CN103626531A - Compound fertilizer and preparation method thereof - Google Patents
Compound fertilizer and preparation method thereof Download PDFInfo
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- CN103626531A CN103626531A CN201310589442.XA CN201310589442A CN103626531A CN 103626531 A CN103626531 A CN 103626531A CN 201310589442 A CN201310589442 A CN 201310589442A CN 103626531 A CN103626531 A CN 103626531A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 150000001875 compounds Chemical class 0.000 title abstract description 7
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 241001513093 Aspergillus awamori Species 0.000 claims abstract description 13
- 239000000084 colloidal system Substances 0.000 claims abstract description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 26
- 239000002068 microbial inoculum Substances 0.000 claims description 14
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 13
- 229910052698 phosphorus Inorganic materials 0.000 claims description 13
- 239000011574 phosphorus Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 239000002131 composite material Substances 0.000 claims description 11
- 241000186660 Lactobacillus Species 0.000 claims description 10
- 229940039696 lactobacillus Drugs 0.000 claims description 10
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- 230000007480 spreading Effects 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 3
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- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 1
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- 102000015439 Phospholipases Human genes 0.000 description 1
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
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- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a compound fertilizer and a preparation method thereof. The compound fertilizer is composed of the following ingredients in parts by weight: 5-10 parts of a yeast agent, 5-10 parts of a colloid bacillus microbial agent, 5-10 parts of a phosphors-resolving bacillus-subtilis microbial agent, 10-15 parts of bacillus subtilis culture, 1-5 parts of aspergillus awamori culture and 1-4 parts of a lactobacillus plantarum agent. The fertilizer provided by the invention is rich in nutrition and high in organic matter content. The compound fertilizer provided by the invention is capable of meeting the production requirements of organic food. The fertilizer provided by the invention is formed by fermenting compound microorganisms, and thus is capable of improving the micro-ecological environment of soil and proving good conditions for the growth of crops.
Description
Technical field
The present invention relates to a kind of composite fertilizer, belong to agricultural fertilizer technical field.
Background technology
The application of chemical fertilizer plays an important role to agriculture volume increase, yet due to long-term application chemical fertilizer, fertilizer is not enough, and in soil, various nutrients ratio imbalances, cause farmland ecological environment, soil physical and chemical property and soil microflora to be subject to destruction in various degree.Also affected to a certain extent the quality of agricultural-food, " fruit is sweetless, and melon is not fragrant " is exactly the performance of this situation simultaneously.Pollution by chemical fertilizer has become the large public hazards in the world today one.Agricultural experts unanimously appeal, administer environment, must reduce fertilizer amount.Expert, the new academician of the Chen Wen of China Agricultural University think, the excessive chemical fertilizer of using of China has arrived the limit at present.With the average amount of application of nitrogenous fertilizer Wei Li， China, be nearly 3 times, more than 8 times of Australia of the U.S., a series of serious problems such as this has caused, and Soil structure is destroyed, nature biotechnology diversity and ecosystem stability reduction.Agricultural microbiology Professional Committee of Chinese Society for Microbiology director doctor Li Jun points out, wideling popularize microorganism fertilizer, to coordinate chemical fertilizer application be exactly one of solution to this problem.
Microorganism fertilizer is a kind ofly with microbial life activity and product thereof, to cause farm crop to obtain the microorganism live body goods of specific fertilizer effect, it at culture fertility, improve chemical fertilizer utilization ratio, suppress farm crop there is to irreplaceable effect the aspects such as utilizations of becoming thoroughly decomposed of the absorption of the objectionable impuritiess such as heavy metal and agricultural chemicals, purification and rehabilitating soil, promotion agricultural crop straw and municipal wastes, raising quality of agricultural product.The efficacy exertion of microbial fertilizer is mainly by the synergism to traditional fertilizer, fertilizer, improvement activation to soil, and the modes such as physiological action of microorganism realize.Therefore, microbial fertilizer has just had multiple fertility and the effect of chemical fertilizer, fertilizer, beneficial organism bacterium, is activated fertilizer nutrient, improves effect of fertilizer, Crop Improvement quality, promotes the desirable fertilizer of agricultural produce synergy.Microbial fertilizer is live body fertilizer, and a large amount of beneficial microorganism vital movements that its effect mainly contains by it complete.Only have these beneficial microorganism vital movements of working as to complete.Only have when these beneficial microorganisms are in vigorous breeding and metabolic situation, Substance Transformation and useful meta-bolites could constantly form.Therefore, beneficial microorganism kind in microbial fertilizer, whether vital movement is vigorous is the basis of its validity, and is to take the form of the principal elements such as nitrogen, phosphorus, potassium and how many for basic unlike other fertilizer.Just because of microbial fertilizer is the preparation of living, so its fertilizer efficiency and number of viable, intensity and ambient environmental conditions are closely related, comprise temperature, moisture, potential of hydrogen, nutritional condition and formerly live in indigenous microorganism repulsive interaction in soil and have certain influence.
China's microbial fertilizer industry is compared with other countries, have that Varieties is many, the feature of applied range, especially, aspect the product innovation being composited at research and development and application microorganism and organic nutrient substance, microorganism and inorganic nutrient substance, maintain the leading position.Yet, because China lack to drop in research aspect microbial fertilizer for a long time, make that the microbial fertilizer industry of China still exists that integral level is not high, technological innovation is not enough, quality product and effect show understable problem.At agricultural sustainable development, become today of human consensus, these problems have become microorganism fertilizer industry letter " bottleneck " to be got through.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, and a kind of composite fertilizer is provided:
Parts by weight consist of: yeast microbial inoculum 5-10 part, colloid bud pole bacteria agent 5-10 part, phosphorus decomposing Bacillus megaterium microbial inoculum 5-10 part, subtilis culture 10-15, Aspergillus awamori culture 1-5, plant lactobacillus agent 1-4 part.
Described subtilis culture preparation: cultivate subtilis from inclined-plane switching, the seed liquor after spreading cultivation is step by step transferred in fermentor tank, the complete fermented liquid that ferments obtains subtilis culture through Plate Filtration, after dry.
The preparation method of plant lactobacillus agent: from inclined-plane switching culturing plants Bacterium lacticum, the seed liquor after spreading cultivation is step by step transferred in fermentor tank, and the complete fermented liquid that ferments is concentrated in vacuo to 45% of original volume through low-temperature negative-pressure, obtains bacterium concentrated solution.Add carrier: in concentrated solution, add the carrier mixing, mix; The weight ratio of concentrated solution and carrier is 0.5-0.6:1, and vehicle group becomes: CaCO
320-30 part, dextrin 10-15 part.Fluidised bed drying, 50 ℃ of drying temperatures.
Aspergillus awamori culture preparation: spawn culture, solid fermentation is cultivated: spore liquid is inoculated in aspergillus oryzae solid state fermentation culture material, and 26-33 ℃ is cultured to mycelia and covers with culture material, and low temperature fluidized-bed dry, pulverize dry thing;
This product using method is simple, every mu of ground usage quantity 0.3-1 kilogram, and cost is only 80-100 unit/mu, spray on seed dressing or the front earth's surface of pouring water vegetative period.
Beneficial effect
Through experimental results show that for many years, the conversion energy of soil organisms enzyme improves soil compaction, acidifying, the saliferous problem partially causing to fertilize because of long-term, sandy soil reunion, clay are loosened, strengthen water conservation, fertilizer-preserving ability, in soil, a large amount of biological enzymes has the ability of minute phosphorus decomposing.But the inorganic fertilizer that life-time service is a large amount of and agricultural chemicals, disappear some probiotics extinction.So we are manured into soil with appropriate compound bio-enzyme, can impel the loosing soil of active layer, increase soil fertility, build a good soil environment that is suitable for crop root growth.The conversion of soil organisms enzyme, reducing agriculture production cost, reducing use, the recovery soil ecology soil fertility of chemical fertilizer and effectively improve the aspects such as crop yield and all can play significant effect, gives full play to the organic usefulness of soil.
Colloid bacillus cereus claims again silicate bacteria, its key property is potassium, the silicon that can decomposite in the mineral such as feldspar, mica, also can decomposite the phosphorus in phosphatic rock, and secretion plant growth substance and plurality of enzymes, to strengthen the resistibility of crop to some diseases.
Endobacillary potassium, after thalline death, dissociates out, can be absorbed and used by plants again.As a kind of critical function bacterium in microbial fertilizer, can improve Soil Available potassium, phosphorus content, improve the multiple effects such as crop yield and quality.
Rui Gu bio tech ltd, Baoding provides colloid bacillus cereus bacterium powder;
Fertilizer of the present invention can strengthen the drought-resistant ability of crop.Colloid bud pole bacterium in composite bacteria of the present invention can secrete the growth-promoting substances such as growth hormone material and Plant hormones regulators,gibberellins material, extraneous root strong sprout; Decompose element silicon in soil and, for plant utilization, the wax layer of plant is thickened, improve plant water keeping ability; Can promote soil aggregate to form, keep soil from packing together; Spoiled soil capillarity, prevents soil water evaporation.
Fertilizer of the present invention is nutritious, and organic content is high.Can meet the need of production of Organic food.
Fertilizer of the present invention is formed by compound microorganism ferments, can improve the micro-ecological environment of soil, for the growths of farm crop provides beneficial conditions.
Fertilizer of the present invention can strengthen the diseases and insect pests resistance of crop.
Fertilizer of the present invention can be improved soil.In fertilizer, beneficial microorganism can produce glucide, account for 0.1% of the soil organism, with plant mucilage, mineral idiosome and organic colloid combine, can improve soil aggregate, strengthen the physicals of soil and the loss of minimizing soil particle, under certain conditions, can also participate in soil ulmin and form.So use microbial fertilizer, can improve soil physical property, be conducive to increase soil fertility.
This product using method is simple, every mu of ground usage quantity 0.3-1 kilogram, and cost is only 80-100 unit/mu, spray on seed dressing or the front earth's surface of pouring water vegetative period.
Embodiment
Unless stated otherwise, in the present invention, technique means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope, the various changes that the reaction conditions in these embodiments, separation and Extraction condition are carried out or change also belong to protection scope of the present invention.
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Phosphorus decomposing Bacillus megaterium is a kind in Bacillus (Bacillus).Motion, forms pod membrane, aerobic.From glucose, produce acid, also often from pectinose and N.F,USP MANNITOL, produce acid.Hydrolyzed starch, does not produce lecithinase, and VP is negative.The organophosphorus that can decompose in soil becomes the available rapid available phosphorus of plant.The agent of phosphorus decomposing bacillus megaterium is provided by Cangzhou prosperous generation thing technical institute, address: liberation West Road, Canal Zone, Hebei China Cangzhou City chin or cheek and Building A, international business affairs center 1 district 807-812.
Rui Gu bio tech ltd, Baoding provides colloid bacillus cereus bacterium powder.
Subtilis provided by the invention (Bacillus subtilis) Li-2013-02.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013, and (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, BeiJing, China city institute), preserving number is CGMCC No.7926.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is oyster white, and surface drying is opaque, and neat in edge, for having the aerobic bacteria of mobility.Microscopy is elongated rod shape, and gramstaining is positive.This bacterium can utilize Citrate trianion, and nitrate reductase, V-P test into positive.
Described subtilis (Bacillus subtilis) Li-2013-02 is preserved in the high temperature resistant α-amylase of product in this laboratory subtilis Li-2013 by a strain obtains through UV-LiCl-ethyl sulfate Mutation screening, specifically screens step as follows:
(1) preparation of bacteria suspension
By in the mono-bacterium colony of the Li-2013 growing after plate streaking separation access seed culture medium, 100r/min, cultivates after 12h for 40 ℃, uses physiological saline washed twice after getting 1mL medium centrifugal, and in resuspended and 9mL physiological saline.
(2) UV-LiCl-ethyl sulfate complex mutation
Bacteria suspension is placed in to aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the ultraviolet lamp of power 15w.To after gradient dilution, coat lithium chloride flat board through the bacterium liquid irradiating, and contrast with the bacterium liquid dilution painting flat board without uv irradiating.Above-mentioned coating is dull and stereotyped uniformly, with cloth or the newspaper of black, wrap, put 40 ℃ and cultivate 48h, on the flat board of bacterium colony, filter out hydrolysis circle and choose to inclined-plane and preserve with colony diameter ratio the maximum growing, after purifying, be mixed with bacteria suspension, after gradient dilution, fully mix with ethyl sulfate stoste, and process 40min in 40 ℃ of concussions, the bacterium liquid of processing is coated to lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, put 40 ℃ and cultivate 48h, go out hydrolysis circle and choose to inclined-plane and preserve with colony diameter ratio the greater growing on the flat board of bacterium colony primary dcreening operation, after purifying, obtain three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 that obtain, Li-2013-02, Li-2013-03 carries out shake flask fermentation in the 250mL shaking flask that contains 30mL fermention medium, seed inoculum size 10% (V/V), 40 ℃, 100r/min are cultivated 72h, and centrifuging and taking fermented supernatant fluid makes crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, under 105 ℃, pH 4.2 conditions, 1min liquefaction 1mg Zulkovsky starch, is 1 enzyme activity unit, represents with U/mL.
After measured, bacterial strain Li-2013-02, is stable superior strain, and enzyme work reaches 30000U/mL, than original strain enzyme, lives and improves 1.6 times.
Described lithium chloride is dull and stereotyped: starch 1%, and peptone 1%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: yeast powder 0.5%, peptone 1%, Zulkovsky starch 1%, NaCl 1%.
Described fermention medium: Semen Maydis powder 5%～15%, soybean cake powder 4%～10%, (NH) 2SO40.4%, K2HPO40.8%, CaCl20.2%.
Described shake-flask culture condition: this bacterium in the 250mL shaking flask that contains 30mL fermention medium, inoculum size 10% (V/V), 100r/min, 40 ℃ of fermentation culture 72h.
By bacterial strain Li-2013-02 fermentation, obtained a kind of high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme thermal adaptation a wider range, optimum temperature is between 105-115 ℃, and better 110 ℃ of following temperature stabilities of preserving, and 115 ℃ above to preserve long-time temperature stabilities poor.
(2) this enzyme optimal reaction pH value is 4.2.Between pH value 3.0-7.0, all having high enzyme vigor, is 3.0 o'clock enzyme complete stabilities alive in pH value.
(3) enzymic activity: by mutant strain Li-2013-02 provided by the present invention, the high temperature resistant α-amylase enzyme activity of preparation is 30000-35000U/ml.
1, the present invention uses the method for UV-LiCl-ethyl sulfate complex mutation to obtain the subtilis Li-2013-02 of a strain high-yield thermostable α-amylase, and this bacterial strain has acidproof, thermotolerance by force, produces the high feature of enzyme activity.
2, there is the high temperature resistant α-amylase enzyme activity of this bacterial strain production gained up to 30000-35000u/ml; Applicable temperature scope is 25-115 ℃, and 110 ℃ of optimal reactive temperatures, at 110 ℃ of enzymes complete stability alive; Applicable pH value in reaction scope is 3.0-7.0, in pH value, be 3.0 o'clock enzyme complete stabilities alive, optimal reaction pH value is 4.2, higher than existing high temperature resistant α-amylase enzyme activity, enzyme effect optimum pH wide scope, heatproof degree is high, is particularly suitable for that temperature of reaction is high, liquefaction process and Mashing process the industrialization demand of depositing.
Embodiment 1
A composite fertilizer, parts by weight consist of: 8 parts of yeast microbial inoculums, 8 parts of colloid bud pole bacteria agents, 8 parts of phosphorus decomposing Bacillus megaterium microbial inoculums, subtilis culture 12, Aspergillus awamori culture 3,3 parts of plant lactobacillus agent.
The bacterial classification adopting is as follows:
Subtilis (Bacillus subtilis subsp) CGMCC7926
Plant lactobacillus (Lactobacillus plantarum) CICC20764
Aspergillus awamori (Aspergillus awamori) CGMCC3.6484
S. cervisiae is selected cicc1526, cicc1413, and bacterial classification is all purchased from Chinese industrial microbial strains preservation center.
Referring to Xiao Dongguang work, " production of Active Dry Yeast and the utilisation technology ”， Inner Mongol People's Press publish the production method of yeast microbial inoculum for 1994.
The preparation method of Aspergillus awamori microbial inoculum:
Technical scheme is as follows:
Slant strains activation culture: Aspergillus awamori slant strains is transferred on slant medium, cultivates 3 days for 27 ℃.
Solid first order seed is cultivated: picking Aspergillus awamori slant strains accesses in 500 milliliters of triangular flasks that 100 grams of substratum are housed carries out seed culture, cultivates 3 days for 30 ℃.
Solid secondary seed is cultivated: above-mentioned cultured solid first order seed is stirred as adding after fragment and in 5000 milliliters of triangular flasks that 1000 grams of substratum are housed, carries out seed culture, culture condition: cultivate 3 days for 30 ℃.
Solid fermentation is cultivated: secondary shake-flask seed is pulverized, added to be equipped with in the fermentation vat of sterilising medium or pallet to mix rear cultivation, bent material culture temperature is controlled at 26-35 ℃, humidity 80-90%, every 10 hours stirrings once, incubation time 5-7 days; The cultivation of solid koji material adopts conventional bent material culture technique; Treat that culture material covers with mycelia and can finish to cultivate, substratum is in advance through high temperature steaming sterilising treatment, and sterilising conditions is controlled 121 ℃ of temperature, 1 hour time.
Drying and crushing: fermentation ends culture material is dried on fluidized-bed or other drying plants, and drying temperature is controlled at 60 ℃, is dried to moisture content below 10%, then solid culture medium is pulverized, and crushing material aperture is more than 60 orders.
Substratum forms: solid material: wheat bran 80%, and soybean cake powder 10%, W-Gum 10%, adds equivalent tap water; Initial pH nature.
The preparation method of subtilis culture:
1. the acquisition of fermented liquid: adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: subtilis slant strains is accessed in 500 ml shake flasks to 100 milliliters of substratum loading amounts, 180 revs/min of rotary shaking tables, 30 ℃ of culture temperature, incubation time 24 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum size access of 10%, culture condition is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of substratum loading amounts, 100 revs/min of rotary shaking tables, 30 ℃ of culture temperature, incubation time 24 hours with 10% inoculum size;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 10% inoculum size access cubic capacity is 150L, fermention medium loading amount 100L, 28 ℃ of culture temperature, 100 revs/min of stirring velocitys, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours;
(5) fermentation culture: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification be take to 10% inoculum size access cubic capacity, 1 ton of fermention medium loading amount, 28 ℃ of culture condition culture temperature, 100 revs/min of stirring velocitys, ventilation (V/V) 1:0.5, tank pressure 0.05Mpa, incubation time 24 hours.
Substratum forms: glucose 6%, and yeast extract 1%, peptone 0.2%, CaCO3 1%, pH6.8.
The preparation method of plant lactobacillus agent:
(1) first order seed is cultivated: plant lactobacillus bacterial classification is accessed in 500 ml shake flasks to 100 milliliters of substratum loading amounts, 30 ℃ of culture temperature, incubation time 24 hours;
(2) secondary seed is cultivated: by first order seed, according in 500 milliliters of secondary seed shaking flasks of inoculum size access of 10%, culture condition is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed shaking flasks to 1000 milliliters of substratum loading amounts, 30 ℃ of culture temperature, incubation time 24 hours with 10% inoculum size;
(4) first class seed pot is cultivated: three grades of seeds be take to the first class seed pot that 5% inoculum size access cubic capacity is 150L, fermention medium loading amount 100L, 30 ℃ of culture temperature, tank pressure 0.05Mpa, incubation time 18 hours;
(5) fermentor cultivation: it is 3 tons of secondary seed tanks that first class seed pot bacterial classification be take to 5% inoculum size access cubic capacity, 2 tons of fermention medium loading amounts, 30 ℃ of culture condition culture temperature, tank pressure 0.05Mpa, incubation time 22 hours.The complete fermented liquid that ferments is concentrated in vacuo to 45% of original volume through low-temperature negative-pressure, obtains bacterium concentrated solution.Add carrier: in concentrated solution, add the carrier mixing, mix; The weight ratio of concentrated solution and carrier is 0.5:1, and vehicle group becomes: CaCO
325 parts, 12 parts, dextrin.Fluidised bed drying, 50 ℃ of drying temperatures.
Substratum consists of: casein peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, sodium acetate 0.5%, citric acid diamines 0.2%, Tween 80 0.1%, K2HPO4 0.2%, MgSO4.7H2O0.02%, MnSO4.H2O 0.005%, and CaCO3 2%, agar 1.5%, pH6.8.
Embodiment 2
Substantially with embodiment 1
A composite fertilizer, parts by weight consist of: 9 parts of yeast microbial inoculums, 7 parts of colloid bud pole bacteria agents, 7 parts of phosphorus decomposing Bacillus megaterium microbial inoculums, subtilis culture 13, Aspergillus awamori culture 2,2 parts of plant lactobacillus agent.
The experiment of product effect
Selection experimental field and test design: tested 27 days-September 30 April in 2009 and carry out in Ba Bao village, dapple pond town, Yanchi county Ningxia.
Experimental plot reaches 10 mu of field maize plantings, uses 0.5 kilogram every mu of product of the present invention respectively when plantation, emerges and by loose ground mode, uses 0.5 kilogram of invention product about 1 month, and control group is used conventional fertilizer.
Invention product is used corn field corn yield to reach 650 kilograms, and control group reaches 500 kilograms; The use of invention product makes corn per mu yield improve 30%.This plot was at the 2nd year plant spring wheat, and spring wheat production has reached 400 kilograms, than control group per unit area yield, has improved 20%.And experimental plot Soil structure is good, without bulk and hardening.
Claims (5)
1.Yi Zhong composite fertilizer, parts by weight consist of: yeast microbial inoculum 5-10 part, colloid bud pole bacteria agent 5-10 part, phosphorus decomposing Bacillus megaterium microbial inoculum 5-10 part, subtilis culture 10-15, Aspergillus awamori culture 1-5, plant lactobacillus agent 1-4 part.
2. a kind of composite fertilizer according to claim 1, described subtilis culture preparation: cultivate subtilis from inclined-plane switching, seed liquor after spreading cultivation is step by step transferred in fermentor tank, and the complete fermented liquid that ferments obtains subtilis culture through Plate Filtration, after dry.
3. a kind of composite fertilizer according to claim 1, described subtilis (Bacillus subtilis) preserving number is CGMCCNo.7926.
4. a kind of composite fertilizer according to claim 1, parts by weight consist of: 8 parts of yeast microbial inoculums, 8 parts of colloid bud pole bacteria agents, 8 parts of phosphorus decomposing Bacillus megaterium microbial inoculums, subtilis culture 12, Aspergillus awamori culture 3,3 parts of plant lactobacillus agent.
5. a kind of composite fertilizer according to claim 1, parts by weight consist of: 9 parts of yeast microbial inoculums, 7 parts of colloid bud pole bacteria agents, 7 parts of phosphorus decomposing Bacillus megaterium microbial inoculums, subtilis culture 13, Aspergillus awamori culture 2,2 parts of plant lactobacillus agent.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104341239A (en) * | 2014-09-22 | 2015-02-11 | 北京科慧通智慧科技有限公司 | Biofertilizer |
| CN104761373A (en) * | 2015-03-31 | 2015-07-08 | 安徽中农化工国际贸易有限公司 | Organic bio-bacterial manure and preparation method thereof |
| CN105234168A (en) * | 2015-11-20 | 2016-01-13 | 福建康德贝生物科技有限公司 | Application of bacillus subtilis |
| CN110218683A (en) * | 2019-06-27 | 2019-09-10 | 中国科学院寒区旱区环境与工程研究所 | Microbial composite, microorganism formulation and method for husky area's soil improvement |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100190844A1 (en) * | 2009-01-23 | 2010-07-29 | National Taiwan University | Process for preparation of aglucone isoflavones |
| CN101914447A (en) * | 2010-07-31 | 2010-12-15 | 大连三科生物工程有限公司 | Complex microbial inoculum 707 and preparation method and application thereof |
| CN102153382A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial foliar fertilizer spraying agent and using method thereof |
| CN102153380A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Method for preparing composite probiotic spraying agent |
| CN102277343A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Biological composite enzyme |
| CN102277349A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Production method of biological compound enzyme |
-
2013
- 2013-11-20 CN CN201310589442.XA patent/CN103626531B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100190844A1 (en) * | 2009-01-23 | 2010-07-29 | National Taiwan University | Process for preparation of aglucone isoflavones |
| CN101914447A (en) * | 2010-07-31 | 2010-12-15 | 大连三科生物工程有限公司 | Complex microbial inoculum 707 and preparation method and application thereof |
| CN102153382A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Composite microbial foliar fertilizer spraying agent and using method thereof |
| CN102153380A (en) * | 2010-12-15 | 2011-08-17 | 沈阳科丰牧业科技有限公司 | Method for preparing composite probiotic spraying agent |
| CN102277343A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Biological composite enzyme |
| CN102277349A (en) * | 2011-07-24 | 2011-12-14 | 李绩 | Production method of biological compound enzyme |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104341239A (en) * | 2014-09-22 | 2015-02-11 | 北京科慧通智慧科技有限公司 | Biofertilizer |
| CN104761373A (en) * | 2015-03-31 | 2015-07-08 | 安徽中农化工国际贸易有限公司 | Organic bio-bacterial manure and preparation method thereof |
| CN105234168A (en) * | 2015-11-20 | 2016-01-13 | 福建康德贝生物科技有限公司 | Application of bacillus subtilis |
| CN110218683A (en) * | 2019-06-27 | 2019-09-10 | 中国科学院寒区旱区环境与工程研究所 | Microbial composite, microorganism formulation and method for husky area's soil improvement |
| CN110218683B (en) * | 2019-06-27 | 2020-06-26 | 中国科学院西北生态环境资源研究院 | Microbial composition, microbial preparation and method for improving soil in sand area |
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