CN103589637A - Three-dimensional culture system for cells in vitro - Google Patents

Three-dimensional culture system for cells in vitro Download PDF

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Publication number
CN103589637A
CN103589637A CN201310550341.1A CN201310550341A CN103589637A CN 103589637 A CN103589637 A CN 103589637A CN 201310550341 A CN201310550341 A CN 201310550341A CN 103589637 A CN103589637 A CN 103589637A
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cell
cells
vitro
culture system
matrix
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CN201310550341.1A
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Chinese (zh)
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王海涛
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Priority to CN201310550341.1A priority Critical patent/CN103589637A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention belongs to the field of biotechnology, and in particular discloses a three-dimensional culture system for cells in vitro. The three-dimensional culture system for cells in vitro comprises a plate culture dish and is characterized in that the inner surface of the culture dish is packaged by biological matrix, sustentacular cells are inoculated at the upper layer of the biological matrix, and target cells are inoculated at the upper layer of the sustentacular cells. The three-dimensional culture system for cells in vitro solves the problem of three-dimensional space simulation of cell culture in vitro, the target cells can grow in an environment similar to that in vivo, and the fidelity of morphology and structure is kept. so that the reliability of various tests of cells in vitro is facilitated.

Description

Cells in vitro stereoscopic culture system
(1) technical field
The invention belongs to biological technical field, particularly a kind of cells in vitro stereoscopic culture system.
(2) background technology
Traditional cells in vitro culture technique is that cell is inoculated in the culture vessel of plane, cell is laid in the surface of culture vessel, because cell growing environment in health is three-dimensional environment, there is intercellular interaction, so traditional cells in vitro cultural method has changed growing space and the simultaneous cell-cell interaction of cell.
(3) summary of the invention
The present invention, in order to make up the deficiencies in the prior art, provides a kind of cells in vitro stereoscopic culture system that keeps cell original form, improves test reliability.
The present invention is achieved through the following technical solutions:
A stereoscopic culture system, comprises plane culture dish, it is characterized in that: described culture dish internal surface is coated with bio-matrix, and the inoculation of bio-matrix upper strata has sustenticular cell, and the inoculation of sustenticular cell upper strata has object cell.
The present invention utilizes one-tenth Mierocrystalline cellulose, collagen protein, the extracellular matrix components that in body, Various Tissues exists first culture vessel to be coated with, build three-dimensional arrangement, inoculate thereon again inoblast or other may with the cell type of object cell interaction, utilize the biological activity of inoblast or other emiocytosises to support composition, for the vitro culture of object cell provides three-dimensional arrangement support and bioactive ingredients support, analog cell growing environment in vivo at utmost.
More excellent scheme of the present invention is:
Described bio-matrix is for becoming Mierocrystalline cellulose, collagen protein and extracellular matrix components.
Described sustenticular cell is supported the cell of the inoblast of composition or other and object cell interaction for secretion biological activity.
Described object cell is any cell that will study.
The cell of described other and object cell interaction is immunocyte, vascular endothelial cell, muscle cell or neurocyte.
The invention solves the solid space problem of modelling that cells in vitro is cultivated, study intercellular interaction simultaneously, object cell can be grown in the environment in being similar to its body, the fidelity that has kept its form and structure, function also with in body approaches, thereby is conducive to the reliability of its external various tests.
(4) accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further illustrated.
Fig. 1 is structural representation of the present invention.
In figure, 1 object cell, 2 sustenticular cells, 3 bio-matrixes.
(5) embodiment
Accompanying drawing is a kind of specific embodiment of the present invention.This embodiment comprises plane culture dish, and described culture dish internal surface is coated with bio-matrix 3, and bio-matrix 3 upper strata inoculations have sustenticular cell 2, and sustenticular cell 2 upper strata inoculations have object cell 1; Described bio-matrix 3 is for becoming Mierocrystalline cellulose, collagen protein and extracellular matrix components; Described sustenticular cell 2 is inoblast or other and the interactional cell of object cell 1 of secretion biological activity support composition; Described object cell 1 is any cell that will study; Described other and the interactional cell of object cell 1 are immunocyte, vascular endothelial cell, muscle cell or neurocyte.
Plate is coated with the bio-matrixes such as Mierocrystalline cellulose or collagen protein 3, different according to the kind of object cell 1, the inoblast of inoculation different tissue sources, grow after 1-2 days, until cell grow to 60-90% full after, process cell and make it to stop division, but still survival, inoculate the object cell 1 of wanting vitro culture, analog cell three-dimensional environment and coenocorrelation in vivo, makes it to keep its original characteristic in vivo in vitro.

Claims (5)

1. a cells in vitro stereoscopic culture system, comprise plane culture dish, it is characterized in that: described culture dish internal surface is coated with bio-matrix (3), the inoculation of bio-matrix (3) upper strata has sustenticular cell (2), and the inoculation of sustenticular cell (2) upper strata has object cell (1).
2. cells in vitro stereoscopic culture system according to claim 1, is characterized in that: described bio-matrix (3) is for becoming Mierocrystalline cellulose, collagen protein and extracellular matrix components.
3. cells in vitro stereoscopic culture system according to claim 1, is characterized in that: described sustenticular cell (2) is inoblast or other and the interactional cell of object cell (1) of secretion biological activity support composition.
4. cells in vitro stereoscopic culture system according to claim 1, is characterized in that: described object cell (1) is any cell that will study.
5. cells in vitro stereoscopic culture system according to claim 3, is characterized in that: described other and the interactional cell of object cell (1) are immunocyte, vascular endothelial cell, muscle cell or neurocyte.
CN201310550341.1A 2013-11-08 2013-11-08 Three-dimensional culture system for cells in vitro Pending CN103589637A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310550341.1A CN103589637A (en) 2013-11-08 2013-11-08 Three-dimensional culture system for cells in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310550341.1A CN103589637A (en) 2013-11-08 2013-11-08 Three-dimensional culture system for cells in vitro

Publications (1)

Publication Number Publication Date
CN103589637A true CN103589637A (en) 2014-02-19

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CN (1) CN103589637A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047691A (en) * 2016-05-28 2016-10-26 上海大学 3D cell culture system
CN106244525A (en) * 2016-08-16 2016-12-21 中国农业科学院兰州兽医研究所 A kind of myocardial cell and the Combined culture method of sustentacular cell of testis

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CN101448932A (en) * 2006-04-05 2009-06-03 英国开放大学 Three dimensional cell culture
CN101484574A (en) * 2006-04-28 2009-07-15 雷诺维特有限公司 Substrate for the growth of cultured cells in three dimensions
US20100273667A1 (en) * 2006-02-10 2010-10-28 The Regents Of The University Of Michigan Cell culture well-plates having inverted colloidal crystal scaffolds
WO2011041240A1 (en) * 2009-09-30 2011-04-07 The Brigham And Women's Hospital, Inc. Tissue transplant compositions and methods for use
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CN103255097A (en) * 2012-02-16 2013-08-21 中国科学院大连化学物理研究所 Establishment of humanized three-dimensional intestinal mucosa model and application thereof

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US20100273667A1 (en) * 2006-02-10 2010-10-28 The Regents Of The University Of Michigan Cell culture well-plates having inverted colloidal crystal scaffolds
CN101448932A (en) * 2006-04-05 2009-06-03 英国开放大学 Three dimensional cell culture
CN101484574A (en) * 2006-04-28 2009-07-15 雷诺维特有限公司 Substrate for the growth of cultured cells in three dimensions
WO2011041240A1 (en) * 2009-09-30 2011-04-07 The Brigham And Women's Hospital, Inc. Tissue transplant compositions and methods for use
CN102985556A (en) * 2010-04-20 2013-03-20 国际干细胞公司 Physiological methods for isolation of high purity cell populations
CN102399693A (en) * 2011-10-25 2012-04-04 广东工业大学 Simulation three-dimensional cell cultivation device and cultivation method
CN103255097A (en) * 2012-02-16 2013-08-21 中国科学院大连化学物理研究所 Establishment of humanized three-dimensional intestinal mucosa model and application thereof

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郭勇等: "体外培养所形成的细胞外基质对MC3T3-E1 细胞生长和分化的影响", 《CHINESE JOURNAL OF BIOTECHNOLOGY》, vol. 27, no. 11, 25 November 2011 (2011-11-25) *
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106047691A (en) * 2016-05-28 2016-10-26 上海大学 3D cell culture system
CN106047691B (en) * 2016-05-28 2018-06-01 上海大学 A kind of 3D cell culture systems
CN106244525A (en) * 2016-08-16 2016-12-21 中国农业科学院兰州兽医研究所 A kind of myocardial cell and the Combined culture method of sustentacular cell of testis
CN106244525B (en) * 2016-08-16 2019-06-18 中国农业科学院兰州兽医研究所 A kind of Combined culture method of cardiac muscle cell and sustentacular cell of testis

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Application publication date: 20140219