CN103642685A - Large-scale cell culture bottle - Google Patents
Large-scale cell culture bottle Download PDFInfo
- Publication number
- CN103642685A CN103642685A CN201310525786.4A CN201310525786A CN103642685A CN 103642685 A CN103642685 A CN 103642685A CN 201310525786 A CN201310525786 A CN 201310525786A CN 103642685 A CN103642685 A CN 103642685A
- Authority
- CN
- China
- Prior art keywords
- box
- middle box
- upper cover
- cell culture
- end outer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a large-scale cell culture bottle. According to the large-scale cell culture bottle, a multilayer cell culture space is arranged between a base box and an upper cover so that a manufacturing cost is low and a cell culture space benefit is high. The large-scale cell culture bottle comprises the base box and the upper cover. Two sides of one end of the upper cover are provided with pouring openings. The large-scale cell culture bottle is characterized in that at least four layers of middle boxes are arranged between the base box and the upper cover; an outer edge bulge of the lower end of the upper cover is inlaid in an outer edge sealing groove of the upper end of the top middle box; an outer edge bulge of the lower end of each upper middle box is inlaid in an outer edge sealing groove of the upper end of the adjacent middle box below the upper middle box; an outer edge bulge of the lower end of the bottom middle box is inlaid in an outer edge sealing groove of the upper end of the base box; the bottom of each one of the middle boxes is provided with a division plate; a side of the division plate extending to the pouring openings is provided with a longitudinal barrier strip; the height of the longitudinal barrier strip is less than the height of the middle box; and a cavity formed between the longitudinal barrier strip of the middle box and a side edge at the side is a communication cavity.
Description
Technical field
The present invention relates to the technical field of cell culture apparatus, be specially a kind of mass cell culturing bottle.
Background technology
Existing Tissue Culture Flask, box, a upper cover at the bottom of it comprises one, Tissue Culture Flask can only arrange the cell culturing space of one deck, when needs are cultivated a large amount of cells, need a plurality of Tissue Culture Flasks, and then cause the corresponding a plurality of upper covers of box of a plurality of ends to form a plurality of cell culturing spaces, it makes manufacturing cost high, and cell culturing space can not produce maximum utilization ratio, make cell culturing space benefit low.
Summary of the invention
For the problems referred to above, the invention provides a kind of mass cell culturing bottle, the cell culturing space that it makes to be provided with between box at the bottom of, a upper cover multilayer, makes the high efficiency of low cost of manufacture, cell culturing space.
A kind of mass cell culturing bottle, its technical scheme is such: it comprises end box, upper cover, the both sides, one end of described upper cover have perfusing hole, it is characterized in that: box of the described end, between upper cover, be provided with box at least four layers, the lower end outer rim projection of described upper cover is flush-mounted in the upper end outer fringe seal groove of described middle box of last layer, the lower end outer rim projection of the described middle box on upper strata is flush-mounted in the upper end outer fringe seal groove of described middle box of its adjacent lower one deck, the lower end outer rim projection of the described middle box of basecoat is flush-mounted in the upper end outer fringe seal groove of box of the described end, the bottom of described middle box is provided with dividing plate, the side that described dividing plate extends near described perfusing hole is provided with longitudinal blend stop, the height of described longitudinal blend stop is less than the height of described middle box, cavity corresponding between longitudinal blend stop of described middle box and the side of this side is connected chamber, at the bottom of box corresponding to described connected chamber, position has communicating aperture, described communicating aperture is pressed close to the side of this side, described communicating aperture is communicated with adjacent described connected chamber, and described perfusing hole is communicated with the connected chamber of the described middle box of last layer.
Use after structure of the present invention, due to end box, between upper cover, be provided with box at least four layers, and the bottom of middle box is provided with dividing plate, the side that dividing plate extends near described import is provided with longitudinal blend stop, its hypothesis has box in N layer, make end box, between upper cover, be divided into (N+1) individual cell culturing space, box at the bottom of it makes one, between a upper cover, be provided with the cell culturing space of multilayer, during use, cell culture fluid is poured into from perfusing hole, and then two perfusing holes are sealed, again by perfusing hole down, uprightly put, between every one deck due to product, by communicating aperture, be communicated with, nutrient solution will flow uniformly in every one deck, again by product flip vertical, between box, to flow into corresponding dividing plate inner in gap in longitudinal blend stop and upper strata to make nutrient solution, the cell culture fluid of filling with is layered on the baseplane of every one deck uniformly, the cultivation of carrying out cell makes low cost of manufacture, the high efficiency of cell culturing space.
Accompanying drawing explanation
Fig. 1 is stereographic map structural representation of the present invention;
The structural representation sectional view that Fig. 2 is this realistic scale when placing;
Fig. 3 is the local structure for amplifying schematic diagram in the A place of Fig. 2.。
Embodiment
See Fig. 1-3, it comprises end box 1, upper cover 2, the both sides, one end of upper cover 2 have perfusing hole 3, end box 1, between upper cover 2, be provided with box 4 at least four layers, the lower end outer rim projection 5 of upper cover 2 is flush-mounted in the upper end outer fringe seal groove 6 of middle box 4 of last layer, the lower end outer rim projection 5 of the middle box 4 on upper strata is flush-mounted in the upper end outer fringe seal groove 6 of middle box 4 of its adjacent lower one deck, the lower end outer rim projection 5 of the middle box 4 of basecoat is flush-mounted in the upper end outer fringe seal groove 6 of end box 1, the bottom of middle box 4 is provided with dividing plate 7, the side that dividing plate 7 extends near perfusing hole 3 is provided with longitudinal blend stop 8, longitudinally the height of blend stop 8 is less than the height of middle box 4, longitudinal blend stop 8 of middle box 4 and 9 corresponding cavitys of the side of this side are connected chamber 10, at the bottom of the box of connected chamber 10 correspondences, position has communicating aperture 11, communicating aperture 11 is pressed close to the side 9 of this side, and communicating aperture 11 is communicated with adjacent connected chamber 10, and perfusing hole 3 is communicated with the connected chamber 10 of the middle box 4 of last layer.Between end box 1, middle box 4, interconnective each layer of upper cover 2, with ultrasonic welding, weld, guarantee the stopping property of product.In figure, 12 is ultrasonic weld seam, and 13 is nutrient solution.
Claims (1)
1. a mass cell culturing bottle, it comprises end box, upper cover, the both sides, one end of described upper cover have perfusing hole, it is characterized in that: box of the described end, between upper cover, be provided with box at least four layers, the lower end outer rim projection of described upper cover is flush-mounted in the upper end outer fringe seal groove of described middle box of last layer, the lower end outer rim projection of the described middle box on upper strata is flush-mounted in the upper end outer fringe seal groove of described middle box of its adjacent lower one deck, the lower end outer rim projection of the described middle box of basecoat is flush-mounted in the upper end outer fringe seal groove of box of the described end, the bottom of described middle box is provided with dividing plate, the side that described dividing plate extends near described perfusing hole is provided with longitudinal blend stop, the height of described longitudinal blend stop is less than the height of described middle box, cavity corresponding between longitudinal blend stop of described middle box and the side of this side is connected chamber, at the bottom of box corresponding to described connected chamber, position has communicating aperture, described communicating aperture is pressed close to the side of this side, described communicating aperture is communicated with adjacent described connected chamber, and described perfusing hole is communicated with the connected chamber of the described middle box of last layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525786.4A CN103642685A (en) | 2013-10-31 | 2013-10-31 | Large-scale cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525786.4A CN103642685A (en) | 2013-10-31 | 2013-10-31 | Large-scale cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103642685A true CN103642685A (en) | 2014-03-19 |
Family
ID=50247973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310525786.4A Pending CN103642685A (en) | 2013-10-31 | 2013-10-31 | Large-scale cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103642685A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087509A (en) * | 2014-07-11 | 2014-10-08 | 上海圣纳堡生物科技开发有限公司 | Multilayer cell culture container |
| CN105524832A (en) * | 2016-02-26 | 2016-04-27 | 广州洁特生物过滤股份有限公司 | Cell culture device and method |
| CN105586260A (en) * | 2016-03-11 | 2016-05-18 | 阜阳师范学院 | Layered cell coculturing device and method |
-
2013
- 2013-10-31 CN CN201310525786.4A patent/CN103642685A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104087509A (en) * | 2014-07-11 | 2014-10-08 | 上海圣纳堡生物科技开发有限公司 | Multilayer cell culture container |
| CN105524832A (en) * | 2016-02-26 | 2016-04-27 | 广州洁特生物过滤股份有限公司 | Cell culture device and method |
| CN105524832B (en) * | 2016-02-26 | 2018-03-30 | 广州洁特生物过滤股份有限公司 | Cell culture apparatus and method |
| US11028356B2 (en) | 2016-02-26 | 2021-06-08 | Guangzhou Jet Bio-Filtration Co., Ltd. | Cell culture apparatus and method |
| CN105586260A (en) * | 2016-03-11 | 2016-05-18 | 阜阳师范学院 | Layered cell coculturing device and method |
| CN105586260B (en) * | 2016-03-11 | 2019-01-22 | 阜阳师范学院 | A kind of device and method that cell layering co-cultures |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140319 |