CN103604760A - Method for determining organic matter content in soil - Google Patents
Method for determining organic matter content in soil Download PDFInfo
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- CN103604760A CN103604760A CN201310507227.0A CN201310507227A CN103604760A CN 103604760 A CN103604760 A CN 103604760A CN 201310507227 A CN201310507227 A CN 201310507227A CN 103604760 A CN103604760 A CN 103604760A
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Abstract
The invention provides a method for determination organic matter content in soil, and relates to a method for determining organic matters in soil, wherein the method is specifically applied for soil with organic matter content of less than or equal to 15%. A purpose of the present invention is to mainly solve problems of low accuracy and slow detection of the existing method for measuring organic matter content in soil. The determination method comprises: 1, placing a weighed air-dried soil sample in a digestion tube, and then adding to a potassium dichromate-concentrated sulfuric acid solution; 2, carrying out digestion on the soil solution; 3, preparing standard working solutions, wherein the standard working solutions comprise different contents of ferrous sulfate solutions and sulfuric acid solutions; 4, determining absorbance of the sample solution and the standard working solutions; and 5, drawing a standard curve, and substituting into a calculation formula to obtain the organic matter content in the soil. According to the method, the absorbance is adopted to replace the ferrous sulfate titration process so as to simplify the experiment steps, make the detection process be safe, and make the detection result be accurate.
Description
Technical field
The present invention relates to the assay method of soil with organic matter, be specifically applicable to the soil below soil organic matter content≤15%.
Background technology
At present, soil with organic matter content is one of important indicator of weighing soil fertility height, organic mass-energy impels soil texture to form, improve the condition of soil physics, chemistry and biological process, improve absorptive character and the shock-absorbing capacity of soil, organic matter itself contains again the needed various nutrients of plant simultaneously, as carbon, nitrogen phosphate and sulfur etc.Therefore, the fertility state of soil be understood, the mensuration of soil organic matter content must be carried out.
Soil with organic matter assay was to utilize the method for oil bath or sand-bath heating to accelerate organic oxidation in the past, make the oxidation of coal in the soil organism become carbon dioxide, and dichromic acid ion is reduced into trivalent chromic ion, ferrous standard solution titration for remaining potassium dichromate.But the method exists many deficiencies, first, oil belongs to inflammable thing, and the use of oil bath has increased the unsafe factor in laboratory; And sand-bath is in the heating inequality of being heated, make Organic oxidation degree different, in titration process, because change color also easily causes error, finally cause result inaccurate.In existing testing process that hence one can see that, affected by artificial factor larger, cause the accuracy that in prior art, the soil organism is measured lower.
Summary of the invention
The present invention will solve the low and slow problem of detection speed of the method accuracy of existing measurement soil with organic matter content, and a kind of method of the colorimetric method for determining soil organism is provided.
The method of the colorimetric method for determining soil organism of the present invention follows these steps to realize:
One, weigh: by decrement method, take the air-dry soil sample that 0.1000～0.2000g crosses 100 mesh sieve, then air-dry soil sample is placed in to disappear and boils pipe, with automatic zero burette, quantitatively adding the concentration of 5.00mL is 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, obtains the soil liquid;
Two, disappear and boil: on pipe, add a cover a curved neck funnel and be placed on and be preheated to 200～230 ℃ disappear and boil in stove disappearing to boil, from disappearing, boiling pipe puts into disappear to boil and stove, starts timing, after 8min, take out to disappear and boil pipe, be cooled to after room temperature, with syringe, by leak, to disappearing, boil in pipe adding distil water to 30mL, take off curved neck funnel, static placement 12～24h, the soil liquid that obtains disappearing after boiling;
Three, the preparation of standard operation solution: prepare 6 standard operation solution in the soil liquid described in preparation process one, getting 6 disappears and boils pipe respectively to add the concentration of 5.00mL be 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, then disappear for No. 1 and boil that in pipe, not add concentration be the copperas solution of C, to disappearing for No. 2, boil that in pipe, to add 2.5mL concentration be the copperas solution of C, to disappearing for No. 3, boil that in pipe, to add 5.0mL concentration be the copperas solution of C, to disappearing for No. 4, boil that in pipe, to add 7.5mL concentration be the copperas solution of C, to disappearing for No. 5, boil that in pipe, to add 10.0mL concentration be the copperas solution of C, to disappearing for No. 6, boil that in pipe, to add 12.5mL concentration be the copperas solution of C, to disappearing for No. 1, boil again that in pipe, to add 12.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 2, boil again that in pipe, to add 10.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 3, boil again that in pipe, to add 7.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 4, boil again that in pipe, to add 5.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 5, boil again that in pipe, to add 2.50mL concentration be the sulfuric acid solution of 0.1mol/L, disappear for No. 6 and boil pipe and do not add sulfuric acid solution,
Four, measure: using step 2 disappear boil the soil liquid after boiling that disappears in pipe supernatant as sample solution, together with the standard operation solution obtaining with step 3, with 1cm floating cuvette, 585nm place on spectrophotometer measures the absorbance that obtains sample solution absorbance and 6 groups of standard operation solution;
Five, the drafting of standard solution and result are calculated: the consumption milliliter number (V) that the X-axis of take is copperas solution, the absorbance drawing standard curve that the Y-axis of take is corresponding ferrous sulphate consumption milliliter number, by the consumption milliliter number (V of the corresponding copperas solution of linear equation calculation sample solution absorbance
1), in the following computing formula of substitution, obtain soil with organic matter content (O.M);
In above-mentioned formula:
O.M-soil organism massfraction, unit is (%);
The concentration of C-ferrous sulphate standard solution, unit is (mol/L);
V
1the milliliter Shuo， unit of the corresponding copperas solution of-sample solution absorbance is (mL);
The quality of m-air-dry soil sample, unit is (g).
The method of the colorimetric method for determining soil organism of the present invention is disappeared and is boiled pipe and substitute triangular flask and hard test tube by use, type of heating also boils stove and substitutes former methodical oil bath and sand-bath by disappearing, mensuration disappears and boils liquid and substitute ferrous sulphate titration process in 585nm place absorbance, and disappears and boil liquid absorbance stabilization time more than 3d.Both simplified experimental procedure, and reduced the consumption of reagent, and improved work efficiency simultaneously, thereby made testing process safer, result is more accurate.Simplify the step of determination experiment, increased work efficiency, reduced experimental cost.
Accompanying drawing explanation
Fig. 1 is that embodiment mono-step 5 be take the consumption milliliter number (V) that X-axis is copperas solution, and the absorbance that the Y-axis of take is corresponding ferrous sulphate consumption milliliter number is drawn the typical curve obtaining.
Embodiment
Embodiment one: the method for the present embodiment colorimetric method for determining soil organism follows these steps to implement:
One, weigh: by decrement method, take the air-dry soil sample that 0.1000～0.2000g crosses 100 mesh sieve, then air-dry soil sample is placed in to disappear and boils pipe, with automatic zero burette, quantitatively adding the concentration of 5.00mL is 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, obtains the soil liquid;
Two, disappear and boil: on pipe, add a cover a curved neck funnel and be placed on and be preheated to 200～230 ℃ disappear and boil in stove disappearing to boil, from disappearing, boiling pipe puts into disappear to boil and stove, starts timing, after 8min, take out to disappear and boil pipe, be cooled to after room temperature, with syringe, by leak, to disappearing, boil in pipe adding distil water to 30mL, take off curved neck funnel, static placement 12～24h, the soil liquid that obtains disappearing after boiling;
Three, the preparation of standard operation solution: prepare 6 standard operation solution in the soil liquid described in preparation process one, getting 6 disappears and boils pipe respectively to add the concentration of 5.00mL be 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, then disappear for No. 1 and boil that in pipe, not add concentration be the copperas solution of C, to disappearing for No. 2, boil that in pipe, to add 2.5mL concentration be the copperas solution of C, to disappearing for No. 3, boil that in pipe, to add 5.0mL concentration be the copperas solution of C, to disappearing for No. 4, boil that in pipe, to add 7.5mL concentration be the copperas solution of C, to disappearing for No. 5, boil that in pipe, to add 10.0mL concentration be the copperas solution of C, to disappearing for No. 6, boil that in pipe, to add 12.5mL concentration be the copperas solution of C, to disappearing for No. 1, boil again that in pipe, to add 12.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 2, boil again that in pipe, to add 10.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 3, boil again that in pipe, to add 7.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 4, boil again that in pipe, to add 5.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 5, boil again that in pipe, to add 2.50mL concentration be the sulfuric acid solution of 0.1mol/L, disappear for No. 6 and boil pipe and do not add sulfuric acid solution,
Four, measure: using step 2 disappear boil the soil liquid after boiling that disappears in pipe supernatant as sample solution, together with the standard operation solution obtaining with step 3, with 1cm floating cuvette, 585nm place on spectrophotometer measures the absorbance that obtains sample solution absorbance and 6 groups of standard operation solution;
Five, the drafting of standard solution and result are calculated: the consumption milliliter number (V) that the X-axis of take is copperas solution, the absorbance drawing standard curve that the Y-axis of take is corresponding ferrous sulphate consumption milliliter number, by the consumption milliliter number (V of the corresponding copperas solution of linear equation calculation sample solution absorbance
1), in the following computing formula of substitution, obtain soil with organic matter content (O.M);
In above-mentioned formula:
O.M-soil organism massfraction, unit is (%);
The concentration of C-ferrous sulphate standard solution, unit is mole every liter (mol/L);
V
1the milliliter Shuo， unit of the corresponding copperas solution of-sample solution absorbance is milliliter (mL);
The quality of m-air-dry soil sample, unit is (g).
Computing formula described in present embodiment step 5
in 0.003 to be carbon mM quality 0.012 removed by electron exchange number 4 in reaction, 1.724 in formula changes the average reduction coefficient of the soil organism, i.e. 100/58 ≈ 1.724 into for soil organic matter.
Present embodiment is disappeared and is boiled pipe and substitute triangular flask and hard test tube by use, type of heating also boils stove and substitutes former methodical oil bath and sand-bath by disappearing, mensuration disappears and boils liquid at the alternative ferrous sulphate titration process of 585nm place absorbance, has improved the accuracy that the soil organism is measured.
Embodiment two: present embodiment is different from embodiment one is that the quality of step 1 apoplexy dry soil samples is accurate to 0.0001g.Other step is identical with embodiment one.
Being accurate to air-dry soil sample that 0.0001g refers to mistake 100 mesh sieve of weighing and need being recorded to after radix point four described in present embodiment.
Embodiment three: present embodiment is different from embodiment one or two, and to be step 2 add a cover a curved neck funnel and be placed on and be preheated to 230 ℃ disappear and boil in stove disappearing to boil on pipe.Other step is identical with embodiment one or two.
Embodiment four: present embodiment is different from one of embodiment one to three is that step 3 boils to disappearing that to add concentration in pipe be the copperas solution of C, wherein concentration C=0.16mol/L～0.2mol/L.Other step is identical with one of embodiment one to three.
Embodiment mono-: the method that the standard model that the present embodiment employing soil organism massfraction is 1.21 ± 0.06% carries out the colorimetric method for determining soil organism follows these steps to realize:
One, weigh: by decrement method, take the air-dry soil sample that 0.1441g crosses 100 mesh sieve, then air-dry soil sample is placed in to disappear and boils pipe, with automatic zero burette, quantitatively adding the concentration of 5.00mL is 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, obtains the soil liquid;
Two, disappear and boil: on pipe, add a cover a curved neck funnel and be placed on and be preheated to 230 ℃ disappear and boil in stove disappearing to boil, from disappearing, boiling pipe puts into disappear to boil and stove, starts timing, after 8min, take out to disappear and boil pipe, be cooled to after room temperature, with syringe, by leak, to disappearing, boil in pipe adding distil water to 30mL, take off curved neck funnel, static placement 12h, the soil liquid that obtains disappearing after boiling;
Three, the preparation of standard operation solution: prepare 6 standard operation solution in the soil liquid described in preparation process one, getting 6 disappears and boils pipe respectively to add the concentration of 5.00mL be 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, then disappear for No. 1 and boil that in pipe, not add concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 2, boil that in pipe, to add 2.5mL concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 3, boil that in pipe, to add 5.0mL concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 4, boil that in pipe, to add 7.5mL concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 5, boil that in pipe, to add 10.0mL concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 6, boil that in pipe, to add 12.5mL concentration be the copperas solution of C=0.1695mol/L, to disappearing for No. 1, boil again that in pipe, to add 12.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 2, boil again that in pipe, to add 10.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 3, boil again that in pipe, to add 7.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 4, boil again that in pipe, to add 5.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 5, boil again that in pipe, to add 2.50mL concentration be the sulfuric acid solution of 0.1mol/L, disappear for No. 6 and boil pipe and do not add sulfuric acid solution,
Four, measure: using step 2 disappear boil the soil liquid after boiling that disappears in pipe supernatant as sample solution, together with the standard operation solution obtaining with step 3, with 1cm floating cuvette, 585nm place on spectrophotometer measures the absorbance that obtains sample solution absorbance and 6 groups of standard operation solution;
Five, the drafting of standard solution and result are calculated: the consumption milliliter number (V) that the X-axis of take is copperas solution, the absorbance drawing standard curve that the Y-axis of take is corresponding ferrous sulphate consumption milliliter number, by the consumption milliliter number (V of the corresponding copperas solution of linear equation calculation sample solution absorbance
1), in the following computing formula of substitution, obtain soil with organic matter content (O.M);
In above-mentioned formula:
O.M-soil organism massfraction, unit is (%);
The concentration of C-ferrous sulphate standard solution, unit is (mol/L);
V
1the milliliter Shuo， unit of the corresponding copperas solution of-sample solution absorbance is (mL);
The quality of m-air-dry soil sample, unit is (g).
Wherein the compound method of the 0.4mol/L potassium dichromate-concentrated sulfuric acid solution described in the present embodiment is to be transferred in 5L beaker with the 250mL beaker potassium chromate 80g that weighs, add deionized water to beaker 2L groove place, stirring makes it to dissolve, and then to add 2L density be 1.84g/cm
3the concentrated sulphuric acid, obtain 0.4mol/L potassium dichromate-concentrated sulfuric acid solution.
The copperas solution that the concentration that the present embodiment step 3 is used is C need to be demarcated, and scaling method is as follows:
Take 56g ferrous sulphate and be placed in 1L beaker, with wash bottle, rinse beaker more than three times, washing fluid is also incorporated in 1L beaker, finally adds deionized water to beaker 1L groove place.With 20mL graduated cylinder, measuring density is 1.84g/cm
3concentrated sulphuric acid 15mL, add in beaker.After stirring and dissolving, add deionized water to beaker 1L groove place.The normal concentration of this solution is demarcated with 0.2mol/L potassium dichromate standard solution.
Demarcate: with corrected 20mL pipette, draw 0.200mol/L potassium dichromate standard solution 20.00mL in 150mL triangular flask, with 10mL measuring cup, measuring the 3mL concentrated sulphuric acid adds in triangular flask, add 3 of Phen indicator, with the titration of ferrous sulphate standard solution, according to copperas solution consumption, pass through
obtain concentration, wherein C is the concentration (mol/L) of ferrous sulphate standard solution, C
1for the concentration (mol/L) of potassium dichromate standard solution, V
1for drawing the volume (mL) of potassium dichromate standard solution, V
2during for titration, consume the volume (mL) of ferrous sulphate standard solution.
The present embodiment step 5 be take the consumption milliliter number (V) that X-axis is copperas solution, the Y-axis of take consumes the absorbance drawing standard curve of milliliter number as corresponding ferrous sulphate, the consumption milliliter number of copperas solution is respectively 0mL, 2.5mL, 5mL, 7.5mL, 10mL and 12.5mL, corresponding absorbance is 0.000,0.033,0.063,0.098,0.122 and 0.148, thereby the typical curve obtaining is shown in Fig. 1, the equation y=0.0119x+0.0025 of Fig. 1 Plays curve, the coefficient R of curve
2=0.9968.
According to the method for the colorimetric method for determining soil organism of embodiment mono-, take respectively 4 parts of air-dry soil samples that quality is different again and carry out content of organic matter test, its quality is followed successively by 0.1002g, 0.1101g, 0.1321g, 0.1321g.And then obtain 5 parts of all product of wind desiceted soil.
By step 4, use 1cm floating cuvette 585nm place mensuration on spectrophotometer to obtain above 5 duplicate samples solution absorbances and be respectively 0.025,0.019,0.020,0.025,0.024.
Comparative example one: the method that the standard model that the present embodiment employing soil organism massfraction is 1.21 ± 0.06% carries out the conventional determining soil organism follows these steps to realize:
One, weigh: the air-dry soil sample of standard model that took 100 mesh sieve by decrement method is placed in respectively 150mL triangular flask, add pulverous silver sulfate 0.1g, with suction pipe, adding 5.00mL concentration is again 0.8000mol/L potassium dichromate standard solution, then with syringe, inject the concentrated sulphuric acid that 5mL mass concentration is 98%, rotation shakes up rear stand-by;
Two, disappear and boil: oil bath pan is heated to 190 ℃ in advance, the Boiling tube that step 1 is filled to the air-dry soil sample of standard model inserts in iron wire cage, then put it in oil cauldron and heat, control kettle temperature to 190 ℃, and make solution keep boiling 5min, then take out after test tube is cooling, with clean paper, clean the fluid of test tube outside;
Three, titration: be cooled to after room temperature in vitro potpourri and wash in 250mL conical flask, make bottle inner volume in 70mL left and right, add 3 of phenanthroline indicator, with the titration of ferrous sulphate standard solution, solution by orange through bluish-green to brownish red, be terminal; Record ferrous sulphate consumption (V); When analyzing, must do 2～3 blank demarcation, blank demarcation is not add air-dry soil sample, but adds 0.2g silica sand, and other steps are identical during with mensuration soil sample, record ferrous sulphate consumption (V
0);
Four, result is calculated: according to formula:
Calculate soil organism massfraction, in formula:
O.M---soil organism massfraction, unit is (%);
C---the concentration of ferrous sulphate standard solution, unit is mole every liter (mol/L);
V
0---blank test solution consumes the volume of ferrous sulphate standard solution, and unit is milliliter (mL);
V---sample test solution consumes the volume of ferrous sulphate standard solution, and unit is milliliter (mL);
The sample weighting amount of m---sample, unit is (g).
Wherein 0.003 for carbon mM quality 0.012 by electron exchange number 4 in reaction remove, 1.724 change the average reduction coefficient of the soil organism, i.e. 100/58 ≈ 1.724 into for soil organic matter.
This comparative example step 1 takes respectively 5 parts of air-dry soil samples as sample, and the quality of 5 parts of air-dry soil samples is respectively 0.2883g, 0.2004g, 0.2202g, 0.2642g and 0.2343g.
The concentration of the ferrous sulphate standard solution that this comparative example step 3 is used is 0.1695mol/L.
0.1695mol/L ferrous sulphate standard solution titration for this comparative example step 3, solution by orange through bluish-green to brownish red, be terminal, the data that record ferrous sulphate consumption (V) are respectively 18.10mL, 19.42mL, 19.00mL, 18.47mL, 18.81mL.
The ferrous sulphate consumption V that this comparative example step 3 record obtains
022.15mL.
To sum up two kinds of method testing results are as following table 1:
Table 1
Wherein sample number into spectrum A is for adopting the test result of 5 parts of standard model soil organism of embodiment mono-colorimetric method for determining, sample number into spectrum B is for adopting the test result of 5 parts of standard model soil organism of comparative example's one conventional method mensuration, the soil organism coefficient of variation that adopts colorimetric method for determining to obtain is 3.00%, demonstrates good accuracy.
Claims (4)
1. measure a method for soil organic matter content, it is characterized in that the method for the colorimetric method for determining soil organism follows these steps to realize:
One, weigh: by decrement method, take the air-dry soil sample that 0.1000～0.2000g crosses 100 mesh sieve, then air-dry soil sample is placed in to disappear and boils pipe, with automatic zero burette, quantitatively adding the concentration of 5.00mL is 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, obtains the soil liquid;
Two, disappear and boil: on pipe, add a cover a curved neck funnel and be placed on and be preheated to 200～230 ℃ disappear and boil in stove disappearing to boil, from disappearing, boiling pipe puts into disappear to boil and stove, starts timing, after 8min, take out to disappear and boil pipe, be cooled to after room temperature, with syringe, by leak, to disappearing, boil in pipe adding distil water to 30mL, take off curved neck funnel, static placement 12～24h, the soil liquid that obtains disappearing after boiling;
Three, the preparation of standard operation solution: prepare 6 standard operation solution in the soil liquid described in preparation process one, getting 6 disappears and boils pipe respectively to add the concentration of 5.00mL be 0.4mol/L potassium dichromate-concentrated sulfuric acid solution, then disappear for No. 1 and boil that in pipe, not add concentration be the copperas solution of C, to disappearing for No. 2, boil that in pipe, to add 2.5mL concentration be the copperas solution of C, to disappearing for No. 3, boil that in pipe, to add 5.0mL concentration be the copperas solution of C, to disappearing for No. 4, boil that in pipe, to add 7.5mL concentration be the copperas solution of C, to disappearing for No. 5, boil that in pipe, to add 10.0mL concentration be the copperas solution of C, to disappearing for No. 6, boil that in pipe, to add 12.5mL concentration be the copperas solution of C, to disappearing for No. 1, boil again that in pipe, to add 12.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 2, boil again that in pipe, to add 10.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 3, boil again that in pipe, to add 7.50mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 4, boil again that in pipe, to add 5.00mL concentration be the sulfuric acid solution of 0.1mol/L, to disappearing for No. 5, boil again that in pipe, to add 2.50mL concentration be the sulfuric acid solution of 0.1mol/L, disappear for No. 6 and boil pipe and do not add sulfuric acid solution,
Four, measure: using step 2 disappear boil the soil liquid after boiling that disappears in pipe supernatant as sample solution, together with the standard operation solution obtaining with step 3, with 1cm floating cuvette, 585nm place on spectrophotometer measures the absorbance that obtains sample solution absorbance and 6 groups of standard operation solution;
Five, the drafting of standard solution and result are calculated: the consumption milliliter that the X-axis of take is copperas solution is counted V, the absorbance drawing standard curve that the Y-axis of take is corresponding ferrous sulphate consumption milliliter number, counts V by the consumption milliliter of the corresponding copperas solution of linear equation calculation sample solution absorbance
1, in the following computing formula of substitution, obtain soil with organic matter content O.M;
In above-mentioned formula:
O.M-soil organism massfraction, unit is (%);
The concentration of C-ferrous sulphate standard solution, unit is (mol/L);
V
1the milliliter Shuo， unit of the corresponding copperas solution of-sample solution absorbance is (mL);
The quality of m-air-dry soil sample, unit is (g).
2. a kind of method of measuring soil organic matter content according to claim 1, is characterized in that the quality of step 1 apoplexy dry soil samples is accurate to 0.0001g.
3. a kind of method of measuring soil organic matter content according to claim 1, is characterized in that step 2 adds a cover a curved neck funnel and be placed on and be preheated to 230 ℃ disappear and boil in stove disappearing to boil on pipe.
4. a kind of method of measuring soil organic matter content according to claim 1, is characterized in that step 3 boils to disappearing that in pipe, to add concentration be the copperas solution of C, wherein concentration C=0.16mol/L～0.2mol/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106289940A (en) * | 2016-10-09 | 2017-01-04 | 农业部亚热带果品蔬菜质量监督检验测试中心 | A kind of method of quick mensuration soil organic matter content |
| CN106383199A (en) * | 2016-08-30 | 2017-02-08 | 中国烟草总公司广东省公司 | Method for determination of organic matters in tobacco floating-seedling production matrix |
| CN108828241A (en) * | 2018-03-15 | 2018-11-16 | 中国科学院合肥物质科学研究院 | A kind of soil nutrient automatic analysis system and its working method based on robot |
| CN108956492A (en) * | 2018-06-22 | 2018-12-07 | 广西大学 | A method of the measurement sludge content of organic matter |
| CN109916899A (en) * | 2019-04-24 | 2019-06-21 | 福建海峡环保集团股份有限公司 | Organic carbon and full nitrogen determination method in soil |
| CN109991389A (en) * | 2019-03-29 | 2019-07-09 | 中国科学院东北地理与农业生态研究所 | Organic carbon content measuring method in a kind of soil |
| CN112285030A (en) * | 2020-09-13 | 2021-01-29 | 中建三局第一建设工程有限责任公司 | Method for rapidly identifying organic matter content of river sludge |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2020481C1 (en) * | 1991-01-11 | 1994-09-30 | Санкт-Петербургский государственный университет | Method for determination of humus types |
| KR100295182B1 (en) * | 1998-11-19 | 2001-11-14 | 현 해 남 | Method for measuring the amount of organic material using spectrophotometer |
-
2013
- 2013-10-24 CN CN201310507227.0A patent/CN103604760A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2020481C1 (en) * | 1991-01-11 | 1994-09-30 | Санкт-Петербургский государственный университет | Method for determination of humus types |
| KR100295182B1 (en) * | 1998-11-19 | 2001-11-14 | 현 해 남 | Method for measuring the amount of organic material using spectrophotometer |
Non-Patent Citations (4)
| Title |
|---|
| 中国人民共和国农业部: "土壤有机质测定法", 《中华人民共和国国家标准 GB 9834-88》, 1 March 1989 (1989-03-01) * |
| 李优琴 等: "土壤有机质测定方法中消解条件的优化", 《江苏农业科学》, vol. 41, no. 9, 25 September 2013 (2013-09-25) * |
| 鲁如坤: "《土壤农业分析方法》", 31 December 2000, 中国农业科技出版社, article "土壤农业分析方法" * |
| 黄春兰 等: "土壤有机质的分光光度测定", 《热带农业科学》, no. 4, 31 December 1990 (1990-12-31) * |
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