CN103627762A - Method for extracting protein polypeptide from Onchidium struma by virtue of enzymolysis - Google Patents
Method for extracting protein polypeptide from Onchidium struma by virtue of enzymolysis Download PDFInfo
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- CN103627762A CN103627762A CN201310516990.XA CN201310516990A CN103627762A CN 103627762 A CN103627762 A CN 103627762A CN 201310516990 A CN201310516990 A CN 201310516990A CN 103627762 A CN103627762 A CN 103627762A
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- protein polypeptide
- onchidium struma
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Abstract
The invention discloses a method for extracting protein polypeptide from Onchidium struma by virtue of enzymolysis. The method is carried out according to the following steps: firstly, smashing dried Onchidium struma, proportionally adding distilled water to soak the smashed Onchidium struma into liquid; then, regulating pH value of the liquid and proportionally adding papain, and stirring well; heating up the liquid to 50-60 DEG C to hydrolyze protein, heating up after hydrolyzing protein to execute enzyme deactivation, cooling naturally to room temperature, centrifuging the liquid in a high speed centrifuge to obtain supernate, and proportionally adding powdered activated carbon, stirring well, heating up to 50-60 DEG C, and filtering to remove the powdered activated carbon after preserving heat for a period of time; next, proportionally adding beta-cyclodextrine, stirring well and heating up to 65-70 DEG C, preserving heat, and then, cooling naturally to room temperature to obtain the liquid-state protein polypeptide; and finally, concentrating the liquid-state protein polypeptide under vacuum low temperature condition, and performing vacuum freeze drying, thereby obtaining the protein polypeptide finished product. According to the method, technological process is simple, formula is scientific and reasonable, and the prepared protein polypeptide has a health maintenance effect.
Description
Technical field
The present invention relates to sea-food development of resources technical field, specifically a kind of method that adopts Onchidium struma enzymolysis and extraction protein polypeptide.
Background technology
Growth, growth, immunomodulatory and the metabolism of polypeptide major control human body.Polypeptide makes human body in a kind of equilibrium state, if after bioactive peptide minimizing, and the function generation important change of human body, for children, his growth, growth become slowly, even stop, and go down for a long time just to have formed dwarf; To grownup or the elderly, lack after bioactive peptide, the immunizing power of self will decline, metabolic disturbance, endocrine disturbance, the generation giving rise to diseases, as insomnia, health is become thin or edema.Because bioactive peptide also acts on neural system, thus the human body slow movement that will become, brains is no longer wise, and main is that bioactive peptide reduces, and directly causes that each position of the person engenders comprehensive aging, causes various diseases.
Onchidium struma (Onchidium struma) (abbreviation orpiment), is subordinate to Mollusca, Gastropoda, Pulmonata, Onchidiidae; The climax band beach ，Shi circle Yu Haiyang of the degree of saltiness water intersection on the ground such as normal distribution China Jiangsu, Shanghai, Zhejiang, Fujian, Guangdong, Hainan and the animal of land transitional zone, health is oval, exposed without shell, is considered to a kind of shellfish of evolution.Before the eighties in 20th century, the natural resources quantity of China's Onchidium struma is very huge, is even often used as feed, the little seafood how to develop as coastal fisherman.China southeastern coast human consumer, as high-grade seafood and nutritional supplements, has long edible history; The main essential amino acids content of Yancheng, Jiangsu Province characteristic sea-food Onchidium struma is higher than abalone, and protein content is higher than oyster, and its meat flavour is delicious, is rich in multiple amino acids and trace element, is deeply subject to human consumer's extensive welcome.As a kind of edible with a long history, newly-developed edible seafood rapidly, although orpiment shared share in fishery products is little, even cannot in fisheries statistics data, find its position, but on Yancheng, Jiangsu Province (being commonly called as extra large favus), Wenzhou District of Zhejiang Province (be commonly called as and be coated with tortoise), Bay, Ningde, Fujian Province (being commonly called as native Bao) and other places, form market widely, popular legend has nourishing and treats rheumatismal function, also be the delicious speciality together (as the green bamboo shoot soil Bao soup in Ningde) in these areas, local resident's edible history is longer.
Summary of the invention
The object of the invention is the defect existing for prior art, provide a kind of scientific formulation, technique simply to adopt the method for Onchidium struma enzymolysis and extraction protein polypeptide.
The object of the invention is to solve by the following technical programs:
Adopt a method for Onchidium struma enzymolysis and extraction protein polypeptide, it is characterized in that described method carries out in the steps below:
(a), will after the pulverizing of dry product Onchidium struma, add in proportion distilled water immersion to make feed liquid;
(b), the pH value of feed liquid is adjusted to 6.0-6.5, then in the 0.1-0.7%(W/V of feed liquid cumulative volume) ratio add papoid and stir;
(c), above-mentioned feed liquid is heated to 50-60 ℃, keep 5-10h protein hydrolysate, after proteolysis, be warming up to the 90-105 ℃ of enzyme that goes out, naturally cool to room temperature;
(d), the feed liquid of room temperature state is put into the centrifugal acquisition supernatant liquor of supercentrifuge, in the 0.3-0.8%(W/V of supernatant liquor cumulative volume) ratio heat to 50-60 ℃ after adding Powdered Activated Carbon to stir evenly, after insulation for some time, filter and remove Powdered Activated Carbon;
(e), then by the 1.3-2%(W/V of the supernatant liquor cumulative volume after filtering) ratio be warming up to 65-70 ℃ after adding cycloheptaamylose to stir evenly, be incubated and naturally cool to room temperature after 0.5-1h and obtain liquid protein polypeptide;
(f), finally adopt vacuum and low temperature to concentrate also the liquid protein polypeptide of above-mentioned acquisition and make protein polypeptide finished product after vacuum lyophilization.
In described step (a), the size of dry product Onchidium struma is pulverized as 20-30 order.
Dry product Onchidium struma in described step (a) and the ratio of distilled water are 1:8-15.
Onchidium struma in described step (a) soaks 2-3h and makes feed liquid.
The enzyme time of going out in described step (c) is 10-15min.
Soaking time in described step (d) is 15-30min.
The present invention has the following advantages compared to existing technology:
After first the present invention is crushed to dry product Onchidium struma a certain size, feed liquid is made in immersion, then add papain hydrolysis protein, centrifugal acquisition supernatant liquor after enzyme goes out, after supernatant liquor is carried out to add cycloheptaamylose to process after adsorption filtration, obtain liquid protein polypeptide, concentrated the and vacuum lyophilization of last vacuum and low temperature obtains protein polypeptide finished product; Whole technical process is simple, the starting material environmental protection health of employing, and scientific formulation is reasonable, and the protein polypeptide making has good health effect.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment mono-
A kind of method that adopts Onchidium struma enzymolysis and extraction protein polypeptide, first dry product Onchidium struma is ground into 20 object sizes, then, in dry product Onchidium struma: the ratio that distilled water is 1:8 adds the dry product Onchidium struma after pulverizing in distilled water and soaks, after immersion 2h, make feed liquid; The pH value of feed liquid is adjusted to 6.0, then in the 0.2%(W/V of feed liquid cumulative volume) ratio add papoid and stir; The feed liquid that adds papoid heated to 50-60 ℃ and kept 6h protein hydrolysate, after proteolysis, be warming up to 90 ℃, keeping 15min to go out after enzyme and naturally cool to room temperature; The feed liquid of room temperature state is put into the centrifugal acquisition supernatant liquor of supercentrifuge, in the 0.4%(W/V of supernatant liquor cumulative volume) ratio heat to 50 ℃ after adding Powdered Activated Carbon to stir evenly, filter residue is dried and be can be used for other purposes, after supernatant liquor insulation 15min, filters and removes Powdered Activated Carbon; Then by the 1.3%(W/V of the supernatant liquor cumulative volume after filtering) ratio be warming up to 65 ℃ after adding cycloheptaamylose to stir evenly, be incubated and naturally cool to room temperature after 0.6h and obtain liquid protein polypeptide; Finally adopt vacuum and low temperature to concentrate also the liquid protein polypeptide of above-mentioned acquisition and make protein polypeptide finished product after vacuum lyophilization.After first the present invention is crushed to dry product Onchidium struma a certain size, feed liquid is made in immersion, then add papain hydrolysis protein, centrifugal acquisition supernatant liquor after enzyme goes out, after supernatant liquor is carried out to add cycloheptaamylose to process after adsorption filtration, obtain liquid protein polypeptide, concentrated the and vacuum lyophilization of last vacuum and low temperature obtains protein polypeptide finished product; Whole technical process is simple, the starting material environmental protection health of employing, and scientific formulation is reasonable, and the protein polypeptide making has good health effect.
Embodiment bis-
A kind of method that adopts Onchidium struma enzymolysis and extraction protein polypeptide, first dry product Onchidium struma is ground into 25 object sizes, then, in dry product Onchidium struma: the ratio that distilled water is 1:10 adds the dry product Onchidium struma after pulverizing in distilled water and soaks, after immersion 2.3h, make feed liquid; The pH value of feed liquid is adjusted to 6.3, then in the 0.5%(W/V of feed liquid cumulative volume) ratio add papoid and stir; The feed liquid that adds papoid heated to 50-60 ℃ and kept 8h protein hydrolysate, after proteolysis, be warming up to 95 ℃, keeping 13min to go out after enzyme and naturally cool to room temperature; The feed liquid of room temperature state is put into the centrifugal acquisition supernatant liquor of supercentrifuge, in the 0.5%(W/V of supernatant liquor cumulative volume) ratio heat to 55 ℃ after adding Powdered Activated Carbon to stir evenly, filter residue is dried and be can be used for other purposes, after supernatant liquor insulation 20min, filters and removes Powdered Activated Carbon; Then by the 1.6%(W/V of the supernatant liquor cumulative volume after filtering) ratio be warming up to 65 ℃ after adding cycloheptaamylose to stir evenly, be incubated and naturally cool to room temperature after 0.5h and obtain liquid protein polypeptide; Finally adopt vacuum and low temperature to concentrate also the liquid protein polypeptide of above-mentioned acquisition and make protein polypeptide finished product after vacuum lyophilization.After first the present invention is crushed to dry product Onchidium struma a certain size, feed liquid is made in immersion, then add papain hydrolysis protein, centrifugal acquisition supernatant liquor after enzyme goes out, after supernatant liquor is carried out to add cycloheptaamylose to process after adsorption filtration, obtain liquid protein polypeptide, concentrated the and vacuum lyophilization of last vacuum and low temperature obtains protein polypeptide finished product; Whole technical process is simple, the starting material environmental protection health of employing, and scientific formulation is reasonable, and the protein polypeptide making has good health effect.
Embodiment tri-
A kind of method that adopts Onchidium struma enzymolysis and extraction protein polypeptide, first dry product Onchidium struma is ground into 30 object sizes, then, in dry product Onchidium struma: the ratio that distilled water is 1:13 adds the dry product Onchidium struma after pulverizing in distilled water and soaks, after immersion 3h, make feed liquid; The pH value of feed liquid is adjusted to 6.5, then in the 0.6%(W/V of feed liquid cumulative volume) ratio add papoid and stir; The feed liquid that adds papoid heated to 50-60 ℃ and kept 9h protein hydrolysate, after proteolysis, be warming up to 100 ℃, keeping 10 to go out after enzyme and naturally cool to room temperature; The feed liquid of room temperature state is put into the centrifugal acquisition supernatant liquor of supercentrifuge, in the 0.8%(W/V of supernatant liquor cumulative volume) ratio heat to 60 ℃ after adding Powdered Activated Carbon to stir evenly, filter residue is dried and be can be used for other purposes, after supernatant liquor insulation 25min, filters and removes Powdered Activated Carbon; Then by the 1.8%(W/V of the supernatant liquor cumulative volume after filtering) ratio be warming up to 70 ℃ after adding cycloheptaamylose to stir evenly, be incubated and naturally cool to room temperature after 0.8h and obtain liquid protein polypeptide; Finally adopt vacuum and low temperature to concentrate also the liquid protein polypeptide of above-mentioned acquisition and make protein polypeptide finished product after vacuum lyophilization.After first the present invention is crushed to dry product Onchidium struma a certain size, feed liquid is made in immersion, then add papain hydrolysis protein, centrifugal acquisition supernatant liquor after enzyme goes out, after supernatant liquor is carried out to add cycloheptaamylose to process after adsorption filtration, obtain liquid protein polypeptide, concentrated the and vacuum lyophilization of last vacuum and low temperature obtains protein polypeptide finished product; Whole technical process is simple, the starting material environmental protection health of employing, and scientific formulation is reasonable, and the protein polypeptide making has good health effect.
Above embodiment only, for explanation technological thought of the present invention, can not limit protection scope of the present invention with this, every technological thought proposing according to the present invention, and any change of doing on technical scheme basis, within all falling into protection domain of the present invention; The technology that the present invention does not relate to all can be realized by prior art.
Claims (6)
1. adopt a method for Onchidium struma enzymolysis and extraction protein polypeptide, it is characterized in that described method carries out in the steps below:
(a), will after the pulverizing of dry product Onchidium struma, add in proportion distilled water immersion to make feed liquid;
(b), the pH value of feed liquid is adjusted to 6.0-6.5, then in the 0.1-0.7%(W/V of feed liquid cumulative volume) ratio add papoid and stir;
(c), above-mentioned feed liquid is heated to 50-60 ℃, keep 5-10h protein hydrolysate, after proteolysis, be warming up to the 90-105 ℃ of enzyme that goes out, naturally cool to room temperature;
(d), the feed liquid of room temperature state is put into the centrifugal acquisition supernatant liquor of supercentrifuge, in the 0.3-0.8%(W/V of supernatant liquor cumulative volume) ratio heat to 50-60 ℃ after adding Powdered Activated Carbon to stir evenly, after insulation for some time, filter and remove Powdered Activated Carbon;
(e), then by the 1.3-2%(W/V of the supernatant liquor cumulative volume after filtering) ratio be warming up to 65-70 ℃ after adding cycloheptaamylose to stir evenly, be incubated and naturally cool to room temperature after 0.5-1h and obtain liquid protein polypeptide;
(f), finally adopt vacuum and low temperature to concentrate also the liquid protein polypeptide of above-mentioned acquisition and make protein polypeptide finished product after vacuum lyophilization.
2. the method for employing Onchidium struma enzymolysis and extraction protein polypeptide according to claim 1, is characterized in that the size of dry product Onchidium struma in described step (a) is pulverized as 20-30 order.
3. the method for employing Onchidium struma enzymolysis and extraction protein polypeptide according to claim 1, is characterized in that dry product Onchidium struma in described step (a) and the ratio of distilled water are 1:8-15.
4. the method for employing Onchidium struma enzymolysis and extraction protein polypeptide according to claim 1, is characterized in that the Onchidium struma immersion 2-3h in described step (a) makes feed liquid.
5. the method for employing Onchidium struma enzymolysis and extraction protein polypeptide according to claim 1, is characterized in that the enzyme time of going out in described step (c) is 10-15min.
6. the method for employing Onchidium struma enzymolysis and extraction protein polypeptide according to claim 1, is characterized in that the soaking time in described step (d) is 15-30min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105200107A (en) * | 2015-10-10 | 2015-12-30 | 上海海洋大学 | Method for extracting muscle crude protein of onchidium struma |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1082338A (en) * | 1993-03-22 | 1994-02-23 | 占康林 | From spiral shell, shellfish and freshwater mussel, produce the process of amino acid composite liquid |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1082338A (en) * | 1993-03-22 | 1994-02-23 | 占康林 | From spiral shell, shellfish and freshwater mussel, produce the process of amino acid composite liquid |
Non-Patent Citations (1)
| Title |
|---|
| 黄金田，等: "瘤背石磺营养成分分析及品质评价", 《海洋科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105200107A (en) * | 2015-10-10 | 2015-12-30 | 上海海洋大学 | Method for extracting muscle crude protein of onchidium struma |
| CN105200107B (en) * | 2015-10-10 | 2018-12-25 | 上海海洋大学 | The extracting method of Onchidium struma muscle crude protein |
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Application publication date: 20140312 |