CN103616376A - Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products - Google Patents
Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products Download PDFInfo
- Publication number
- CN103616376A CN103616376A CN201310569766.7A CN201310569766A CN103616376A CN 103616376 A CN103616376 A CN 103616376A CN 201310569766 A CN201310569766 A CN 201310569766A CN 103616376 A CN103616376 A CN 103616376A
- Authority
- CN
- China
- Prior art keywords
- solution
- steroidal anti
- test
- chinese patent
- inflammatory drugs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 0 *C1=CC=CCC1 Chemical compound *C1=CC=CCC1 0.000 description 2
Abstract
The invention discloses a method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products and a kit based on the method, and aims to provide a quick test method which is exclusive, sensitive, simple and convenient and strong in field operability. The method comprises the following steps: (1) placing 1 part of a test sampler in a test tube with a stopper, adding 3-4ml of an organic solvent for extraction, and shaking for 1 minute to obtain a test sampler crude extract; (2) filtering out two parts of filtrate by an organic microfiltration membrane, wherein each part of filtrate is about 1ml and the two parts of filtrate are separately placed in two colorless glass containers; (3) adding about 0.2ml of a tetrazole reagent into one of the two parts of filtrate to obtain a to-be-tested solution, taking the other part of filtrate as a control solution, and sealing and uniformly mixing the two solutions; (4) after placing the two solutions for 2-3 minutes, carrying out colorimetric observation within 3 minutes; and (5) judging the result: if the to-be-tested solution is of a certain color and is darker than the color of the control solution, judging the solution to be positive, on the contrary, negative.
Description
Technical field
The present invention discloses a kind of detection method, and specifically a kind of method of utilizing chemical reagent to detect steroidal anti-inflammatory drugs in Chinese patent drug, health food, belongs to medical detection technique field.
Background technology
Glucocorticoid (glucocorticoid, GCS) be a class steroid hormone of zona fasciculata secretion in adrenal cortex, be mainly cortisol (cortisol), have and regulate sugar, fat and the biosynthesizing of protein and the effect of metabolism, also have that Immunosuppression is replied, an anti-inflammatory, antitoxin, Antishock function.Being called " glucocorticoid " is because it regulates the activity of carbohydate metabolism to be familiar with by people the earliest, and in clinical position, cortin is widely used in bronchial astehma and the treatment of inflammation severe because having compared with strong anti-inflammatory anti-allergy action.
In the conventional therapy of bronchial astehma, when the symptomatic treatments such as microbiotic is applied, bronchodilator is used, relieving cough and reducing sputum can not reach satisfactory effect, when without absolute contraindication, apply cortin and carry out impact treatment.Rheumatism and rheumatoid arthritis are common disease, are osteoarticular a kind of chronic inflammation and destructive disease, belong to stubborn disease, and needs of patients Long-term taking medicine is to control the state of an illness.Drug therapy mainly comprises non-steroid anti-inflammatory drug (NSAIDs), cortex hormone of aadrenaline and improves the antirheumatic of the state of an illness.And cortin is mainly used in severe treatment, also low dose of short is advisable in principle.
In health food, Chinese medicine, the case of illegal interpolation chemicals is of common occurrence in recent years.Traditional Chinese medicine has the history of several thousand in China, and, security advanced the be subject to common people little with toxic and side effect trust, and lawless person seeks exorbitant profit whereby, in health food, Chinese medicine, adds chemicals, to reach the object of short-term response.Illegal health food, the Chinese patent drug adding of long-term taking, endanger huge, more than delay treatment, and spinoff is obvious, especially adrenocortical hormone, even if this medicine that all needs careful use under western medical treatment, has been found in the case that has illegal interpolation in relieving cough and asthma class and antirheumatic health food, Chinese patent drug.Therefore to corticoid medicine, illegal interpolation should strengthen strike and supervision.
Corticoid drug molecule has steroidal structure, corresponding with non-steroid anti-inflammatory drugs such as aspirin, becomes steroidal anti-inflammatory drugs; C
17α-ol ketone group on position has reductibility, represents that medicine has prednisone acetate, metacortandracin, hydrocortisone acetate, cortisone, hydrocortisone, dexamethasone acetate, dexamethasone etc.Steroid corticosteroid drug, there is the effect of good anti-inflammatory, also have the curative effect of antiallergic action concurrently, but use this class adverse drug reaction obvious, mainly contain lipid-metabolism and water and salt metabolic disturbance, show as moon shaped face, the serious adverse reactions such as concentric hypertrophy, hypertension, osteoporosis, even cause femur necrosis.If not this class medicine method is entrained in wines used as antirheumatic, relieving cough and asthma class Chinese patent drug and health food, very easily causes serious adverse reaction, causes serious harm to extensive patients.
Applicant hits in undeclared prescription drugs activity and finds in special project, the illegal steroidal anti-inflammatory drugs that added in part wines used as antirheumatic Chinese patent drug and health food.The illegal activities of corticoid chemicals are added without authorization in investigation in wines used as antirheumatic class and relieving cough and asthma class Chinese patent drug and health food, are long-term tasks, and coverage is wide, and check workload is large.
At present, in check Chinese patent drug and health food, the method for steroidal anti-inflammatory drugs doping mainly contains high performance liquid chromatography, Liquid Chromatography-Mass Spectrometry.High performance liquid chromatography and Liquid Chromatography-Mass Spectrometry are highly sensitive, and result accurately and reliably, but is not suitable for the field test that mobility is large, and in vast rural area and small and medium-sized cities underdeveloped, carrying out check activity has certain difficulty.
Summary of the invention
For the problems referred to above, the present invention aims to provide a kind of shortcoming that prior art exists that overcomes, analysis cost is low, do not need to use valuable analytical instrument, specificity is strong, highly sensitive, phenomenon is easy to observe, and is applicable to whether the adulterate method of inspection of steroidal anti-inflammatory drugs of field test Chinese patent drug, health food.
The second object of the present invention is to provide said method in the application of preparing steroidal anti-inflammatory drugs quick testing reagent kit.
The application whether the 3rd object of the present invention is to provide said method to adulterate in the examination of steroidal anti-inflammatory drugs at check wines used as antirheumatic class and relieving cough and asthma class Chinese patent drug, health food.
The first technical scheme of the present invention is the screening method of steroidal anti-inflammatory drugs in such a kind of Chinese patent drug, health food, by following steps, formed successively: (1) is got 1 deal test sample and is placed in tool plug test tube, add and extract organic solvent 3 ~ 4ml, jolting 1min, obtains test sample crude extract; (2) use organic system miillpore filter, filter out 2 parts of filtrates, each about 1ml, is placed in respectively 2 flint glass containers; (3) in a filtrate, add about 0.2ml tetrazole reagent as test solution wherein, another part is solution in contrast, and 2 parts of solution sealings are mixed; (4) 2 parts of solution are placed after 2 ~ 3min, in 3min, carried out colorimetric observation; (5) result judgement: if test solution is aobvious red and darker than contrast solution colour, be judged to be the positive, on the contrary negative.
1 described deal test sample is the test sample of a dose.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, described capsule and soft capsule are got content and be placed in tool plug test tube, the first porphyrize of tablet and pill, then be placed in tool plug test tube.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, described Chinese patent drug, health food comprise claims to have wines used as antirheumatic, relieving cough and asthma etc. may add Chinese patent drug, the health food that cortin is steroidal anti-inflammatory drugs.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, described steroidal anti-inflammatory drugs has C
17-α-ol ketone group, has reducing property and reacts with tetrazole test solution, comprises prednisone acetate, metacortandracin, hydrocortisone acetate, cortisone, hydrocortisone, dexamethasone acetate, dexamethasone etc.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory medicine, is characterized in that, the extraction organic solvent described in step (1) is alkaline ethanol solution; Alkalescence can be provided by inorganic bases such as NaOH, potassium hydroxide, also can be provided by organic bases such as tetrabutylammonium; In order to dissolve alkaline matter, can adopt absolute methanol to prepare alkaline strong solution, then mix with proper proportion with absolute ethyl alcohol; Ethanol used should be the pure absolute ethyl alcohol of top grade or ethanol,aldehyde free; Final solution pH preferably reaches more than 13, and proportion of ethanol is high, moisture is below 5%, and chromogenic reaction is the sensitiveest, rapid.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, extracts the mixed solvent that organic solvent is alkali lye and ethanol described in step (1); Part test sample is first used alcohol extract, then can develop the color while adding alkali lye, produces false positive results; First mix and can avoid such false positive.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, in step (2), test sample crude extract is filtered out to 2 parts, a for colour developing, a for controlled observation.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, the described tetrazole reagent of step (3) comprises TTC (TTC, 2,3,5-triphen tetrazole, its colour developing product is red) and blue tetrazolium (BT, 3,3 '-, dimethoxy phenyl-bis--4,4 '-(3,5-diphenyl) tetrazolium chloride, when it is excessive, product is skipper).Above-mentioned 2 kinds of tetrazole reagent can be mixed with 0.2% ~ 0.5% chromophoric solution with absolute ethyl alcohol (top grade is pure), and are kept at (lucifuge) in brown reagent bottle.
Above-mentioned tetrazole reagent, is characterized in that, preferentially uses TTC; Blue tetrazolium is highly sensitive but easily produce false positive.
In above-mentioned Chinese patent drug, health food, the screening method of steroidal anti-inflammatory drugs, is characterized in that, step (4) standing time is that 2 ~ 3min question response is complete, and at 3min later with interior observation; After producing false positive for a long time, after under illumination or air exposure, colour developing can disappearance generation false negative for a long time.
Take common prednisone acetate, dexamethasone acetate, dexamethasone, hydrocortisone, hydrocortisone acetate is that detection sensitivity is investigated in representative, it is very obvious that the developing sensitivity of this method all can reach 0.20mg(colour developing) below, and the clinical ampoule of prednisone acetate, dexamethasone acetate, dexamethasone, hydrocortisone and hydrocortisone acetate is about 5 ~ 10mg successively, 0.75 ~ 3mg, 0.75 ~ 3mg, 20mg and 20mg.Therefore the enough assurances of developing sensitivity of the present invention can undetected Chinese patent drug, the steroidal anti-inflammatory drugs in health food.
The second technical scheme of the present invention is the kit that the screening method of steroidal anti-inflammatory drugs in such a kind of Chinese patent drug, health food is used, in box body, be provided with at least one and fill the tool plug test tube that 4ml extracts solvent, at least one fills the brown reagent bottle of 1ml tetrazole reagent, at least two water white reaction bulbs, the disposable syringe of at least one 5ml, and organic system miillpore filter.
Test principle of the present invention is as follows:
Steroidal anti-inflammatory drugs, i.e. corticoid medicine, C in molecular structure
17α-ol ketone group on position has reductibility, can react colour generation with tetrazole test solution.Tetrazolium has oxidisability, and C
17after the reaction of-α-ol ketone group, be reduced and develop the color.
Conventional tetrazolium has two kinds:
(1) TTC: 2,3,5-triphen tetrazole (2,3,5-triphenyltetrazolium chloride, is abbreviated as TTC), its reduzate is water-fast peony San Ben formazan,
λmax, at 480 ~ 490nm, also claims red tetrazolium (red tetrazoline);
(2) blue tetrazolium (blue tetrazolium, BT): 3,3 '-dimethoxy phenyl-bis--4,4 '-(3,5-diphenyl) tetrazolium chloride (3,3 '-dianisole-bis[4,4 '-(3,5-dipheny) tetrazolium chlorid]), its reduzate is lazuline Shuan formazan
λmax is near 525nm.Structural formula and the reaction principle of TTC and BT are as follows:
Because Chinese patent drug and health food extract often have the different color of depth degree, thus test sample crude extract is filtered out to 2 parts, a for colour developing, a for controlled observation.
Compared with prior art, the present invention has following beneficial effect:
Analysis cost is low, do not need Special Equipment, specificity is strong, highly sensitive, be applicable to the method for quickly detecting whether field test wines used as antirheumatic class and relieving cough and asthma class Chinese patent drug, health food mix steroidal anti-inflammatory drugs, meet the needs of wines used as antirheumatic class and relieving cough and asthma class Chinese patent drug, health food supervision and inspection, simultaneously for the specialized laboratories of checking wines used as antirheumatic class and relieving cough and asthma class Chinese patent drug, health food whether to mix steroidal anti-inflammatory drugs provides quick screening method, to reach the object that reduces analysis cost, raising checkability.Because experimental phenomena of the present invention appears at after colour developing 2 ~ 3min, check conclusion accuracy is high, easy and simple to handle quick, does not need to use the advantages such as expensive instrument.
Steroidal anti-inflammatory drugs fast screening reagent kit in Chinese patent drug manufactured according to the present invention, health food, can meet the needs that relevant food and medicine supervision and inspection is carried out in vast rural area.
Embodiment
experimental technique
1. the preparation of test liquid
Get the test sample of a dose, capsule and soft capsule are got content, and the first porphyrize of tablet and pill or shred is placed in tool plug test tube, adds and extracts organic solvent 3 ~ 4ml, and jolting 1min, obtains test sample crude extract; With organic system miillpore filter, filter test sample crude extract and obtain 2 parts for each about 1ml of examination filtrate.
2. chromogenic reaction
In a filtrate, add wherein the about 0.2ml of tetrazole reagent, sealing, jolting is for several times.
3. result
2 parts of solution are placed after 2 ~ 3min, in 3min, carried out colorimetric observation; (5) result judgement: if test solution is aobvious red and darker than contrast solution colour, be judged to be the positive, on the contrary negative.After colour developing, placed and likely produced for a long time false positive, after colour developing can disappearance generation false negative under illumination or air exposure for a long time.
embodiment 1
Efficient Fengshiling Capsules (capsule, indicating dose is each 2 ~ 3, High Performance Liquid Chromatography/Mass Spectrometry method measurement result detects containing prednisone acetate.）
Get 2 capsules contents, join in the tool plug test tube that fills 4ml extraction organic solvent (10% potassium hydroxide methanol solution-absolute ethyl alcohol (10:90)), jolting 1min, with the disposable syringe of 5ml, draw about 3ml, with organic system miillpore filter, filter, filter 2 parts of each about 1ml, put in 2 colourless transparent glass bottles, portion adds about 0.2ml developer (0.2% TTC ethanol solution) as test solution wherein, the solution in contrast that does not add developer, 2 parts of solution sealings are mixed to observe phenomena after placement 3min.Result contrast solution is faint yellow, and test solution is pale red, darker than contrast liquid color, is positive, and shows that sample contains steroidal anti-inflammatory drugs, and this result confirms that with mass spectrum result is consistent.
embodiment 2
Arthritis capsule (capsule, indicating dose is each 2 ~ 3, High Performance Liquid Chromatography/Mass Spectrometry method measurement result detects prednisone acetate.）
Get 2 capsules contents, join in the tool plug test tube that fills 4ml extraction organic solvent (10% potassium hydroxide methanol solution-absolute ethyl alcohol (10:90)), jolting 1min, with the disposable syringe of 5ml, draw about 3ml, with organic system miillpore filter, filter, filter 2 parts of each about 1ml, put in 2 colourless transparent glass bottles, portion adds about 0.2ml developer (0.2% TTC ethanol solution) as test solution wherein, the solution in contrast that does not add developer, 2 parts of solution sealings are mixed to observe phenomena after placement 3min.Result contrast solution is pale red, and test solution takes on a red color, darker than contrast liquid color, is positive, and shows that sample contains steroidal anti-inflammatory drugs, and this result confirms that with mass spectrum result is consistent.
embodiment 3
Yunnan Baiyao Capsule (capsule, indicating dose is each 2 ~ 3, High Performance Liquid Chromatography/Mass Spectrometry method measurement result does not detect containing steroidal anti-inflammatory drugs.）
Get 2 capsules contents, join in the tool plug test tube that fills 4ml extraction organic solvent (10% potassium hydroxide methanol solution-absolute ethyl alcohol (10:90)), jolting 1min, with the disposable syringe of 5ml, draw about 3ml, with organic system miillpore filter, filter, filter 2 parts of each about 1ml, put in 2 colourless transparent glass bottles, portion adds about 0.2ml developer (0.2% TTC ethanol solution) as test solution wherein, the solution in contrast that does not add developer, 2 parts of solution sealings are mixed to observe phenomena after placement 3min.Result contrast solution is water white transparency, and test solution water white transparency is dark unlike contrast liquid color, is negative, and shows that sample does not contain steroidal anti-inflammatory drugs, and this result confirms that with mass spectrum result is consistent.
embodiment 4
Eight treasures (choice ingredients of certain special dishes) jet pellet (pill, indicating dose is each 2 balls, High Performance Liquid Chromatography/Mass Spectrometry method measurement result does not detect steroidal anti-inflammatory drugs.）
Get 2 balls, porphyrize mixes, be transferred to completely in the tool plug test tube that fills 4ml extraction organic solvent (10% potassium hydroxide methanol solution-absolute ethyl alcohol (10:90)), jolting 1min, with the disposable syringe of 5ml, draw about 3ml, with organic system miillpore filter, filter, filter 2 parts of each about 1ml, put in 2 colourless transparent glass bottles, portion adds about 0.2ml developer (0.2% TTC ethanol solution) as test solution wherein, the solution in contrast that does not add developer, mixes 2 parts of solution sealings observe phenomena after placement 3min.Result contrast solution is faint yellow, and it is faint yellow that test solution is, and dark unlike contrast liquid color, being negative property, shows that sample does not contain steroidal anti-inflammatory drugs, and this result confirms that with mass spectrum result is consistent.
embodiment 5
Cough dihydroxypropyltheophylline capsule (capsule, indicating dose is each 2, High Performance Liquid Chromatography/Mass Spectrometry method measurement result does not detect containing steroidal anti-inflammatory drugs.）
Get 2 capsules contents, join in the tool plug test tube that fills 4ml extraction organic solvent (10% potassium hydroxide methanol solution-absolute ethyl alcohol (10:90)), jolting 1min, with the disposable syringe of 5ml, draw about 3ml, with organic system miillpore filter, filter, filter 2 parts of each about 1ml, put in 2 colourless transparent glass bottles, portion adds about 0.2ml developer (0.2% TTC ethanol solution) as test solution wherein, the solution in contrast that does not add developer, 2 parts of solution sealings are mixed to observe phenomena after placement 3min.It is yellow that result contrast solution is, and it is yellow that test solution is, dark unlike contrast liquid color, is negative, and shows that sample does not contain steroidal anti-inflammatory drugs, and this result confirms that with mass spectrum result is consistent.
Claims (10)
1. a screening method for steroidal anti-inflammatory drugs in Chinese patent drug, health food, is characterized in that, comprises the steps: that successively (1) get 1 deal test sample and be placed in tool plug test tube, adds and extracts organic solvent 3 ~ 4ml, and jolting 1min, obtains test sample crude extract; (2) use organic system miillpore filter, filter out 2 parts of filtrates, each about 1ml, is placed in respectively 2 flint glass containers; (3) in a filtrate, add about 0.2ml tetrazole reagent as test solution wherein, another part is solution in contrast, and 2 parts of solution sealings are mixed; (4) 2 parts of solution are placed after 2 ~ 3min, in 3min, carried out colorimetric observation; (5) result judgement: if test solution is aobvious red and darker than contrast solution colour, be judged to be the positive, on the contrary negative;
1 described deal test sample is the test sample of a dose.
2. method according to claim 1, is characterized in that, described steroidal anti-inflammatory drugs has C
17-α-ol ketone group, has reductibility, can react with tetrazole test solution.
3. method according to claim 2, is characterized in that, described steroidal anti-inflammatory drugs is prednisone acetate, metacortandracin, hydrocortisone acetate, cortisone, hydrocortisone, dexamethasone acetate, the mixing of one or more in dexamethasone.
4. method according to claim 1, is characterized in that, the extraction organic solvent described in step (1) is alkaline ethanol solution, and this alkalescence one or more potpourris in NaOH, potassium hydroxide or tetrabutylammonium provide.
5. method according to claim 4, is characterized in that, first one or more potpourris in NaOH, potassium hydroxide or tetrabutylammonium is prepared to alkaline strong solution with absolute methanol, then mixes with ethanol; Described ethanol is the pure absolute ethyl alcohol of top grade or ethanol,aldehyde free.
6. method according to claim 5, is characterized in that, the pH of the alkaline ethanol solution making is more than 13, and water cut is below 5%.
7. method according to claim 1, is characterized in that, the described tetrazole reagent of step (3) is TTC, any in blue tetrazolium or both mixing; This tetrazole reagent is mixed with 0.2% ~ 0.5% chromophoric solution with absolute ethyl alcohol, and keeps in Dark Place in brown reagent bottle.
8. method according to claim 7, is characterized in that, tetrazole reagent is TTC.
9. application rights requires the steroidal anti-inflammatory drugs quick testing reagent kit of method described in 1, it is characterized in that, comprise box body, in box body, be provided with at least one and fill the tool plug test tube that 4ml extracts solvent, at least one fills the brown reagent bottle of 1ml tetrazole reagent, at least two water white reaction bulbs, the disposable syringe of at least one 5ml, and organic system miillpore filter.
10. whether detection method claimed in claim 1 mixes the application in the adrenocorticomimetic screening test of steroidal in Chinese patent drug and health food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310569766.7A CN103616376A (en) | 2013-11-13 | 2013-11-13 | Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310569766.7A CN103616376A (en) | 2013-11-13 | 2013-11-13 | Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103616376A true CN103616376A (en) | 2014-03-05 |
Family
ID=50167082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310569766.7A Pending CN103616376A (en) | 2013-11-13 | 2013-11-13 | Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103616376A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770230A (en) * | 2016-12-02 | 2017-05-31 | 山东省食品药品检验研究院 | A kind of prednisone acetate method for quick |
| CN106814065A (en) * | 2017-01-18 | 2017-06-09 | 山东省食品药品检验研究院 | A kind of hydrocortisone method for quick |
| CN108007926A (en) * | 2017-12-01 | 2018-05-08 | 温州科技职业学院 | The quick determination method of glucocorticoid and application in a kind of cosmetics |
| CN109490292A (en) * | 2018-11-23 | 2019-03-19 | 北京倍肯恒业科技发展股份有限公司 | The rapid detection method of biguanide compound in a kind of health care product |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692079A (en) * | 2009-08-28 | 2010-04-07 | 广东省汕尾市药品检验所 | Rapid detection method and rapid detection kit of fluoroquinolone antibiotics |
| CN103293153A (en) * | 2013-05-20 | 2013-09-11 | 韶关市食品药品检验所 | Rapid screening method for illegal addition of diclofenac sodium in anti-rheumatism Chinese patent medicines or healthcare products |
| CN103335969A (en) * | 2013-07-04 | 2013-10-02 | 王惠萱 | Colorimetric determination method for 17-hydoxycorticosteroid |
-
2013
- 2013-11-13 CN CN201310569766.7A patent/CN103616376A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692079A (en) * | 2009-08-28 | 2010-04-07 | 广东省汕尾市药品检验所 | Rapid detection method and rapid detection kit of fluoroquinolone antibiotics |
| CN103293153A (en) * | 2013-05-20 | 2013-09-11 | 韶关市食品药品检验所 | Rapid screening method for illegal addition of diclofenac sodium in anti-rheumatism Chinese patent medicines or healthcare products |
| CN103335969A (en) * | 2013-07-04 | 2013-10-02 | 王惠萱 | Colorimetric determination method for 17-hydoxycorticosteroid |
Non-Patent Citations (1)
| Title |
|---|
| 蔡美芳 主编: "《药物分析》", 30 June 1996, 中国医药科技出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770230A (en) * | 2016-12-02 | 2017-05-31 | 山东省食品药品检验研究院 | A kind of prednisone acetate method for quick |
| CN106814065A (en) * | 2017-01-18 | 2017-06-09 | 山东省食品药品检验研究院 | A kind of hydrocortisone method for quick |
| CN108007926A (en) * | 2017-12-01 | 2018-05-08 | 温州科技职业学院 | The quick determination method of glucocorticoid and application in a kind of cosmetics |
| CN109490292A (en) * | 2018-11-23 | 2019-03-19 | 北京倍肯恒业科技发展股份有限公司 | The rapid detection method of biguanide compound in a kind of health care product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103616376A (en) | Method for detecting steroidal anti-inflammation drugs in Chinese patent medicines and health-care products | |
| CN106706812A (en) | Method for detecting bufadienolide components in Liushen pill | |
| CN106645509A (en) | Detection method for antibiotic residue in milk | |
| CN111380855A (en) | Method for screening illegal drugs in health care products on site | |
| Dasgupta et al. | Effect of Brazilian, Indian, Siberian, Asian, and North American ginseng on serum digoxin measurement by immunoassays and binding of digoxin-like immunoreactive components of ginseng with Fab fragment of antidigoxin antibody (Digibind) | |
| Jeong et al. | Simultaneous UPLC-MS/MS determination of four components of Socheongryong-tang tablet in human plasma: Application to pharmacokinetic study | |
| CN105136966B (en) | A kind of quality determining method of Liang Fu Wan class preparation | |
| CN102393391A (en) | Rapid measurement method of melatonin doped in Chinese patent medicine and healthcare food | |
| CN101215313B (en) | Marsdenia tenacissima carbon-21 steroid saponin mixture with antineoplastic effect | |
| CN105510312A (en) | Orlistat rapid detection method | |
| CN105548161B (en) | A kind of quick determination method of Rimonabant | |
| CN101869630A (en) | Measurement method for content of atropine sulfate in traditional Chinese medicine suppository containing belladonna liquid extract | |
| CN103760292B (en) | A kind of method detecting undeclared prescription drugs in Small Meridian-Activating Pill | |
| CN101269097A (en) | Alstonia-leaf medicinal materials and medicament testing method for its preparation | |
| Dasgupta et al. | Rapid detection of oleander poisoning by Digoxin III, a new Digoxin assay: impact on serum Digoxin measurement | |
| Zheng et al. | Biosynthesis and pharmacokinetics of Panax notoginseng enteric-coated soft capsules | |
| CN102565046B (en) | Method for rapidly detecting mifepristone | |
| CN103743692A (en) | Rapid screening method for illegally added chemicals in small meridian-activating pills | |
| CN106525842A (en) | Quick detection method of dexamethasone | |
| CN106814065A (en) | A kind of hydrocortisone method for quick | |
| CN101185724B (en) | Quality control method of 'sifangweipian' | |
| CN102023157B (en) | Quick measurement method for pyrazolone substances doped in medicaments and health-care food | |
| CN106770230A (en) | A kind of prednisone acetate method for quick | |
| Hodgman et al. | The influence of polyethylene glycol solution on the dissolution rate of sustained release morphine | |
| Yu et al. | A rapid and low-cost approach to evaluate the allergenicity of herbal injection using HPLC analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140305 |
|
| WD01 | Invention patent application deemed withdrawn after publication |