CN103655821A - Traditional Chinese medicine preparation with effects of benefiting Qi and raising Yang as well as replenishing essence and tonifying marrow and preparation method of traditional Chinese medicine preparation - Google Patents
Traditional Chinese medicine preparation with effects of benefiting Qi and raising Yang as well as replenishing essence and tonifying marrow and preparation method of traditional Chinese medicine preparation Download PDFInfo
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Abstract
The invention relates to the field of traditional Chinese medicines and particularly relates to a traditional Chinese medicine preparation with effects of benefiting Qi and raising Yang as well as replenishing essence and tonifying marrow and a preparation method of the traditional Chinese medicine preparation. The adopted technical scheme is as follows: the traditional Chinese medicine preparation is prepared from the following raw materials in parts by weight: 90-140 parts of ginseng, 40-80 parts of morinda officinalis, 15-25 parts of pilose antler, 50-70 parts of cistanche deserticola, 50-70 parts of seahorse, 50-70 parts of eucommia ulmoides, 50-70 parts of spirifer, 100-140 parts of epimedium herbs, 15-25 parts of clove, 15-25 parts of garden balsam, 15-25 parts of gecko, 100-130 parts of dogwood, 100-135 parts of fructus cnidii, 100-135 parts of oysters, 900-1050 parts of astragalus, 40-60 parts of earthworms, 40-60 parts of cinnamomum cassia and 40-65 parts of schisandra chinensis. According to the technical scheme, the traditional Chinese medicine preparation has the beneficial effects of benefiting Qi and raising Yang as well as replenishing essence and tonifying marrow, and can be used for treating diseases such as dizziness, shortness of breath and hypodynamia, spermatorrhea and the like caused by insufficiency of kidney-Yang and yin fluid loss. Clinical tests prove that the traditional Chinese medicine preparation has an excellent treatment effect.
Description
Technical field
The present invention relates to the field of Chinese medicines, relate in particular to Chinese medicine preparation of a kind of QI invigorating tonifying YANG and benefiting essence and marrow and preparation method thereof.
Background technology
Along with modern's life pressure increases, increasing people is in sub-health state, and the medicinal liquid of current various QI invigorating body-building is more and more, however various therapeutic effect differ, respectively have quality.
Western medicine is due to highly purified molecule cluster, and concentrated absorption is unavoidably in evil case, to the heart, brain, liver, the equal toxic side effect of kidney.
Summary of the invention
The object of invention: for Chinese medicine preparation of a kind of QI invigorating tonifying YANG and benefiting essence and marrow and preparation method thereof is provided.
In order to reach as above object, the present invention takes following technical scheme: its raw material by following components by weight percent is prepared from: Radix Ginseng 90-140 part; Radix Morindae Officinalis 40-80 part; Cornu Cervi Pantotrichum 15-25 part; Herba Cistanches 50-70 part; Hippocampus 50-70 part; Cortex Eucommiae 50-70 part; Fossilia Spiriferis 50-70 part; Herba Epimedii 100-140 part; Flos Caryophylli 15-25 part; Semen Impatientis 15-25 part; Gecko 15-25 part; Fructus Corni 100-130 part; Fructus Cnidii 100-135 part; Concha Ostreae 100-135 part; Radix Astragali 900-1050 part; Pheretima 40-60 part; Cortex Cinnamomi 40-60 part; Fructus Schisandrae Chinensis 40-65 part.
The further technical scheme of the present invention is, its raw material by following components by weight percent is prepared from: 120 parts of Radix Ginsengs; 60 parts of Radix Morindae Officinaliss; 20 parts, Cornu Cervi Pantotrichum; 60 parts of Herba Cistanches; 60 parts of Hippocampus; 60 parts of the Cortexs Eucommiae; 60 parts, Fossilia Spiriferis; 120 parts of Herba Epimedii; 20 parts of Flos Caryophyllis; 20 parts of Semen Impatientis; 20 parts of Geckos; 120 parts of Fructus Corni; 120 parts of Fructus Cnidiis; 120 parts of Concha Ostreaes; 1000 parts of the Radixs Astragali; 60 parts of Pheretimas; 60 parts of Cortex Cinnamomis; 60 parts of Fructus Schisandrae Chinensis.
The method of preparation preparation as above, is characterized in that: comprise following steps:
● just make: Radix Ginseng, Cortex Cinnamomi, Flos Caryophylli, the Cortex Eucommiae are dried, and are ground into powder, cross 60 mesh sieves, mix with Fructus Cnidii;
● extraction and concentration: add 80% alcohol reflux twice, add for the first time alcohol 4 volumes and doubly measure, extract 8 hours, add for the second time alcohol 2 volumes and doubly measure, extract merge extractive liquid, 6 hours, filter, decompression filtrate recycling ethanol, and be concentrated into the extractum of relative density 1.30～1.34 (80 ℃);
● decoct and concentrate: medicinal residues and Herba Cistanches, Radix Morindae Officinalis, Concha Ostreae, Fossilia Spiriferis, Fructus Schisandrae Chinensis, Semen Impatientis, Herba Epimedii, Fructus Corni, the Radix Astragali decoct with water three times, adding for the first time water 10 volumes doubly measures, decoct 2 hours, add for the second time water 8 volumes and doubly measure, decoct 2 hours, add for the third time water 6 volumes and doubly measure, decoct 1 hour, collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Cornu Cervi Pantotrichum, Hippocampus, Gecko, Pheretima are dried, pulverize, decoct with water twice, add for the first time water 8 volumes and doubly measure, add for the second time water 6 volumes and doubly measure, decoct respectively 2 hours, collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃);
● regulate PH and concentrated: medicinal residues add water, add hydrochloric acid and regulate pH value to 4.5～5.0, in 100 ℃ of ordinary-pressure hydrolysis 24 hours, filter, filtrate adds 10% sodium hydroxide solution and regulates pH value to 6.5～7.0, filters, and filtrate decompression is concentrated into the extractum of relative density 1.30～1.34 (80 ℃).
The further technical scheme of the present invention is, also comprises the step of granulation, merges extractum, mixes, and adds starch, mixes, and dry, pulverize into fine powder, with sucrose, adds ethanol granulation, is drying to obtain.
Adopt as above the present invention of technical scheme, there is following beneficial effect: QI invigorating tonifying YANG, benefiting essence and marrow, for insufficiency of kidney-YANG, having a dizzy spell due to deficiency of YIN-essence, shortness of breath and fatigue, the diseases such as seminal emission.Through clinical trial, find that there is fabulous therapeutic effect.
The specific embodiment
Below embodiments of the invention are described, embodiment is not construed as limiting the invention:
Be below embodiments of the invention 1, i.e. optimum embodiment:
120 parts of [prescription] Radix Ginsengs, 60 parts of Radix Morindae Officinaliss, 20 parts, Cornu Cervi Pantotrichum, 60 parts of Herba Cistanches, 60 parts of Hippocampus, 60 parts of the Cortexs Eucommiae, 60 parts, Fossilia Spiriferis, 120 parts of Herba Epimedii, 20 parts of Flos Caryophyllis, 20 parts of Semen Impatientis, 20 parts of Geckos, 120 parts of Fructus Corni, 120 parts of Fructus Cnidiis, 120 parts of Concha Ostreaes, 1000 parts of the Radixs Astragali, 60 parts of Pheretimas, 60 parts of Cortex Cinnamomis, 60 parts of Fructus Schisandrae Chinensis.
[method for making] above 18 tastes, Radix Ginseng, Cortex Cinnamomi, Flos Caryophylli, the Cortex Eucommiae are dried, and are ground into coarse powder, mix with Fructus Cnidii, add 80% alcohol reflux twice, add for the first time alcohol 4 volumes and doubly measure, extract 8 hours, adding for the second time alcohol 2 volumes doubly measures, extract 6 hours, merge extractive liquid,, filters, decompression filtrate recycling ethanol is to without alcohol taste, and is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Medicinal residues and Herba Cistanches, Radix Morindae Officinalis, Concha Ostreae, Fossilia Spiriferis, Fructus Schisandrae Chinensis, Semen Impatientis, Herba Epimedii, Fructus Corni, the Radix Astragali decoct with water three times, adding for the first time water 10 volumes doubly measures, decoct 2 hours, add for the second time water 8 volumes and doubly measure, decoct 2 hours, add for the third time water 6 volumes and doubly measure, decoct 1 hour, collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Four tastes such as all the other Cornu Cervi Pantotrichums, dry, pulverize, decoct with water twice, add for the first time water 8 volumes doubly to measure, and add for the second time water 6 volumes doubly to measure, and decoct respectively 2 hours, and collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Medicinal residues add water, add hydrochloric acid and regulate pH value to 4.5～5.0, in 100 ℃ of ordinary-pressure hydrolysis 24 hours, filter, and filtrate adds 10% sodium hydroxide solution and regulates pH value to 6.5～7.0, filters, and filtrate decompression is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Merge above-mentioned extractum, mix, add starch 250g, mix, dry, pulverize into fine powder, with sucrose 500g, add Diluted Alcohol granulation, dry, make 1000g, obtain.
Embodiment 2; Method for making is with embodiment 1; Its formula is as follows:
Its raw material by following components by weight percent is prepared from: 90 parts of Radix Ginsengs; 40 parts of Radix Morindae Officinaliss; 15 parts, Cornu Cervi Pantotrichum; 50 parts of Herba Cistanches; 50 parts of Hippocampus; 50 parts of the Cortexs Eucommiae; 50 parts, Fossilia Spiriferis; 100 parts of Herba Epimedii; 15 parts of Flos Caryophyllis; 15 parts of Semen Impatientis; 15 parts of Geckos; 100 parts of Fructus Corni; 100 parts of Fructus Cnidiis; 100 parts of Concha Ostreaes; 900 parts of the Radixs Astragali; 40 parts of Pheretimas; 40 parts of Cortex Cinnamomis; 40 parts of Fructus Schisandrae Chinensis.
Embodiment 3; Method for making is with embodiment 1; Its formula is as follows:
Its raw material by following components by weight percent is prepared from: Radix Ginseng 140 minutes; 80 parts of Radix Morindae Officinaliss; 25 parts, Cornu Cervi Pantotrichum; 70 parts of Herba Cistanches; 70 parts of Hippocampus; 70 parts of the Cortexs Eucommiae; 70 parts, Fossilia Spiriferis; 140 parts of Herba Epimedii; 25 parts of Flos Caryophyllis; 25 parts of Semen Impatientis; 25 parts of Geckos; 130 parts of Fructus Corni; 135 parts of Fructus Cnidiis; 135 parts of Concha Ostreaes; 1050 parts of the Radixs Astragali; 60 parts of Pheretimas; 60 parts of Cortex Cinnamomis; Fructus Schisandrae Chinensis 40-65 part.
[character] this product is tan granule; Feeble QI raw meat, taste is sweet rear hardship first.
[discriminating] 1. gets this product 10g, and porphyrize adds chloroform 40ml, reflux 1 hour, discards chloroform solution, and medicinal residues volatilize, add water 0.5ml mix thoroughly moistening after, add water saturated n-butyl alcohol 20ml, supersound process 30 minutes, draw supernatant, add the ammonia solution that 3 volumes are doubly measured, shake up, place layering, get upper solution evaporate to dryness, residue adds methanol 1ml to be made to dissolve, as need testing solution, separately get Radix Ginseng control medicinal material 1g, be made in the same way of control medicinal material solution.Get again ginsenoside Rb1, Re reference substance, add methanol and make every 1ml containing the mixed solution of 2mg, as according to product solution, according to thin layer chromatography, test, draw need testing solution and control medicinal material solution respectively 10 1, reference substance solution 51, put on same silica gel g thin-layer plate respectively, take chloroform-ethyl acetate-methanol-water (15: 40: 22: 10) 10 ℃ of following lower floor's solution of placing are developing solvent, launch, take out, dry, spray, with 10% ethanol solution of sulfuric acid, is heated to clear spot at 105 ℃.In test sample chromatograph, respectively with reference substance and the corresponding position of control medicinal material chromatograph on, the speckle of aobvious same color.
2. get this product 2g, porphyrize, adds ethanol 5ml, and supersound process 5 minutes is placed, and gets supernatant evaporate to dryness, and residue adds ethanol 1ml to be made to dissolve, as need testing solution.Separately get Fructus Cnidii control medicinal material 0.3g, be made in the same way of control medicinal material solution.Get again osthole reference substance, add ethanol and make every 1ml containing the solution of 1mg, in contrast product solution.According to thin layer chromatography test, draw need testing solution 10 1, control medicinal material solution 21, reference substance solution 11, put on same silica gel g thin-layer plate respectively, the benzene-ethyl acetate (30: 1) of take is developing solvent, launches, take out, dry, put under ultra-violet lamp (365nm) and inspect, in test sample chromatograph, respectively with reference substance chromatograph and the corresponding position of control medicinal material chromatograph on, the fluorescence speckle of aobvious same color.
3. get this product 12g, porphyrize, add chloroform-n-butyl alcohol (2: 1) mixed solution 75ml, put in water-bath reflux 30 minutes, let cool, filter, filtrate evaporate to dryness, residue adds 2% potassium hydroxide methanol solution 50ml to be made to dissolve, put in water-bath reflux 1 hour, solution evaporate to dryness, residue adds water 30ml to be made to dissolve, move in separatory funnel, with chloroform-n-butyl alcohol (2: 1) mixed solution 50ml jolting, extract, divide and get chloroform-n-butanol layer, with 1% potassium dihydrogen phosphate 50ml washing, discard 1% potassium dihydrogen phosphate layer, extracting solution evaporate to dryness, residue adds methanol 1ml to be made to dissolve, as need testing solution.Separately get Radix Astragali control medicinal material 3g, add methanol 20ml, reflux 1 hour, filter, filtrate is added on neutral alumina column (100-120 order, 5g, internal diameter 10-15mm), with 40% methanol 100ml eluting, collect eluent, evaporate to dryness, residue adds water 30ml to be made to dissolve, with water saturated n-butanol extraction 2 times, and each 20ml merging n-butyl alcohol liquid, wash with water 2 times, each 20ml, discards water liquid, n-butyl alcohol liquid evaporate to dryness, residue adds methanol 0.5ml to be made to dissolve, in contrast medical material solution.Separately get astragaloside reference substance, add methanol and make every 1ml containing the solution of 1mg, product solution, tests according to thin layer chromatography in contrast, draw above-mentioned three kinds of solution each 51, put on same silica gel g thin-layer plate respectively, take chloroform-ethyl acetate-methanol-water (15: 40: 22: 10) 10 ℃ of following lower floor's solution of placing are developing solvent, launch, take out, dry, spray, with 10% ethanol solution of sulfuric acid, is heated to speckle colour developing at 105 ℃ clear.In test sample chromatograph, with the corresponding position of reference substance chromatograph on, aobvious identical sepia speckle; Under ultra-violet lamp (365nm), aobvious identical orange-yellow fluorescence speckle.
[inspection]
1. granularity: can not and can, by granule and the powder summation of sieving for No. five, must not cross 15% by sieve.
2. moisture: must not cross 6.0%.
3. melting: should all dissolve, must not have the foreign bodies such as breeze, allow slight haze.
4. limit test of microbe:
[assay] is according to high effective liquid chromatography for measuring.
The suitable application test of chromatographic condition and system: be filler with octadecylsilane chemically bonded silica; 0.075mol/L phosphoric acid solution (regulating pH value to 4.5 with triethylamine)-acetonitrile (73:27) is mobile phase; Detection wavelength is 270nm..Number of theoretical plate calculates and should be not less than 4000 by icariin peak.
The preparation of reference substance solution: precision takes the icariin reference substance 10mg that is dried to constant weight at 105 ℃, puts in 100ml measuring bottle, with dissolve with methanol and be diluted to scale, shakes up.Precision measures 5ml, puts in 10ml measuring bottle, adds mobile phase to scale, shakes up.Obtain (in every 1ml, containing icariin 50g).
The preparation of need testing solution: this product under content uniformity item, porphyrize, gets 3g, accurately weighed, to put in 100ml measuring bottle, precision adds 70% ethanol 50ml, weighed weight, placement is spent the night, supersound process 20 minutes, let cool, more weighed weight, with 70% ethanol, supply the weight of less loss, shake up, filter, get subsequent filtrate, obtain.
Algoscopy: respectively accurate draw reference substance solution and need testing solution each 101, injection liquid chromatography, mensuration, obtains.
Every bag of this product is containing icariin (C
33h
40o
15) must not be less than 2.0mg.
The tonifying YANG of [function cures mainly] QI invigorating, benefiting essence and marrow, for insufficiency of kidney-YANG, having a dizzy spell due to deficiency of YIN-essence, shortness of breath and fatigue, the diseases such as seminal emission.
[usage and dosage] boiled water is taken after mixing it with water, a 4g, and 2-3 time on the one, surrounding is a course for the treatment of.
[points for attention] (1) prostate hyperplasia person be cautious use of.
(2) hyperthyroidism, hyperpietic follow the doctor's advice.
[storage] seals, puts shady and cool dry place.
known by above analysis, drug effect of the present invention is outstanding, has the splendid effect of curing the disease.
More than show and described ultimate principle of the present invention, principal character and advantage of the present invention.Those skilled in the art should understand the present invention and not be restricted to the described embodiments; that in above-described embodiment and description, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in claimed scope.
Claims (4)
1. a Chinese medicine preparation for QI invigorating tonifying YANG and benefiting essence and marrow, is characterized in that, its raw material by following components by weight percent is prepared from: Radix Ginseng 90-140 part; Radix Morindae Officinalis 40-80 part; Cornu Cervi Pantotrichum 15-25 part; Herba Cistanches 50-70 part; Hippocampus 50-70 part; Cortex Eucommiae 50-70 part; Fossilia Spiriferis 50-70 part; Herba Epimedii 100-140 part; Flos Caryophylli 15-25 part; Semen Impatientis 15-25 part; Gecko 15-25 part; Fructus Corni 100-130 part; Fructus Cnidii 100-135 part; Concha Ostreae 100-135 part; Radix Astragali 900-1050 part; Pheretima 40-60 part; Cortex Cinnamomi 40-60 part; Fructus Schisandrae Chinensis 40-65 part.
2. the Chinese medicine preparation of a kind of QI invigorating tonifying YANG as claimed in claim 1 and benefiting essence and marrow, is characterized in that, its raw material by following components by weight percent is prepared from: 120 parts of Radix Ginsengs; 60 parts of Radix Morindae Officinaliss; 20 parts, Cornu Cervi Pantotrichum; 60 parts of Herba Cistanches; 60 parts of Hippocampus; 60 parts of the Cortexs Eucommiae; 60 parts, Fossilia Spiriferis; 120 parts of Herba Epimedii; 20 parts of Flos Caryophyllis; 20 parts of Semen Impatientis; 20 parts of Geckos; 120 parts of Fructus Corni; 120 parts of Fructus Cnidiis; 120 parts of Concha Ostreaes; 1000 parts of the Radixs Astragali; 60 parts of Pheretimas; 60 parts of Cortex Cinnamomis; 60 parts of Fructus Schisandrae Chinensis.
3. prepare the method for the preparation as described in claim 1 or 2, it is characterized in that: comprise following steps:
● just make: Radix Ginseng, Cortex Cinnamomi, Flos Caryophylli, the Cortex Eucommiae are dried, and are ground into powder, cross 60 mesh sieves, mix with Fructus Cnidii;
● extraction and concentration: add 80% alcohol reflux twice, add for the first time alcohol 4 volumes and doubly measure, extract 8 hours, add for the second time alcohol 2 volumes and doubly measure, extract merge extractive liquid, 6 hours, filter, decompression filtrate recycling ethanol, and be concentrated into the extractum of relative density 1.30～1.34 (80 ℃);
● decoct and concentrate: medicinal residues and Herba Cistanches, Radix Morindae Officinalis, Concha Ostreae, Fossilia Spiriferis, Fructus Schisandrae Chinensis, Semen Impatientis, Herba Epimedii, Fructus Corni, the Radix Astragali decoct with water three times, adding for the first time water 10 volumes doubly measures, decoct 2 hours, add for the second time water 8 volumes and doubly measure, decoct 2 hours, add for the third time water 6 volumes and doubly measure, decoct 1 hour, collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃); Cornu Cervi Pantotrichum, Hippocampus, Gecko, Pheretima are dried, pulverize, decoct with water twice, add for the first time water 8 volumes and doubly measure, add for the second time water 6 volumes and doubly measure, decoct respectively 2 hours, collecting decoction, filters, and filtrate is concentrated into the extractum of relative density 1.30～1.34 (80 ℃);
● regulate PH and concentrated: medicinal residues add water, add hydrochloric acid and regulate pH value to 4.5～5.0, in 100 ℃ of ordinary-pressure hydrolysis 24 hours, filter, filtrate adds 10% sodium hydroxide solution and regulates pH value to 6.5～7.0, filters, and filtrate decompression is concentrated into the extractum of relative density 1.30～1.34 (80 ℃).
4. the preparation method of preparation as claimed in claim 3, is characterized in that: also comprise the step of granulation, merge extractum, mix, add starch, mix, dry, pulverize into fine powder, with sucrose, add ethanol granulation, be drying to obtain.
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| CN104522236A (en) * | 2014-12-19 | 2015-04-22 | 青岛崂好人海洋生物技术有限公司 | Method for preparing spirifer composite health tea pills |
| CN104678045A (en) * | 2014-12-18 | 2015-06-03 | 南方医科大学 | Method for detecting radix sophorae flavescentis-eucalyptus robusta rash-eliminating gel medicine |
| CN105147838A (en) * | 2015-09-30 | 2015-12-16 | 北华大学 | Composition with immunity-enhancing and fatigue-resisting effects and preparation method and application thereof |
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| Publication number | Publication date |
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| CN103655821B (en) | 2016-05-25 |
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