carry out the coating of protein glue,
Finally adopt one or more methods in spontaneous curing, microwave curing, heat cure, ultraviolet light polymerization or crosslinking curing, the protein glue being attached to catheter bulk material surface is cured, thus it is lubricious to form non-dispersive nanoscale.The present invention preferably adopts the method for heat cure to be cured by protein glue, condition of cure be temperature 75 DEG C ~ 180 DEG C, pressure 1MPa ~ 2MPa, hardening time 10min ~ 45min.
In the preparation method of the antibacterial catheter of above-mentioned albumen further, described native protein be serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin, lysozyme one or more; Described solvent is the aqueous solution of water or ethanol, ethylene glycol, glycerol, mannitol, sodium chloride, calcium chloride, sodium bicarbonate; Described non-protein class natural materials is chitin, chitosan or derivatives thereof, and its addition is 1% ~ 5% of initial glue gross weight; Described cross-linking agent is one or more combinations of carbodiimide, N-hydroxy-succinamide or genipin, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described thickening agent is one or more combinations of sodium carboxymethyl cellulose, xanthan gum or sodium alginate, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described dispersant is one or more combinations of sodium tripolyphosphate, PEG400 or polysorbate60, and its addition is 0.5% ~ 1.5% of initial glue gross weight.
Antibacterial catheter containing protein nano coating provided by the invention, it is antibacterial, antibacterial effect is excellent, the urinary tract infection caused for preventing catheter respond well, alleviates financial burden and physical pain that patient frequently changes catheter, reduces treatment duration simultaneously, economizes on resources.And this protein nano coating hydrophilic is better than the surface of former catheter raw material, can show extraordinary lubricity in water environment, patient's use sense is comfortable.And this antibacterial catheter preparation method is simple, and raw material is easy to get, and the protein nano coating on it is not only applicable to the antibacterial, antibacterial of catheter, also can be applicable to other antibacterial product surface clinically.
[embodiment 1]
The antibacterial catheter of albumen, preparation process is as follows:
Catheter pretreatment: get complete silica fubber cathete raw material, with distilled water cyclic washing 5 times ~ 6 times, then it is immersed in completely in Piranha washing liquid (mixed solution of 98% concentrated sulphuric acid and 30% hydrogenperoxide steam generator 3:1 (v/v)), under 60 DEG C of water bath condition, processes 30min.
Prepared by protein glue: by lactalbumin, leucosin, lysozyme, the soluble powder of carboxymethyl chitosan presses certain mass ratio, ratio as 1.5:5:3:0.5 mixes, add in distilled water and obtain the initial glue that gross mass mark is 2wt% ~ 20wt%, then the PEG400 aqueous solution of 5mg/ml is added as dispersant according to the volume of albumen glue using the ratio of 50:1 (v/v), magnetic stirrer 1h is used to obtain secondary glue to solution, finally this secondary glue filters successively again, centrifugal, remove bubble and particle, obtain the protein glue clarified.
Protein glue applies: use the mode of ultrasonic spraying to carry out to the whole outer surface of pretreated silica fubber cathete the precision coating that single forms one deck.
The solidification of protein glue: under temperature 75 DEG C ~ 180 DEG C, pressure 1MPa ~ 2MPa condition, adopt heat cure mode to be cured obtained protein nano coating to the protein glue that catheter applies, hardening time is 10min ~ 45min.
[embodiment 2]
The antibacterial catheter of albumen, preparation process is as follows:
The pretreatment of catheter: get a complete latex material catheter, with the ethanol water cyclic washing 5 times ~ 6 times of 80% (v/v), naturally oxirane carries out 10min under 50 DEG C of conditions sterilization treatment to it is re-used after being dried by conduit, and then the low temperature plasma instrument putting into continuous radio frequency processes catheter surface, plasma discharge power and time are respectively 200W and 5min.
Prepared by protein glue: by fibroin albumen, lactalbumin, the soluble powder of leucosin and oralbumin presses certain mass ratio, ratio as 1:4:3:2 mixes, the ethylene glycol solution adding 20% (v/v) is the initial glue that solvent obtains that gross mass mark is 5wt% ~ 20wt%, then the sodium tripolyphosphate solution of 0.01mol/L is added as dispersant according to the volume of albumen glue using the ratio of 50:1 (v/v), magnetic stirrer 1h is used to obtain secondary glue to above-mentioned mixed solution, finally this secondary glue filters successively again, centrifugal, remove bubble and particle, obtain the protein glue clarified.
The coating of protein glue: use the mode of dipping to carry out adsorption-type coating to the whole outer surface of pretreated latex catheter, takes out after dipping 10min from Antagonistic protein matter glue.
The solidification of protein glue: when the catheter taken out from protein glue no longer includes under drop pouring, use microwave curing technology to be cured obtained protein nano coating catheter to Antagonistic protein matter glue, hardening time is 5min.
Antibacterial catheter prepared by above-described embodiment 1 and embodiment 2 two kinds of methods, its protein nano coating layer thickness is all in ten a few to tens of nanometer range, as depicted in figs. 1 and 2, the protein nano coating of embodiment 1 and the antibacterial catheter surface of embodiment 2 rises and falls little, r.m.s. roughness only has several nanometer (see table 1), and surface is smoother all.
The roughness of the antibacterial catheter surface protein nano coating of table 1 embodiment 1,2
Shown in Fig. 3 is the comparison of the water contact angle (WCA) of embodiment 1 and the antibacterial catheter of embodiment 2, and result shows: after the surface forming protein nano coating of the catheter of two kinds of different materials, contact angle all obviously reduces, and hydrophilic becomes better.Therefore, embodiment 1 and the antibacterial catheter of embodiment 2 have good lubricity, and facilitate the operation technique of doctor, patient also can feel comfortably cool.
Table 2 tests according to the x-ray photoelectron power spectrum of embodiment illustrated in fig. 41 and the antibacterial catheter surface of embodiment 2 the element molar content analysis obtained, result shows: the catheter of two kinds of different materials is after surface forming protein nano coating, N/C is than increasing severely, N element becomes essential element together with C, O, this is that typical protein is elementary composition, and other elements, the photoelectron signal as Si element becomes very faint, is clearly that the overwhelming majority has been masked by protein coating.Therefore, catheter overwhelming majority surface can be wrapped up very closely knit by the protein nano coating prepared by said method, and the protein used in the present invention is all native protein mostly, completely harmless to human body, the problems such as the zest that the surface of such protein coating also can avoid former catheter may bring to human body, safety in utilization is good.
The elemental composition analysis that the antibacterial catheter surface of table 2 embodiment 1,2 is corresponding
Fig. 5 is embodiment 1 antibacterial catheter biocidal property experiment microphotograph figure, and this experiment is 10 by the concentration resuspended through PBS buffer
7the escherichia coli bacteria suspension of cfu/ml drops in embodiment 1 antibacterial urinary catheter surface and former silicone rubber tube surface, in the incubator of 37 DEG C of conditions, cultivate 2h, cultivates and terminates the antibacterial catheter of rear PBS flushing embodiment 1, then at fluorescence microscopy Microscopic observation.Result shows: in 2h, the former silica fubber cathete not doing the process of protein nano coating is covered with E. coli clones, review the antibacterial catheter that embodiment 1 includes protein nano coating, all also cannot grow in its surface adhesion in antibacterial 2h, fungistatic effect clearly.
After more than experiment terminates, antibacterial for embodiment 1 catheter sample is put into corresponding culture medium solution as carrier cultivate, OD600 value (absorbance of 600nm wavelength light) in test different time sections, and set up " clump count-OD600 " standard curve with this.Such as, obtain antibacterial catheter in embodiment 1, standard curve equation may be: y=6.00E+9x, and therefore by the OD600 value of 41h, namely x=0.08 substitutes into equation, just can estimate that clump count is about 6.00E+0.72.Finally, the biocidal property of catheter is evaluated according to formula " bacteriostasis rate=(matched group bacterium number-test group bacterium number)/matched group bacterium number × 100% " and " bacterial adhesion rate=1-bacteriostasis rate ".As shown in table 3, with former silica fubber cathete for matched group, the 41h bacteriostasis rate of the antibacterial catheter of embodiment 1, close to 100%, take staphylococcus aureus as experimental strain, similar biocidal property evaluation is carried out to the antibacterial catheter of embodiment 1, its 41h bacteriostasis rate also nearly 100%.
The antibacterial catheter surface of table 3 embodiment 1,2 is as the biocidal property evaluation of carrier in 21h and 41h
Mixed with same ratio by the protein powder that being used in embodiment 1 prepares protein glue, be made into the glue of 0.1mg/ml with distilled water, measure the ultraviolet absorption curve of solution, as shown in Figure 6, there is characteristic absorption peak at 280nm place in curve.Above characteristic ultraviolet absorption can be used to the stability of analyzing proteins nano coating, method is soaked in certain density PBS solution by the antibacterial catheter sample containing protein nano coating, the ultraviolet absorption curve of solution is measured after certain hour, as occurred and crude protein glue characteristic of correspondence absworption peak, just to illustrate in this period that antibacterial supravasal coating there occurs and dissociating or peel off.Table 4 shows, and embodiment 1 and the antibacterial catheter of embodiment 2 have good stability, all dissociate in 240h, and its protein nano coating is all without diffusibility.
The stability analysis of the antibacterial catheter of two kinds of albumen nano coatings in table 4 embodiment 1 and 2
It should be noted that above-described embodiment is only used for explaining explanation, and should not be counted as the restriction to institute of the present invention content scope.Limit for fear of length, only describe comparatively typical implementation method in detail, should be fully recognized that the present invention can make related amendments for the innovative point and advantage not departing from its content purport for a person skilled in the art, and all this kind of amendments all should be included in that the present invention defines with context that is equivalent within.
Claims (9)
1. the antibacterial catheter of albumen, is characterized in that: have one deck non-dispersive nanoscale on the surface of catheter bulk material lubricious, and the thickness of coating is 10nm ~ 500nm, and the r.m.s. roughness of coating is not more than 100nm; Described coating is processed to form through following steps,
Pretreatment is carried out to catheter bulk material; Preparation protein glue; Use this protein glue, adopt one or more methods in infusion process, brushing method, rotary spraying method or ultrasonic spraying process, single or multiple coating is carried out to the surface of the catheter bulk material after process; And then one or more methods in employing spontaneous curing, microwave curing, heat cure, ultraviolet light polymerization or crosslinking curing, the protein glue being attached to catheter bulk material surface is cured, thus it is lubricious to form non-dispersive nanoscale.
2. the antibacterial catheter of albumen according to claim 1, is characterized in that: described native protein is one or more in serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin or lysozyme.
3. the antibacterial catheter of albumen according to claim 1 and 2, is characterized in that: also containing non-protein class natural goods in the middle of the composition of described coating.
4. the antibacterial catheter of albumen according to claim 3, is characterized in that: described non-protein class natural goods is chitin, chitosan, or their derivant.
5. the antibacterial catheter of albumen according to claim 1, is characterized in that: described catheter bulk material is latex, silicone rubber, polyurethane or polrvinyl chloride.
6. the preparation method of the antibacterial catheter of albumen according to claim 1, it is characterized in that: carrying out pretreated method to catheter bulk material is clean and/or modification, comprise one or more processing methods in washing, pickling, organic/inorganic solvent or solution washing, heat treatment, supersound process, Cement Composite Treated by Plasma, Modification of Photo-grafting Copolymerization, the modification of supramolecular chemistry surface adsorption.
7. the preparation method of the antibacterial catheter of albumen according to claim 1, it is characterized in that: the compound method of described protein glue is, initial glue is formed after being mixed with solvent by harmless native protein, add one or more in non-protein class natural materials, cross-linking agent, thickening agent or dispersant again as auxiliary element, secondary glue is formed after stirring, then described secondary glue is filtered, centrifugalize, removing bubble and solid particle after obtain clarify protein glue.
8. the preparation method of the antibacterial catheter of albumen according to claim 7, is characterized in that:
Described native protein be serum albumin, fibroin albumen, lactalbumin, collagen protein, leucosin, oralbumin, large legumelin, lysozyme one or more; Described solvent is the aqueous solution of water or ethanol, ethylene glycol, glycerol, mannitol, sodium chloride, calcium chloride, sodium bicarbonate; Described non-protein class natural materials is chitin, chitosan or derivatives thereof, and its addition is 1% ~ 5% of initial glue gross weight; Described cross-linking agent is one or more combinations of carbodiimide, N-hydroxy-succinamide or genipin, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described thickening agent is one or more combinations of sodium carboxymethyl cellulose, xanthan gum or sodium alginate, and its addition is 0.5% ~ 1.5% of initial glue gross weight; Described dispersant is one or more combinations of sodium tripolyphosphate, PEG400 or polysorbate60, and its addition is 0.5% ~ 1.5% of initial glue gross weight.
9. the preparation method of the antibacterial catheter of albumen according to claim 1, it is characterized in that: adopt the method for heat cure to be cured by the protein glue being attached to catheter bulk material surface, condition of cure be temperature 75 DEG C ~ 180 DEG C, pressure 1MPa ~ 2MPa, hardening time 10min ~ 45min.
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| CN114681685B (en) * | 2022-04-08 | 2023-05-19 | 东莞市人民医院 | Preparation method of hybrid protein coating, hybrid protein coating material and application |
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| CN103160786A (en) * | 2013-03-07 | 2013-06-19 | 苏州睿研纳米医学科技有限公司 | Nano coating preparation method and antibiosis nano coating prepared by nano coating |
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| WO2018214087A1 (en) * | 2017-05-25 | 2018-11-29 | 苏州睿研纳米医学科技有限公司 | Anti-biological, extracellular matrix adhesion coating and preparation method therefor and use thereof |
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