CN103592247A - Device and method for detecting pesticide residues in fruits and vegetables - Google Patents

Device and method for detecting pesticide residues in fruits and vegetables Download PDF

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Publication number
CN103592247A
CN103592247A CN201310632266.3A CN201310632266A CN103592247A CN 103592247 A CN103592247 A CN 103592247A CN 201310632266 A CN201310632266 A CN 201310632266A CN 103592247 A CN103592247 A CN 103592247A
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module
reagent
detection
fruits
sample chamber
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CN201310632266.3A
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Chinese (zh)
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郭狄
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ZHONGSHAN DINGSHENG BIOTECHNOLOGY Co Ltd
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ZHONGSHAN DINGSHENG BIOTECHNOLOGY Co Ltd
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Abstract

The invention relates to a device and a method for detecting pesticide residues in fruits and vegetables. The device comprises a detection device upper cover (1) and a detection device main body (2) connected to the detection device upper cover (1). The device is characterized in that the detection device main body (2) comprises a power supply module (3), a reagent storage module (4), a detected sample chamber (5), an input module (6), a detection module (7), a computing module (6), and an output module (9). The device is a portable detection device adopting an enzyme reagent method and can realizes the whole detection on pesticide residues in fruits and vegetables. The device has a high integrated level and realizes convenient and effective detection on pesticide residues in agricultural products such as fruits and vegetables outside of the laboratory.

Description

The checkout equipment of remains of pesticide and detection method in a kind of fruits and vegetables
Technical field
The present invention relates to a kind of checkout equipment, be specifically related to checkout equipment and the detection method of remains of pesticide in a kind of fruits and vegetables.
Technical background
Food security is more and more subject to people's attention in recent years.Along with agrotechnical development, agricultural chemicals is more and more used in the production of fruit and vegetable.Due to also more and more serious to the use of the agricultural chemicals phenomenon of excessive use in crops or pesticide abuse that causes lack of standardization, and the agricultural chemicals that excessive use agricultural chemicals causes is surveyed residue problem and can be affected the healthy of people greatly.At present, in the conventional agricultural chemicals of China, organophosphate and carbamate pesticide has accounted for the more than 60% of the total consumption of agricultural chemicals, organophosphate and carbamate pesticide can suppress the activity of acetylcholinesterase in biosome, make to lose the ability that in catalysis tissue, neurotransmitter acetylcholine is decomposed, thereby acetylcholine is excessively accumulated at neural process synapsis, make a series ofly to take nerve that acetylcholine is conductive medium in the state of being overexcited, cause Nerve conduction disorderly, thereby produce, suppress and exhaustion, cause toxicity symptom.
At present more to the detection method of residues of pesticides, but most of checkout equipments are expensive, although enzyme reagent method cost is lower, but testing process step is more, process is more loaded down with trivial details, be unfavorable for on-the-spot detection rapidly, if enzyme reagent method is realized in same checkout equipment, will greatly improve the efficiency that enzyme reagent method detects.
Summary of the invention
For solving the above-mentioned technical matters existing in prior art, the object of this invention is to provide a kind of method that enzyme reagent method is detected to remains of pesticide and inherit in a portable detection equipment to realize the checkout equipment of remains of pesticide in the fruits and vegetables of fast detecting and the detection method of using this equipment to detect remains of pesticide in fruits and vegetables.
Technical scheme of the present invention is as follows:
The checkout equipment of remains of pesticide in a kind of fruits and vegetables, by pick-up unit upper cover 1, the pick-up unit body 2 being connected with pick-up unit upper cover forms, described detection body 2 comprises power module 3, reagent storage module 4, sample chamber 5 to be checked, load module 6, detection module 7, computing module 8, output module 9 forms, wherein said power module 1 provides electric power for checkout equipment, reagent storage module 4 is for placing detection reagent, reagent in reagent storage module 4 is connected with sample chamber 5 to be checked by pipeline, can reagent be drawn in sample chamber 5 to be checked by pipeline, in sample chamber 5 to be checked, be provided with testing sample box and mixing arrangement, mixing arrangement can mix the liquid in testing sample box, detection module 7 detects for treating the sample of inspection sample chamber 5, load module 6 is for arranging test data and inputting computing module 8, computing module 8 is for receiving the test data of load module 6 inputs, control reagent storage module 4, operation in sample chamber 5 to be checked and detection module 7, calculate the test data that detection module 7 detects, and technology for detection result is input in output module 9.
Preferably, described power module 3 provides electric power by rechargeable battery and/or alternating current module.
Preferably, be provided with 2 ~ 5 reagent storage chambers in described reagent storage module 4, can put into the reagent bottle of standard in reagent storage chamber, the liquid delivery tube in reagent storage module stretches in reagent bottle by the bottle cap of reagent bottle, realizes the extraction to reagent.
Adopt the benefit of standard reagent bottle to be, various detection reagent is put into standard reagent bottle, can change in time and do not affect the carrying out of test when the reagent in fruits and vegetables in the checkout equipment of remains of pesticide is finished.
Preferably, in described reagent storage module, be provided with vector weighing equipment, for measuring the extracted amount that detects reagent.
Preferably, the testing sample box in described sample chamber to be checked 5 is dismountable testing sample box;
Or described mixing arrangement is magnetic stirring apparatus, for the solution of testing sample box is uniformly mixed.
Adopt the benefit of magnetic stirring apparatus to be, equipment connection is simple, does not need to stretch into stirrer in testing sample box, avoids disturbing detecting.
Preferably, described detection module 7 is spectrophotometer.
Preferably, described spectrophotometer is placed along the length direction of pick-up unit body 2, and the laser beam that spectrophotometer penetrates regulates direction to reflex to the direction of sample chamber 5 to be checked by catoptron.
After adding catoptron to regulate the direction of laser beam in spectrophotometer, can make spectrophotometric placement direction more reasonable, thereby the more effective inner space that utilizes the checkout equipment of remains of pesticide in fruits and vegetables, reaches the object that reduces the cumulative volume of the checkout equipment of remains of pesticide in fruits and vegetables.
Preferably, described computing module 8 is microcomputer or single-chip microcomputer, and on preferred described computing module 8, being provided with USB interface can output in other equipment detecting data.
Preferably, described load module 6 is load button, and described output module 9 is LCDs.
Adopt the checkout equipment of remains of pesticide in above-mentioned fruits and vegetables to detect the method for remains of pesticide in fruits and vegetables, comprise the following steps: prepare testing sample and detect reagent, and dependence test information exchange is crossed to load module 6 test parameter is inputted in computing module 8, computing module is controlled the operational throughput of the detection reagent in reagent storage module 4 and is carried kind to input in sample chamber 5 to be checked, after mixing in sample chamber 5, the parameter that computing module 8 is controlled in detection module 7 detects the detection reagent in sample chamber and test result is fed back in computing module 8, then add testing sample to arrive sample chamber to be checked even with detection reagent mix, the parameter that computing module 8 is controlled in detection module 7 detects and test result is fed back in computing module 8 potpourri of the testing sample in sample chamber and detection reagent, computing module 8 is by contrasting the test data of twice, calculate testing result and be shown in output module 9 and complete testing process.
Compared to existing technology, beneficial effect of the present invention is:
1. the present invention is integrated into enzyme reagent method in a portable detection equipment, only need to adopt the checkout equipment of remains of pesticide in these portable fruits and vegetables just can complete whole remains of pesticide testing process.Checkout equipment integrated level of the present invention is high, can make the testing process of remains of pesticide in traditional fruits and vegetables walk out laboratory, realizes the convenient effective detection of pesticide residue on agricultural product.
2. in the present invention, adopt standard reagent bottle, various detection reagent is put into standard reagent bottle, can change in time and do not affect the carrying out of test when the reagent in fruits and vegetables in the checkout equipment of remains of pesticide is finished.
3. the present invention adopts vector weighing equipment to measure the detection reagent extracting, and makes the extraction of various reagent and mixing more accurate.
4. the beam direction that the present invention penetrates spectrophotometer is adjusted, make spectrophotometric placement more reasonable, thereby the more effective inner space that utilizes the checkout equipment of remains of pesticide in fruits and vegetables, reaches the object that reduces the cumulative volume of the checkout equipment of remains of pesticide in fruits and vegetables.
 
Accompanying drawing explanation
Fig. 1 is the structural representation of the checkout equipment of remains of pesticide in fruits and vegetables of the present invention.
Wherein 1 is pick-up unit upper cover; 2 is pick-up unit body; 3 is power module; 4 is reagent storage module; 5 is sample chamber to be checked; 6 is load module; 7 is detection module; 8 is computing module; 9 is output module.
 
Embodiment
Below in conjunction with embodiment, the present invention will be further described:
Embodiment 1
The checkout equipment of remains of pesticide in a kind of fruits and vegetables, by pick-up unit upper cover 1, the pick-up unit body 2 being connected with pick-up unit upper cover forms, described detection body 2 comprises power module 3, reagent storage module 4, sample chamber 5 to be checked, load module 6, detection module 7, computing module 8, output module 9 forms, wherein said power module 1 provides electric power for checkout equipment, reagent storage module 4 is for placing detection reagent, reagent in reagent storage module 4 is connected with sample chamber 5 to be checked by pipeline, can reagent be drawn in sample chamber 5 to be checked by pipeline, in sample chamber 5 to be checked, be provided with testing sample box and mixing arrangement, mixing arrangement can mix the liquid in testing sample box, detection module 7 detects for treating the sample of inspection sample chamber 5, load module 6 is for arranging test data and inputting computing module 8, computing module 8 is for receiving the test data of load module 6 inputs, control reagent storage module 4, operation in sample chamber 5 to be checked and detection module 7, calculate the test data that detection module 7 detects, and technology for detection result is input in output module 9.
Described power module 3 provides electric power by rechargeable battery; In described reagent storage module 4, be provided with 3 reagent storage chambers, can put into the reagent bottle of standard in reagent storage chamber, the liquid delivery tube in reagent storage module stretches in reagent bottle by the bottle cap of reagent bottle, realizes the extraction to reagent.In described reagent storage module, be provided with vector weighing equipment, for measuring the extracted amount that detects reagent.Testing sample box in described sample chamber to be checked 5 is dismountable testing sample box; Described mixing arrangement is magnetic stirring apparatus, for the solution of testing sample box is uniformly mixed.Described detection module 7 is spectrophotometer, and spectrophotometer is placed along the length direction of pick-up unit body 2, and the laser beam that spectrophotometer penetrates regulates direction to reflex to the direction of sample chamber 5 to be checked by catoptron.Described computing module 8 is microcomputer, and on described computing module 8, being provided with USB interface can output in other equipment detecting data; Described load module 6 is load button, and described output module 9 is LCDs.
Adopt the checkout equipment of remains of pesticide in above-mentioned fruits and vegetables to detect the method for remains of pesticide in fruits and vegetables, comprise the following steps: prepare testing sample and detect reagent, and dependence test information exchange is crossed to load module 6 test parameter is inputted in computing module 8, computing module is controlled the operational throughput of the detection reagent in reagent storage module 4 and is carried kind to input in sample chamber 5 to be checked, after mixing in sample chamber 5, the parameter that computing module 8 is controlled in detection module 7 detects the detection reagent in sample chamber and test result is fed back in computing module 8, then add testing sample to arrive sample chamber to be checked even with detection reagent mix, the parameter that computing module 8 is controlled in detection module 7 detects and test result is fed back in computing module 8 potpourri of the testing sample in sample chamber and detection reagent, computing module 8 is by contrasting the test data of 2 times, calculate testing result and be shown in output module 9 and complete testing process.
Embodiment 2
The checkout equipment of remains of pesticide in a kind of fruits and vegetables, by pick-up unit upper cover 1, the pick-up unit body 2 being connected with pick-up unit upper cover forms, described detection body 2 comprises power module 3, reagent storage module 4, sample chamber 5 to be checked, load module 6, detection module 7, computing module 8, output module 9 forms, wherein said power module 1 provides electric power for checkout equipment, reagent storage module 4 is for placing detection reagent, reagent in reagent storage module 4 is connected with sample chamber 5 to be checked by pipeline, can reagent be drawn in sample chamber 5 to be checked by pipeline, in sample chamber 5 to be checked, be provided with testing sample box and mixing arrangement, mixing arrangement can mix the liquid in testing sample box, detection module 7 detects for treating the sample of inspection sample chamber 5, load module 6 is for arranging test data and inputting computing module 8, computing module 8 is for receiving the test data of load module 6 inputs, control reagent storage module 4, operation in sample chamber 5 to be checked and detection module 7, calculate the test data that detection module 7 detects, and technology for detection result is input in output module 9.
Described power module 3 provides electric power by rechargeable battery and alternating current module; In described reagent storage module 4, be provided with 5 reagent storage chambers, can put into the reagent bottle of standard in reagent storage chamber, the liquid delivery tube in reagent storage module stretches in reagent bottle by the bottle cap of reagent bottle, realizes the extraction to reagent.In described reagent storage module, be provided with vector weighing equipment, for measuring the extracted amount that detects reagent.Testing sample box in described sample chamber to be checked 5 is dismountable testing sample box; Described mixing arrangement is magnetic stirring apparatus, for the solution of testing sample box is uniformly mixed.Described detection module 7 is spectrophotometer, and spectrophotometer is placed along the length direction of pick-up unit body 2, and the laser beam that spectrophotometer penetrates regulates direction to reflex to the direction of sample chamber 5 to be checked by catoptron.Described computing module 8 is single-chip microcomputer, and on described computing module 8, being provided with USB interface can output in other equipment detecting data; Described load module 6 is load button, and described output module 9 is LCDs.
Adopt the checkout equipment of remains of pesticide in above-mentioned fruits and vegetables to detect the method for remains of pesticide in fruits and vegetables, comprise the following steps: prepare testing sample and detect reagent, and dependence test information exchange is crossed to load module 6 test parameter is inputted in computing module 8, computing module is controlled the operational throughput of the detection reagent in reagent storage module 4 and is carried kind to input in sample chamber 5 to be checked, after mixing in sample chamber 5, the parameter that computing module 8 is controlled in detection module 7 detects the detection reagent in sample chamber and test result is fed back in computing module 8, then add testing sample to arrive sample chamber to be checked even with detection reagent mix, the parameter that computing module 8 is controlled in detection module 7 detects and test result is fed back in computing module 8 potpourri of the testing sample in sample chamber and detection reagent, computing module 8 is by contrasting the test data of 2 times, calculate testing result and be shown in output module 9 and complete testing process.
Embodiment 3
The checkout equipment of remains of pesticide in a kind of fruits and vegetables, by pick-up unit upper cover 1, the pick-up unit body 2 being connected with pick-up unit upper cover forms, described detection body 2 comprises power module 3, reagent storage module 4, sample chamber 5 to be checked, load module 6, detection module 7, computing module 8, output module 9 forms, wherein said power module 1 provides electric power for checkout equipment, reagent storage module 4 is for placing detection reagent, reagent in reagent storage module 4 is connected with sample chamber 5 to be checked by pipeline, can reagent be drawn in sample chamber 5 to be checked by pipeline, in sample chamber 5 to be checked, be provided with testing sample box and mixing arrangement, mixing arrangement can mix the liquid in testing sample box, detection module 7 detects for treating the sample of inspection sample chamber 5, load module 6 is for arranging test data and inputting computing module 8, computing module 8 is for receiving the test data of load module 6 inputs, control reagent storage module 4, operation in sample chamber 5 to be checked and detection module 7, calculate the test data that detection module 7 detects, and technology for detection result is input in output module 9.
Described power module 3 provides electric power by rechargeable battery and alternating current module; In described reagent storage module 4, be provided with 2 reagent storage chambers, can put into the reagent bottle of standard in reagent storage chamber, the liquid delivery tube in reagent storage module stretches in reagent bottle by the bottle cap of reagent bottle, realizes the extraction to reagent.In described reagent storage module, be provided with vector weighing equipment, for measuring the extracted amount that detects reagent.Testing sample box in described sample chamber to be checked 5 is dismountable testing sample box; Described mixing arrangement is magnetic stirring apparatus, for the solution of testing sample box is uniformly mixed.Described detection module 7 is spectrophotometer, and spectrophotometer is placed along the length direction of pick-up unit body 2, and the laser beam that spectrophotometer penetrates regulates direction to reflex to the direction of sample chamber 5 to be checked by catoptron.Described computing module 8 is microcomputer machine, and described load module 6 is load button, and described output module 9 is LCDs.
Adopt the checkout equipment of remains of pesticide in above-mentioned fruits and vegetables to detect the method for remains of pesticide in fruits and vegetables, comprise the following steps: prepare testing sample and detect reagent, and dependence test information exchange is crossed to load module 6 test parameter is inputted in computing module 8, computing module is controlled the operational throughput of the detection reagent in reagent storage module 4 and is carried kind to input in sample chamber 5 to be checked, after mixing in sample chamber 5, the parameter that computing module 8 is controlled in detection module 7 detects the detection reagent in sample chamber and test result is fed back in computing module 8, then add testing sample to arrive sample chamber to be checked even with detection reagent mix, the parameter that computing module 8 is controlled in detection module 7 detects and test result is fed back in computing module 8 potpourri of the testing sample in sample chamber and detection reagent, computing module 8 is by contrasting the test data of 2 times, calculate testing result and be shown in output module 9 and complete testing process.
The above embodiment is preferred embodiments of the present invention, is not to limit the scope of the present invention, and the equivalence of doing according to structure, feature and principle described in the present patent application the scope of the claims therefore all changes or modifies, and all should comprise in patent claim of the present invention.

Claims (10)

1. the checkout equipment of remains of pesticide in fruits and vegetables, by pick-up unit upper cover (1), the pick-up unit body (2) being connected with pick-up unit upper cover forms, it is characterized in that, described detection body (2) comprises power module (3), reagent storage module (4), sample chamber to be checked (5), load module (6), detection module (7), computing module (8), output module (9) forms, wherein said power module (1) provides electric power for checkout equipment, reagent storage module (4) is for placing detection reagent, reagent in reagent storage module (4) is connected with sample chamber to be checked (5) by pipeline, can reagent be drawn in sample chamber to be checked (5) by pipeline, sample chamber to be checked is provided with testing sample box and mixing arrangement in (5), mixing arrangement can mix the liquid in testing sample box, detection module (7) detects for treating the sample of inspection sample chamber (5), load module (6) is for arranging test data and inputting computing module (8), (8 for receiving the test data of load module (6) input for computing module, control reagent storage module (4), operation in sample chamber to be checked (5) and detection module (7), calculate the test data that detection module (7) detects, and technology for detection result is input in output module (9).
2. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, is characterized in that, described power module (3) provides electric power by rechargeable battery and/or alternating current module.
3. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, it is characterized in that, in described reagent storage module (4), be provided with 2 ~ 5 reagent storage chambers, in reagent storage chamber, can put into the reagent bottle of standard, liquid delivery tube in reagent storage module stretches in reagent bottle by the bottle cap of reagent bottle, realizes the extraction to reagent.
4. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, is characterized in that, in described reagent storage module, is provided with vector weighing equipment, for measuring the extracted amount that detects reagent.
5. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, is characterized in that, the testing sample box in described sample chamber to be checked (5) is dismountable testing sample box;
Or described mixing arrangement is magnetic stirring apparatus, for the solution of testing sample box is uniformly mixed.
6. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, is characterized in that, described detection module (7) is spectrophotometer.
7. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 6, it is characterized in that, described spectrophotometer is placed along the length direction of pick-up unit body (2), and the laser beam that spectrophotometer penetrates regulates direction to reflex to the direction of sample chamber 5 to be checked by catoptron.
8. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, it is characterized in that, described computing module (8) is microcomputer or single-chip microcomputer, and on preferred described computing module (8), being provided with USB interface can output in other equipment detecting data.
9. the checkout equipment of remains of pesticide in a kind of fruits and vegetables according to claim 1, is characterized in that, described load module (6) is load button, and described output module (9) is LCDs.
10. adopt as arbitrary in claim 1 ~ 9 as described in fruits and vegetables in the method for remains of pesticide in the checkout equipment detection fruits and vegetables of remains of pesticide, it is characterized in that, comprise the following steps: prepare testing sample and detect reagent, and dependence test information exchange is crossed to load module (6) test parameter is inputted in computing module (8), computing module is controlled the operational throughput of the detection reagent in reagent storage module (4) and is carried kind to input in sample chamber to be checked (5), in sample chamber (after mixing in 5, the parameter that computing module (8) is controlled in detection module (7) detects the detection reagent in sample chamber and test result is fed back in computing module (8), then add testing sample to arrive sample chamber to be checked even with detection reagent mix, computing module (8) is controlled detection module, and (parameter in 7 detects and test result is fed back in computing module (8) the potpourri of the testing sample in sample chamber and detection reagent, computing module (8) is by contrasting the test data of twice, calculate testing result and be shown in output module (9) and complete testing process.
CN201310632266.3A 2013-11-29 2013-11-29 Device and method for detecting pesticide residues in fruits and vegetables Pending CN103592247A (en)

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CN107271369A (en) * 2017-05-27 2017-10-20 成都明途科技有限公司 Detection means for school's food security
CN109839486A (en) * 2019-02-25 2019-06-04 王芳 A kind of fruits and vegetables pesticide simple detection device and application method
CN111879715A (en) * 2020-08-07 2020-11-03 江西省农业科学院农产品质量安全与标准研究所 Quick detection box for pesticide residue in poultry eggs

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CN111879715A (en) * 2020-08-07 2020-11-03 江西省农业科学院农产品质量安全与标准研究所 Quick detection box for pesticide residue in poultry eggs

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Application publication date: 20140219