CN103760211A - Minor tissue hyalinizing micro liquid flow electrophoresis tank plate - Google Patents
Minor tissue hyalinizing micro liquid flow electrophoresis tank plate Download PDFInfo
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- CN103760211A CN103760211A CN201410037724.3A CN201410037724A CN103760211A CN 103760211 A CN103760211 A CN 103760211A CN 201410037724 A CN201410037724 A CN 201410037724A CN 103760211 A CN103760211 A CN 103760211A
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Abstract
The invention relates to the technical field of experimental instruments and discloses a minor tissue hyalinizing micro liquid flow electrophoresis tank plate. The electrophoresis tank plate comprises a fixing plate and at least two electrophoresis tanks uniformly arranged and fixed on the fixing plate, wherein electrophoresis liquid tanks are arranged at the front parts of the electrophoresis tanks; electrode tanks are arranged at the rear parts of the electrophoresis tanks; seal baffles are arranged between the electrophoresis liquid tanks and the electrophoresis tanks; a liquid inlet and a liquid outlet are formed in the left side and the right side of each electrophoresis liquid tank respectively; positive and negative electrodes are arranged in each electrode tank; a platinum electrode wire is connected to the bottom of each of the positive and negative electrodes; the platinum electrode wires penetrate through the seal baffles to extend into the electrophoresis liquid tanks. The electrophoresis tank plate is simple in structure and convenient to use, can be used for flexibly performing electrophoresis treatment, micro liquid flow treatment or the electrophoresis treatment and the micro liquid flow treatment on one or more samples to hyalinize tissues for the convenience of subsequent dyeing and observation steps, and meets personalized and batch experiment and production requirements.
Description
Technical field
The present invention relates to test apparatus technical field, relate in particular to a kind of microtissue transparence microstream electrophoresis frid.
Background technology
Electrophoresis is that charged particle is under electric field action, towards moving with its electrically contrary electrode.Electrophoresis is applied to the every field such as analytical chemistry, biological chemistry, clinical chemistry, toxic agent, pharmacology, immunology, microbiology, Food Chemistry increasingly extensively.The required instrument of electrophoresis has: electrophoresis tank and power supply.Existing electrophoresis tank is larger, and is generally used for molecular biology electrophoresis experiment, is unfavorable for the processing of microtissue, and existing electrophoresis tank can not carry out batch processing, to laboratory, great amount of samples is processed and has been brought inconvenience.
Summary of the invention
The present invention aims to provide a kind of microtissue transparence microstream electrophoresis frid, not only well address the above problem, and simple in structure, easy to use, when sample is carried out to microstream rinsing, carry out electrophoretic process, also facilitate great amount of samples is carried out to batch processing simultaneously.
Technical scheme of the present invention is a kind of microtissue transparence microstream electrophoresis frid, comprise fixed head, electrophoresis tank, electrophoresis tank has at least two, electrophoresis tank is uniformly arranged and fixed on fixed head, electrophoresis tank front portion is electrophoresis liquid bath, electrophoresis tank rear portion is slot electrode, between electrophoresis liquid bath and slot electrode, be provided with seal diaphragm, the electrophoresis liquid bath left and right sides is respectively arranged with inlet opening, fluid hole, in slot electrode, be provided with positive and negative electrode, positive and negative electrode bottom is all connected with platinum electrode silk, and platinum electrode silk extends in electrophoresis liquid bath through seal diaphragm.
Preferably, described positive and negative electrode is copper electrode.
Further, described inlet opening is higher than fluid hole.
Preferably, described electrophoresis tank has 6,12,24,48,96, and described electrophoresis tank is matrix and arranges on fixed head.
Further, with the fluid hole of a line electrophoresis liquid groove, be connected successively by pipeline with the inlet opening of adjacent electrophoresis liquid bath, the inlet opening of first electrophoresis liquid bath of every row is connected with input duct, and the fluid hole of last electrophoresis liquid bath of every row is connected with fluid pipeline.
Preferably, on described input duct and fluid pipeline, be provided with valve, described input duct is connected with peristaltic pump.
Further, described slot electrode top is provided with slot electrode lid, and slot electrode covers and is provided with two electrode sockets, and described two electrode sockets are connected with positive and negative electrode respectively.
Further, also comprise the attaching plug suitable with electrode socket, described attaching plug top is provided with insulator cap.
The invention has the beneficial effects as follows: simple in structure, easy to use, can to single or a plurality of samples, carry out electrophoretic process, microstream is processed or carry out electrophoretic process and microstream processing simultaneously flexibly, make transparency of organization, be convenient to follow-up dyeing and observe step, satisfying personalized and mass experiment and Production requirement.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the perspective view that in Fig. 1, electrophoresis tank 2 amplifies;
Fig. 3 is the vertical view of Fig. 2;
Fig. 4 is the sectional view of A-A direction in Fig. 3;
Fig. 5 is the perspective view of electrode 13 in Fig. 4;
In figure: 1. fixed head, 2. electrophoresis tank, 3. input duct, 4. pipeline, 5. fluid pipeline, 6. electrophoresis liquid bath, 7. slot electrode, 8. slot electrode lid, 9. electrode socket, 10. fluid hole, 11. inlet openings, 12. platinum electrode silks, 13. electrodes, 14. seal diaphragms.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing, the present invention is further elaborated.
As shown in Fig. 1 to 5, the invention provides a kind of microtissue transparence microstream electrophoresis frid, comprise fixed head 1, electrophoresis tank 2, electrophoresis tank 2 has at least two, can to a plurality of samples, carry out electrophoretic process so simultaneously, meet laboratory and great amount of samples has been carried out the requirement of batch processing under identical conditions.Electrophoresis tank 2 is uniformly arranged and fixed on fixed head 1, electrophoresis tank 2 front portions are electrophoresis liquid bath 6, electrophoresis tank 2 rear portions are slot electrode 7,7 of electrophoresis liquid bath 6 and slot electrodes are provided with seal diaphragm 14, electrophoresis liquid bath 6 left and right sides are respectively arranged with inlet opening 11, fluid hole 10, in slot electrode 7, be provided with positive and negative electrode 13, positive and negative electrode 13 bottoms are all connected with platinum electrode silk 12, and platinum electrode silk 12 extends in electrophoresis liquid bath 6 through seal diaphragm 14.Positive and negative electrode 13 is copper electrode.The height of inlet opening 11 is higher than fluid hole 10, and like this during the liquid flow in electrophoresis liquid bath 6, liquid oblique flow, has increased the orientation of liquid flow, has prevented that liquid from only flowing at a surface level, improved the effect of microstream rinsing greatly.Electrophoresis tank 2 has 6,12,24,48,96, and electrophoresis tank 2 is matrix and arranges on fixed head 1.Fluid hole 10 with a line electrophoresis liquid groove 6 is connected with the inlet opening 11 of adjacent electrophoresis liquid bath 6 by pipeline 4 successively, the inlet opening 11 of first electrophoresis liquid bath 6 of every row is connected with input duct 3, and the fluid hole 10 of last electrophoresis liquid bath 6 of every row is connected with fluid pipeline 5.On input duct 3 and fluid pipeline 5, be provided with valve, input duct 3 is connected with peristaltic pump.In use, input duct 3 is connected with the container that elution buffer is housed, fluid pipeline 5 and elution buffer coated lycopene, the peristaltic pump in input duct 3 provides power for elution buffer.Elution buffer is slowly flowed to fluid pipeline 5 from input duct 3, when like this sample being carried out to microstream rinsing, carry out electrophoresis, can make lipid material generation electric field migration time of organization internal by elution buffer wash-out, thereby it is inner to make albumen, nucleic acid, the polysaccharide molecule of organization internal be retained in pretreated hydrogel nanometer framework.In this process, operator can, by the valve on switch input duct 3 and fluid pipeline 5, control elution buffer interior the flowing of electrophoresis liquid bath 6 of needs.
Fixed head 1 can be in the same size with existing Tissue Culture Plate, the length of electrophoresis tank 2 and width also can with existing Tissue Culture Plate on cell culture hole diameter consistent.When the electrophoresis tank 2 on fixed head 1 is 6, the length and width of fixed head 1 are high is 126.6 * 85.4 * 20.1mm, and electrophoresis liquid bath 6 length are 33～35mm, and wide is 16～17mm, and applicable sample size is 33 * 16 * 2mm; When the electrophoresis tank 2 on fixed head 1 is 12, the length and width of fixed head 1 are high is 126.6 * 85.4 * 20.1mm, and electrophoresis liquid bath 6 length are 22～24mm, and wide is 11～12mm, and applicable sample size is 22 * 11 * 2mm; When the electrophoresis tank 2 on fixed head 1 is 24, the length and width of fixed head 1 are high is 126.6 * 85.4 * 20.1mm, and electrophoresis liquid bath 6 length are 15～16mm, and wide is 7～8mm, and applicable sample size is 15 * 7 * 2mm; When the electrophoresis tank 2 on fixed head 1 is 48, the length and width of fixed head 1 are high is 126.6 * 85.4 * 20.1mm, and electrophoresis liquid bath 6 length are 10～11mm, and wide is 5～6mm, and applicable sample size is 10 * 5 * 2mm; When the electrophoresis tank 2 on fixed head 1 is 96, the length and width of fixed head 1 are high is 126.6 * 85.7 * 14.3mm, the long 6～7mm of electrophoresis liquid bath 6, and wide is 3～4mm, applicable sample size is 6 * 3 * 1.4mm.Be that fixed head 1 length is 120～130mm, wide is 80～90mm; Electrophoresis liquid bath 6 length are 5～40mm, and wide is 3～20mm.Be convenient to like this test consumptive material unitized unitized with production mould, reduce costs, also met the demand that small sample is processed, while not needing to process small sample again, also adopt large equipment, reduced energy consumption and improved treatment effect.Certainly the electrophoresis tank 2 on fixed head 1 can be arbitrary arrangement, is preferably matrix and arranges.
In use, operator can need to select different operations according to different.When experiment sample only needs to carry out electrophoretic process, operator can switch on power the electrophoresis tank of needs 2 to carry out electrophoresis, closes the valve of input duct 3 and fluid pipeline 5, or seals inlet opening 11 and fluid hole 10 on each electrophoresis liquid bath 6.When experiment sample only needs to carry out microstream rinsing, operator can not be communicated with power supply, only input duct 3 is connected with the container that elution buffer is housed, fluid pipeline 5 and elution buffer coated lycopene, open peristaltic pump in input duct 3 and provide power for elution buffer, elution buffer is flowed, carry out microstream wash-out.When both having needed to carry out electrophoretic process, experiment sample had need to carry out microstream rinsing and process time, operator can carry out two parts operation above simultaneously, the electrophoresis tank of needs 2 is switched on power and carries out electrophoresis, input duct 3 is connected with the container that elution buffer is housed simultaneously, fluid pipeline 5 and elution buffer coated lycopene, open peristaltic pump in input duct 3 for elution buffer provides power, elution buffer is flowed, reach the object of carrying out microstream rinsing processing.When can carry out microstream rinsing to sample like this, carry out electrophoresis, again can be according to different sample sizes carry out single sample process or a plurality of sample batch processing, satisfying personalized and mass experiment and Production requirement.
Certainly; the present invention also can have other various embodiments; in the situation that not deviating from spirit of the present invention and essence thereof; those of ordinary skill in the art can make according to the present invention various corresponding changes and distortion, but these corresponding changes and distortion all should belong to the protection domain of the appended claim of the present invention.
Claims (9)
1. a microtissue transparence microstream electrophoresis frid, it is characterized in that: comprise fixed head, electrophoresis tank, described electrophoresis tank has at least two, electrophoresis tank is uniformly arranged and fixed on fixed head, described electrophoresis tank front portion is electrophoresis liquid bath, electrophoresis tank rear portion is slot electrode, between electrophoresis liquid bath and slot electrode, be provided with seal diaphragm, the described electrophoresis liquid bath left and right sides is respectively arranged with inlet opening, fluid hole, in described slot electrode, be just provided with, negative electrode, just described, negative electrode bottom is all connected with platinum electrode silk, described platinum electrode silk extends in electrophoresis liquid bath through seal diaphragm.
2. microtissue transparence microstream electrophoresis frid according to claim 1, is characterized in that: described positive and negative electrode is copper electrode.
3. microtissue transparence microstream electrophoresis frid according to claim 1, is characterized in that: described inlet opening is higher than fluid hole.
4. microtissue transparence microstream electrophoresis frid according to claim 1, is characterized in that: described fixed head length is 120～130mm, and wide is 80～90mm, and described electrophoresis liquid flute length is 5～40mm, and wide is 3～20mm.
5. microtissue transparence microstream electrophoresis frid according to claim 1, is characterized in that: described electrophoresis tank has 6,12,24,48,96, and described electrophoresis tank is matrix and arranges on fixed head.
6. microtissue transparence microstream electrophoresis frid according to claim 5, it is characterized in that: the fluid hole with a line electrophoresis liquid groove is connected with the inlet opening of adjacent electrophoresis liquid bath by pipeline successively, the inlet opening of first electrophoresis liquid bath of every row is connected with input duct, and the fluid hole of last electrophoresis liquid bath of every row is connected with fluid pipeline.
7. microtissue transparence microstream electrophoresis frid according to claim 6, is characterized in that: on described input duct and fluid pipeline, be provided with valve, described input duct is connected with peristaltic pump.
8. microtissue transparence microstream electrophoresis frid according to claim 1, is characterized in that: described slot electrode top is provided with slot electrode lid, and slot electrode covers and is provided with two electrode sockets, and described two electrode sockets are connected with positive and negative electrode respectively.
9. microtissue transparence microstream electrophoresis frid according to claim 8, is characterized in that: also comprise the attaching plug suitable with electrode socket, described attaching plug top is provided with insulator cap.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410037724.3A CN103760211B (en) | 2014-01-26 | 2014-01-26 | A kind of microtissue transparence microstream electrophoresis frid |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410037724.3A CN103760211B (en) | 2014-01-26 | 2014-01-26 | A kind of microtissue transparence microstream electrophoresis frid |
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| CN103760211A true CN103760211A (en) | 2014-04-30 |
| CN103760211B CN103760211B (en) | 2016-03-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356653A (en) * | 2017-08-21 | 2017-11-17 | 莫纳（苏州）生物科技有限公司 | Integral type electrophoresis apparatus |
| CN108627563A (en) * | 2017-03-17 | 2018-10-09 | 王志伟 | A kind of electrophoretic apparatus and electrophoresis system and transparence tissue mark method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1828295A (en) * | 2006-04-06 | 2006-09-06 | 上海交通大学 | Device for researching isoelectrofocusing electrophoresis dynamics |
| CN201669062U (en) * | 2010-05-05 | 2010-12-15 | 乐山师范学院 | Electrophoresis tank for recovering electrophoresed gel DNA |
| CN103331099A (en) * | 2013-06-28 | 2013-10-02 | 上海交通大学 | Automatic heat dissipation type free-flow electrophoresis separation chamber device |
| CN203688497U (en) * | 2014-01-26 | 2014-07-02 | 成都医学院 | Micro liquid flow electrophoresis tank plate for enabling micro tissue to be transparent |
-
2014
- 2014-01-26 CN CN201410037724.3A patent/CN103760211B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1828295A (en) * | 2006-04-06 | 2006-09-06 | 上海交通大学 | Device for researching isoelectrofocusing electrophoresis dynamics |
| CN201669062U (en) * | 2010-05-05 | 2010-12-15 | 乐山师范学院 | Electrophoresis tank for recovering electrophoresed gel DNA |
| CN103331099A (en) * | 2013-06-28 | 2013-10-02 | 上海交通大学 | Automatic heat dissipation type free-flow electrophoresis separation chamber device |
| CN203688497U (en) * | 2014-01-26 | 2014-07-02 | 成都医学院 | Micro liquid flow electrophoresis tank plate for enabling micro tissue to be transparent |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108627563A (en) * | 2017-03-17 | 2018-10-09 | 王志伟 | A kind of electrophoretic apparatus and electrophoresis system and transparence tissue mark method |
| CN107356653A (en) * | 2017-08-21 | 2017-11-17 | 莫纳（苏州）生物科技有限公司 | Integral type electrophoresis apparatus |
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| Publication number | Publication date |
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| CN103760211B (en) | 2016-03-16 |
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