CN103665894A - Nanometer silk fibroin-nano-silver antibacterial composite material - Google Patents
Nanometer silk fibroin-nano-silver antibacterial composite material Download PDFInfo
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- CN103665894A CN103665894A CN201310554581.9A CN201310554581A CN103665894A CN 103665894 A CN103665894 A CN 103665894A CN 201310554581 A CN201310554581 A CN 201310554581A CN 103665894 A CN103665894 A CN 103665894A
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Abstract
The invention discloses a nanometer silk fibroin-nano-silver antibacterial composite material and a preparation method thereof. A nanometer silk fibroin membrane is used for reducing silver nitrate in situ so as to prepare the nanometer silk fibroin-nano-silver antibacterial composite material, the nanometer silk fibroin-nano-silver antibacterial composite material is washed by deionized water for five times and then is air dried at room temperature so as to obtain the nanometer silk fibroin-nano-silver antibacterial composite membrane. The nanometer silk fibroin-nano-silver antibacterial composite membrane can be widely used in fields such as food packaging, hygienic products, and medical materials.
Description
Technical field
The present invention relates to the invention belongs to nano material, anti-biotic material technical field, be specifically related to a kind of nano thread fibroin-nanometer silver antimicrobial matrix material.
Background technology
Bacterium is ubiquitous, all-pervasive, their propagation and spread the health that the mankind in serious threat.Along with a large amount of uses of the chemicalses such as microbiotic, sterilizing agent and sterilant, the bacterial dissociation population being caused by resistance is more and more, and the global bacterium Disaster Event causing thus occurs again and again.For suppressing the generation of this bacterioid disaster, research and development and application novel antibacterial material are imperative.Anti-biotic material is mainly divided into inorganic antibacterial material, organic anti-bacterial material, organic-inorganic composite antimicrobial material at present.
Along with people constantly increase the requirement for all kinds of anti-biotic materials, the exploitation of anti-biotic material has obtained unprecedented attention, the emphasis of paying close attention to and the main growth point of demand are organic polymer anti-biotic materials, now just at the antibiotic polymer material of extensive exploitation, adopt respectively the technological line that adds inorganic salt antiseptic-germicide and organic compound antiseptic-germicide, but organic synthesis antimicrobial additive all belongs to organic compound, its volatility, perviousness and transport property are strong, exist and easily lose efficacy and hygienic safety hidden danger.
Summary of the invention
For addressing the above problem, the invention discloses a kind of nano thread fibroin-nanometer silver antimicrobial matrix material.
Preparation nanometer fibroin protein film, and utilize nano thread fibroin film in-situ reducing Silver Nitrate to prepare nano thread fibroin-nanometer silver antimicrobial composite membrane, concrete steps are as follows:
(1) the nanometer silk fibroin water solution that preparation quality concentration is 2-5%, dropwise adds sodium hydrate regulator solution pH to 8 under stirring, then prepare nanometer fibroin protein film;
(2) nanometer fibroin protein film step (1) being obtained is immersed in the silver nitrate solution that concentration is 5 ~ 10 mg/mL, and soak time is 1 – 2.5h;
(3) the nanometer fibroin protein film immersed with silver nitrate solution step (2) being obtained is exposed under sunlight, and illumination action time is 1-5h; With deionized water wash 5 times, room temperature is dried; Obtain nano thread fibroin-nanometer silver antimicrobial composite membrane.
Further, the application that described nano thread fibroin-nanometer silver antimicrobial matrix material can be in food product pack, sanitary product, medical material.
Positively effect of the present invention is: without additional other chemical reagent, pollute few.Reaction conditions is gentle, without severe condition such as High Temperature High Pressure, and good energy-conserving effect.Prepared nanometer silver particle diameter little (about 5-7 nm), size distribution are even.Little particle diameter has higher specific surface area, also just means higher anti-microbial activity and lower consumption.Nanometer silver good stability, places and still can keep good dispersiveness, and can dissolve each other with plurality of reagents half a year, therefore has Application Areas widely.Nano thread fibroin is as mould material matrix, and biocompatibility and biological degradability are good.Antibacterial high efficiency, in material 2 h that silver-colored mass concentration is 1%, sterilizing rate can reach more than 99.9%.Broad-spectrum antibacterial property, prepared anti-biotic material has all shown excellent resistance for gram-positive microorganism and Gram-negative bacteria.
Embodiment
Below in conjunction with embodiment, describe technical scheme of the present invention in detail, but protection domain is not so limited.
1 one kinds of nano thread fibroin-nanometer silver antimicrobial matrix materials of embodiment.
Preparation nanometer fibroin protein film, and utilize nano thread fibroin film in-situ reducing Silver Nitrate to prepare nano thread fibroin-nanometer silver antimicrobial composite membrane, concrete steps are as follows:
(1) the nanometer silk fibroin water solution that preparation quality concentration is 2%, dropwise adds sodium hydrate regulator solution pH to 8 under stirring, then prepare nanometer fibroin protein film;
(2) nanometer fibroin protein film step (1) being obtained is immersed in the silver nitrate solution that concentration is 10 mg/mL, and soak time is 1 – 2.5h;
(3) the nanometer fibroin protein film immersed with silver nitrate solution step (2) being obtained is exposed under sunlight, and illumination action time is 5h; With deionized water wash 5 times, room temperature is dried; Obtain nano thread fibroin-nanometer silver antimicrobial composite membrane.
2 one kinds of nano thread fibroin-nanometer silver antimicrobial matrix materials of embodiment.
Preparation nanometer fibroin protein film, and utilize nano thread fibroin film in-situ reducing Silver Nitrate to prepare nano thread fibroin-nanometer silver antimicrobial composite membrane, concrete steps are as follows:
(1) the nanometer silk fibroin water solution that preparation quality concentration is 5%, dropwise adds sodium hydrate regulator solution pH to 8 under stirring, then prepare nanometer fibroin protein film;
(2) nanometer fibroin protein film step (1) being obtained is immersed in the silver nitrate solution that concentration is 10 mg/mL, and soak time is 2h;
(3) the nanometer fibroin protein film immersed with silver nitrate solution step (2) being obtained is exposed under sunlight, and illumination action time is 3h; With deionized water wash 5 times, room temperature is dried; Obtain nano thread fibroin-nanometer silver antimicrobial composite membrane.
3 one kinds of nano thread fibroin-nanometer silver antimicrobial matrix materials of embodiment.
Preparation nanometer fibroin protein film, and utilize nano thread fibroin film in-situ reducing Silver Nitrate to prepare nano thread fibroin-nanometer silver antimicrobial composite membrane, concrete steps are as follows:
(1) the nanometer silk fibroin water solution that preparation quality concentration is 3%, dropwise adds sodium hydrate regulator solution pH to 8 under stirring, then prepare nanometer fibroin protein film;
(2) nanometer fibroin protein film step (1) being obtained is immersed in the silver nitrate solution that concentration is 8 mg/mL, and soak time is 2.5h;
(3) the nanometer fibroin protein film immersed with silver nitrate solution step (2) being obtained is exposed under sunlight, and illumination action time is 5h; With deionized water wash 5 times, room temperature is dried; Obtain nano thread fibroin-nanometer silver antimicrobial composite membrane.
Claims (3)
1. a preparation method for nano thread fibroin-nanometer silver antimicrobial composite membrane, is characterized in that:
Preparation nanometer fibroin protein film, and utilize nano thread fibroin film in-situ reducing Silver Nitrate to prepare nano thread fibroin-nanometer silver antimicrobial composite membrane, concrete steps are as follows:
(1) the nanometer silk fibroin water solution that preparation quality concentration is 2-5%, dropwise adds sodium hydrate regulator solution pH to 8 under stirring, then prepare nanometer fibroin protein film;
(2) nanometer fibroin protein film step (1) being obtained is immersed in the silver nitrate solution that concentration is 5 ~ 10 mg/mL, and soak time is 1 – 2.5h;
(3) the nanometer fibroin protein film immersed with silver nitrate solution step (2) being obtained is exposed under sunlight, and illumination action time is 1-5h; With deionized water wash 5 times, room temperature is dried; Obtain nano thread fibroin-nanometer silver antimicrobial composite membrane.
2. nano thread fibroin-nanometer silver antimicrobial matrix material that the preparation method as described in claim 1 obtains.
3. the application of the fibroin-nanometer silver antimicrobial of the nano thread as described in claim 2 matrix material in food product pack, sanitary product, medical material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310554581.9A CN103665894A (en) | 2013-11-11 | 2013-11-11 | Nanometer silk fibroin-nano-silver antibacterial composite material |
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| CN201310554581.9A CN103665894A (en) | 2013-11-11 | 2013-11-11 | Nanometer silk fibroin-nano-silver antibacterial composite material |
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| CN103665894A true CN103665894A (en) | 2014-03-26 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105040416A (en) * | 2015-08-22 | 2015-11-11 | 苏州正业昌智能科技有限公司 | Nano-silver antibacterial blended fabric |
| CN105088780A (en) * | 2015-08-22 | 2015-11-25 | 苏州正业昌智能科技有限公司 | Nano-silver antibacterial cotton fabric |
| CN105854066A (en) * | 2016-04-14 | 2016-08-17 | 西南大学 | Method of modifying polyelectrolyte thin film coated sericin gel with nano-silver and product and application thereof |
| CN105903057A (en) * | 2016-04-14 | 2016-08-31 | 西南大学 | Preparation method of nano-silver hybrid sericin porous gel antibacterial material, product and application thereof |
| CN106268362A (en) * | 2015-06-12 | 2017-01-04 | 中国石油化工股份有限公司 | The preparation method of a kind of antimicrobial compound film and the antimicrobial compound film prepared by the method thereof and its application in water treatment field |
| CN106310351A (en) * | 2016-08-19 | 2017-01-11 | 蓝广芊 | Preparation method of nano-silver particle silkworm cocoon shell wound dressing |
| CN106674982A (en) * | 2016-12-12 | 2017-05-17 | 成都育芽科技有限公司 | Polyurethane antibacterial biomedical composite material and preparation method thereof |
| CN107400163A (en) * | 2017-07-17 | 2017-11-28 | 江南大学 | A kind of method by illumination synthetic proteins silver nanoparticle heterodimer |
| CN107641965A (en) * | 2016-07-22 | 2018-01-30 | 盐城工业职业技术学院 | A kind of preparation technology of antibiotic antistatic finishing agent and its manufactured coral fleece blanket fabrics |
| CN113647409A (en) * | 2021-08-11 | 2021-11-16 | 武汉纺织大学 | Silver-loaded silk fibroin nanoparticle antibacterial agent, preparation method and application |
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| JPH11228837A (en) * | 1998-02-16 | 1999-08-24 | National Institute Of Sericultual And Entomological Science | Silk protein/collagen composite material and its production |
| CN102618043A (en) * | 2012-04-15 | 2012-08-01 | 复旦大学 | Fibroin-silver-nanoparticle composite material and preparation method thereof |
| CN102816440A (en) * | 2012-09-10 | 2012-12-12 | 复旦大学 | Isolated soy protein-nano silver antibacterial composite membrane and preparation method thereof |
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2013
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Patent Citations (3)
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| JPH11228837A (en) * | 1998-02-16 | 1999-08-24 | National Institute Of Sericultual And Entomological Science | Silk protein/collagen composite material and its production |
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| CN102816440A (en) * | 2012-09-10 | 2012-12-12 | 复旦大学 | Isolated soy protein-nano silver antibacterial composite membrane and preparation method thereof |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106268362A (en) * | 2015-06-12 | 2017-01-04 | 中国石油化工股份有限公司 | The preparation method of a kind of antimicrobial compound film and the antimicrobial compound film prepared by the method thereof and its application in water treatment field |
| CN106268362B (en) * | 2015-06-12 | 2018-12-28 | 中国石油化工股份有限公司 | A kind of preparation method of antimicrobial compound film and its antimicrobial compound film and its application in water treatment field by this method preparation |
| CN105088780A (en) * | 2015-08-22 | 2015-11-25 | 苏州正业昌智能科技有限公司 | Nano-silver antibacterial cotton fabric |
| CN105040416A (en) * | 2015-08-22 | 2015-11-11 | 苏州正业昌智能科技有限公司 | Nano-silver antibacterial blended fabric |
| CN105903057A (en) * | 2016-04-14 | 2016-08-31 | 西南大学 | Preparation method of nano-silver hybrid sericin porous gel antibacterial material, product and application thereof |
| CN105854066A (en) * | 2016-04-14 | 2016-08-17 | 西南大学 | Method of modifying polyelectrolyte thin film coated sericin gel with nano-silver and product and application thereof |
| CN105854066B (en) * | 2016-04-14 | 2019-01-22 | 西南大学 | Method of nanometer modified by silver polyelectrolyte membrane coating sericin gel and products thereof and application |
| CN105903057B (en) * | 2016-04-14 | 2019-01-22 | 西南大学 | Preparation method of nano silver hydridization silk gum porous gel anti-biotic material and products thereof and application |
| CN107641965A (en) * | 2016-07-22 | 2018-01-30 | 盐城工业职业技术学院 | A kind of preparation technology of antibiotic antistatic finishing agent and its manufactured coral fleece blanket fabrics |
| CN106310351A (en) * | 2016-08-19 | 2017-01-11 | 蓝广芊 | Preparation method of nano-silver particle silkworm cocoon shell wound dressing |
| CN106674982A (en) * | 2016-12-12 | 2017-05-17 | 成都育芽科技有限公司 | Polyurethane antibacterial biomedical composite material and preparation method thereof |
| CN107400163A (en) * | 2017-07-17 | 2017-11-28 | 江南大学 | A kind of method by illumination synthetic proteins silver nanoparticle heterodimer |
| CN107400163B (en) * | 2017-07-17 | 2020-10-23 | 江南大学 | Method for synthesizing protein-silver nano heterodimer by illumination |
| CN113647409A (en) * | 2021-08-11 | 2021-11-16 | 武汉纺织大学 | Silver-loaded silk fibroin nanoparticle antibacterial agent, preparation method and application |
| CN113647409B (en) * | 2021-08-11 | 2023-03-07 | 武汉纺织大学 | Silver-loaded silk fibroin nanoparticle antibacterial agent, preparation method and application |
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Application publication date: 20140326 |
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