CN103750846A - Cobitidae fish blood fixation method - Google Patents
Cobitidae fish blood fixation method Download PDFInfo
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- CN103750846A CN103750846A CN201410036989.1A CN201410036989A CN103750846A CN 103750846 A CN103750846 A CN 103750846A CN 201410036989 A CN201410036989 A CN 201410036989A CN 103750846 A CN103750846 A CN 103750846A
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- blood
- cobitidae
- syringe
- cobitidae fish
- fish
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Abstract
The invention relates to a cobitidae fish blood fixation method. The method includes the steps that stationary liquid (sodium citrate or heparin) for coagulating blood of cobitidae fish is placed into an injector, the injector is utilized to directly collect blood samples from a cobitidae fish body and usually from the tail of the cobitidae fish body, blood collected from the cobitidae fish body is directly fixed after entering the injector due to fact that the stationary liquid (sodium citrate) is preplaced in the injector, and then the blood is sent to a laboratory for refrigeration and storage for usage in the following process. The blood samples are prevented from being wasted, the blood fixation process is simplified, and the method is easy to learn and suitable for coagulating blood of the cobitidae fish body.
Description
Technical field
The present invention relates to a kind of Cobitidae Fish Blood fixing means.
Background technology
Yarkant Triplophysa Triplophysa (Hedinichthys) yarkandensis (Day), local dog head fish by name, is subordinate to Cypriniformes, Cobitidae, Nemacheilinae, Genus Triplophysa, drum fish glue subgenus; Be distributed widely in Tarim River System, Tarimhe River Endemic fish, one of Fish that distribution height above sea level is the highest.In recent years, due to the pollution of Tarim River System water body, environmental disruption, Yarkant Triplophysa resource falls sharply, and may become the Fish in imminent danger of the third Tarimhe River after flat kiss fish and Tarim Basin schizothoracin, need to urgently protect.
In recent years, more for the research of Fish Physiology and biochemistry, especially, for the investigation of these easily endanger Fish or Fish living environments in imminent danger, the research of environment-stress etc., all must carry out hematological research.For individuality, compared with for large Fish, gather blood ratio and be easier to, but less for individuality, the Fish that blood is had relatively high expectations, are more difficult, add fixing, the thing being difficult to especially.
Summary of the invention
In view of Yarkant Triplophysa is alepidote class, individuality is less, and one of Fish that resource falls sharply, in order better to carry out the research of the aspects such as its Physiology and biochemistry, have carried out blood collection, hereby to carry out correlational study.
By the Yarkant Triplophysa gathering in advance in culturist's aquarium, blood to be collected.
To configure sodium citrate and be divided into more than 2 parts, in portion, place centrifuge tube blood sample to be added, in portion syringe to be put into.
Yarkant Triplophysa anaesthetized to Baoding or do not anaesthetized Baoding, with clean towel wiping fish body, especially caudal peduncle blood sampling place, keep clean, adopting the syringe of 1.0ml or 2.0ml, and in syringe, extract in advance a small amount of sodium citrate.
At Yarkant Triplophysa caudal peduncle tail vein blood, with extracting a small amount of sodium citrate blood collection needle, after entering syringe, blood is fixed immediately, in order to avoid extract overlong time, solidify.
The specific embodiment
Hereinafter, will describe more fully the present invention now, show various embodiment.Yet the present invention can implement in many different forms, and should not be interpreted as being confined to embodiment set forth herein.On the contrary, it will be thorough with completely providing these embodiment to make the disclosure, and scope of the present invention is conveyed to those skilled in the art fully.
The Yarkant Triplophysa of collection is cultivated in advance to blood to be collected in aquarium.
To configure sodium citrate and be divided into more than 2 parts, in portion, place centrifuge tube blood sample to be added, in portion syringe to be put into.
Yarkant Triplophysa anaesthetized to Baoding or do not anaesthetized Baoding, with clean towel wiping fish body, especially caudal peduncle blood sampling place, keep clean, adopting the syringe of 1.0ml or 2.0ml, and in syringe, extract in advance a small amount of sodium citrate.
At Yarkant Triplophysa caudal peduncle tail vein blood, with extracting a small amount of sodium citrate blood collection needle, after entering syringe, blood is fixed immediately, in order to avoid extract overlong time, solidify.
The foregoing is only embodiments of the invention, be not limited to the present invention.The present invention can have various suitable changes and variation.All any modifications of doing within the spirit and principles in the present invention, be equal to replacement, improvement etc., within protection scope of the present invention all should be included in.
Claims (4)
1. a Cobitidae Fish Blood fixing means, is characterized in that:
To first put into syringe for the fixing fixative of Cobitidae Fish Blood, then with the direct blood sample collection from Cobitidae Fish body of syringe, owing to there being the fixative of putting in advance in syringe, so be directly fixed after the blood gathering from Cobitidae Fish body enters syringe, deliver to again laboratory stored refrigerated, for future use.
2. a kind of Cobitidae Fish Blood fixing means as claimed in claim 1, is characterized in that:
Described fixative is sodium citrate or heparin.
3. a kind of Cobitidae blood fixing means as claimed in claim 1, is characterized in that:
Before described blood sample collection, Yarkant Triplophysa anaesthetized to Baoding or do not anaesthetized Baoding, with clean towel wiping fish body, especially caudal peduncle blood sampling place, keep clean, adopting the syringe of 1.0ml or 2.0ml, and in syringe, extract in advance a small amount of sodium citrate.
4. a kind of Cobitidae Fish Blood fixing means as claimed in claim 1, is characterized in that:
Described blood sample collection is at Yarkant Triplophysa caudal peduncle tail vein blood.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036989.1A CN103750846A (en) | 2014-01-24 | 2014-01-24 | Cobitidae fish blood fixation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410036989.1A CN103750846A (en) | 2014-01-24 | 2014-01-24 | Cobitidae fish blood fixation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103750846A true CN103750846A (en) | 2014-04-30 |
Family
ID=50518293
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410036989.1A Pending CN103750846A (en) | 2014-01-24 | 2014-01-24 | Cobitidae fish blood fixation method |
Country Status (1)
| Country | Link |
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| CN (1) | CN103750846A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167121A (en) * | 2020-10-16 | 2021-01-05 | 国家能源集团新疆开都河流域水电开发有限公司 | Method for cultivating long-body plateau loach fry |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998025523A1 (en) * | 1996-11-25 | 1998-06-18 | Symbiosis Corporation | Biopsy forceps instrument having irrigation and aspiration capabilities |
| JP2004290517A (en) * | 2003-03-27 | 2004-10-21 | Olympus Corp | Biopsy needle |
| CN101035459A (en) * | 2004-10-05 | 2007-09-12 | 奥林巴斯株式会社 | Endoscope system, biopsy-sample container, method of obtaining biopsy samples, and method of processing biopsy samples |
-
2014
- 2014-01-24 CN CN201410036989.1A patent/CN103750846A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998025523A1 (en) * | 1996-11-25 | 1998-06-18 | Symbiosis Corporation | Biopsy forceps instrument having irrigation and aspiration capabilities |
| JP2004290517A (en) * | 2003-03-27 | 2004-10-21 | Olympus Corp | Biopsy needle |
| CN101035459A (en) * | 2004-10-05 | 2007-09-12 | 奥林巴斯株式会社 | Endoscope system, biopsy-sample container, method of obtaining biopsy samples, and method of processing biopsy samples |
Non-Patent Citations (2)
| Title |
|---|
| 傅晓龙: "《大白鼠大剂量静脉血心脏快速采血方法的改进》", 《中国组织工程研究与临床康复》, vol. 13, no. 7, 12 February 2009 (2009-02-12), pages 1279 - 1282 * |
| 高建军: "《泥鳅的血液学及湖北省泥鳅群体的遗传多样性研究》", 《华中农业大学硕士学位论文》, 31 December 2010 (2010-12-31), pages 13 - 15 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112167121A (en) * | 2020-10-16 | 2021-01-05 | 国家能源集团新疆开都河流域水电开发有限公司 | Method for cultivating long-body plateau loach fry |
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Application publication date: 20140430 |