CN103756986B - A kind of method of comprehensive utilization of rice germination product - Google Patents
A kind of method of comprehensive utilization of rice germination product Download PDFInfo
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- CN103756986B CN103756986B CN201310399335.0A CN201310399335A CN103756986B CN 103756986 B CN103756986 B CN 103756986B CN 201310399335 A CN201310399335 A CN 201310399335A CN 103756986 B CN103756986 B CN 103756986B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/20—Malt products
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
Abstract
The invention provides a kind of method of comprehensive utilization of rice germination product.Select the paddy with germinating power or stay embryo brown rice or stay embryo coated mill rice to germinate after the long 20mm ~ 60mm of bud, collect the plumule, fibrous root and the de-bud root rice that are separated and produce, dried de-bud root rice can be used as feed, edible sprout brown rice, germinated milled rice or powder material.The levigate rear degreasing of dry bud root chopping, can obtain rice bud root grease and degreasing bud root.In 5%(w/w) ~ 15%(w/w) ratio trehalose is dissolved in 10%(w/w) ~ 20%(w/w) and glutaraldehyde solution in, then in 0.5:1(w/w) ~ 1:1(w/w) ratio this mixed solution is joined in degreasing bud root, degreasing bud root immobilized lipase is obtained after mixing, drying, this enzyme can repeatedly for fat hydrolysis catalyzed reaction, after reusing 6 times, its vigor still keeps more than 70%.
Description
Technical field
The invention belongs to a kind of invention about rice germination product; More particularly, the present invention relates to the method for comprehensive utilization that one utilizes lipase, grease and the degreasing product produced in rice (comprise paddy, stay embryo brown rice and stay embryo coated mill rice) germination product.
Background technology
Lipase, as the focus product of green chemical industry, is mainly obtained by microorganisms producing at present
[1].
On the other hand in rice containing abundant lipase, but because of its to the rice bran that rice storage ageing, brown rice pearling produce become sour destruction and always repel by people and discard.Rice lipase is mainly present in meter Pi and rice embryo, rice hulling obtains brown rice, namely the cortex sloughed in brown rice pearling processing and plumule are rice brans, account for 7% of brown rice gross weight, rice bran receives much concern because being rich in grease (accounting for the 16%-22% of rice bran gross weight) and abundant nutritive ingredient and physiologically active substance.But rice bran very easily becomes sour, its lipase when rice storage because structure is not destroyed, directly do not contact with fat, and make the slow Deterioration of paddy, but under rice bran grinds, when its cellularstructure is destroyed, lipase then contacts with grease and by prompt activation, namely rice bran was become sour in several hours rotten, the deep development seriously hindering rice bran utilizes.Therefore, for a long time, enzyme is concentrated on to the research of rice tikitiki lipase both at home and abroad and live in the extraction of various functional component after stabilization research and the enzyme that goes out and applied research, seldom utilize the research of rice lipase
[2-3].1997, FadnavisNW etc.
[4]the research in past is concentrated on and knocks out rice bran fats enzymic activity instead of utilize its high reactivity to feel to puzzle very in surprise, deliver the result of study that tikitiki lipase is applied to enantioselective hydrolysis reaction, shown the mankind and the one of rice lipase changing waste into valuable is expected.2009, Li Lili etc.
[5]have studied preparation and the biochemical property thereof of defatted rice bran fixed fat enzyme, have made some progress.But fail practical because its existence takies the shortcoming of rice bran resource.Up to now, more have no utilize rice germination give birth to the report of plumule and fibrous root (being called for short bud root) lipase.
Reference:
[1] Tong Zhiyong. green syt new page [J] is opened in lipase production domesticization. modern chemical industry, 2009,29(5): 91.
[2] Lv Yingguo, Ji Hui, Zhang Hui, etc. the comprehensive utilization [M] of rice bran resource. grain and feed industries, 2009(4): 19 ~ 22.
[3] Zhou Jianmin, Hu Zhanqiang, Tang Xiaozhi. the stabilization of rice bran and functional ingredient progress [J]. processing of farm products, 2012,8(12): 89 ~ 93,137.
[4]FADNAVISNW,JADHAVV.Enantioselectivehydrolyticreactionsofricebranlipase(RBL):afirstreport[J].Tetrahedron:Asymmetry,1997,8(14):2361~2366.
[5] Li Lili, Wu Hui, Wu Suxi, etc. the preparation of Rice Bran Solid immobilized lipase and biochemical Quality Research [J]. modern food science and technology, 2009,25 (7): 760 ~ 763,785.
Summary of the invention
Main purpose of the present invention and meaning: be to provide a kind of method of comprehensive utilization to rice (comprise paddy, stay embryo brown rice or stay embryo coated mill rice) germination processed products, mainly utilize rice germination gained bud root lipase and feature without the need to taking rice bran resource, rice bud root lipase is used, available bud root oil and degreasing bud root immobilized lipase can be increased on the basis retaining original Sprout rice and the rice bran utilization of resources, thus can significantly improve the added value of Rice producing, there is good economic benefit and social benefit.
The present invention realizes above-mentioned purpose by following steps:
(1) germinate and be separated: selecting the rice germination 4d ~ 10d with germinating power or after arriving the long 20mm ~ 60mm of bud, the long 30mm ~ 80mm of root, collect and the plumule of generation, fibrous root artificial or mechanical means separated from germination rice, residuum is de-bud root rice;
(2) dry and pulverizing: by separating obtained de-bud root rice, plumule, fibrous root at room temperature or 25 DEG C ~ 35 DEG C dry 24h ~ 60h of loft drier; Again dried plumule, fibrous root are chopped up mixing, be then finely ground to the material of 20 order ~ 80 mesh sieves with ultrafine crusher;
(3) degreasing: be 1:3(w/w by pulverizing and sieving the ratio of material with extraction agent) ~ 1:30(w/w) the above-mentioned material that pulverizes and sieves is added extraction agent carry out degreasing at 35 DEG C ~ 55 DEG C temperature, namely obtain the degreasing bud root of rice bud root grease and lipase activity survival, wherein extraction agent is recyclable reuses;
(4) intensive treatment: first add water and prepare 10%(w/w) ~ 20%(w/w) glutaraldehyde solution, again in 5%(w/w) ~ 15%(w/w) ratio add trehalose and be dissolved in wherein, then in 0.5:1(w/w) ~ 1:1(w/w) ratio this mixed solution is joined in degreasing bud root, 25 DEG C ~ 45 DEG C, 300rpm ~ 500rpm stirring 30min ~ 180min mixing, active protection is carried out to lipase and strengthens fixing process;
(5) dry: said mixture is dry, obtain degreasing bud root immobilized lipase.
Rice described in step (1) be have germinating power paddy or stay embryo brown rice or stay embryo coated mill rice.
Bud after step (2) described drying and crushing, root are used for extracting bud root oil and being used as immobilized lipase.And the further intermittently drying 5min ~ 30min of microwave that the de-bud root rice of step (2) described drying can be aided with 500W ~ 900W is to moisture 12% ~ 15%, as feed, edible sprout brown rice, edible germinated milled rice or edible rice flour raw material.
Degreasing described in step (3) can be 1:3 (w/w) ~ 1:30(w/w by pulverizing and sieving the ratio of material with extraction agent) add boiling point in the sherwood oil of 30 DEG C ~ 60 DEG C or ether or acetone degreasing under normal pressure (0.1MPa) and 30 DEG C ~ 55 DEG C temperature; Also can be that above-mentioned material is loaded degreasing at 30MPa ~ 35MPa and 30 DEG C ~ 40 DEG C temperature in container by CO 2 supercritical equipment requirements.Namely obtain the degreasing bud root of rice bud root grease and lipase activity survival, wherein extraction agent is recyclable reuses.
Trehalose described in step (4) and glutaraldehyde are food grade or the analytical pure commodity of the purity more than 80% that market is bought.
Step (5) described drying is atmospheric pressure at room or 25 DEG C ~ 55 DEG C dry 24h ~ 60h of loft drier.
The present invention adds available bud root oil and degreasing bud root immobilized lipase on the basis retaining original germination rice and the rice bran utilization of resources, significantly improve the added value of Rice producing, its principal feature is, on the basis not affecting former rice and rice bran main application (as staying its rice bran of embryo coated mill rice to grind its use), add bud root oil and the degreasing bud root immobilized lipase of rich activity and nutritive value.As de-bud root paddy can make feed, de-bud root brown rice and de-bud root coated mill rice can do edible sprout brown rice, germinated milled rice or edible rice flour raw material, and the new bud root obtained can extract edible or industrial bud root grease, degreasing bud root immobilized lipase eliminates the Isolation and purification technique of lipase, repeated multiple timesly can be used as fat hydrolysis catalysts especially after intensive treatment, there is wide utility value.
Its leading indicator has: every 1000g rice can obtain 800g ~ 1000g bud root; bud root oil 10g ~ 30g can be obtained; its lipase activity of bud root of germination 4d ~ 7d reaches the highest; for 6.25U/g; through the degreasing bud root immobilized lipase of Trehalosc protection and glutaraldehyde cross-linking, catalyzing oil hydrolysis reaction can be recycled and reused for, as reaction repeated 6 times; its enzymic activity still keeps more than 70%, significantly improves than the activity of the de-bud root immobilized lipase not carrying out trehalose and glutaraldehyde combination intensive treatment.
Specific embodiment:
Specific embodiment 1
(1) germinate and be separated: selecting the paddy 1000g with germinating power and germinate under 29 DEG C ~ 32 DEG C room temperatures after immersion 1.5h, 1 maintenance of every 8 hours water sprays is moistening, is separated the plumule of generation, the de-bud root paddy of bud root that fibrous root obtains 898.34g weight in wet base and 1055.09g weight in wet base after 7d;
(2) dry and pulverize: will separating obtained de-bud root paddy and bud root at 35 DEG C of loft drier drying 24h, obtain the bud root of 450.17g drying, be finely ground to 30 mesh sieves with ultrafine crusher again after being chopped up and obtain 365.14g material.Dried de-bud root paddy 738.52g obtains 664.65g paddy with the further intermittently drying 20min of the microwave of 700W, can do feedstuff raw material;
(3) degreasing: the above-mentioned material 365.84g that pulverizes and sieves is added 1500mL sherwood oil carry out degreasing at 40 DEG C of temperature, the degreasing bud root immobilized lipase that 26.58g bud root grease and lipase activity close 5.32U/g can be obtained, get wherein 300g be divided into 3 parts for subsequent use.The recyclable recycling of sherwood oil;
(4) the fixing process of glutaraldehyde strengthening: get and prepare 15%(w/w in advance) glutaraldehyde solution 50mL, then joined in 1 part of degreasing bud root (100g) of step (3) gained, 30 DEG C, 300rpm stirring 30min mixing, active protection is carried out to lipase and is cross-linked fixing process;
(5) trehalose and glutaraldehyde combined treatment: first prepare in advance containing 15%(w/w) glutaraldehyde and 10%(w/w) the solution 100mL of trehalose, join in 1 part of degreasing bud root (100g) of step (3) gained, 30 DEG C, 300rpm stirring 30min mixing, active protection is carried out to lipase and strengthens fixing process;
(6) dry: by the degreasing bud root of step (3) ~ (5) gained 3 kinds of different treatment at room temperature or 40 DEG C of dry 24h of loft drier, to obtain degreasing bud root immobilized lipase, can be used for hydrolysate oil and catalytic esterification.
Specific embodiment 2
The 3 kinds of immobilized lipases of gained in specific embodiment 1 are carried out fat hydrolysis reaction control experiment, respectively get 10g sample, add 10ml sweet oil is substrate at every turn, with its ppl event degree of Copoloid colorimetric method for determining (with reference to Xu Bin. the rapid assay methods of lipase activity in grain and processing byproduct thereof: China, 201110043917.6), 2 times are got express developed with 95% ethanol after every secondary response, then reuse, in the enzyme activity of first hydrolysis reaction for 100%, after repeating 6 uses, its enzyme activity still maintains the activity (as shown in table 1) of 70%
The relation of number of times and relative reactivity reused by table 1
From table 1, the de-bud root fixed fat enzymic activity that the ratio with trehalose and glutaraldehyde combined treatment does not carry out combined treatment significantly improves.
Specific embodiment 3
(1) germinate and be separated: selecting the embryo brown rice 1000g that stays with germinating power and germinate under 29 DEG C ~ 32 DEG C room temperatures after immersion 1h, 1 maintenance of every 8 hours water sprays is moistening, is separated the plumule of generation, the de-bud root brown rice of bud root that fibrous root obtains 801.05g weight in wet base and 664.65g weight in wet base after 7d;
(2) dry and pulverize: will separating obtained de-bud root brown rice and bud root at room temperature or 35 DEG C of loft drier drying 24h, the bud root of 409.04g drying can be obtained, be finely ground to 30 mesh sieves with ultrafine crusher again after being chopped up and obtain 318.75g material.Dried de-bud root brown rice 497.30g obtains 447.58g brown rice with the further intermittently drying 20min of the microwave of 700W, can do edible sprout brown rice, edible rice flour raw material or feed;
(3) degreasing: the above-mentioned material 318.75g that pulverizes and sieves is carried out carbon dioxide upercritical fluid extraction degreasing at high pressure 30MPa, 38 DEG C of temperature; The degreasing bud root immobilized lipase of 22.83g bud root grease and lipase activity conjunction 6.25U/g can be obtained.The recyclable recycling of sherwood oil;
(4) trehalose and glutaraldehyde combined treatment: first prepare in advance containing 15%(w/w) glutaraldehyde and 10%(w/w) the solution 300mL of trehalose, join in step (3) gained degreasing bud root, 30 DEG C, 300rpm stirring 30min mixing, active protection is carried out to lipase and strengthens fixing process;
(5) dry: by (4) gained mixture at room temperature or 40 DEG C of dry 24h of loft drier, to obtain degreasing bud root immobilized lipase, can be used for hydrolysate oil and catalytic esterification.
Specific embodiment 4
(1) germinate and be separated: selecting the embryo coated mill rice 1000g sterilized water that stays with germinating power and clean, then join with the hypochlorite solution of 0.2mol/L and flood rice and be advisable, carry out surface sterilization 60min, drain to be placed in thermostat container and germinate at 28 DEG C of temperature, take out every 8h and spray sterilized water once, after keeping moistening germination 7d, plumule, the fibrous root of collecting separation generation can obtain the bud root of 750.39g weight in wet base and the de-bud root coated mill rice of 651.75g weight in wet base;
(2) dry and pulverize: will separating obtained de-bud root coated mill rice and bud root at room temperature or 35 DEG C of dry 24h of loft drier, bud root coated mill rice must be taken off can do edible germinated milled rice, edible rice flour or feedstuff raw material by 423.64g after drying.Obtain the bud root of 389.40g drying, after being chopped up, obtain 319.92g material with ultrafine crusher levigate mistake 30 mesh sieve again;
(3) degreasing: the above-mentioned material 319.92g that pulverizes and sieves is added 1500mL sherwood oil carry out degreasing at 40 DEG C of temperature, can obtain the degreasing bud root immobilized lipase that 19.72g bud root grease and lipase activity close 4.71U/g.The recyclable recycling of sherwood oil;
(4) trehalose and glutaraldehyde combined treatment: first prepare in advance containing 15%(w/w) glutaraldehyde and 10%(w/w) the solution 300mL of trehalose, join in the degreasing bud root of step (3) gained, 30 DEG C, 300rpm stirring 30min mixing, active protection is carried out to lipase and strengthens fixing process;
(5) dry: by the degreasing bud root of step (3) ~ (5) gained 3 kinds of different treatment at room temperature or 40 DEG C of dry 24h of loft drier, to obtain degreasing bud root immobilized lipase, can be used for hydrolysate oil and catalytic esterification.
Claims (5)
1. a method of comprehensive utilization for rice germination product, the plumule produced in rice germination process, fibrous root are carried out being separated processing with de-bud root rice, it is characterized in that, comprise the following steps:
(1) germinate and be separated: selecting the rice germination 4d ~ 10d with germinating power or after arriving the long 20mm ~ 60mm of bud, the long 30mm ~ 80mm of root, collect and the plumule of generation, fibrous root artificial or mechanical means separated from germination rice, residuum is de-bud root rice;
(2) dry and pulverizing: by separating obtained de-bud root rice, plumule, fibrous root at the dry 24h ~ 60h of 25 DEG C ~ 35 DEG C loft drier, dried plumule, fibrous root chop up mixing, are then finely ground to the material of 20 order ~ 80 mesh sieves with ultrafine crusher;
(3) degreasing: be 1:3(w/w by pulverizing and sieving the ratio of material with extraction agent) ~ 1:30(w/w) the above-mentioned material that pulverizes and sieves is added extraction agent carry out degreasing at 30 DEG C ~ 50 DEG C temperature, namely obtain the degreasing bud root of rice bud root grease and lipase activity survival, wherein extraction agent is recyclable reuses;
(4) intensive treatment: first add water and prepare 10%(w/w) ~ 20%(w/w) glutaraldehyde solution, again in 5%(w/w) ~ 15%(w/w) ratio add trehalose and be dissolved in wherein, then in 0.5:1(w/w) ~ 1:1(w/w) ratio this mixed solution is joined in degreasing bud root, 25 DEG C ~ 45 DEG C, 300rpm ~ 500rpm stir 30min ~ 180min mixing;
(5) dry: said mixture is dry, obtain degreasing bud root immobilized lipase.
2. method according to claim 1, is characterized in that, the rice described in step (1) be have germinating power paddy, stay embryo brown rice or stay embryo coated mill rice.
3. method according to claim 1, it is characterized in that, the degreasing described in step (3) is 1:3(w/w by pulverizing and sieving the ratio of material with extraction agent) ~ 1:30(w/w) add boiling point 30 DEG C ~ 60 DEG C sherwood oil, ether or acetone degreasing under normal pressure and 30 DEG C ~ 50 DEG C temperature.
4. method according to claim 1, is characterized in that, the trehalose described in step (4) and glutaraldehyde are food grade or the analytical pure commodity of the purity more than 80% that market is bought.
5. method according to claim 1, is characterized in that, step (5) described drying is normal pressure and 25 DEG C ~ 35 DEG C dry 24h ~ 60h of loft drier.
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2013
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| KR20030080512A (en) * | 2002-04-09 | 2003-10-17 | 주식회사다스 | Revolution device of seat for vehicle |
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