CN103613494A - Technology for extracting coenzyme q10 by using Monascus mycelium as raw material and production method - Google Patents
Technology for extracting coenzyme q10 by using Monascus mycelium as raw material and production method Download PDFInfo
- Publication number
- CN103613494A CN103613494A CN201310577271.9A CN201310577271A CN103613494A CN 103613494 A CN103613494 A CN 103613494A CN 201310577271 A CN201310577271 A CN 201310577271A CN 103613494 A CN103613494 A CN 103613494A
- Authority
- CN
- China
- Prior art keywords
- monascus
- raw material
- ubiquinone
- anka nakazawa
- monascus anka
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 title claims abstract description 64
- 239000002994 raw material Substances 0.000 title claims abstract description 31
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 241000228347 Monascus <ascomycete fungus> Species 0.000 title abstract description 26
- 238000005516 engineering process Methods 0.000 title abstract description 3
- 238000000034 method Methods 0.000 claims abstract description 40
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000002525 ultrasonication Methods 0.000 claims abstract description 5
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 claims description 58
- 239000000284 extract Substances 0.000 claims description 33
- 244000113306 Monascus purpureus Species 0.000 claims description 32
- 235000002322 Monascus purpureus Nutrition 0.000 claims description 32
- 238000000605 extraction Methods 0.000 claims description 26
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 16
- 235000013305 food Nutrition 0.000 claims description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- 235000015895 biscuits Nutrition 0.000 claims description 8
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 8
- 208000001132 Osteoporosis Diseases 0.000 claims description 7
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 6
- 239000000654 additive Substances 0.000 claims description 6
- 230000000996 additive effect Effects 0.000 claims description 6
- 239000003208 petroleum Substances 0.000 claims description 6
- 208000031226 Hyperlipidaemia Diseases 0.000 claims description 5
- 235000013361 beverage Nutrition 0.000 claims description 4
- 235000012000 cholesterol Nutrition 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 238000005352 clarification Methods 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 229940057059 monascus purpureus Drugs 0.000 claims description 3
- 230000007935 neutral effect Effects 0.000 claims description 3
- 230000001681 protective effect Effects 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 3
- 235000011152 sodium sulphate Nutrition 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- -1 by sonioation method Substances 0.000 claims 1
- 238000000855 fermentation Methods 0.000 abstract description 29
- 230000004151 fermentation Effects 0.000 abstract description 29
- 240000007594 Oryza sativa Species 0.000 abstract description 13
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 13
- 235000009566 rice Nutrition 0.000 abstract description 12
- 238000011161 development Methods 0.000 abstract description 3
- 210000005260 human cell Anatomy 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 37
- 229940110767 coenzyme Q10 Drugs 0.000 description 34
- 239000000047 product Substances 0.000 description 21
- 238000003786 synthesis reaction Methods 0.000 description 16
- 239000003814 drug Substances 0.000 description 12
- 239000002893 slag Substances 0.000 description 9
- 239000002699 waste material Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000004040 coloring Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N methyl alcohol Substances OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 5
- PCZOHLXUXFIOCF-UHFFFAOYSA-N Monacolin X Natural products C12C(OC(=O)C(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 PCZOHLXUXFIOCF-UHFFFAOYSA-N 0.000 description 5
- 241000031003 Monascus ruber Species 0.000 description 5
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- PCZOHLXUXFIOCF-BXMDZJJMSA-N lovastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)[C@@H](C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 PCZOHLXUXFIOCF-BXMDZJJMSA-N 0.000 description 5
- 229960004844 lovastatin Drugs 0.000 description 5
- QLJODMDSTUBWDW-UHFFFAOYSA-N lovastatin hydroxy acid Natural products C1=CC(C)C(CCC(O)CC(O)CC(O)=O)C2C(OC(=O)C(C)CC)CC(C)C=C21 QLJODMDSTUBWDW-UHFFFAOYSA-N 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 241000208125 Nicotiana Species 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229960004756 ethanol Drugs 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229940035936 ubiquinone Drugs 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000000747 cardiac effect Effects 0.000 description 2
- 229960000935 dehydrated alcohol Drugs 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 102000005606 Activins Human genes 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- AFPLNGZPBSKHHQ-UHFFFAOYSA-N Betulaprenol 9 Natural products CC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCCC(C)=CCO AFPLNGZPBSKHHQ-UHFFFAOYSA-N 0.000 description 1
- 241000345998 Calamus manan Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000007189 Oryza longistaminata Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- 241000276707 Tilapia Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000003524 antilipemic agent Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000001851 biosynthetic effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 208000015606 cardiovascular system disease Diseases 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 235000012950 rattan cane Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- AFPLNGZPBSKHHQ-MEGGAXOGSA-N solanesol Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CC\C(C)=C\CO AFPLNGZPBSKHHQ-MEGGAXOGSA-N 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C46/00—Preparation of quinones
- C07C46/10—Separation; Purification; Stabilisation; Use of additives
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/80—Pastry not otherwise provided for elsewhere, e.g. cakes, biscuits or cookies
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/14—Organic oxygen compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention relates to a technology for extracting coenzyme q10 by using Monascus mycelium as a raw material and a production method. The Monascus mycelium is prepared from other different monascus strains by a solid or liquid fermentation method and does not contain Monascus haematochrome. After raw materials such as rice are subjected to liquid-submerged fermentation by using Monascus, a Monascus fermentation product is generated; after the Monascus haematochrome is extracted from the Monascus fermentation product by using 60%-70% ethanol, the rest of Monascus mycelium contains the important coenzyme q10 component which also exists in human cells and has an important health function; after the coenzyme q10 component is extracted by ultrasonication, the yield of the coenzyme q10 can reach 50-100mg/kg and has important industrial development value.
Description
It is technique and the production method that raw material extracts ubiquinone 10 that technical field the present invention relates to adopt Monascus anka Nakazawa et sato filament.
Background technology
Red colouring agent for food, also used as a Chinese medicine is at China production, the applicating history of existing more than 1,000 year, and it is the main bacteria seed of producing wine of rice fermented with red yeast, Red kojic rice, functional Monascus and natural pigment.In recent years, along with people are to synthetic foodstuff additive and the understanding of chemicals to human body toxic side effect, countries in the world have started edible green food and have used the upsurge of natural drug, and red colouring agent for food, also used as a Chinese medicine, as the natural product of dietotherapeutic, has caused great concern.From 1979, Japanese scholars rattan chapter far away is found after lovastatin physiologically active substance from monascus (monaseusruber), cause whole world scientist's concern, 1980～nineteen ninety peaks for red colouring agent for food, also used as a Chinese medicine functional study, and the function such as the reducing blood-fat of red colouring agent for food, also used as a Chinese medicine, decreasing cholesterol, hypotensive, hypoglycemic, germ resistance is found in succession.Merck company succeeds in developing first for clinical lovastatin lipid lowering agent, and in listing in 1987, oneself became the choice drug of hyperlipidemia, Coronary Heart Disease Patients current statins, and lipid lowerers has become second-biggest-in-the-world medicine.The current production technique of red colouring agent for food, also used as a Chinese medicine is mainly to design for extracting monascorubin, all generally that selection polished rice is raw material, adopt modern biotechnology to isolate the monascus ruber of high-quality, again through liquid submerged fermentation, produce red koji fermentation product, these products are through dehydration, the technique such as press dry, with 60～70% extraction using alcohol haematochrome, conventionally after these extraction process purification haematochrome, in red colouring agent for food, also used as a Chinese medicine, component for reducing blood fat lovastatin also withdraws together, the lovastatin that remaining slag stays seldom or does not almost have, in this production process, can produce a large amount of red colouring agent for food, also used as a Chinese medicine mycelia waste residues, these waste residues are generally considered as useless waste and discard, this has not only caused the pollution of environment, significant wastage to resource especially.We find in the detection of two batches of monascus waste residues that sky, Dongguan prebiotic thing scientific & technical corporation is produced, although finding, its waste residue can specificity not resist the lovastatin that suppresses HMC-CoA reductase activity, but lipid acid and polyose composition in addition in monascus waste residue, adopt these waste residues to carry out the experimentation on animals of reducing blood-fat and preventing osteoporosis, the extract of unexpected these waste residues of discovery also has the effect of good reducing blood lipid and preventing osteoporosis, our SEPARATE APPLICATION national inventing patent < < red rice residue product preparing the application > > of health-care food for lowering blood fat, (patent No. ZL201010003099.2, warrant book number the 1144784th), < < Monascus anka Nakazawa et sato filament extract is at the application > > (patent No. ZL201110269850.8 of preparation preventing osteoporosis protective foods and medicine, warrant book number the 1092336th), obtained the mandate of State Intellectual Property Office.Further research to the effective constituent of these Monascus anka Nakazawa et sato filaments, we find that these have extracted monascorubin mycelium afterwards and have also contained important component ubiquinone 10, this does not all have been reported at present all Research Literatures.
Coenzyme Q10 99.0 is a kind of fat-soluble antioxidant, it is one of indispensable important element of human life, the nutrition of energy human activin cell and cellular energy, have improve body immunity, strengthen anti-oxidant, delay senility and strengthen the functions such as human activity, medically be widely used in cardiovascular system diseases, extensively use it for dietary supplements and foodstuff additive both at home and abroad.Coenzyme Q10 99.0 is found in the U.S. early than nineteen fifty-seven, and within 1978, Peter doctor Mike of Univ Edinburgh UK is because of the contribution acquisition Nobel prize aspect research Coenzyme Q10 99.0 and cell energy relation.The phase early 1980s, Japan realized that from tobacco leaf, to extract solanesol be the synthetic Coenzyme Q10 99.0 of producing of raw material, to Coenzyme Q10 99.0 cost is significantly declined, and this application for Coenzyme Q10 99.0, universal and promote and played important pushing effect.The technical comparative maturity of semi-chemical synthesis, has realized industrialization, and product cost is low, moderate cost.Although but the product that uses semi-chemical synthesis to produce has superiority in price, in the use than having larger gap with the product that biological extraction method is produced.What reason was the production of biological extraction method is natural, organic product, the conversion that is easily absorbed by the body, is the synthetic organic products of artificial chemistry and chemical synthesis is produced, biological activity extreme difference, be difficult for being absorbed by the body, be difficult to give full play to the pharmacological action of Coenzyme Q10 99.0.It about Coenzyme Q10 99.0 chemical synthesis process, is the focus of studying both at home and abroad always, nearly half a century, developed country has realized Production by Microorganism Fermentation Coenzyme Q10 99.0, microorganism fermented extracted method had obtained significant progress in recent years, this brand-new biological engineering method, both combined the advantage of biological extraction technique and two kinds of methods of chemical synthesis process, overcome again their shortcoming, be therefore attract people's attention most be hopeful to realize industrialized method.
At present, the preparation method of Coenzyme Q10 99.0 mainly contains 3 kinds: extraction separation, chemical synthesis, biological synthesis process.Extraction separation extraction separation mainly extracts from animal livers, cardiac muscle etc. and oil bearing plant seed or tender shoots, tender leaf.According to the literature. through cell fragmentation, from Pigs Hearts slag and bovine cardiac slag, extract Coenzyme Q10 99.0 mensuration content and be respectively 75mg/kg and 73.3mg/kg, the content that extracts liver of hybrid tilapia Coenzyme Q10 99.0 by saponification method is 34mg/kg.With 10%KOH-methyl alcohol cell fragmentation, extracting the fluffy Coenzyme Q10 99.0 content of alkali is 63mg/kg.By soybean oil, extract Coenzyme Q10 99.0 and can reach 90mg/kg.Extraction method is production method comparatively traditional in modern biological project, and its production cost is higher, and raw material is also difficult to obtain, and from Reuse of Throwaway Material, extracting ubiquinone is current very popular research topic to reduce preparation cost.The chemical synthesis of Coenzyme Q10 99.0 can be divided into 2 kinds of full chemical synthesis and semi-chemical synthesis.Full chemical synthesis refers to that the Salanesol of not take from tobacco etc. is raw material, obtains the synthetic method of Coenzyme Q10 99.0 side chain completely by chemical process.The method raw material is cheap and easy to get, and product regioselectivity and stereoselectivity are high, but the structure of side chain need to repeatedly be coupled, and total recovery is low, is difficult to realize industrialization.Semi-chemical synthesis mainly contains side chain direct introduction method and side chain extends method, the method all need to be take natural Salanesol as critical materials synthesizing coenzyme Q 10, because the price of fine work and sterling Salanesol is very expensive, cause chemosynthesis Coenzyme Q10 99.0 with high costs, there is the shortcomings such as synthesis step is many, byproduct is many, chiral material separation difficulty simultaneously, therefore, chemical synthesis is subject to certain limitation in industrial production.Biological synthesis process is to utilize the vital movement of microorganism cells, vegetable cell or zooblast and the technical process that obtains people's desired substance.At present, the biosynthetic means of Coenzyme Q10 99.0 comprises culture plant cell and the two kinds of modes of microorganism cells fermentation utilized.Culture plant cell is mainly to take Tissues of Tobacco as material, by callus of induce, suspension culture, and generation fast growth, the tobacco suspension culturing cell that Coenzyme Q10 99.0 content is high.Have test-results to show, under applicable culture condition, NC89 tobacco cell output and Coenzyme Q10 99.0 content are respectively 16.653g/L and 14.780mg/L.The domestic unit that is engaged in Coenzyme Q10 99.0 culture plant cell only has several families, also all in the preliminary study stage, also do not enter pilot scale and suitability for industrialized production, 1977, Japan has realized microbe fermentation method suitability for industrialized production Coenzyme Q10 99.0 first, and the main bacteria seed that is applied to produce Coenzyme Q10 99.0 has photosynthetic bacterium, edaphic bacillus, red false born of the same parents bacterium, fission yeast etc.It is cheap abundant that the method has raw material. and product separation process is relatively simple, there is not the advantages such as chirality problem, the existing Some Enterprises of recent year adopts the method to produce, and applied for Patents, for example the Feng Yuan of Anhui Province medicine company group adopts Agrobacterium tumefaciens (Agrobacterium tumefaeiens) high density fermentation to produce Coenzyme Q10 99.0, but microbe fermentation method also needs development at the new bacterial strain of exploitation to improve the aspects such as fermentation efficiency, improvement later stage purification.From the feature of every kind of production method, biological synthesis process must be the main trend of future development, is also the study hotspot of current Coenzyme Q10 99.0 production method.
Our research is found, Monascus anka Nakazawa et sato filament is after we adopt sonioation method to extract, the content of Coenzyme Q10 99.0 can reach 50～100mg/kg, compare with raw material extracting method such as bovine cardiac slags with above-mentioned Pigs Hearts slag, having very favourable advantage, is a new invention that has very much commercial exploitation to be worth.
Summary of the invention
Find to adopt the raw materials such as rice to use monascus after liquid submerged fermentation, the red koji fermentation product producing, through with after 60～70% extraction using alcohol monascorubins, remaining monascus ruber filament has that important human body cell also exists, the Coenzyme Q10 99.0 composition with important nourishing function, this composition is by after adopting sonioation method to extract, and output can reach 50～100mg/kg, has important commercial exploitation and is worth.
The present invention mentions can be used for extracting the Monascus anka Nakazawa et sato filament raw material of Coenzyme Q10 99.0 can be by following method:
Method 1: get the raw material such as rice with monascus through liquid submerged fermentation after 7 to 10 days, the product liquid of the Fermentation Condition of Monascus spp of generation, removes water partial pressure by squeezing machine, then, 60～70% extraction using alcohol haematochrome, have extracted the slag of haematochrome, dry, pulverize as fine powder.
Method 2: get the raw material such as rice with monascus through liquid submerged fermentation after 7 to 10 days, the product liquid of the Fermentation Condition of Monascus spp of generation, removes water partial pressure by squeezing machine, then, 60～70% extraction using alcohol haematochrome, have extracted the slag of haematochrome, dry, by per kilogram, add 0.1～0.5 liter of 1% acetic acid, under 25 ℃ of room temperatures, maintain more than 24 hours, by 5～8 times of extracting in waters of gross weight, extract twice, each 1 hour, reclaim extracted twice liquid.The dry cream of simmer down to, pulverizes as fine powder room temperature preservation.
Method 3: get the raw material such as rice with monascus through liquid submerged fermentation after 7 to 10 days, the product liquid of the Fermentation Condition of Monascus spp producing, by squeezing machine, water partial pressure is gone, then, 60～70% extraction using alcohol haematochrome, extract the slag of haematochrome, dried, by per kilogram, added 0.1～0.5 liter of 1% sodium bicarbonate, under 25 ℃ of room temperatures, maintain more than 24 hours, by 5～8 times of extracting in waters of gross weight, extract twice, each 1 hour, reclaim extracted twice liquid, the dry cream of simmer down to, pulverizes as fine powder room temperature preservation.
Method 4: get the raw material such as rice with monascus through liquid submerged fermentation after 7 to 10 days, the product liquid of the Fermentation Condition of Monascus spp producing, by squeezing machine, water partial pressure is gone, then, 60～70% extraction using alcohol haematochrome, extracted the slag of haematochrome, dry, by 5～8 times of extracting in waters of gross weight, extract twice, each 1 hour, reclaim extracted twice liquid.The dry cream of simmer down to, pulverizes as fine powder room temperature preservation.
The Monascus anka Nakazawa et sato filament that the present invention mentions, also comprises with other Different Red Aspergillus strains through solid-state, or the Monascus anka Nakazawa et sato filament prepared of liquid state fermentation method.
Sonioation method extracts the technique of ubiquinone 10 from Monascus anka Nakazawa et sato filament: the Monascus anka Nakazawa et sato filament that takes aforesaid method acquisition is appropriate, adds acetone, ultrasonication in ice bath, the centrifugal 15min of 6000r/min (4 ℃).Get supernatant liquor, 35 ℃ of rotary evaporations are concentrated, then add 50ml petroleum ether dissolution; With ultrapure water 60ml washing, to neutral, get petroleum ether layer, add 5g anhydrous sodium sulphate, be dried to liquid clarification.Filter, filtrate Rotary Evaporators (37 ℃) is concentrated into dry, and volatilization completely, adds dehydrated alcohol 10ml to dissolve, and puts-20 ℃ of refrigerator freezings and separates out the impurity such as cholesterol, filters, and reclaims ethanol, obtains.With HPLC, detect the content of the ubiquinone 10 of this law extraction, testing conditions: detection wavelength is that 275nm, sample size are that 10 μ l, 30 ℃ of column temperatures, moving phase are methyl alcohol: ethanol (1:1), flow velocity are 1ml/min, with standard substance comparison, with the ubiquinone 10 that every gram of Monascus anka Nakazawa et sato filament of present method extracts, be 80～100 μ g, i.e. 80～100mg/kg.
Monascus anka Nakazawa et sato filament extracts ubiquinone 10 also can adopt supercritical CO
2extraction process extracts, when 30 ℃ of extraction temperature, and extracting pressure 22MPa, extraction 1.5h, CO
2during flow 4L/h, Monascus anka Nakazawa et sato filament extracts ubiquinone 10 extraction yields also can reach higher rate.
The Monascus anka Nakazawa et sato filament extract that the present invention extracts as stated above, can be used as in the production formula that biscuit additive adds biscuit, produce the biscuit or the protective foods that contain Monascus anka Nakazawa et sato filament ubiquinone 10, be provided with and suffer from hyperlipidemia, the patient of osteoporosis disease applies as heath food.At the addition of biscuit product, in 5～30% scopes, take 10～20% as good.
The ubiquinone 10 of the Monascus anka Nakazawa et sato filament that the present invention extracts as stated above, can be used as in the formula that drink additive adds beverage, produces the beverage that contains Monas cuspurpureus Went extract, is provided with and suffers from hyperlipidemia, and the patient of osteoporosis disease applies as heath food.At the addition of biscuit product, in 5～30% scopes, take 10～20% as good.
Embodiment:
Embodiment mono-:
Getting raw materials such as adopting rice uses monascus after liquid submerged fermentation, the red koji fermentation product producing, through with after 60～70% extraction using alcohol monascorubins, remaining monascus ruber filament is as the raw material that extracts ubiquinone 10, accurately take 5g mycelium, add acetone, ultrasonication in ice bath, the centrifugal 15min of 6000r/min (4 ℃).Get supernatant liquor, 35 ℃ of rotary evaporations are concentrated, then add 50ml petroleum ether dissolution; With ultrapure water 60ml washing, to neutral, get petroleum ether layer, add 5g anhydrous sodium sulphate, be dried to liquid clarification.Filter, filtrate Rotary Evaporators (37 ℃) is concentrated into dry, and volatilization completely, adds dehydrated alcohol 10ml to dissolve, and puts-20 ℃ of refrigerator freezings and separates out the impurity such as cholesterol, filters, and reclaims ethanol, obtains.This product detects Coenzyme Q10 99.0 content by HPLC, testing conditions: detection wavelength is that 275nm, sample size are that 10 μ l, 30 ℃ of column temperatures, moving phase are methyl alcohol: ethanol (1:1), flow velocity are 1ml/min.HPLC measures Coenzyme Q10 99.0 result through repeatedly repeating, and every gram of Monascus anka Nakazawa et sato filament can extract Coenzyme Q10 99.0 80～100 μ g, i.e. 80～100mg/kg.
Embodiment bis-:
Ultrasonication various amplitude extracts the impact of yield on Coenzyme Q10 99.0: get raw materials such as adopting rice and use monascus after liquid submerged fermentation, the red koji fermentation product producing, through with after 60～70% extraction using alcohol monascorubins, remaining monascus ruber filament is as the raw material that extracts ubiquinone 10, accurately take 5g mycelium, be made as 5 groups, inquire into respectively the treatment condition of ultrasonic cell-break, ultrasonic cell-break treatment condition are: each radiated time 10s, intermittent time is 15s, ultrasonic wave work total time is 10min, the amount of Monascus anka Nakazawa et sato filament is 5g, processing bacteria liquid amasss as under 30mL condition, amplitude is respectively 20 of peak power, 40, 60, 80, 100%, inquire into the various amplitude of best ultrasonic cell-break.Subsequent disposal, with embodiment mono-, the results are shown in Table 1:
Table 1: ultrasonic amplitude is to Monascus anka Nakazawa et sato filament extracting solution ubiquinone
10impact
From table 1 result, ultrasonic amplitude smudge cells is thought 100% the best.
Embodiment tri-:
Acetone add-on is on extracting the impact of Coenzyme Q10 99.0 being extracted to yield: get raw materials such as adopting rice and use monascus after liquid submerged fermentation, the red koji fermentation product producing, through with after 60～70% extraction using alcohol monascorubins, remaining monascus ruber filament is as the raw material that extracts ubiquinone 10, accurately take 5g mycelium, be made as 5 groups, selecting ultrasonic power output is 80%, each radiation/intermittent time of ultrasonic wave is 10/15s, ultrasonic wave work total time is 10min, respectively by 20, 25, 30, 35, 40mL, add acetone and carry out ultrasonic cell-break, observe and evaluate acetone add-on to extracting the impact of Coenzyme Q10 99.0 being extracted to yield, subsequent disposal is with embodiment mono-, the results are shown in Table 2:.
Table 2: acetone add-on is to Monascus anka Nakazawa et sato filament extracting solution ubiquinone
10impact
From table 2 result, acetone add-on is best with 20～25ml.
Claims (5)
1. Monascus anka Nakazawa et sato filament extracts the application of ubiquinone 10 as raw material.
2. technique and the production method of the extraction ubiquinone 10 that the Monascus anka Nakazawa et sato filament of take is raw material, is characterized in that the method comprises and take Monascus anka Nakazawa et sato filament as raw material, by sonioation method, extracts.
3. a kind of technique and production method of take the extraction ubiquinone 10 that Monascus anka Nakazawa et sato filament is raw material as claimed in claim 2, the extraction that it is characterized in that sonioation method is to take Monascus anka Nakazawa et sato filament as raw material, add acetone, ultrasonication, centrifugal, get supernatant liquor, 35 ℃ of rotary evaporations are concentrated, add Petroleum ether extraction; With ultrapure water washing, to neutral, get petroleum ether layer, add anhydrous sodium sulphate, be dried to liquid clarification, filter, filtrate Rotary Evaporators is concentrated into dry, adds anhydrous alcohol solution, puts-20 ℃ of refrigerator freezings and separates out the impurity such as cholesterol, filters, and reclaims ethanol, obtains.
4. the Monascus anka Nakazawa et sato filament as described in claim one extracts the application of ubiquinone 10 as raw material, it is characterized in that ubiquinone 10 that Monascus anka Nakazawa et sato filament extracts can be used as in the production formula that biscuit additive adds biscuit, produce the biscuit or the protective foods that contain Monascus anka Nakazawa et sato filament ubiquinone 10, be provided with and suffer from hyperlipidemia, the patient of osteoporosis disease applies as heath food.
5. the Monascus anka Nakazawa et sato filament as described in claim one extracts the application of ubiquinone 10 as raw material, it is characterized in that ubiquinone 10 that Monascus anka Nakazawa et sato filament extracts can be used as in the formula that drink additive adds beverage, produce the beverage that contains Monas cuspurpureus Went extract, be provided with and suffer from hyperlipidemia, the patient of osteoporosis disease applies as heath food.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310577271.9A CN103613494B (en) | 2013-11-05 | 2013-11-05 | Take Monascus anka Nakazawa et sato filament as technique and the production method that raw material extracts ubiquinone 10 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310577271.9A CN103613494B (en) | 2013-11-05 | 2013-11-05 | Take Monascus anka Nakazawa et sato filament as technique and the production method that raw material extracts ubiquinone 10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103613494A true CN103613494A (en) | 2014-03-05 |
| CN103613494B CN103613494B (en) | 2016-02-03 |
Family
ID=50164225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310577271.9A Active CN103613494B (en) | 2013-11-05 | 2013-11-05 | Take Monascus anka Nakazawa et sato filament as technique and the production method that raw material extracts ubiquinone 10 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103613494B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104591993A (en) * | 2014-12-30 | 2015-05-06 | 内蒙古金达威药业有限公司 | Method for extracting coenzyme Q10 from fermentation mycelia |
| CN104878053A (en) * | 2015-05-26 | 2015-09-02 | 南昌大学 | Preparation method of monascus metabolite |
| CN112457685A (en) * | 2020-12-01 | 2021-03-09 | 广东肇庆星湖生物科技股份有限公司 | Extraction and purification method of monascus orange pigment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101314782A (en) * | 2007-05-30 | 2008-12-03 | 宁波天安生物材料有限公司 | Method for fermentation preparation of cozymase Q10 |
| CN101381747A (en) * | 2008-10-28 | 2009-03-11 | 中国人民解放军军事医学科学院野战输血研究所 | Method for extracting coenzyme Q10 from microorganism |
| CN101491550A (en) * | 2008-01-22 | 2009-07-29 | 沈阳皓天万嘉医药科技有限公司 | Composite preparation of red rice extract and coenzyme Q10 and preparation method thereof |
| CN101836725A (en) * | 2010-01-01 | 2010-09-22 | 广东医学院 | Application of red rice residue product in preparing health-care food for lowering blood fat |
| CN102273637A (en) * | 2011-08-30 | 2011-12-14 | 广东医学院 | Application of monascus mycelium extracts to preparation of health-care food and medicines for preventing osteoporosis |
-
2013
- 2013-11-05 CN CN201310577271.9A patent/CN103613494B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101314782A (en) * | 2007-05-30 | 2008-12-03 | 宁波天安生物材料有限公司 | Method for fermentation preparation of cozymase Q10 |
| CN101491550A (en) * | 2008-01-22 | 2009-07-29 | 沈阳皓天万嘉医药科技有限公司 | Composite preparation of red rice extract and coenzyme Q10 and preparation method thereof |
| CN101381747A (en) * | 2008-10-28 | 2009-03-11 | 中国人民解放军军事医学科学院野战输血研究所 | Method for extracting coenzyme Q10 from microorganism |
| CN101836725A (en) * | 2010-01-01 | 2010-09-22 | 广东医学院 | Application of red rice residue product in preparing health-care food for lowering blood fat |
| CN102273637A (en) * | 2011-08-30 | 2011-12-14 | 广东医学院 | Application of monascus mycelium extracts to preparation of health-care food and medicines for preventing osteoporosis |
Non-Patent Citations (1)
| Title |
|---|
| 刘震等: "辅酶Q10联合血脂康治疗非酒精性脂肪性肝病的临床研究", 《中华中医药学刊》, vol. 29, no. 06, 30 June 2011 (2011-06-30), pages 1430 - 1431 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104591993A (en) * | 2014-12-30 | 2015-05-06 | 内蒙古金达威药业有限公司 | Method for extracting coenzyme Q10 from fermentation mycelia |
| CN104878053A (en) * | 2015-05-26 | 2015-09-02 | 南昌大学 | Preparation method of monascus metabolite |
| CN104878053B (en) * | 2015-05-26 | 2018-04-13 | 南昌大学 | A kind of preparation method of Metabolites ofMonascus |
| CN112457685A (en) * | 2020-12-01 | 2021-03-09 | 广东肇庆星湖生物科技股份有限公司 | Extraction and purification method of monascus orange pigment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103613494B (en) | 2016-02-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104672344B (en) | A kind of Entermorpha functional oligosaccharide zinc and preparation method thereof | |
| CN103263448B (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN102061220B (en) | Method for preparing onion functional components by using double-stage extraction based on subcritical water and solvent method | |
| CN105130939A (en) | Method for extracting luteolin from peanut shells | |
| CN102559828B (en) | Method for preparing astragaloside IV by converting total saponins of astragalus by microorganisms | |
| CN104529738B (en) | A kind of ubiquinone10extraction preparation method | |
| CN103613494B (en) | Take Monascus anka Nakazawa et sato filament as technique and the production method that raw material extracts ubiquinone 10 | |
| CN101440030B (en) | Supercritical preparation of conjugated linolic acid | |
| CN112057500A (en) | Method for extracting polysaccharide, volatile oil, flavone and lignan from schisandra chinensis | |
| CN102228503A (en) | Green and environment-friendly extraction method and applications of peanut shell polyphenol | |
| CN103276018A (en) | Method for producing lycopene by improving fermentation of Blakeslea trispora | |
| CN104522750A (en) | Alga healthcare food | |
| JP2015202065A (en) | Fermentation treatment product and method of producing product containing nobiletin and tangeretin in high contents | |
| CN104531822B (en) | A kind of purple sweet potato cyanidin efficiently synthesizes extracting method | |
| CN101012466A (en) | Method for preparing low-polyxylose by microwave treatment corn core enzymatical process | |
| CN103554287B (en) | A kind of extracting method of King Boletus mycelia polysaccharide | |
| CN108640956A (en) | A method of preparing flavonoid glycoside from tea seed | |
| CN101643403A (en) | Method for extracting linolenic acid from purslane | |
| CN1275545C (en) | Eatable ants and its preparation method | |
| CN102286565B (en) | Preparation method of theaflavin monomer | |
| CN105998222A (en) | Probiotic fermented unprocessed common monkshood daughter root composition and preparation method and application thereof | |
| CN1687239A (en) | Method for preparing crystal of lycopene and concentrate | |
| CN105803015A (en) | Method for converting dicentrine into (4S,6aR)-4-hydroxyl dicentrine through Clonostachys sp. Fermentation | |
| CN102727629B (en) | Method for extracting biological flavonoid by using citrus waste residues | |
| CN104561155A (en) | Flavobacterium-based fermentation method for producing vitamin K2 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 523808 Guangdong province Dongguan City Songshan Lake Science and Technology Park Metro output Road No. 1, Guangdong Medical College School of Medicine Patentee after: Guangdong Medical University Address before: 523808 Guangdong province Dongguan City Songshan Lake Science and Technology Park Metro output Road No. 1, Guangdong Medical College School of Medicine Patentee before: Guangdong Medical College |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20161228 Address after: 524023 Guangzhou, Huadu District, Xinhua Town, Fenghuang North Road, No., Feng Shang business building, No. 1306, No. 10 Patentee after: GUANGDONG RUNHE BIOTECHNOLOGY Co.,Ltd. Address before: 523808 Guangdong province Dongguan City Songshan Lake Science and Technology Park Metro output Road No. 1, Guangdong Medical College School of Medicine Patentee before: Guangdong Medical University |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: GUANGDONG RUNHE BIOTECHNOLOGY Co.,Ltd. Document name: Notification of Passing Examination on Formalities |
|
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 510800 Room 405 (Airport Huadu), No. 3, Lugang 7th Street, Huadu District, Guangzhou, Guangdong Province Patentee after: GUANGDONG RUNHE BIOTECHNOLOGY Co.,Ltd. Address before: No. 1306, Fengshang business building, No. 10, Fenghuang North Road, Xinhua Town, Huadu District, Guangzhou, Guangdong 524023 Patentee before: GUANGDONG RUNHE BIOTECHNOLOGY Co.,Ltd. |