CN103739762A - Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere - Google Patents
Preparation method of near-ultraviolet polystyrene copolymerization fluorescence microsphere Download PDFInfo
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Abstract
The invention discloses a preparation method of a near-ultraviolet polystyrene copolymerization fluorescence microsphere, and the preparation method is as follows: putting initiator azodiisobutyronitrile and dispersing agent polyvinylpyrrolidone in absolute ethanol to obtain a clear and transparent solution, simultaneously adding styrene and N-allyl carbazole to the solution for copolymerization reaction under the protection of nitrogen to obtain a polystyrene copolymerization fluorescence microsphere, and then introducing sulfonic acid groups or hydroxyl groups onto the surface of the polystyrene copolymerization fluorescence microsphere to produce the functional near-ultraviolet polystyrene copolymerization fluorescence microsphere. The preparation method adopts a method of dispersion polymerization to prepare the near-ultraviolet polystyrene copolymerization fluorescence microsphere having good monodispersity and stable fluorescent properties.
Description
Technical field
The present invention relates to a kind of preparation method of fluorescent microsphere, particularly relate to the preparation method of the styrene copolymerized fluorescent microsphere of a kind of near ultraviolet functional polyalkylene.
Background technology
Fluorescent microsphere has stable morphological structure and efficient luminous efficiency, be subject to ambient conditions as less than pure fluorescent chemicals in the impact of solvent, heat, electricity, magnetic etc., especially biomedical sector has extremely important application in a lot of fields, as the detection of the detection of immunodetection, cell marking and high-flux medicaments sifting, immobilized enzyme, cell-surface antigens, degeneration DPN tracer, phagocytic function, gene studies, the quality control of susceptibility diagnostic reagent etc.Wherein the fluorescent microsphere of mono dispersed micrograde (1-10 μ m) application is very extensive, the particularly application in flow cytometer.
CN 1278534A discloses fluorescently-labeled polymer microsphere and preparation method thereof, first by Polymer Synthesizing method, makes nano level or micron-sized polymer microsphere, and prepares fluorescent microsphere with its absorption or bonding fluorescence molecule or fluorescent probe.In absorption fluorescent microsphere prepared by the method fluorescence dye easily leak, cancellation, in test, time can cause sample contamination.
Document (Dyes and Pigments, 2009,82,134-141) reported that take N-allyl group naphthalimide and vinylbenzene is raw material, adopt the method for emulsifier-free emulsion polymerization to prepare fluorescent microsphere, yet this fluorescent microsphere surface lacks functional group, the research of the aspects such as inconvenient follow-up biology.
As everyone knows, polystyrene microsphere has that size is controlled, good dispersity, specific surface area are large, have biologically inert, by common solvent, do not dissolved or swelling, adsorptivity is strong, coherency good, and the specific physique such as surface reaction activity height, the binding ability that materials such as protein, dyestuff, close ligand has been had, is well suited for the carrier as fluorescent substance in bioanalysis.Polystyrene microsphere is combined with the fluorescence dye with two keys, the morphological structure that it is stable and stable and efficient luminous efficiency, the application of organism being carried out to the aspects such as mark, detection and screening is a lot of.
But the fluorescence dye using in existing report is mostly to glow or the fluorescent material of green glow, does not use the fluorescent material of blue light-emitting.In addition, according to document (Journal of Materials Chemistry, 2009,19,2018-2025) report is larger owing to having the luminescent dye molecule of two keys, in polymerization process, there is to a certain degree sterically hindered, have from inhibition, the two key fluorescence dyes that simultaneously add can destroy the polymerizing condition of blank polymeric microspheres stabilize originally, are difficult for forming the polystyrene copolymerization fluorescent microsphere of uniform particle diameter.
Summary of the invention
The object of this invention is to provide a kind of good monodispersity that has, the preparation method of the near ultraviolet polystyrene copolymerization fluorescent microsphere with functional group that fluorescence property is good and stable.
The preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere provided by the invention is that to take vinylbenzene and N-allyl group carbazole be raw material, and copolymerization obtains fluorescent microsphere, then introduces respectively sulfonic group or oh group at microsphere surface.
Its concrete preparation method comprises the following steps:
1), initiator Diisopropyl azodicarboxylate, polyethylene of dispersing agent pyrrolidone are placed in to dehydrated alcohol and obtain clear solution, in solution, add vinylbenzene and N-allyl group carbazole simultaneously, under nitrogen protection, in 65～70 ℃ of copolyreaction 12～24h, obtain polystyrene copolymerization fluorescent microsphere; Wherein, the add-on of described N-allyl group carbazole is 0.4～0.9% of vinylbenzene quality.
2), get above-mentioned polystyrene copolymerization fluorescent microsphere and be scattered in the vitriol oil, 40～60 ℃ of reaction 6～8h, are washed to neutrality, the dry functional near ultraviolet polystyrene copolymerization of the sulfonic group fluorescent microsphere that obtains; Or above-mentioned polystyrene copolymerization fluorescent microsphere, Diisopropyl azodicarboxylate and γ-methacryloxypropyl trimethoxy silane are scattered in aqueous ethanolic solution; 65～70 ℃ of reaction 24～36h under nitrogen protection, the dry hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere that obtains of washing.
Above-mentioned steps 1), in copolyreaction, the consumption of described initiator Diisopropyl azodicarboxylate is 1.8～2.3% of vinylbenzene quality; The consumption of polyethylene of dispersing agent pyrrolidone is 4.6～6.2% of vinylbenzene quality.
Above-mentioned steps 2) in the preparation of middle hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere, the consumption of described γ-methacryloxypropyl trimethoxy silane is 2～7 times of polystyrene copolymerization fluorescent microsphere quality, the consumption of Diisopropyl azodicarboxylate is 0.24～0.48 times of polystyrene copolymerization fluorescent microsphere quality, and described aqueous ethanolic solution preferred alcohol and water are according to the solution being mixed to get at 5: 1.
The polystyrene copolymerization fluorescent microsphere that the present invention obtains above-mentioned preparation method is successively used deionized water and ethanol repetitive scrubbing, until washings no longer detects after fluorescence, in 50 ℃ of vacuum-dryings, obtains final fluorescent microsphere product.And adopt to use the same method and wash and dry sulfonic group or the hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere of obtaining.
The present invention adopts diffuse-aggregate method, vinylbenzene and two kinds of monomers of N-allyl group carbazole with two keys are carried out to copolymerization, prepared polystyrene copolymerization fluorescent microsphere, and and then to its modifying surface, obtained functional near ultraviolet polystyrene copolymerization fluorescent microsphere.
Compared with prior art, the present invention has the following advantages: the fluorescence dye N-allyl group carbazole that 1, the present invention adopts is a kind of near ultraviolet or mazarine fluorescence material, for multi-wavelength fluorometric analyses such as comprising immunofluorescence, nucleic acid and protein chip, should be used for saying, this short wavelength's blue light can provide contrasting colour, can be easier to distinguish from the fluorescent probe of other long wave transmitting.2, adopt allyl group carbazole and vinylbenzene direct polymerization, fluorescence dye is evenly distributed in microballoon, thereby has guaranteed higher microballoon whole lighting efficiency, fluorescence dye stable existence, in microballoon, is not easy leakage, cancellation, can not cause test sample contamination.3, adopt dispersion copolymerization method to prepare fluorescent microsphere, the fluorescent microsphere uniform particle diameter of preparing (2.5 μ m left and right), there is good monodispersity.4, by two kinds of approach, on fluorescent microsphere surface, introduce sulfonic group or hydroxyl functional group, particularly by microsphere surface residual double bonds, adopt polymerization to be combined with silane coupling agent KH570, thereby introduce silicon hydroxyl at microsphere surface, can be used as a kind of platform, then introduce multiple can with other specific function group of biomolecules combination.5, the present invention adopts one kettle way to prepare fluorescent microsphere, makes to prepare the easy and simple to handle of fluorescent microsphere.6, the present invention uses micro-reaction to prepare fluorescent microsphere, makes to prepare the with low cost of fluorescent microsphere.
Near ultraviolet polystyrene copolymerization fluorescent microsphere prepared by the present invention has high-quantum efficiency and wide spectral range, can be applicable to immune labeled context of detection, for follow-up biological combination.
Accompanying drawing explanation
Fig. 1 is the fluorescence emission spectrogram of the polystyrene copolymerization fluorescent microsphere that provides of the embodiment of the present invention 1.
Fig. 2 is the infrared absorption spectrum comparison diagram of the polystyrene microsphere that provides of the functional near ultraviolet polystyrene copolymerization of sulfonic group fluorescent microsphere, polystyrene copolymerization fluorescent microsphere and comparative example 1 that the embodiment of the present invention 1 provides.
Fig. 3 is the infrared absorption spectrum comparison diagram of the polystyrene microsphere that provides of hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere, polystyrene copolymerization fluorescent microsphere and comparative example 1 that embodiment 2 provides.
Fig. 4 is the SEM figure of the blank microballoon that provides of comparative example 1 and embodiment 1 and fluorescent microsphere.
Fig. 5 is the shows fluorescent microscopy images of the fluorescent microsphere that provides of embodiment 1 and embodiment 2.
Embodiment
Below in conjunction with drawings and Examples, technical solution of the present invention is further elaborated.
Embodiment 1
Get respectively Diisopropyl azodicarboxylate 0.024g, polyvinylpyrrolidone 0.072g, dehydrated alcohol 9.0g is placed in microscale reactor, ultrasonic clear solution.Add vinylbenzene 1.2g, N-allyl group carbazole 7.2mg, letting nitrogen in and deoxidizing, sealing.Be placed in constant temperature oscillator, at 70 ℃, under 130rpm condition, react 12h.Priority water and ethanol repetitive scrubbing thus obtained microsphere, until washings no longer detects after fluorescence, 50 ℃ of vacuum-dryings obtain polystyrene copolymerization fluorescent microsphere.
Take above-mentioned polystyrene copolymerization fluorescent microsphere 1.0g, add vitriol oil 28mL, the ultrasonic fluorescent microsphere that makes disperses in the vitriol oil, at 55 ℃, react 7h, wash sample with water to neutral, 50 ℃ of vacuum-dryings, obtain the functional near ultraviolet polystyrene copolymerization of sulfonic group fluorescent microsphere.
Embodiment 2
Get respectively Diisopropyl azodicarboxylate 0.028g, polyvinylpyrrolidone 0.067g, dehydrated alcohol 9.8g is placed in microscale reactor, ultrasonic clear solution.Add vinylbenzene 1.4g, N-allyl group carbazole 11.6mg, letting nitrogen in and deoxidizing, sealing.Be placed in constant temperature oscillator, at 70 ℃, under 130rpm condition, react 12h.Priority water and ethanol repetitive scrubbing thus obtained microsphere, until washings no longer detects after fluorescence, 50 ℃ of vacuum-dryings obtain polystyrene copolymerization fluorescent microsphere.
Take 0.064g AIBN, be scattered in the mixed solvent of 25.6mL ethanol/deionized water (5: 1).Take the above-mentioned polystyrene copolymerization fluorescent microsphere of 0.2g and join in mixed solvent, ultrasonic dispersion.Letting nitrogen in and deoxidizing adds gamma-methyl allyl acyloxypropyl trimethoxysilane 0.832g simultaneously, seals and shakes up.Be placed in constant temperature oscillator, 70 ℃ of violent jolting 30h.After reaction finishes, with deionized water and ethanol repetitive scrubbing centrifugal, 50 ℃ of vacuum-dryings, obtain hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere.
Comparative example 1
Get respectively Diisopropyl azodicarboxylate 0.023g, polyvinylpyrrolidone 0.066g, dehydrated alcohol 9.5g is placed in microscale reactor, ultrasonic clear solution.Add vinylbenzene 1.2g, letting nitrogen in and deoxidizing, sealing.Be placed in constant temperature oscillator, at 70 ℃, under 130rpm condition, react 12h.Successively, with second alcohol and water repetitive scrubbing thus obtained microsphere, 50 ℃ of vacuum-dryings obtain polystyrene microsphere.
Fig. 1 is the fluorescence emission spectrogram of the polystyrene copolymerization fluorescent microsphere (3% is scattered in ethanol) of embodiment 1 preparation, and as can be seen from Figure, under 328nm exciting light, the maximum emission wavelength of fluorescent microsphere is 365nm.
In Fig. 2, curve a is the infrared absorption spectrum of the functional near ultraviolet polystyrene copolymerization of the sulfonic group fluorescent microsphere of embodiment 1 preparation, curve b is the infrared absorption spectrum of the polystyrene copolymerization fluorescent microsphere of embodiment 1 preparation, and curve c is the infrared absorption spectrum of the polystyrene microsphere of comparative example 1 preparation.By curve a, can be observed 3446cm
-1it is the stretching vibration characteristic peak of O-H in sulfonic group; 3026cm
-1the stretching vibration absorption peak that one group of obvious absorption peaks of left and right is C-H on phenyl ring; 1169cm
-1, 1070cm
-1, 620cm
-1near have sulfonic charateristic avsorption band, prove sulfonic existence in sample; At fingerprint region 835cm
-1the absorption peak that has the last one, illustrate on phenyl ring, have di-substituted, 530cm
-1near absorption peak have obvious reinforcement, above-mentioned absorption peak illustrated effectively sulfonic group with phenyl ring generation chemical reaction, be bonded on polystyrene copolymerization fluorescent microsphere.Comparison curves b and c, the two is substantially the same, and this is that infrared spectrum can not symbolize the characteristic peak of fluorescence dye because fluorescence dye content in fluorescent microsphere is few.
In Fig. 3, curve a is the infrared absorption spectrum of the hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere of embodiment 2 preparations, curve b is the infrared absorption spectrum of the polystyrene copolymerization fluorescent microsphere of embodiment 2 preparations, and curve c is the infrared absorption spectrum of the polystyrene microsphere of comparative example 1 preparation.By curve a, can be observed 3026cm
-1the one group of stretching vibration absorption peak that obvious absorption peak is C-H on phenyl ring in left and right; At 3458-3311cm
-1having a wide and weak peak, is the stretching vibration characteristic peak of O-H; 2924 and 2840cm
-1absorption peak be-CH
2stretching vibration characteristic absorbance; 1236-1182cm
-1belong to Si-C, Si-O stretching vibration, these variations have absolutely proved on microsphere surface bonding gamma-methyl allyl acyloxypropyl trimethoxysilane (KH570).Comparison curves b and c, the two is substantially the same, and this is that infrared spectrum can not symbolize the characteristic peak of fluorescence dye because fluorescence dye content in fluorescent microsphere is few.
In Fig. 4, A is the blank microballoon that comparative example 1 provides, and as seen from the figure, the blank microballoon of preparation has good sphericity and monodispersity is good, for preparation copolymerization fluorescent microsphere is laid a good foundation.Fig. 4 B is the fluorescent microsphere that embodiment 1 provides, and by figure A and B, can be found out, copolymerization fluorescent microsphere and blank microballoon have essentially identical particle diameter and dispersiveness.
In Fig. 5, A and B are respectively the shows fluorescent microscopy images of the copolymerization fluorescent microsphere that provides of embodiment 1 and embodiment 2.As seen from the figure, fluorescent microsphere has uniform luminescence feature, good sphericity and monodispersity, show N-allyl group carbazole and styrene copolymerized evenly, good stability.
Claims (6)
1. a preparation method near ultraviolet polystyrene copolymerization fluorescent microsphere, is to obtain fluorescent microsphere by vinylbenzene and the copolymerization of N-allyl group carbazole, then introduces respectively sulfonic group or oh group at microsphere surface, it is characterized in that adopting following method to prepare:
1), initiator Diisopropyl azodicarboxylate, polyethylene of dispersing agent pyrrolidone are placed in to dehydrated alcohol and obtain clear solution, in solution, add vinylbenzene and N-allyl group carbazole simultaneously, under nitrogen protection, in 65～70 ℃ of copolyreaction 12～24h, obtain polystyrene copolymerization fluorescent microsphere; Wherein, the add-on of described N-allyl group carbazole is 0.4～0.9% of vinylbenzene quality;
2), get above-mentioned polystyrene copolymerization fluorescent microsphere and be scattered in the vitriol oil, 40～60 ℃ of reaction 6～8h, are washed to neutrality, the dry functional near ultraviolet polystyrene copolymerization of the sulfonic group fluorescent microsphere that obtains; Or above-mentioned polystyrene copolymerization fluorescent microsphere, Diisopropyl azodicarboxylate and γ-methacryloxypropyl trimethoxy silane are scattered in aqueous ethanolic solution; 65～70 ℃ of reaction 24～36h under nitrogen protection, the dry hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere that obtains of washing.
2. the preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere according to claim 1, is characterized in that described step 1) copolyreaction in, the consumption of initiator Diisopropyl azodicarboxylate is 1.8～2.3% of vinylbenzene quality.
3. the preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere according to claim 1, is characterized in that described step 1) copolyreaction in, the consumption of polyethylene of dispersing agent pyrrolidone is 4.6～6.2% of vinylbenzene quality.
4. the preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere according to claim 1, it is characterized in that described step 2) in the preparation of hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere, the consumption of described γ-methacryloxypropyl trimethoxy silane is 2～7 times of polystyrene copolymerization fluorescent microsphere quality.
5. the preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere according to claim 1, it is characterized in that described step 2) in the preparation of hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere, the consumption of Diisopropyl azodicarboxylate is 0.24～0.48 times of polystyrene copolymerization fluorescent microsphere quality.
6. the preparation method of near ultraviolet polystyrene copolymerization fluorescent microsphere according to claim 1, it is characterized in that described step 2) in the preparation of hydroxy-functional near ultraviolet polystyrene copolymerization fluorescent microsphere, the described aqueous ethanolic solution solution that to be ethanol and water be mixed to get according to the volume ratio of 5: 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153331A (en) * | 2015-10-16 | 2015-12-16 | 中国石油大学(华东) | Method and product for preparing autofluorescence polystyrene materials |
| CN108084316A (en) * | 2017-12-15 | 2018-05-29 | 中北大学 | A kind of preparation method of carboxylated porous crosslinked polystyrene copolymerization fluorescent microsphere |
| CN111074363A (en) * | 2019-12-30 | 2020-04-28 | 常熟涤纶有限公司 | Superfine polyester fluorescent fiber with high adsorption function and preparation method thereof |
| CN111088552A (en) * | 2019-12-30 | 2020-05-01 | 常熟涤纶有限公司 | Cationic dyeable polyester fiber and preparation method thereof |
| CN114230697A (en) * | 2021-12-06 | 2022-03-25 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Aggregation-induced emission micron-sized crosslinked polystyrene microsphere and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1264715A (en) * | 2000-03-24 | 2000-08-30 | 吉林大学 | Conjugated/unconjugated copolymer and its preparing process and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1264715A (en) * | 2000-03-24 | 2000-08-30 | 吉林大学 | Conjugated/unconjugated copolymer and its preparing process and application |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105153331A (en) * | 2015-10-16 | 2015-12-16 | 中国石油大学(华东) | Method and product for preparing autofluorescence polystyrene materials |
| CN105153331B (en) * | 2015-10-16 | 2018-04-17 | 中国石油大学(华东) | A kind of method and product for preparing autofluorescence polystyrene material |
| CN108084316A (en) * | 2017-12-15 | 2018-05-29 | 中北大学 | A kind of preparation method of carboxylated porous crosslinked polystyrene copolymerization fluorescent microsphere |
| CN111074363A (en) * | 2019-12-30 | 2020-04-28 | 常熟涤纶有限公司 | Superfine polyester fluorescent fiber with high adsorption function and preparation method thereof |
| CN111088552A (en) * | 2019-12-30 | 2020-05-01 | 常熟涤纶有限公司 | Cationic dyeable polyester fiber and preparation method thereof |
| CN111074363B (en) * | 2019-12-30 | 2022-04-15 | 常熟涤纶有限公司 | Superfine polyester fluorescent fiber with high adsorption function and preparation method thereof |
| CN114230697A (en) * | 2021-12-06 | 2022-03-25 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Aggregation-induced emission micron-sized crosslinked polystyrene microsphere and preparation method and application thereof |
| CN114230697B (en) * | 2021-12-06 | 2023-12-26 | 广东省大湾区华南理工大学聚集诱导发光高等研究院 | Aggregation-induced emission micron-sized crosslinked polystyrene microsphere and preparation method and application thereof |
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