CN103585235A - Preparation method of Berberis fruit extract - Google Patents
Preparation method of Berberis fruit extract Download PDFInfo
- Publication number
- CN103585235A CN103585235A CN201310525393.3A CN201310525393A CN103585235A CN 103585235 A CN103585235 A CN 103585235A CN 201310525393 A CN201310525393 A CN 201310525393A CN 103585235 A CN103585235 A CN 103585235A
- Authority
- CN
- China
- Prior art keywords
- radix berberidis
- berberidis amurensis
- berry extract
- concentration
- amurensis berry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
Belonging to the medicine field, the invention relates to a preparation method of a Berberis fruit extract. The preparation method includes: taking Berberis fruits as raw materials, conducting extraction with 0-30% ethanol, and carrying out macroporous adsorption resin purification twice to obtain the extract comprising a Berberis fruit extract A and a Berberis fruit extract B. The Berberis fruit extract can be combined with a pharmaceutically acceptable carrier or other suitable excipients so as to be prepared into oral medication dosage forms according to conventional methods.
Description
Technical field
The invention belongs to biomedicine field, relate to a kind of preparation method of Chinese crude drug extract, specifically relate to a kind of preparation method of Radix Berberidis Amurensis berry extract.
Background technology
Radix Berberidis Amurensis is really the dry mature fruit of Berberidaceae plant Berberis nummularia Bge. Berberis nummularia Bge..Record in < < Drug Standard of Ministry of Public Health of the Peoples Republic of China Uigurs medicine fascicle > > in 1998.Its property is flat, have stomach invigorating and in, promoting the production of body fluid to quench thirst, the effect of heat-clearing and toxic substances removing.For dyspepsia, under dysentery is rushed down, thirsty, aphtha, pharyngitis.For China, hide the common drug of the minority areas such as illiteracy.But the basic research of relevant Radix Berberidis Amurensis fruit is still very limited, and the follow-up promotion and application of this medical material are restricted.Modern study shows, Radix Berberidis Amurensis really contains the alkaloids compositions such as berberine, jateorhizine, palmatine, and the anthocyan composition such as pelargonidin, enidin.Other chemical compositions are not quite clear; Pharmacological research has no report.
Domestic patent search result, has no Radix Berberidis Amurensis fruit Patents.
Above-mentioned document and patent etc., there is not yet the relevant report of Radix Berberidis Amurensis berry extract preparation method.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of Radix Berberidis Amurensis berry extract.
The present invention is achieved through the following technical solutions:
The present invention is raw materials used: Radix Berberidis Amurensis is really the dry mature fruit of Berberidaceae plant Berberis nummularia Bge. Berberis nummularia Bge..
The preparation method of a kind of Radix Berberidis Amurensis berry extract of the present invention, is characterized in that preparing according to the following steps:
(1) Radix Berberidis Amurensis fruit, uses concentration 0-30% ethanol as solvent, extracts temperature 50 C-95 ℃, and extraction time is 1-3 time, and each extraction time is 1-4 hour, and each solvent load is 6-15 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.10-1.18, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, pass through nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by polar macroporous resin, use again the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 30%-70%, collect different concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting polar macroporous adsorption resin of concentration 50%-95% again, collect different concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
A preparation method for Radix Berberidis Amurensis berry extract, is characterized in that preparing according to the following steps:
(1) Radix Berberidis Amurensis fruit, as solvent, extracts temperature 70 C with concentration 20% ethanol, and extraction time is 3 times, and each extraction time is 3 hours, and each solvent load is 14 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
A preparation method for Radix Berberidis Amurensis berry extract, is characterized in that preparing according to the following steps:
(1), by Radix Berberidis Amurensis fruit, water is as solvent, heating and refluxing extraction, and extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
The preparation method of a kind of Radix Berberidis Amurensis berry extract of the present invention, is characterized in that: described Radix Berberidis Amurensis berry extract mainly contains: berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.
The preparation method of a kind of Radix Berberidis Amurensis berry extract of the present invention, is characterized in that: the nonpolar macroporous adsorption resin adopting is D101 macroporous adsorbent resin, AB-8 macroporous adsorbent resin; The polar macroporous adsorption resin adopting is LSA-40 macroporous resin, HPD600 macroporous resin.
Radix Berberidis Amurensis berry extract of the present invention, by the various adjuvants that add pharmaceutics to allow, makes the peroral dosage forms such as tablet on pharmaceutics, granule, capsule.
The specific embodiment
Below by specific experiment example and embodiment, the preparation method of a kind of Radix Berberidis Amurensis berry extract of the present invention is described further, but is not limited to the present invention.
Below by specific embodiment, be described in further detail.
Embodiment 1: the preparation of Radix Berberidis Amurensis berry extract and monomer component research
(1) Radix Berberidis Amurensis fruit 20kg, water is as solvent, heating and refluxing extraction, extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
Radix Berberidis Amurensis berry extract carries out silica gel column chromatography, Preparative TLC chromatograph and Sephadex LH mono-2O column chromatography, obtains respectively sloughing off berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.The chemical constitution of each compound is all through wave spectrum means confirmations such as mass spectrum and nuclear magnetic resonance, NMR above, and purity detects and is all greater than 98% through high performance liquid chromatography.
Embodiment 2: the preparation of Radix Berberidis Amurensis berry extract and monomer component research
(1) Radix Berberidis Amurensis fruit 15kg, as solvent, extracts temperature 70 C with concentration 20% ethanol, and extraction time is 3 times, and each extraction time is 3 hours, and each solvent load is 14 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
Radix Berberidis Amurensis berry extract carries out silica gel column chromatography, Preparative TLC chromatograph and Sephadex LH mono-2O column chromatography, obtains respectively sloughing off berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.The chemical constitution of each compound is all through wave spectrum means confirmations such as mass spectrum and nuclear magnetic resonance, NMR above, and purity detects and is all greater than 98% through high performance liquid chromatography.
Embodiment 3: the preparation of Radix Berberidis Amurensis berry extract and monomer component research
(1) Radix Berberidis Amurensis fruit 30kg, as solvent, extracts temperature 50 C with concentration 30% ethanol, and extraction time is 1 time, and each extraction time is 4 hours, and each solvent load is 15 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.18, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by AB-8 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of HPD600, use again the alcoholic solution eluting AB-8 nonpolar macroporous adsorption resin of concentration 70%, collect 70% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting HPD600 polar macroporous adsorption resin of concentration 95% again, collect 95% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
Radix Berberidis Amurensis berry extract carries out silica gel column chromatography, Preparative TLC chromatograph and Sephadex LH mono-2O column chromatography, obtains respectively sloughing off berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.The chemical constitution of each compound is all through wave spectrum means confirmations such as mass spectrum and nuclear magnetic resonance, NMR above, and purity detects and is all greater than 98% through high performance liquid chromatography.
Embodiment 4: the preparation of Radix Berberidis Amurensis berry extract and monomer component research
(1) Radix Berberidis Amurensis fruit 15kg, as solvent, extracts 95 ℃ of temperature with concentration 5% ethanol, and extraction time is 3 times, and each extraction time is 1 hour, and each solvent load is 6 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.10, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 30%, collect 30% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 50% again, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
Radix Berberidis Amurensis berry extract carries out silica gel column chromatography, Preparative TLC chromatograph and Sephadex LH mono-2O column chromatography, obtains respectively sloughing off berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.The chemical constitution of each compound is all through wave spectrum means confirmations such as mass spectrum and nuclear magnetic resonance, NMR above, and purity detects and is all greater than 98% through high performance liquid chromatography.
Embodiment 5: the preparation of Radix Berberidis Amurensis berry extract tablet
Get embodiment 1 Radix Berberidis Amurensis berry extract 235g, add starch 45g, mix, granulate, dry, sieve, add microcrystalline Cellulose 35g, magnesium stearate 3.5g, mixes, and is pressed into 1000, obtains Radix Berberidis Amurensis berry extract tablet.
Embodiment 6: the preparation of Radix Berberidis Amurensis berry extract granule
Get embodiment 2 Radix Berberidis Amurensis berry extract 265g, add dextrin 50g, sucrose 50g, mixes, and granulates, and dry, granulate, obtains Radix Berberidis Amurensis berry extract granule.
Embodiment 7: the preparation of Radix Berberidis Amurensis berry extract capsule
Get embodiment 3 Radix Berberidis Amurensis berry extract 225g, add starch 65g, mix, granulate, dry, granulate,, obtains Radix Berberidis Amurensis berry extract capsule by encapsulated 1000.
Claims (6)
1. a preparation method for Radix Berberidis Amurensis berry extract, is characterized in that preparing according to the following steps:
(1) Radix Berberidis Amurensis fruit, uses concentration 0-30% ethanol as solvent, extracts temperature 50 C-95 ℃, and extraction time is 1-3 time, and each extraction time is 1-4 hour, and each solvent load is 6-15 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.10-1.18, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, pass through nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by polar macroporous resin, use again the alcoholic solution eluting nonpolar macroporous adsorption resin of concentration 30%-70%, collect different concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting polar macroporous adsorption resin of concentration 50%-95% again, collect different concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
2. the preparation method of a kind of Radix Berberidis Amurensis berry extract according to claim 1, is characterized in that preparing according to the following steps:
(1) Radix Berberidis Amurensis fruit, as solvent, extracts temperature 70 C with concentration 20% ethanol, and extraction time is 3 times, and each extraction time is 3 hours, and each solvent load is 14 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
3. the preparation method of a kind of Radix Berberidis Amurensis berry extract according to claim 1, is characterized in that preparing according to the following steps:
(1) Radix Berberidis Amurensis fruit, water is as solvent, heating and refluxing extraction, extraction time is 2 times, and each extraction time is 2 hours, and each solvent load is 12 times of Radix Berberidis Amurensis fruit weight; Filter, merge extractive liquid,, reclaims ethanol, is concentrated into relative density d=1.12, filters, and obtains medicinal liquid A;
(2) medicinal liquid A step (1) being obtained, by D101 nonpolar macroporous adsorption resin, first wash with water, water elution liquid is directly by the polar macroporous resin of LSA-40, use again the alcoholic solution eluting D101 nonpolar macroporous adsorption resin of concentration 50%, collect 50% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract A; Use the alcoholic solution eluting LSA-40 polar macroporous adsorption resin of concentration 80% again, collect 80% concentration ethanol eluent, concentrate drying, obtains Radix Berberidis Amurensis berry extract B;
(3) above-mentioned Radix Berberidis Amurensis berry extract A and Radix Berberidis Amurensis berry extract B are mixed, obtain Radix Berberidis Amurensis berry extract of the present invention.
4. according to the preparation method of claim 1, claim 2, a kind of Radix Berberidis Amurensis berry extract claimed in claim 3, it is characterized in that: described Radix Berberidis Amurensis berry extract mainly contains: berberine, jateorhizine, palmatine, magnoflorine, pelargonidin, enidin.
5. the preparation method of a kind of Radix Berberidis Amurensis berry extract according to claim 1, is characterized in that: the nonpolar macroporous adsorption resin adopting is D101 macroporous adsorbent resin, AB-8 macroporous adsorbent resin; The polar macroporous adsorption resin adopting is LSA-40 macroporous resin, HPD600 macroporous resin.
6. according to claim 1, claim 2, Radix Berberidis Amurensis berry extract claimed in claim 3, by the various adjuvants that add pharmaceutics to allow, make the peroral dosage forms such as tablet on pharmaceutics, granule, capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525393.3A CN103585235B (en) | 2013-10-31 | 2013-10-31 | A kind of preparation method of barberry berry extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525393.3A CN103585235B (en) | 2013-10-31 | 2013-10-31 | A kind of preparation method of barberry berry extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103585235A true CN103585235A (en) | 2014-02-19 |
| CN103585235B CN103585235B (en) | 2016-05-04 |
Family
ID=50075689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310525393.3A Expired - Fee Related CN103585235B (en) | 2013-10-31 | 2013-10-31 | A kind of preparation method of barberry berry extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103585235B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814497A (en) * | 2014-12-01 | 2015-08-05 | 拜城县尼格尔实业发展有限公司 | Berberis nummularia beverage production method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106617042A (en) * | 2016-09-13 | 2017-05-10 | 防城港市天红农业科技有限责任公司 | Preparation method of syzygium samarangense extract |
-
2013
- 2013-10-31 CN CN201310525393.3A patent/CN103585235B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 库尔班江等: "高效液相色谱法测定红果小檗中小檗碱的含量", 《新疆师范大学学报(自然科学版)》, no. 02, 30 June 2008 (2008-06-30) * |
| 林叶新: "《川黄连中小檗碱的提取及纯化工艺的研究》", 《中成药》, vol. 35, no. 6, 30 June 2013 (2013-06-30), pages 1204 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814497A (en) * | 2014-12-01 | 2015-08-05 | 拜城县尼格尔实业发展有限公司 | Berberis nummularia beverage production method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103585235B (en) | 2016-05-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102451235B (en) | Preparation method of olive leaf extract | |
| CN102976909B (en) | Method for extracting and purifying 6-gingerol from ginger | |
| CN101260131A (en) | Method for extracting iridoid active site and monomer from eucommia bark | |
| CN105055527A (en) | Preparation method of olive leaf extract | |
| CN103585311B (en) | A kind of preparation method of Spiraea alpina extract | |
| CN102924416B (en) | Method for separating and purifying monomeric compounds from ash bark | |
| CN104173438A (en) | Preparation method of general flavone of purple perilla | |
| CN106242953A (en) | A kind of method preparing resveratrol from phoenix seed of Flos Moutan shell | |
| CN109295121A (en) | A kind of method that enzymatic isolation method prepares icariin | |
| WO2017067366A1 (en) | Method for extracting and refining alkaloid from ipecac | |
| CN103588831A (en) | Preparation method of glycyrrhizic acid | |
| CN103585235A (en) | Preparation method of Berberis fruit extract | |
| CN104262231B (en) | From white thorn seed, extract the method that separates L-Trp | |
| CN105434539A (en) | Composition of lotus flavones | |
| CN1962650A (en) | Process for preparing high-purity dehydrated andrographolide | |
| CN111001189A (en) | Method for capturing and separating effective components in liquorice by using mixed-mode agarose gel medium | |
| CN102180934A (en) | Preparation method of tubeimoside I | |
| CN102250183B (en) | Method for preparing high-purity ginsenoside Re by using ginseng flower buds as raw materials | |
| CN101618052A (en) | Process for extracting total flavonoids from hippophae leaves | |
| CN105061212B (en) | A kind of preparation method of neochlorogenic acid | |
| CN113440547A (en) | Method for separating and purifying Japanese thistle herb total glycosides by adopting macroporous resin series dynamic axial compression column | |
| CN102399252A (en) | Preparation method for cowherb seed flavonoid glycoside monomer | |
| CN103585187B (en) | A kind of preparation method of wall tapestry extract | |
| CN110437152A (en) | The extraction separation method of magnoflorine in a kind of slenderstalk dicranostigma herb | |
| CN103585210B (en) | The preparation method of Dorema ammoniacum resin total sesquiterpene coumarin extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| CB03 | Change of inventor or designer information |
Inventor after: Sun Chunhui Inventor after: Guo Yunxia Inventor after: Sun Xiuyong Inventor before: Kong Qianqian |
|
| COR | Change of bibliographic data | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160405 Address after: Licang District of Shandong city of Qingdao province Yongping Road 266000 No. 29 Applicant after: No.3 People Hospital, Qingdao City Address before: 250101 No. 51 Industrial South Road, Ji'nan hi tech Zone, Shandong, Ji'nan Applicant before: Jinan Xingyi Medical Technology Co.,Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160504 Termination date: 20181031 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |