CN103645087A - Phosphate buffer - Google Patents
Phosphate buffer Download PDFInfo
- Publication number
- CN103645087A CN103645087A CN201310559261.2A CN201310559261A CN103645087A CN 103645087 A CN103645087 A CN 103645087A CN 201310559261 A CN201310559261 A CN 201310559261A CN 103645087 A CN103645087 A CN 103645087A
- Authority
- CN
- China
- Prior art keywords
- sulfuric acid
- dilute sulfuric
- phosphate buffer
- parts
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000008363 phosphate buffer Substances 0.000 title claims abstract description 10
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims abstract description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000011780 sodium chloride Substances 0.000 claims abstract description 5
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 238000003756 stirring Methods 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 239000008267 milk Substances 0.000 claims description 3
- 210000004080 milk Anatomy 0.000 claims description 3
- 235000013336 milk Nutrition 0.000 claims description 3
- 239000001488 sodium phosphate Substances 0.000 claims description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 3
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 3
- 239000000243 solution Substances 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000013270 controlled release Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- DCFLORDWVMNDOH-UHFFFAOYSA-K O.O.O.O.P(=O)([O-])([O-])[O-].[Na+].[Na+].[Na+] Chemical compound O.O.O.O.P(=O)([O-])([O-])[O-].[Na+].[Na+].[Na+] DCFLORDWVMNDOH-UHFFFAOYSA-K 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000000839 emulsion Substances 0.000 abstract 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 abstract 1
- 238000009775 high-speed stirring Methods 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 7
- 239000010452 phosphate Substances 0.000 description 7
- 239000002953 phosphate buffered saline Substances 0.000 description 7
- 239000008366 buffered solution Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
Landscapes
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a preparation method of a phosphate buffer. The method particularly comprises the following steps: preparing raw materials of 1-3 parts of sodium chloride, 2-10 parts of distilled water, and 3-10 parts of sodium phosphate tetrahydrate; mixing the above components, adding dilute sulfuric acid, adjusting the pH value to 7.2; performing autoclaved sterilization, then storing at a low temperature; adding a ferroferric oxide solution into the mixed solution, mechanically stirring with a high speed; removing the supernatant; forming an emulsion, cooling again; and storing the product at room temperature. The mass fraction of the dilute sulfuric acid is not more than 30%; high speed stirring is necessary for complete turbidity before the addition of the dilute sulfuric acid. The beneficial effects of the invention are that the operation is simple, the reaction condition is mild, no toxic organic solvent is used, influence factors are few, and very good controlled release effect is realized.
Description
Technical field
The present invention relates to a kind ofly to chemical industry preparation technology, specifically, refer to phosphate buffer.
Background technology
Phosphate buffer (Phosphate Buffered Saline, be called for short PBS) be a buffer solution for biological study of commonly using.PBS can be the english abbreviation of three kinds of solution, respectively phosphate buffered solution (phosphate buffered solution), phosphate buffered saline (PBS) (phosphate buffered saline) and phosphate-buffered sodium (phosphate buffered sodium), its compound method is different, pH value is different, and the biological action of performance is also incomplete same.If no special instructions, biologically conventional PBS is neutral phosphate buffered solution (phosphate buffered solution).It is to contain sodium chloride in a kind of water base salt solusion, phosphate, and (at some formula) potassium chloride and potassium phosphate.Damping fluid contributes to the pH value that keeps constant.The osmotic pressure of solution and ion concentration be close with human body pH (wait and ooze) conventionally.Be mainly used in not affecting in the situation of testing reaction and regulating pH value in some chemical experiments, to allow the chemical reaction of experiment carry out under top condition.Part is for food or feedstuff industry processing, in food or the check of feed mycotoxin
elisa method wash the dilution buffer liquid in plate application or food microorganisms check.
Summary of the invention
For overcoming above-mentioned technical matters, we have proposed following technical scheme:
The collocation method of phosphate buffer, concrete steps are:
Prepare raw material: by sodium chloride: 1-3 part, distilled water 2-10 part, four water and sodium phosphate 3-10 part;
After mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
After autoclaving, under low temperature, preserve;
To in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
Abandon supernatant;
Form milk, again cold putting;
Under room temperature, preserve.
In the present invention, the massfraction of described dilute sulfuric acid is not higher than 30 percent.
In the present invention, add dilute sulfuric acid to need high-speed stirred before, make it completely muddy.
The invention has the beneficial effects as follows, simple to operate, reaction conditions coincide, and does not use poisonous organic solvent, and influence factor is less, can obtain good controlled-release effect.
Embodiment
The collocation method of phosphate buffer, concrete steps are:
Prepare raw material: by sodium chloride: 0 part of 2 parts, 10 parts of distilled water, four water and sodium phosphate;
After mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
After autoclaving, under low temperature, preserve;
To in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
Abandon supernatant;
Form milk, again cold putting;
Under room temperature, preserve.
The massfraction of described dilute sulfuric acid 30 percent.
Before adding dilute sulfuric acid, need high-speed stirred, make it completely muddy.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (3)
1. the collocation method of phosphate buffer, is characterized in that:
1) prepare raw material: by sodium chloride: 1-3 part, distilled water 2-10 part, four water and sodium phosphate 3-10 part;
2) after mentioned component is mixed, add dilute sulfuric acid, its pH value is adjusted to 7.2;
3), after autoclaving, under low temperature, preserve;
4) will in solution, add tri-iron tetroxide solution, then mechanical high-speed stirs;
5) abandon supernatant;
6) form milk, again cold putting;
7) under room temperature, preserve.
2. the collocation method of phosphate buffer as claimed in claim 1, is characterized in that, the massfraction of described dilute sulfuric acid is not higher than 30 percent.
3. the collocation method of phosphate buffer as claimed in claim 1, is characterized in that, adds dilute sulfuric acid to need high-speed stirred before, makes it completely muddy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310559261.2A CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310559261.2A CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103645087A true CN103645087A (en) | 2014-03-19 |
Family
ID=50250339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310559261.2A Pending CN103645087A (en) | 2013-11-12 | 2013-11-12 | Phosphate buffer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103645087A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426761A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of phosphate buffer and preparation method thereof |
| CN108426762A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of configuration method of concentrate phosphate buffer |
| CN109307770A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose calibration solution configuration method |
| CN109307777A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | It is a kind of suitable for glucose/lactic acid analysis instrument blood glucose calibration solution configuration method |
| CN109307769A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose solution allocation method for 12mmol/L |
-
2013
- 2013-11-12 CN CN201310559261.2A patent/CN103645087A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108426761A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of phosphate buffer and preparation method thereof |
| CN108426762A (en) * | 2017-02-15 | 2018-08-21 | 上海瀚联医疗技术股份有限公司 | A kind of configuration method of concentrate phosphate buffer |
| CN108426761B (en) * | 2017-02-15 | 2021-11-05 | 上海瀚联医疗技术股份有限公司 | Phosphate buffer solution and preparation method thereof |
| CN108426762B (en) * | 2017-02-15 | 2021-11-30 | 上海瀚联医疗技术股份有限公司 | Preparation method of concentrated solution phosphate buffer solution |
| CN109307770A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose calibration solution configuration method |
| CN109307777A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | It is a kind of suitable for glucose/lactic acid analysis instrument blood glucose calibration solution configuration method |
| CN109307769A (en) * | 2017-07-28 | 2019-02-05 | 上海瀚联医疗技术股份有限公司 | A kind of blood glucose solution allocation method for 12mmol/L |
| CN109307770B (en) * | 2017-07-28 | 2022-06-17 | 上海瀚联医疗技术股份有限公司 | Blood sugar calibration solution preparation method |
| CN109307777B (en) * | 2017-07-28 | 2022-06-17 | 上海瀚联医疗技术股份有限公司 | Blood glucose calibration solution preparation method suitable for glucose/lactic acid analyzer |
| CN109307769B (en) * | 2017-07-28 | 2022-06-28 | 上海瀚联医疗技术股份有限公司 | Preparation method of 12mmol/L blood glucose solution |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140319 |
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| WD01 | Invention patent application deemed withdrawn after publication |