CN103611191B - Moldable autogenous bone mud and preparation method thereof - Google Patents
Moldable autogenous bone mud and preparation method thereof Download PDFInfo
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- CN103611191B CN103611191B CN201310620160.1A CN201310620160A CN103611191B CN 103611191 B CN103611191 B CN 103611191B CN 201310620160 A CN201310620160 A CN 201310620160A CN 103611191 B CN103611191 B CN 103611191B
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Abstract
The invention relates to the technical field of cavity filling composite materials, and specifically relates to moldable autogenous bone mud and a preparation method thereof. The moldable autogenous bone mud comprises the following components: autogenous bone powder, powder of total saponin of panax notoginseng, human cryoprecipitate freeze-dried powder and calcium chloride liquor, wherein the volume ratio of the autogenous bone powder, the powder of total saponin of panax notoginseng to the human cryoprecipitate freeze-dried powder is (1-2):(1-2):(1-2). The preparation method of the moldable autogenous bone mud comprises the following steps: uniformly mixing the powder of total saponin of panax notoginseng and the human cryoprecipitate freeze-dried powder both radiated by a Co60-gamma ray; then, adding the calcium chloride liquor; uniformly mixing; and then uniformly mixing with the autogenous bone powder to obtain the moldable autogenous bone mud. The moldable autogenous bone mud provided by the invention has the advantages of easiness in molding, good molding effect, easiness for being fixed and good bacteriostatic action, and can promote healing of a postoperative area of mastoidectomy, so that the wound is quick to heal and smooth.
Description
Technical field
The present invention relates to art chamber filling technical field of composite materials, be specifically related to a kind of shaping Autogenous bone dust and preparation method thereof.
Background technology
On clinical medicine, otology benign lesion as chronic suppurative otitis media etc. for radical mastoidectomy combines tympanoplastic operative indication.But because art chamber is comparatively large in traditional operation, postoperative to there is auditory rehabilitation undesirable, and post-operative recovery time is long, and dry ear rate is low, the shortcoming that relapse rate is high.Therefore, most both at home and abroad otologists proposes: Mastoid obliteration should as one of important step of radical mastoidectomy.
Autologous bone powder is as Mastoid obliteration material the most frequently used clinically, autologous bone powder is thought the optimum selection of Mastoid obliteration material by numerous scholar, it have draw materials conveniently, osteogenesis and the advantage such as bone conductibility is good, immunoreactivity is low, but actual operation and postoperative to the process of patient follow-up in we find, the application still Shortcomings part of simple autologous bone powder, wherein the most outstanding 2 are: the first, autologous bone powder is difficult to moulding well in the field of operation that transudate is more, and art plot structure is rebuild effect and is therefore affected; The second, autologous bone powder is difficult to be fixed well, in art, postoperatively easily scatters and causes the infection of art district, granulation tissue hyperplasia, dry ear time lengthening, even increasing the risk of second operation.And, the autologous bone powder selected in operation underwent operative microscopic examination must be shown as healthy bone meal, if be mixed with focus in bone meal, the bone meal as cholesteatoma epithelium, granulation tissue etc. will by reject, therefore, collected bone meal capacity can not meet the demand in Reconstruction chamber.
Summary of the invention
An object of the present invention is for the deficiencies in the prior art, a kind of shaping Autogenous bone dust is provided, this shaping Autogenous bone dust has bacteriostasis, and this shaping Autogenous bone dust overcomes the simple shortcoming using autologous bone powder easily to scatter, be difficult to setting, off-capacity.
Two of object of the present invention is for the deficiencies in the prior art, a kind of preparation method of shaping Autogenous bone dust is provided, it is simple that the preparation method of this shaping Autogenous bone dust has method, and prepared shaping Autogenous bone dust can mould arbitrary shape, and the risk of second operation can be greatly reduced.
One of to achieve these goals, the present invention adopts following technical scheme:
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
Wherein, the volume ratio of described autologous bone powder, described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1 ~ 2:1 ~ 2:1 ~ 2;
Wherein, the volume ratio of the mixture of described calcium chloride solution and described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1:3 ~ 5.
Preferably, the volume ratio of described autologous bone powder, described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1:1:1.
The mass percent concentration of described calcium chloride solution is 4% ~ 6%.
Preferably, the mass percent concentration of described calcium chloride solution is 5%.
The purity of described Radix Notoginseng total arasaponins powder is 80% ~ 90%.
Preferably, the purity of described Radix Notoginseng total arasaponins powder is 85%.
To achieve these goals two, the present invention adopts following technical scheme:
The preparation method of a kind of shaping Autogenous bone dust described above, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by people's cryoprecipitate vacuum freeze-drying process at a certain temperature certain hour, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that certain radiation dose is obtained to Radix Notoginseng total arasaponins powder and step one, the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder will reach certain total number of bacteria;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
In technique scheme, in described step one, the temperature of vacuum freeze-drying process is set to-58 DEG C ~-55 DEG C, and the time of vacuum freeze-drying process is 7 hours ~ 9 hours.
In technique scheme, in described step 2, the gamma-ray radiation dose of described Co60-is 14kGy ~ 16kGy.
In technique scheme, in described step 2, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g.
Compared with prior art, beneficial effect is in the present invention:
1) a kind of shaping Autogenous bone dust provided by the invention, both the original advantage of autologous bone powder had been maintained, overcome again the simple shortcoming using autologous bone powder easily to scatter, be difficult to setting, there is easily moulding, moulding effective advantage, even if this shaping Autogenous bone dust also can be moulding well in the field of operation that transudate is more, art plot structure is rebuild effective.
2) a kind of shaping Autogenous bone dust provided by the invention, is easily fixed, and therefore, in art, is postoperatively not easy wandering thus can avoids the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening, and can greatly reduce the risk of second operation.
3) a kind of shaping Autogenous bone dust provided by the invention, in its component except autologous bone powder, also comprise most Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder, therefore, substantially increase the capacity of shaping Autogenous bone dust, make it possible to the demand meeting Reconstruction chamber.
4) a kind of shaping Autogenous bone dust provided by the invention, component comprises Radix Notoginseng total arasaponins powder, and containing more than 20 kind of Saponin active substance, 17 kinds of trace element, rich in protein, vitamin and polysaccharide etc. in Radix Notoginseng total arasaponins, modern pharmacology research shows, Radix Notoginseng total arasaponins significantly can reduce surface activity of blood platelet, anticoagulant and sticking, antithrombus formation, improve microcirculation, and the cerebral edema can significantly improved during global brain ischemia or focal cerebral ischemia-reperfusion injury, the propagation of people's bone marrow granulocyte, hemopoietic progenitor cell of red blood cell line can be promoted; And, carry out extracorporeal bacteria inhibitor test through applicant to Radix Notoginseng total arasaponins to show, Radix Notoginseng total arasaponins powder has bacteriostasis to the bacterial infection that chronic suppurative otitis media part is common, being responsive to proteus mirabilis, the antibacterial sensitivity of Staphylococcus albus, is intermediary's degree to the antibacterial sensitivity of staphylococcus aureus.Therefore, because shaping Autogenous bone dust of the present invention is using Radix Notoginseng total arasaponins powder as one of key component, thus make this shaping Autogenous bone dust not only have good bacteriostasis, and promote post-operative recovery.
5) a kind of shaping Autogenous bone dust provided by the invention, finds through zoopery, and this shaping Autogenous bone dust has the effect promoting filling district bone repair, and therefore, this shaping Autogenous bone dust can promote the healing in the postoperative art district of mastoid process in clinical practice.
6) a kind of shaping Autogenous bone dust provided by the invention, component comprises people's cryoprecipitate freeze-dried powder, and the blood plasma extract that it is main component that people's cryoprecipitate refers to platelet cofactor Ⅰ and Fibrinogen; Infusion people cryoprecipitate can supplement of coagulation factors VIII, vWF ELISA, Fibrinogen, fibrin stabilizing factor etc., and the fibronectin splicing variants contained in people's cryoprecipitate can cicatrize a wound soon and very smooth.Therefore, because shaping Autogenous bone dust of the present invention is using people's cryoprecipitate freeze-dried powder as one of key component, thus this shaping Autogenous bone dust can be cicatrized a wound soon and very smooth.
7) preparation method of a kind of shaping Autogenous bone dust provided by the invention, there is simple, the maneuverable advantage of method, and this preparation method makes it possible to according to operation actual requirement now with the current, and the stability of obtained shaping Autogenous bone dust can be ensured.
8) preparation method of a kind of shaping Autogenous bone dust provided by the invention, the shaping Autogenous bone dust obtained by this preparation method is utilized to show through scanning electron microscope, obtained shaping Autogenous bone dust has the advantage of good evenness, and the aperture of this shaping Autogenous bone dust is 1 μm ~ 4 μm, porosity is about 34.5%, just because of obtained shaping Autogenous bone dust, there is above-mentioned physical condition, thus be conducive to growing into of osteoblast and new capillary vessel.
Accompanying drawing explanation
Fig. 1 for clinically because of during chronic suppurative otitis media row radical mastoidectomy electric drill polishing mastoid region collected by autologous bone powder pictorial diagram.
Fig. 2 is the pictorial diagram of the shaping Autogenous bone dust obtained by embodiment 1 of a kind of shaping Autogenous bone dust of the present invention and preparation method thereof.
Fig. 3 is the scanning electron microscope (SEM) photograph (SEM, 15.0kV, × 30) of the shaping Autogenous bone dust obtained by embodiment 1 of a kind of shaping Autogenous bone dust of the present invention and preparation method thereof.
Fig. 4 is the scanning electron microscope (SEM) photograph (SEM, 15.0kV, × 40 000) of the shaping Autogenous bone dust obtained by embodiment 1 of a kind of shaping Autogenous bone dust of the present invention and preparation method thereof.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although done to explain to the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.
Powder mentioned by the present invention all refers to that size is less than the aggregation of the discrete particle of 1mm.
embodiment 1.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:1:1;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:4, and the mass percent concentration of the calcium chloride solution of the present embodiment is 5%, and the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 85%.The present embodiment Radix Notoginseng total arasaponins powder used is provided by Jing Zhu bio tech ltd, Nanjing.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 8 hours at-58 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 15kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 1 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 2.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1.5:2:1.5;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:3, and the mass percent concentration of the calcium chloride solution of the present embodiment is 4%, and the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 80%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 9 hours at-55 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 16kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 2 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 3.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 2:1.5:1.5;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:5, and the mass percent concentration of the calcium chloride solution of the present embodiment is 6%, and the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 90%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 7 hours at-57 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 14kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 3 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 4.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 2:1.5:2;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:3.5, and the mass percent concentration of the calcium chloride solution of the present embodiment is 4.5%, the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 87%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 8.5 hours at-56 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 15kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 4 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 5.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1.3:1.6:1.2;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:4.5, and the mass percent concentration of the calcium chloride solution of the present embodiment is 5.5%, the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 84%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 7.5 hours at-58 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 15kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 5 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 6.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1.7:1.3:1.8;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:4.8, and the mass percent concentration of the calcium chloride solution of the present embodiment is 5.2%, the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 83%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 8.5 hours at-57 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 16kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 6 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 7.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1.4:1.8:1.6;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:3.4, and the mass percent concentration of the calcium chloride solution of the present embodiment is 4.3%, the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 88%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 9 hours at-56 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 15kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 7 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
embodiment 8.
A kind of shaping Autogenous bone dust, it comprises following component: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
In the present embodiment, the volume ratio of autologous bone powder, Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1.6:1.4:1.3;
In the present embodiment, the volume ratio of the mixture of calcium chloride solution and Radix Notoginseng total arasaponins powder and people's cryoprecipitate freeze-dried powder is 1:3.8, and the mass percent concentration of the calcium chloride solution of the present embodiment is 5.7%, the purity of the Radix Notoginseng total arasaponins powder of the present embodiment is 86%.
The preparation method of above-mentioned a kind of shaping Autogenous bone dust, it comprises the following steps:
The preparation of step one, people's cryoprecipitate freeze-dried powder: by the vacuum freeze-drying process 8 hours at-58 DEG C of people's cryoprecipitate, obtain people's cryoprecipitate freeze-dried powder, and the people's cryoprecipitate freeze-dried powder room temperature lower seal obtained is saved backup; Wherein, people's cryoprecipitate that the present embodiment is used is provided by Guangzhou Blood Center;
Step 2, carry out radiation treatment with Co60-gamma-rays with people's cryoprecipitate freeze-dried powder that the radiation dose of 15kGy is obtained to Radix Notoginseng total arasaponins powder and step one, the total number of bacteria of the Radix Notoginseng total arasaponins powder after radiation treatment and people's cryoprecipitate freeze-dried powder is less than 10cfu/g;
The autologous bone powder of step 3, collection health of polishing with electric drill in operation, and it is for subsequent use to be placed in sterile chamber;
Step 4, according to formula ratio, by the Radix Notoginseng total arasaponins powder after the process of Co60-gamma-ray irradiation and the abundant mix homogeneously of people's cryoprecipitate freeze-dried powder, obtain mixture, then in mixture, add calcium chloride solution, and abundant mix homogeneously, obtain from preparing adhesive;
Step 5, according to formula ratio, fully will to mix homogeneously with autologous bone powder from preparing adhesive, namely to obtain shaping Autogenous bone dust.
The obtained shaping Autogenous bone dust of the present embodiment 8 has easily moulding, moulding effective, the advantage that is easily fixed, to make in art, be postoperatively not easy wandering thus the problem of the infection of art district, granulation tissue hyperplasia, dry ear time lengthening can be avoided, and the risk of second operation can be greatly reduced; And obtained shaping Autogenous bone dust has good bacteriostasis, the healing in the postoperative art district of mastoid process can be promoted, to cicatrize a wound soon and very smooth.The aperture of the shaping Autogenous bone dust obtained by the present embodiment is 1 μm ~ 4 μm, and porosity is about 34.5%, thus is conducive to growing into of osteoblast and new capillary vessel.
Claims (6)
1. a shaping Autogenous bone dust, is characterized in that: it is composed of the following components: autologous bone powder, Radix Notoginseng total arasaponins powder, people's cryoprecipitate freeze-dried powder, calcium chloride solution;
Wherein, the volume ratio of described autologous bone powder, described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1 ~ 2:1 ~ 2:1 ~ 2;
Wherein, the volume ratio of the mixture of described calcium chloride solution and described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1:3 ~ 5.
2. a kind of shaping Autogenous bone dust according to claim 1, is characterized in that: the volume ratio of described autologous bone powder, described Radix Notoginseng total arasaponins powder and described people's cryoprecipitate freeze-dried powder is 1:1:1.
3. a kind of shaping Autogenous bone dust according to claim 1, is characterized in that: the mass percent concentration of described calcium chloride solution is 4% ~ 6%.
4. a kind of shaping Autogenous bone dust according to claim 3, is characterized in that: the mass percent concentration of described calcium chloride solution is 5%.
5. a kind of shaping Autogenous bone dust according to claim 1, is characterized in that: the purity of described Radix Notoginseng total arasaponins powder is 80% ~ 90%.
6. a kind of shaping Autogenous bone dust according to claim 5, is characterized in that: the purity of described Radix Notoginseng total arasaponins powder is 85%.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903383A (en) * | 2004-07-26 | 2007-01-31 | 株式会社Gc | Bioabsorbable polymeric granular porous bone filling material and production method thereof |
| CN1927416A (en) * | 2006-09-29 | 2007-03-14 | 清华大学 | Composite porous calcium phosphate bone cement and method for making same |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903383A (en) * | 2004-07-26 | 2007-01-31 | 株式会社Gc | Bioabsorbable polymeric granular porous bone filling material and production method thereof |
| CN1927416A (en) * | 2006-09-29 | 2007-03-14 | 清华大学 | Composite porous calcium phosphate bone cement and method for making same |
Non-Patent Citations (1)
| Title |
|---|
| 乳突尖部分切除加自体骨粉鼓室上壁重建在鼓室成形术中的应用;陈学华 等;《新乡医学院学报》;20130430;第30卷(第4期);273-275，278 * |
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