CN103705554B - A kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction - Google Patents
A kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction Download PDFInfo
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- CN103705554B CN103705554B CN201310701032.XA CN201310701032A CN103705554B CN 103705554 B CN103705554 B CN 103705554B CN 201310701032 A CN201310701032 A CN 201310701032A CN 103705554 B CN103705554 B CN 103705554B
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Abstract
The invention discloses a kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction, blue or green Folium Alangii sheet drying, pulverize as extracting raw material, extract raw material in ultrasonic extraction still with ethanol for solvent, under gas-lifting type stirring condition, independent-excited ultrasonic vocalization mode, extracting mode is that interval 2min is ultrasonic once, each 3min, supersound extraction 30min obtains extracting solution, aqueous phase concentrate drying just obtains extractive of general flavone from blue or green Folium Alangii, this extracting method impurity is few, extraction ratio is high up to more than 97%, extraction time is short, more than 10 times times can be shortened than traditional extraction process, operation is simple, effective ingredient in medical material had protective effect, the physiologically active of thermal sensitivity medicine will not be destroyed, simultaneously, energy consumption can be substantially reduced.
Description
Technical field
The present invention relates to the extractive technique of flavone, be specifically related to a kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction.
Background technology
Blue or green maple belongs to Aceraceae, Acer plants, produces China, Japan and Korea；Distribution in China is in Yangtze river basin each province, and Shandong, Henan, Zhejiang also have.It is born in below height above sea level 1200m mountain region, the border on hills or sparse woods more.Flavone compound (Flavonoids) is a class low molecule Plant Secondary Metabolites, is distributed widely in veterinary antibiotics and medicinal plants.This compounds has common parent nucleus C6-C3-C6Structure, has separated from vegetable material at present and has obtained hundreds of kind flavone compound, and new compound has also been found constantly.Wherein Flavanones (flavonoids) compound is different according to substituent group and position thereof, can be classified as I aglycon-apigenin (Apigenin) be main body, II aglycon-luteolin (Luteolin) be main body, III aglycon-kaempferol (Kaempferol) be main body, IV aglycon-Quercetin (Quercetin) be main body, V naringenin (Naringin) be main body totally 5 big class.Effect of flavone compound is many-sided, and it is a kind of very strong antioxidant, can the effective oxygen-derived free radicals in purged body, it is possible to improve blood circulation, it is possible to reduce cholesterol, improve the symptom etc. of cardiovascular and cerebrovascular disease.Blue or green maple also contains flavanone compounds.
Summary of the invention
The technical problem to be solved is to provide that a kind of impurity is few, simple to operate, extraction ratio is high, extraction time is short extracts the method for total flavones in blue or green Folium Alangii based on ultrasonic assistant.
This invention address that the technical scheme that above-mentioned technical problem adopts is: a kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction, its step is as follows:
A, by fresh blue or green Folium Alangii sheet, under 60 DEG C of conditions dry 24h, then pulverize, cross 0.45 μm sieve for extract raw material, after extractions raw material is weighed loading ultrasonic extraction still in；
B, in ultrasonic extraction still add mass percentage concentration be the ethanol of 30-50 DEG C, the mass volume ratio of described extraction raw material and ethanol is 1g:14-16ml, under gas-lifting type stirring condition, at 30-45 DEG C, supersound extraction 30min obtains extracting solution, extracting solution concentrating under reduced pressure goes alcohol phase, and aqueous phase concentrate drying obtains extractive of general flavone；Wherein ultrasonic occurring mode is: independent-excited ultrasonic vocalization, radial vibration column transducer, and ultrasonic power is 300W, and supersonic frequency is 22-55kHz, and extracting mode is that interval 2min is ultrasonic once, each 3min.
Compared with prior art, it is an advantage of the current invention that a kind of based on the method for total flavones in the blue or green Folium Alangii of ultrasonic assistant extraction, blue or green Folium Alangii sheet drying, pulverize as extracting raw material, extract raw material in ultrasonic extraction still with ethanol for solvent, under gas-lifting type stirring condition, independent-excited ultrasonic vocalization mode, extracting mode is that interval 2min is ultrasonic once, each 3min, supersound extraction 30min obtains extracting solution, aqueous phase concentrate drying just obtains extractive of general flavone from blue or green Folium Alangii, this extracting method impurity is few, extraction ratio is high up to more than 97%, extraction time is short, more than 10 times times can be shortened than traditional extraction process, operation is simple, effective ingredient in medical material had protective effect, the physiologically active of thermal sensitivity medicine will not be destroyed, simultaneously, energy consumption can be substantially reduced.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
By fresh blue or green Folium Alangii sheet, at 60 DEG C, dry 24h, pulverize, cross 0.45 μm of sieve, obtain 50g and extract raw material, load in ultrasonic extraction still, add the ethanol 750mL of mass percentage concentration 40%, mixing, extracting solution is obtained in 35 DEG C of supersound extraction 30min under gas-lifting type stirring condition, extracting solution concentrating under reduced pressure goes alcohol phase, and aqueous phase concentrate drying obtains extractive of general flavone, and in extract, flavones content is about 72%.Wherein ultrasonic occurring mode: independent-excited ultrasonic vocalization, radial vibration column transducer, ultrasonic power is 300W, and supersonic frequency is 33kHz, and extracting mode is that interval 2min is ultrasonic once, each 3min.
Embodiment 2
Substantially the same manner as Example 1, different simply adds the ethanol 800mL that mass percentage concentration is 30%, and supersonic frequency is 22kHz, and in extract, flavones content is about 65%.
Embodiment 3
Substantially the same manner as Example 1, different simply adds the ethanol 700mL that mass percentage concentration is 50%, and supersonic frequency is 55kHz, and in extract, flavones content is about 70%.
In above-mentioned flavone extract, the content detection of flavone compound is according to Al3+-rutin binary complex fluorimetry is analyzed, excitation spectrum bandwidth 3nm, excitation wavelength lambda ex436nm, emission spectrum bandwidth 5nm, λ em483nm, and rutin standard solution fluorescence intensity is to respective concentration mapping quantitatively.In extracting solution, the mensuration of active constituent content adopts high-efficient liquid phase chromatogram technique analysis in 4, and chromatographic column is Varian, Microsorb-MV100C18, detects wavelength 360nm, and mobile phase is methanol/water=55: 45, and phosphoric acid adjusts pH=2.5；Column temperature is 25 DEG C, flow velocity is 1.0ml/min, sample size is 10 μ l, standard substance quantified by external standard method, in flavone extract, flavonoid content is about 65-75%, and Main Ingredients and Appearance is IV aglycon-Quercetin flavonoid monomer, such as Quercetin-3-O-β-D-galactoside, Quercetin-3-O-alpha-L-rhamnoside, Quercetin, rutin etc..
The extractive of general flavone that the present invention prepares have improve microcirculation, arrhythmia etc. effect.Can be experimental subject with SD rat during microcirculation of the heart impact is tested, give 4.0g(crude drug) extract gastric infusion every day of/kg, with matched group capacity gavages such as (7 days) normal saline venous blood sampling simultaneously after 7 days, with adenosine diphosphate sodium salt (ADP) for derivant, each group of platelet aggregation is surveyed by Born turbidimetry, and take cardiac blood simultaneously, T hrombosis inducer is formed thrombosis, result shows, administration group compares with matched group, 1min platelet aggregation rate is obviously reduced, difference statistically significant (P < 0.05), thrombosis length also has substantially shortening (P < 0.05).On in ARR impact experiment, can be experimental subject with rat, give the extract saturated solution gastric infusion of 1.0ml/kg every day, bringing out arrhythmia with matched group capacity gavages such as (8 days) normal saline Injection of Adrenaline and calcium chloride simultaneously after 8 days, result administration group has obvious antagonism Arrhythmia (P < 0.05) compared with matched group.
Claims (1)
1. one kind is extracted the method for total flavones in blue or green Folium Alangii based on ultrasonic assistant, it is characterised in that step is as follows:
A, by fresh blue or green Folium Alangii sheet, under 60 DEG C of conditions dry 24h, then pulverize, cross 0.45 μm sieve for extract raw material, after extractions raw material is weighed loading ultrasonic extraction still in；
B, in ultrasonic extraction still add mass percentage concentration be the ethanol of 30-50%, the mass volume ratio of described extraction raw material and ethanol is 1g 14-16ml, under gas-lifting type stirring condition, at 30-45 DEG C, supersound extraction 30min obtains extracting solution, extracting solution concentrating under reduced pressure goes alcohol phase, and aqueous phase concentrate drying obtains extractive of general flavone；Wherein ultrasonic occurring mode is: independent-excited ultrasonic vocalization, radial vibration column transducer, and ultrasonic power is 300W, and supersonic frequency is 22-55kHz, and extracting mode is that interval 2min is ultrasonic once, each 3min.
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| CN103705554B true CN103705554B (en) | 2016-07-06 |
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| CN104402854A (en) * | 2014-11-12 | 2015-03-11 | 重庆市畜牧科学院 | Method for extracting quercetin from mulberry leaves by utilizing ultrasonic waves |
| CN106214774A (en) * | 2016-08-30 | 2016-12-14 | 新疆医科大学 | A kind of extraction method of cassie card Pu's Fructus Jujubae total flavones |
| CN114533772B (en) * | 2022-03-01 | 2022-12-23 | 贵州两山康瑞食品检测科技有限公司 | Method for extracting and purifying total flavonoids of Acer truncatum leaves |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1844130A (en) * | 2006-04-13 | 2006-10-11 | 安徽农业大学 | Maple extract and its use in preparation of free radical scavenger or antioxidant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1844130A (en) * | 2006-04-13 | 2006-10-11 | 安徽农业大学 | Maple extract and its use in preparation of free radical scavenger or antioxidant |
Non-Patent Citations (2)
| Title |
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| 鸡爪槭黄酮提取工艺研究;王桃云等;《江苏中医药》;20061231;第27卷(第03期);50－52 * |
| 黄酮类化合物提取和纯化工艺研究进展;宋秋华等;《山西化工》;20070831;第27卷(第04期);24－27 * |
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