CN103592300A - Method of measuring respiration intensity of fresh fruits and vegetables by titrimetric analysis - Google Patents
Method of measuring respiration intensity of fresh fruits and vegetables by titrimetric analysis Download PDFInfo
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- CN103592300A CN103592300A CN201310451699.9A CN201310451699A CN103592300A CN 103592300 A CN103592300 A CN 103592300A CN 201310451699 A CN201310451699 A CN 201310451699A CN 103592300 A CN103592300 A CN 103592300A
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- vegetables
- fruits
- standard solution
- exsiccator
- titration
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Abstract
The invention relates to a method of measuring respiration intensity of fresh fruits and vegetables by titrimetric analysis. The method comprises the following steps: (1) placing a partitioning plate with leak holes in a dryer, placing the dryer in a closed box and vacuumizing and filling nitrogen; (2) preparing 20mL of calibrated NaOH standard liquor; (3) accurately weighing 0.500-1.000kg of fruits and vegetables; (4) adding the NaOH standard liquor into the dryer, and then placing the fruits and vegetables in the dryer, wherein the fruits and vegetables are located on the partitioning plate; (5) standing for a period of time and taking out the fruits and vegetables, adding 5mL of saturated barium chloride liquid and two drops of a phenolphthalein indicator into the dryer, and then titrating with 0.1mol.L<-1> oxalic acid standard liquor and marking the volume use level of the oxalic acid standard liquor; and (6) carrying out blank titration. The method provided by the invention is simple, high in measuring accuracy, less in relative standard deviation (RSD), accurate and reliable in test result and practicable, so that the storing effect of the fruits and vegetables is improved.
Description
Technical field
The present invention relates to a kind of assay method, relate in particular to a kind of method that analysis by titration is measured fresh fruit of vegetables respiratory intensity.
Background technology
Fresh fruit and vegetables are after harvesting, and its respiratory intensity becomes a strong and weak important indicator of metabolism.Respiration is contacting various physiology courses directly, indirectly, and therefore also impact is transported and storage effect.Respiration is more vigorous, various processes and variation are faster, life stops also just more early, this has illustrated that fruit and vegetable is in transportation and storage, it is very important effectively controlling respiration, and therefore, respiratory intensity is one of important indicator of research preservation technique, the respiratory intensity of Accurate Determining fruit and vegetable can be weighed respiratory power, for the storage research of fruits and vegetables provides technical effective support.Although there is analysis by titration at present, due in shifting the process of residue alkali lye, residue alkali lye is exposed in air, and in air, contain carbon dioxide, and meeting and residue alkaline reaction, thus the consumption that acid solution titration remains alkali lye affected, cause measuring accuracy rate low, affect measurement result.
Summary of the invention
The present invention has overcome the deficiencies in the prior art, provides a kind of analysis by titration accurately and reliably to measure the method for fresh fruit of vegetables respiratory intensity.
For achieving the above object, the technical solution used in the present invention is: a kind of analysis by titration is measured the method for fresh fruit of vegetables respiratory intensity, comprises the following steps:
(1) in exsiccator, put into the dividing plate with small opening, described exsiccator is placed in a closed box, vacuumize rear inflated with nitrogen;
(2) the NaOH standard solution 20mL that preparation was demarcated;
(3) accurately take the fruits and vegetables of 0.500-1.000kg;
(4) described NaOH standard solution is added in described exsiccator, then described fruits and vegetables are inserted in described exsiccator described fruits and vegetables are positioned on described dividing plate;
(5) after standing certain hour, take out described fruits and vegetables, toward adding 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in described exsiccator, then use 0.1molL
-1the titration of oxalic acid standard solution, write down the volumetric usage of oxalic acid standard solution;
(6) do blank titration.
In a preferred embodiment of the present invention, the method for analysis by titration mensuration fresh fruit of vegetables respiratory intensity is further included in described step (1) and also comprises described exsiccator is cleaned, dried before.
In a preferred embodiment of the present invention, the method that analysis by titration is measured fresh fruit of vegetables respiratory intensity further comprises that, in described step (2), the concentration that NaOH standard solution was demarcated is 0.1994molL
-1.
In a preferred embodiment of the present invention, the method that analysis by titration is measured fresh fruit of vegetables respiratory intensity further comprises in described step (4), by transfer pipet or Syringe injector, described NaOH standard solution is added in described exsiccator.
In a preferred embodiment of the present invention, the method that analysis by titration is measured fresh fruit of vegetables respiratory intensity further comprises in described step (5), takes out described fruits and vegetables after standing 1h.
In a preferred embodiment of the present invention, the method that analysis by titration is measured fresh fruit of vegetables respiratory intensity further comprises that described step (6) specifically comprises the following steps:
(6.1) separately get an exsiccator, described exsiccator is placed in a closed box, vacuumize rear inflated with nitrogen;
(6.2) the NaOH standard solution 20mL that preparation was demarcated;
(6.3) described NaOH standard solution is added in described exsiccator;
(6.4) toward adding 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in described exsiccator, then use 0.1molL
-1the titration of oxalic acid standard solution, write down the volumetric usage of oxalic acid standard solution;
(6.5) repeating said steps (6.1)-(6.4) once, twice or repeatedly, average, draw oxalic acid standard solution blank titration amount.
The invention solves the defect existing in background technology, the inventive method is simple, and the accuracy rate of measuring is high, relative standard deviation RSD is little, test result accurately and reliably, practical, by the respiratory intensity of Accurate Determining fruits and vegetables, can weigh respiratory power, for the storage research of fruits and vegetables provides technical effective support, thereby improve the effect of fruit vegetables storing.
Embodiment
The present invention is further detailed explanation in conjunction with the embodiments now.
A, measuring principle
Quantitatively aqueous slkali absorbs fruits and vegetables and breathes and the CO that discharges in uniform temperature and time internal cause
2, with the remaining alkali of benchmark acidometric titration of preparing, thereby calculate the CO being discharged by breathing
2amount, be the respiratory intensity of fruits and vegetables, unit is mgkg
-1h
-1, its chemical equation is:
2NaOH+CO
2→Na
2CO
3+H
2O；
Na
2CO
3+BaCl
2→BaCO
3+2NaCl；
2NaOH+H
2C
2O
4→Na
2C
2O
4+2H
2O
B, assay method
Analysis by titration is measured a method for fresh fruit of vegetables respiratory intensity, comprises the following steps:
(1) prepare six exsiccators, clean, dry.
(2) in each exsiccator, put into the dividing plate with small opening, six exsiccators are placed in six closed boxs, closed box has transparent visual window, vacuumizes rear inflated with nitrogen, between dividing plate and the inwall of exsiccator, has space, and dividing plate has frame.
(3) prepare six parts of NaOH standard solution 20mL that demarcated.
0.4molL
-1naoH standard training liquid preparation and demarcation.Preparation: NaOH is mixed with to saturated training liquid, and in injected plastic container, airtight to be placed to solution limpid, draws its supernatant solution 10mL in the container of 1000mL, is not diluted to scale with the water containing CO2, shakes up.Demarcate: accurately take primary standard substance Potassium Hydrogen Phthalate 0.4084g, this Potassium Hydrogen Phthalate needs in 110 ℃ of baking ovens, to dry to constant weight in advance, then move in 300mL beaker, add and boil cooled water 100mL and make its dissolving, finally add 2 of 1% phenolphthalein indicator liquid, with the NaOH solution preparing, drop to solution and become redness, be terminal, it is 10.03mL that this test consumes NaOH liquor capacity.1mgmo1
-1kHC8H404 is 0.2042g, and calculating the NaOH solution concentration of demarcating is 0.1994molL
-1.
(4) accurately weigh in the balance and get six parts of fruits and vegetables, the quality of every part of fruits and vegetables is 0.500kg, and fruits and vegetables are preferably fresh cumquat.
(5) six parts of NaOH standard solution are added in six exsiccators by transfer pipet respectively, but being not limited to transfer pipet, can be also Syringe injector, then fresh cumquat is placed on dividing plate through closed box, because dividing plate has frame, fresh cumquat can not fallen in NaOH standard solution.
(6) after standing 1h, take out fresh cumquat, can go out by pliers gripping, adopt transfer pipet toward adding 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in exsiccator, now the solution in exsiccator presents redness, then by 0.1molL
-1oxalic acid standard solution by buret, splash in exsiccator, until red, disappear and finish titration, write down the volumetric usage of oxalic acid standard solution, be respectively 36.73ml, 36.62ml, 36.66ml, 36.61ml, 36.80ml, 36.70ml.
(7) do blank titration.
First, separately get three exsiccators, exsiccator is placed in to an airtight casing, vacuumize rear inflated with nitrogen; Prepare three parts of NaOH standard solution 20mL that demarcated, the same step of set-up procedure (3); Then three parts of NaOH standard solution are added respectively in three exsiccators, by transfer pipet or Syringe injector; Then, toward adding respectively 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in three exsiccators, now the solution in exsiccator presents redness, then by 0.1molL
-1oxalic acid standard solution by buret, splash in exsiccator, until red, disappear and finish titration, write down the volumetric usage of three oxalic acid standard solution, is respectively 38.54ml, 38.55ml, 38.59ml, average, show that oxalic acid standard solution blank titration amount is 38.56ml.
Above temperature environment is 10 ℃, and NaOH standard solution in closed environment, is avoided contacting with air always, guarantees the accurate of titer.
Then with formula calculating below: respiratory intensity (CO
2mgkg
-1h
-1)=[(V1-V2) * C * 44]/(G * t).In formula:
V1 represents the oxalic acid standard solution volume that the blank aqueous slkali of titration is used, and unit is mL;
V2 represents the oxalic acid standard solution volume that titration sample aqueous slkali is used, and unit is mL;
C represents H
2c
2o
4volumetric molar concentration, unit is moLL-1;
44 represent CO
2molal weight, unit is g/mol;
G represents fruits and vegetables sample quality, and unit is kg;
T represents that aqueous slkali absorbs CO in exsiccator
2time, unit is h.
The respiratory intensity CO of sequentially determining
2mgkg
-1h
-1value is respectively 16.1,17.1,16.7,17.2,15.5 and 16.4, and mean value is 16.5CO
2mgkg
-1h
-1, relative standard deviation is 3.9%.Show the result measured with the method accurately and reliably, practical.
Above foundation desirable embodiment of the present invention is enlightenment, and by above-mentioned description, related personnel can, within not departing from the scope of this invention technological thought, carry out various change and modification completely.The technical scope of this invention is not limited to the content on instructions, must determine technical scope according to claim scope.
Claims (6)
1. analysis by titration is measured a method for fresh fruit of vegetables respiratory intensity, it is characterized in that, comprises the following steps:
(1) in exsiccator, put into the dividing plate with small opening, described exsiccator is placed in a closed box, vacuumize rear inflated with nitrogen;
(2) the NaOH standard solution 20mL that preparation was demarcated;
(3) accurately take the fruits and vegetables of 0.500-1.000kg;
(4) described NaOH standard solution is added in described exsiccator, then described fruits and vegetables are inserted in described exsiccator described fruits and vegetables are positioned on described dividing plate;
(5) after standing certain hour, take out described fruits and vegetables, toward adding 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in described exsiccator, then use 0.1molL
-1the titration of oxalic acid standard solution, write down the volumetric usage of oxalic acid standard solution;
(6) do blank titration.
2. analysis by titration according to claim 1 is measured the method for fresh fruit of vegetables respiratory intensity, it is characterized in that, in described step (1), also comprises described exsiccator is cleaned, dried before.
3. analysis by titration according to claim 1 is measured the method for fresh fruit of vegetables respiratory intensity, it is characterized in that, in described step (2), the concentration that NaOH standard solution was demarcated is 0.1994 molL
-1.
4. analysis by titration according to claim 1 is measured the method for fresh fruit of vegetables respiratory intensity, it is characterized in that, in described step (4), by transfer pipet or Syringe injector, described NaOH standard solution is added in described exsiccator.
5. analysis by titration according to claim 1 is measured the method for fresh fruit of vegetables respiratory intensity, it is characterized in that, in described step (5), takes out described fruits and vegetables after standing 1h.
6. analysis by titration according to claim 1 is measured the method for fresh fruit of vegetables respiratory intensity, it is characterized in that, described step (6) specifically comprises the following steps:
(6.1) separately get an exsiccator, described exsiccator is placed in a closed box, vacuumize rear inflated with nitrogen;
(6.2) the NaOH standard solution 20mL that preparation was demarcated;
(6.3) described NaOH standard solution is added in described exsiccator;
(6.4) toward adding 2 of saturated barium chloride solution 5mL and phenolphthalein indicator liquid in described exsiccator, then use 0.1molL
-1the titration of oxalic acid standard solution, write down the volumetric usage of oxalic acid standard solution;
(6.5) repeating said steps (6.1)-(6.4) once, twice or repeatedly, average, draw oxalic acid standard solution blank titration amount.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310451699.9A CN103592300A (en) | 2013-09-27 | 2013-09-27 | Method of measuring respiration intensity of fresh fruits and vegetables by titrimetric analysis |
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|---|---|---|---|
| CN201310451699.9A CN103592300A (en) | 2013-09-27 | 2013-09-27 | Method of measuring respiration intensity of fresh fruits and vegetables by titrimetric analysis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502555A (en) * | 2014-12-27 | 2015-04-08 | 山东商业职业技术学院 | Portable intelligent fruit and vegetable respiration intensity measuring device and method |
| CN105784949A (en) * | 2016-05-03 | 2016-07-20 | 成都亿信标准认证集团有限公司 | Food quality automatic detection device |
| CN112114097A (en) * | 2020-09-22 | 2020-12-22 | 刘骏 | Method for identifying difference of storage tolerance among grape varieties |
| CN112986486A (en) * | 2021-02-26 | 2021-06-18 | 三门峡市质量技术监督检验测试中心 | Method for detecting content of metallic aluminum in aluminum slag deoxidizer and sample preparation method |
-
2013
- 2013-09-27 CN CN201310451699.9A patent/CN103592300A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104502555A (en) * | 2014-12-27 | 2015-04-08 | 山东商业职业技术学院 | Portable intelligent fruit and vegetable respiration intensity measuring device and method |
| CN104502555B (en) * | 2014-12-27 | 2016-04-20 | 山东商业职业技术学院 | A kind of portable intelligent fruits and vegetables respiratory intensity determinator and assay method |
| CN105784949A (en) * | 2016-05-03 | 2016-07-20 | 成都亿信标准认证集团有限公司 | Food quality automatic detection device |
| CN112114097A (en) * | 2020-09-22 | 2020-12-22 | 刘骏 | Method for identifying difference of storage tolerance among grape varieties |
| CN112986486A (en) * | 2021-02-26 | 2021-06-18 | 三门峡市质量技术监督检验测试中心 | Method for detecting content of metallic aluminum in aluminum slag deoxidizer and sample preparation method |
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Application publication date: 20140219 |