CN103751257A - Amino acid oral liquid and preparation method thereof - Google Patents
Amino acid oral liquid and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an amino acid oral liquid and a preparation method thereof. The amino acid oral liquid comprises, by weight, 20-50% of silkworm chrysalis amino acid powder and 50-80% of soybean amino acid powder. The preparation method comprises the following steps of dissolution, mixing, filtration, bottling and disinfection. The amino acid oral liquid is safe and nontoxic, is convenient for eating, contains abundant proteins and a plurality of amino acids, greatly improves immunity and does not cause drug dependence after use for a long time.
Description
Technical field
The invention belongs to oral liquid field, relate to a kind of oral liquid of enhancing immunity, especially a kind of amino acids oral-liquor and preparation method thereof.
Background technology
Abundant amino acids from silkworm pupa contains rich in protein, fat, unsaturated fatty acid, a small amount of lecithin, vitamin with plurality of inorganic salt, its protein content can reach 68% and contain 17 seed amino acids, amino acid content is high, contribute to supplement the aminoacid that human body lacks, really played and maintained human body nitrogen balance, improved human body metabolic function, enhancing human body immunity systemic-function, the effect of enhancing human body immunity systemic-function, has the effect of enhancing human body immunity power.
Canaline is by soybean protein system, and there is abundant amino acid and protein in its China and Kazakhstan, and soybean protein is not homogeneous albumen, mainly 11S globulin and 7S, consists of, and its nutritive value is not second to high-quality animal proteinum.
Although two seed amino acids have good effect to human body, take and inconvenience.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art part, a kind of amino acids oral-liquor and preparation method thereof is provided, amino acids oral-liquor prepared by the present invention contains canaline and abundant amino acids from silkworm pupa, it contains rich in protein and several amino acids, has obvious enhancing immunity effect, simultaneously it has that preparation method is simple, effect outstanding feature.
The technical scheme that the present invention realizes object is as follows:
Amino acids oral-liquor, the percentage by weight of composition is as follows:
Canaline powder: 20%-50%, abundant amino acids from silkworm pupa powder: 50%-80%.
And described canaline powder, abundant amino acids from silkworm pupa powder meet the regulation in 2010 editions two of < < Chinese Pharmacopoeia > >.
The preparation method of amino acids oral-liquor, the step of method is as follows:
(1) by canaline powder with quality multiple 3-7 doubly, 50-80 ℃ of hot water dissolves, and obtains feed liquid A;
(2) by abundant amino acids from silkworm pupa powder with quality multiple 3-7 doubly, 20-40 ℃ of hot water dissolves, and obtains feed liquid B;
(3) by feed liquid A, B mixes, and stirs 30 minutes, filters, and obtains filtrate 1;
Advantage of the present invention and beneficial effect are:
Amino acids oral-liquor prepared by the present invention contains canaline and abundant amino acids from silkworm pupa, and it contains rich in protein and several amino acids, has obvious enhancing immunity effect, simultaneously it has that preparation method is simple, effect outstanding feature.
The specific embodiment
Below in conjunction with embodiment, amino acids oral-liquor of the present invention and preparation method thereof is further illustrated, following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Embodiment 1
Amino acids oral-liquor, composition following (percentage by weight):
Canaline powder: 80%, abundant amino acids from silkworm pupa powder: 20%
The preparation method of amino acids oral-liquor is as follows:
(1) by 3 times of quality multiples for canaline powder, 50 ℃ of hot water dissolve, and obtain feed liquid A;
(2) by 7 times of quality multiples for abundant amino acids from silkworm pupa powder, 40 ℃ of hot water dissolve, and obtain feed liquid B;
(3) by feed liquid A, B mixes, and stirs 30 minutes, filters, and obtains filtrate 1;
In the present embodiment, from dissolving, mix, the operation that is filled into sterilizing is all carried out in 100,000 clean areas.
Embodiment 2
Amino acids oral-liquor, composition following (percentage by weight):
Canaline powder: 50%, abundant amino acids from silkworm pupa powder: 50%
The preparation method of amino acids oral-liquor is as follows:
(1) by 7 times of quality multiples for canaline powder, 80 ℃ of hot water dissolve, and obtain feed liquid A;
(2) by 3 times of quality multiples for abundant amino acids from silkworm pupa powder, 20 ℃ of hot water dissolve, and obtain feed liquid B;
(3) by feed liquid A, B mixes, and stirs 30 minutes, filters, and obtains filtrate 1;
In the present embodiment, from dissolving, mix, the operation that is filled into sterilizing is all carried out in 100,000 clean areas.
Embodiment 3
Amino acids oral-liquor, composition following (percentage by weight):
Canaline powder: 40%, abundant amino acids from silkworm pupa powder: 60%
The preparation method of amino acids oral-liquor is as follows:
(1) by 5 times of quality multiples for canaline powder, 70 ℃ of hot water dissolve, and obtain feed liquid A;
(2) by 5 times of quality multiples for abundant amino acids from silkworm pupa powder, 30 ℃ of hot water dissolve, and obtain feed liquid B;
(3) by feed liquid A, B mixes, and stirs 30 minutes, filters, and obtains filtrate 1;
filtrate 1 is carried out to fill, and sterilizing, obtains amino acids oral-liquor;
In the present embodiment, from dissolving, mix, the operation that is filled into sterilizing is all carried out in 100,000 clean areas.
The compound basis of composition of the present invention
Abundant amino acids from silkworm pupa contains rich in protein, aminoacid, fat, unsaturated fatty acid, a small amount of lecithin, vitamin and plurality of inorganic salt, its protein content can reach 68%, it contains several amino acids, can improve mouse cell immunity and the humoral immune function of immunologic hypofunction, increase the weight of immune organ spleen and thymus, can also strengthen the ability that mouse peripheral blood mononuclear phagocyte carbon is cleaned up, thereby there is good enhancing function of immune system, the effect of enhancing human body immunity power, canaline powder also contains rich in protein and aminoacid, its nutritive value is not second to high-quality animal proteinum, its amino acid content reaches more than 18 kinds, and contain essential amino acids in human body necessary 8.There is document to confirm, various essential amino acids in soybean protein are compared with balance, can meet the more than 2 years old demand of human body to essential amino acids completely, the effect that this has had for enhancing human body immunity power, the common prescription of abundant amino acids from silkworm pupa and canaline, also have rich in protein and several amino acids, strengthen function of immune system, and both compatibilities are without taboo.
The efficacy test of amino acids oral-liquor of the present invention, this zoopery adopt prepared by embodiment 1-3 amino acids oral-liquor.
Experiment material and method
1.1 samples: amino acids oral-liquor is brown liquid, and its human intaking amount is 20ml/60kg.BW
1.2 laboratory animals: 160 of 18-22g female SPF Kunming mouses.
1.3 experimental animal feeding conditions: SPFJi laboratory animal room.Temperature: 21.4-24.2 ℃, humidity: 50-62%.
1.4 dosage are selected: the human body recommended intake of amino acids oral-liquor is 20ml/60kg.BW, this experimental design basic, normal, high three dosage groups, be respectively 1.67ml/kg, 3.33ml/kg, 10.00ml/kg, be equivalent to 5,10,30 times of human body recommended intake.Measure respectively amino acids oral-liquor 8.4ml, 16.7ml, 50.0ml, all adding distil water, to 100ml, is mixed with solution, press 0.2ml/10g.BW gavage, matched group gives equivalent distilled water, once a day, give continuously 30 days, within after last administration 24 hours, measure indices.Laboratory animal is divided into 4 large group, and 40 every group, then every treated animal is divided into 4 groups, 10 every group.Wherein 1 group is carried out HC50 mensuration, antibody-producting cell detection, delayed allergy (the sufficient sole of the foot thickens method); 2 groups are carried out Turnover of Mouse Peritoneal Macrophages and engulf chicken red blood cell test, dirty/body ratio measurement; 3 groups are carried out carbon clearance butter; 4 groups are carried out mouse lymphocyte transformation experiment, the test of NK cytoactive detection.
1.5 instruments and reagent: clean bench, aseptic dissection equipment, RPMI1640 cell culture fluid, canavaline, isopropyl alcohol, MTT, slide gauge, SRBC, microsyringe, Dou Shi reagent, india ink, chicken red blood cell, LHD base fluid, timer, hemoglobin pipet, 722 type visible spectrophotometers, centrifuge, normal saline, Na2CO3,24 well culture plates, CO2 gas incubator, microplate reader.
1.6 experimental techniques:
1.6.1 internal organs/weight ratio pH-value determination pH: administration, after 30 days, takes spleen, thymus, calculates dirty/body ratio.
1.6.2 delayed allergy (the sufficient sole of the foot thickens method): administration 30 days, the 25th day lumbar injection 2%SRBC, immunity, after 4 days, is measured left back sole of the foot portion thickness, at measuring point injection 20%SRBC20 μ l, after 24 hours, again measures simultaneously.
1.6.3 the mouse spleen lymphocyte transformation experiment (mtt assay) that ConA induces: administration 30 days, the aseptic spleen of getting, makes single cell suspension, and Hank ' s liquid is washed 3 times, adjusts 3 * 106/ml of cell concentration.Divide two holes to add in 24 well culture plates cell suspension, every hole 1ml, a hole adds 75 μ lConA liquid (100 μ l/ml), and another hole, for contrast, is put in 5%CO2 incubator and is cultivated 72h.Cultivation finishes front 4h, and every hole sucks supernatant 0.7ml, adds 0.7ml not containing the RPMI1640 culture fluid of calf serum, adds MTT(5mg/ml simultaneously) 50 μ l/ holes, continue to cultivate 4h.After cultivation finishes, every hole adds 1ml acid isopropyl alcohol, and piping and druming evenly, dissolves purple crystal completely, with 570mm wavelength, measures optical density value.
1.6.4 the mensuration of half hemolysis value (HC50): administration 30 days, the 25th day lumbar injection 2%SRBC, immunity is after 5 days, pluck eyeball and collect serum, the serum 1ml that in vitro adds 300 times of dilutions, 10%SRBC0.5ml, complement 1ml, 37 ℃ of water-baths 20 minutes, ice bath cessation reaction, centrifugal, get supernatant 1ml, add Dou Shi reagent 3ml, 540mm colorimetric after 10 minutes.
1.6.5 antibody-producting cell detects: administration 30 days, and the 25th day lumbar injection 2%SRBC, immunity is after 5 days, dislocation is put to death, and gets spleen, and 200 eye mesh screens filter, with Hanks liquid, wash 3 times, each centrifugal 10min of 1000r/min, by cell suspension in 5mlRPMI640 culture fluid.By top layer culture medium heating for dissolving, 45 ℃ of water bath heat preservations, mix with 2 times of concentration Hanks liquid of equivalent, subpackage small test tube, every pipe 0.5ml, adds 50 μ l10%SRBC, 20 μ l splenocyte suspensions, mix, reviewing, cultivates in CO2 gas incubator one hour, adds the complement (1:10) with the dilution of SA buffer, continue to cultivate one hour counting hemolysis plaque number.
1.6.6 test clearly mice carbon corridor: administration is after 30 days, and the india ink of 3.5 times of dilutions of tail vein injection (every 10 grams of body weight 0.1ml), gets blood 20 μ l respectively at the 2nd, 10 minutes endocanthions, joins in 3ml1% sodium carbonate liquor 600nm colorimetric.Separately get liver, spleen is weighed, and calculates phagocytic index.
1.6.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method): administration is after 30 days, every Mus lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30min, animal is put to death in cervical vertebra dislocation, being faced upward position is fixed on Mus plate, abdominal skin is cut off in center, through Intraperitoneal injection normal saline 2ml, rotate Mus plate 1min, sucking-off abdominal cavity washing liquid 1ml, average mark drips on 2 microscope slides, put into the enamel bowl that is lined with wet gauze, in 37 ℃ of incubator incubation 30min, incubate complete, normal saline rinsing, dry, with 1:1 acetone methanol solution, fix, the 4%Giemsa 3min that dyes.Counting is engulfed the macrophage number of chicken red blood cell and the macrophage number of being engulfed.
1.6.8 NK cytoactive detection (determination of lactate dehydrogenase method): before experiment, 24h goes down to posterity YAC-1 cell (target cell) to cultivate with RPMI1640 complete culture solution and adjusts 4 * 105/ml of cell concentration.The aseptic spleen of getting, makes single cell suspension, and Hanks liquid is washed 3 times, and adjusting cell concentration is 2 * 107/ml.Get each 100 μ l(effect target of target cell and effector lymphocyte than 50:1), add U-shaped 96 well culture plates, target cell Spontaneous release hole adds target cell and each 100 μ l of culture fluid, the maximum release aperture of target cell adds each 100 μ l of nucleus 1%NP40, all establish three multiple holes, in 37 ℃, 5%CO2 incubator, cultivate 4h, draw at the bottom of supernatant 100 μ l horizontalizations in 96 well culture plates in every hole, add LDH base fluid 100 μ l simultaneously, reaction 3min, every hole adds the HCL30 μ l of 1mol/l, at microplate reader 492nm, measures optical density value.
1.7 results statistics: experimental data is carried out statistical test with SPSS11.5 for Windows, matched group is with experimental group employing variance analysis and check between two, and as heterogeneity of variance, person adopts data transaction, still unevenly after conversion adopts nonparametric statistics.
2 results
2.1 amino acids oral-liquors are on the impact of Mouse Weight (table 1-4)
Table 1 amino acids oral-liquor on the impact of immune 1 group of Mouse Weight (means standard deviation, g)
Dosage (g/kg) number of animals (only) the P value that increases weight latter stage mid-term at initial stage
Contrast 10 19.3 ± 0.8 27.7 ± 1.9 36.0 ± 2.8 16.6 ± 2.9-
1.67 10 19.4±0.9 27.4±2.6 35.9±1.9 16.5±2.1 0.914
3.33 10 19.2±0.9 28.0±2.9 35.7±2.8 16.5±2.8 0.914
10.00 10 19.2±0.9 28.1±1.7 35.5±2.2 16.3±2.8 0.803
Table 2 amino acids oral-liquor on the impact of immune 2 groups of Mouse Weights (means standard deviation, g)
Dosage (g/kg) number of animals (only) the P value that increases weight latter stage mid-term at initial stage
Contrast 10 19.7 ± 1.3 27.7 ± 2.5 35.8 ± 2.6 16.1 ± 2.3-
1.67 10 19.6±1.4 28.0±1.7 36.0±3.1 16.4±2.1 0.719
3.33 10 19.7±1.0 27.6±2.5 35.5±2.2 15.8±1.2 0.719
10.00 10 19.6±1.5 27.9±2.5 36.0±2.2 16.4±2.8 0.700
Table 3 amino acids oral-liquor on the impact of immune 3 groups of Mouse Weights (means standard deviation, g)
Dosage (g/kg) number of animals (only) the P value that increases weight latter stage mid-term at initial stage
Contrast 10 20.1 ± 1.3 29.2 ± 2.5 36.2 ± 2.8 16.1 ± 2.7-
1.67 10 20.2±1.3 29.5±2.3 36.4±1.8 16.2±2.0 0.926
3.33 10 20.2±1.3 29.4±2.5 36.6±2.1 16.4±2.4 0.774
10.00 10 20.1±1.1 29.4±2.0 36.0±2.9 16.5±3.3 0.755
Table 4 amino acids oral-liquor on the impact of immune 4 groups of Mouse Weights (means standard deviation, g)
Dosage (g/kg) number of animals (only) the P value that increases weight latter stage mid-term at initial stage
Contrast 10 19.3 ± 1.0 25.9 ± 2.1 32.7 ± 2.8 13.3 ± 1.2-
1.67 10 19.3±1.0 25.8±2.4 32.6±1.9 13.3±0.9 0.999
3.33 10 19.6±1.0 26.4±2.4 33.1±2.8 13.5±1.1 0.795
10.00 10 19.4±0.9 25.7±2.8 33.1±2.2 13.7±2.0 0.595
From table 1-4, per os gave the amino acids oral-liquor of various dose after 30 days, and each treated animal vegetative activity is good, each weightening finish of dosage treated animal and matched group comparison, and there are no significant for difference (P>0.05).
2.2 amino acids oral-liquors are on the impact of mice organs/body weight ratio (in Table 5)
Table 5 amino acids oral-liquor is on the impact of mice organs/body weight ratio (means standard deviation)
Group number of animals thymus/body weight ratio P value spleen/body weight ratio P value
Contrast 10 0.30 ± 0.07---0.44 ± 0.07---
1.67 10 0.30±0.07 0.541 0.44±0.07 0.905
3.33 10 0.30±0.07 0.731 0.43±0.09 0.872
From table 5, each dosage group mouse spleen, thymus/body weight ratio and matched group comparison, there are no significant for difference (P>0.05).
The impact of 2.3 amino acids oral-liquors on mouse cell immunologic function
2.3.1 amino acids oral-liquor is to mice delayed allergy (the sufficient sole of the foot thickens method) (in Table 6)
The impact (means standard deviation) of table 6 amino acids oral-liquor on delayed allergy (the sufficient sole of the foot thickens method)
Group number of animals is attacked front and back toes thickness difference P value
(ml/kg.bw) only) (mm)
Contrast 10 0.49 ± 0.08---
1.67 10 0.51±0.05 0.655
3.33 10 0.46±0.08 0.416
10.00 10 0.63±0.14* 0.003
From table 6, high dose group mice is attacked front and back toes thickness difference higher than matched group, and difference has significance (P<0.05).
2.3.2 the impact (in Table 7) of amino acids oral-liquor on the mouse spleen lymphocyte transformation experiment (mtt assay) of ConA induction
The impact of table 7 amino acids oral-liquor on the mouse spleen lymphocyte transformation experiment (mtt assay) of ConA induction
(means standard deviation)
Group number of animals optical density difference P value
(ml/kg.bw) (only)
Contrast 10 0.149 ± 0.038---
1.67 10 0.200±0.042* 0.024
3.33 10 0.156±0.043 0.753
10.00 10 0.194±0.064* 0.046
From table 7, the optical density difference of low, high dose group is all higher than matched group, and difference has significance (P<0.05).
The impact of 2.4 amino acids oral-liquors on humoral immunization
2.4.1 amino acids oral-liquor is on the impact of mice half hemolysis value (HC50) (in Table 8)
The impact of table eight amino acid Oral Liquid On Mice half hemolysis value (HC50)
(means standard deviation)
Group number of animals HC50 P value
Contrast 10 227.1 ± 22.8---
1.67 10 250.7±17.7* 0.021
3.33 10 262.8±27.3* 0.001
10.00 10 237.0±18.6 0.320
From table 8, the mice half hemolysis value (HC50) of low, middle dosage group is higher than matched group, and difference has significance (P<0.05).
2.4.2 the impact (in Table 9) of amino acids oral-liquor on the test of mouse antibodies cellulation
The impact of table nine amino acid Oral Liquid On Mice antibody-producting cell
(means standard deviation)
Group number of animals hemolysis plaque is counted P value
(ml/kg.bw) (only) (* 103/ full spleen)
Contrast 10 15.4 ± 4.4---
1.67 10 15.8±5.8 0.871
3.33 10 19.9±6.0 0.101
10.00 10 18.2±7.4 0.291
From table 9, the mouse antibodies cellulation of three dosage groups is compared with matched group, and there are no significant for difference (P>0.05).
The impact of 2.5 amino acids oral-liquors on monocytes/macrophages function
2.5.1 amino acids oral-liquor is on the impact of mice carbon clearance butter (in Table 10)
The impact of table ten amino acid Oral Liquid On Mice carbon clearance butter
(means standard deviation)
Group number of animals phagocytic index P value
Contrast 10 3.81 ± 0.60---
1.67 10 4.15±0.68 0.285
3.33 10 4.38±0.66 0.080
10.00 10 4.89±0.86* 0.001
From table 10, the phagocytic index of high dose group is higher than matched group, and difference has significance (P<0.05).
2.5.2 amino acids oral-liquor is engulfed the impact (in Table 11) of chicken red blood cell test on Turnover of Mouse Peritoneal Macrophages
Table 11 amino acids oral-liquor is engulfed the impact of chicken red blood cell test on Turnover of Mouse Peritoneal Macrophages
(means standard deviation)
Group number of animals phagocytic percentage P value phagocytic index P value
Contrast 10 25.0 ± 5.9---0.27 ± 0.07---
1.67 10 28.4±5.5 0.192 0.31±0.07 0.113
3.33 10 30.8±4.5 0.030 0.32±0.09 0.043
10.00 10 26.6±6.8 0.536 0.29±0.08 0.472
From table 11, the phagocytic percentage of middle dosage group and phagocytic index are higher than matched group, and difference has significance (P<0.05).
The impact (in Table 12) of 2.6 amino acids oral-liquor NK cytoactives
The impact (determination of lactate dehydrogenase method) of table 12 amino acids oral-liquor NK cytoactive
(means standard deviation)
Group number of animals NK cytoactive P value
Contrast 10 17.7 ± 4.6---
1.67 10 18.1±5.3 0.865
3.33 10 19.3±5.2 0.512
10.00 10 21.1±6.4 0.176
From table 12, the NK cytoactive of each dosage group is compared with matched group, and there are no significant for difference (P>0.05).
3 brief summaries
Per os gave the amino acids oral-liquor of various dose after 30 days, and each treated animal vegetative activity is normal.In delayed allergy (the sufficient sole of the foot thickens method), high dose group mice is attacked front and back toes thickness difference higher than matched group, and difference has significance (P<0.05); In the mouse spleen lymphocyte transformation experiment (mtt assay) of ConA induction, the optical density difference of low, high dose group is all higher than matched group, and difference has significance (P<0.05); During mice half hemolysis value (HC50) is measured, the mice half hemolysis value (HC50) of low, middle dosage group is higher than matched group, and difference has significance (P<0.05); In carbon clearance test, the phagocytic index of high dose group is higher than matched group, and difference has significance (P<0.05); Turnover of Mouse Peritoneal Macrophages is engulfed in chicken red blood cell test (half intracorporal method), and the phagocytic percentage of middle dosage group and phagocytic index are higher than matched group, and difference has significance (P<0.05).All other tests are showed no immunosuppressant phenomenon.Assay shows, amino acids oral-liquor has enhancing immunity function.
Claims (7)
1. amino acids oral-liquor, is characterized in that: the percentage by weight of composition is as follows:
Canaline powder: 20%-50%, abundant amino acids from silkworm pupa powder: 50%-80%.
2. amino acids oral-liquor according to claim 1, is characterized in that: the percentage by weight of composition is as follows:
Canaline powder: 40%, abundant amino acids from silkworm pupa powder: 60%.
3. according to the amino acids oral-liquor one of claim 1-2 Suo Shu, it is characterized in that: described canaline powder, abundant amino acids from silkworm pupa powder meet the regulation in 2010 editions two of < < Chinese Pharmacopoeia > >.
4. the preparation method of the amino acids oral-liquor as described in one of claim 1-3, is characterized in that: the step of method is as follows:
(1) by canaline powder with quality multiple 3-7 doubly, 50-80 ℃ of hot water dissolves, and obtains feed liquid A;
(2) by abundant amino acids from silkworm pupa powder with quality multiple 3-7 doubly, 20-40 ℃ of hot water dissolves, and obtains feed liquid B;
(3) by feed liquid A, B mixes, and stirs 30 minutes, filters, and obtains filtrate 1;
5. the preparation method of amino acids oral-liquor according to claim 4, is characterized in that: by 5 times of quality multiples for described canaline powder, 70 ℃ of hot water dissolve.
6. the preparation method of amino acids oral-liquor according to claim 4, is characterized in that: 5 times of quality multiples for described abundant amino acids from silkworm pupa powder, 30 ℃ of hot water dissolve.
7. the preparation method of amino acids oral-liquor according to claim 4, is characterized in that: described step
sterilising temp is 110 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539722A (en) * | 2016-11-25 | 2017-03-29 | 江苏爱西施科技服务咨询股份有限公司 | A kind of BB frosts wetting agent and preparation method thereof |
| CN106858614A (en) * | 2017-03-20 | 2017-06-20 | 安徽省华信生物药业股份有限公司 | Compound amino acid selenium yeast oral liquid |
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2013
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539722A (en) * | 2016-11-25 | 2017-03-29 | 江苏爱西施科技服务咨询股份有限公司 | A kind of BB frosts wetting agent and preparation method thereof |
| CN106858614A (en) * | 2017-03-20 | 2017-06-20 | 安徽省华信生物药业股份有限公司 | Compound amino acid selenium yeast oral liquid |
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Application publication date: 20140430 |