CN103768611B - Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) C1 nano anti-cancer medicine and preparation method thereof - Google Patents
Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) C1 nano anti-cancer medicine and preparation method thereof Download PDFInfo
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- CN103768611B CN103768611B CN201210428054.9A CN201210428054A CN103768611B CN 103768611 B CN103768611 B CN 103768611B CN 201210428054 A CN201210428054 A CN 201210428054A CN 103768611 B CN103768611 B CN 103768611B
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Abstract
The present invention relates to a kind of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine and preparation method thereof.Phytogenous alcohol-soluble protein was mainly first chelated in ethanol water by its scheme with zinc salt, and regulating pH is 7.0～10.0,10～50 DEG C of constant temperature standing and reacting 1～10 days.Centrifugal enrichment precipitates.Again precipitation is dispersed in the ethanol water containing cadmium salt, 10～50 DEG C of constant temperature standing and reacting 1～10 days.Centrifugal enrichment precipitates, and lyophilization prepares Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of white nano bar-shape.The method mild condition, simple to operate, Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine prepared has the activity of excellent anticancer growth, can be applied to the fields such as neural cancer, hepatocarcinoma and skin carcinoma as new type anticancer medicine.
Description
Technical field
The present invention relates to biochemical drug technical field, in more detail a kind of Phytogenous alcohol-soluble protein conjugation ZnO/Cd
(OH) Cl nano anti-cancer medicine and preparation method thereof.
Background technology
Cancer is one of disease of serious harm human health, has higher M & M.Use traditional
Cancer therapy drug carries out chemotherapy to patient can treat cancer to a certain extent, but, easy due to cancerous cell over the course for the treatment of
Produce drug resistance and cause chemotherapy to lose efficacy.For avoiding or reducing these untoward reaction, there has been proposed many schemes, including associating
Chinese medicine is treated, but there is no effective solution at present.
The inhibitory action of cancerous cell is in the news by metal ion the most successively.Such as zinc salt and cancerous cell effect
After, cancerous cell can be induced to produce response to oxidative stress, ultimately result in cancer cell death.But, owing to metal ion is at water solublity
Environment is prone to diffusion so that it is be difficult to effectively act on cancerous cell.Along with the development of nanotechnology, metal ion is prepared as
Insoluble solid nano-particle containing this metal ion, can overcome above-mentioned difficulties.
Summary of the invention
The problem to be solved in the present invention is: provide a kind of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer
Medicine.
The invention solves the problems that another problem is that offer above-mentioned Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nanometer
The preparation method of cancer therapy drug.
Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine that the present invention provides, it is characterised in that:
This Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine is by Phytogenous alcohol-soluble protein, ZnO and Cd
(OH) nanometer rods of Cl composition.
The preparation method of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine that the present invention provides, its
It is characterised by: the method is that Phytogenous alcohol-soluble protein is first chelated in the mixed solution of second alcohol and water with zinc salt.With
Rear regulation pH is 7.0～10.0, constant temperature standing and reacting 1～10 days.After gained pelleting centrifugation is enriched with, again it is dispersed in containing cadmium
In the mixed solution of the second alcohol and water of salt, constant temperature standing and reacting 1～10 days.Finally, being enriched with by pelleting centrifugation, lyophilization prepares
Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.
In described preparation method, the Phytogenous alcohol-soluble protein of use includes Semen Maydis protein,alcohol-soluble, Semen Tritici aestivi alcohol-soluble
Albumen, Fructus Hordei Vulgaris protein,alcohol-soluble and Herba bromi japonici protein,alcohol-soluble.Described zinc salt is zinc nitrate, zinc chloride.Described cadmium salt is nitre
Acid cadmium, Caddy (Cleary).
The Phytogenous alcohol-soluble protein concentration used in reactant is 0.1～20mg/mL, and zinc salt concentration is 0.1～40mg/
ML, cadmium salt concentration is 0.15～40mg/mL, and in the mixed solution of second alcohol and water, the ratio of second alcohol and water is 19: 1～3: 2, instead
Answering temperature is 10～50 DEG C.
Advantages of the present invention:
1, Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine that the present invention provides, its preparation method
Reaction temperature controls at 10～50 DEG C, mild condition, and the solvent of use includes water and ethanol, environmental friendliness.
2, reaction need not special equipment, it is not necessary to follow-up complicated purification step, easy to operate, and product is dispersion
Good Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine, is suitable to extensive industry and produces, have wide
General application prospect.
3, Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine that the present invention provides is thin to neural cancer
Born of the same parents (PC12), hepatoma carcinoma cell (BEL-7402) and dermal melanin oncocyte (B16-F10) growth have excellent inhibitory action.
Accompanying drawing explanation
The infrared spectrum of zein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of Fig. 1: embodiment 1 preparation.
The X diffracting spectrum of zein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of Fig. 2: embodiment 1 preparation.
The transmission electron microscope of zein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of Fig. 3: embodiment 1 preparation shines
Sheet.
The transmission electron microscope of zein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of Fig. 4: embodiment 2 preparation shines
Sheet.
The transmission electron microscope of wheat gliadin conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of Fig. 5: embodiment 3 preparation shines
Sheet.
Phytogenous alcohol-soluble protein prepared by Fig. 6: the present invention is conjugated ZnO/Cd (OH) Cl nano anti-cancer medicine and Corning
The suppression (1) of Tissue Culture Plate (PLASTIC): neural cancerous cell (PC12)；(2): hepatoma carcinoma cell (BEL-7402) and (3): skin
Skin melanoma cell (B16-F10) compares (cancerous cell and Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer
The medicine effect quantity of 2 days).(a): zein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine；(b): Semen Tritici aestivi alcohol is molten
Albumen conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine；(c): hordein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine
Thing；(d): Herba bromi japonici alcohol soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.
Detailed description of the invention
Be will assist in by following embodiment and understand the present invention, but be not limiting as present disclosure.
Embodiment 1: prepared by zein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine
Measure zein ethanol water (v/v=3: the 2) 40mL that concentration is 0.2mg/mL, and concentration the most respectively
For zinc chloride ethanol water (v/v=3: the 2) 40mL of 0.2mg/mL, the two is mixed.With the ethanol water of sodium hydroxide
(v/v=3: 2) regulation pH was 7.0,10 DEG C of constant temperature standing and reacting 10 days.10,000 revs/min of centrifugal enrichment precipitations.
The most again precipitation is dispersed in the ethanol water (v/v=3: 2) that 40mL contains 0.15mg/mL Caddy (Cleary),
10 DEG C of constant temperature standing and reacting 10 days.10,000 revs/min of centrifugal enrichment precipitations, lyophilization prepares the Semen Maydis of white nano bar-shape
Alcohol soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.The infrared spectrum of product is as it is shown in figure 1, X diffracting spectrum such as Fig. 2 institute
Showing, transmission electron microscope photo is as shown in Figure 3.
Embodiment 2: prepared by zein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine
Measure zein ethanol water (v/v=19: the 1) 40mL that concentration is 40mg/mL, and concentration the most respectively
For zinc nitrate ethanol water (v/v=19: the 1) 40mL of 80mg/mL, the two is mixed.With the alcohol-water solution (v/ of sodium hydroxide
V=19: 1) regulation pH was 10.0,50 DEG C of constant temperature standing and reacting 1 day.10,000 revs/min of centrifugal enrichment precipitations.
The most again precipitation is dispersed in the ethanol water (v/v=19: 1) that 40mL contains 40mg/mL cadmium nitrate, 50
DEG C constant temperature standing and reacting 1 day.10,000 revs/min of centrifugal enrichment precipitations, lyophilization prepares the Semen Maydis alcohol of white nano bar-shape
Molten albumen conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.The transmission electron microscope photo of product is as shown in Figure 4.
Embodiment 3: prepared by wheat gliadin conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine
Measure wheat gliadin ethanol water (v/v=17: the 3) 40mL that concentration is 20mg/mL, and concentration the most respectively
For zinc nitrate ethanol water (v/v=17: the 3) 40mL of 40mg/mL, the two is mixed.With the alcohol-water solution (v/ of sodium hydroxide
V=17: 3) regulation pH was 8.5,30 DEG C of constant temperature standing and reacting 5 days.10,000 revs/min of centrifugal enrichment precipitations.
The most again precipitation is dispersed in the ethanol water (v/v=17: 3) that 40mL contains 20mg/mL cadmium nitrate, 30
DEG C constant temperature standing and reacting 5 days.10,000 revs/min of centrifugal enrichment precipitations, lyophilization prepares the Semen Tritici aestivi alcohol of white nano bar-shape
Molten albumen conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.The transmission electron microscope photo of product is as shown in Figure 5.
Embodiment 4: the antitumor cell curative effect of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine
Choosing neural cancerous cell (PC12), hepatoma carcinoma cell (BEL-7402) and dermal melanin oncocyte (B16-F10) is
Model, the antitumor curative effect of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine is by hatching altogether with cell
After cell survival rate evaluate.Cell survival rate experiment uses determination of cell count.Train in advance in 96 hole Corning culture plates
Support 12 hour cells, be separately added into Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine of 5mg/L, and set
Control wells, often group is repeated 6 times；After hatching 2 days, counted under microscope, result is as shown in Figure 6.
Claims (5)
1. Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine, is characterized in that: this plant-derived alcohol
Molten albumen conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine is to neural cancerous cell, hepatoma carcinoma cell and the growth of dermal melanin oncocyte
Inhibited, this Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine is by the molten egg of plant-derived alcohol
In vain, the nanometer rods of ZnO and Cd (OH) Cl composition.
2. Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine as claimed in claim 1, is characterized in that:
Described Phytogenous alcohol-soluble protein is Semen Maydis protein,alcohol-soluble, Semen Tritici aestivi protein,alcohol-soluble, Fructus Hordei Vulgaris protein,alcohol-soluble, Herba bromi japonici alcohol-soluble
Albumen.
3. Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine as claimed in claim 1, is characterized in that:
Described Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine is white nano bar-shape.
4. the system of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) the Cl nano anti-cancer medicine as described in claim 1 or 2 or 3
Preparation Method, is characterized in that: its step is as follows:
1) 0.1～20mg/mL Phytogenous alcohol-soluble protein and 0.1～40mg/mL zinc salt are first carried out chela in ethanol water
Closing, described zinc salt is zinc chloride, and regulation pH is 7.0～10.0,10～50 DEG C of constant temperature standing and reacting 1～10 days, and centrifugal enrichment
Precipitation；
2) again precipitation being dispersed in the ethanol water that 40mL contains 0.15～40mg/mL cadmium salt, described cadmium salt is chlorine
Cadmium, 10～50 DEG C of constant temperature standing and reacting 1～10 days, centrifugal enrichment precipitated, and lyophilization prepares Phytogenous alcohol-soluble protein
Conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine.
5. the preparation side of Phytogenous alcohol-soluble protein conjugation ZnO/Cd (OH) Cl nano anti-cancer medicine as claimed in claim 4
Method, is characterized in that: the ratio of described second alcohol and water is 19: 1～3: 2.
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| CN113716601A (en) * | 2021-09-08 | 2021-11-30 | 吉林大学 | Hydroxyl cadmium chloride crystal and preparation method thereof |
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| CN101125150A (en) * | 2007-08-13 | 2008-02-20 | 王小丁 | Application of nanometer zinc oxide in preparing medicine for treating malignant tumor |
| CN101165469A (en) * | 2006-10-19 | 2008-04-23 | 河南师范大学 | Protein coated silver sulfide nano line preparation method |
| CN101358973A (en) * | 2007-08-02 | 2009-02-04 | 四川大学 | Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array |
| CN101518546A (en) * | 2009-03-25 | 2009-09-02 | 吉林大学第一医院 | Application of combination of cadmium chloride and zinc chloride in preparation of anti-tumor drugs |
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| AUPN442295A0 (en) * | 1995-07-26 | 1995-08-17 | Commonwealth Scientific And Industrial Research Organisation | Regulated autocrine growth of mammalian cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101165469A (en) * | 2006-10-19 | 2008-04-23 | 河南师范大学 | Protein coated silver sulfide nano line preparation method |
| CN101358973A (en) * | 2007-08-02 | 2009-02-04 | 四川大学 | Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array |
| CN101125150A (en) * | 2007-08-13 | 2008-02-20 | 王小丁 | Application of nanometer zinc oxide in preparing medicine for treating malignant tumor |
| CN101518546A (en) * | 2009-03-25 | 2009-09-02 | 吉林大学第一医院 | Application of combination of cadmium chloride and zinc chloride in preparation of anti-tumor drugs |
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