CN103630649A - Method for analyzing and detecting dihydroxyacetone - Google Patents
Method for analyzing and detecting dihydroxyacetone Download PDFInfo
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- CN103630649A CN103630649A CN201310580495.5A CN201310580495A CN103630649A CN 103630649 A CN103630649 A CN 103630649A CN 201310580495 A CN201310580495 A CN 201310580495A CN 103630649 A CN103630649 A CN 103630649A
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- dihydroxyacetone
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Abstract
The invention discloses a method for analyzing and detecting dihydroxyacetone. The method is specifically characterized by comprising the steps of firstly, carrying out qualitative analysis on dihydroxyacetone by using a thin-layer chromatography, and repeatedly testing; then, converting dihydroxyacetone into a derivative which has relatively high thermal stability and can be still not changed at the temperature of about 80 DEG C; detecting at the temperature of 250 DEG C, and meanwhile, gradually increasing column temperature to 320 DEG C from 15 DEG C within an hour; taking nitrogen gas as carrier gas by adopting the split ratio of (10-20): 1; finally, carrying out derived separation and detection on a moving phase through trimethyl ethanol as a wavelength treatment solution. The concentration of trimethyl ethanol is 5% to 10%; during test, used heat is supplied by heat generated through the oxidation of lactose oxidase. The method has the beneficial effects that the conditions are stable, the operation is simple and easy, the method can be applied to mass production and is adaptable to industrial requirements, and the purity is relatively high.
Description
Technical field
The present invention relates to a kind of experimental technique, specifically, refer to the analyzing and testing method to dihydroxyacetone.
Background technology
Dihydroxyacetone (1,3-Dihydroxyacetone) or 1,3-dihydroxyacetone (Dihydroxyacetone), is called for short DHA, is polyhydroxy ketose, it is the simplest ketose, outward appearance is with pleasantly sweet white powder crystallization, soluble in water, ethanol, ether and acetone and other organic solvent, and fusing point is 75-80 ℃, water-soluble >250g/L (20 ℃) is to stablize for 6.0 o'clock at pH.Dihydroxyacetone is the non-reducing sugar in monose, and without unsymmetrical carbon, therefore without optical activity.Dihydroxyacetone is as a kind of good active coloring components, and sun-proof property is extremely strong, and approximately 50% dihydroxyacetone product, in the middle of cosmetics, is made into the splendid skin lotion of various effects in the world.Dihydroxy the third heir can only infiltrate into the cuticula of skin surface, but cannot arrive skin corium, so can not cause damage to skin, it is harmless that human body is used.Dihydroxyacetone is by being combined with the free amino acid of skin surface; at skin surface, form the diaphragm of one deck densification; stop moisture of skin excessive vaporization, can effectively avoid the irradiation damage of ultraviolet ray and visible ray, thereby play good moisturizing and anti-sunlight function.Dihydroxyacetone is for imitative solarization agent, different from ultraviolet ray irradiation generation Mai Laning melanin, can make the colour of skin become bronze-colored, and can reduce the damage that ultraviolet Irradiation on Skin causes.The current domestic report of not yet realizing suitability for industrialized production, comprise that the states such as the U.S., Britain, Japan, Germany are at the 60 to 70's of last century large-scale industrialization fermenting and producing dihydroxyacetone just abroad, and two benzylacetones have obtained the production method of practical application dihydroxyacetone widely chemical method and biotransformation method, industrial is mainly to rely on bioanalysis large-scale production dihydroxyacetone.At present, the focus of research mainly concentrates on Production by Microorganism Fermentation dihydroxyacetone both at home and abroad.
Summary of the invention
For overcoming above-mentioned technical matters, we have proposed following technical scheme:
Analyzing and testing method to dihydroxyacetone:
First, use thin-layered chromatography to carry out qualitative analysis to it, repeatedly test;
Then be translated into thermal stability higher, the derivant that still should not change 80 degrees Celsius of left and right;
The temperature detecting is 250 degrees Celsius, and the temperature of column temperature, from 15 degree, in one hour, rises to 320 degrees Celsius gradually simultaneously;
Using nitrogen as carrier gas, split ratio is 10-20:1;
Finally pass through front three ethanol as wavelength treating fluid, it entered to mobile phase and carry out the separated detection of derivatization.
In the present invention, the concentration of described front three ethanol is 5 percent to 10.
In the present invention, in experimentation, the heat of use, is that the heat being produced by lactose oxidation oxydasis obtains.
The invention has the beneficial effects as follows, conditional stability, suggestion for operation, can adapt to industrialization demand for batch production, and purity is higher.
Embodiment
Analyzing and testing method to dihydroxyacetone:
First, use thin-layered chromatography to carry out qualitative analysis to it, repeatedly test;
Then be translated into thermal stability higher, the derivant that still should not change 80 degrees Celsius of left and right;
The temperature detecting is 250 degrees Celsius, and the temperature of column temperature, from 15 degree, in one hour, rises to 320 degrees Celsius gradually simultaneously;
Using nitrogen as carrier gas, split ratio is 10:1;
Finally pass through front three ethanol as wavelength treating fluid, it entered to mobile phase and carry out the separated detection of derivatization.
In the present invention, the concentration of described front three ethanol is 5 percent.
In the present invention, in experimentation, the heat of use, is that the heat being produced by lactose oxidation oxydasis obtains.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (3)
1. the analyzing and testing method of pair dihydroxyacetone, is characterized in that:
1) first, use thin-layered chromatography to carry out qualitative analysis to it, repeatedly test;
2) be then translated into thermal stability higher, the derivant that still should not change 80 degrees Celsius of left and right;
3) temperature detecting is 250 degrees Celsius, and the temperature of column temperature, from 15 degree, in one hour, rises to 320 degrees Celsius gradually simultaneously;
4), using nitrogen as carrier gas, split ratio is 10-20:1;
5) finally pass through front three ethanol as wavelength treating fluid, it entered to mobile phase and carry out the separated detection of derivatization.
2. the analyzing and testing method to dihydroxyacetone as claimed in claim 1, is characterized in that, the concentration of described front three ethanol is 5 percent to 10.
3. the analyzing and testing method to dihydroxyacetone as claimed in claim 1, is characterized in that, in experimentation, the heat of use, is that the heat being produced by lactose oxidation oxydasis obtains.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310580495.5A CN103630649A (en) | 2013-11-19 | 2013-11-19 | Method for analyzing and detecting dihydroxyacetone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310580495.5A CN103630649A (en) | 2013-11-19 | 2013-11-19 | Method for analyzing and detecting dihydroxyacetone |
Publications (1)
| Publication Number | Publication Date |
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| CN103630649A true CN103630649A (en) | 2014-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310580495.5A Pending CN103630649A (en) | 2013-11-19 | 2013-11-19 | Method for analyzing and detecting dihydroxyacetone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112424597A (en) * | 2018-07-27 | 2021-02-26 | 株式会社岛津制作所 | Analysis device |
-
2013
- 2013-11-19 CN CN201310580495.5A patent/CN103630649A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112424597A (en) * | 2018-07-27 | 2021-02-26 | 株式会社岛津制作所 | Analysis device |
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Application publication date: 20140312 |
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| WD01 | Invention patent application deemed withdrawn after publication |