CN103602596A - Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof - Google Patents
Monochamus alternates hope beauveria bassiana space mutant B305 and application thereof Download PDFInfo
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Abstract
The invention discloses a monochamus alternates hope beauveria bassiana space mutant B305 and an application thereof. The mutant B305 with significant improvements in the aspects of growth speed, spore yield, pathogenicity to long-horned beetles and other performances is screened by enabling a beauveria bassiana wild strain to be carried on a Shenzhou-8 spacecraft to perform space induction and taking a non-carried original strain as a control, and the microorganism accession number is CGMCC No. 8049. Pathogenicity and indoor control experiments show that the pathogenicity and the control effect against monochamus alternatus hopes of the space mutant B305 are significantly higher than those of the original beauveria bassiana strain. The invention also discloses a biocontrol preparation prepared from the monochamus alternates hope bassiana space mutant B305. The invention further provides an application of the monochamus alternates hope beauveria bassiana space mutant B305 and a microbial preparation of the mutant B305 in the aspect of controlling long-horned beetles and other pests.
Description
Technical field
The present invention relates to insect pathogenic fungus mutant, relate in particular to Monochamus alternatus Hope beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) space flight mutant strain, the invention further relates to the application of Monochamus alternatus Hope beauveria bassiana space flight mutant strain in control longicorn, belong to beauveria bassiana mutant strain and Application Areas thereof.
Background technology
By pine wood nematode Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle) pine nematode that causes is a kind of crushing pine tree disease, forest development in serious threat.In East Asia, this disease is mainly carried out natural propagation by Monochamus alternatus Hope (the Monochamus alternates Hope) adult after sprouting wings, and when supplementing the nutrients, passes on healthy pine tree, causes pine death.Monochamus alternatus Hope in China to the east of Taiwan, reach Guangdong in the south, to Tibet, ,Bei Zhi Hebei, northwest to the Qinling Mountains, northeast to Liaoning, all have distribution.Therefore, cut off the route of transmission of pine nematode, effectively reduce the population density of the brown black longicorn of its communication media insect pine, seem particularly important.
Beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) is a kind of insect pathogenic fungus [Li Zengzhi that China is most widely used, Fan Meizhen], Chinese scholars application muscardine control Monochamus alternatus Hope has been done a lot of research (Pu dragon that stings, Li Zengzhi. entomomycete is learned [M]. Hefei: Anhui Province science and technology press, 1996:271～361; Zhang Linghua, Tian Xingshan. the progress of microorganism space mutagenesis breeding [J]. nuclear agricultural science report, 2004,18 (4): 294-296; Song Mangang, is filled expensive, Miao Meisuo, and red dawn is auspicious, Liang Hong, Luo Baojun. and China's space breeding achievement is [J] significantly. plant subworld, 2004,7:57).Beauveria bassiana is the same with other insect pathogenic fungus, as biological pesticide, although there is the incomparable many merits of chemical pesticide, exist the time of lethal insect longer, also there is large, the shortcoming such as prevention effect is unstable and self resistance is poor affected by environment simultaneously.In order to change this circumstances, by high-level efficiency breeding to the physiological variation of microorganism and heritable variation, insect pathogenic fungus is transformed, and (agriculture is to group, Zhang Zehua for seed selection biological control strain excellent provides new way for Flight Mutagenesis, Hu Pan, Gao Song, Zhang Lisheng. Flight Mutagenesis is to the biological effect of insect pathogenic fungus [J]. fungus journal, 2006,25 (4): 674-681), and successfully screen good Metarhizium anisopliae space flight mutant.To obtain the product that is applicable to market demand.
Space environment has good mutagenesis, can produce improve bacterial classification favourable proterties, create the useful variations such as new variety, also can send out and bring out the quantitative variability relevant with output, but a lot of variation has certain randomness.The main purpose of carrying microorganism be exactly by design preferred plan screen those output high, be worth large variation and produce bacterial strain (Sun Jimei, fourth coral, Xiao Hua, Deng. the research [J] of beauveria bassiana control Monochamus alternatus. the communication of Forest Diseases worm, 1997 (3): 16-18), and in order to select to obtain new variety or the strain that proterties is stable, need to carry out a large amount of heavy screenings and detect test (Liu Hongjian, Piao Chungen, Wang Laifa, Deng. muscardine and swollen leg honeybee are to the effect of alternatus Larva [J]. forest-science, 2007,43 (5): 64 – 68; Zhang Liqin ，Liu army. Monochamus alternatus muscardine bacterial strain screening [J]. Nanjing Forestry University's journal, 2000,24 (2): 33-37).
Beauveria bassiana from the separation of Monochamus alternatus Hope larva health is carried out spaceship-carried, by Flight Mutagenesis, screen and obtain in production performance and the mutant strain being significantly increased on to longicorn virulence, for the biological control of longicorn, will have great importance.
Summary of the invention
Main purpose of the present invention is to carry out Flight Mutagenesis acquisition at production performance or mutant strain that longicorn virulence is significantly improved or is improved from the beauveria bassiana of Monochamus alternatus Hope larva health separation (Beauveria bassiana (Bals.) Vuill.);
Another object of the present invention is obtained mutant strain to be applied to the biological control of longicorn.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
Beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) the mutant strain B305 that one strain is significantly improved or improves in production performance or to longicorn virulence, its microbial preservation number is: CGMCC No.8049; Classification And Nomenclature: beauveria bassiana Beauveria bassiana; The preservation time: on August 20th, 2013; Depositary institution is: China Committee for Culture Collection of Microorganisms's common micro-organisms center; Preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
The present invention is divided into respectively two parts by beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) bacterial strain cfcc81357, and a stand-down routine preservation is with comparing (CK); No. 8 airships of another part of lift-launch Shenzhou last 6d20h and carry out Flight Mutagenesis, spaceship-carried bacterial strain returns behind ground, the original strain not carrying of take is contrast, more spaceship-carried bacterial strain is in the variability of the aspects such as table shape, pigment change, the speed of growth, sporulation quantity and virulence, to screen, obtains at growth performance or mutant strain that longicorn pathogenecity is significantly increased.
The present invention by microscopic examination Flight Mutagenesis for the impact in the formalness of beauveria bassiana; it is raw on vegetative hyphae that microscopic examination can see that the conidiophore of original strain; conidiogenous cell clusters on mycelia, conidiophore or the sporangiocyst that expands; doleiform; neck obviously extends into product spore axle; on axle, there is little tooth prominent; be "the" shape bending; conidiogenous cell and sporangiocyst Chang Zengsheng; on conidiophore or mycelia, conglomerate is to avette quite closely knit spore head, and conidium is spherical or subsphaeroidal.Mutant strain B130 mycelia generally vertically grows to same direction, and mycelia is long and straight, does not produce spore.The mycelia form of B159 is compared with original strain, and barrier film is obvious, and mycelia is slightly crooked between each barrier film.
The present invention has observed the impact of Flight Mutagenesis for strain growth speed, the speed of growth of discovery beauveria bassiana Flight Mutagenesis bacterial strain is compared with original strain, there is positive and negative two direction variation, but most bacterial strain all presents negative sense variation, only have less bacterial strain to present forward variation, variation amplitude is larger, illustrates that Flight Mutagenesis is all influential to the trend of the speed of growth of bacterium colony and amplitude.Each colony diameter has growth more than 2mm average every day, belongs to growth fraction type more rapidly; Cultivate after 10d, the colony diameter of bacterial strain B305 is all greater than original strain.Beauveria bassiana mutants which had culturing process mycelia dry weight rangeability is little, cultivates after 4d, and the B305 of the bacterial strain that mycelia dry weight is the heaviest is 0.8492g.
On the impact of bacterial strain sporulation quantity, there is the variation of different trends, different amplitudes in Flight Mutagenesis.That wherein sporulation quantity is maximum is bacterial strain B305, and its sporulation quantity is 2.12 * 10
7cfu/mL, and bacterial strain B130 does not produce spore, by mycelia morphologic observation, can illustrate that it is without Sporulation yet.
The present invention further investigates the impact of Flight Mutagenesis on virulence, and after inoculation, each mutant is different to the virulence of Monochamus alternatus Hope larva, and Flight Mutagenesis is larger on virulence impact, occurs the more of negative sense sudden change.Within the 4th day, larva starts death, and the virulence of mutant B305 is compared original strain and is significantly increased, and in lethal speed, also faster than original strain, illustrates that mutant B305 has stronger virulence to Monochamus alternatus Hope larva.
Indoor control effect is tested and is shown, B305 bacterial strain is significantly better than original Strain of Beauveria bassiana to the prevention effect of Monochamus alternatus Hope.
In general, beauveria bassiana, after Flight Mutagenesis, has produced abundant character variation.The negative effect of Flight Mutagenesis is greater than positive-effect, and a lot of proterties such as the speed of growth, sporulation quantity, virulence are all shown to restraining effect in various degree, and minority presents promoter action, has shown different variation trend and amplitude.The speed of growth, sporulation quantity and virulence are the important indicators of screening biocontrol strains, Flight Mutagenesis makes these property lists reveal Different Variation trend and amplitude, proved that Flight Mutagenesis is non-fixed point, popularity, positive and negative amphitropic, and mutagenesis site is many, induced mutation rate is high, the organism variation causing has physiological variation also may have inheritable variation.The present invention is the variability at aspects such as table shape, pigment change, the speed of growth, sporulation quantity and virulencies by the spaceship-carried bacterial strain of screening, find that forward variation is by a relatively large margin occurring aspect growth characteristics and virulence Flight Mutagenesis bacterial strain B305, be the bacterial strain that a strain has excellent mutant character, in the productions such as control of longicorn and research, be with a wide range of applications.
Therefore, the present invention also provides a kind of beauveria bassiana microbial preparation of preventing and treating longicorn, and described microbial preparation comprises: the spore powder of beauveria bassiana B305 or fermented liquid and preparations carrier.
As a reference, those skilled in the art can prepare beauveria bassiana B305 spore powder with reference to following method: (1) prepares beauveria bassiana B305 fermented liquid; (2) from beauveria bassiana B305 fermented liquid, reclaim spore product; (3) the dry beauveria bassiana spore product reclaiming, obtains.
Those skilled in the art can prepare beauveria bassiana B305 fermented liquid according to disclosed the whole bag of tricks in document; For example, adopt three grades of cultural methods, that is: seed culture, secondary fluid enlargement culture and liquid fermentation and culture, prepare beauveria bassiana B305 fermented liquid; Wherein, when liquid fermentation and culture, can adopt ferment tank or adopt the mode of shake-flask culture to carry out fermentation culture; Cultivate substratum used and culture condition is all open in the literature for three grades, as a reference, wherein, the moiety of described liquid fermentation medium comprises Carbon and nitrogen sources; Described carbon source includes but not limited to any one or more in glucose, sucrose, maltose, Zulkovsky starch or Semen Maydis powder.Described nitrogenous source includes but not limited to any one or more in SODIUMNITRATE, ammonium sulfate, peptone or analysis for soybean powder.
The present invention further provides a kind of method of preparing the beauveria bassiana B305 microbial preparation of preventing and treating longicorn, the method comprises the following steps: the spore powder of beauveria bassiana B305 or fermented liquid and carrier are mixed, stir, pulverize and sieve, prepare corresponding microbial preparation, for example, can be granular preparation, pulvis, wettable powder or microcapsule microbial agent etc.
Wherein, described carrier can be diatomite, kaolin, wood chip, activated carbon, the peat composed of rotten mosses, agricultural crop straw, dry farm manure etc.; In addition, in Paecilomyces lilacinus microbial preparation of the present invention, also auxiliary material can be added or/and auxiliary agent; Described auxiliary material can be crab shell powder or chitin etc.; Described auxiliary agent can be wetting agent, dispersion agent or stablizer etc.
Accompanying drawing explanation
The beauveria bassiana morphologic variation of Fig. 1 Flight Mutagenesis and original strain colonial morphology; A-I:B122, B130, B159, B260, B283, B305, BJ-1, BJ-80, wild-type.
The mycelial structure of Fig. 2 Flight Mutagenesis beauveria bassiana mutant.
Fig. 3 beauveria bassiana space flight mutant strain speed of growth and original strain comparison.
Fig. 4 beauveria bassiana space flight mutant strain mycelia dry weight and original strain comparison.
Fig. 5 beauveria bassiana space flight mutant strain sporulation quantity and original strain comparison.
Embodiment
By the following example, embodiments of the present invention will be more specifically described, it should be understood that described embodiment is only exemplary, does not form any restriction to scope of the present invention.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications or replacement all fall into protection scope of the present invention.
The screening of experimental example 1 beauveria bassiana Flight Mutagenesis mutant strain and the determination experiment of mutagenic effect
1 experiment material and method
1.1 bacterial strain
The alternatus Larva body surface separation and purification of infecting muscardine in beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) (bacterial strain cfcc81357) Lishui city, Xi Cong Jiangsu Province horse hair pine tree obtains.
For trying 4 ℃ of preservations of beauveria bassiana (Beauveria bassiana) Flight Mutagenesis ground return Hou，Yu Ben testing laboratory, standby.
1.2 spaceship-carried
Beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) bacterial strain cfcc81357 is divided into respectively to two parts, and a stand-down routine preservation is with comparing (CK); Another part carries No. 8 airships of Shenzhou on November 1st, 2011, lasts 6d20h, returns behind ground, takes out in time material at low temperature and preserves.
1.3 screening mutant
Strain of Beauveria bassiana after Flight Mutagenesis is processed and wild type strain (CK) are made respectively to spore suspension (B130 is mycelium), dilution spore concentration to 1 * 10
3cfu/mL, is applied to (every ware adds 0.1mL spore liquid) on PDA flat board, after 25 ℃ of constant temperature culture 4-6d, observes single bacterium colony size, form and pros and cons color, picks out and the obvious bacterial strain of wild type strain (CK) difference.
The mensuration of 1.4 Flight Mutagenesis effects
The original strain not carrying of take is contrast, and relatively space flight bacterial strain is in the variability of the aspects such as table shape, pigment change, the speed of growth, sporulation quantity and virulence.
1.4.1 the form of mutant strain and pigment change are observed
From the flat board of having grown, with the punch tool that diameter is 6mm, cut 3 of bacterium colonies and contain in the solution of 0.5 ‰ tweens in 5mL, fully concussion, makes spore suspension.With the filter paper that the diameter of the bacterium of having gone out is 6mm, dip in full spore liquid and be placed on culture dish central authorities, be placed in 25 ℃ of incubators and cultivate, each bacterial strain 3 ware, form, the color of observing bacterium colony.
1.4.2 mycelia and Observations On The Spore Morphology
One sterilizing cover glass was being rigidly connected on the substratum of bacterium with 45° angle oblique cutting, after 25 ℃ of constant temperature culture 3d, cover glass is extracted, microscopic examination the (Xu Fuyuan that takes pictures, Zhang Pei, Zhao Julin etc. utilize bark beetle to release the research [J] that passes muscardine technology prevention pine nematode. forestry scientific research, 2000,13 (specially): forestry scientific research the 22nd volume).
1.4.3 colony growth rate is measured
With the filter paper that the diameter of sterilizing is 6mm, dip in full spore liquid and be placed on culture dish central authorities, be placed in 25 ℃ of incubators and cultivate, with right-angled intersection method, survey colony diameter every day, within the 5th day, play beginning, survey altogether 6 days.Wild type strain, for contrast, repeats 3 times.
1.4.4 mycelia dry weight is measured
The bottled 100mL PD of 250mL taper substratum, draws 0.2mL spore suspension in every bottle of substratum, and constant-temperature table temperature is 25 ℃, and rotating speed is 120r/min.Cultivate after 4d, pour out all bacterium liquid from triangular flask, centrifugal supernatant, is poured on precipitation on filter paper, and 60 ℃ of oven for drying, to constant weight, are weighed.
1.4.5 sporulation quantity is measured
With the punch tool that diameter is 6mm, cut 3 of bacterium colonies and contain in the solution of 0.5 ‰ tweens in 5mL, fully after concussion, with blood counting chamber, count under the microscope, calculate sporulation quantity, establish 3 repetitions.
2 results and analysis
The impact of 2.1 Flight Mutagenesis on colonial morphology and pigment change
On PDA flat board, the cultural characters that different isolated strains show is different, and (Fig. 1, table 1) differs greatly with original strain.Bacterium colony is generally velveteen shape or flocculence, surperficial white or oyster white, and the pale yellow powder of the generation gradually shape spore having, the back side is tawny, some has Vandyke brown ring-type or gully shape lines to exist.
The colony morphology characteristic of table 1 beauveria bassiana space flight mutant
2.2 mycelia and Observations On The Spore Morphology
Beauveria bassiana space flight mutant strain is by microscopic examination; the conidiophore that can see original strain raw on vegetative hyphae; conidiogenous cell clusters on mycelia, conidiophore or the sporangiocyst that expands; doleiform, neck obviously extends into product spore axle, has little tooth prominent on axle; be "the" shape bending; conidiogenous cell and sporangiocyst Chang Zengsheng, on conidiophore or mycelia, conglomerate is to avette quite closely knit spore head, and conidium is spherical or subsphaeroidal.Mutant strain B130 mycelia generally vertically grows to same direction, and mycelia is long and straight, does not produce spore.The mycelia form of B159 is compared with original strain, and barrier film is obvious, and mycelia is slightly crooked between each barrier film.The mycelia of mutant strain B305 and spore shape and original strain no significant difference (Fig. 2).
Through observing in mutant strain, only there are 3 plant mutant strains (B130, B159 and B305) to there is certain product spore ability, the mensuration of further this 3 plant mutant strain being carried out the speed of growth, mycelia dry weight and producing spore amount.
The impact of 2.3 Flight Mutagenesis on the speed of growth
The speed of growth of beauveria bassiana Flight Mutagenesis bacterial strain is compared with original strain, has occurred positive and negative two direction variation, and variation amplitude is large (Fig. 3), illustrates that Flight Mutagenesis is all influential to the trend of the speed of growth of bacterium colony and amplitude.Each colony diameter has growth more than 2mm average every day, belongs to growth fraction type more rapidly.Cultivate after 10d, the colony diameter of bacterial strain B305 is all greater than original strain; Wherein the fastest bacterial strain of the speed of growth is B305, and colony diameter is 4.72cm, and that the speed of growth is the slowest is bacterial strain B159, and colony diameter is 2.67cm.
The table 2 Flight Mutagenesis beauveria bassiana speed of growth
Note: in table, data are mean+SD.In same hurdle, after data, lowercase represents to detect mutual significant difference (P<0.05) through SPSS.
The impact of 2.4 Flight Mutagenesis on mycelia dry weight
Test-results is shown in Fig. 4, and muscardine mutants which had culturing process mycelia dry weight rangeability is little, cultivates after 4d, and the B305 of the bacterial strain that mycelia dry weight is the heaviest is 0.8492g.
The impact of 2.5 Flight Mutagenesis on sporulation quantity
There is the variation (Fig. 5) of different trends, different amplitudes in the bacterial strain after Flight Mutagenesis.That wherein sporulation quantity is maximum is bacterial strain B305, is 2.12 * 10
7cfu/mL; It is less that bacterial strain B159 produces spore, and bacterial strain B130 produces spore hardly.
The Pathogenic Tests of experimental example 2 beauveria bassiana space flight mutant strains to Monochamus alternatus Hope larva
1. experimental technique
In Dalongshan Area forest farm, Anqing, Anhui Province, gather Monochamus alternatus Hope (Monochamus alternatus Hope) larva, take and survive the winter 4 instar larvaes as supplying examination material, indoor feeding 2d, reject band physical abuse and illegal act often individual, select larva healthy, that size is basically identical for biological assay test
Part bacterial strain spore is diluted to 1.0 * 10
7cfu/mL
-1the spore suspension of concentration, compares with sterilized water.Monochamus alternatus Hope larva is flooded to 15s in spore suspension; then being placed in sterile petri dish allows it freely creep; make its body surface without water droplet; then every Monochamus alternatus Hope is placed in the dactylethrae of diameter 12mm * high 75mm; one test tube one cephalont, is equipped with artificial diet in pipe, with the absorbent cotton sealing of soaking sterilized water; be placed in respectively dark culturing under 25 ℃ of envrionment conditionss, 30 larvas of every processing; With equivalent sterilized water, replace spore suspension in contrast simultaneously.By sky observed and recorded roundheaded borer death condition; After death moisturizing of worm is cultivated and is observed mycelial growth and produce spore situation, grows macroscopic mycelia and spore and counts infected (bombys batryticatus), statistics mortality ratio and bombys batryticatus rate on nymph corpse.
2, experimental result
After inoculation, each mutant is different to the virulence of Monochamus alternatus Hope larva, and Flight Mutagenesis is larger on virulence impact, occurs the more of negative sense sudden change.Within the 4th day, larva starts death, and the virulence of mutant B305 increases than original strain, in lethal speed, also faster than original strain, illustrates that mutant B305 has stronger virulence to Monochamus alternatus Hope larva.
The virulence of table 3 beauveria bassiana space flight mutant to Monochamus alternatus Hope four-age larva
The effect experiment of experimental example 3 space flight mutant strain B305 bacterial strain control longicorns
1. test materials and method
From Lin Zhongxuan, getting obvious Monochamus alternatus Hope larva, to invade the pine section in hole some, take back indoorly, are divided into 3 groups, every group of 10-15 section; By the B305 bacterial strain filtering out and beauveria bassiana wild strain (not carrying out Flight Mutagenesis) production spore powder respectively (3.0 * 10
10/ g), adopt respectively and on juggle surface, spread bacterium powder and compare test to two kinds of methods of preventing and treating of injecting bacterium liquid in invading hole.Spore powder consumption is as the criterion on juggle with uniformly dispersing, and the bacterial concentration that injects intrusion hole is 1 * 10
7individual/ml, the consumption of every hole 1ml(B305 bacterial strain and beauveria bassiana wild strain equates).Control group is not done any control and is processed, and control group and test group are separated.After 40 days, break juggle, check one by one.
2. experimental result
Experimental result is in Table 4.
The application comparison of the indoor different strains of table 4 and different methods
From experimental result, no matter be to adopt to spread bacterium powder method or injection bacterium liquid method, space flight mutant strain B305 bacterial strain is all significantly higher than beauveria bassiana wild strain to the lethality rate of longicorn.
Claims (9)
1. a strain beauveria bassiana (Beauveria bassiana (Bals.) Vuill.) Flight Mutagenesis mutant strain B305, is characterized in that, its microbial preservation number is: CGMCC No.8049.
2. the application of Flight Mutagenesis mutant strain B305 claimed in claim 1 in pest control.
3. according to application claimed in claim 2, it is characterized in that: described insect is Monochamus alternatus Hope (Monochamus alternates Hope).
4. a microbial preparation for pest control, is characterized in that, comprising: the spore powder of Flight Mutagenesis mutant strain B305 claimed in claim 1 or fermented liquid and preparations carrier.
5. according to microbial preparation claimed in claim 4, it is characterized in that: described plant insect is Monochamus alternatus Hope (Monochamus alternates Hope).
6. according to microbial preparation claimed in claim 4, it is characterized in that: described preparations carrier is diatomite, kaolin, wood chip, activated carbon, the peat composed of rotten mosses, agricultural crop straw or dry farm manure.
7. according to microbial preparation claimed in claim 4, it is characterized in that: also contain auxiliary material or auxiliary agent.
8. according to microbial preparation claimed in claim 7, it is characterized in that: described auxiliary material is crab shell powder or chitin; Described auxiliary agent is wetting agent, dispersion agent or stablizer.
9. a method of preparing claim 4-8 microbial preparation described in any one, comprises the following steps: the spore powder of Flight Mutagenesis mutant strain B305 or fermented liquid and carrier are mixed, stir, pulverize and sieve, obtain corresponding microbial preparation.
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| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN109988718A (en) * | 2019-05-14 | 2019-07-09 | 西南林业大学 | A kind of Paecilomyces lilacinus category fungal bacterial strain and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105441332A (en) * | 2015-11-19 | 2016-03-30 | 大连理工大学 | Beauveria bassiana separated from tussah and application of beauveria bassiana |
| CN106007824A (en) * | 2016-05-23 | 2016-10-12 | 湖南泰谷生物科技股份有限公司 | Compound bacterial fertilizer as well as preparation method and application thereof |
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| CN107347922A (en) * | 2017-08-18 | 2017-11-17 | 临沂市农业科学院 | A kind of application of Strain of Beauveria bassiana |
| CN109988718A (en) * | 2019-05-14 | 2019-07-09 | 西南林业大学 | A kind of Paecilomyces lilacinus category fungal bacterial strain and its application |
| CN113913307A (en) * | 2021-12-02 | 2022-01-11 | 济南栖圣农林科技有限公司 | Fusarium canopi for preventing and treating monochamus alternatus and application thereof |
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