CN103725017A - Fish collagen antibacterial film with slow release effect and preparation method thereof - Google Patents
Fish collagen antibacterial film with slow release effect and preparation method thereof Download PDFInfo
- Publication number
- CN103725017A CN103725017A CN201310721581.3A CN201310721581A CN103725017A CN 103725017 A CN103725017 A CN 103725017A CN 201310721581 A CN201310721581 A CN 201310721581A CN 103725017 A CN103725017 A CN 103725017A
- Authority
- CN
- China
- Prior art keywords
- film
- essential oil
- cinnamon essential
- nanometer liposome
- isin glue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
- General Preparation And Processing Of Foods (AREA)
Abstract
The invention discloses a fish collagen antibacterial film with a slow release effect and a preparation method thereof, belonging to the technical field of slow release and bacterial resistance applications of nanoliposomes. The preparation method comprises the following steps: dissolving fish collagen and glycerin in water to prepare a fish collagen solution, adding a cinnamon essential oil nanoliposome suspension solution, uniformly stirring, ultrasonically degassing, moving and spreading on an organic glass plate, and drying to obtain the film. Compared with a simple fish collagen film, the antibacterial film prepared by the method is greatly improved in antibacterial performance; due to a protection effect of lipidosomes on cinnamon essential oil and a slow release effect of the cinnamon essential oil, the antibacterial performance of the composite film can be kept for a long time. In addition, the film can be biodegradable, cannot pollute an environment in a using process, has the functions of preventing flavor substances of foods from being lost, preventing the foods from being polluted and oxidized and the like, and achieves the effects of preserving the foods and prolonging the shelf life.
Description
Technical field
The invention belongs to nanometer liposome slowly-releasing and antibacterial applied technical field, be specifically related to a kind of Isin glue collagen antibacterial film with slow releasing function and preparation method thereof.
Background technology
Collagen protein is a kind of important natural macromolecular material, antigenicity is low, has good biocompatibility and biodegradability, therefore, as natural biomass resource, importance and the economic status in the industry such as food, biomedicine, makeup, applied become increasingly conspicuous.But simple collagen film physicochemical property relatively a little less than, in order to improve the character of film, can before film forming, add Cucumber in collagen solution, for example, can add antibacterial ability or the antioxidant property with bioactive natural product raising collagen film; Or add as required the mechanical property of polysaccharide or lipid change collagen film, permeability etc.But because essential oil itself has certain volatility and adds rayed, oxygen and other factors affect that itself is easily oxidized, decompose and destroy, and it can not be played a role for a long time.In order to extend the anti-microbial activity of film, can adopt the biologically active substance to adding first to add protection, make it in use maintain long activity.The present invention has adopted and active substance is made to nanometer liposome form has first added protection and add in collagen solution again, makes cinnamon essential oil nanometer liposome collagen film, has controlled the release of cinnamon essential oil, has extended the germ resistance of film.
Summary of the invention
The object of the present invention is to provide a kind of Isin glue collagen antibacterial film with slow releasing function and preparation method thereof; the antibacterial film that the present invention makes anti-microbial property compared with simple collagen film is greatly improved; and due to the provide protection of liposome to cinnamon essential oil and the slowly-releasing of cinnamon essential oil, make the sustainable long period of anti-microbial property of composite membrane.In addition, this film can be biodegradable, can be to environment in use procedure, and have and prevent that the flavour substances of food scatters and disappears, prevent the functions such as food is contaminated and oxidized, reach the effect of food fresh keeping and Shelf-life.
For achieving the above object, the present invention adopts following technical scheme:
In a kind of component of the Isin glue collagen antibacterial film with slow releasing function, contain cinnamon essential oil nanometer liposome, Isin glue collagen and glycerine.
The method of preparing the Isin glue collagen antibacterial film with slow releasing function as above comprises the following steps:
(1) prepare cinnamon essential oil nanometer liposome suspension altogether
Take soybean lecithin 200mg, cinnamon essential oil 100-140mg, cholesterol 25mg, VE1.5mg and Sodium cholic acid 2mg and be dissolved in the chloroform that volume is 60ml, decompression rotary evaporation is removed organic solvent, makes lipid in flask walls, form film; Then in flask, add the phosphoric acid buffer 60ml of pH=7.0 to form suspension, at 40 ℃ of water-bath rotary evaporation in vacuo, it is mixed suspension; The last tween-80 that adds 300ul in suspension, through Probe Ultrasonic Searching processing: 100W, 2s opens, and 2s stops, and ultrasonic 6min obtains cinnamon essential oil nanometer liposome suspension altogether;
(2) prepare Isin glue collagen solution
2-3g Isin glue collagen is placed in to 100ml round-bottomed flask, with at room temperature swelling 2h of 50ml deionized water, adds 0.5-0.75g glycerine as softening agent, then stirring and dissolving 1h at 45 ℃, mixes it, makes Isin glue collagen solution;
(3) the antibiosis rubber collagen film that preparation contains cinnamon essential oil nanometer liposome
Cinnamon essential oil nanometer liposome prepared by step (1) altogether suspension 50ml joins in the Isin glue collagen solution of 50ml prepared by step (2), stirs, and ultrasonic degas, 45 ℃ of insulations; Pipette the Isin glue collagen solution that 10ml added cinnamon essential oil nanometer liposome at every turn and spread into film forming on poly (methyl methacrylate) plate, film-forming temperature is 45 ℃, and film formation time is 48h, makes the antibiosis rubber collagen film that contains cinnamon essential oil nanometer liposome.
The analytical procedure that the present invention adopts:
(1) cinnamon essential oil nanometer liposome median size: with ZETASIZER 3000 laser nano particle size analyzer determinations.
(2) cinnamon essential oil nanometer liposome encapsulation rate: by UV-2450 Zi Wai – visible spectrophotometer mensuration.
(3) Zeta potential: by ZataSizer2000 type Zeta potential determinator mensuration.
(4) release experiment: survey its burst size with Agilent 1100 high performance liquid chromatography.What in figure, ■ represented is the collagen film that contains cinnamon essential oil solution, and zero expression is the collagen film that is added with cinnamon essential oil nanometer liposome.
(5) antibacterial stability: measure with disc diffusion method.
Beneficial effect of the present invention is: the present invention adopts nanometer liposome slowly-releasing and antibacterial edible film technology to prepare the antibiosis rubber collagen film of fixing cinnamon essential oil nanometer liposome, control the release of cinnamon essential oil in collagen film, extended the antibacterial effect of collagen film.
Accompanying drawing explanation
Fig. 1 is cinnamon essential oil nanometer liposome antibiosis rubber collagen film and the release graphics of the collagen film that contrasts cinnamon essential oil under 4 degree.
Fig. 2 is that the antibiosis rubber collagen film that contains cinnamon essential oil nanometer liposome and the collagen film that contrasts cinnamon essential oil are respectively 3 days and the inhibition colibacillary ability comparison of storage after 30 days.The collagen film of a, contrast cinnamon essential oil, the antibiosis rubber collagen film of b, cinnamon essential oil nanometer liposome.
Embodiment
A kind of preparation method of the Isin glue collagen antibacterial film with slow releasing function comprises the following steps:
(1) prepare cinnamon essential oil nanometer liposome suspension altogether
Take soybean lecithin 200mg, cinnamon essential oil 100-140mg, cholesterol 25mg, VE1.5mg and Sodium cholic acid 2mg and be dissolved in the chloroform that volume is 60ml, decompression rotary evaporation is removed organic solvent, makes lipid in flask walls, form film; Then in flask, add the phosphoric acid buffer 60ml of pH=7.0 to form suspension, at 40 ℃ of water-bath rotary evaporation in vacuo, it is mixed suspension; The last tween-80 that adds 300ul in suspension, through Probe Ultrasonic Searching processing: 100W, 2s opens, and 2s stops, and ultrasonic 6min obtains cinnamon essential oil nanometer liposome suspension altogether;
(2) prepare Isin glue collagen solution
2-3g Isin glue collagen is placed in to 100ml round-bottomed flask, with at room temperature swelling 2h of 50ml deionized water, adds 0.5-0.75g glycerine as softening agent, then stirring and dissolving 1h at 45 ℃, mixes it, makes Isin glue collagen solution;
(3) the antibiosis rubber collagen film that preparation contains cinnamon essential oil nanometer liposome
Cinnamon essential oil nanometer liposome prepared by step (1) altogether suspension 50ml joins in the Isin glue collagen solution of 50ml prepared by step (2), stirs, and ultrasonic degas, 45 ℃ of insulations; Pipette the Isin glue collagen solution that 10ml added cinnamon essential oil nanometer liposome at every turn and spread into film forming on poly (methyl methacrylate) plate, film-forming temperature is 45 ℃, and film formation time is 48h, makes the antibiosis rubber collagen film that contains cinnamon essential oil nanometer liposome.
Take soybean lecithin 200mg, cinnamon essential oil 100mg, cholesterol 25mg, VE1.5mg, Sodium cholic acid 2mg is dissolved in the chloroformic solution that volume is 60ml, and decompression rotary evaporation is removed organic solvent, makes lipid in flask walls, form film; Then in flask, add the phosphoric acid buffer 60ml of pH=7.0 to form suspension, at 40 ℃ of water-bath rotary evaporation in vacuo, it is mixed suspension; The last tween-80 that adds 300ul in suspension, and by it at ice-water bath Probe Ultrasonic Searching (100W, 2s stops, 2s opens) 6min, get final product to obtain cinnamon essential oil nanometer liposome.
2g collagen protein is placed in to 100ml beaker, with at room temperature swelling 2h of 50ml deionized water, adds 0.5g glycerine as softening agent, then stirring and dissolving 1h at 45 ℃, mixes it, makes collagen solution.
Cinnamon essential oil nanometer liposome is total to suspension 50ml and joins in the gelatin solution of 50ml, stir, ultrasonic degas, 45 ℃ of insulations; Pipette the collagen solution that 10ml added cinnamon essential oil nanometer liposome at every turn and spread into film forming on poly (methyl methacrylate) plate, film-forming temperature is 45 ℃, and film formation time is 48h.
embodiment 2
Take soybean lecithin 200mg, cinnamon essential oil 140mg, cholesterol 25mg, VE1.5mg, Sodium cholic acid 2mg is dissolved in the chloroformic solution that volume is 60ml, and decompression rotary evaporation is removed organic solvent, makes lipid in flask walls, form film; Then in flask, add the phosphoric acid buffer 60ml of pH=7.0 to form suspension, at 40 ℃ of water-bath rotary evaporation in vacuo, it is mixed suspension; The last tween-80 that adds 300ul in suspension, and by it at ice-water bath Probe Ultrasonic Searching (100W, 2s stops, 2s opens) 6min, get final product to obtain cinnamon essential oil nanometer liposome.
3g collagen protein is placed in to 100ml beaker, with at room temperature swelling 2h of 50ml deionized water, adds 0.75g glycerine as softening agent, then stirring and dissolving 1h at 45 ℃, mixes it, makes collagen solution.
Cinnamon essential oil nanometer liposome is total to suspension 50ml and joins in the gelatin solution of 50ml, stir, ultrasonic degas, 45 ℃ of insulations; Pipette the collagen solution that 10ml added cinnamon essential oil nanometer liposome at every turn and spread into film forming on poly (methyl methacrylate) plate, film-forming temperature is 45 ℃, and film formation time is 48h.
From Fig. 1, we can find out, compared with cinnamon essential oil is made and is joined after nanometer liposome and directly to join in collagem membrane with cinnamon essential oil solution in collagem membrane, the former release rate has reduced a lot, reach the effect of slowly-releasing, therefore, thus can extend its shelf-lives by more lasting fresh-keeping foodstuff with this film wrap food.
As can be seen from Figure 2, at the beginning in good initial period of this film preparation (the 3rd day), this film, to colibacillary inhibition, contains and contrasts compared with the collagen film of cinnamon essential oil and the antibiosis rubber collagen film of cinnamon essential oil nanometer liposome, shows slightly high antibacterial ability.But along with the prolongation in storage time; the antibacterial ability of the latter's film is on the contrary higher than the former; this be mainly because the bilayer structural defence of nanometer liposome main antimicrobial component phenylacrolein in cinnamon essential oil be not subject to external interference; and this structure has also extended the residence time in cinnamon essential oil bilayer; active ingredient phenylacrolein in cinnamon essential oil can be slowly released in time; reach the effect of slowly-releasing, thereby increase the antibacterial stability of liposome collagen film.Visible cinnamon essential oil is made after nanometer liposome, along with the prolongation of time, the germ resistance that can not only improve this film has also extended the shelf-lives of food simultaneously.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. an Isin glue collagen antibacterial film with slow releasing function, is characterized in that: in described membrane component, contain cinnamon essential oil nanometer liposome, Isin glue collagen and glycerine.
2. a method of preparing the Isin glue collagen antibacterial film with slow releasing function as claimed in claim 1, is characterized in that: comprise the following steps:
(1) prepare cinnamon essential oil nanometer liposome suspension altogether
Take soybean lecithin 200mg, cinnamon essential oil 100-140mg, cholesterol 25mg, VE1.5mg and Sodium cholic acid 2mg and be dissolved in the chloroform that volume is 60ml, decompression rotary evaporation is removed organic solvent, makes lipid in flask walls, form film; Then in flask, add the phosphoric acid buffer 60ml of pH=7.0 to form suspension, at 40 ℃ of water-bath rotary evaporation in vacuo, it is mixed suspension; The last tween-80 that adds 300ul in suspension, through Probe Ultrasonic Searching processing: 100W, 2s opens, and 2s stops, and ultrasonic 6min obtains cinnamon essential oil nanometer liposome suspension altogether;
(2) prepare Isin glue collagen solution
2-3g Isin glue collagen is placed in to 100ml round-bottomed flask, with at room temperature swelling 2h of 50ml deionized water, adds 0.5-0.75g glycerine as softening agent, then stirring and dissolving 1h at 45 ℃, mixes it, makes Isin glue collagen solution;
(3) the antibiosis rubber collagen film that preparation contains cinnamon essential oil nanometer liposome
Cinnamon essential oil nanometer liposome prepared by step (1) altogether suspension 50ml joins in the Isin glue collagen solution of 50ml prepared by step (2), stirs, and ultrasonic degas, 45 ℃ of insulations; Pipette the Isin glue collagen solution that 10ml added cinnamon essential oil nanometer liposome at every turn and spread into film forming on poly (methyl methacrylate) plate, film-forming temperature is 45 ℃, and film formation time is 48h, makes the antibiosis rubber collagen film that contains cinnamon essential oil nanometer liposome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310721581.3A CN103725017B (en) | 2013-12-25 | 2013-12-25 | A kind of fish collagen antibacterial film with slow releasing function and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310721581.3A CN103725017B (en) | 2013-12-25 | 2013-12-25 | A kind of fish collagen antibacterial film with slow releasing function and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103725017A true CN103725017A (en) | 2014-04-16 |
| CN103725017B CN103725017B (en) | 2016-03-30 |
Family
ID=50449327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310721581.3A Active CN103725017B (en) | 2013-12-25 | 2013-12-25 | A kind of fish collagen antibacterial film with slow releasing function and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103725017B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164090A (en) * | 2014-05-30 | 2014-11-26 | 中国计量学院 | EGCG nano liposome edible film and preparation method thereof |
| CN104382039A (en) * | 2014-12-19 | 2015-03-04 | 福州大学 | Nano lipidosome containing green tea extractive, and preparation method thereof |
| CN104387779A (en) * | 2014-11-05 | 2015-03-04 | 福州大学 | Antioxidative collagen film containing active plant extract nanoliposome |
| CN106317909A (en) * | 2016-08-25 | 2017-01-11 | 董晓 | Preparing method of antibacterial medical grade thin film material |
| CN112021393A (en) * | 2019-06-03 | 2020-12-04 | 浙江工商大学 | Composite preservative containing natural essential oil nanoliposome and application of composite preservative in sturgeon fillet preservation |
| CN114698676A (en) * | 2022-03-23 | 2022-07-05 | 福州大学 | Preparation method and application of silver carp skin collagen-based antibacterial and antioxidant active coating |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461534A (en) * | 2009-01-14 | 2009-06-24 | 江南大学 | Method for preparing antioxidation gelatine membrane containing tea polyphenol nano lipidosome |
| US20100178329A1 (en) * | 2007-06-19 | 2010-07-15 | Gaal Jozsef | Liposome composition |
| CN103030979A (en) * | 2012-12-27 | 2013-04-10 | 福建省博特生物科技有限公司 | Collagen antibacterial membrane containing cinnamon essential oil and preparation method of collagen antibacterial membrane |
-
2013
- 2013-12-25 CN CN201310721581.3A patent/CN103725017B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100178329A1 (en) * | 2007-06-19 | 2010-07-15 | Gaal Jozsef | Liposome composition |
| CN101461534A (en) * | 2009-01-14 | 2009-06-24 | 江南大学 | Method for preparing antioxidation gelatine membrane containing tea polyphenol nano lipidosome |
| CN103030979A (en) * | 2012-12-27 | 2013-04-10 | 福建省博特生物科技有限公司 | Collagen antibacterial membrane containing cinnamon essential oil and preparation method of collagen antibacterial membrane |
Non-Patent Citations (2)
| Title |
|---|
| 张媛: "《中药制剂技术（第1版）》", 31 August 2012, 河南科学技术出版社 * |
| 王晓波: "《药物运释系统（第1版）》", 31 August 2007, 中国医药科技出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164090A (en) * | 2014-05-30 | 2014-11-26 | 中国计量学院 | EGCG nano liposome edible film and preparation method thereof |
| CN104387779A (en) * | 2014-11-05 | 2015-03-04 | 福州大学 | Antioxidative collagen film containing active plant extract nanoliposome |
| CN104382039A (en) * | 2014-12-19 | 2015-03-04 | 福州大学 | Nano lipidosome containing green tea extractive, and preparation method thereof |
| CN106317909A (en) * | 2016-08-25 | 2017-01-11 | 董晓 | Preparing method of antibacterial medical grade thin film material |
| CN112021393A (en) * | 2019-06-03 | 2020-12-04 | 浙江工商大学 | Composite preservative containing natural essential oil nanoliposome and application of composite preservative in sturgeon fillet preservation |
| CN114698676A (en) * | 2022-03-23 | 2022-07-05 | 福州大学 | Preparation method and application of silver carp skin collagen-based antibacterial and antioxidant active coating |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103725017B (en) | 2016-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103725017B (en) | A kind of fish collagen antibacterial film with slow releasing function and preparation method thereof | |
| CN101461535B (en) | Method for preparing antioxidation gelatine membrane containing tea polyphenol-chitosan nano grain | |
| CN101461534B (en) | Method for preparing antioxidation gelatine membrane containing tea polyphenol nano lipidosome | |
| CN109566954B (en) | Laurus essential oil and nano-silver embedded liposome/chitosan antibacterial and antioxidant coating solution and preparation method and application thereof | |
| Fabra et al. | Use of the electrohydrodynamic process to develop active/bioactive bilayer films for food packaging applications | |
| AU2013333184B2 (en) | Hyaluronic acid gel and method for producing same | |
| Trovatti et al. | Bacterial cellulose membranes applied in topical and transdermal delivery of lidocaine hydrochloride and ibuprofen: in vitro diffusion studies | |
| Dupuis et al. | Nanodelivery of essential oils as efficient tools against antimicrobial resistance: A review of the type and physical-chemical properties of the delivery systems and applications | |
| JP6598207B2 (en) | Water-soluble hyaluronic acid gel and method for producing the same | |
| David et al. | Formulation and in vitro evaluation of ethosomes as vesicular carrier for enhanced topical delivery of isotretinoin | |
| Tan et al. | Polyelectrolyte microcapsules built on CaCO3 scaffolds for the integration, encapsulation, and controlled release of copigmented anthocyanins | |
| Badawy et al. | Preparation and characterization of biopolymers chitosan/alginate/gelatin gel spheres crosslinked by glutaraldehyde | |
| BR112014026935A2 (en) | delayed release drug formulation and method for producing a delayed release drug formulation | |
| NZ591660A (en) | Solid oil powders | |
| CN103520007A (en) | Skin active factor flexible nano-liposome and preparation method and application thereof | |
| Tan et al. | Yeast cell-derived delivery systems for bioactives | |
| CN106389137A (en) | Flaxseed oil liposome and preparation and applications thereof | |
| CN105456193A (en) | EGCC liposome preparation and preparation method thereof | |
| Abraham et al. | Release of encapsulated bioactives influenced by alginate viscosity under in-vitro gastrointestinal model | |
| CN104689771A (en) | Preparation method of oxyresveratrol microcapsules and products thereof | |
| Babayev et al. | Antimicrobial pseudolatex zein films with encapsulated carvacrol for sustainable food packaging | |
| Khuntia et al. | Release behavior of vitamin C nanoliposomes from starch–vitamin C active packaging films | |
| Manna et al. | Recent update on alginate based promising transdermal drug delivery systems | |
| Kim et al. | Preparation, physical characterization, and in vitro skin permeation of deformable liposomes loaded with taxifolin and taxifolin tetraoctanoate | |
| CN104387779A (en) | Antioxidative collagen film containing active plant extract nanoliposome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |