CN103761771B - Three-dimensional visual model of main fiber composition mesh structure in human skin - Google Patents
Three-dimensional visual model of main fiber composition mesh structure in human skin Download PDFInfo
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Abstract
The invention belongs to the technical field of histological anatomy, and particularly relates to a three-dimensional visual model of a main fiber composition mesh structure in the human skin. According to the technical scheme, the three-dimensional visual model of the main fiber composition mesh structure in the human skin is obtained through the method which comprises the steps of a, conducting histologic sectioning, b, conducting dying, c, collecting an image, d, conducting image registration, e, conducting image binaryzation processing, and f, conducting three-dimensional reconstruction visualization. The model can be used for three-dimensional printing of the main fiber composition mesh structure in the human skin, biomimetic material preparation, research for the skin physiological function and the like.
Description
Technical field
The invention belongs to technical field of histological anatomy, and in particular in application on human skin, majority fibers composition mesh structure is three-dimensional
Visual model.
Background technology
Reconstruction of medical images technology be computer graphicss and image procossing in biomedical engineering it is important should
With.Human tissue organ is carried out in medical diagnostics field, computed tomography (CT), NMR (Nuclear Magnetic Resonance)-imaging (MRI) etc.
After three-dimensional reconstruction, the three-dimensional intuitively 3D images of doctor can be presented to, is easy to the diagnosis state of an illness, is determined therapeutic scheme；Giving birth to
Thing medical domain, various novel three-dimensional reconstruction techniques and instrument are continued to bring out, such as pico computer layer scanning technology (Micro-
CT), laser scanning co-focusing microscope (CLSM) etc..
But, current three-dimensional reconstruction has many deficiencies：First, it is poor to soft tissue three-dimensional reconstruction ability.Skin
Middle collagen fiber, elastic fiber are difficult to be imaged；Secondly, CT, MRI resolution is relatively low.Such as computed tomography (CT) space point
Resolution is for about 400 microns, therefore, it is difficult to identification heterogeneous microstructure；Laser scanning co-focusing microscope (CLSM) though have compared with high score
Resolution (less than 1 micron), but there is fluorescent labeling.
Skin is the important natural cover for defense for protecting body, and the organ that human body is maximum.Organization engineering skin is body surface
One of skin injury (burn, shaping, beauty) optimal substitute.
Organization engineering skin support can be divided into containing cytoskeleton and Acellular matrix according to cell divide is whether there is at present；According to
Support hierarchical classification is divided into single-layer bracket and double-layer scaffold；Natural material support and synthetic can be divided into according to raw material classification
Stock support.Natural material support is referred to (plant or animal origin) protein-based, polysaccharide etc. present in nature
The support that natural material builds.Wherein protein includes collagen, fibronectin etc., and polysaccharide includes (alginate, viscous many
Sugar, shitosan, hyaluronic acid, chondroitin sulfate, gelatin etc..Such as chitosan-gelatin-hyaluronic acid (Cs-Gel-HA) porous
Frame, shitosan/collagen scaffold of glutaraldehyde cross-linking etc..Synthetic material support mainly includes hydroxy acid derivative, lactic acid
Derivant and other polyester fiber derivants.At present report have lactic-co-glycolic acid-collagen network polymerss, collagen-
The laminated film of polycaprolactone, polyvidone (Povidone)-polysaccharide etc..
The artificial skin Integra (Life Science) of the first commercialization is that a kind of collagen class corium is substituted in the world
Product.Its lower floor is formed with 6- chondroitin sulfate (GAG) composite crosslinking by the I-type collagen of cattle, in vesicular texture, hole
Footpath 30-120um；It is made up of silicon rubber film on upper strata.Another kind of artificial skin Apligraf (Organogenesis) is by cattle flesh
The collagen cross-linking of tendon collagen extraction, includes the fibroblast from neonatal foreskin separation and Culture, while on surface grafting
Chrotoplast.
However, current tissue engineering product still has many deficiencies, particularly bionical degree is not high, and (dermal scaffold fiber is
Random distribution, different from human physiological structure), cause effect unsatisfactory.
In April, 2013, in the 6th national organizational project and regenerative medicine conference (Xi'an), Zeng You expert proposes, material
Scholar is difficult to manufacture highly bionical biological support, is because that anatomist can not provide detailed structured data.It is regrettable
, in application on human skin, there is not been reported for the graphics of the spatial distribution of majority fibers (collagen fiber, elastic fiber).
The content of the invention
The technical problem to be solved in the present invention is for majority fibers composition mesh structure three-dimensional visible model in application on human skin
Set up and a kind of new selection is provided.
Majority fibers composition mesh structure three-dimensional visible model in application on human skin is the technical scheme is that, using such as lower section
Method is prepared：
A, tissue slice：Fresh cadaver femoribus internus full thickness skin is taken, fixative is fixed, paraffin embedding, cut into slices；
B, dyeing：Aldehyde-fuchsin staining dyeing is carried out to the tissue slice obtained by step a；
C, image acquisition：Cut into slices using panoramic scanning microscope scanning step b, obtained section panorama sketch；
D, image registration：Choose the continuous and picture comprising same hair follicle from the panoramic pictures of step c, intercept per
The hair follicle region in picture is ROI (Region of Interest need region to be processed in picture), with the hair follicle is
Registration marks with the hair follicle as registration marks, using 18.01 software of Adobe Photoshop CS5 softwares or PhotoLine
Carry out manual registration；
E, image binaryzation process：Image segmentation is carried out to the image after registration in step d, collagen fiber, bullet is partitioned into
The region of property fiber, using Matlab 7.10.0 softwares, Microsoft Visual Studio 2008, Microsoft
GCC softwares under Visual C++6.0, dev-c++5.5.3 or Linux system, obtain collagen fiber, elastic fiber respectively
Binary map；
F, three-dimensional reconstruction visualization：Collagen fiber that step e is obtained, the binary map of elastic fiber is directed respectively into three-dimensional can
Depending on changing software；Thus the three-dimensional digital model of collagen fiber, elastic fiber can be derived respectively, or/and is obtained comprising above two
The three-dimensional digital model of fiber.
Wherein, cut into slices 200 in step a, slice thickness is 5～8 μm.Section less than 5 μm is easily damaged, it is difficult to make
It is standby；Section more than 8 μm then can large losses fault information.
Specifically, in step a, fixative is Zamboni's fixatives, and its collocation method is paraformaldehyde 20g, and saturation is bitter
Sour in the mouth 150mL, plus Karasson-Schwlt ' s PB (Karasson-Schwlt ' s phosphate buffers) to 1000mL；Wherein
Karasson-Schwlt ' s PB collocation methods are to take NaH2PO4·H2O 3.31g, Na2HPO4·7H2O 33.77g, plus double steamings
Water is to 1000mL.
Specifically, in step b reagent needed for aldehyde-fuchsin staining include aldehyde-fuchsin dye liquor, 0.5% potassium permanganate solution,
1% oxalic acid aqueous solution, 50% ethanol, 70% ethanol, 95% ethanol, 100% ethanol, 1% viride nitens solution, xylene solution；Its
In, aldehyde-fuchsin dyeing recipe is：The ethanol 100mL of basic fuchsin 0.5g, concentrated hydrochloric acid 1mL, paraldehydum 1mL, 70%.
Wherein, picture is complete is chosen in step d, it is without breakage, non-wrinkled；Stock-dye is clear, distinct with surrounding contrast
Panoramic pictures.
Wherein, in step f, three-dimensional visualization software is 10.01 softwares of Materialise Mimics, and Thresholds sets
It is set to Min:226,Max:3056.
Present invention also offers majority fibers composition mesh structure three-dimensional visible model is in 3D printing in described application on human skin
In purposes.
Present invention also offers majority fibers composition mesh structure three-dimensional visible model is in bionical material in described application on human skin
Purposes in material preparation.
Preferably, described biomimetic material is prepared as the design of skin biomimetic scaffolds.
Present invention also offers majority fibers composition mesh structure three-dimensional visible model is given birth in skin in described application on human skin
Purposes in reason functional study.
Specifically, described skin physiology functional study is skin fiber morphological assessment, fiber content calculating or skin power
Learn specificity analysises.
The inventive method can the network structure of majority fibers composition in skin (collagen fiber, elastic fiber) is clear,
Intuitively, high-resolution to show, skin microstructure is clear, high resolution, and resolution is less than 1 micron.Resulting mould
Type is highly bionical, and fiber distribution is identical with normal human.The threedimensional model can be used for fiber shape in normal skin and scar tissue
State assessment, cubage and other physiological function researchs.In addition to the skin, the program can be used for other soft tissues, the three of organ
Dimension is rebuild, and makes up conventional image technology for the deficiency of soft tissue reconstruction.The model can be used as high biomimetic biomaterial
Template.The threedimensional model is expected to be applied to high bionical industry, manufacture and the most close bionics skin of organization of human body.The model can be with
The control system of 3D printer is introduced directly into, instructs 3D printer to carry out printing.
Description of the drawings
Fig. 1：Fresh cadaver femoribus internus full thickness skin (is fixed in Zamboni's fixatives)
Fig. 2：Skin biopsy panorama sketch (skin corium thickness about 1.46mm, collagen fiber green, and elastic fiber purple,
The excellent PRECICS500 that receives in Beijing)
Fig. 3 a：Some dermis region with hair follicle (in figure, lower left corner hair follicle is registration marks)
Fig. 3 b：After image segmentation, collagen fiber binary map
Fig. 3 c：After image segmentation, elastic fiber binary map
Fig. 4：(yellow represents collagen fiber model to application on human skin fibre composition network structure three-dimensional visible model, and green is represented
Elastic fiber model) Fig. 4 a:Side view Fig. 4 b:Front elevation
Fig. 5：The netted structure three-dimensional visual model Fig. 5 a of application on human skin collagen fiber:Side view Fig. 5 b:Front elevation
Fig. 6：The netted structure three-dimensional visual model Fig. 6 a of application on human skin elastic fiber:Side view Fig. 6 b:Front elevation
Fig. 7：3D printer control system operation interface, the interface in left figure printing, right figure are the interface after the completion of printing
Fig. 8：The collagen fiber illustraton of model 8a that 3D printer is printed:Side view Fig. 8 b:Front elevation
Fig. 9：Collagen fiber scattergram in each layer of corium.From shallow to deep, collagen fiber first increase corium, reduce afterwards.
Figure 10：Elastic fiber scattergram in each layer of corium.From shallow to deep, elastic fiber first increases corium, reduces afterwards.
Figure 11：Sectioning image after dyeing
Figure 12：It is three parts by Figure 11 image division.A:Hair follicle area B：Near follicles C：Away from hair follicle area
Figure 13：Acellular dermal (ADM) internal orifice dimension scattergram
Figure 14：Acellular dermal (ADM) inner pore wall thickness distribution figure
Figure 15:MicroCT scans people's acellular dermal (ADM) image scale：1mm
Figure 15 A：Front elevation Figure 15 B：One of cross section Figure 15 C：The two of cross section
Figure 15 D：Back view Figure 15 E：One of longitudinal section Figure 15 F：The two of longitudinal section
Figure 15 G：Graph of pore diameter distribution (different colours represent different pore size size)
Figure 15 H：One of graph of pore diameter distribution longitudinal section
Figure 15 I：The two of graph of pore diameter distribution longitudinal section
Specific embodiment
Image registration accuracy directly influences the verity of three-dimensional reconstruction.Image registration choose 149 sequence numbers it is continuous and
Picture comprising same hair follicle.Hair follicle region in intercepting per pictures.With the hair follicle as registration marks, using Adobe
Photoshop CS5 softwares carry out manual registration.Image after registration save as BMP forms picture (size 3140*3004,
24).For disappearance or the picture for damaging, then replicate upper pictures to substitute.Can also using other have image rotation,
Other image processing softwares of translation, transparence, full-filling function and figure layer process function, such as 18.01 softwares of PhotoLine.
Specifically, step a section number is relevant with object module thickness by slice thickness.200 are cut into slices such as, thickness is 5 μ
M, then object module thickness is 1000 μm.
Wherein, the image in step d after registration saves as the picture of BMP forms, size 3140 × 3004,24.Specifically
Picture format and size may be selected.Its determiner is：Later stage disposal ability；The width of object module；Need to include complete
Histological structure.
Wherein, choosing the picture standard that is from the Zhang Quanjing picture of step c is：Picture is complete, it is without breakage, non-wrinkled；
Stock-dye is clear, distinct with surrounding contrast.
Wherein, picture size is converted to into 1024 × 979 after image segmentation in step e.Specific picture format and size
May be selected.Its determiner is：Later stage disposal ability；The width of object module；Complete histological structure need to be included.
Fresh cadaver used in the present invention refers to the corpse in dead 24 hours, and skin is not dehydrated, and does not have necrosis.
Embodiment 1 obtains majority fibers composition mesh structure three-dimensional visible model in application on human skin using the inventive method
1) skin histology microscopic section：Take fresh cadaver femoribus internus full thickness skin (1cm × 1cm × 1cm, the 3rd medical officer
University southwest hospital burn department skin storehouse provides), skin edge is pruned, and (see Fig. 1) is fixed using Zamboni's fixatives.By group
Knitting block carries out being dehydrated, after transparent, paraffin embedding, continuously cuts section 200 using histotome (Leica).Per a section
5 microns of spacing.Section is laid in into anticreep slide (Hui Li Reagent Companies) central authorities, by the sequencing label for cutting.
Zamboni's fixes formula of liquid：Paraformaldehyde 20g, saturation picric acid 150mL, plus Karasson-Schwlt ' s
PB to 1000ml.Wherein Karasson-Schwlt ' s PB formula：NaH2PO4H2O 3.31g, Na2HPO47H2O 33.77g,
Plus distilled water is to 1000mL.
2) collagen fiber, elastic fiber dyeing：To step 1) in carry out aldehyde-fuchsin staining dyeing per section, collagen is fine
Green is tieed up, elastic fiber purple.
Reagent needed for aldehyde-fuchsin staining：Aldehyde-fuchsin dye liquor, 0.5% potassium permanganate solution, 1% oxalic acid aqueous solution, ethanol
(concentration is respectively 50%, 70%, 95%, 100%), 1% viride nitens solution, xylene solution.Wherein aldehyde-fuchsin dyeing recipe is：
The ethanol 100mL of basic fuchsin 0.5g, concentrated hydrochloric acid 1ml, paraldehydum 1mL, 70%.
3) panoramic picture of cutting into slices is gathered：It is (excellent to receive scientific and technological UNIC Technologies Inc. using panoramic scanning microscope
Match 500 serial 40 times of Armed Pathology Deparments of Full automatic digital pathology scanning system object lens of farsighted PRECICS) scanning step is 2)
In per section, obtain 200 section panorama sketch, see Fig. 2.
4) image registration：From step 3) 200 section panorama sketch in, choose 149 sequence numbers continuous and comprising same
The picture of hair follicle.The hair follicle region in intercepting per pictures is ROI.ROI：W=4000, H=4000, Area：1140624(μ
m×μm).With the hair follicle as registering (Fig. 3 a), manual registration is carried out using Adobe Photoshop CS5 softwares.After registration
Image saves as the picture (size 3140 × 3004,24) of BMP forms.For disappearance or the picture for damaging, then upper one is replicated
Pictures are substituting.
5) image binaryzation is processed：To step 4) in registration after image carry out image segmentation.Purpose is to be partitioned into collagen
The region of fiber, elastic fiber.Picture size is converted to into 1024 × 979.Using Matlab 7.10.0 (R2010a,
MathWorks) software, obtains collagen fiber, the binary map (see Fig. 3 b and 3c) of elastic fiber respectively.
6) three-dimensional reconstruction visualization：By step 5) collagen fiber that obtain, the binary map of elastic fiber be directed respectively into
10.01 softwares of Materialise Mimics.Thresholds is set to Min:226,Max:3056.Thus can derive respectively
The three-dimensional digital model of collagen fiber, elastic fiber, it is also possible to obtain the three-dimensional digital model comprising above two fiber.
Threedimensional model sectional drawing is Fig. 4,5,6.It can be seen that collagen fiber are thicker, compact structure；Elastic fiber is thinner, and structure is dredged
Pine, it is identical with normal human.
2 image registration of embodiment
1) Adobe Photoshop CS5 softwares are opened, imports No. 1 picture (reference base picture).
2) No. 2 pictures are imported, setting opacity is 50%-70%.Open free mapping function (Ctrl+T).With No. 1
Hair follicle in picture is registration marks, freely converts No. 2 slice positions, reaches two pictures and at utmost overlap.Recover No. 2
Picture opacity is 100%.No. 2 pictures are saved as into BMP forms (Ctrl+S).
3) No. 3 pictures are imported, is operated according to the method described above successively.
According to aforesaid operations, image registration can be completed.
3 3D printer of embodiment prints the feasibility test of the threedimensional model
By taking collagen fiber three-dimensional visible model as an example：
1st, the collagen fiber three-dimensional visible model that derivation has been rebuild from 10.01 softwares of Materialise Mimics
(stl forms).
2nd, by model importing 3D printer control software (in southwest hospital of Third Military Medical University joint surgery organizational project
Heart Zprint450 three-dimensional printers) 3D printer control system operation interface is shown in Fig. 7.
3rd, using gypsum powder be raw material, start printing.Printing is finished.Take 52 points 47 seconds, using 16.3mL glue
Water.The model for printing is about 5cm × 5cm × 1cm.
Printing is finished, as shown in Figure 8.Indentation, there is hair follicles locations, it is seen that a large amount of pore structures.Although printing raw material is stone
Cream powder, rather than collagen fiber, but the operation confirms that it is feasible to print the threedimensional model using 3D printer.
In 4 application on human skin skin corium of embodiment, collagen fiber, elastic fiber analysis of distribution from shallow to deep
1st, intercept skin corium region picture (ROI) in panorama sketch.The drawing software carried using windows 2007, filling
The non-fiber such as hair follicle, stain, sweat gland composition is white.
2nd, using Matlab 7.10.0 (R2010a, MathWorks) software, skin corium is equally divided into into 10 layers, calculates every
In one layer, the ratio of the total pixel of collagen fiber pixel, elastic fiber pixel and this layer the results are shown in Table 1.
3rd, with method 2, skin corium is equally divided into into 100 layers, is calculated in each layer, collagen fiber pixel, elastic fiber picture
Element and the ratio of this layer of total pixel, are as a result shown in Fig. 9 and Figure 10.
4th, Mathematical Statistics Analysis are carried out to data using 13.0 softwares of SPSS.
1 corium delamination of table is analyzed
| The number of plies | Distance (mm) away from epidermis dermis boundary | Collagen fiber account for the ratio of this layer | Elastic fiber accounts for the stratum proportion |
| 1 | 0.146 | 0.1444 | 0.0504 |
| 2 | 0.292 | 0.1950 | 0.0680 |
| 3 | 0.438 | 0.2075 | 0.0650 |
| 4 | 0.584 | 0.2137 | 0.0689 |
| 5 | 0.730 | 0.2103 | 0.0680 |
| 6 | 0.876 | 0.2005 | 0.0685 |
| 7 | 1.022 | 0.1860 | 0.0661 |
| 8 | 1.168 | 0.1855 | 0.0660 |
| 9 | 1.314 | 0.1711 | 0.0603 |
| 10 | 1.460 | 0.1317 | 0.0517 |
13.0 statistic analysis results of SPSS：
(1) collagen fiber are distributed with significant difference in high dermis and deep layer.(F=2.861, P=0.003)
1st layer is considerably less than 2-6 layers, and the 10th layer is considerably less than 2-8 layers, and between 2-9 layer corium, there was no significant difference.
(2) elastic fiber is distributed with significant difference in high dermis and deep layer.(F=4.089, P=0.000)
1st layer, the 10th layer is considerably less than 2-9 layers；There was no significant difference for the distribution of 2-9 layer intradermal elastic fiber.
In sum, corium bottommost layer, the distribution of most shallow-layer fiber are minimum, corium intermediate distribution equilibrium zero difference.
In 5 application on human skin skin corium of embodiment near follicles with away from hair follicle area collagen fiber, the elastic fiber regularity of distribution point
Analysis
1st, Adobe Photoshop CS5 softwares, skin corium region picture (ROI) being directed respectively in embodiment 4 are opened.
Using selection and the filling function of software, filling hair follicle (A) is redness, and near follicles (B) are green.Away from hair follicle area (C) no
Filling.Figure 11 is seen before filling, sees Figure 12 after filling.
2nd, the ratio of following groups using Matlab 7.10.0 (R2010a, MathWorks) software, is calculated respectively：
First group：1. (collagen fiber+elastic fiber)/B 2. (collagen fiber+elastic fiber)/C
Second group：3. collagen fiber/B 4. collagen fiber/C
3rd group：5. elastic fiber/B 6. elastic fiber/C
3rd, Mathematical Statistics Analysis are carried out to data using 13.0 softwares of SPSS.
The results are shown in Table 2.
2 application on human skin near follicles of table, the difference for going fiber distribution away from hair follicle
Note：In upper table, B is near follicles, and C is away from hair follicle area.
Embodiment 6MicroCT scanning people's acellular dermal (ADM)
The present embodiment can obtain a large amount of anatomical datas, such as porosity, pore diameter, hole wall thickness etc..Because people
Acellular dermal (ADM) is to be prepared to get by application on human skin, so the data are also applied for model of the present invention, can be to model of the present invention
Carry out quantization supplement.
1st, de- people's cell corium (ADM, Beijing name of the last ruler of the Xia Dynasty Ya Laifu Bioisystech Co., Ltd, J-1 types, allogeneic) is taken, is repaiied
Cutting edge edge.
2nd, (Ioversol, Chinese medicines quasi-word H20041796, Jiangsu perseverance are auspicious above-mentioned people's acellular dermal to be soaked into ioversol
Medical limited company), about 10 minutes.
3rd, people's acellular dermal is taken out, wipes dry unnecessary ioversol liquid, be put into MicroCT (SCANCO Medical AG
VivaCT40, great Ping hospitals of Third Military Medical University aseptic sursery institute) it is scanned.
Key parameter is set to：Voxelsize(μm):10.5；Energy/Intensity:45KVp,88μA,4W；
Number of Slices:213；Time:11.6min(46mAs).Gauss Sigma:.8；Gauss Support:1；Lower
Threshold:680,Upper Threshold:1000.The end of scan, analytical data.As a result Figure 13～15 are seen.
As a result it is as follows：
1st, pixel shared by porosity=hole/(pixel shared by pixel+pore wall shared by hole)
Porosity is：75.7%
2nd, pore diameter (the pore size)
Average diameter：0.1207mm medians：0.0945mm
3rd, hole wall thickness (the wall thickness)
Average thickness：0.1138mm medians：0.0525mm
Claims (9)
1. in application on human skin majority fibers composition mesh structure three-dimensional visible model preparation method, it is characterised in that：Using as follows
Method is prepared：
A, tissue slice：Fresh cadaver femoribus internus full thickness skin is taken, fixative is fixed, paraffin embedding, cut into slices；The fixative
For Zamboni's fixatives, its collocation method is paraformaldehyde 20g, saturation picric acid 150mL, plus Karasson-Schwlt's
Phosphate buffer is to 1000mL；Wherein Karasson-Schwlt's PB collocation methods are to take NaH2PO4·H2O 3.31g,
Na2HPO4·7H2O 33.77g, plus distilled water is to 1000mL；
B, dyeing：Aldehyde-fuchsin staining dyeing is carried out to the tissue slice obtained by step a；Reagent needed for aldehyde-fuchsin staining
Including aldehyde-fuchsin dye liquor, 0.5% potassium permanganate solution, 1% oxalic acid aqueous solution, 50% ethanol, 70% ethanol, 95% ethanol,
100% ethanol, 1% viride nitens solution, xylene solution；Wherein, aldehyde-fuchsin formula for dye liquor is：Basic fuchsin 0.5g, concentrated hydrochloric acid
The ethanol 100mL of 1mL, paraldehydum 1mL, 70%；
C, image acquisition：Cut into slices using panoramic scanning microscope scanning step b, obtained section panorama sketch；
D, image registration：The continuous and picture comprising same hair follicle is chosen from the panoramic pictures of step c, is intercepted per pictures
In the hair follicle region；With the hair follicle as registration marks, using Adobe Photoshop CS5 softwares or PhotoLine18.01
Software carries out manual registration；
E, image binaryzation process：Image segmentation is carried out to the image after registration in step d, collagen fiber, elasticity is partitioned into fine
The region of dimension, using Matlab 7.10.0 softwares, Microsoft Visual Studio 2008, Microsoft Visual
GCC softwares under C++6.0 or dev-c++5.5.3, Linux system, obtain collagen fiber, the binary map of elastic fiber respectively；
F, three-dimensional reconstruction visualization：Collagen fiber that step e is obtained, the binary map of elastic fiber are directed respectively into three-dimensional visualization
Software Materialise Mimics 10.01 or Amira 5.4.3 softwares；Thus collagen fiber, elastic fiber can be derived respectively
Three-dimensional digital model, or/and obtain the three-dimensional digital model comprising collagen fiber and elastic fiber.
2. in application on human skin as claimed in claim 1 majority fibers composition mesh structure three-dimensional visible model preparation method, its
It is characterised by：In step a, slice thickness is 5～8 μm.
3. in application on human skin as claimed in claim 1 or 2 majority fibers composition mesh structure three-dimensional visible model preparation method,
It is characterized in that：Choose picture is complete in step d, without damaged, non-wrinkled, stock-dye is clear, and distinct complete of surrounding contrast
Scape picture.
4. in application on human skin as claimed in claim 3 majority fibers composition mesh structure three-dimensional visible model preparation method, its
It is characterised by：In step f, three-dimensional visualization software is 10.01 softwares of Materialise Mimics, and Thresholds is set to
Min:226,Max:3056。
5. with majority fibers composition mesh structure three-dimensional visible in the application on human skin of the method preparation described in any one of claim 1-4
Purposes of the model in 3D printing.
6. with majority fibers composition mesh structure three-dimensional visible in the application on human skin of the method preparation described in any one of claim 1-4
Purposes of the model in biomimetic material preparation.
7. purposes as claimed in claim 6, it is characterised in that：Described biomimetic material is prepared as setting for skin biomimetic scaffolds
Meter.
8. can with majority fibers composition mesh structure three-dimensional in the application on human skin of the method preparation described in any one of Claims 1 to 4
Purposes of the perceived model in skin physiology functional study.
9. purposes as claimed in claim 8, it is characterised in that：Described skin physiology functional study is commented for skin fiber form
Estimate, fiber content is calculated or skin mechanics specificity analysises.
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| US6633408B1 (en) * | 1999-06-29 | 2003-10-14 | Kodak Polychrome Graphics, Llc | Spectral modeling of photographic printing based on dye concentration |
| CN102930602A (en) * | 2012-10-20 | 2013-02-13 | 西北大学 | Tomography-image-based facial skin three-dimensional surface model reconstructing method |
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| 人体尺神经显微结构三维可视化研究;刘艇 等;《中国修复重建外科杂志》;20080930;第22卷(第9期);1026-1030 * |
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