CN103656711A - Deodorant solution and preparation method and preparation kit thereof - Google Patents
Deodorant solution and preparation method and preparation kit thereof Download PDFInfo
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- CN103656711A CN103656711A CN201210375846.4A CN201210375846A CN103656711A CN 103656711 A CN103656711 A CN 103656711A CN 201210375846 A CN201210375846 A CN 201210375846A CN 103656711 A CN103656711 A CN 103656711A
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- bacillus subtilis
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Abstract
The invention provides a deodorant which is safe to human body, avoids degradation of metals and the like and is cheap and high in continuity. The invention provides a deodorant solution in which the cell concentration of bacillus subtilis is higher than 7.6/ml and lower than 3.8*10<5>/ml. The bacillus subtilis easy to reproduce is adopted in the deodorant solution, and thus mass production can be realized at low price. Moreover, the bacillus subtilis has spore forming property and excellent heat resistance, and thus the deodorant solution and a stock solution for dilution are easy to store. Moreover, the deodorant solution provided by the invention contains bacillus subtilis of which the cell concentration is higher than 7.6/ml and lower than 3.8*10<5>/ml, can realize quick reproduction of bacillus subtilis and thus can expel the fungi leading to deodorization and effectively eliminate odor.
Description
Technical field
The present invention relates to a kind of deodorant liquid, its preparation method of the deodorization for the smelly grade of rotting and prepare test kit.
Background technology
At present, as the method for removing the stinks such as rubbish or dung urine, known have by utilize the chemical reaction of oxidation that ozone causes etc. stink molecule is become odorless composition method or use active carbon to make the method for stink Molecular Adsorption.In addition, also known have by making to become the stink molecule of root of stench and the method that cyclodextrin equimolecular Composite carrys out deodorization.
Summary of the invention
Invent problem to be solved
While using oxidant as ozone etc., if to metallic article use, the metallic article that likely causing gets rusty causes deteriorated.In addition, the large multipair human body of the composition stench of the smelly grade of corruption Organic being decomposed is harmful to.Active carbon high price, but there is the problem that must exchange continually.Method by absorption stink molecules such as cyclodextrin also has the high and effect of easy cost and is difficult to lasting difficult point.
In the time of from the viewpoint, need to be to human-body safety, do not make that metal etc. is deteriorated, cheapness and the high deodorizer of persistence.
For solving the method for problem
For this invention that solves above-mentioned problem, relate to a kind of deodorant liquid, it contains bacillus subtilis and is greater than 7.6/ml, preferably 7.6 * 10/more than ml, with upper limit concentration lower than 3.8 * 10
5individual/ml, preferably 1.9 * 10
5individual/cell concentration below ml contains.
In above-mentioned deodorant liquid, above-mentioned bacillus subtilis is for being selected from bacillus acidocaldarius (Bacillus acidocaldarius), bacillus alvei (Bacillus alvei), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), bacillus brevis (Bacillus brevis), filmanetous colony bacillus (Bacillus filicolonicus), Bacillus megatherium (Bacillus megaterium), bacillus pumilus (Bacillus pumilus), Bacillus sphaericus (Bacillus sphaericus), at least one bacillus subtilis in bacillus subtilis (Bacillus subtilis) and bacillus subtilis hay subspecies (Bacillus subtilis subsp subtilis).
In addition, in above-mentioned deodorant liquid, above-mentioned bacillus subtilis is in activated state.
And this invention also relates to the preparation method of above-mentioned deodorant liquid.
In said method, above-mentioned deodorant liquid is prepared by following operation, comprising: dilution operation, take above-mentioned deodorant liquid as 4.0 * 10
5individual/m1 is above, preferably 4.0 * 10
6individual/m1 is above, more preferably 4.0 * 10
7individual/ml is above, further preferably 4.0 * 10
8individual/ml is above and be 2.0 * 10
9individual/m1 is following, preferably 1.0 * 10
9the stock solution of the above-mentioned bacillus subtilis that individual/cell concentration below ml contains inactive state is diluted, and dilutes for higher than 7.6/ml, preferably above the and upper limit concentration of 7.6 * 10/ml is lower than 3.8 * 10
5individual/ml, preferably 1.9 * 10
5individual/cell concentration below m1; And activation procedure, by the above-mentioned stock solution that dilution is obtained, at 40 ℃～60 ℃, heat, make above-mentioned bacillus subtilis activation.
And this invention relates to deodorant liquid and prepares test kit, wherein, contain the unit of above-mentioned preparation method being informed to the user of above-mentioned deodorant liquid with above-mentioned stock solution simultaneously.
The effect of invention
Bacillus subtilis because the deodorant liquid of this invention is used easy propagation, therefore, can produce at an easy rate in a large number.In addition, bacillus subtilis has the character that forms brood cell, excellent heat resistance, and therefore, the stock solution of this deodorant liquid and dilution use thereof is easily taken care of.And the deodorant liquid of this invention, by with higher than 7.6/ml, lower than 3.8 * 10
5the cell concentration of individual/ml contains bacillus subtilis, can carry out the rapid propagation of bacillus subtilis, therefore, can suppress to become the effect of mushroom of the reason of deodorization, can remove efficiently stench.
In a mode of the deodorant liquid of this invention, bacillus subtilis is for being selected from bacillus acidocaldarius (Bacillus acidocaldarius), bacillus alvei (Bacillus alvei), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), bacillus brevis (Bacillus brevis), filmanetous colony bacillus (Bacillus filicolonicus), Bacillus megatherium (Bacillus megaterium), bacillus pumilus (Bacillus pumilus), Bacillus sphaericus (Bacillus sphaericus), at least one bacillus subtilis in bacillus subtilis (Bacillus subtilis) and bacillus subtilis hay subspecies (Bacillus subtilis subsp subtilis), therefore, harmless, aspect environment, can not bring the baneful influence in health.
In addition, in the deodorant liquid due to this invention, contained bacillus subtilis is activated state, and therefore, while scattering, propagation, promptly manifests deodorizing effect at once.
In the preparation method of the deodorant liquid of this invention, from 4.0 * 10
5individual/ml is above, preferably 4.0 * 10
6individual/m1 is above, more preferably 1.0 * 10
7individual/ml is above, further preferably 4.0 * 10
8above and the 2.0 * l0 of individual/ml
9individual/m1 is following, preferably 1.0 * 10
9the stock solution of the bacillus subtilis that individual/cell concentration below ml contains inactive state is prepared deodorant liquid.This stock solution is except containing thalline with high concentration, and thalline is inactive state, thus, compares with deodorant liquid, and storage stability is higher, therefore, replacing that deodorant liquid is preserved, aspect conveying, is favourable.
At the deodorant liquid of this invention, prepare in the test kit of use, contain the stock solution of the preparation method of deodorant liquid being informed to unit and the deodorant liquid of user, therefore, user can easily be prepared deodorant liquid.
Accompanying drawing explanation
Fig. 1 represents the result of the proliferation experiment of the bacillus subtilis in each diluted concentration.
Fig. 2 represents the result of the proliferation experiment of the bacillus subtilis in each diluted concentration.
The specific embodiment
The described deodorant liquid of this invention contains higher than 7.6/ml, lower than 3.8 * 10
5the bacillus subtilis of the cell concentration of individual/ml.Generally speaking, mushroom is when propagation, and the thalline number before propagation is larger, thalline number after propagation is also larger, but the character that bacillus subtilis does not breed while having when propagation starts in high density state, therefore, in this invention, determine the concentration of the propagation that is suitable for bacillus subtilis most.In this description, represent that the numeral of the thalline number of bacillus subtilis is all used 2 records of significant digits.Here, bacillus subtilis is preferably selected from bacillus acidocaldarius (Bacillus acidocaldarius), bacillus alvei (Bacillus alvei), bacillus amyloliquefaciens (Bacillus amyloliquefaciens), bacillus brevis (Bacillus brevis), filmanetous colony bacillus (Bacillus filicolonicus), Bacillus megatherium (Bacillus megaterium), bacillus pumilus (Bacillus pumilus), Bacillus sphaericus (Bacillus sphaericus), bacillus subtilis (Bacillus subtilis) and bacillus subtilis hay subspecies (Bacillus subtilis subsp subtilis).Bacillus subtilis can contain above-mentioned a kind, also can contain two or more.Preferably bacillus subtilis contains above-mentioned 1～5 kind, most preferably contains 5 kinds.
In the use form of preferred deodorant liquid, bacillus subtilis is in activated state.In this manual, " activated state " of bacillus subtilis refers to the state germinateing from brood cell, and " inactive state " refers to the resting state that is formed with brood cell.
In the preparation method of the described deodorant liquid of this invention, will be with 4.0 * 10
5individual/ml is above, preferably 4.0 * 10
6individual/ml is above, more preferably 4.0 * 10
7individual/m1 is above, further preferred 4.0 * l0
8individual/ml is above and 2.0 * 10
9individual/m1 is following, preferably 1.0 * 10
9the stock solution of the bacillus subtilis that individual/cell concentration below m1 contains inactive state is diluted to higher than 7.6/ml, lower than 3.8 * 10
5the cell concentration of individual/ml, heats and makes its activation, obtains thus deodorant liquid.This stock solution is stable when keeping.This stock solution can utilize acetate buffer solution or water etc. to dilute.Generally speaking, with the dilution of tap water, be easy.When the bacillus subtilis activation that makes inactive state, also can use the germination inductive substances such as aminoacid or sugar.
The activation condition of bacillus subtilis in inactive state is the heating of 20 minutes～60 minutes at 40 ℃～60 ℃, is preferably the heating at 45 ℃～50 ℃.
By under these conditions the bacillus subtilis of inactive state being heated, bacillus subtilis becomes the activated state of germinateing, can breed under nutritional condition from brood cell.Here, under nutritional condition, refer to the state culture medium such as SCDM, house refuse or dung urine etc., under the existence of material of bacillus subtilis in absorbing nutrition.
In the test kit of this invention, for the preparation of the stock solution of deodorant liquid with preparation method is informed to the unit of user provides simultaneously.
As the unit of informing user, for example, can enumerate utilize word or illustration to record to hold the operation instructions of intelligible preparation method, the accepting container of stock solution, for the container that uses when the dilution stock solution, for being attached at the label etc. of these containers.Said units can be for recording sound or image or its both storage medium that using method is described.
[embodiment 1]
(in bacillus subtilis liquid A, the cell concentration of contained bacillus subtilis is measured)
Measure the cell concentration of the bacillus subtilis in bacillus subtilis liquid A.This bacillus subtilis liquid A is from natural bacillus subtilis, at least contains bacillus subtilis, does not contain bacillus subtilis antibacterial and fungus in addition.
(assay method)
The following cell concentration of measuring bacillus subtilis contained in bacillus subtilis liquid A.
(1) under aseptic condition, by bacillus subtilis liquid A, with aquesterilisa dilution, be 4,000 times, further repeat to carry out for 3 times 10 times of dilutions of the 0.1%Tween80 solution of autoclaving, 4 * 10 of preparation bacillus subtilis liquid A
4doubly, 4 * 10
5doubly with 4 * 10
6ladder dilution series doubly.
(2) each ladder diluent 0.5m1 is moved to 3 sterilized culture dishs, by carried out autoclaving and in 50 ℃ insulation Semen sojae atricolor casein digesting agar culture medium (with the pure medicine of light) 20ml dispensing in culture dish, after stirring lightly, culture medium is solidified.
(3) in 30～35 ℃, cultivate 24 hours counting clump count.
(result)
Bacterium is counted measurement result, with regard to 4 * 10
6diluent doubly, every 1 culture dish is 94,84 and 104.That is,, while averaging, every 1 culture dish is 94.
Therefore, dilution is that the cell concentration of bacillus subtilis contained in the bacillus subtilis liquid A of 4,000 times is 1.9 * 10
5individual/m1, the cell concentration of the bacillus subtilis liquid A of stock solution is 7.6 * 10
8individual/ml.
[embodiment 2]
(proliferation experiment in each dilution ratio of bacillus subtilis liquid A)
(method)
(1) to the bacillus subtilis liquid A using in embodiment 1,10 times of dilutions of the SCDM that repeats to cross with autoclaving under aseptic condition (with the pure medicine of light), preparation 10～10
7ladder dilution series doubly (experiment 1).Similarly operation, prepares 10 times, 10
2doubly, 10
3doubly, 2 * 10
3doubly, 4 * 10
3doubly, 6 * 10
3doubly, 8 * 10
3doubly with 10
4ladder dilution series doubly (experiment 2).
(2) each dilution series is heated in 40 ℃ after 1 hour, in 35 ℃, cultivate 36 hours.After cultivation, by visualization, judge the muddiness of the culture medium that the propagation of antibacterial causes.
(result)
In Fig. 1, represent the result of experiment 1.In Fig. 1, the project of bacterial multiplication is recited as to the situation of "+", represent to see and breed the muddiness causing, be recited as the situation of "-", represent the muddiness of not seeing that propagation causes.As can be seen from Figure 1, diluting 10
4doubly in above sample, all see the muddiness of culture medium.
In addition, the result that represents experiment 2 in Fig. 2.In Fig. 2, the project of bacterial multiplication is recited as to the situation of "+", represent to see and breed the muddiness causing, be recited as the situation of "-", represent the muddiness of not seeing that propagation causes.As can be seen from Figure 2,2 * 10
3in dilution doubly, do not see the muddiness of culture medium, 4 * 10
3doubly, in above dilution, see the muddiness of culture medium, thus, be presented at and surpass 2 * 10
3in dilution doubly, see muddiness.
Therefore, known by bacillus subtilis liquid A is surpassed to 2 * 10
3doubly dilute, preferably dilution is 4 * 10
3doubly, form the concentration of the propagation that is suitable for bacillus subtilis.In addition, in the scope of testing, even if be diluted to 10
7times time, also fully confirm muddy, therefore, known so long as lower than 10
8dilution doubly, produces muddy.That is the concentration of propagation that, is suitable for bacillus subtilis is for lower than 3.8 * 10
5individual/ml, preferably 1.9 * 10
5individual/cell concentration below ml.The least concentration of bacillus subtilis in deodorant liquid is the cell concentration higher than 7.6/ml, preferably 7.6 * 10/more than ml.
[embodiment 3]
(sensory test of the deodorizing effect of deodorant liquid)
(method)
(cultivation of Anaerobic Bacteria)
(1) in sterilized culture dish, on curing standard agar culture medium (day water pharmacy), smear from natural freeze-dried bias Anaerobic Bacteria clostridium sporogenes (Clostridium sporogenes), in the thermostat of 30 ℃, cultivate after 48～72 hours, confirm the formation of bacterium colony.
(2) in the sterilized vial of 250ml, put into SCDM (with the pure medicine of light), 1 of the bacterium colony that forms has been confirmed in inoculation therein in order (1), by rubber bolt, covers tightly.In the thermostat of 30 ℃, cultivate 3 days.
(3) after cultivating, confirm the pH of culture medium, result is pH5.95.Used sterilized Soren gloomy (Sorensen) phosphate buffer (pH7) to be adjusted into pH6.10.Its dispensing, in 2 (A, B) each 100m1 of conical flask for sterilized shaken cultivation of 500ml, is covered to silicon plug (registered trade mark).
(interpolation of bacillus subtilis liquid A)
(5) by 45 ℃ heated 30 minutes with aquesterilisa, diluted 4.0 * l0
3bacillus subtilis liquid A10m1 doubly, as the sample of deodorant liquid, makes an addition in conical flask A.Sample adds aquesterilisa 10m1 in conical flask B in contrast.
(6) by shaken cultivation machine for conical flask A, B (B.BRAUN CERTOMAT(registered trade mark processed) U) in 30 ℃, carry out shaken cultivation.
(7) shaken cultivation of conical flask A, B started after 24 hours, took off silicon plug (registered trade mark), further in conical flask A, B, added respectively sample and control sample 10ml separately.Recycle silicon plug (registered trade mark) covers tightly, and further carries out shaken cultivation.
(8) shaken cultivation of conical flask A, B started after 48 hours, took off silicon plug (registered trade mark), further in conical flask A, B, added respectively sample and control sample 10ml separately.Here, by stink, identified the judgement of the stink in Shi Jinhang flask.Recycle silicon plug (registered trade mark) covers tightly, and further carries out shaken cultivation.
(9) shaken cultivation at conical flask A, B started after 72 hours, took off silicon plug (registered trade mark), further in conical flask A, B, added respectively sample and control sample 10ml separately.Recycle silicon plug (registered trade mark) covers tightly, and further carries out shaken cultivation.
(10) shaken cultivation at conical flask A, B started after 96 hours, took off silicon plug (registered trade mark), further in conical flask A, B, added respectively sample and control sample 10ml separately.Here, by 1 stink, identified the judgement of the stink in Shi Jinhang flask.
(result)
With 10 grades, evaluate the judgement that represents stink.The most weak stink is set as to 1, the strongest stink is set as to 10.
Result is as described in following table 1.
| Flask A(sample) | Flask B (control sample) | |
| After 48 |
5 | 10 |
| After 96 |
3 | 9 |
After 48 hours, from adding the main stink that has the flask of control sample B to send, being scatol, after 96 hours, is ammonia from adding the main stink that has the flask of control sample B to send.
As known from the above, 4.0 * 10 of bacillus subtilis liquid A
3times diluent is (with 1.9 * 10
5the cell concentration of individual/ml contains bacillus subtilis) there is deodorizing effect.
Claims (9)
1. a deodorant liquid, is characterized in that:
Contain bacillus subtilis,
The cell concentration of described bacillus subtilis is higher than 7.6/m1, lower than 3.8 * 10
5individual/ml.
2. deodorant liquid as claimed in claim 1, is characterized in that:
The cell concentration of described bacillus subtilis be 7.6 * 10/ml above, 1.9 * 10
5individual/below ml.
3. deodorant liquid as claimed in claim 1 or 2, is characterized in that:
Described bacillus subtilis is at least one bacillus subtilis being selected from bacillus acidocaldarius, bacillus alvei, bacillus amyloliquefaciens, bacillus brevis, filmanetous colony bacillus, Bacillus megatherium, bacillus pumilus, Bacillus sphaericus, bacillus subtilis and bacillus subtilis hay subspecies.
4. deodorant liquid as claimed in claim 1 or 2, is characterized in that:
Described bacillus subtilis is in activated state.
5. a method of preparing deodorant liquid, is characterized in that:
Use is with 4.0 * 10
5individual/ml is above, 2.0 * 10
9the stock solution that individual/cell concentration below ml contains the bacillus subtilis in inactive state, prepares cell concentration higher than 7.6/ml, lower than 3.8 * 10
5the deodorant liquid of individual/ml.
6. method as claimed in claim 5, is characterized in that, comprises following operation:
Dilution operation, dilutes described stock solution, dilutes for higher than 7.6/ml, lower than 3.8 * 10
5the cell concentration of individual/m1; With
Activation procedure, heats at 40 ℃～60 ℃ by the described stock solution that dilution is obtained, and makes described bacillus subtilis activation.
7. method as claimed in claim 5, is characterized in that:
Described bacillus subtilis is at least one bacillus subtilis being selected from bacillus acidocaldarius, bacillus alvei, bacillus amyloliquefaciens, bacillus brevis, filmanetous colony bacillus, Bacillus megatherium, bacillus pumilus, Bacillus sphaericus, bacillus subtilis and bacillus subtilis hay subspecies.
8. method as claimed in claim 5, is characterized in that:
Bacillus subtilis contained in described deodorant liquid is in activated state.
9. a deodorant liquid preparation test kit, is characterized in that:
Comprise unit and the stock solution claimed in claim 5 of method claimed in claim 5 being informed to user.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210375846.4A CN103656711A (en) | 2012-09-25 | 2012-09-25 | Deodorant solution and preparation method and preparation kit thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210375846.4A CN103656711A (en) | 2012-09-25 | 2012-09-25 | Deodorant solution and preparation method and preparation kit thereof |
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| Publication Number | Publication Date |
|---|---|
| CN103656711A true CN103656711A (en) | 2014-03-26 |
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|---|---|---|---|
| CN201210375846.4A Pending CN103656711A (en) | 2012-09-25 | 2012-09-25 | Deodorant solution and preparation method and preparation kit thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105617430A (en) * | 2016-01-15 | 2016-06-01 | 中林山水（北京）生态科技股份有限公司 | Organic waste deodorant and preparation method thereof |
| CN105816897A (en) * | 2016-03-17 | 2016-08-03 | 中创宏远（北京）环保科技有限公司 | Organic garbage deodorant containing microbial strains and preparation method thereof |
| CN105944130A (en) * | 2016-05-24 | 2016-09-21 | 山东省科学院生物研究所 | Odor-resistant and antibacterial pad attached with probiotics and production method and application of odor-resistant and antibacterial pad |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85106098A (en) * | 1985-07-15 | 1987-01-14 | 财团法人成研会 | Biological deodorant and preparation method thereof |
| CN1434005A (en) * | 2003-03-05 | 2003-08-06 | 湖南大学 | Deodorant, deodorizing accelerator and deodorizing method for refuse compost |
| US20060246040A1 (en) * | 2004-01-09 | 2006-11-02 | Ajinomoto Co., Inc. | Deodorizing agent containing dry cell of microorganism as active ingredient and deodorizing method |
| CN101292022A (en) * | 2005-10-22 | 2008-10-22 | 朴世俊 | Microorganisms having bad smell removal activity of organic waste and use thereof |
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
-
2012
- 2012-09-25 CN CN201210375846.4A patent/CN103656711A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85106098A (en) * | 1985-07-15 | 1987-01-14 | 财团法人成研会 | Biological deodorant and preparation method thereof |
| CN1434005A (en) * | 2003-03-05 | 2003-08-06 | 湖南大学 | Deodorant, deodorizing accelerator and deodorizing method for refuse compost |
| US20060246040A1 (en) * | 2004-01-09 | 2006-11-02 | Ajinomoto Co., Inc. | Deodorizing agent containing dry cell of microorganism as active ingredient and deodorizing method |
| CN101292022A (en) * | 2005-10-22 | 2008-10-22 | 朴世俊 | Microorganisms having bad smell removal activity of organic waste and use thereof |
| CN101638626A (en) * | 2009-09-10 | 2010-02-03 | 惠飞 | Bacillus subtilis and application thereof in biological deodorant |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105617430A (en) * | 2016-01-15 | 2016-06-01 | 中林山水（北京）生态科技股份有限公司 | Organic waste deodorant and preparation method thereof |
| CN105816897A (en) * | 2016-03-17 | 2016-08-03 | 中创宏远（北京）环保科技有限公司 | Organic garbage deodorant containing microbial strains and preparation method thereof |
| CN105816897B (en) * | 2016-03-17 | 2018-10-16 | 中创宏远（北京）环保科技有限公司 | A kind of organic waste deodorant and preparation method thereof containing microbial strains |
| CN105944130A (en) * | 2016-05-24 | 2016-09-21 | 山东省科学院生物研究所 | Odor-resistant and antibacterial pad attached with probiotics and production method and application of odor-resistant and antibacterial pad |
| CN105944130B (en) * | 2016-05-24 | 2018-08-24 | 山东省科学院生物研究所 | A kind of deodorization antibacterial pad and the preparation method and application thereof of absorption profitable probliotics |
| CN112779182A (en) * | 2020-12-31 | 2021-05-11 | 武汉工程大学 | Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base |
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Application publication date: 20140326 |