CN103766845A - Preparation method of bamboo fungi meat-flavored essence - Google Patents

Preparation method of bamboo fungi meat-flavored essence Download PDF

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Publication number
CN103766845A
CN103766845A CN201410005898.1A CN201410005898A CN103766845A CN 103766845 A CN103766845 A CN 103766845A CN 201410005898 A CN201410005898 A CN 201410005898A CN 103766845 A CN103766845 A CN 103766845A
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China
Prior art keywords
meat
dictyophora phalloidea
preparation
flavor
water
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CN201410005898.1A
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Chinese (zh)
Inventor
黄明泉
孙宝国
田红玉
郑福平
刘玉平
陈海涛
谢建春
张玉玉
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Beijing Technology and Business University
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Beijing Technology and Business University
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Priority to CN201410005898.1A priority Critical patent/CN103766845A/en
Publication of CN103766845A publication Critical patent/CN103766845A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/201Compounds of unspecified constitution characterised by the chemical reaction for their preparation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/21Synthetic spices, flavouring agents or condiments containing amino acids
    • A23L27/22Synthetic spices, flavouring agents or condiments containing amino acids containing glutamic acids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/26Meat flavours
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Seasonings (AREA)

Abstract

The invention provides a preparation method of bamboo fungi meat-flavored essence and belongs to the technical field of food processing. The preparation method comprises the following two steps: firstly, mixing the fresh meat paste and water with the same quantity, adding a certain quantity of flavored enzymes and pancreatic enzymes, enzymatically hydrolyzing for 0.25-0.45 hour under the conditions that the pH value of a solution is 5.5-7.5 and the temperature is 50-55 DEG C; secondly, in percentage by mass, reacting a certain quantity of enzymatically hydrolyzed substances (accounting for 45%-55% of reactants) with materials such as 0.1%-0.6% of bamboo fungi, 7%-12% of HVP (hydrolyzed vegetable protein) liquid, 1.0%-1.5% of grease and 0%-1.5% of ammonium sulfide for 2-6 hours under the conditions that the pH value is 5.5-7.5 and the temperature is 100-120 DEG C, cooling and homogenizing to obtain the bamboo fungi meat-flavored essence. The bamboo fungi meat-flavored essence is rich in meat flavor, long in flavor retention time and low in chloropropanol content, and can be directly used in foods such as meat products and instant noodles.

Description

A kind of preparation method of dictyophora phalloidea meat flavor
Technical field
The present invention designs a kind of preparation method of dictyophora phalloidea thermal processing meat flavour, belongs to food processing technology field.Products obtained therefrom meat is aromatic strongly fragrant, and fragrance remaining time is long, and chloropropyl alcohol content is low, safer, can directly in the food such as meat products, instant noodles, use.
Technical background
Thermal processing meat flavour (abbreviation meat flavor) is the class novel foodstuff essence that last century, the seventies rose, and in the food such as meat products, Flour product, quick-frozen food, dilated food, bakery product, compound seasoner, plant protein preparation, application is very general.
Meat flavor manufacture method is mainly that blending combines with thermal response.Blending spices used comprises natural perfume material and the large class of synthetic perfume two.The basis of thermal response is Maillard reaction, and its primary raw material is amino acid, reduced sugar and other batchings.Hydrolyzed vegetable protein (Hydrolized Vegetable Protein, HVP), HAP (HAP), yeast etc. are main amino acid sources.
Generally, meat flavor is safe, but due to the difference of raw material sources and production technology, may have trace toxic harmful substance, such as chloropropyl alcohol (3-MCPD) in fraction meat flavor.
By the investigation discovery of pertinent literature, chloropropyl alcohol is being permitted numerous food [1-4]in one of the harmful substance that all exists, what wherein content was more is acid hydrolyzed vegetable protein liquid (HVP), soy sauce, and part hot-working product.
Chloropropyl alcohol refers to the class chemical substance that chemical composition is similar, comprises 3-chlorine-1,2-propylene glycol (3-MCPD), 1, the chloro-2-propyl alcohol of 3-bis-(1,3-DCP), 2,3-bis-trimethylewne chlorohydrin 3-s (2,3-DCP) with the chloro-1,3-PD of 2-(2-MCPD).The chloropropyl alcohol of different molecular structures, its toxicity difference, is mainly that the toxicity of 3-MCPD and 1,3-DCP is large, chloropropyl alcohol is to all toxic effects such as liver, kidney, nerve, hematological system, reproductive systems, but also can be carcinogenic [1,5-7].But for above-mentioned several chloropropyl alcohols, the chloropropyl alcohol being present in relatively in a large number in food is mainly 3-chlorine-1,2-propylene glycol (3-MCPD), so in view of its harmfulness, many countries have formulated limit standard and control the pollution of chloropropyl alcohol in food.Most countries is to be less than 1.0mg/kg for the limitation of 3-MCPD, and the limitation of European Union is 20 μ g/kg.
In food, the forming process of chloropropyl alcohol mainly contains two aspects: the one, and the hydrolysis by raw oil material compounds in process or catabolite, react with chloride and generate, and this is the main path that chloropropyl alcohol forms; The 2nd, because exogenous pollution causes, as the raw material self of manufacturing food contains chloropropyl alcohol, or food is contained the packaging material pollution of chloropropyl alcohol and is produced in storage.
Based on the formation approach of chloropropyl alcohol in above-mentioned food, consider that the preparation process of meat flavor and the process of numerous food product have similitude, also confirmed by experiment to contain in meat flavor chloropropyl alcohol simultaneously, its source mainly contains two: the thermal response raw material that (1) contains chloropropyl alcohol is introduced, particularly HVP liquid; (2) hydrolysis or catabolite and the chloride reaction generation of raw oil material compounds in thermal process reactor.Grease class raw material comprises lard, butter, chicken fat etc., and chloride mainly contains sodium chloride, cysteine hydrochloride.Now at present general meat flavor, the consumption of sodium chloride can be up to 10% left and right, and the object of doing is like this mainly anticorrosion, postpones its shelf-life.But like this because the high-load of sodium chloride has also caused the high-load of chloropropyl alcohol in meat flavor, if meat flavor chloropropyl alcohol (3-MCPD) content having by market survey is up to 0.50mg/kg.
In order to solve the high problem of chloropropyl alcohol in meat flavor (3-MCPD) content, make it safer, by reduce the method for sodium chloride consumption in thermal response formula, (content declines the application, antisepsis weakens), optimize thermal rection condition and reduce chloropropyl alcohol content in meat flavor.Reduce in order to make up sodium chloride consumption the decline that causes meat flavor antiseptic property simultaneously, strengthen antiseptic property by adding a certain amount of dictyophora phalloidea raw material (thering is antisepsis, natural antiseptic agent).In addition, due to dictyophora phalloidea cost of material height can cause meat flavor cost increase, so by thermal response formula, add ammonium sulfide (price cheapness) as " sulphur source " replace conventional cysteine (price is very high) thus lower its cost.In the dictyophora phalloidea meat flavor making like this, the content of chloropropyl alcohol (3-MCPD) is very low, and cost is low, and meat is aromatic strongly fragrant simultaneously, and lasting is lasting.This essence and preparation method thereof has no report in existing technology.
Summary of the invention
Technical problem
The object of this invention is to provide a kind of method that adopts low content chlorination sodium thermal response to fill a prescription to prepare meat flavor.This method has overcome existing methodical shortcoming and defect.Adopt the content of chloropropyl alcohol (3-MCPD) in dictyophora phalloidea meat flavor prepared by this technique very low, cost is low, and meat is aromatic strongly fragrant simultaneously, and lasting is lasting.This essence can directly use in the food such as meat products, instant noodles.
Technical scheme
The technical solution adopted in the present invention is as follows: first step enzyme digestion reaction, get a certain amount of fresh meat meat gruel and etc. the water of quality, be uniformly mixed, then add food flavor enzyme (0~0.40%, account for the mass percent of meat, lower with) and pancreatin (0~0.15%), be 5.5~7.5 at pH value of solution, temperature is under the conditions such as 50 ℃~55 ℃, enzymolysis 0.25h~0.45h; Second step thermal response, get a certain amount of zymolyte (45%~55%, account for the mass percent of reactant, lower same), add respectively dictyophora phalloidea 0.1%~0.6%, hydrolyzed vegetable protein liquid (HVP) 7%~12%, grease 1.0%~1.5%, cysteine 0~0.8%, ammonium sulfide 0~1.5%, glucose 0.5%~1.2%, wood sugar 0.25%~0.65%, ossein 4%~7%, yeast extract 1%~3%, sodium chloride 4%~7%, glycine 0.14g0.3%~0.5%, glutamic acid 0.1%~0.3%, alanine 0.1%~0.3%, vitamin V B 10.05%~0.15%, spice 0.1%~0.3%, and suitable quantity of water, be then 5.5~7.5 at pH, under 100 ℃~120 ℃ conditions of temperature, and reaction 2~6h obtains dictyophora phalloidea meat flavor after cooling, homogeneous.
Meat gruel involved in the present invention is the one of the edibility meats such as pork, chicken, mutton, or its combination.
Dictyophora phalloidea involved in the present invention is Cultivation of Dictyophora, short-skirted veiled lady, Dictyophora echino-volvata Zane, Dictyophora rubrovalvata, or the combination of above-mentioned various dictyophora phalloidea.The consumption of dictyophora phalloidea in thermal response formula is 0.1%~0.6%.
Water involved in the present invention is that running water, mineral water, distilled water, deionized water or mass concentration are 0.5%~10% sodium chloride solution.
Grease involved in the present invention is the one in lard, chicken fat, sheep wet goods animal fat, or its combination, and the consumption in formula is 1.0%~1.5%.
Ossein involved in the present invention is one or its combination in pig ossein, chicken ossein, sheep ossein, and the consumption in formula is 4%~7%.
Spice involved in the present invention is the one in capsicum, Chinese prickly ash, cassia bark, anise, cumin, dried orange peel, spiceleaf, cloves etc., or its combination, and the consumption in formula is 0.1%~0.3%.
Hydrolyzed vegetable protein liquid (HVP), yeast extract, cysteine, ammonium sulfide, glucose, wood sugar, sodium chloride, glycine, glutamic acid, alanine, Cobastab involved in the present invention 1be food grade Deng reagent, the consumption in formula is shown in technique scheme.
Reaction temperature of the present invention, first step enzyme digestion reaction temperature is with the temperature between 50 ℃~55 ℃ for well, and the temperature of second step thermal response is better with the temperature between 100 ℃~120 ℃.
PH value of solution in two-step reaction of the present invention is relatively good between 5.5~7.5.
The preparation method of meat flavor of the present invention, can effectively reduce the chloropropyl alcohol content in meat flavor, and meat is aromatic strongly fragrant simultaneously, and feature is obvious, and lasting is lasting.This essence can directly use in the food such as quick-freezing boiled dumplings, meat products, instant noodles.
The specific embodiment:
The preparation of embodiment 1 pork zymolyte
The meat gruel 50g of fresh lean and 50g deionized water are added in 250mL four-hole boiling flask, after mixing, be positioned in 55 ℃ of water-baths, start mixer and stir, regulate pH to 6.7-7.0 with phosphate buffer solution simultaneously, add food flavor enzyme 0.150g and pancreatin 0.050g, enzymolysis 0.5h, and then pork zymolyte is boiled to 15min, and be cooled to room temperature, obtain the pork zymolyte of 1: 1.
The preparation of embodiment 2 dictyophora phalloidea pork essences 1
Toward adding following reaction raw materials in heavy wall pressure pipe: Cultivation of Dictyophora 0.165g, pork zymolyte 16.5g, HVP3.3g (10%), lard 0.45g, cysteine 0.20g, glucose 0.30g, wood sugar 0.165g, pig ossein 2.0g, yeast extract 0.7g, sodium chloride 2.0g, glycine 0.16g, glutamic acid 0.08g, alanine 0.08g, VB 10.045g, compound material (formulated according to a certain percentage by cloves, nutmeg, Chinese cassia tree, anise, Chinese prickly ash, onion, ginger) 0.8g.With phosphate buffer solution adjusting pH to 6.7-7.0, then supplementing deionized water to the quality of system is 40.00g.In reaction tube, add miniature magnetic stirrer, the heavy wall pressure pipe that installs reactant is placed in to 100 ℃ of constant temperature pots and reacts one section of 2h, cool, obtain dictyophora phalloidea pork essence.3-MCPD in this meat flavor is detected to analysis, and the content of known 3-MCPD is 4.13 μ g/kg, far below relevant limit standard.Visible, very safe by the meat flavor of this formula system, simultaneously through sensory evaluation, meat flavour is obvious, and feature is outstanding.
The preparation of embodiment 3 dictyophora phalloidea pork essences 2
Toward adding following reaction raw materials in heavy wall pressure pipe: Cultivation of Dictyophora 0.165g, pork zymolyte 16.5g, HVP3.4g, lard 0.5g, ammonium sulfide 0.40g, glucose 0.40g, wood sugar 0.165g, pig ossein 2.0g, yeast extract 0.8g, sodium chloride 2.0g, glycine 0.15g, glutamic acid 0.08g, alanine 0.08g, VB 10.040g, compound material (formulated according to a certain percentage by cloves, nutmeg, Chinese cassia tree, anise, Chinese prickly ash, onion, ginger) 0.8g, last adding distil water to the quality of total system is 40.0g.In reaction tube, add miniature magnetic stirrer, the heavy wall pressure pipe that installs reactant is placed in to 100 ℃ of constant temperature pots and reacts one section of 2h, cool, obtain dictyophora phalloidea pork essence.3-MCPD in pork essence is detected, and its content is 3.99 μ g/kg, far below relevant limit standard.Visible, very safe by the meat flavor of this formula system, simultaneously through sensory evaluation, meat flavour is obvious, and feature is outstanding.

Claims (3)

1. the preparation method of a dictyophora phalloidea meat flavor, it is characterized in that take meat, dictyophora phalloidea etc. as raw material, formed by enzyme digestion reaction and thermal response two step process: the first step, get a certain amount of fresh meat meat gruel and etc. the water of quality, be uniformly mixed, then add food flavor enzyme (0~0.40%, account for meat quality percentage, lower with) and pancreatin (0~0.15%), be 5.5~7.5 at pH value of solution, temperature is under the conditions such as 50 ℃~55 ℃, enzymolysis 0.25h~0.45h; Second step thermal response, get a certain amount of zymolyte (45%~55%, account for the mass percent of instead always answering thing, lower same), add respectively dictyophora phalloidea 0.1%~0.6%, hydrolyzed vegetable protein (HVP) liquid 7%~12%, grease 1.0%~1.5%, cysteine 0~0.8%, ammonium sulfide 0~1.5%, glucose 0.5%~1.2%, wood sugar 0.25%~0.65%, ossein 4%~7%, yeast extract 1%~3%, sodium chloride 4%~7%, glycine 0.14g0.3%~0.5%, glutamic acid 0.1%~0.3%, alanine 0.1%~0.3%, vitamin V B 10.05%~0.15%, spice 0.1%~0.3%, and suitable quantity of water, be then 5.5~7.5 at pH, under 100 ℃~120 ℃ conditions of temperature, reaction 2~6h.
2. the preparation method of a kind of dictyophora phalloidea meat flavor according to claim 1, it is characterized in that the formula of second step thermal response: meat zymolyte (45%~55%, account for the mass percent of reactant, lower same), dictyophora phalloidea 0.1%~0.6%, hydrolyzed vegetable protein (HVP) liquid 7%~12%, grease 1.0%~1.5%, cysteine 0~0.8%, ammonium sulfide 0~1.5%, glucose 0.5%~1.2%, wood sugar 0.25%~0.65%, ossein 4%~7%, yeast extract 1%~3%, sodium chloride 4%~7%, glycine 0.14g0.3%~0.5%, glutamic acid 0.1%~0.3%, alanine 0.1%~0.3%, VB 10.05%~0.15%, spice 0.1%~0.3%, and suitable quantity of water.
3. the preparation method of a kind of dictyophora phalloidea meat flavor according to claim 1, is characterized in that the noxious material chloropropyl alcohol≤20 μ g/kg in dictyophora phalloidea meat flavor.
CN201410005898.1A 2014-01-07 2014-01-07 Preparation method of bamboo fungi meat-flavored essence Pending CN103766845A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109430803A (en) * 2018-12-20 2019-03-08 天津春发生物科技集团有限公司 A kind of dictyophora phalloidea salt taste essence and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101361555A (en) * 2007-08-07 2009-02-11 安琪酵母股份有限公司 Beef flavouring matter and production method thereof
CN101390602A (en) * 2008-11-05 2009-03-25 天津市双星香精香料有限公司 Preparation method of natural meat flavor essence

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101361555A (en) * 2007-08-07 2009-02-11 安琪酵母股份有限公司 Beef flavouring matter and production method thereof
CN101390602A (en) * 2008-11-05 2009-03-25 天津市双星香精香料有限公司 Preparation method of natural meat flavor essence

Non-Patent Citations (1)

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Title
黄明泉: "热反应肉味香精中氯丙醇的形成与新产品开发研究", 《中国博士学位论文全文数据库 工程科技I辑》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109430803A (en) * 2018-12-20 2019-03-08 天津春发生物科技集团有限公司 A kind of dictyophora phalloidea salt taste essence and preparation method thereof

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